Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0001811

Physical Location & Seq

Genomic Coordinates Forward (+)

27197418 .. 27203333

Gene Structure 5916 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1192300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3036 bp

ATGGCGTGGTTTAGAGCAGTGTCTAGTGTGGCAAAGCTTGCCATTAGGAGAAATTTATCAAATTGTGGATCATATATGGCTAGGAGTCGGGTTCTTCCGTCTCAAAATCGGGACTTTCACACCACATTATGTAAGGCTCAATCTGCCCCGATTCCACGCCCTGTACCCCTCTCTAGACTAAATGATAGTTTCTTGGATGGAACCAGTAGTGTTTATTTGGAGGGGCTTCAGAGGGATTGGGAAGCCGACCCAAATAGTGTTGATGAGTCCTGGGACAATTTCTTTAGGAATTTTGTAGGTCAGGCCTCTACCTCGCCCGGAGTTTCAGGCCAAACCATTCAAGAGAGTATGCGCTTGTTGTTGCTTGTGAGGGCATACCAGGTTAGTGGTCACATGAAAGCTCAGTTGGACCCTTTGGGTTTGGAAGAGAGAGATATCCCAGATGATTTGGACCCTGCTCTATATGGGTTCACAGAGGCTGATCTTGATAGGGAGTTCTTTTTAGGGGTCTGGAGGATGTCTGGGTTTTTGGCTGAGAACCGTCCCGTGCAGACCCTTAGGTCCATATTGACCCGGCTTGAGCAGGCTTATTGTGGGACCATTGGGTACGAGTATATGCACATTGCCGATCGTAATCGATGTAACTGGTTGAGAGACAAGATTGAGACTCCAACGCCAATGCAGTACAATAGGCAACGTCGTGAGGTTATTCTTGATAGACTTATGTGGAGTACACAGTTTGAGAACTTCTTGGCCACTAAGTGGAAAGCAGCTAAGAGGTTCGGCCTTGAAGGTGGAGAAACTCTTATTCCCGGCATGAAGGAGATGTTTGACAGGGCTGCTGATCTCGGGGTTGAGGGCATAGTTATTGGAATGCCTCACAGAGGGAGACTAAATGTATTGGGTAATGTGGTGCGGAAGCCTTTGCGTCAGATATTTAATGAATTCAGTGGTGGTACAAAGCCTGTGGATGAAGATGGGCTATACACTGGGACTGGTGATGTCAAGTATCACTTGGGAACCTCTTATGATAGACCCACTAGAGGTGGCAAGAGACTTCATTTATCTTTGGTTGCAAATCCAAGTCACTTGGAAGCTGTGGACCCTGTGGTTGTCGGAAAAACTAGAGCAAAGCAGTTTTATTCAAATGACGTGGACAGGACCAAGAATATGGGTGTCTTGATTCATGGAGATGGCAGCTTTGCTGGACAAGGTGTGGTGTACGAGACTCTGCATCTAAGCGCTCTTCCTAACTACACCACTGGTGGGACTATACACATTGTGGTGAATAACCAAGTTGCCTTCACAACTGACCCAATGTCTGGAAGATCTTCAGAGTATTGTACAGATGTCGCCAAAGCATTGAATGCTCCTATTTTCCATGTAAATGCTGATGACATGGAGGCAGTCGCTCATGTCTGCGAGCTTGCTGCTGAATGGCGCCAGACATTCCATTCTGATGTTGTGGTTGATTTAGTGTGCTATCGTCGATTTGGGCATAATGAGATGGATGAACCATCTTTCACGCAGCCGGAAATGTACAAGGTTATTCGAAATCATCCCCCAGCTCTGACAATCTACCAGAACAAACTTCTAGAATCTGCACAAGTGACCAAAGAAGACATTGAAAGGATACAAAACAAGGTTAATTCAATTCTCAATGAAGAATTGTTGGCTAGCAAGGATTATGTTCCTCAAAAAAGAGACTGGCTTGCATCCCATTGGTCTGGATTTAAGTCACCTGAACAAATTTCTCGTATCCAGAATACTGGGGTAAAACCAGAGATTTTGAAGAGTGTTGGCAAATCAGTCACAGCCCTTCCAGAAACTTTTAAGGCTCACAAAGGAGTAAAGAAGGTCTATGAACAACGCGCACAAATGATTGAGACTGGTGAAGGAATTGATTGGGCAGTTGCAGAAGCACTTGCCTTTGCTACATTAATTGTAGAAGGTAACCATGTTCGACTGAGTGGTCAGGATGTTGAAAGAGGTACATTTAGTCATCGTCATTCAGTAGTTCACGACCAGGAAACTGGAGAAAAGTATTGCCCTCTTGACAATATTATGGCGGACCAAGATGAGGAACTGTTTACAGTTAGCAACAGTTCACTCTCGGAGTTTGGTGTTCTTGGATTTGAATTGGGTTACTCGATGGAAAGTCCAAACTCATTAGTAATCTGGGAAGCTCAGTTTGGTGATTTTTCCAATGGAGCTCAAGTTATATTTGATCAGTTTTTGAGTGGTGGGGAGTCCAAGTGGCTTCGTCAAACCGGGCTTGTTGTGTTGCTTCCTCATGGTTACATGGGCCAGGGCCCTGAACATTCTAGTGCACGACTGGAACGATTTCTTCAGATGAGTGATGACAATCCCTTTGTCATACCTGAGATGGATTCCACACATCGGAAGCAAATTCAGGAATGCAATTGGCAGGTTGTGAATTTGACAACTCCTGCAAACTACTTCCATGTTCTGCGTCGTCAGATACACAGGGAATTCCGCAAGCCTCTTATTGTGATGGCTCCTAAAAACCTTCTACGGCACAAGGACTGCAAATCAAATTTATCCGAGTTCGATGATGTGCAAGGCCACCCAGGTTTTGACAAGCAGGGGACCAGATTTAAGCGCCTCATCAAGGACCAGAATCACCACTCTGATCTTGAGGAGGGTATTAGACGGCTGGTTCTATGCTCCGGCAAGCTTTATTATGAACTTGATGGAGAGCGAATAAAGGTTGGAGCAAAGGATGTTGCGATTTGTAGAGTGGAACAGCTTTGCCCCTTCCCTTACGACCTCATCCAGCGAGAGCTGAAACGATATCCAAATGCTGAGATAGTTTGGTGTCAGGAAGAGCCGATGAACATGGGTGCGTACAGCTACATTGCACCCCGCCTTAGTGCCGCCATGAAGTCCCTGGGCAGAGGAACTATTGACGATGTCAAATACATTGGCCGTGATCCATCAGCTGCCACAGCCACTGGTTTTTCTCAGGTTCATGATATGGAACAAAACGAGATTTTACAGAAAGCGATGCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004591 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005947 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0008150 GO:0008152 GO:0009060 GO:0009353 GO:0009987 GO:0015980 GO:0016491 GO:0016624 GO:0016903 GO:0016999 GO:0017144 GO:0019752 GO:0030062 GO:0031974 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045239 GO:0045240 GO:0045252 GO:0045333 GO:0055114 GO:0070013 GO:0071704 GO:0072350 GO:0098798 GO:1902494 GO:1990204 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00020 ko00310 ko00380 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00310 map00380 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

