Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1C

Orthogroup ID

OG0001811

Physical Location & Seq

Genomic Coordinates Forward (+)

58605340 .. 58611755

Gene Structure 6416 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1CG312100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2772 bp

ATGAGGTGGTTTAGAGCAGGGTCTAGTGTGGCAAAGTTTGCCATTAGGAGATCACTATCGAATAGGCATGTAGTTCCTTTGCAAAATCGATACTTGCAAACCACAATGTGCATGTATTCCAAGGCAGGGGCTGCCCCGGTTCCTCGGCCGGTGCCGCTTTCGAAGCTGACTGATAGTTTCTTGGATGGAAGCAATAGTGTTTATTTAGAGGGGCTTCAGAGGGCCTGGGAGGCTGATCCCAGCAGTGTTGATGAATCCTGGGATAATTTCTTTAGGAATTTTGTGGGGCAGGCCTCTACCTCCCCTGGAGTTTCGGGGCAGACCATTCAAGAGAGTATGCGTTTGTTGTTGCTTGTGAGGGCTTATCAGGTTACAGGTCATATGAAAGCTAAATTGGATCCTTTGGCATTGCAGGAGAGGAAAGTCCCAGATGAGTTGGACCCTGCTTCATATGGGTTTACTGAGGCTGATCTTGATAGGGAGTTCTTTTTAGGGGTTTGGAGGATAGCTGGGTTTCTGGCTGAGAATCGTCCGGTGCAGACCCTTAGGTCCATATTGACCCGGCTAGAGCAGGCTTACTGTGGGAGCATTGGGTATGAGTATATGCACATTGCGGATCGTAACAAATGTAACTGGTTGAGAGAAAAGATTGAGACTCCAACAATGATGCAATACAATGGGCAGCGCCGTGAGGTCTTTCTTGACAGGCTTATTTGGAGTACACAGTTTGAGAACTTCTTGCACTCTAAGTGGAAAACAGCAAAGAGGTTTGGGCTTGAAGGTTGCGAAACTCTCATTCCTGGCATGAAGGAGATGTTTGATAGGGCAGCAGATCTTGGGGTTGAGAGCATAGTCATTGGAATGCCTCATAGAGGAAGACTGAATGTATTGGGTAATGTGGTCCGAAAACCTTTGCGGCAGATATTCAGTGAATTCAGTGGTGGTACAAAGCCTGTGGATGAAGATGGGCTGTACTCAGGGACTGGTGATGTCAAGTATCACTTGGGAACTTCCTATGATCGACCCACTAGAGGTGGGAAAAGACTTCATCTATCTTTGGTTGCAAATCCAAGTCACTTGGAAGCTGTGGATCCAGTTGTTGTTGGAAAAACAAGAGCAAAGCAATTTTATTCAAATGATTTGGACAGGACCAAGAATATGGCAGTTTTGATTCATGGAGATGGTAGCTTTGCCGGACAAGGAGTGGTCTACGAGACTCTGCATCTTAGTGCTCTTCCTAATTACACCACTGGTGGGACTATACACATTGTAGTGAATAACCAAGTTGCCTTCACAACTGATCCAATGTCCGGCAGATCTTCAGAGTATTGCACTGATGTTGCTAAAGCCTTGAATGTCCCTATTTTCCATGTAAATGCTGATGACATGGAGGCAGTTGTTCATGTCTGTGAGCTTGCAGCTGAGTGGCGCCAGACATTCCATTCTGATGTTGTAGTTGATTTAGTGTGTTATCGTCGATTTGGGCATAATGAGTTAGATGAGCCATCATTTACGCAGCCTAAAATGTACCAGGTTATTAAAAATCATCCGAACCCTCTAACTATTTACACGAACAAACTTTTAGAATCTGGACAGGTGAACCAAGAAGATATTGAGAGGATACAGAGCAAGGTTAATAAGATTCTAAACGAAGAGTTCTTAGCTAGCAAAGATTATGTTCCTCAAAGAAGGGATTGGCTTGCATCTCATTGGACTGGATTCAAGTCACCTGAACAACTTTCTCGTATCCGGAACACTGGGGTAAAACCAGAGATTTTGAAGGATGTTGGCAAAGCAATTACATTTTTACCAGAAAATTTTAAACCTCATAGAGCAGTAAAGAAGGTATATGGAGAACGTGCAGAAATGATTGAGACTGGTGAAGGTATTGATTGGGCAGTTGCAGAAGCACTTGCCTTTGCTACACTGCTTGTTGAAGGTAACCATGTTCGACTGAGTGGTCAGGATGTTGAGAGAGGTACATTTAGTCATCGGCATTCTGTACTTCATGACCAGGAAACAGGGGAAAAGTATTGCCCTCTGGACCATGTTATGGCGAACCAAGATGAGGAATTGTTTACAGTTAGCAACAGCTCACTTTCGGAGTTCGGTGTTCTTGGATTTGAATTGGGTTACTCGATGGAGAATCCAAATGCGTTGGTACTTTGGGAAGCTCAATTTGGTGATTTTGCCAATGGAGCTCAAGTTATATTTGATCAGTTTTTGAGTAGTGGGGAGTCAAAGTGGCTTCGTCAAACTGGGCTTGTTGTACTGCTTCCACACAGTTATGCTGGTCAGGGACCTGAACATTCAAGTGCCCGATTGGAGCGATTTCTTCAGATGAGTGATGACAATCCTTACCTCATACCTGAGATGGATCATACACATCGGAAGCAAATTCAGGAATGTAATTGGCAGGTTGTGAATGTGACAACTCCTGCTAATTATTTCCATGTCCTGCGTCGTCAGATAAACAGGGAGTTCCGCAAGCCTCTTGTTGTGATGGCTCCTAAAAACTTGCTTCGTCACAAAGACTGCAAATCAAATTTATCTGAGTTTGATGATGTTGAAGGCCATCCAGGTTTTGACAAGCAGGGGACCAGATTTAAGCGCCTTATAAAGGACCAGAATGATCACTCTGATCTGGAGGAGGGTATTAGACGGCTTGTTCTTTGCTCCGGAAAGGTATTGTGTCCCTGCATGTGTACCGTATTTTCATTGGTATGCATCATGTTGGCTTCTGTTGTATACATCATATTGACTTCTAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004591 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005947 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0008150 GO:0008152 GO:0009060 GO:0009353 GO:0009987 GO:0015980 GO:0016491 GO:0016624 GO:0016903 GO:0016999 GO:0017144 GO:0019752 GO:0030062 GO:0031974 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045239 GO:0045240 GO:0045252 GO:0045333 GO:0055114 GO:0070013 GO:0071704 GO:0072350 GO:0098798 GO:1902494 GO:1990204 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00020 ko00310 ko00380 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map00380 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

