Rroxscaffold_4G00291420

Belongs to the chalcone isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004474
Physical Location & Seq
Forward (+)
11870366 .. 11872065
1700 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00291420.1

Sequence Viewer

Length: 714 bp
ATGGCTCAATCAGTTACCGGAATTCAGGTCGAAGCGACCACGTTTCCTCCCGCCGTCAAGCCTCCGGGATCCGCCAACACTCTGTTCCTCGCCGGCGCAGGGGCGAGGGGGCTGGAGATACAGGGGAATTTCGTCAAGTTTACGGCGATTGGAGTCTACTTGGAGGATAAGGCCGTGCCGGCGCTCGCCGTTAAGTGGAAGGGTAAGACGGCCGAGGAGTTGACGGAGTCCGTTGAGTTCTTCAGGGAGATCGTTACAGGTCCATTTGAGAAATTCACACAGGTGACGATGATACTACCATTGACTGGCCAGCAATACTCGGAGAAGGTTTCAGAGAATTGTGTTGCCATTTGGAAAAAATTTGGAATTTACACTGATGCAGAAGCCAAAGCTATTGAAAAGTTTACGGAGGTCTTCAAAGATCAGACCTTCCCACCTGGTGCTTCTATTCTCTTCACACAATCACCAAATGGATCATTGACGATTGGCTTCTCCAAAGATGGTTCCATACCAGCAGTTGGAAATGCGGTGATTGAAAACAAGCTACTTTCAGAGTCAGTTCTCGAGTCAATCATTGGAAAGCAAGGTGTTTCTCCTGAAGCAAGGAAATGTGTGGCTACAAGGCTATCAGAATTGTTGAAAGAGAGTGATGATTGCGTGGCCGGAAATGGGAAAGTGGAAGAGTACACAAACGAAGCAGAAGTCAAGGCATGA

Protein Analysis

237

Amino Acids

25.42

Weight (kDa)

4.99

Isoelectric Point (pI)

