Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001811

Physical Location & Seq

Genomic Coordinates Reverse (-)

33718321 .. 33723941

Gene Structure 5621 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1281300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3060 bp

ATGTCGTGGTTTCGAGCTGGGTCTAGTGTGGCAAAGCTTGCCATTAGGAGAAGTTTGTCGAATAGTGGATCCTATGCGGCTAGAAGGCGGGTCCTTCCGTCCCAAAGCCGAGATTTTCACACCACAATATCTAAATCGAGGGCTGCCCCGGTTCCACGCCCTGTACCCCTCTCCAGGCTAACTGATAGTTTCTTGGATGGAACCAGTAGTGTTTATCTGGAGGGGCTTCAGAGGGCTTGGGAAGCTGATCCCAATAGCGTCGATGAGTCGTGGGACAATTTCTTTAGGAATTTTGTGGGGCAGGCCTCTACCTCTCCCGGAATTTCGGGGCAGACCATTCAAGAGAGTATGCGCTTGTTGTTGCTTGTGAGGGCATACCAGGTTAATGGCCACATGAAGGCTAAATTGGACCCTTTGGGTTTGGAGGAGAGAGATATCCCAGATGATTTGGACCCTGCTCTATATGGGTTCACTGAGTCTGATCTCGACAGGGAGTTCTTTTTGGGGGTCTGGAGGATGGCTGGGTTTTTGTCTGAGAACCGTCCTGTGCAGACCCTTAGGTCCATTTTGACCCGGCTTGAGCAGGCTTACTGTGGGACCATTGGGTATGAGTATATGCACATTGCAGATCGTGAGAGATGTAACTGGTTGAGAGACAAGATTGAGACTCCTACGCCCATGCAATACAATAGGCAGCGCCGTGAGGTTATTCTTGATAGGCTTATTTGGAGTACACAGTTTGAGAACTTCTTGGCTACTAAGTGGACAACAGCAAAGAGGTTCGGACTTGAAGGTGGAGAAACTCTTATTCCGGGCATGAAGGAGATGTTTGATAGGGCTGCTGATCTCGGGGTTGAGAGCATAGTAATTGGAATGCCTCACAGAGGCAGACTGAATGTATTGGGTAATGTGGTGCGGAAGCCTTTGCGTCAGATATTTAGTGAATTTAGTGGTGGTACAAAGCCTGTGGATGAAGTTGGGCTTTACACTGGGACTGGTGATGTCAAGTATCACTTGGGAACCTCTTATGATCGACCCACAAGAGGTGGGAAGAGAATTCATTTATCTTTGGTTGCAAATCCAAGTCACTTGGAAGCTGTGGACCCTGTCGTTGTCGGAAAAACTAGAGCAAAGCAGTATTATTCAAATGATGCGGACAGGACCAAGAATATGGGCATTTTGATTCATGGAGACGGTAGCTTTGCTGGACAAGGTGTGGTCTATGAGACTCTGCATTTGAGTGCTCTTCCTAACTACACCACTGGTGGGACTATACACATTGTGGTGAACAACCAAGTTGCCTTCACAACTGACCCAATGTCGGGAAGATCTTCTCAGTATTGTACTGATGTTGCCAAAGCATTGAATGCCCCGATTTTCCATGTAAATGGTGATGACATGGAGGCAGTCGTTCATGTCTGTGAGCTTGCCGCTGAATGGCGCCAGACTTTCCATTCTGATGTTGTGGTGGATTTAGTGTGCTATCGTCGATTTGGGCATAATGAGATTGACGAGCCATCTTTCACACAGCCCAATATGTACAAGGTTATTCGGAATCATCCCTCAGCTCTAACAATCTACCAGAACAAACTTCTAGAGTCTGGACAAGTGACCAAAGAAGACATTGAAAGGATACAAAACAAGGTTAATTCAATTCTCAATGAAGAGTTCTTGTCTAGCAAGGATTATGTTCCTCAAAGAAGGGACTGGCTTTCATCTCATTGGTCTGGATTTAAGTCACCTGAGCAGATTTCTCGTATCCGGAATACTGGGGTAAAACCAGAGATTTTGAAGAGTGTTGGCAAAGCTGTCACTGCCCTTCCAGAAACTTTTAAACCTCACAGAGCAGTAAAGAAGGTGTATGAACAACGTGGACAAATGATTGAGACTGGTGAAGGGATTGATTGGGCAGTTGCTGAAGCACTTGCCTTTGCCACACTACTAGTAGAAGGTAACCATGTTCGACTGAGTGGTCAGGATGTTGAAAGAGGTACCTTTAGTCATCGGCATTCTGTTCTTCACGACCAGGAAACTGGGGAAAAGTATTGTCCTCTTGACCATATTACGGCTGGCCAAGATGAGGAAATGTTTACAGTTAGCAACAGCTCACTCTCAGAGTTTGGTGTTCTTGGATTTGAATTGGGTTACTCGATGGAGAGTCCAAATGCATTAGTGATTTGGGAAGCTCAATTTGGTGATTTTGCCAATGGAGCTCATGTTATCTTTGATCAGTTTTTGAGTAGTGGGGAGTCCAAGTGGCTTCGTCAAACTGGGCTTGTTGTGCTGCTTCCTCATGGTTACGATGGCCAGGGCCCTGAACATTCTAGTGCCCGCCTAGAGCGATTTCTTCAGATGAGTGATGACAATCCTTTTGTCATACCTGAGATGGATCCCACACTTCGAAAGCAAATTCAGGAATGCAATTGGCAGGTTGTGAATGTGACAACTCCTGCCAACTACTTCCATGTACTGCGTCGTCAGATACACAGGGAATTCCGCAAGCCTCTTATTGTTATGGCTCCTAAAAACCTTCTACGGCACAAGGACTGCAAATCAAATTTATCTGAGTTTGATGATGTGCAAGGCCACCCAGGTTTTGACAAGCAGGGGACTAGATTTAAGCGACTCATCAAGGACCAGAATCACCACTCTGATCTTGAGGAGGGTATTAGACGGCTGGTTCTGTGCTCTGGCAAGGTTTATTATGAGCTTGATGAACAGCGAAGAAAGGTTGAAGCAAAGGATGTTGCAATTTGTAGAGTGGAACAGCTTTGCCCCTTCCCGTATGACCTTATCCAGCGAGAGCTGAAGCGATATCCAAATGCTGAGATAGTTTGGGTCCAGGAAGAGCCAATGAACATGGGTGCCTACAGCTATATTGCACCCCGCCTTAGCACTGCCATGAAGTCCCTGGGTAGAGGAACTATCGACGACATCAAATATATTGGCCGTGCTCCATCAGCTGCATCAGCCACTGGTTTCTATTCGGTTCATTTGAAGGAACAAAGTGAGATTGTACAGAAAGCGGTACAGAAGGAGCCAATTGAATCTCACAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004591 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005947 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0008150 GO:0008152 GO:0009060 GO:0009353 GO:0009987 GO:0015980 GO:0016491 GO:0016624 GO:0016903 GO:0016999 GO:0017144 GO:0019752 GO:0030062 GO:0031974 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045239 GO:0045240 GO:0045252 GO:0045333 GO:0055114 GO:0070013 GO:0071704 GO:0072350 GO:0098798 GO:1902494 GO:1990204 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00020 ko00310 ko00380 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map00380 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

