Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001811

Physical Location & Seq

Genomic Coordinates Forward (+)

23587720 .. 23592951

Gene Structure 5232 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1122800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3060 bp

ATGTCGTGGTTTAGAGCTGGGTCTGGTGTGGCAAAGCTCGCCATTAGGAGAAGTTTATCGAATAGCGGGTCCTATGCGGCTAGGAGGCGGGTTCTTCCGTCCCAAAGCCGAGACTTTCACACCACAATACCTAAATCAAAGGCTGCCCCGGTTCCACGCCCTGTACCCCTCTCTAGGCTAACTGATAGTTTCTTAGATGGAACCAGCAGTGTTTATTTGGAGGGGCTTCAGAGGGCTTGGGAAGCCGACCCCAATAGCGTTGATGAGTCGTGGGACAATTTCTTTAGGAATTTTGTGGGTCAGGCCTCTACCTCGCCCGGAATTTCGGGGCAGACCATTCAAGAGAGTATGCGTCTGTTGTTGCTTGTGAGGGCATACCAGGTCAATGGTCACATGAAAGCTAAATTGGACCCTTTGGGTTTGGAAGAGAGAGAAATCCCAGATGATCTTGACCCTGCTCTATATGGGTTCACTGAGGCTGATCTCGATAGAGAGTTCTTTTTGGGGGTCTGGAGGATGGCTGGGTTTTTGTCTGAGAACCGTCCTGTGCAGACCCTTAGGTCCATATTGACCCGGCTTGAGCAGGCTTACTGTGGGACCATTGGGTATGAGTATATGCACATTGCAGATCGTGAGAGATGTAACTGGTTGAGAAACAAGATTGAGACTCCTATACCAATGCAATATAATAGGCAGCGCCGTGAGGTTATTCTTGATAGGCTTATTTGGAGTACACAGTTTGAGAACTTCTTAGCCACTAAGTGGACAGCAGCAAAGAGGTTTGGGCTTGAAGGGGGAGAAACTCTTATTCCTGGCATGAAGGAGATGTTTGATAGGTCTGCTGATCTCGGGGTTGAGAGCATAGTTATTGGAATGTCTCACAGAGGCAGACTGAATGTATTGGGTAATGTGGTTCGGAAACCTTTGCGTCACATATTTAGTGAATTCAGTGGTGGAACAAAGCCCGTGGATGAAGATGGGCTCTACACAGGGACTGGTGATGTCAAGTATCACCTGGGAACCTCTTATGATCGACCCACTAGAGGTGGGAAGAGAATCCATTTATCTTTGGTTGCAAATCCAAGTCACTTGGAAGCTGTGGACCCCGTTGTTGTTGGAAAAACTAGAGCAAAACAATATTATTCAAATGATGCGGACAGGACCAAGAATATGGGTGTCTTGATTCATGGAGATGGTAGCTTTGCTGGACAAGGTGTGGTCTACGAGACTCTTCATCTAAGTGCTCTTCCGAACTATACCACTGGTGGGACTATACACATTGTGGTGAACAACCAAGTTGCGTTCACAACTGATCCAATGTCCGGAAGATCGTCACAGTATTGTACTGATGTTGCCAAAGCATTGAATGCCCCTATTTTTCATGTAAATGCTGATGACGTGGAGGCAGTTGTTCATGTATGTGAGCTTGCAGCTGAATGGCGCCAGACCTTCCATTCTGATGTTGTGGTTGATTTAGTGTGCTATCGTCGATTTGGGCATAATGAGATTGATGAGCCCTCTTTCACGCAGCCCAAAATGTACAAGGTTATTCGGAATCATCCATCAGCTCTAACAATCTACCAGAACAAACTTCTAGAGTCTGGACAAGTAGCCAAAGAAGACATTGAAAGGATACAAAGCAAGGTTAATGAAATTCTCAATGAAGAATTCTTGGCTAGCAAGGATTACACTCTTCAAAGAAGGGACTGGCTTTCATCCCATTGGTCTGGATTTAAGTCACCTGAACAGATTTCTCGTATCCGGAATACTGGGGTAAAACCAGAGATTTTGAAGAGTGTTGGCAAAGCAGTTACAGCCCTTCCAGAAACTTTAAAGCCTCACAGGGCAGTAAAGAAGGTGTATGAACAACGTGCACAAATGATTGCAACTGGTGAAGGAATTGATTGGGCAGTTGCGGAAGCACTTGCCTTTGCTACACTACTAGTAGAAGGTAACCATGTTCGACTGAGTGGTCAGGATGTTGAAAGAGGTACATTTAGTCATCGGCATTCTGTTATTCACGACCAGGAAACTGGGGAAAAGTATTGCCCTCTTGACCATATTACGGCTGGCCAAGATGAGGAAATGTTTACAGTTAGCAACAGCTCACTCTCGGAGTTTGGAGTTCTTGGATTTGAATTGGGTTACTCGATGGAGAGTCCAAATGCATTAGTGATCTGGGAAGCTCAATTTGGTGATTTTGCAAACGGAGCTCAAGTTATCTTTGATCAGTTTTTGAGTAGTGGGGAGTCCAAGTGGCTTCGTCAAACTGGGCTTGTTGTGCTGCTTCCTCATGGCTACGATGGCCAGGGCCCTGAACATTCTAGTGCCCGACTGGAGCGATTTCTTCAGATGAGTGATGACCATCCTTTTGTCATACCTGAGATGGATCCCACACTTCGAAAGCAAATTCAGGAATGCAATTGGCAGGTTGTGAACGTGACAACTCCTGCCAACTACTTCCATGTTCTGCGTCGTCAGTTACACAGGGAATTCCGCAAGCCTCTTATTGTGATGGCTCCTAAAAACCTGTTACGGCACAAGGATTGCAAATCAAATCTATCTGAGTTTGATGATGTGCAAGGTCACCCAGGTTTTGACAAGCAGGGGACCAGATTTAAGCGCCTCATCAAGGACCAGAATGGCCACTCTGATCTGGAGGAGGGTATTAGACGGCTGGTCCTGTGCTCCGGCAAGGTTTATTATGAGCTTGATGAAGAGCGAAGAAAGGTTGAAGCAAAGGATGTTGCAATTTGTAGAGTGGAACAGCTTTGCCCTTTCCCGTATGACCTCATCCAGAGAGAGCTGAAGCGATATCCAAATGCTGAGATAGTTTGGGTCCAGGAAGAGCCGATGAACATGGGCGCCTACAGCTATATTGCACCCCGCCTTAGCACTGCCATGAAGTCCCTGGGTAGAGGAACTTTCGACGACATAAAATATATTGGCCGTGCTCCATCAGCTGCCACGGCCACTGGTTTCTATACAGTTCATTTGAAGGAACAAAGTGAGATTGTACACAAAGCGGTACAGAAGGAGCCAATTGAGTTTCCCAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004591 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005947 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0008150 GO:0008152 GO:0009060 GO:0009353 GO:0009987 GO:0015980 GO:0016491 GO:0016624 GO:0016903 GO:0016999 GO:0017144 GO:0019752 GO:0030062 GO:0031974 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045239 GO:0045240 GO:0045252 GO:0045333 GO:0055114 GO:0070013 GO:0071704 GO:0072350 GO:0098798 GO:1902494 GO:1990204 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00020 ko00310 ko00380 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map00380 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