2-oxogl_dehyd_N E1_dh OxoGdeHyase_C Transket_pyr

Protein Analysis

1012

Amino Acids

114.35

Weight (kDa)

6.11

Isoelectric Point (pI)

38.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-oxogl_dehyd_N	PF16078	62 - 99	9.3e-14	2-oxoglutarate dehydrogenase N-terminus
E1_dh	PF00676	240 - 563	6.4e-59	Dehydrogenase E1 component
Transket_pyr	PF02779	633 - 846	2.2e-47	Transketolase, pyrimidine binding domain
OxoGdeHyase_C	PF16870	869 - 1010	6.2e-49	2-oxoglutarate dehydrogenase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001811)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55410 AT3G55410 AT5G65750
fragaria_vesca	FvH4_7g21770 FvH4_7g21770 FvH4_7g21770 FvH4_7g21770 FvH4_7g21770 FvH4_7g21770 FvH4_7g21851
malus_domestica	MD01G1122800.v1.1 MD02G1281300.v1.1 MD02G1281500.v1.1 MD07G1192300.v1.1
prunus_persica	Prupe.2G230300_v2.0.a1 Prupe.2G230400_v2.0.a1 Prupe.2G230400_v2.0.a1 Prupe.2G230400_v2.0.a1
pyrus_communis	pycom01g14980 pycom02g24050 pycom07g18180
rosa_chinensis	RchiOBHm_Chr1g0365921 RchiOBHm_Chr1g0366031
rosa_laevigata	RLG00000027377 RLG00000027378
rosa_multiflora	Rmu_co8265029.1_g000001 Rmu_sc0004288.1_g000005 Rmu_sc0006406.1_g000008
rosa_roxburghii	Rroxscaffold_4G00290620 Rroxscaffold_4G00290640
rosa_rugosa	Rorug01G0325800 Rorug01G0325900 Rorug01G0326000 Rorug01G0326100 Rorug01G0326200 Rorug01G0326300 Rorug01G0326400
rosa_samantha	Rh1AG334500 Rh1AG334600 Rh1BG295400 Rh1BG295600 Rh1CG312100 Rh1CG312200 Rh1DG327700 Rh1DG328000
rosa_wichuraiana	Rw1G029690 Rw1G029720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	559, 1971
Acc36I	ACCTGC	1 cut(s)	2419
AccB1I	GGYRCC	1 cut(s)	1440
AccB7I	CCANNNNNTGG	2 cut(s)	762, 1322
AccII	CGCG	1 cut(s)	1872
AciI	CCGC	5 cut(s)	916, 2069, 2497, 2887, 2898
AclWI	GGATC	2 cut(s)	76, 2948
AcoI	YGGCCR	2 cut(s)	753, 2947
AcsI	RAATTY	8 cut(s)	52, 289, 944, 1749, 2409, 2437, 2492, 2557
AcuI	CTGAAG	3 cut(s)	212, 1317, 2333
AcyI	GRCGYC	1 cut(s)	1441
AdeI	CACNNNGTG	2 cut(s)	762, 1282
AfeI	AGCGCT	1 cut(s)	1243
AfiI	CCNNNNNNNGG	8 cut(s)	762, 884, 1043, 1266, 1322, 2080, 2400, 2632
AgsI	TTSAA	9 cut(s)	341, 791, 1146, 1366, 1628, 1653, 1792, 1985, 2138
AhdI	GACNNNNNGTC	1 cut(s)	1318
AjiI	CACGTC	1 cut(s)	1153
AjnI	CCWGG	6 cut(s)	269, 378, 2025, 2307, 2590, 2910
AjuI	GAANNNNNNNTTGG	4 cut(s)	389, 421, 2175, 2207
Alw21I	GWGCWC	2 cut(s)	2215, 2332
Alw26I	GTCTC	8 cut(s)	105, 648, 659, 883, 1048, 1220, 1698, 1880
Alw44I	GTGCAC	1 cut(s)	2328
AlwI	GGATC	2 cut(s)	76, 2948
AlwNI	CAGNNNCTG	2 cut(s)	479, 2975
Ama87I	CYCGRG	1 cut(s)	848
Aor51HI	AGCGCT	1 cut(s)	1243
AoxI	GGCC	8 cut(s)	303, 328, 753, 784, 2305, 2311, 2584, 2947
ApaI	GGGCCC	1 cut(s)	2315
ApaLI	GTGCAC	1 cut(s)	2328
ApeKI	GCWGC	6 cut(s)	770, 839, 1197, 1430, 1528, 2963