2-oxogl_dehyd_N E1_dh OxoGdeHyase_C Transket_pyr

Protein Analysis

923

Amino Acids

104.58

Weight (kDa)

6.54

Isoelectric Point (pI)

38.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-oxogl_dehyd_N	PF16078	58 - 95	1.3e-14	2-oxoglutarate dehydrogenase N-terminus
E1_dh	PF00676	239 - 559	6e-62	Dehydrogenase E1 component
Transket_pyr	PF02779	629 - 842	1.7e-48	Transketolase, pyrimidine binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001811)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55410 AT3G55410 AT5G65750
fragaria_vesca	FvH4_7g21770 FvH4_7g21770 FvH4_7g21770 FvH4_7g21770 FvH4_7g21770 FvH4_7g21770 FvH4_7g21851
malus_domestica	MD01G1122800.v1.1 MD02G1281300.v1.1 MD02G1281500.v1.1 MD07G1192300.v1.1
prunus_persica	Prupe.2G230300_v2.0.a1 Prupe.2G230400_v2.0.a1 Prupe.2G230400_v2.0.a1 Prupe.2G230400_v2.0.a1
pyrus_communis	pycom01g14980 pycom02g24050 pycom07g18180
rosa_chinensis	RchiOBHm_Chr1g0365921 RchiOBHm_Chr1g0366031
rosa_laevigata	RLG00000027377 RLG00000027378
rosa_multiflora	Rmu_co8265029.1_g000001 Rmu_sc0004288.1_g000005 Rmu_sc0006406.1_g000008
rosa_roxburghii	Rroxscaffold_4G00290620 Rroxscaffold_4G00290640
rosa_rugosa	Rorug01G0325800 Rorug01G0325900 Rorug01G0326000 Rorug01G0326100 Rorug01G0326200 Rorug01G0326300 Rorug01G0326400
rosa_samantha	Rh1AG334500 Rh1AG334600 Rh1BG295400 Rh1BG295600 Rh1CG312100 Rh1CG312200 Rh1DG327700 Rh1DG328000
rosa_wichuraiana	Rw1G029690 Rw1G029720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2618
AasI	GACNNNNNNGTC	2 cut(s)	547, 1959
Acc36I	ACCTGC	1 cut(s)	2407
AccB1I	GGYRCC	2 cut(s)	151, 1428
AccB7I	CCANNNNNTGG	1 cut(s)	2053
AccI	GTMKAC	2 cut(s)	1209, 2748
AccIII	TCCGGA	2 cut(s)	1749, 2678
AciI	CCGC	4 cut(s)	155, 614, 916, 2485
AclWI	GGATC	8 cut(s)	230, 392, 405, 624, 1085, 1098, 1295, 2385
AcoI	YGGCCR	1 cut(s)	146
AcsI	RAATTY	5 cut(s)	277, 932, 1816, 2397, 2545
AcuI	CTGAAG	3 cut(s)	200, 1305, 2321
AcyI	GRCGYC	1 cut(s)	1429
AdeI	CACNNNGTG	1 cut(s)	108
AfaI	GTAC	9 cut(s)	721, 946, 974, 1529, 1981, 2004, 2163, 2271, 2707
AfiI	CCNNNNNNNGG	8 cut(s)	126, 872, 1031, 1254, 1310, 2053, 2068, 2620
AjnI	CCWGG	7 cut(s)	224, 257, 304, 799, 1530, 2013, 2578
AjuI	GAANNNNNNNTTGG	4 cut(s)	377, 409, 2163, 2195
Alw21I	GWGCWC	2 cut(s)	1234, 2203