32.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chalcone PF02431 10 - 212 2.1e-105 Chalcone-flavanone isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 305, 518
AccI GTMKAC 1 cut(s) 156
AciI CCGC 3 cut(s) 51, 72, 527
AclWI GGATC 3 cut(s) 63, 76, 481
AcoI YGGCCR 3 cut(s) 210, 307, 660
AcsI RAATTY 5 cut(s) 21, 127, 272, 359, 366
AcuI CTGAAG 2 cut(s) 226, 618
AdeI CACNNNGTG 1 cut(s) 440
AfaI GTAC 1 cut(s) 686
AfiI CCNNNNNNNGG 5 cut(s) 99, 195, 305, 518, 669
AgsI TTSAA 4 cut(s) 398, 418, 536, 640
AjnI CCWGG 1 cut(s) 436
AjuI GAANNNNNNNTTGG 2 cut(s) 68, 100
AleI CACNNNNGTG 1 cut(s) 281
AluBI AGCT 2 cut(s) 392, 544
AluI AGCT 2 cut(s) 392, 544
AlwI GGATC 3 cut(s) 63, 76, 481
Ama87I CYCGRG 1 cut(s) 563
AoxI GGCC 4 cut(s) 171, 210, 307, 660
ApoI RAATTY 5 cut(s) 21, 127, 272, 359, 366
ArsI GACNNNNNNTTYG 1 cut(s) 687
AspLEI GCGC 2 cut(s) 98, 184
AspS9I GGNCC 1 cut(s) 260
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 3 cut(s) 295, 456, 541
AvaI CYCGRG 1 cut(s) 563
AvaII GGWCC 1 cut(s) 260
BalI TGGCCA 1 cut(s) 309
BamHI GGATCC 1 cut(s) 68
BbsI GAAGAC 1 cut(s) 406
BccI CCATC 1 cut(s) 494
BceAI ACGGC 5 cut(s) 38, 158, 159, 173, 225
BciT130I CCWGG 1 cut(s) 438
BcnI CCSGG 1 cut(s) 66
BfoI RGCGCY 1 cut(s) 185
BglI GCCNNNNNGGC 1 cut(s) 179
Bme1390I CCNGG 2 cut(s) 66, 438
Bme18I GGWCC 1 cut(s) 260
BmeT110I CYCGRG 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 260
BmiI GGNNCC 2 cut(s) 70, 505
BmrFI CCNGG 2 cut(s) 66, 438
BmsI GCATC 1 cut(s) 367
BpiI GAAGAC 1 cut(s) 406
BpmI CTGGAG 1 cut(s) 134
BpuMI CCSGG 1 cut(s) 66
BsaJI CCNNGG 1 cut(s) 213
BsaWI WCCGGW 1 cut(s) 17
BsaXI ACNNNNNCTCC 2 cut(s) 31, 61
Bsc4I CCNNNNNNNGG 5 cut(s) 99, 195, 305, 518, 669
Bse118I RCCGGY 2 cut(s) 92, 178
Bse1I ACTGG 1 cut(s) 310
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 1 cut(s) 213
BseLI CCNNNNNNNGG 5 cut(s) 99, 195, 305, 518, 669
BseNI ACTGG 1 cut(s) 310
BseRI GAGGAG 1 cut(s) 230
BseX3I CGGCCG 1 cut(s) 210
Bsh1285I CGRYCG 1 cut(s) 213
BshFI GGCC 4 cut(s) 173, 212, 309, 662
BsiEI CGRYCG 1 cut(s) 213
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 5 cut(s) 18, 65, 93, 179, 663
BslI CCNNNNNNNGG 5 cut(s) 99, 195, 305, 518, 669
BsnI GGCC 4 cut(s) 173, 212, 309, 662
BsoBI CYCGRG 1 cut(s) 563
Bsp143I GATC 4 cut(s) 68, 249, 421, 473
BspACI CCGC 3 cut(s) 51, 72, 527
BspANI GGCC 4 cut(s) 173, 212, 309, 662
BspLI GGNNCC 2 cut(s) 70, 505
BspPI GGATC 3 cut(s) 63, 76, 481
BsrFI RCCGGY 2 cut(s) 92, 178
BsrI ACTGG 1 cut(s) 310
BssAI RCCGGY 2 cut(s) 92, 178
BssECI CCNNGG 1 cut(s) 213
BssMI GATC 4 cut(s) 68, 249, 421, 473
Bst2UI CCWGG 1 cut(s) 438
Bst6I CTCTTC 2 cut(s) 458, 675
BstC8I GCNNGC 4 cut(s) 94, 180, 186, 311
BstH2I RGCGCY 1 cut(s) 185
BstHHI GCGC 2 cut(s) 98, 184
BstKTI GATC 4 cut(s) 71, 252, 424, 476
BstMBI GATC 4 cut(s) 68, 249, 421, 473
BstMCI CGRYCG 1 cut(s) 213
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 1 cut(s) 438
BstSCI CCNGG 2 cut(s) 64, 436
BstV2I GAAGAC 1 cut(s) 406
BstX2I RGATCY 1 cut(s) 68
BstYI RGATCY 1 cut(s) 68
BstZI CGGCCG 1 cut(s) 210
BsuRI GGCC 4 cut(s) 173, 212, 309, 662
BtsIMutI CAGTG 1 cut(s) 372
Cac8I GCNNGC 4 cut(s) 94, 180, 186, 311
CfoI GCGC 2 cut(s) 98, 184
Cfr10I RCCGGY 2 cut(s) 92, 178
Cfr13I GGNCC 1 cut(s) 260
CsiI ACCWGGT 1 cut(s) 436
Csp6I GTAC 1 cut(s) 685
CviAII CATG 1 cut(s) 711
CviQI GTAC 1 cut(s) 685
DpnI GATC 4 cut(s) 70, 251, 423, 475
DpnII GATC 4 cut(s) 68, 249, 421, 473
DraIII CACNNNGTG 1 cut(s) 440
EaeI YGGCCR 3 cut(s) 210, 307, 660
EagI CGGCCG 1 cut(s) 210
Eam1104I CTCTTC 2 cut(s) 458, 675
EarI CTCTTC 2 cut(s) 458, 675
EciI GGCGGA 1 cut(s) 61
EclXI CGGCCG 1 cut(s) 210
Eco47I GGWCC 1 cut(s) 260
Eco52I CGGCCG 1 cut(s) 210
Eco57I CTGAAG 2 cut(s) 226, 618