2-oxogl_dehyd_N E1_dh OxoGdeHyase_C Transket_pyr

Protein Analysis

1020

Amino Acids

115.19

Weight (kDa)

6.58

Isoelectric Point (pI)

43.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-oxogl_dehyd_N	PF16078	62 - 99	3.7e-14	2-oxoglutarate dehydrogenase N-terminus
E1_dh	PF00676	243 - 564	8.7e-58	Dehydrogenase E1 component
Transket_pyr	PF02779	633 - 846	8e-49	Transketolase, pyrimidine binding domain
OxoGdeHyase_C	PF16870	869 - 1009	2.2e-49	2-oxoglutarate dehydrogenase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001811)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55410 AT3G55410 AT5G65750
fragaria_vesca	FvH4_7g21770 FvH4_7g21770 FvH4_7g21770 FvH4_7g21770 FvH4_7g21770 FvH4_7g21770 FvH4_7g21851
malus_domestica	MD01G1122800.v1.1 MD02G1281300.v1.1 MD02G1281500.v1.1 MD07G1192300.v1.1
prunus_persica	Prupe.2G230300_v2.0.a1 Prupe.2G230400_v2.0.a1 Prupe.2G230400_v2.0.a1 Prupe.2G230400_v2.0.a1
pyrus_communis	pycom01g14980 pycom02g24050 pycom07g18180
rosa_chinensis	RchiOBHm_Chr1g0365921 RchiOBHm_Chr1g0366031
rosa_laevigata	RLG00000027377 RLG00000027378
rosa_multiflora	Rmu_co8265029.1_g000001 Rmu_sc0004288.1_g000005 Rmu_sc0006406.1_g000008
rosa_roxburghii	Rroxscaffold_4G00290620 Rroxscaffold_4G00290640
rosa_rugosa	Rorug01G0325800 Rorug01G0325900 Rorug01G0326000 Rorug01G0326100 Rorug01G0326200 Rorug01G0326300 Rorug01G0326400
rosa_samantha	Rh1AG334500 Rh1AG334600 Rh1BG295400 Rh1BG295600 Rh1CG312100 Rh1CG312200 Rh1DG327700 Rh1DG328000
rosa_wichuraiana	Rw1G029690 Rw1G029720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	559, 1971
Acc36I	ACCTGC	1 cut(s)	2419
AccB1I	GGYRCC	2 cut(s)	1440, 2864
AccB7I	CCANNNNNTGG	1 cut(s)	762
AccI	GTMKAC	1 cut(s)	1221
AccIII	TCCGGA	2 cut(s)	1322, 1761
AciI	CCGC	8 cut(s)	66, 77, 88, 1154, 1916, 2497, 2887, 3026
AclWI	GGATC	3 cut(s)	1307, 2384, 2397
AcoI	YGGCCR	5 cut(s)	2071, 2305, 2644, 2947, 2970
AcsI	RAATTY	7 cut(s)	289, 321, 944, 1653, 1667, 2409, 2492
AcuI	CTGAAG	3 cut(s)	212, 2333, 2828
AcyI	GRCGYC	2 cut(s)	1441, 2865
AdeI	CACNNNGTG	2 cut(s)	762, 1282
AfaI	GTAC	7 cut(s)	165, 733, 1345, 1541, 1993, 3018, 3030
AfiI	CCNNNNNNNGG	8 cut(s)	174, 762, 1043, 1266, 1322, 2065, 2080, 2632
AhdI	GACNNNNNGTC	1 cut(s)	2678
AhlI	ACTAGT	1 cut(s)	1942
AjiI	CACGTC	1 cut(s)	1399
AjnI	CCWGG	8 cut(s)	378, 811, 1014, 2025, 2307, 2590, 2841, 2910
AjuI	GAANNNNNNNTTGG	4 cut(s)	389, 421, 2175, 2207
Alw21I	GWGCWC	5 cut(s)	1246, 1876, 2215, 2690, 2956
Alw26I	GTCTC	4 cut(s)	105, 659, 882, 1220
Alw44I	GTGCAC	1 cut(s)	1872
AlwI	GGATC	3 cut(s)	1307, 2384, 2397
AlwNI	CAGNNNCTG	1 cut(s)	995
Ama87I	CYCGRG	1 cut(s)	848
Aor13HI	TCCGGA	2 cut(s)	1322, 1761
AoxI	GGCC	7 cut(s)	303, 2071, 2305, 2311, 2644, 2947, 2970
ApaI	GGGCCC	1 cut(s)	2315
ApaLI	GTGCAC	1 cut(s)	1872
ApeKI	GCWGC	7 cut(s)	143, 694, 770, 1430, 1528, 2284, 2963
ApoI	RAATTY	7 cut(s)	289, 321, 944, 1653, 1667, 2409, 2492
ArsI	GACNNNNNNTTYG	4 cut(s)	53, 85, 2354, 2386
AspLEI	GCGC	4 cut(s)	699, 1443, 2625, 2867
AsuC2I	CCSGG	3 cut(s)	149, 318, 574
AsuHPI	GGTGA	7 cut(s)	1004, 1010, 1297, 1731, 1904, 2207, 2579
AsuII	TTCGAA	1 cut(s)	2401
AsuNHI	GCTAGC	1 cut(s)	1676
AvaI	CYCGRG	1 cut(s)	848
AxyI	CCTNAGG	1 cut(s)	557
BaeGI	GKGCMC	3 cut(s)	1876, 2315, 2332
BalI	TGGCCA	3 cut(s)	2073, 2307, 2646
BamHI	GGATCC	1 cut(s)	2389
BanI	GGYRCC	2 cut(s)	1440, 2864
BanII	GRGCYC	4 cut(s)	984, 1518, 2215, 2315
BbsI	GAAGAC	1 cut(s)	1626
Bbv12I	GWGCWC	5 cut(s)	1246, 1876, 2215, 2690, 2956
BbvI	GCAGC	7 cut(s)	130, 706, 782, 1442, 1540, 2271, 2950
BceAI	ACGGC	6 cut(s)	684, 2082, 2552, 2690, 2934, 2985
BciT130I	CCWGG	8 cut(s)	380, 813, 1016, 2027, 2309, 2592, 2843, 2912
BciVI	GTATCC	2 cut(s)	1626, 1769
BclI	TGATCA	1 cut(s)	2227
BcnI	CCSGG	3 cut(s)	149, 318, 574
BcoDI	GTCTC	4 cut(s)	105, 659, 882, 1220
BcuI	ACTAGT	1 cut(s)	1942
BfaI	CTAG	8 cut(s)	81, 174, 1041, 1125, 1595, 1677, 1943, 2325
BfmI	CTRYAG	1 cut(s)	2869
BfoI	RGCGCY	4 cut(s)	700, 1444, 2626, 2868
BfuAI	ACCTGC	1 cut(s)	2419
BfuI	GTATCC	2 cut(s)	1626, 1769
BisI	GCNGC	8 cut(s)	78, 144, 695, 771, 1431, 1529, 2285, 2964
BlsI	GCNGC	8 cut(s)	79, 145, 696, 772, 1432, 1530, 2286, 2965
BmeRI	GACNNNNNGTC	1 cut(s)	2678
BmeT110I	CYCGRG	1 cut(s)	848
BmgBI	CACGTC	1 cut(s)	1399
BmrI	ACTGGG	4 cut(s)	1779, 2043, 2280, 3048
BmsI	GCATC	1 cut(s)	1141
BmtI	GCTAGC	1 cut(s)	1680
BmuI	ACTGGG	4 cut(s)	1779, 2043, 2280, 3048
BpiI	GAAGAC	1 cut(s)	1626
BpmI	CTGGAG	3 cut(s)	532, 2357, 2678
Bpu10I	CCTNAGC	1 cut(s)	2891
Bpu14I	TTCGAA	1 cut(s)	2401
BpuEI	CTTGAG	2 cut(s)	599, 2199
BpuMI	CCSGG	3 cut(s)	149, 318, 574
BsaBI	GATNNNNATC	1 cut(s)	2364
BsaHI	GRCGYC	2 cut(s)	1441, 2865
BsaJI	CCNNGG	8 cut(s)	147, 966, 1015, 2308, 2590, 2910, 2911, 2967
BsaWI	WCCGGW	2 cut(s)	1322, 1761
BsaXI	ACNNNNNCTCC	4 cut(s)	2202, 2232, 2240, 2270
Bsc4I	CCNNNNNNNGG	8 cut(s)	174, 762, 1043, 1266, 1322, 2065, 2080, 2632
Bse21I	CCTNAGG	1 cut(s)	557
Bse3DI	GCAATG	1 cut(s)	621
Bse8I	GATNNNNATC	1 cut(s)	2364
BseAI	TCCGGA	2 cut(s)	1322, 1761
BseBI	CCWGG	8 cut(s)	380, 813, 1016, 2027, 2309, 2592, 2843, 2912
BseDI	CCNNGG	8 cut(s)	147, 966, 1015, 2308, 2590, 2910, 2911, 2967
BseGI	GGATG	8 cut(s)	522, 976, 1558, 1715, 1984, 2365, 2749, 2793
BseJI	GATNNNNATC	1 cut(s)	2364
BseLI	CCNNNNNNNGG	8 cut(s)	174, 762, 1043, 1266, 1322, 2065, 2080, 2632