Alw26I	GTCTC	3 cut(s)	647, 1208, 1868
AlwI	GGATC	8 cut(s)	230, 392, 405, 624, 1085, 1098, 1295, 2385
AlwNI	CAGNNNCTG	2 cut(s)	131, 983
Aor13HI	TCCGGA	2 cut(s)	1749, 2678
AoxI	GGCC	4 cut(s)	146, 222, 291, 2572
ApeKI	GCWGC	5 cut(s)	131, 682, 827, 1418, 1516
ApoI	RAATTY	5 cut(s)	277, 932, 1816, 2397, 2545
AspLEI	GCGC	3 cut(s)	687, 1431, 2613
AspS9I	GGNCC	9 cut(s)	222, 439, 549, 901, 1149, 2044, 2300, 2598, 2623
AsuC2I	CCSGG	2 cut(s)	137, 562
AsuHPI	GGTGA	5 cut(s)	998, 1609, 1719, 1892, 2195
AsuII	TTCGAA	1 cut(s)	161
AsuNHI	GCTAGC	1 cut(s)	1664
AvaII	GGWCC	8 cut(s)	439, 549, 901, 1149, 2044, 2300, 2598, 2623
AxyI	CCTNAGG	1 cut(s)	545
BaeGI	GKGCMC	1 cut(s)	2320
BamHI	GGATCC	2 cut(s)	397, 1090
BanI	GGYRCC	2 cut(s)	151, 1428
BanII	GRGCYC	1 cut(s)	2203
BbsI	GAAGAC	1 cut(s)	883
Bbv12I	GWGCWC	2 cut(s)	1234, 2203
BbvI	GCAGC	5 cut(s)	118, 694, 839, 1430, 1528
BccI	CCATC	8 cut(s)	179, 959, 1175, 1513, 2134, 2368, 2497, 2583
BceAI	ACGGC	2 cut(s)	672, 2678
BciT130I	CCWGG	7 cut(s)	226, 259, 306, 801, 1532, 2015, 2580
BciVI	GTATCC	2 cut(s)	1614, 1757
BclI	TGATCA	2 cut(s)	2215, 2632
BcnI	CCSGG	2 cut(s)	137, 562
BcoDI	GTCTC	3 cut(s)	647, 1208, 1868
BfaI	CTAG	5 cut(s)	24, 566, 1029, 1665, 2766
BfoI	RGCGCY	3 cut(s)	688, 1432, 2614
BfuAI	ACCTGC	1 cut(s)	2407
BfuI	GTATCC	2 cut(s)	1614, 1757
BglI	GCCNNNNNGGC	1 cut(s)	230
BglII	AGATCT	2 cut(s)	832, 1316
BisI	GCNGC	7 cut(s)	132, 155, 683, 828, 917, 1419, 1517
BlsI	GCNGC	7 cut(s)	133, 156, 684, 829, 918, 1420, 1518
Bme1390I	CCNGG	9 cut(s)	137, 226, 259, 306, 562, 801, 1532, 2015, 2580
Bme18I	GGWCC	8 cut(s)	439, 549, 901, 1149, 2044, 2300, 2598, 2623
BmgT120I	GGNCC	9 cut(s)	222, 439, 549, 901, 1149, 2044, 2300, 2598, 2623
BmiI	GGNNCC	9 cut(s)	141, 153, 399, 441, 1092, 1430, 2301, 2508, 2599
BmrFI	CCNGG	9 cut(s)	137, 226, 259, 306, 562, 801, 1532, 2015, 2580
BmrI	ACTGGG	2 cut(s)	1767, 2268
BmsI	GCATC	4 cut(s)	657, 1231, 1712, 2736
BmtI	GCTAGC	1 cut(s)	1668
BmuI	ACTGGG	2 cut(s)	1767, 2268
BpiI	GAAGAC	1 cut(s)	883
BpmI	CTGGAG	2 cut(s)	327, 2666
Bpu14I	TTCGAA	1 cut(s)	161
BpuEI	CTTGAG	1 cut(s)	2187
BpuMI	CCSGG	2 cut(s)	137, 562
Bsa29I	ATCGAT	1 cut(s)	88
BsaBI	GATNNNNATC	2 cut(s)	55, 2352
BsaHI	GRCGYC	1 cut(s)	1429
BsaJI	CCNNGG	6 cut(s)	120, 135, 143, 225, 258, 304
BsaWI	WCCGGW	3 cut(s)	532, 1749, 2678
