MD01G1126200.v1.1

Anaphase-promoting complex subunit 11 RING-H2 finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004301
Physical Location & Seq
Forward (+)
23806138 .. 23806554
417 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1126200.v1.1.491

Sequence Viewer

Length: 417 bp
ATGAATTCCTTGTTCACGTCTGACCCCTTGACTTTTCTACTTGCTTTCGGCGGGTGTTTGCTTGCGATGATCCTCATTGGTATATGTTTCAGTGGCCGGAAAATCGCGCAAGAAGAACTGACACGGCGTCGTCTGGGTCCTGTAATGCATGAGGCGCCGGCAGCAAGCAGCTTGGAAGCCCAAGTCAATTTGGCCCAACAGAAGTGTCGAAAGGTTGTGGGCCTAGAGTTGGTCGGAGATGGCACGTGTGCCGTGTGCCTAGGCGAGTACGAAGAAGGCGAAAATTATCGTACCTTGCCGGAGTGCAACCACTCGTTTCACGTTCCATGCATTGAAATGTGGCTCTATTACCATACGACTTGCCCCGTTTGTCGGGCCAGTGCAACGCCGTTGACGGTAGTCGTTCAACATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

139

Amino Acids

15.2

Weight (kDa)

5.91

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 82 - 125 1.4e-13 Ring finger domain
zf-RING_11 PF17123 83 - 111 3.8e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 83 - 124 1.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 83 - 124 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 89 - 125 1.5e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 154
AccII CGCG 1 cut(s) 107
AciI CCGC 1 cut(s) 51
AclWI GGATC 1 cut(s) 64
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 1 cut(s) 4
AcvI CACGTG 1 cut(s) 246
AcyI GRCGYC 2 cut(s) 127, 155
AfaI GTAC 2 cut(s) 269, 292
AfiI CCNNNNNNNGG 2 cut(s) 229, 372
AflIII ACRYGT 1 cut(s) 245
AgsI TTSAA 2 cut(s) 335, 407
AhdI GACNNNNNGTC 1 cut(s) 126
AjiI CACGTC 1 cut(s) 18
AluBI AGCT 1 cut(s) 171
AluI AGCT 1 cut(s) 171
AlwI GGATC 1 cut(s) 64
AoxI GGCC 4 cut(s) 94, 192, 220, 375
ApeKI GCWGC 2 cut(s) 161, 168
ApoI RAATTY 1 cut(s) 4
AspA2I CCTAGG 1 cut(s) 259
AspLEI GCGC 2 cut(s) 109, 157
AspS9I GGNCC 4 cut(s) 137, 193, 220, 375
AvaII GGWCC 1 cut(s) 137
AvrII CCTAGG 1 cut(s) 259
BanI GGYRCC 1 cut(s) 154
BbrPI CACGTG 1 cut(s) 246
BbvI GCAGC 2 cut(s) 173, 180
BccI CCATC 1 cut(s) 233
BceAI ACGGC 3 cut(s) 140, 236, 373
BfaI CTAG 2 cut(s) 224, 260
BfoI RGCGCY 1 cut(s) 158
BisI GCNGC 2 cut(s) 162, 169
BlnI CCTAGG 1 cut(s) 259
BlsI GCNGC 2 cut(s) 163, 170
Bme18I GGWCC 1 cut(s) 137
BmeRI GACNNNNNGTC 1 cut(s) 126
BmgBI CACGTC 1 cut(s) 18
BmgT120I GGNCC 4 cut(s) 137, 193, 220, 375
BmiI GGNNCC 2 cut(s) 138, 156
BoxI GACNNNNGTC 1 cut(s) 398
BsaAI YACGTR 1 cut(s) 246
BsaHI GRCGYC 2 cut(s) 127, 155
BsaJI CCNNGG 1 cut(s) 259
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 372
Bse118I RCCGGY 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 378
BseDI CCNNGG 1 cut(s) 259