2-oxogl_dehyd_N E1_dh OxoGdeHyase_C Transket_pyr

Protein Analysis

1020

Amino Acids

115.31

Weight (kDa)

6.58

Isoelectric Point (pI)

41.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-oxogl_dehyd_N	PF16078	62 - 99	3.7e-14	2-oxoglutarate dehydrogenase N-terminus
E1_dh	PF00676	243 - 563	4.5e-59	Dehydrogenase E1 component
Transket_pyr	PF02779	633 - 846	9.6e-50	Transketolase, pyrimidine binding domain
OxoGdeHyase_C	PF16870	869 - 1009	5.3e-50	2-oxoglutarate dehydrogenase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001811)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55410 AT3G55410 AT5G65750
fragaria_vesca	FvH4_7g21770 FvH4_7g21770 FvH4_7g21770 FvH4_7g21770 FvH4_7g21770 FvH4_7g21770 FvH4_7g21851
malus_domestica	MD01G1122800.v1.1 MD02G1281300.v1.1 MD02G1281500.v1.1 MD07G1192300.v1.1
prunus_persica	Prupe.2G230300_v2.0.a1 Prupe.2G230400_v2.0.a1 Prupe.2G230400_v2.0.a1 Prupe.2G230400_v2.0.a1
pyrus_communis	pycom01g14980 pycom02g24050 pycom07g18180
rosa_chinensis	RchiOBHm_Chr1g0365921 RchiOBHm_Chr1g0366031
rosa_laevigata	RLG00000027377 RLG00000027378
rosa_multiflora	Rmu_co8265029.1_g000001 Rmu_sc0004288.1_g000005 Rmu_sc0006406.1_g000008
rosa_roxburghii	Rroxscaffold_4G00290620 Rroxscaffold_4G00290640
rosa_rugosa	Rorug01G0325800 Rorug01G0325900 Rorug01G0326000 Rorug01G0326100 Rorug01G0326200 Rorug01G0326300 Rorug01G0326400
rosa_samantha	Rh1AG334500 Rh1AG334600 Rh1BG295400 Rh1BG295600 Rh1CG312100 Rh1CG312200 Rh1DG327700 Rh1DG328000
rosa_wichuraiana	Rw1G029690 Rw1G029720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	559, 1971
Acc36I	ACCTGC	1 cut(s)	2419
Acc65I	GGTACC	1 cut(s)	1991
AccB1I	GGYRCC	3 cut(s)	1440, 1991, 2864
AccIII	TCCGGA	1 cut(s)	1761
AciI	CCGC	9 cut(s)	77, 88, 916, 1154, 1431, 2332, 2497, 2887, 3026
AclWI	GGATC	5 cut(s)	63, 76, 242, 2384, 2397
AcoI	YGGCCR	4 cut(s)	388, 2071, 2305, 2947
AcsI	RAATTY	7 cut(s)	289, 321, 944, 1056, 2409, 2492, 2557
AcuI	CTGAAG	4 cut(s)	212, 1938, 2333, 2828
AcyI	GRCGYC	1 cut(s)	1441
AdeI	CACNNNGTG	1 cut(s)	1282
AfaI	GTAC	9 cut(s)	165, 733, 958, 1345, 1541, 1993, 2469, 3018, 3030
AhdI	GACNNNNNGTC	1 cut(s)	1318
AhlI	ACTAGT	1 cut(s)	1942
AjnI	CCWGG	7 cut(s)	173, 378, 2025, 2307, 2590, 2841, 2910
AjuI	GAANNNNNNNTTGG	4 cut(s)	389, 421, 2175, 2207
Alw21I	GWGCWC	4 cut(s)	1246, 2215, 2690, 2956
Alw26I	GTCTC	5 cut(s)	648, 659, 1185, 1220, 1880
AlwI	GGATC	5 cut(s)	63, 76, 242, 2384, 2397
AlwNI	CAGNNNCTG	2 cut(s)	1916, 2975
Ama87I	CYCGRG	1 cut(s)	848
Aor13HI	TCCGGA	1 cut(s)	1761
AoxI	GGCC	7 cut(s)	303, 388, 2071, 2305, 2311, 2584, 2947
ApaI	GGGCCC	1 cut(s)	2315