BseMI	GCAATG	1 cut(s)	621
BseMII	CTCAG	6 cut(s)	465, 525, 1958, 2373, 2556, 2817
BseRI	GAGGAG	1 cut(s)	2675
BseSI	GKGCMC	3 cut(s)	1876, 2315, 2332
BseXI	GCAGC	7 cut(s)	130, 706, 782, 1442, 1540, 2271, 2950
BseYI	CCCAGC	2 cut(s)	17, 521
BsgI	GTGCAG	1 cut(s)	569
BshFI	GGCC	7 cut(s)	305, 2073, 2307, 2313, 2646, 2949, 2972
BshNI	GGYRCC	2 cut(s)	1440, 2864
BsiHKAI	GWGCWC	5 cut(s)	1246, 1876, 2215, 2690, 2956
BsiHKCI	CYCGRG	1 cut(s)	848
BsiSI	CCGG	6 cut(s)	149, 318, 574, 1323, 1762, 2691
BslFI	GGGAC	8 cut(s)	85, 287, 610, 1006, 1282, 1718, 2623, 2893
BslI	CCNNNNNNNGG	8 cut(s)	174, 762, 1043, 1266, 1322, 2065, 2080, 2632
BsmAI	GTCTC	4 cut(s)	105, 659, 882, 1220
BsmFI	GGGAC	8 cut(s)	85, 287, 610, 1006, 1282, 1718, 2623, 2893
BsmI	GAATGC	3 cut(s)	1372, 2008, 2423
BsnI	GGCC	7 cut(s)	305, 2073, 2307, 2313, 2646, 2949, 2972
BsoBI	CYCGRG	1 cut(s)	848
Bsp119I	TTCGAA	1 cut(s)	2401
Bsp120I	GGGCCC	1 cut(s)	2311
Bsp1286I	GDGCHC	9 cut(s)	984, 1246, 1518, 1876, 2215, 2315, 2332, 2690, 2956
Bsp13I	TCCGGA	2 cut(s)	1322, 1761
Bsp1407I	TGTACA	2 cut(s)	1539, 3016
BspACI	CCGC	8 cut(s)	66, 77, 88, 1154, 1916, 2497, 2887, 3026
BspANI	GGCC	7 cut(s)	305, 2073, 2307, 2313, 2646, 2949, 2972
BspCNI	CTCAG	6 cut(s)	466, 526, 1959, 2374, 2557, 2818
BspEI	TCCGGA	2 cut(s)	1322, 1761
BspMI	ACCTGC	1 cut(s)	2419
BspOI	GCTAGC	1 cut(s)	1680
BspPI	GGATC	3 cut(s)	1307, 2384, 2397
BspQI	GCTCTTC	3 cut(s)	1251, 2712, 2841
BspT104I	TTCGAA	1 cut(s)	2401
BspT107I	GGYRCC	2 cut(s)	1440, 2864
BsrDI	GCAATG	1 cut(s)	621
BsrGI	TGTACA	2 cut(s)	1539, 3016
BssECI	CCNNGG	8 cut(s)	147, 966, 1015, 2308, 2590, 2910, 2911, 2967
BssNI	GRCGYC	2 cut(s)	1441, 2865
Bst2UI	CCWGG	8 cut(s)	380, 813, 1016, 2027, 2309, 2592, 2843, 2912
Bst4CI	ACNGT	6 cut(s)	542, 593, 738, 1338, 2095, 2989
Bst6I	CTCTTC	8 cut(s)	420, 1046, 1236, 1251, 1698, 1787, 2712, 2841
BstACI	GRCGYC	2 cut(s)	1441, 2865
BstAPI	GCANNNNNTGC	1 cut(s)	1367
BstAUI	TGTACA	2 cut(s)	1539, 3016
BstBI	TTCGAA	1 cut(s)	2401
BstC8I	GCNNGC	6 cut(s)	39, 585, 1428, 1678, 2071, 2501
BstDSI	CCRYGG	2 cut(s)	966, 2967
BstEII	GGTNACC	2 cut(s)	1952, 2585
BstENI	CCTNNNNNAGG	1 cut(s)	2630
BstF5I	GGATG	8 cut(s)	522, 976, 1558, 1715, 1984, 2365, 2749, 2793
BstH2I	RGCGCY	4 cut(s)	700, 1444, 2626, 2868
BstHHI	GCGC	4 cut(s)	699, 1443, 2625, 2867
BstMAI	GTCTC	4 cut(s)	105, 659, 882, 1220
BstNI	CCWGG	8 cut(s)	380, 813, 1016, 2027, 2309, 2592, 2843, 2912
BstPI	GGTNACC	2 cut(s)	1952, 2585
BstSFI	CTRYAG	1 cut(s)	2869
BstSLI	GKGCMC	3 cut(s)	1876, 2315, 2332
BstV1I	GCAGC	7 cut(s)	130, 706, 782, 1442, 1540, 2271, 2950
BstV2I	GAAGAC	1 cut(s)	1626
BstX2I	RGATCY	1 cut(s)	2389