BsaXI	ACNNNNNCTCC	4 cut(s)	2190, 2220, 2228, 2258
Bsc4I	CCNNNNNNNGG	8 cut(s)	126, 872, 1031, 1254, 1310, 2053, 2068, 2620
Bse118I	RCCGGY	1 cut(s)	148
Bse1I	ACTGG	8 cut(s)	638, 988, 1094, 1255, 1720, 1762, 1882, 2263
Bse21I	CCTNAGG	1 cut(s)	545
Bse3DI	GCAATG	2 cut(s)	407, 609
Bse8I	GATNNNNATC	2 cut(s)	55, 2352
BseAI	TCCGGA	2 cut(s)	1749, 2678
BseBI	CCWGG	7 cut(s)	226, 259, 306, 801, 1532, 2015, 2580
BseCI	ATCGAT	1 cut(s)	88
BseDI	CCNNGG	6 cut(s)	120, 135, 143, 225, 258, 304
BseGI	GGATG	6 cut(s)	190, 964, 1546, 1789, 1972, 2575
BseJI	GATNNNNATC	2 cut(s)	55, 2352
BseLI	CCNNNNNNNGG	8 cut(s)	126, 872, 1031, 1254, 1310, 2053, 2068, 2620
BseMI	GCAATG	2 cut(s)	407, 609
BseMII	CTCAG	7 cut(s)	453, 513, 990, 1413, 1946, 2361, 2544
BseNI	ACTGG	8 cut(s)	638, 988, 1094, 1255, 1720, 1762, 1882, 2263
BseRI	GAGGAG	1 cut(s)	2663
BseSI	GKGCMC	1 cut(s)	2320
BseX3I	CGGCCG	1 cut(s)	146
BseXI	GCAGC	5 cut(s)	118, 694, 839, 1430, 1528
BseYI	CCCAGC	2 cut(s)	239, 509
BsgI	GTGCAG	2 cut(s)	557, 1881
Bsh1285I	CGRYCG	1 cut(s)	149
BshFI	GGCC	4 cut(s)	148, 224, 293, 2574
BshNI	GGYRCC	2 cut(s)	151, 1428
BshVI	ATCGAT	1 cut(s)	88
BsiEI	CGRYCG	1 cut(s)	149
BsiHKAI	GWGCWC	2 cut(s)	1234, 2203
BsiSI	CCGG	8 cut(s)	137, 149, 533, 562, 1194, 1311, 1750, 2679
BslFI	GGGAC	7 cut(s)	410, 994, 1270, 1343, 2313, 2611, 2679
BslI	CCNNNNNNNGG	8 cut(s)	126, 872, 1031, 1254, 1310, 2053, 2068, 2620
BsmAI	GTCTC	3 cut(s)	647, 1208, 1868
BsmFI	GGGAC	7 cut(s)	410, 994, 1270, 1343, 2313, 2611, 2679
BsmI	GAATGC	2 cut(s)	867, 1996
BsnI	GGCC	4 cut(s)	148, 224, 293, 2574
Bsp119I	TTCGAA	1 cut(s)	161
Bsp1286I	GDGCHC	3 cut(s)	1234, 2203, 2320
Bsp13I	TCCGGA	2 cut(s)	1749, 2678
BspACI	CCGC	4 cut(s)	155, 614, 916, 2485
BspANI	GGCC	4 cut(s)	148, 224, 293, 2574
BspCNI	CTCAG	7 cut(s)	454, 514, 989, 1414, 1947, 2362, 2545
BspDI	ATCGAT	1 cut(s)	88
BspEI	TCCGGA	2 cut(s)	1749, 2678
BspHI	TCATGA	1 cut(s)	2008
BspLI	GGNNCC	9 cut(s)	141, 153, 399, 441, 1092, 1430, 2301, 2508, 2599
BspMI	ACCTGC	1 cut(s)	2407
BspOI	GCTAGC	1 cut(s)	1668
BspPI	GGATC	8 cut(s)	230, 392, 405, 624, 1085, 1098, 1295, 2385
BspQI	GCTCTTC	1 cut(s)	1239
BspT104I	TTCGAA	1 cut(s)	161
BspT107I	GGYRCC	2 cut(s)	151, 1428
BsrDI	GCAATG	2 cut(s)	407, 609
BsrFI	RCCGGY	1 cut(s)	148