ApoI	RAATTY	8 cut(s)	52, 289, 944, 1749, 2409, 2437, 2492, 2557
ArsI	GACNNNNNNTTYG	2 cut(s)	2354, 2386
AseI	ATTAAT	1 cut(s)	1940
Asp700I	GAANNNNTTC	2 cut(s)	1330, 2343
AspLEI	GCGC	5 cut(s)	354, 1244, 1443, 1874, 2625
AsuC2I	CCSGG	4 cut(s)	318, 574, 813, 2272
AsuHPI	GGTGA	6 cut(s)	1010, 1297, 1731, 1904, 2207, 2636
AsuII	TTCGAA	1 cut(s)	1552
AsuNHI	GCTAGC	1 cut(s)	1676
AvaI	CYCGRG	1 cut(s)	848
AvaII	GGWCC	9 cut(s)	409, 451, 561, 597, 1102, 1161, 2071, 2610, 2635
AxyI	CCTNAGG	1 cut(s)	557
BaeGI	GKGCMC	2 cut(s)	2315, 2332
BalI	TGGCCA	1 cut(s)	755
BanI	GGYRCC	1 cut(s)	1440
BanII	GRGCYC	2 cut(s)	2215, 2315
BbsI	GAAGAC	1 cut(s)	1626
Bbv12I	GWGCWC	2 cut(s)	2215, 2332
BbvI	GCAGC	6 cut(s)	782, 826, 1209, 1417, 1540, 2950
BceAI	ACGGC	3 cut(s)	2552, 2690, 2934
BcgI	CGANNNNNNTGC	2 cut(s)	1412, 1446
BciT130I	CCWGG	6 cut(s)	271, 380, 2027, 2309, 2592, 2912
BciVI	GTATCC	2 cut(s)	1626, 1769
BclI	TGATCA	1 cut(s)	2227
BcnI	CCSGG	4 cut(s)	318, 574, 813, 2272
BcoDI	GTCTC	8 cut(s)	105, 648, 659, 883, 1048, 1220, 1698, 1880
BfaI	CTAG	8 cut(s)	24, 81, 174, 1041, 1125, 1595, 1677, 2325
BfoI	RGCGCY	3 cut(s)	1245, 1444, 2626
BfuAI	ACCTGC	1 cut(s)	2419
BfuI	GTATCC	2 cut(s)	1626, 1769
BglII	AGATCT	1 cut(s)	1328
BisI	GCNGC	7 cut(s)	771, 840, 1198, 1431, 1529, 2898, 2964
BlsI	GCNGC	7 cut(s)	772, 841, 1199, 1432, 1530, 2899, 2965
Bme18I	GGWCC	9 cut(s)	409, 451, 561, 597, 1102, 1161, 2071, 2610, 2635
BmeRI	GACNNNNNGTC	1 cut(s)	1318
BmeT110I	CYCGRG	1 cut(s)	848
BmgBI	CACGTC	1 cut(s)	1153
BmrI	ACTGGG	2 cut(s)	999, 1779
BmsI	GCATC	3 cut(s)	1243, 1724, 3018
BmtI	GCTAGC	1 cut(s)	1680
BmuI	ACTGGG	2 cut(s)	999, 1779
BpiI	GAAGAC	1 cut(s)	1626
BpmI	CTGGAG	2 cut(s)	532, 2055
Bpu14I	TTCGAA	1 cut(s)	1552
BpuEI	CTTGAG	3 cut(s)	599, 2199, 2678
BpuMI	CCSGG	4 cut(s)	318, 574, 813, 2272
Bsa29I	ATCGAT	1 cut(s)	637
BsaBI	GATNNNNATC	2 cut(s)	633, 2364
BsaHI	GRCGYC	1 cut(s)	1441
BsaJI	CCNNGG	5 cut(s)	270, 2308, 2590, 2910, 2911
BsaXI	ACNNNNNCTCC	4 cut(s)	2202, 2232, 2240, 2270
Bsc4I	CCNNNNNNNGG	8 cut(s)	762, 884, 1043, 1266, 1322, 2080, 2400, 2632
Bse21I	CCTNAGG	1 cut(s)	557
Bse3DI	GCAATG	2 cut(s)	621, 2877
Bse8I	GATNNNNATC	2 cut(s)	633, 2364
BseBI	CCWGG	6 cut(s)	271, 380, 2027, 2309, 2592, 2912
BseCI	ATCGAT	1 cut(s)	637
BseDI	CCNNGG	5 cut(s)	270, 2308, 2590, 2910, 2911
BseGI	GGATG	9 cut(s)	202, 522, 976, 1516, 1558, 1715, 1984, 2749, 2793
BseJI	GATNNNNATC	2 cut(s)	633, 2364
BseLI	CCNNNNNNNGG	8 cut(s)	762, 884, 1043, 1266, 1322, 2080, 2400, 2632
BseMI	GCAATG	2 cut(s)	621, 2877
BseMII	CTCAG	7 cut(s)	416, 525, 1958, 2201, 2373, 2817, 2999
BseRI	GAGGAG	1 cut(s)	2675
BseSI	GKGCMC	2 cut(s)	2315, 2332
BseXI	GCAGC	6 cut(s)	782, 826, 1209, 1417, 1540, 2950
BseYI	CCCAGC	1 cut(s)	1564
BsgI	GTGCAG	2 cut(s)	569, 1587
