RchiOBHm_Chr7g0221421

Belongs to the chalcone isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004474
Physical Location & Seq
Forward (+)
42475521 .. 42476473
953 bp
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UTR
Exon/CDS
Intron
PRQ19813

Sequence Viewer

Length: 321 bp
ATGGCTCAATCAGTTACCGGAATTCAGGTCGAAGAGACCACGTTTCCTCCCGCAGTCAAGCCTCCGGGCTCCGCCAACACTCTGTTCCTCGGCGGTGCAGGGGCGAGGGGGCTGGAGATACAGGGGAATTTCGTCAGGTTCACGGCGATTGGAGTTTACTTGGAGGATAAGGCCGGGCCGGCGCTTGCCGTTAAGTGGAGTGGAAGGGTAAGACGGCCGAGGAGTCCGTTAGAGAAATTCACACAGGTGACGATGATACTACCATTGACAGGCCAGCAATACTCGGAGAAGGTTTCAGAGAATTGTGTTGCCATTTGGTAA

Protein Analysis

106

Amino Acids

11.44

Weight (kDa)

9.36

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chalcone PF02431 10 - 106 1.6e-35 Chalcone-flavanone isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 51, 72, 93
AcoI YGGCCR 1 cut(s) 215
AcsI RAATTY 3 cut(s) 21, 127, 236
AfiI CCNNNNNNNGG 2 cut(s) 195, 269
AjuI GAANNNNNNNTTGG 2 cut(s) 68, 100
AleI CACNNNNGTG 1 cut(s) 245
Alw26I GTCTC 1 cut(s) 29
AoxI GGCC 4 cut(s) 171, 176, 215, 271
ApoI RAATTY 3 cut(s) 21, 127, 236
AspLEI GCGC 1 cut(s) 184
AspS9I GGNCC 1 cut(s) 176
AsuC2I CCSGG 2 cut(s) 66, 175
AsuHPI GGTGA 1 cut(s) 259
BanII GRGCYC 1 cut(s) 71
BceAI ACGGC 3 cut(s) 159, 173, 230
BcnI CCSGG 2 cut(s) 66, 175
BcoDI GTCTC 1 cut(s) 29
BfoI RGCGCY 1 cut(s) 185
BglI GCCNNNNNGGC 1 cut(s) 179
Bme1390I CCNGG 2 cut(s) 66, 175
BmgT120I GGNCC 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 70
BmrFI CCNGG 2 cut(s) 66, 175
BpmI CTGGAG 1 cut(s) 134
BpuMI CCSGG 2 cut(s) 66, 175
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 88, 218
BsaWI WCCGGW 1 cut(s) 17
BsaXI ACNNNNNCTCC 2 cut(s) 31, 61
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 269
Bse118I RCCGGY 1 cut(s) 178
BseDI CCNNGG 2 cut(s) 88, 218
BseLI CCNNNNNNNGG 2 cut(s) 195, 269
BseRI GAGGAG 1 cut(s) 235
BseX3I CGGCCG 1 cut(s) 215
BsgI GTGCAG 1 cut(s) 117
Bsh1285I CGRYCG 1 cut(s) 218
BshFI GGCC 4 cut(s) 173, 178, 217, 273
BsiEI CGRYCG 1 cut(s) 218
BsiSI CCGG 4 cut(s) 18, 65, 174, 179
BslI CCNNNNNNNGG 2 cut(s) 195, 269
BsmAI GTCTC 1 cut(s) 29
BsnI GGCC 4 cut(s) 173, 178, 217, 273
Bso31I GGTCTC 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 71
BspACI CCGC 3 cut(s) 51, 72, 93
BspANI GGCC 4 cut(s) 173, 178, 217, 273
BspLI GGNNCC 1 cut(s) 70
BspTNI GGTCTC 1 cut(s) 29
BsrFI RCCGGY 1 cut(s) 178
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 2 cut(s) 88, 218
Bst6I CTCTTC 1 cut(s) 27
BstC8I GCNNGC 3 cut(s) 180, 186, 275
BstH2I RGCGCY 1 cut(s) 185
BstHHI GCGC 1 cut(s) 184
BstMAI GTCTC 1 cut(s) 29
BstMCI CGRYCG 1 cut(s) 218
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstSCI CCNGG 2 cut(s) 64, 173