BsrI	ACTGG	8 cut(s)	638, 988, 1094, 1255, 1720, 1762, 1882, 2263
BssAI	RCCGGY	1 cut(s)	148
BssECI	CCNNGG	6 cut(s)	120, 135, 143, 225, 258, 304
BssNAI	GTATAC	1 cut(s)	2749
BssNI	GRCGYC	1 cut(s)	1429
BssT1I	CCWWGG	1 cut(s)	120
Bst1107I	GTATAC	1 cut(s)	2749
Bst2UI	CCWGG	7 cut(s)	226, 259, 306, 801, 1532, 2015, 2580
Bst4CI	ACNGT	5 cut(s)	581, 726, 2083, 2285, 2710
Bst6I	CTCTTC	2 cut(s)	1239, 1647
BstACI	GRCGYC	1 cut(s)	1429
BstAPI	GCANNNNNTGC	2 cut(s)	38, 131
BstBI	TTCGAA	1 cut(s)	161
BstC8I	GCNNGC	6 cut(s)	291, 573, 1416, 1666, 1701, 2489
BstEII	GGTNACC	1 cut(s)	1940
BstENI	CCTNNNNNAGG	2 cut(s)	870, 2618
BstF5I	GGATG	6 cut(s)	190, 964, 1546, 1789, 1972, 2575
BstH2I	RGCGCY	3 cut(s)	688, 1432, 2614
BstHHI	GCGC	3 cut(s)	687, 1431, 2613
BstMAI	GTCTC	3 cut(s)	647, 1208, 1868
BstMCI	CGRYCG	1 cut(s)	149
BstMWI	GCNNNNNNNGC	6 cut(s)	38, 131, 154, 163, 230, 2198
BstNI	CCWGG	7 cut(s)	226, 259, 306, 801, 1532, 2015, 2580
BstNSI	RCATGY	3 cut(s)	71, 115, 2704
BstPI	GGTNACC	1 cut(s)	1940
BstSCI	CCNGG	9 cut(s)	135, 224, 257, 304, 560, 799, 1530, 2013, 2578
BstSLI	GKGCMC	1 cut(s)	2320
BstV1I	GCAGC	5 cut(s)	118, 694, 839, 1430, 1528
BstV2I	GAAGAC	1 cut(s)	883
BstX2I	RGATCY	4 cut(s)	397, 832, 1090, 1316
BstXI	CCANNNNNNTGG	1 cut(s)	1159
BstYI	RGATCY	4 cut(s)	397, 832, 1090, 1316
BstZ17I	GTATAC	1 cut(s)	2749
BstZI	CGGCCG	1 cut(s)	146
Bsu15I	ATCGAT	1 cut(s)	88
Bsu36I	CCTNAGG	1 cut(s)	545
BsuI	GTATCC	2 cut(s)	1614, 1757
BsuRI	GGCC	4 cut(s)	148, 224, 293, 2574
BsuTUI	ATCGAT	1 cut(s)	88
BtsCI	GGATG	6 cut(s)	190, 964, 1546, 1789, 1972, 2575
BtsI	GCAGTG	2 cut(s)	250, 1925
BtsIMutI	CAGTG	7 cut(s)	250, 934, 943, 1248, 1332, 1755, 1925
BveI	ACCTGC	1 cut(s)	2407
Cac8I	GCNNGC	6 cut(s)	291, 573, 1416, 1666, 1701, 2489
CaiI	CAGNNNCTG	2 cut(s)	131, 983
CciI	TCATGA	1 cut(s)	2008
CfoI	GCGC	3 cut(s)	687, 1431, 2613
Cfr10I	RCCGGY	1 cut(s)	148
Cfr13I	GGNCC	9 cut(s)	222, 439, 549, 901, 1149, 2044, 2300, 2598, 2623
ClaI	ATCGAT	1 cut(s)	88
CseI	GACGC	1 cut(s)	2450
CsiI	ACCWGGT	1 cut(s)	1530
Csp6I	GTAC	9 cut(s)	720, 945, 973, 1528, 1980, 2003, 2162, 2270, 2706
CspCI	CAANNNNNGTGG	2 cut(s)	936, 971
CviQI	GTAC	9 cut(s)	720, 945, 973, 1528, 1980, 2003, 2162, 2270, 2706
DinI	GGCGCC	1 cut(s)	1430
DraI	TTTAAA	1 cut(s)	1822
DraIII	CACNNNGTG	1 cut(s)	108
