MD01G1118300.v1.1

Belongs to the chalcone isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004474
Physical Location & Seq
Reverse (-)
23211441 .. 23212288
848 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1118300.v1.1.491

Sequence Viewer

Length: 468 bp
ATGGCTCCAACGCCATCGCTCGCCGGACTCCAGGTCGAGACGACTGCGTTTCCACCGTCCGCCAAACCTCCGGGCTCCTCTAACACTCTGTTCCTCGGCGGCGCAGGGGTGAGGGGGCTGGAGATTCAGGGGAACTTCGTGAAGTTCACGGCGATCGGAGTGTACTTGGAGGAAAACGCCGTGCCTCTGCTCGCCGTTAAGTGGAAGGGTAAGACGGCCGAGGAGTTGACGGAGTCCGTTGAGTTCTTCAGGGACATCGTTACAGGTAACACGGATTTCTTCGCTGTTTTTAACGACTGGTTGAATTTTGTTACATCATTCATGCCCTGCATCTTCGGGCTGAGCAGTTTTAGAATGATCGGTAGTCATGCCAAACCTAGGCAATCCTATAGCCCGCCCTTCCTCAGTTGCCGATATAGATCTGTACGTTCAACAAATGTCGTCCGTTTGAGAAATTCATTCAAGTGA

Protein Analysis

156

Amino Acids

17.08

Weight (kDa)