Bsh1236I	CGCG	1 cut(s)	1872
Bsh1285I	CGRYCG	1 cut(s)	631
BshFI	GGCC	8 cut(s)	305, 330, 755, 786, 2307, 2313, 2586, 2949
BshNI	GGYRCC	1 cut(s)	1440
BshVI	ATCGAT	1 cut(s)	637
BsiEI	CGRYCG	1 cut(s)	631
BsiHKAI	GWGCWC	2 cut(s)	2215, 2332
BsiHKCI	CYCGRG	1 cut(s)	848
BsiSI	CCGG	6 cut(s)	318, 574, 813, 1532, 2271, 2691
BslFI	GGGAC	8 cut(s)	125, 287, 528, 610, 1006, 1282, 2623, 2893
BslI	CCNNNNNNNGG	8 cut(s)	762, 884, 1043, 1266, 1322, 2080, 2400, 2632
BsmAI	GTCTC	8 cut(s)	105, 648, 659, 883, 1048, 1220, 1698, 1880
BsmBI	CGTCTC	1 cut(s)	105
BsmFI	GGGAC	8 cut(s)	125, 287, 528, 610, 1006, 1282, 2623, 2893
BsmI	GAATGC	3 cut(s)	879, 1372, 2423
BsnI	GGCC	8 cut(s)	305, 330, 755, 786, 2307, 2313, 2586, 2949
BsoBI	CYCGRG	1 cut(s)	848
Bsp119I	TTCGAA	1 cut(s)	1552
Bsp120I	GGGCCC	1 cut(s)	2311
Bsp1286I	GDGCHC	3 cut(s)	2215, 2315, 2332
Bsp1407I	TGTACA	2 cut(s)	1343, 1539
Bsp143I	GATC	8 cut(s)	68, 481, 628, 844, 1328, 2227, 2653, 2953
BspACI	CCGC	5 cut(s)	916, 2069, 2497, 2887, 2898
BspANI	GGCC	8 cut(s)	305, 330, 755, 786, 2307, 2313, 2586, 2949
BspCNI	CTCAG	7 cut(s)	415, 526, 1959, 2200, 2374, 2818, 2998
BspDI	ATCGAT	1 cut(s)	637
BspFNI	CGCG	1 cut(s)	1872
BspHI	TCATGA	1 cut(s)	2992
BspMI	ACCTGC	1 cut(s)	2419
BspOI	GCTAGC	1 cut(s)	1680
BspPI	GGATC	2 cut(s)	76, 2948
BspQI	GCTCTTC	2 cut(s)	1251, 2841
BspT104I	TTCGAA	1 cut(s)	1552
BspT107I	GGYRCC	1 cut(s)	1440
BsrDI	GCAATG	2 cut(s)	621, 2877
BsrGI	TGTACA	2 cut(s)	1343, 1539
BssECI	CCNNGG	5 cut(s)	270, 2308, 2590, 2910, 2911
BssMI	GATC	8 cut(s)	68, 481, 628, 844, 1328, 2227, 2653, 2953
BssNI	GRCGYC	1 cut(s)	1441
Bst2UI	CCWGG	6 cut(s)	271, 380, 2027, 2309, 2592, 2912
Bst4CI	ACNGT	5 cut(s)	542, 738, 2088, 2095, 2105
Bst6I	CTCTTC	4 cut(s)	420, 1251, 1787, 2841
BstACI	GRCGYC	1 cut(s)	1441
BstAPI	GCANNNNNTGC	2 cut(s)	38, 1367
BstAUI	TGTACA	2 cut(s)	1343, 1539
BstBI	TTCGAA	1 cut(s)	1552
BstC8I	GCNNGC	8 cut(s)	39, 585, 1424, 1428, 1678, 1713, 2501, 2696
BstEII	GGTNACC	1 cut(s)	1952
BstENI	CCTNNNNNAGG	2 cut(s)	882, 2630
BstF5I	GGATG	9 cut(s)	202, 522, 976, 1516, 1558, 1715, 1984, 2749, 2793
BstFNI	CGCG	1 cut(s)	1872
BstH2I	RGCGCY	3 cut(s)	1245, 1444, 2626
BstHHI	GCGC	5 cut(s)	354, 1244, 1443, 1874, 2625
BstKTI	GATC	8 cut(s)	71, 484, 631, 847, 1331, 2230, 2656, 2956
BstMAI	GTCTC	8 cut(s)	105, 648, 659, 883, 1048, 1220, 1698, 1880
BstMBI	GATC	8 cut(s)	68, 481, 628, 844, 1328, 2227, 2653, 2953
BstMCI	CGRYCG	1 cut(s)	631
BstMWI	GCNNNNNNNGC	4 cut(s)	38, 143, 1367, 2969
BstNI	CCWGG	6 cut(s)	271, 380, 2027, 2309, 2592, 2912
BstPI	GGTNACC	1 cut(s)	1952
BstSLI	GKGCMC	2 cut(s)	2315, 2332
BstUI	CGCG	1 cut(s)	1872
BstV1I	GCAGC	6 cut(s)	782, 826, 1209, 1417, 1540, 2950
BstV2I	GAAGAC	1 cut(s)	1626
BstX2I	RGATCY	1 cut(s)	1328
BstXI	CCANNNNNNTGG	5 cut(s)	386, 1171, 1727, 1769, 2033