DrdI	GACNNNNNNGTC	2 cut(s)	547, 1959
DseDI	GACNNNNNNGTC	2 cut(s)	547, 1959
EaeI	YGGCCR	1 cut(s)	146
EagI	CGGCCG	1 cut(s)	146
Eam1104I	CTCTTC	2 cut(s)	1239, 1647
EarI	CTCTTC	2 cut(s)	1239, 1647
Ecl136II	GAGCTC	1 cut(s)	2201
EclXI	CGGCCG	1 cut(s)	146
Eco130I	CCWWGG	1 cut(s)	120
Eco147I	AGGCCT	1 cut(s)	293
Eco24I	GRGCYC	1 cut(s)	2203
Eco47I	GGWCC	8 cut(s)	439, 549, 901, 1149, 2044, 2300, 2598, 2623
Eco52I	CGGCCG	1 cut(s)	146
Eco53kI	GAGCTC	1 cut(s)	2201
Eco57I	CTGAAG	3 cut(s)	200, 1305, 2321
Eco81I	CCTNAGG	1 cut(s)	545
Eco91I	GGTNACC	1 cut(s)	1940
EcoICRI	GAGCTC	1 cut(s)	2201
EcoNI	CCTNNNNNAGG	2 cut(s)	870, 2618
EcoO109I	RGGNCCY	2 cut(s)	222, 2300
EcoO65I	GGTNACC	1 cut(s)	1940
EcoRI	GAATTC	1 cut(s)	932
EcoRII	CCWGG	7 cut(s)	224, 257, 304, 799, 1530, 2013, 2578
EcoT14I	CCWWGG	1 cut(s)	120
EcoT22I	ATGCAT	1 cut(s)	2729
EcoT38I	GRGCYC	1 cut(s)	2203
EgeI	GGCGCC	1 cut(s)	1430
EheI	GGCGCC	1 cut(s)	1430
ErhI	CCWWGG	1 cut(s)	120
FalI	AAGNNNNNCTT	2 cut(s)	986, 1018
FaqI	GGGAC	7 cut(s)	410, 994, 1270, 1343, 2313, 2611, 2679
FauNDI	CATATG	2 cut(s)	381, 451
FbaI	TGATCA	2 cut(s)	2215, 2632
FblI	GTMKAC	2 cut(s)	1209, 2748
Fnu4HI	GCNGC	7 cut(s)	132, 155, 683, 828, 917, 1419, 1517
FokI	GGATG	6 cut(s)	197, 971, 1533, 1796, 1979, 2562
FriOI	GRGCYC	1 cut(s)	2203
Fsp4HI	GCNGC	7 cut(s)	132, 155, 683, 828, 917, 1419, 1517
FspBI	CTAG	5 cut(s)	24, 566, 1029, 1665, 2766
GlaI	GCGC	3 cut(s)	686, 1430, 2612
GluI	GCNGC	7 cut(s)	132, 155, 683, 828, 917, 1419, 1517
GsaI	CCCAGC	2 cut(s)	243, 513
GsuI	CTGGAG	2 cut(s)	327, 2666
HaeII	RGCGCY	3 cut(s)	688, 1432, 2614
HaeIII	GGCC	4 cut(s)	148, 224, 293, 2574
HapII	CCGG	8 cut(s)	137, 149, 533, 562, 1194, 1311, 1750, 2679
HgaI	GACGC	1 cut(s)	2450
HhaI	GCGC	3 cut(s)	687, 1431, 2613
Hin1I	GRCGYC	1 cut(s)	1429
Hin6I	GCGC	3 cut(s)	685, 1429, 2611
HinP1I	GCGC	3 cut(s)	685, 1429, 2611
HpaII	CCGG	8 cut(s)	137, 149, 533, 562, 1194, 1311, 1750, 2679
HphI	GGTGA	5 cut(s)	998, 1609, 1719, 1892, 2195
Hpy166II	GTNNAC	7 cut(s)	459, 722, 1210, 1600, 2079, 2706, 2749
Hpy8I	GTNNAC	7 cut(s)	459, 722, 1210, 1600, 2079, 2706, 2749
Hpy99I	CGWCG	2 cut(s)	1479, 2466
HpyAV	CCTTC	9 cut(s)	773, 802, 1300, 1683, 1774, 1837, 1877, 1931, 2564
HpyCH4III	ACNGT	5 cut(s)	581, 726, 2083, 2285, 2710
HpyCH4IV	ACGT	1 cut(s)	1858