Eco88I CYCGRG 1 cut(s) 563
EcoRI GAATTC 1 cut(s) 21
EcoRII CCWGG 1 cut(s) 436
FaeI CATG 1 cut(s) 714
FaiI YATR 2 cut(s) 509, 712
FatI CATG 1 cut(s) 710
FauI CCCGC 1 cut(s) 58
FblI GTMKAC 1 cut(s) 156
GlaI GCGC 2 cut(s) 97, 183
GsuI CTGGAG 1 cut(s) 134
HaeII RGCGCY 1 cut(s) 185
HaeIII GGCC 4 cut(s) 173, 212, 309, 662
HapII CCGG 5 cut(s) 18, 65, 93, 179, 663
HhaI GCGC 2 cut(s) 98, 184
Hin1II CATG 1 cut(s) 714
Hin6I GCGC 2 cut(s) 96, 182
HinP1I GCGC 2 cut(s) 96, 182
HincII GTYRAC 1 cut(s) 222
HindII GTYRAC 1 cut(s) 222
HinfI GANTC 4 cut(s) 153, 227, 554, 566
HpaII CCGG 5 cut(s) 18, 65, 93, 179, 663
HphI GGTGA 3 cut(s) 295, 456, 541
Hpy166II GTNNAC 5 cut(s) 141, 157, 222, 405, 687
Hpy188I TCNGA 5 cut(s) 322, 334, 426, 553, 631
Hpy188III TCNNGA 2 cut(s) 563, 596
Hpy8I GTNNAC 5 cut(s) 141, 157, 222, 405, 687
HpyAV CCTTC 3 cut(s) 193, 319, 439
HpyCH4IV ACGT 1 cut(s) 41
HpyCH4V TGCA 1 cut(s) 380
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpySE526I ACGT 1 cut(s) 41
Hsp92II CATG 1 cut(s) 714
HspAI GCGC 2 cut(s) 96, 182
KroI GCCGGC 2 cut(s) 92, 178
KroNI GCCGGC 2 cut(s) 94, 180
Kzo9I GATC 4 cut(s) 68, 249, 421, 473
LweI GCATC 1 cut(s) 367
MabI ACCWGGT 1 cut(s) 436
MaeII ACGT 1 cut(s) 41
MaeIII GTNAC 3 cut(s) 13, 253, 283
MalI GATC 4 cut(s) 70, 251, 423, 475
MboI GATC 4 cut(s) 68, 249, 421, 473
MboII GAAGA 4 cut(s) 232, 406, 445, 692
MflI RGATCY 1 cut(s) 68
MlsI TGGCCA 1 cut(s) 309
MluCI AATT 7 cut(s) 21, 127, 272, 337, 359, 366, 632
MluNI TGGCCA 1 cut(s) 309
MlyI GAGTC 4 cut(s) 162, 236, 563, 575
MmeI TCCRAC 1 cut(s) 499
MnlI CCTC 7 cut(s) 57, 72, 98, 99, 157, 208, 403
Mox20I TGGCCA 1 cut(s) 309
MreI CGCCGGCG 1 cut(s) 92
MroNI GCCGGC 2 cut(s) 92, 178
MscI TGGCCA 1 cut(s) 309
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 1 cut(s) 281
Msp20I TGGCCA 1 cut(s) 309
MspI CCGG 5 cut(s) 18, 65, 93, 179, 663
MspR9I CCNGG 2 cut(s) 66, 438
MvaI CCWGG 1 cut(s) 438
MwoI GCNNNNNNNGC 1 cut(s) 179
NaeI GCCGGC 2 cut(s) 94, 180
NciI CCSGG 1 cut(s) 66
NdeII GATC 4 cut(s) 68, 249, 421, 473
NgoMIV GCCGGC 2 cut(s) 92, 178
NlaIII CATG 1 cut(s) 714
NlaIV GGNNCC 2 cut(s) 70, 505
NmeAIII GCCGAG 1 cut(s) 238
NmuCI GTSAC 1 cut(s) 283
OliI CACNNNNGTG 1 cut(s) 281
PaeR7I CTCGAG 1 cut(s) 563
PdiI GCCGGC 2 cut(s) 94, 180
PflFI GACNNNGTC 1 cut(s) 226
PflMI CCANNNNNTGG 2 cut(s) 305, 518
PfoI TCCNGGA 1 cut(s) 64
PleI GAGTC 4 cut(s) 161, 235, 562, 574
PpsI GAGTC 4 cut(s) 161, 235, 562, 574
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 2 cut(s) 70, 505
PspPI GGNCC 1 cut(s) 260
PsuI RGATCY 1 cut(s) 68
PsyI GACNNNGTC 1 cut(s) 226
RsaI GTAC 1 cut(s) 686
RsaNI GTAC 1 cut(s) 685
RseI CAYNNNNRTG 1 cut(s) 281
SaqAI TTAA 1 cut(s) 192
Sau3AI GATC 4 cut(s) 68, 249, 421, 473
Sau96I GGNCC 1 cut(s) 260
SchI GAGTC 4 cut(s) 162, 236, 563, 575
ScrFI CCNGG 2 cut(s) 66, 438
SexAI ACCWGGT 1 cut(s) 436
SfaNI GCATC 1 cut(s) 367
Sfr274I CTCGAG 1 cut(s) 563
SgrAI CRCCGGYG 1 cut(s) 92
SinI GGWCC 1 cut(s) 260
SlaI CTCGAG 1 cut(s) 563
SmiMI CAYNNNNRTG 1 cut(s) 281
SmlI CTYRAG 1 cut(s) 563
SmoI CTYRAG 1 cut(s) 563
Sse9I AATT 7 cut(s) 21, 127, 272, 337, 359, 366, 632
SsiI CCGC 3 cut(s) 51, 72, 527
StyD4I CCNGG 2 cut(s) 64, 436
TaiI ACGT 1 cut(s) 44
TaqI TCGA 2 cut(s) 30, 564
TasI AATT 7 cut(s) 21, 127, 272, 337, 359, 366, 632
TatI WGTACW 1 cut(s) 684
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 1 cut(s) 379
TseFI GTSAC 1 cut(s) 283
Tsp45I GTSAC 1 cut(s) 283
TspGWI ACGGA 3 cut(s) 220, 239, 422
TspRI CASTG 1 cut(s) 379
Tth111I GACNNNGTC 1 cut(s) 226
Van91I CCANNNNNTGG 2 cut(s) 305, 518
VpaK11BI GGWCC 1 cut(s) 260
XapI RAATTY 5 cut(s) 21, 127, 272, 359, 366
XhoI CTCGAG 1 cut(s) 563
XmiI GTMKAC 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.