ApeKI	GCWGC	5 cut(s)	143, 694, 839, 2284, 2963
ApoI	RAATTY	7 cut(s)	289, 321, 944, 1056, 2409, 2492, 2557
ArsI	GACNNNNNNTTYG	6 cut(s)	1184, 1216, 1219, 1251, 2354, 2386
Asp700I	GAANNNNTTC	1 cut(s)	1330
Asp718I	GGTACC	1 cut(s)	1991
AspLEI	GCGC	3 cut(s)	354, 699, 1443
AsuC2I	CCSGG	4 cut(s)	149, 318, 574, 813
AsuHPI	GGTGA	7 cut(s)	1010, 1297, 1403, 1731, 1904, 2207, 2636
AsuII	TTCGAA	1 cut(s)	2401
AvaI	CYCGRG	1 cut(s)	848
AvaII	GGWCC	9 cut(s)	91, 409, 451, 561, 597, 1102, 1161, 2635, 2839
AxyI	CCTNAGG	1 cut(s)	557
BaeGI	GKGCMC	2 cut(s)	2315, 2332
BalI	TGGCCA	3 cut(s)	390, 2073, 2307
BamHI	GGATCC	2 cut(s)	68, 2389
BanI	GGYRCC	3 cut(s)	1440, 1991, 2864
BanII	GRGCYC	2 cut(s)	2215, 2315
BbsI	GAAGAC	1 cut(s)	1626
Bbv12I	GWGCWC	4 cut(s)	1246, 2215, 2690, 2956
BbvCI	CCTCAGC	1 cut(s)	1564
BbvI	GCAGC	5 cut(s)	130, 706, 826, 2271, 2950
BccI	CCATC	7 cut(s)	191, 511, 1525, 2146, 2297, 2380, 2965
BceAI	ACGGC	5 cut(s)	684, 2082, 2552, 2690, 2934
BcgI	CGANNNNNNTGC	2 cut(s)	1736, 1770
BciT130I	CCWGG	7 cut(s)	175, 380, 2027, 2309, 2592, 2843, 2912
BciVI	GTATCC	2 cut(s)	1626, 1769
BclI	TGATCA	1 cut(s)	2227
BcnI	CCSGG	4 cut(s)	149, 318, 574, 813
BcoDI	GTCTC	5 cut(s)	648, 659, 1185, 1220, 1880
BcuI	ACTAGT	1 cut(s)	1942
BfaI	CTAG	9 cut(s)	24, 81, 1125, 1595, 1677, 1943, 2325, 2336, 2613
BfmI	CTRYAG	1 cut(s)	2869
BfoI	RGCGCY	2 cut(s)	700, 1444
BfuAI	ACCTGC	1 cut(s)	2419
BfuI	GTATCC	2 cut(s)	1626, 1769
BglII	AGATCT	1 cut(s)	1328
BisI	GCNGC	7 cut(s)	78, 144, 695, 840, 1431, 2285, 2964
BlsI	GCNGC	7 cut(s)	79, 145, 696, 841, 1432, 2286, 2965
Bme18I	GGWCC	9 cut(s)	91, 409, 451, 561, 597, 1102, 1161, 2635, 2839
BmeRI	GACNNNNNGTC	1 cut(s)	1318
BmeT110I	CYCGRG	1 cut(s)	848
BmrI	ACTGGG	4 cut(s)	999, 1779, 2043, 2280
BmsI	GCATC	2 cut(s)	1141, 2975
BmuI	ACTGGG	4 cut(s)	999, 1779, 2043, 2280
BpiI	GAAGAC	1 cut(s)	1626
BpmI	CTGGAG	3 cut(s)	157, 239, 532
Bpu10I	CCTNAGC	3 cut(s)	1564, 1743, 2891
Bpu14I	TTCGAA	1 cut(s)	2401
BpuEI	CTTGAG	2 cut(s)	599, 2678
BpuMI	CCSGG	4 cut(s)	149, 318, 574, 813
BsaBI	GATNNNNATC	1 cut(s)	2364
BsaHI	GRCGYC	1 cut(s)	1441
BsaJI	CCNNGG	5 cut(s)	147, 2308, 2590, 2910, 2911
BsaWI	WCCGGW	1 cut(s)	1761
BsaXI	ACNNNNNCTCC	4 cut(s)	2202, 2232, 2240, 2270
Bse21I	CCTNAGG	1 cut(s)	557
Bse3DI	GCAATG	1 cut(s)	621
Bse8I	GATNNNNATC	1 cut(s)	2364
BseAI	TCCGGA	1 cut(s)	1761
BseBI	CCWGG	7 cut(s)	175, 380, 2027, 2309, 2592, 2843, 2912
BseDI	CCNNGG	5 cut(s)	147, 2308, 2590, 2910, 2911
BseGI	GGATG	6 cut(s)	202, 522, 976, 1558, 1984, 2749
BseJI	GATNNNNATC	1 cut(s)	2364
BseMI	GCAATG	1 cut(s)	621
BseRI	GAGGAG	2 cut(s)	440, 2675