BstYI	RGATCY	1 cut(s)	1328
Bsu15I	ATCGAT	1 cut(s)	637
Bsu36I	CCTNAGG	1 cut(s)	557
BsuI	GTATCC	2 cut(s)	1626, 1769
BsuRI	GGCC	8 cut(s)	305, 330, 755, 786, 2307, 2313, 2586, 2949
BsuTUI	ATCGAT	1 cut(s)	637
BtrI	CACGTC	1 cut(s)	1153
BtsCI	GGATG	9 cut(s)	202, 522, 976, 1516, 1558, 1715, 1984, 2749, 2793
BtsI	GCAGTG	1 cut(s)	24
BtsIMutI	CAGTG	5 cut(s)	24, 955, 987, 1260, 2973
BveI	ACCTGC	1 cut(s)	2419
Cac8I	GCNNGC	8 cut(s)	39, 585, 1424, 1428, 1678, 1713, 2501, 2696
CaiI	CAGNNNCTG	2 cut(s)	479, 2975
CciI	TCATGA	1 cut(s)	2992
CfoI	GCGC	5 cut(s)	354, 1244, 1443, 1874, 2625
ClaI	ATCGAT	1 cut(s)	637
CseI	GACGC	2 cut(s)	917, 2462
CsiI	ACCWGGT	1 cut(s)	378
CspCI	CAANNNNNGTGG	4 cut(s)	948, 983, 1134, 1169
DinI	GGCGCC	1 cut(s)	1442
DpnI	GATC	8 cut(s)	70, 483, 630, 846, 1330, 2229, 2655, 2955
DpnII	GATC	8 cut(s)	68, 481, 628, 844, 1328, 2227, 2653, 2953
DraIII	CACNNNGTG	2 cut(s)	762, 1282
DrdI	GACNNNNNNGTC	2 cut(s)	559, 1971
DriI	GACNNNNNGTC	1 cut(s)	1318
DseDI	GACNNNNNNGTC	2 cut(s)	559, 1971
EaeI	YGGCCR	2 cut(s)	753, 2947
Eam1104I	CTCTTC	4 cut(s)	420, 1251, 1787, 2841
Eam1105I	GACNNNNNGTC	1 cut(s)	1318
EarI	CTCTTC	4 cut(s)	420, 1251, 1787, 2841
EciI	GGCGGA	1 cut(s)	2084
Ecl136II	GAGCTC	1 cut(s)	2213
Eco147I	AGGCCT	1 cut(s)	305
Eco24I	GRGCYC	2 cut(s)	2215, 2315
Eco32I	GATATC	2 cut(s)	436, 2816
Eco47I	GGWCC	9 cut(s)	409, 451, 561, 597, 1102, 1161, 2071, 2610, 2635
Eco47III	AGCGCT	1 cut(s)	1243
Eco53kI	GAGCTC	1 cut(s)	2213
Eco57I	CTGAAG	3 cut(s)	212, 1317, 2333
Eco81I	CCTNAGG	1 cut(s)	557
Eco88I	CYCGRG	1 cut(s)	848
Eco91I	GGTNACC	1 cut(s)	1952
EcoICRI	GAGCTC	1 cut(s)	2213
EcoNI	CCTNNNNNAGG	2 cut(s)	882, 2630
EcoO109I	RGGNCCY	2 cut(s)	2311, 2312
EcoO65I	GGTNACC	1 cut(s)	1952
EcoRI	GAATTC	2 cut(s)	944, 2492
EcoRII	CCWGG	6 cut(s)	269, 378, 2025, 2307, 2590, 2910
EcoRV	GATATC	2 cut(s)	436, 2816
EcoT38I	GRGCYC	2 cut(s)	2215, 2315
EgeI	GGCGCC	1 cut(s)	1442
EheI	GGCGCC	1 cut(s)	1442
Esp3I	CGTCTC	1 cut(s)	105
FalI	AAGNNNNNCTT	2 cut(s)	998, 1030
FaqI	GGGAC	8 cut(s)	125, 287, 528, 610, 1006, 1282, 2623, 2893
FauI	CCCGC	1 cut(s)	2894
FbaI	TGATCA	1 cut(s)	2227
Fnu4HI	GCNGC	7 cut(s)	771, 840, 1198, 1431, 1529, 2898, 2964
FokI	GGATG	9 cut(s)	209, 529, 983, 1523, 1545, 1702, 1991, 2756, 2780
FriOI	GRGCYC	2 cut(s)	2215, 2315
Fsp4HI	GCNGC	7 cut(s)	771, 840, 1198, 1431, 1529, 2898, 2964
FspBI	CTAG	8 cut(s)	24, 81, 174, 1041, 1125, 1595, 1677, 2325
GlaI	GCGC	5 cut(s)	353, 1243, 1442, 1873, 2624
GluI	GCNGC	7 cut(s)	771, 840, 1198, 1431, 1529, 2898, 2964
GsaI	CCCAGC	1 cut(s)	1568
GsuI	CTGGAG	2 cut(s)	532, 2055
HaeII	RGCGCY	3 cut(s)	1245, 1444, 2626
HaeIII	GGCC	8 cut(s)	305, 330, 755, 786, 2307, 2313, 2586, 2949
HapII	CCGG	6 cut(s)	318, 574, 813, 1532, 2271, 2691
HgaI	GACGC	2 cut(s)	917, 2462