BstZI CGGCCG 1 cut(s) 215
BsuRI GGCC 4 cut(s) 173, 178, 217, 273
Cac8I GCNNGC 3 cut(s) 180, 186, 275
CfoI GCGC 1 cut(s) 184
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 176
CviJI RGCY 8 cut(s) 5, 61, 69, 112, 173, 178, 217, 273
CviKI_1 RGCY 8 cut(s) 5, 61, 69, 112, 173, 178, 217, 273
EaeI YGGCCR 1 cut(s) 215
EagI CGGCCG 1 cut(s) 215
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
EciI GGCGGA 1 cut(s) 61
EclXI CGGCCG 1 cut(s) 215
Eco24I GRGCYC 1 cut(s) 71
Eco31I GGTCTC 1 cut(s) 29
Eco52I CGGCCG 1 cut(s) 215
EcoRI GAATTC 1 cut(s) 21
EcoT38I GRGCYC 1 cut(s) 71
FauI CCCGC 1 cut(s) 58
FriOI GRGCYC 1 cut(s) 71
GlaI GCGC 1 cut(s) 183
GsuI CTGGAG 1 cut(s) 134
HaeII RGCGCY 1 cut(s) 185
HaeIII GGCC 4 cut(s) 173, 178, 217, 273
HapII CCGG 4 cut(s) 18, 65, 174, 179
HhaI GCGC 1 cut(s) 184
Hin6I GCGC 1 cut(s) 182
HinP1I GCGC 1 cut(s) 182
HinfI GANTC 1 cut(s) 223
HpaII CCGG 4 cut(s) 18, 65, 174, 179
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 2 cut(s) 141, 157
Hpy188I TCNGA 2 cut(s) 286, 298
Hpy8I GTNNAC 2 cut(s) 141, 157
HpyAV CCTTC 2 cut(s) 198, 283
HpyCH4IV ACGT 1 cut(s) 41
HpyCH4V TGCA 1 cut(s) 98
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpySE526I ACGT 1 cut(s) 41
HspAI GCGC 1 cut(s) 182
KroI GCCGGC 1 cut(s) 178
KroNI GCCGGC 1 cut(s) 180
LmnI GCTCC 1 cut(s) 74
MaeII ACGT 1 cut(s) 41
MaeIII GTNAC 2 cut(s) 13, 247
MboII GAAGA 1 cut(s) 44
MhlI GDGCHC 1 cut(s) 71
MluCI AATT 4 cut(s) 21, 127, 236, 301
MlyI GAGTC 1 cut(s) 232
MnlI CCTC 6 cut(s) 57, 72, 98, 99, 157, 213
MroNI GCCGGC 1 cut(s) 178
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 1 cut(s) 245
MspI CCGG 4 cut(s) 18, 65, 174, 179
MspR9I CCNGG 2 cut(s) 66, 175
MwoI GCNNNNNNNGC 1 cut(s) 179
NaeI GCCGGC 1 cut(s) 180
NciI CCSGG 2 cut(s) 66, 175
NgoMIV GCCGGC 1 cut(s) 178
NlaIV GGNNCC 1 cut(s) 70
NmeAIII GCCGAG 2 cut(s) 69, 243
NmuCI GTSAC 1 cut(s) 247
OliI CACNNNNGTG 1 cut(s) 245
PdiI GCCGGC 1 cut(s) 180
PleI GAGTC 1 cut(s) 231
PpsI GAGTC 1 cut(s) 231
PspN4I GGNNCC 1 cut(s) 70
PspPI GGNCC 1 cut(s) 176
RseI CAYNNNNRTG 1 cut(s) 245
SaqAI TTAA 1 cut(s) 192
Sau96I GGNCC 1 cut(s) 176
SchI GAGTC 1 cut(s) 232
ScrFI CCNGG 2 cut(s) 66, 175
SduI GDGCHC 1 cut(s) 71
SetI ASST 5 cut(s) 30, 44, 140, 249, 294
SmiMI CAYNNNNRTG 1 cut(s) 245
Sse9I AATT 4 cut(s) 21, 127, 236, 301
SsiI CCGC 3 cut(s) 51, 72, 93
StyD4I CCNGG 2 cut(s) 64, 173
TaiI ACGT 1 cut(s) 44
TaqI TCGA 1 cut(s) 30
TasI AATT 4 cut(s) 21, 127, 236, 301
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TseFI GTSAC 1 cut(s) 247
Tsp45I GTSAC 1 cut(s) 247
TspGWI ACGGA 1 cut(s) 216
XapI RAATTY 3 cut(s) 21, 127, 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.