HpyF10VI	GCNNNNNNNGC	6 cut(s)	38, 131, 154, 163, 230, 2198
HpySE526I	ACGT	1 cut(s)	1858
Hsp92I	GRCGYC	1 cut(s)	1429
HspAI	GCGC	3 cut(s)	685, 1429, 2611
KasI	GGCGCC	1 cut(s)	1428
Kpn2I	TCCGGA	2 cut(s)	1749, 2678
Ksp22I	TGATCA	2 cut(s)	2215, 2632
LguI	GCTCTTC	1 cut(s)	1239
LmnI	GCTCC	5 cut(s)	585, 2198, 2326, 2512, 2681
Lsp1109I	GCAGC	5 cut(s)	118, 694, 839, 1430, 1528
LweI	GCATC	4 cut(s)	657, 1231, 1712, 2736
MabI	ACCWGGT	1 cut(s)	1530
MaeI	CTAG	5 cut(s)	24, 566, 1029, 1665, 2766
MaeII	ACGT	1 cut(s)	1858
MaeIII	GTNAC	9 cut(s)	370, 620, 629, 1073, 1725, 1940, 2132, 2428, 2525
MboII	GAAGA	7 cut(s)	888, 974, 1226, 1311, 1619, 1664, 2327
MflI	RGATCY	4 cut(s)	397, 832, 1090, 1316
MhlI	GDGCHC	3 cut(s)	1234, 2203, 2320
Mly113I	GGCGCC	1 cut(s)	1429
MlyI	GAGTC	3 cut(s)	649, 1210, 2246
MmeI	TCCRAC	3 cut(s)	417, 683, 1084
Mph1103I	ATGCAT	1 cut(s)	2729
MroI	TCCGGA	2 cut(s)	1749, 2678
MseI	TTAA	4 cut(s)	1539, 1635, 1821, 2607
MspA1I	CMGCKG	1 cut(s)	1421
MspI	CCGG	8 cut(s)	137, 149, 533, 562, 1194, 1311, 1750, 2679
MspR9I	CCNGG	9 cut(s)	137, 226, 259, 306, 562, 801, 1532, 2015, 2580
Mva1269I	GAATGC	2 cut(s)	867, 1996
MvaI	CCWGG	7 cut(s)	226, 259, 306, 801, 1532, 2015, 2580
MwoI	GCNNNNNNNGC	6 cut(s)	38, 131, 154, 163, 230, 2198
NarI	GGCGCC	1 cut(s)	1429
NciI	CCSGG	2 cut(s)	137, 562
NdeI	CATATG	2 cut(s)	381, 451
NheI	GCTAGC	1 cut(s)	1664
NlaIV	GGNNCC	9 cut(s)	141, 153, 399, 441, 1092, 1430, 2301, 2508, 2599
NmeAIII	GCCGAG	1 cut(s)	124
NmuCI	GTSAC	4 cut(s)	1073, 1725, 2428, 2525
NsiI	ATGCAT	1 cut(s)	2729
NspI	RCATGY	3 cut(s)	71, 115, 2704
NspV	TTCGAA	1 cut(s)	161
PagI	TCATGA	1 cut(s)	2008
PceI	AGGCCT	1 cut(s)	293
PciSI	GCTCTTC	1 cut(s)	1239
PctI	GAATGC	2 cut(s)	867, 1996
PfeI	GAWTC	7 cut(s)	254, 526, 1171, 1586, 1642, 1719, 2146
PflMI	CCANNNNNTGG	1 cut(s)	2053
PkrI	GCNGC	7 cut(s)	133, 156, 684, 829, 918, 1420, 1518
PleI	GAGTC	3 cut(s)	649, 1210, 2245
PluTI	GGCGCC	1 cut(s)	1432
PpsI	GAGTC	3 cut(s)	649, 1210, 2245
PpuMI	RGGWCCY	1 cut(s)	2300
PsiI	TTATAA	1 cut(s)	2618
Psp124BI	GAGCTC	1 cut(s)	2203
Psp5II	RGGWCCY	1 cut(s)	2300
Psp6I	CCWGG	7 cut(s)	224, 257, 304, 799, 1530, 2013, 2578
PspEI	GGTNACC	1 cut(s)	1940
PspFI	CCCAGC	2 cut(s)	239, 509
PspGI	CCWGG	7 cut(s)	224, 257, 304, 799, 1530, 2013, 2578
PspN4I	GGNNCC	9 cut(s)	141, 153, 399, 441, 1092, 1430, 2301, 2508, 2599