HhaI	GCGC	5 cut(s)	354, 1244, 1443, 1874, 2625
Hin1I	GRCGYC	1 cut(s)	1441
Hin6I	GCGC	5 cut(s)	352, 1242, 1441, 1872, 2623
HinP1I	GCGC	5 cut(s)	352, 1242, 1441, 1872, 2623
HindIII	AAGCTT	2 cut(s)	35, 2696
HpaII	CCGG	6 cut(s)	318, 574, 813, 1532, 2271, 2691
HphI	GGTGA	6 cut(s)	1010, 1297, 1731, 1904, 2207, 2636
Hpy166II	GTNNAC	9 cut(s)	471, 734, 1102, 1156, 1222, 2020, 2091, 2108, 2330
Hpy8I	GTNNAC	9 cut(s)	471, 734, 1102, 1156, 1222, 2020, 2091, 2108, 2330
Hpy99I	CGWCG	3 cut(s)	702, 1491, 2478
HpyAV	CCTTC	9 cut(s)	785, 814, 1312, 1829, 1849, 1889, 1943, 2540, 2788
HpyCH4III	ACNGT	5 cut(s)	542, 738, 2088, 2095, 2105
HpyCH4IV	ACGT	2 cut(s)	697, 1152
HpyF10VI	GCNNNNNNNGC	4 cut(s)	38, 143, 1367, 2969
HpySE526I	ACGT	2 cut(s)	697, 1152
Hsp92I	GRCGYC	1 cut(s)	1441
HspAI	GCGC	5 cut(s)	352, 1242, 1441, 1872, 2623
KasI	GGCGCC	1 cut(s)	1440
Ksp22I	TGATCA	1 cut(s)	2227
Kzo9I	GATC	8 cut(s)	68, 481, 628, 844, 1328, 2227, 2653, 2953
LguI	GCTCTTC	2 cut(s)	1251, 2841
LmnI	GCTCC	5 cut(s)	1375, 2210, 2524, 2693, 2735
Lsp1109I	GCAGC	6 cut(s)	782, 826, 1209, 1417, 1540, 2950
LweI	GCATC	3 cut(s)	1243, 1724, 3018
MabI	ACCWGGT	1 cut(s)	378
MaeI	CTAG	8 cut(s)	24, 81, 174, 1041, 1125, 1595, 1677, 2325
MaeII	ACGT	2 cut(s)	697, 1152
MaeIII	GTNAC	9 cut(s)	389, 641, 1085, 1609, 1737, 1810, 1952, 2144, 2297
MalI	GATC	8 cut(s)	70, 483, 630, 846, 1330, 2229, 2655, 2955
MboI	GATC	8 cut(s)	68, 481, 628, 844, 1328, 2227, 2653, 2953
MfeI	CAATTG	1 cut(s)	2422
MflI	RGATCY	1 cut(s)	1328
MhlI	GDGCHC	3 cut(s)	2215, 2315, 2332
MlsI	TGGCCA	1 cut(s)	755
MluNI	TGGCCA	1 cut(s)	755
Mly113I	GGCGCC	1 cut(s)	1441
MlyI	GAGTC	5 cut(s)	94, 275, 661, 1222, 2258
MmeI	TCCRAC	4 cut(s)	387, 695, 1096, 2713
Mox20I	TGGCCA	1 cut(s)	755
MroXI	GAANNNNTTC	2 cut(s)	1330, 2343
MscI	TGGCCA	1 cut(s)	755
MseI	TTAA	6 cut(s)	939, 1647, 1734, 1833, 1940, 2619
MslI	CAYNNNNRTG	5 cut(s)	1191, 1283, 1386, 1458, 2325
Msp20I	TGGCCA	1 cut(s)	755
MspA1I	CMGCKG	1 cut(s)	2963
MspI	CCGG	6 cut(s)	318, 574, 813, 1532, 2271, 2691
MunI	CAATTG	1 cut(s)	2422
Mva1269I	GAATGC	3 cut(s)	879, 1372, 2423
MvaI	CCWGG	6 cut(s)	271, 380, 2027, 2309, 2592, 2912
MvnI	CGCG	1 cut(s)	1872
MwoI	GCNNNNNNNGC	4 cut(s)	38, 143, 1367, 2969
NarI	GGCGCC	1 cut(s)	1441
NciI	CCSGG	4 cut(s)	318, 574, 813, 2272
NdeII	GATC	8 cut(s)	68, 481, 628, 844, 1328, 2227, 2653, 2953
NheI	GCTAGC	1 cut(s)	1676
NmuCI	GTSAC	5 cut(s)	389, 1085, 1609, 1737, 1810
NspV	TTCGAA	1 cut(s)	1552
PagI	TCATGA	1 cut(s)	2992
PasI	CCCWGGG	1 cut(s)	2911
PceI	AGGCCT	1 cut(s)	305
PciSI	GCTCTTC	2 cut(s)	1251, 2841
PcsI	WCGNNNNNNNCGW	1 cut(s)	2338
PctI	GAATGC	3 cut(s)	879, 1372, 2423
PdmI	GAANNNNTTC	2 cut(s)	1330, 2343
PfeI	GAWTC	5 cut(s)	151, 1183, 1598, 2390, 2641
PflFI	GACNNNGTC	1 cut(s)	2933