BseSI	GKGCMC	2 cut(s)	2315, 2332
BseXI	GCAGC	5 cut(s)	130, 706, 826, 2271, 2950
BseYI	CCCAGC	2 cut(s)	17, 521
BsgI	GTGCAG	1 cut(s)	569
BshFI	GGCC	7 cut(s)	305, 390, 2073, 2307, 2313, 2586, 2949
BshNI	GGYRCC	3 cut(s)	1440, 1991, 2864
BsiHKAI	GWGCWC	4 cut(s)	1246, 2215, 2690, 2956
BsiHKCI	CYCGRG	1 cut(s)	848
BsiSI	CCGG	5 cut(s)	149, 318, 574, 812, 1762
BslFI	GGGAC	8 cut(s)	85, 287, 610, 1006, 1282, 1718, 2623, 2893
BsmAI	GTCTC	5 cut(s)	648, 659, 1185, 1220, 1880
BsmBI	CGTCTC	1 cut(s)	1185
BsmFI	GGGAC	8 cut(s)	85, 287, 610, 1006, 1282, 1718, 2623, 2893
BsmI	GAATGC	4 cut(s)	879, 1372, 2008, 2423
BsnI	GGCC	7 cut(s)	305, 390, 2073, 2307, 2313, 2586, 2949
BsoBI	CYCGRG	1 cut(s)	848
Bsp119I	TTCGAA	1 cut(s)	2401
Bsp120I	GGGCCC	1 cut(s)	2311
Bsp1286I	GDGCHC	6 cut(s)	1246, 2215, 2315, 2332, 2690, 2956
Bsp13I	TCCGGA	1 cut(s)	1761
Bsp1407I	TGTACA	2 cut(s)	1539, 3016
BspACI	CCGC	9 cut(s)	77, 88, 916, 1154, 1431, 2332, 2497, 2887, 3026
BspANI	GGCC	7 cut(s)	305, 390, 2073, 2307, 2313, 2586, 2949
BspEI	TCCGGA	1 cut(s)	1761
BspMI	ACCTGC	1 cut(s)	2419
BspPI	GGATC	5 cut(s)	63, 76, 242, 2384, 2397
BspQI	GCTCTTC	2 cut(s)	1251, 2841
BspT104I	TTCGAA	1 cut(s)	2401
BspT107I	GGYRCC	3 cut(s)	1440, 1991, 2864
BsrDI	GCAATG	1 cut(s)	621
BsrGI	TGTACA	2 cut(s)	1539, 3016
BssECI	CCNNGG	5 cut(s)	147, 2308, 2590, 2910, 2911
BssNI	GRCGYC	1 cut(s)	1441
Bst2UI	CCWGG	7 cut(s)	175, 380, 2027, 2309, 2592, 2843, 2912
Bst4CI	ACNGT	5 cut(s)	542, 593, 738, 1196, 2095
Bst6I	CTCTTC	5 cut(s)	1046, 1251, 1659, 1787, 2841
BstACI	GRCGYC	1 cut(s)	1441
BstAPI	GCANNNNNTGC	2 cut(s)	38, 1367
BstAUI	TGTACA	2 cut(s)	1539, 3016
BstBI	TTCGAA	1 cut(s)	2401
BstC8I	GCNNGC	7 cut(s)	39, 303, 585, 1428, 2071, 2332, 2501
BstEII	GGTNACC	1 cut(s)	1952
BstENI	CCTNNNNNAGG	1 cut(s)	882
BstF5I	GGATG	6 cut(s)	202, 522, 976, 1558, 1984, 2749
BstH2I	RGCGCY	2 cut(s)	700, 1444
BstHHI	GCGC	3 cut(s)	354, 699, 1443
BstMAI	GTCTC	5 cut(s)	648, 659, 1185, 1220, 1880
BstMWI	GCNNNNNNNGC	8 cut(s)	38, 242, 1367, 1814, 2210, 2281, 2960, 2969
BstNI	CCWGG	7 cut(s)	175, 380, 2027, 2309, 2592, 2843, 2912
BstPI	GGTNACC	1 cut(s)	1952
BstSFI	CTRYAG	1 cut(s)	2869
BstSLI	GKGCMC	2 cut(s)	2315, 2332
BstV1I	GCAGC	5 cut(s)	130, 706, 826, 2271, 2950
BstV2I	GAAGAC	1 cut(s)	1626
BstX2I	RGATCY	3 cut(s)	68, 1328, 2389
BstXI	CCANNNNNNTGG	4 cut(s)	386, 1171, 1388, 2033
BstYI	RGATCY	3 cut(s)	68, 1328, 2389
Bsu36I	CCTNAGG	1 cut(s)	557
BsuI	GTATCC	2 cut(s)	1626, 1769
BsuRI	GGCC	7 cut(s)	305, 390, 2073, 2307, 2313, 2586, 2949
BtsCI	GGATG	6 cut(s)	202, 522, 976, 1558, 1984, 2749
BtsI	GCAGTG	2 cut(s)	1812, 2895