9.51

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chalcone PF02431 12 - 93 6.5e-36 Chalcone-flavanone isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 60, 99, 395
AcoI YGGCCR 1 cut(s) 216
AcsI RAATTY 2 cut(s) 304, 454
AcuI CTGAAG 1 cut(s) 232
AfaI GTAC 2 cut(s) 164, 426
AfiI CCNNNNNNNGG 2 cut(s) 201, 378
AgsI TTSAA 3 cut(s) 304, 432, 463
AhdI GACNNNNNGTC 1 cut(s) 32
AjnI CCWGG 1 cut(s) 30
Alw26I GTCTC 1 cut(s) 32
AoxI GGCC 1 cut(s) 216
ApoI RAATTY 2 cut(s) 304, 454
AspA2I CCTAGG 1 cut(s) 377
AspLEI GCGC 1 cut(s) 104
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 1 cut(s) 121
AvrII CCTAGG 1 cut(s) 377
BanII GRGCYC 1 cut(s) 77
BccI CCATC 1 cut(s) 22
BceAI ACGGC 4 cut(s) 164, 165, 179, 231
BcgI CGANNNNNNTGC 2 cut(s) 26, 60
BciT130I CCWGG 1 cut(s) 32
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 1 cut(s) 32
BfaI CTAG 1 cut(s) 378
BfmI CTRYAG 1 cut(s) 388
BglII AGATCT 1 cut(s) 419
BisI GCNGC 1 cut(s) 100
BlnI CCTAGG 1 cut(s) 377
BlpI GCTNAGC 1 cut(s) 341
BlsI GCNGC 1 cut(s) 101
Bme1390I CCNGG 2 cut(s) 32, 72
BmeRI GACNNNNNGTC 1 cut(s) 32
BmiI GGNNCC 2 cut(s) 6, 76
BmrFI CCNGG 2 cut(s) 32, 72
BmsI GCATC 1 cut(s) 339
BpmI CTGGAG 2 cut(s) 14, 140
Bpu1102I GCTNAGC 1 cut(s) 341
BpuMI CCSGG 1 cut(s) 72
BsaBI GATNNNNATC 1 cut(s) 418
BsaJI CCNNGG 3 cut(s) 94, 219, 377
Bsc4I CCNNNNNNNGG 2 cut(s) 201, 378
Bse1I ACTGG 1 cut(s) 302
Bse8I GATNNNNATC 1 cut(s) 418
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 3 cut(s) 94, 219, 377
BseJI GATNNNNATC 1 cut(s) 418
BseLI CCNNNNNNNGG 2 cut(s) 201, 378
BseMII CTCAG 2 cut(s) 332, 418
BseNI ACTGG 1 cut(s) 302
BseRI GAGGAG 2 cut(s) 67, 236
BseX3I CGGCCG 1 cut(s) 216
Bsh1285I CGRYCG 2 cut(s) 156, 219
BshFI GGCC 1 cut(s) 218
BsiEI CGRYCG 2 cut(s) 156, 219
BsiSI CCGG 2 cut(s) 24, 71
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 2 cut(s) 201, 378
BsmAI GTCTC 1 cut(s) 32
BsmBI CGTCTC 1 cut(s) 32
BsmFI GGGAC 1 cut(s) 266
BsnI GGCC 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 77
Bsp143I GATC 3 cut(s) 153, 357, 419
Bsp1720I GCTNAGC 1 cut(s) 341
BspACI CCGC 3 cut(s) 60, 99, 395
BspANI GGCC 1 cut(s) 218
BspCNI CTCAG 2 cut(s) 333, 417
BspLI GGNNCC 2 cut(s) 6, 76
BsrI ACTGG 1 cut(s) 302
BssECI CCNNGG 3 cut(s) 94, 219, 377
BssMI GATC 3 cut(s) 153, 357, 419
BssT1I CCWWGG 1 cut(s) 377
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 1 cut(s) 57
BstC8I GCNNGC 3 cut(s) 21, 192, 395
BstDEI CTNAG 2 cut(s) 341, 404
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 3 cut(s) 156, 360, 422
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 3 cut(s) 153, 357, 419
BstMCI CGRYCG 2 cut(s) 156, 219
BstNI CCWGG 1 cut(s) 32
BstSCI CCNGG 2 cut(s) 30, 70
BstSFI CTRYAG 1 cut(s) 388
BstX2I RGATCY 1 cut(s) 419
BstYI RGATCY 1 cut(s) 419
BstZI CGGCCG 1 cut(s) 216
BsuRI GGCC 1 cut(s) 218
Cac8I GCNNGC 3 cut(s) 21, 192, 395
CfoI GCGC 1 cut(s) 104
Csp6I GTAC 2 cut(s) 163, 425
CviAII CATG 2 cut(s) 322, 368
CviJI RGCY 6 cut(s) 5, 75, 118, 218, 340, 393
CviKI_1 RGCY 6 cut(s) 5, 75, 118, 218, 340, 393
CviQI GTAC 2 cut(s) 163, 425
DdeI CTNAG 2 cut(s) 341, 404
DpnI GATC 3 cut(s) 155, 359, 421
DpnII GATC 3 cut(s) 153, 357, 419
DriI GACNNNNNGTC 1 cut(s) 32
EaeI YGGCCR 1 cut(s) 216
EagI CGGCCG 1 cut(s) 216
Eam1105I GACNNNNNGTC 1 cut(s) 32
EciI GGCGGA 1 cut(s) 49
EclXI CGGCCG 1 cut(s) 216