PflMI	CCANNNNNTGG	2 cut(s)	762, 1322
PkrI	GCNGC	7 cut(s)	772, 841, 1199, 1432, 1530, 2899, 2965
Ple19I	CGATCG	1 cut(s)	631
PleI	GAGTC	5 cut(s)	93, 274, 661, 1222, 2257
PluTI	GGCGCC	1 cut(s)	1444
PpsI	GAGTC	5 cut(s)	93, 274, 661, 1222, 2257
PshBI	ATTAAT	1 cut(s)	1940
Psp124BI	GAGCTC	1 cut(s)	2215
Psp6I	CCWGG	6 cut(s)	269, 378, 2025, 2307, 2590, 2910
PspEI	GGTNACC	1 cut(s)	1952
PspFI	CCCAGC	1 cut(s)	1564
PspGI	CCWGG	6 cut(s)	269, 378, 2025, 2307, 2590, 2910
PspOMI	GGGCCC	1 cut(s)	2311
PstNI	CAGNNNCTG	2 cut(s)	479, 2975
PsuI	RGATCY	1 cut(s)	1328
PsyI	GACNNNGTC	1 cut(s)	2933
PvuI	CGATCG	1 cut(s)	631
PvuII	CAGCTG	1 cut(s)	2963
RseI	CAYNNNNRTG	5 cut(s)	1191, 1283, 1386, 1458, 2325
SacI	GAGCTC	1 cut(s)	2215
SapI	GCTCTTC	2 cut(s)	1251, 2841
SaqAI	TTAA	6 cut(s)	939, 1647, 1734, 1833, 1940, 2619
SatI	GCNGC	7 cut(s)	771, 840, 1198, 1431, 1529, 2898, 2964
Sau3AI	GATC	8 cut(s)	68, 481, 628, 844, 1328, 2227, 2653, 2953
SchI	GAGTC	5 cut(s)	94, 275, 661, 1222, 2258
SduI	GDGCHC	3 cut(s)	2215, 2315, 2332
SexAI	ACCWGGT	1 cut(s)	378
SfaNI	GCATC	3 cut(s)	1243, 1724, 3018
SfoI	GGCGCC	1 cut(s)	1442
SfuI	TTCGAA	1 cut(s)	1552
SinI	GGWCC	9 cut(s)	409, 451, 561, 597, 1102, 1161, 2071, 2610, 2635
SmiMI	CAYNNNNRTG	5 cut(s)	1191, 1283, 1386, 1458, 2325
SmlI	CTYRAG	3 cut(s)	578, 2214, 2657
SmoI	CTYRAG	3 cut(s)	578, 2214, 2657
SseBI	AGGCCT	1 cut(s)	305
SsiI	CCGC	5 cut(s)	916, 2069, 2497, 2887, 2898
SspDI	GGCGCC	1 cut(s)	1440
SspI	AATATT	1 cut(s)	2062
SspMI	CTAG	8 cut(s)	24, 81, 174, 1041, 1125, 1595, 1677, 2325
SstI	GAGCTC	1 cut(s)	2215
StuI	AGGCCT	1 cut(s)	305
TaaI	ACNGT	5 cut(s)	542, 738, 2088, 2095, 2105
TaiI	ACGT	2 cut(s)	700, 1155
TaqI	TCGA	6 cut(s)	637, 1489, 1552, 1963, 2150, 2571
TatI	WGTACW	4 cut(s)	684, 731, 1343, 1539
TauI	GCSGC	1 cut(s)	2900
TfiI	GAWTC	5 cut(s)	151, 1183, 1598, 2390, 2641
Tru1I	TTAA	6 cut(s)	939, 1647, 1734, 1833, 1940, 2619
Tru9I	TTAA	6 cut(s)	939, 1647, 1734, 1833, 1940, 2619
TscAI	CASTG	5 cut(s)	24, 955, 994, 1267, 2980
TseFI	GTSAC	5 cut(s)	389, 1085, 1609, 1737, 1810
TseI	GCWGC	6 cut(s)	770, 839, 1197, 1430, 1528, 2963
Tsp45I	GTSAC	5 cut(s)	389, 1085, 1609, 1737, 1810
TspGWI	ACGGA	1 cut(s)	87
TspRI	CASTG	5 cut(s)	24, 955, 994, 1267, 2980
Tth111I	GACNNNGTC	1 cut(s)	2933
Van91I	CCANNNNNTGG	2 cut(s)	762, 1322
VneI	GTGCAC	1 cut(s)	2328
VpaK11BI	GGWCC	9 cut(s)	409, 451, 561, 597, 1102, 1161, 2071, 2610, 2635
VspI	ATTAAT	1 cut(s)	1940
XagI	CCTNNNNNAGG	2 cut(s)	882, 2630
XapI	RAATTY	8 cut(s)	52, 289, 944, 1749, 2409, 2437, 2492, 2557
XbaI	TCTAGA	2 cut(s)	173, 1594
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2908
XmnI	GAANNNNTTC	2 cut(s)	1330, 2343
XspI	CTAG	8 cut(s)	24, 81, 174, 1041, 1125, 1595, 1677, 2325

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.