BtsIMutI	CAGTG	6 cut(s)	471, 987, 1260, 1812, 2895, 2973
BveI	ACCTGC	1 cut(s)	2419
Cac8I	GCNNGC	7 cut(s)	39, 303, 585, 1428, 2071, 2332, 2501
CaiI	CAGNNNCTG	2 cut(s)	1916, 2975
CfoI	GCGC	3 cut(s)	354, 699, 1443
CseI	GACGC	3 cut(s)	247, 917, 2462
CsiI	ACCWGGT	1 cut(s)	378
Csp6I	GTAC	9 cut(s)	164, 732, 957, 1344, 1540, 1992, 2468, 3017, 3029
CspCI	CAANNNNNGTGG	2 cut(s)	948, 983
CviQI	GTAC	9 cut(s)	164, 732, 957, 1344, 1540, 1992, 2468, 3017, 3029
DinI	GGCGCC	1 cut(s)	1442
DraI	TTTAAA	1 cut(s)	1834
DraIII	CACNNNGTG	1 cut(s)	1282
DrdI	GACNNNNNNGTC	2 cut(s)	559, 1971
DriI	GACNNNNNGTC	1 cut(s)	1318
DseDI	GACNNNNNNGTC	2 cut(s)	559, 1971
EaeI	YGGCCR	4 cut(s)	388, 2071, 2305, 2947
Eam1104I	CTCTTC	5 cut(s)	1046, 1251, 1659, 1787, 2841
Eam1105I	GACNNNNNGTC	1 cut(s)	1318
EarI	CTCTTC	5 cut(s)	1046, 1251, 1659, 1787, 2841
Ecl136II	GAGCTC	1 cut(s)	2213
Eco147I	AGGCCT	1 cut(s)	305
Eco24I	GRGCYC	2 cut(s)	2215, 2315
Eco32I	GATATC	2 cut(s)	436, 2816
Eco47I	GGWCC	9 cut(s)	91, 409, 451, 561, 597, 1102, 1161, 2635, 2839
Eco53kI	GAGCTC	1 cut(s)	2213
Eco57I	CTGAAG	4 cut(s)	212, 1938, 2333, 2828
Eco81I	CCTNAGG	1 cut(s)	557
Eco88I	CYCGRG	1 cut(s)	848
Eco91I	GGTNACC	1 cut(s)	1952
EcoICRI	GAGCTC	1 cut(s)	2213
EcoNI	CCTNNNNNAGG	1 cut(s)	882
EcoO109I	RGGNCCY	3 cut(s)	91, 2311, 2312
EcoO65I	GGTNACC	1 cut(s)	1952
EcoRI	GAATTC	2 cut(s)	1056, 2492
EcoRII	CCWGG	7 cut(s)	173, 378, 2025, 2307, 2590, 2841, 2910
EcoRV	GATATC	2 cut(s)	436, 2816
EcoT22I	ATGCAT	1 cut(s)	2170
EcoT38I	GRGCYC	2 cut(s)	2215, 2315
EgeI	GGCGCC	1 cut(s)	1442
EheI	GGCGCC	1 cut(s)	1442
Esp3I	CGTCTC	1 cut(s)	1185
FalI	AAGNNNNNCTT	4 cut(s)	966, 998, 998, 1030
FaqI	GGGAC	8 cut(s)	85, 287, 610, 1006, 1282, 1718, 2623, 2893
FauI	CCCGC	3 cut(s)	81, 2339, 2894
FbaI	TGATCA	1 cut(s)	2227
Fnu4HI	GCNGC	7 cut(s)	78, 144, 695, 840, 1431, 2285, 2964
FokI	GGATG	6 cut(s)	209, 529, 983, 1545, 1991, 2756
FriOI	GRGCYC	2 cut(s)	2215, 2315
Fsp4HI	GCNGC	7 cut(s)	78, 144, 695, 840, 1431, 2285, 2964
FspBI	CTAG	9 cut(s)	24, 81, 1125, 1595, 1677, 1943, 2325, 2336, 2613
GlaI	GCGC	3 cut(s)	353, 698, 1442
GluI	GCNGC	7 cut(s)	78, 144, 695, 840, 1431, 2285, 2964
GsaI	CCCAGC	2 cut(s)	21, 525
GsuI	CTGGAG	3 cut(s)	157, 239, 532
HaeII	RGCGCY	2 cut(s)	700, 1444
HaeIII	GGCC	7 cut(s)	305, 390, 2073, 2307, 2313, 2586, 2949
HapII	CCGG	5 cut(s)	149, 318, 574, 812, 1762
HgaI	GACGC	3 cut(s)	247, 917, 2462
HhaI	GCGC	3 cut(s)	354, 699, 1443
Hin1I	GRCGYC	1 cut(s)	1441
Hin6I	GCGC	3 cut(s)	352, 697, 1441
HinP1I	GCGC	3 cut(s)	352, 697, 1441
HindIII	AAGCTT	1 cut(s)	35
HpaII	CCGG	5 cut(s)	149, 318, 574, 812, 1762