PspPI	GGNCC	9 cut(s)	222, 439, 549, 901, 1149, 2044, 2300, 2598, 2623
PspPPI	RGGWCCY	1 cut(s)	2300
PstNI	CAGNNNCTG	2 cut(s)	131, 983
PsuI	RGATCY	4 cut(s)	397, 832, 1090, 1316
PvuII	CAGCTG	1 cut(s)	1421
RsaI	GTAC	9 cut(s)	721, 946, 974, 1529, 1981, 2004, 2163, 2271, 2707
RsaNI	GTAC	9 cut(s)	720, 945, 973, 1528, 1980, 2003, 2162, 2270, 2706
SacI	GAGCTC	1 cut(s)	2203
SapI	GCTCTTC	1 cut(s)	1239
SaqAI	TTAA	4 cut(s)	1539, 1635, 1821, 2607
SatI	GCNGC	7 cut(s)	132, 155, 683, 828, 917, 1419, 1517
Sau96I	GGNCC	9 cut(s)	222, 439, 549, 901, 1149, 2044, 2300, 2598, 2623
SchI	GAGTC	3 cut(s)	649, 1210, 2246
ScrFI	CCNGG	9 cut(s)	137, 226, 259, 306, 562, 801, 1532, 2015, 2580
SduI	GDGCHC	3 cut(s)	1234, 2203, 2320
SexAI	ACCWGGT	1 cut(s)	1530
SfaNI	GCATC	4 cut(s)	657, 1231, 1712, 2736
SfoI	GGCGCC	1 cut(s)	1430
SfuI	TTCGAA	1 cut(s)	161
SinI	GGWCC	8 cut(s)	439, 549, 901, 1149, 2044, 2300, 2598, 2623
SmlI	CTYRAG	1 cut(s)	2202
SmoI	CTYRAG	1 cut(s)	2202
SseBI	AGGCCT	1 cut(s)	293
SsiI	CCGC	4 cut(s)	155, 614, 916, 2485
SspDI	GGCGCC	1 cut(s)	1428
SspMI	CTAG	5 cut(s)	24, 566, 1029, 1665, 2766
SstI	GAGCTC	1 cut(s)	2203
StuI	AGGCCT	1 cut(s)	293
StyD4I	CCNGG	9 cut(s)	135, 224, 257, 304, 560, 799, 1530, 2013, 2578
StyI	CCWWGG	1 cut(s)	120
TaaI	ACNGT	5 cut(s)	581, 726, 2083, 2285, 2710
TaiI	ACGT	1 cut(s)	1861
TaqI	TCGA	7 cut(s)	59, 88, 161, 1021, 1477, 1951, 2138
TatI	WGTACW	4 cut(s)	719, 972, 2002, 2269
TauI	GCSGC	2 cut(s)	157, 919
TfiI	GAWTC	7 cut(s)	254, 526, 1171, 1586, 1642, 1719, 2146
Tru1I	TTAA	4 cut(s)	1539, 1635, 1821, 2607
Tru9I	TTAA	4 cut(s)	1539, 1635, 1821, 2607
TscAI	CASTG	7 cut(s)	250, 934, 943, 1255, 1339, 1762, 1932
TseFI	GTSAC	4 cut(s)	1073, 1725, 2428, 2525
TseI	GCWGC	5 cut(s)	131, 682, 827, 1418, 1516
Tsp45I	GTSAC	4 cut(s)	1073, 1725, 2428, 2525
TspRI	CASTG	7 cut(s)	250, 934, 943, 1255, 1339, 1762, 1932
Van91I	CCANNNNNTGG	1 cut(s)	2053
VpaK11BI	GGWCC	8 cut(s)	439, 549, 901, 1149, 2044, 2300, 2598, 2623
XagI	CCTNNNNNAGG	2 cut(s)	870, 2618
XapI	RAATTY	5 cut(s)	277, 932, 1816, 2397, 2545
XceI	RCATGY	3 cut(s)	71, 115, 2704
XmiI	GTMKAC	2 cut(s)	1209, 2748
XspI	CTAG	5 cut(s)	24, 566, 1029, 1665, 2766
Zsp2I	ATGCAT	1 cut(s)	2729

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.