BseLI CCNNNNNNNGG 2 cut(s) 229, 372
BseNI ACTGG 1 cut(s) 378
BseXI GCAGC 2 cut(s) 173, 180
Bsh1236I CGCG 1 cut(s) 107
BshFI GGCC 4 cut(s) 96, 194, 222, 377
BshNI GGYRCC 1 cut(s) 154
BsiSI CCGG 3 cut(s) 97, 158, 299
BslI CCNNNNNNNGG 2 cut(s) 229, 372
BsnI GGCC 4 cut(s) 96, 194, 222, 377
Bsp143I GATC 1 cut(s) 69
BspACI CCGC 1 cut(s) 51
BspANI GGCC 4 cut(s) 96, 194, 222, 377
BspFNI CGCG 1 cut(s) 107
BspLI GGNNCC 2 cut(s) 138, 156
BspPI GGATC 1 cut(s) 64
BspT107I GGYRCC 1 cut(s) 154
BsrFI RCCGGY 1 cut(s) 157
BsrI ACTGG 1 cut(s) 378
BssAI RCCGGY 1 cut(s) 157
BssECI CCNNGG 1 cut(s) 259
BssMI GATC 1 cut(s) 69
BssNI GRCGYC 2 cut(s) 127, 155
BssT1I CCWWGG 1 cut(s) 259
Bst4CI ACNGT 1 cut(s) 397
BstACI GRCGYC 2 cut(s) 127, 155
BstBAI YACGTR 1 cut(s) 246
BstC8I GCNNGC 3 cut(s) 63, 159, 166
BstFNI CGCG 1 cut(s) 107
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 2 cut(s) 109, 157
BstKTI GATC 1 cut(s) 72
BstMBI GATC 1 cut(s) 69
BstMWI GCNNNNNNNGC 2 cut(s) 154, 161
BstPAI GACNNNNGTC 1 cut(s) 398
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 2 cut(s) 173, 180
BsuRI GGCC 4 cut(s) 96, 194, 222, 377
BtgZI GCGATG 1 cut(s) 80
BtrI CACGTC 1 cut(s) 18
BtsIMutI CAGTG 2 cut(s) 97, 385
Cac8I GCNNGC 3 cut(s) 63, 159, 166
CfoI GCGC 2 cut(s) 109, 157
Cfr10I RCCGGY 1 cut(s) 157
Cfr13I GGNCC 4 cut(s) 137, 193, 220, 375
CseI GACGC 1 cut(s) 116
Csp6I GTAC 2 cut(s) 268, 291
CviAII CATG 2 cut(s) 149, 327
CviJI RGCY 7 cut(s) 96, 171, 179, 194, 222, 343, 377
CviKI_1 RGCY 7 cut(s) 96, 171, 179, 194, 222, 343, 377
CviQI GTAC 2 cut(s) 268, 291
DinI GGCGCC 1 cut(s) 156
DpnI GATC 1 cut(s) 71
DpnII GATC 1 cut(s) 69
DriI GACNNNNNGTC 1 cut(s) 126
EaeI YGGCCR 1 cut(s) 94
Eam1105I GACNNNNNGTC 1 cut(s) 126
Eco130I CCWWGG 1 cut(s) 259
Eco47I GGWCC 1 cut(s) 137
Eco72I CACGTG 1 cut(s) 246
EcoO109I RGGNCCY 1 cut(s) 137
EcoRI GAATTC 1 cut(s) 4
EcoT14I CCWWGG 1 cut(s) 259
EcoT22I ATGCAT 2 cut(s) 150, 332
EgeI GGCGCC 1 cut(s) 156
EheI GGCGCC 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 259
FaeI CATG 2 cut(s) 152, 330
FaiI YATR 5 cut(s) 83, 85, 150, 328, 354
FatI CATG 2 cut(s) 148, 326
FauI CCCGC 1 cut(s) 44
Fnu4HI GCNGC 2 cut(s) 162, 169
Fsp4HI GCNGC 2 cut(s) 162, 169
FspBI CTAG 2 cut(s) 224, 260
GlaI GCGC 2 cut(s) 108, 156
GluI GCNGC 2 cut(s) 162, 169
HaeII RGCGCY 1 cut(s) 158
HaeIII GGCC 4 cut(s) 96, 194, 222, 377
HapII CCGG 3 cut(s) 97, 158, 299
HgaI GACGC 1 cut(s) 116
HhaI GCGC 2 cut(s) 109, 157
Hin1I GRCGYC 2 cut(s) 127, 155
Hin1II CATG 2 cut(s) 152, 330
Hin6I GCGC 2 cut(s) 107, 155
HinP1I GCGC 2 cut(s) 107, 155
HincII GTYRAC 1 cut(s) 393
HindII GTYRAC 1 cut(s) 393