BstXI	CCANNNNNNTGG	4 cut(s)	386, 1171, 1727, 2033
BstYI	RGATCY	1 cut(s)	2389
Bsu36I	CCTNAGG	1 cut(s)	557
BsuI	GTATCC	2 cut(s)	1626, 1769
BsuRI	GGCC	7 cut(s)	305, 2073, 2307, 2313, 2646, 2949, 2972
BtgI	CCRYGG	2 cut(s)	966, 2967
BtrI	CACGTC	1 cut(s)	1399
BtsCI	GGATG	8 cut(s)	522, 976, 1558, 1715, 1984, 2365, 2749, 2793
BtsI	GCAGTG	2 cut(s)	214, 2895
BtsIMutI	CAGTG	6 cut(s)	214, 471, 955, 1260, 2895, 2973
BveI	ACCTGC	1 cut(s)	2419
Cac8I	GCNNGC	6 cut(s)	39, 585, 1428, 1678, 2071, 2501
CaiI	CAGNNNCTG	1 cut(s)	995
CfoI	GCGC	4 cut(s)	699, 1443, 2625, 2867
CseI	GACGC	3 cut(s)	341, 917, 2462
CsiI	ACCWGGT	1 cut(s)	378
Csp6I	GTAC	7 cut(s)	164, 732, 1344, 1540, 1992, 3017, 3029
CspCI	CAANNNNNGTGG	2 cut(s)	948, 983
CviQI	GTAC	7 cut(s)	164, 732, 1344, 1540, 1992, 3017, 3029
DinI	GGCGCC	2 cut(s)	1442, 2866
DraI	TTTAAA	1 cut(s)	1833
DraIII	CACNNNGTG	2 cut(s)	762, 1282
DrdI	GACNNNNNNGTC	2 cut(s)	559, 1971
DriI	GACNNNNNGTC	1 cut(s)	2678
DseDI	GACNNNNNNGTC	2 cut(s)	559, 1971
EaeI	YGGCCR	5 cut(s)	2071, 2305, 2644, 2947, 2970
Eam1104I	CTCTTC	8 cut(s)	420, 1046, 1236, 1251, 1698, 1787, 2712, 2841
Eam1105I	GACNNNNNGTC	1 cut(s)	2678
EarI	CTCTTC	8 cut(s)	420, 1046, 1236, 1251, 1698, 1787, 2712, 2841
Ecl136II	GAGCTC	1 cut(s)	2213
Eco147I	AGGCCT	1 cut(s)	305
Eco24I	GRGCYC	4 cut(s)	984, 1518, 2215, 2315
Eco32I	GATATC	1 cut(s)	2816
Eco53kI	GAGCTC	1 cut(s)	2213
Eco57I	CTGAAG	3 cut(s)	212, 2333, 2828
Eco81I	CCTNAGG	1 cut(s)	557
Eco88I	CYCGRG	1 cut(s)	848
Eco91I	GGTNACC	2 cut(s)	1952, 2585
EcoICRI	GAGCTC	1 cut(s)	2213
EcoNI	CCTNNNNNAGG	1 cut(s)	2630
EcoO109I	RGGNCCY	3 cut(s)	69, 2311, 2312
EcoO65I	GGTNACC	2 cut(s)	1952, 2585
EcoRI	GAATTC	3 cut(s)	944, 1667, 2492
EcoRII	CCWGG	8 cut(s)	378, 811, 1014, 2025, 2307, 2590, 2841, 2910
EcoRV	GATATC	1 cut(s)	2816
EcoT22I	ATGCAT	1 cut(s)	2170
EcoT38I	GRGCYC	4 cut(s)	984, 1518, 2215, 2315
EgeI	GGCGCC	2 cut(s)	1442, 2866
EheI	GGCGCC	2 cut(s)	1442, 2866
FaqI	GGGAC	8 cut(s)	85, 287, 610, 1006, 1282, 1718, 2623, 2893
FauI	CCCGC	3 cut(s)	59, 81, 2894
FbaI	TGATCA	1 cut(s)	2227
FblI	GTMKAC	1 cut(s)	1221
Fnu4HI	GCNGC	8 cut(s)	78, 144, 695, 771, 1431, 1529, 2285, 2964
FokI	GGATG	8 cut(s)	529, 983, 1545, 1702, 1991, 2352, 2756, 2780
FriOI	GRGCYC	4 cut(s)	984, 1518, 2215, 2315
Fsp4HI	GCNGC	8 cut(s)	78, 144, 695, 771, 1431, 1529, 2285, 2964
FspBI	CTAG	8 cut(s)	81, 174, 1041, 1125, 1595, 1677, 1943, 2325
GlaI	GCGC	4 cut(s)	698, 1442, 2624, 2866
GluI	GCNGC	8 cut(s)	78, 144, 695, 771, 1431, 1529, 2285, 2964
GsaI	CCCAGC	2 cut(s)	21, 525
GsuI	CTGGAG	3 cut(s)	532, 2357, 2678
HaeII	RGCGCY	4 cut(s)	700, 1444, 2626, 2868