HphI	GGTGA	7 cut(s)	1010, 1297, 1403, 1731, 1904, 2207, 2636
Hpy166II	GTNNAC	7 cut(s)	471, 734, 765, 1102, 1288, 1874, 2091
Hpy8I	GTNNAC	7 cut(s)	471, 734, 765, 1102, 1288, 1874, 2091
Hpy99I	CGWCG	4 cut(s)	263, 1491, 2478, 2933
HpyCH4III	ACNGT	5 cut(s)	542, 593, 738, 1196, 2095
HpyCH4IV	ACGT	1 cut(s)	1870
HpyF10VI	GCNNNNNNNGC	8 cut(s)	38, 242, 1367, 1814, 2210, 2281, 2960, 2969
HpySE526I	ACGT	1 cut(s)	1870
Hsp92I	GRCGYC	1 cut(s)	1441
HspAI	GCGC	3 cut(s)	352, 697, 1441
KasI	GGCGCC	1 cut(s)	1440
Kpn2I	TCCGGA	1 cut(s)	1761
KpnI	GGTACC	1 cut(s)	1995
Ksp22I	TGATCA	1 cut(s)	2227
LguI	GCTCTTC	2 cut(s)	1251, 2841
LmnI	GCTCC	4 cut(s)	2210, 2524, 2959, 3037
Lsp1109I	GCAGC	5 cut(s)	130, 706, 826, 2271, 2950
LweI	GCATC	2 cut(s)	1141, 2975
MabI	ACCWGGT	1 cut(s)	378
MaeI	CTAG	9 cut(s)	24, 81, 1125, 1595, 1677, 1943, 2325, 2336, 2613
MaeII	ACGT	1 cut(s)	1870
MaeIII	GTNAC	9 cut(s)	641, 1085, 1609, 1737, 1810, 1952, 2144, 2297, 2440
MfeI	CAATTG	2 cut(s)	2422, 3042
MflI	RGATCY	3 cut(s)	68, 1328, 2389
MhlI	GDGCHC	6 cut(s)	1246, 2215, 2315, 2332, 2690, 2956
MlsI	TGGCCA	3 cut(s)	390, 2073, 2307
MluNI	TGGCCA	3 cut(s)	390, 2073, 2307
Mly113I	GGCGCC	1 cut(s)	1441
MlyI	GAGTC	8 cut(s)	275, 485, 661, 1222, 1607, 2167, 2258, 2619
MmeI	TCCRAC	1 cut(s)	1096
Mox20I	TGGCCA	3 cut(s)	390, 2073, 2307
Mph1103I	ATGCAT	1 cut(s)	2170
MroI	TCCGGA	1 cut(s)	1761
MroXI	GAANNNNTTC	1 cut(s)	1330
MscI	TGGCCA	3 cut(s)	390, 2073, 2307
MseI	TTAA	5 cut(s)	384, 1647, 1734, 1833, 2619
MslI	CAYNNNNRTG	7 cut(s)	1239, 1283, 1386, 1419, 1458, 2325, 2900
Msp20I	TGGCCA	3 cut(s)	390, 2073, 2307
MspA1I	CMGCKG	3 cut(s)	1433, 2963, 3057
MspI	CCGG	5 cut(s)	149, 318, 574, 812, 1762
MunI	CAATTG	2 cut(s)	2422, 3042
Mva1269I	GAATGC	4 cut(s)	879, 1372, 2008, 2423
MvaI	CCWGG	7 cut(s)	175, 380, 2027, 2309, 2592, 2843, 2912
MwoI	GCNNNNNNNGC	8 cut(s)	38, 242, 1367, 1814, 2210, 2281, 2960, 2969
NarI	GGCGCC	1 cut(s)	1441
NciI	CCSGG	4 cut(s)	149, 318, 574, 813
NmeAIII	GCCGAG	1 cut(s)	134
NmuCI	GTSAC	5 cut(s)	1085, 1609, 1737, 1810, 2440
NsiI	ATGCAT	1 cut(s)	2170
NspV	TTCGAA	1 cut(s)	2401
PasI	CCCWGGG	1 cut(s)	2911
PceI	AGGCCT	1 cut(s)	305
PciSI	GCTCTTC	2 cut(s)	1251, 2841
PctI	GAATGC	4 cut(s)	879, 1372, 2008, 2423
PdmI	GAANNNNTTC	1 cut(s)	1330
PfeI	GAWTC	4 cut(s)	1183, 1555, 2641, 3047
PflFI	GACNNNGTC	1 cut(s)	1106
PfoI	TCCNGGA	2 cut(s)	316, 2841
PkrI	GCNGC	7 cut(s)	79, 145, 696, 841, 1432, 2286, 2965
PleI	GAGTC	8 cut(s)	274, 484, 661, 1222, 1606, 2166, 2257, 2619
PluTI	GGCGCC	1 cut(s)	1444
PpsI	GAGTC	8 cut(s)	274, 484, 661, 1222, 1606, 2166, 2257, 2619
PpuMI	RGGWCCY	1 cut(s)	91