HpaII CCGG 3 cut(s) 97, 158, 299
Hpy166II GTNNAC 2 cut(s) 15, 393
Hpy188I TCNGA 2 cut(s) 22, 236
Hpy8I GTNNAC 2 cut(s) 15, 393
Hpy99I CGWCG 1 cut(s) 132
HpyAV CCTTC 1 cut(s) 269
HpyCH4III ACNGT 1 cut(s) 397
HpyCH4IV ACGT 3 cut(s) 17, 245, 321
HpyCH4V TGCA 4 cut(s) 148, 306, 330, 383
HpyF10VI GCNNNNNNNGC 2 cut(s) 154, 161
HpySE526I ACGT 3 cut(s) 17, 245, 321
Hsp92I GRCGYC 2 cut(s) 127, 155
Hsp92II CATG 2 cut(s) 152, 330
HspAI GCGC 2 cut(s) 107, 155
KasI GGCGCC 1 cut(s) 154
KroI GCCGGC 1 cut(s) 157
KroNI GCCGGC 1 cut(s) 159
Kzo9I GATC 1 cut(s) 69
LpnPI CCDG 6 cut(s) 110, 119, 153, 171, 312, 391
Lsp1109I GCAGC 2 cut(s) 173, 180
MaeI CTAG 2 cut(s) 224, 260
MaeII ACGT 3 cut(s) 17, 245, 321
MalI GATC 1 cut(s) 71
MboI GATC 1 cut(s) 69
MboII GAAGA 2 cut(s) 125, 284
MluCI AATT 3 cut(s) 4, 187, 283
Mly113I GGCGCC 1 cut(s) 155
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 2 cut(s) 83, 145
Mph1103I ATGCAT 2 cut(s) 150, 332
MroNI GCCGGC 1 cut(s) 157
MslI CAYNNNNRTG 1 cut(s) 335
MspI CCGG 3 cut(s) 97, 158, 299
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 2 cut(s) 154, 161
NaeI GCCGGC 1 cut(s) 159
NarI GGCGCC 1 cut(s) 155
NdeII GATC 1 cut(s) 69
NgoMIV GCCGGC 1 cut(s) 157
NlaIII CATG 2 cut(s) 152, 330
NlaIV GGNNCC 2 cut(s) 138, 156
NsiI ATGCAT 2 cut(s) 150, 332
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PdiI GCCGGC 1 cut(s) 159
PkrI GCNGC 2 cut(s) 163, 170
PluTI GGCGCC 1 cut(s) 158
PmaCI CACGTG 1 cut(s) 246
PmlI CACGTG 1 cut(s) 246
Ppu21I YACGTR 1 cut(s) 246
PpuMI RGGWCCY 1 cut(s) 137
PshAI GACNNNNGTC 1 cut(s) 398
Psp5II RGGWCCY 1 cut(s) 137
PspCI CACGTG 1 cut(s) 246
PspN4I GGNNCC 2 cut(s) 138, 156
PspPI GGNCC 4 cut(s) 137, 193, 220, 375
PspPPI RGGWCCY 1 cut(s) 137
RsaI GTAC 2 cut(s) 269, 292
RsaNI GTAC 2 cut(s) 268, 291
RseI CAYNNNNRTG 1 cut(s) 335
SatI GCNGC 2 cut(s) 162, 169
Sau3AI GATC 1 cut(s) 69
Sau96I GGNCC 4 cut(s) 137, 193, 220, 375
SetI ASST 6 cut(s) 20, 173, 216, 248, 296, 324
SfoI GGCGCC 1 cut(s) 156
SinI GGWCC 1 cut(s) 137
SmiMI CAYNNNNRTG 1 cut(s) 335
Sse9I AATT 3 cut(s) 4, 187, 283
SsiI CCGC 1 cut(s) 51
SspDI GGCGCC 1 cut(s) 154
SspMI CTAG 2 cut(s) 224, 260
StyI CCWWGG 1 cut(s) 259
TaaI ACNGT 1 cut(s) 397
TaiI ACGT 3 cut(s) 20, 248, 324
TaqI TCGA 1 cut(s) 208
TasI AATT 3 cut(s) 4, 187, 283
TscAI CASTG 2 cut(s) 97, 385
TseI GCWGC 2 cut(s) 161, 168
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 97, 385
VpaK11BI GGWCC 1 cut(s) 137
XapI RAATTY 1 cut(s) 4
XmaJI CCTAGG 1 cut(s) 259
XspI CTAG 2 cut(s) 224, 260
Zsp2I ATGCAT 2 cut(s) 150, 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.