HaeIII	GGCC	7 cut(s)	305, 2073, 2307, 2313, 2646, 2949, 2972
HapII	CCGG	6 cut(s)	149, 318, 574, 1323, 1762, 2691
HgaI	GACGC	3 cut(s)	341, 917, 2462
HhaI	GCGC	4 cut(s)	699, 1443, 2625, 2867
Hin1I	GRCGYC	2 cut(s)	1441, 2865
Hin6I	GCGC	4 cut(s)	697, 1441, 2623, 2865
HinP1I	GCGC	4 cut(s)	697, 1441, 2623, 2865
HinfI	GANTC	9 cut(s)	266, 667, 1056, 1183, 1228, 1555, 1598, 2158, 2249
HpaII	CCGG	6 cut(s)	149, 318, 574, 1323, 1762, 2691
HphI	GGTGA	7 cut(s)	1004, 1010, 1297, 1731, 1904, 2207, 2579
Hpy99I	CGWCG	3 cut(s)	1491, 2478, 2933
HpyCH4III	ACNGT	6 cut(s)	542, 593, 738, 1338, 2095, 2989
HpyCH4IV	ACGT	3 cut(s)	1398, 1870, 2439
HpySE526I	ACGT	3 cut(s)	1398, 1870, 2439
Hsp92I	GRCGYC	2 cut(s)	1441, 2865
HspAI	GCGC	4 cut(s)	697, 1441, 2623, 2865
KasI	GGCGCC	2 cut(s)	1440, 2864
Kpn2I	TCCGGA	2 cut(s)	1322, 1761
Ksp22I	TGATCA	1 cut(s)	2227
LguI	GCTCTTC	3 cut(s)	1251, 2712, 2841
LmnI	GCTCC	6 cut(s)	2210, 2338, 2524, 2693, 2959, 3037
Lsp1109I	GCAGC	7 cut(s)	130, 706, 782, 1442, 1540, 2271, 2950
LweI	GCATC	1 cut(s)	1141
MabI	ACCWGGT	1 cut(s)	378
MaeI	CTAG	8 cut(s)	81, 174, 1041, 1125, 1595, 1677, 1943, 2325
MaeII	ACGT	3 cut(s)	1398, 1870, 2439
MfeI	CAATTG	2 cut(s)	2422, 3042
MflI	RGATCY	1 cut(s)	2389
MhlI	GDGCHC	9 cut(s)	984, 1246, 1518, 1876, 2215, 2315, 2332, 2690, 2956
MlsI	TGGCCA	3 cut(s)	2073, 2307, 2646
MluNI	TGGCCA	3 cut(s)	2073, 2307, 2646
Mly113I	GGCGCC	2 cut(s)	1441, 2865
MlyI	GAGTC	6 cut(s)	275, 661, 1222, 1607, 2167, 2258
MmeI	TCCRAC	1 cut(s)	1096
Mox20I	TGGCCA	3 cut(s)	2073, 2307, 2646
Mph1103I	ATGCAT	1 cut(s)	2170
MroI	TCCGGA	2 cut(s)	1322, 1761
MscI	TGGCCA	3 cut(s)	2073, 2307, 2646
MseI	TTAA	4 cut(s)	1647, 1734, 1832, 2619
MslI	CAYNNNNRTG	8 cut(s)	1191, 1239, 1283, 1386, 1419, 1458, 2325, 2900
Msp20I	TGGCCA	3 cut(s)	2073, 2307, 2646
MspA1I	CMGCKG	2 cut(s)	1433, 2963
MspI	CCGG	6 cut(s)	149, 318, 574, 1323, 1762, 2691
MunI	CAATTG	2 cut(s)	2422, 3042
Mva1269I	GAATGC	3 cut(s)	1372, 2008, 2423
MvaI	CCWGG	8 cut(s)	380, 813, 1016, 2027, 2309, 2592, 2843, 2912
NarI	GGCGCC	2 cut(s)	1441, 2865
NciI	CCSGG	3 cut(s)	149, 318, 574
NheI	GCTAGC	1 cut(s)	1676
NmeAIII	GCCGAG	1 cut(s)	134
NmuCI	GTSAC	7 cut(s)	389, 929, 1085, 1332, 1737, 2440, 2585
NsiI	ATGCAT	1 cut(s)	2170
NspV	TTCGAA	1 cut(s)	2401
PasI	CCCWGGG	1 cut(s)	2911
PceI	AGGCCT	1 cut(s)	305
PciSI	GCTCTTC	3 cut(s)	1251, 2712, 2841
PctI	GAATGC	3 cut(s)	1372, 2008, 2423
PfeI	GAWTC	3 cut(s)	1056, 1183, 1555
PflMI	CCANNNNNTGG	1 cut(s)	762
PfoI	TCCNGGA	1 cut(s)	2841
PkrI	GCNGC	8 cut(s)	79, 145, 696, 772, 1432, 1530, 2286, 2965
PleI	GAGTC	6 cut(s)	274, 661, 1222, 1606, 2166, 2257
PluTI	GGCGCC	2 cut(s)	1444, 2868