Psp124BI	GAGCTC	1 cut(s)	2215
Psp5II	RGGWCCY	1 cut(s)	91
Psp6I	CCWGG	7 cut(s)	173, 378, 2025, 2307, 2590, 2841, 2910
PspEI	GGTNACC	1 cut(s)	1952
PspFI	CCCAGC	2 cut(s)	17, 521
PspGI	CCWGG	7 cut(s)	173, 378, 2025, 2307, 2590, 2841, 2910
PspOMI	GGGCCC	1 cut(s)	2311
PspPPI	RGGWCCY	1 cut(s)	91
PstNI	CAGNNNCTG	2 cut(s)	1916, 2975
PsuI	RGATCY	3 cut(s)	68, 1328, 2389
PsyI	GACNNNGTC	1 cut(s)	1106
PvuII	CAGCTG	2 cut(s)	2963, 3057
RsaI	GTAC	9 cut(s)	165, 733, 958, 1345, 1541, 1993, 2469, 3018, 3030
RsaNI	GTAC	9 cut(s)	164, 732, 957, 1344, 1540, 1992, 2468, 3017, 3029
RseI	CAYNNNNRTG	7 cut(s)	1239, 1283, 1386, 1419, 1458, 2325, 2900
SacI	GAGCTC	1 cut(s)	2215
SapI	GCTCTTC	2 cut(s)	1251, 2841
SaqAI	TTAA	5 cut(s)	384, 1647, 1734, 1833, 2619
SatI	GCNGC	7 cut(s)	78, 144, 695, 840, 1431, 2285, 2964
SchI	GAGTC	8 cut(s)	275, 485, 661, 1222, 1607, 2167, 2258, 2619
SduI	GDGCHC	6 cut(s)	1246, 2215, 2315, 2332, 2690, 2956
SexAI	ACCWGGT	1 cut(s)	378
SfaNI	GCATC	2 cut(s)	1141, 2975
SfcI	CTRYAG	1 cut(s)	2869
SfoI	GGCGCC	1 cut(s)	1442
SfuI	TTCGAA	1 cut(s)	2401
SinI	GGWCC	9 cut(s)	91, 409, 451, 561, 597, 1102, 1161, 2635, 2839
SmiMI	CAYNNNNRTG	7 cut(s)	1239, 1283, 1386, 1419, 1458, 2325, 2900
SmlI	CTYRAG	2 cut(s)	578, 2657
SmoI	CTYRAG	2 cut(s)	578, 2657
SpeI	ACTAGT	1 cut(s)	1942
SseBI	AGGCCT	1 cut(s)	305
SsiI	CCGC	9 cut(s)	77, 88, 916, 1154, 1431, 2332, 2497, 2887, 3026
SspDI	GGCGCC	1 cut(s)	1440
SspMI	CTAG	9 cut(s)	24, 81, 1125, 1595, 1677, 1943, 2325, 2336, 2613
SstI	GAGCTC	1 cut(s)	2215
StuI	AGGCCT	1 cut(s)	305
TaaI	ACNGT	5 cut(s)	542, 593, 738, 1196, 2095
TaiI	ACGT	1 cut(s)	1873
TatI	WGTACW	5 cut(s)	731, 1343, 1539, 2467, 3016
TauI	GCSGC	2 cut(s)	80, 1433
TfiI	GAWTC	4 cut(s)	1183, 1555, 2641, 3047
Tru1I	TTAA	5 cut(s)	384, 1647, 1734, 1833, 2619
Tru9I	TTAA	5 cut(s)	384, 1647, 1734, 1833, 2619
TscAI	CASTG	6 cut(s)	478, 994, 1267, 1819, 2902, 2980
TseFI	GTSAC	5 cut(s)	1085, 1609, 1737, 1810, 2440
TseI	GCWGC	5 cut(s)	143, 694, 839, 2284, 2963
Tsp45I	GTSAC	5 cut(s)	1085, 1609, 1737, 1810, 2440
TspGWI	ACGGA	1 cut(s)	87
TspRI	CASTG	6 cut(s)	478, 994, 1267, 1819, 2902, 2980
Tth111I	GACNNNGTC	1 cut(s)	1106
VpaK11BI	GGWCC	9 cut(s)	91, 409, 451, 561, 597, 1102, 1161, 2635, 2839
XagI	CCTNNNNNAGG	1 cut(s)	882
XapI	RAATTY	7 cut(s)	289, 321, 944, 1056, 2409, 2492, 2557
XbaI	TCTAGA	1 cut(s)	1594
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2066, 2908
XmnI	GAANNNNTTC	1 cut(s)	1330
XspI	CTAG	9 cut(s)	24, 81, 1125, 1595, 1677, 1943, 2325, 2336, 2613
Zsp2I	ATGCAT	1 cut(s)	2170

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.