Eco130I CCWWGG 1 cut(s) 377
Eco24I GRGCYC 1 cut(s) 77
Eco52I CGGCCG 1 cut(s) 216
Eco57I CTGAAG 1 cut(s) 232
EcoRII CCWGG 1 cut(s) 30
EcoT14I CCWWGG 1 cut(s) 377
EcoT38I GRGCYC 1 cut(s) 77
ErhI CCWWGG 1 cut(s) 377
Esp3I CGTCTC 1 cut(s) 32
FaeI CATG 2 cut(s) 325, 371
FaiI YATR 4 cut(s) 323, 369, 390, 417
FaqI GGGAC 1 cut(s) 266
FatI CATG 2 cut(s) 321, 367
FauI CCCGC 1 cut(s) 402
Fnu4HI GCNGC 1 cut(s) 100
FriOI GRGCYC 1 cut(s) 77
Fsp4HI GCNGC 1 cut(s) 100
FspBI CTAG 1 cut(s) 378
GlaI GCGC 1 cut(s) 103
GluI GCNGC 1 cut(s) 100
GsuI CTGGAG 2 cut(s) 14, 140
HaeIII GGCC 1 cut(s) 218
HapII CCGG 2 cut(s) 24, 71
HhaI GCGC 1 cut(s) 104
Hin1II CATG 2 cut(s) 325, 371
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HincII GTYRAC 1 cut(s) 228
HindII GTYRAC 1 cut(s) 228
HinfI GANTC 3 cut(s) 27, 124, 233
HpaII CCGG 2 cut(s) 24, 71
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 3 cut(s) 147, 163, 228
Hpy188I TCNGA 1 cut(s) 158
Hpy188III TCNNGA 2 cut(s) 37, 139
Hpy8I GTNNAC 3 cut(s) 147, 163, 228
HpyAV CCTTC 2 cut(s) 199, 409
HpyCH4III ACNGT 1 cut(s) 57
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 1 cut(s) 330
HpyF3I CTNAG 2 cut(s) 341, 404
HpySE526I ACGT 1 cut(s) 427
Hsp92II CATG 2 cut(s) 325, 371
HspAI GCGC 1 cut(s) 102
Kzo9I GATC 3 cut(s) 153, 357, 419
LmnI GCTCC 2 cut(s) 10, 80
LweI GCATC 1 cut(s) 339
MaeI CTAG 1 cut(s) 378
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 3 cut(s) 259, 266, 310
MalI GATC 3 cut(s) 155, 359, 421
MboI GATC 3 cut(s) 153, 357, 419
MboII GAAGA 3 cut(s) 238, 271, 325
MflI RGATCY 1 cut(s) 419
MhlI GDGCHC 1 cut(s) 77
MluCI AATT 2 cut(s) 304, 454
MlyI GAGTC 2 cut(s) 21, 242
MmeI TCCRAC 1 cut(s) 32
MnlI CCTC 8 cut(s) 78, 88, 104, 105, 163, 195, 214, 413
MseI TTAA 2 cut(s) 198, 291
MslI CAYNNNNRTG 1 cut(s) 463
MspI CCGG 2 cut(s) 24, 71
MspR9I CCNGG 2 cut(s) 32, 72
MvaI CCWGG 1 cut(s) 32
NciI CCSGG 1 cut(s) 72
NdeII GATC 3 cut(s) 153, 357, 419
NlaIII CATG 2 cut(s) 325, 371
NlaIV GGNNCC 2 cut(s) 6, 76
NmeAIII GCCGAG 2 cut(s) 75, 244
PfeI GAWTC 1 cut(s) 124
PflFI GACNNNGTC 1 cut(s) 232
PkrI GCNGC 1 cut(s) 101
Ple19I CGATCG 1 cut(s) 156
PleI GAGTC 2 cut(s) 21, 241
PpsI GAGTC 2 cut(s) 21, 241
Psp6I CCWGG 1 cut(s) 30
PspGI CCWGG 1 cut(s) 30
PspN4I GGNNCC 2 cut(s) 6, 76
PsuI RGATCY 1 cut(s) 419
PsyI GACNNNGTC 1 cut(s) 232
PvuI CGATCG 1 cut(s) 156
RsaI GTAC 2 cut(s) 164, 426
RsaNI GTAC 2 cut(s) 163, 425
RseI CAYNNNNRTG 1 cut(s) 463
SaqAI TTAA 2 cut(s) 198, 291
SatI GCNGC 1 cut(s) 100
Sau3AI GATC 3 cut(s) 153, 357, 419
SchI GAGTC 2 cut(s) 21, 242
ScrFI CCNGG 2 cut(s) 32, 72
SduI GDGCHC 1 cut(s) 77
SetI ASST 5 cut(s) 36, 70, 268, 379, 430
SfaNI GCATC 1 cut(s) 339
SfcI CTRYAG 1 cut(s) 388
SmiMI CAYNNNNRTG 1 cut(s) 463
Sse9I AATT 2 cut(s) 304, 454
SsiI CCGC 3 cut(s) 60, 99, 395
SspMI CTAG 1 cut(s) 378
StyD4I CCNGG 2 cut(s) 30, 70
StyI CCWWGG 1 cut(s) 377
TaaI ACNGT 1 cut(s) 57
TaiI ACGT 1 cut(s) 430
TaqI TCGA 1 cut(s) 36
TasI AATT 2 cut(s) 304, 454
TatI WGTACW 1 cut(s) 162
TauI GCSGC 1 cut(s) 102
TfiI GAWTC 1 cut(s) 124
Tru1I TTAA 2 cut(s) 198, 291
Tru9I TTAA 2 cut(s) 198, 291
TspDTI ATGAA 2 cut(s) 310, 447
TspGWI ACGGA 4 cut(s) 226, 245, 287, 434
Tth111I GACNNNGTC 1 cut(s) 232
XapI RAATTY 2 cut(s) 304, 454
XmaJI CCTAGG 1 cut(s) 377
XspI CTAG 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.