PpsI	GAGTC	6 cut(s)	274, 661, 1222, 1606, 2166, 2257
PpuMI	RGGWCCY	1 cut(s)	69
Psp124BI	GAGCTC	1 cut(s)	2215
Psp5II	RGGWCCY	1 cut(s)	69
Psp6I	CCWGG	8 cut(s)	378, 811, 1014, 2025, 2307, 2590, 2841, 2910
PspEI	GGTNACC	2 cut(s)	1952, 2585
PspFI	CCCAGC	2 cut(s)	17, 521
PspGI	CCWGG	8 cut(s)	378, 811, 1014, 2025, 2307, 2590, 2841, 2910
PspOMI	GGGCCC	1 cut(s)	2311
PspPPI	RGGWCCY	1 cut(s)	69
PstNI	CAGNNNCTG	1 cut(s)	995
PsuI	RGATCY	1 cut(s)	2389
PvuII	CAGCTG	2 cut(s)	1433, 2963
RsaI	GTAC	7 cut(s)	165, 733, 1345, 1541, 1993, 3018, 3030
RsaNI	GTAC	7 cut(s)	164, 732, 1344, 1540, 1992, 3017, 3029
RseI	CAYNNNNRTG	8 cut(s)	1191, 1239, 1283, 1386, 1419, 1458, 2325, 2900
SacI	GAGCTC	1 cut(s)	2215
SapI	GCTCTTC	3 cut(s)	1251, 2712, 2841
SaqAI	TTAA	4 cut(s)	1647, 1734, 1832, 2619
SatI	GCNGC	8 cut(s)	78, 144, 695, 771, 1431, 1529, 2285, 2964
SchI	GAGTC	6 cut(s)	275, 661, 1222, 1607, 2167, 2258
SduI	GDGCHC	9 cut(s)	984, 1246, 1518, 1876, 2215, 2315, 2332, 2690, 2956
SexAI	ACCWGGT	1 cut(s)	378
SfaNI	GCATC	1 cut(s)	1141
SfcI	CTRYAG	1 cut(s)	2869
SfoI	GGCGCC	2 cut(s)	1442, 2866
SfuI	TTCGAA	1 cut(s)	2401
SmiMI	CAYNNNNRTG	8 cut(s)	1191, 1239, 1283, 1386, 1419, 1458, 2325, 2900
SmlI	CTYRAG	2 cut(s)	578, 2214
SmoI	CTYRAG	2 cut(s)	578, 2214
SpeI	ACTAGT	1 cut(s)	1942
SseBI	AGGCCT	1 cut(s)	305
SsiI	CCGC	8 cut(s)	66, 77, 88, 1154, 1916, 2497, 2887, 3026
SspDI	GGCGCC	2 cut(s)	1440, 2864
SspI	AATATT	1 cut(s)	1139
SspMI	CTAG	8 cut(s)	81, 174, 1041, 1125, 1595, 1677, 1943, 2325
SstI	GAGCTC	1 cut(s)	2215
StuI	AGGCCT	1 cut(s)	305
TaaI	ACNGT	6 cut(s)	542, 593, 738, 1338, 2095, 2989
TaiI	ACGT	3 cut(s)	1401, 1873, 2442
TaqI	TCGA	8 cut(s)	59, 486, 1033, 1489, 1963, 2150, 2401, 2928
TatI	WGTACW	4 cut(s)	731, 1343, 1539, 3016
TauI	GCSGC	1 cut(s)	80
TfiI	GAWTC	3 cut(s)	1056, 1183, 1555
Tru1I	TTAA	4 cut(s)	1647, 1734, 1832, 2619
Tru9I	TTAA	4 cut(s)	1647, 1734, 1832, 2619
TscAI	CASTG	6 cut(s)	214, 478, 955, 1267, 2902, 2980
TseFI	GTSAC	7 cut(s)	389, 929, 1085, 1332, 1737, 2440, 2585
TseI	GCWGC	7 cut(s)	143, 694, 770, 1430, 1528, 2284, 2963
Tsp45I	GTSAC	7 cut(s)	389, 929, 1085, 1332, 1737, 2440, 2585
TspGWI	ACGGA	2 cut(s)	87, 2223
TspRI	CASTG	6 cut(s)	214, 478, 955, 1267, 2902, 2980
Van91I	CCANNNNNTGG	1 cut(s)	762
VneI	GTGCAC	1 cut(s)	1872
XagI	CCTNNNNNAGG	1 cut(s)	2630
XapI	RAATTY	7 cut(s)	289, 321, 944, 1653, 1667, 2409, 2492
XbaI	TCTAGA	1 cut(s)	1594
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2066, 2908
XmiI	GTMKAC	1 cut(s)	1221
XspI	CTAG	8 cut(s)	81, 174, 1041, 1125, 1595, 1677, 1943, 2325
Zsp2I	ATGCAT	1 cut(s)	2170

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.