Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0004301

Physical Location & Seq

Genomic Coordinates Forward (+)

27465960 .. 27466433

Gene Structure 474 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1196100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 474 bp

ATGGCTGAAAGCCATAACCTTGTCTCTGATCCCAGATTATACATTCTTCTAGTTGCTCTGAGCTCGGCTGTGCTTGTTGTCACACTCTACCATTGCGCAGTCATTTGTTGCTGCAACCGCAACCCCCAACCACGACAAACACGACGCCGTTTGAGGCCCGAAACCACGGGGCTCACAGATGGCAGCATGGGGCCTTCATCGGCATCCGAACTCATTCCGTCCAGGAAGTATCAAAGGGACGTTGGCTTGGTCTTGGTGGGAGACGGTATGTGTGCTGTGTGCATAAGTGATTTTGAAGAAGGTGAAGAGTTTCGTACCTTGCCGGAATGCATGCACTCATTTCACGTTTCATGCATTGACATGTGGCTGTTTTCTCATTCAAGTTGCCCGATTTGCCGTGCCGACGCCACACTCTCATCCCTCGTAGTTCGGTGTTTGCCTGATTTAGAGTCCGGTAACGTAGAGAGCCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

158

Amino Acids

17.33

Weight (kDa)

5.69

Isoelectric Point (pI)

61.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	90 - 133	2.1e-12	Ring finger domain
zf-C3HC4_2	PF13923	90 - 132	1.6e-07	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	91 - 119	3.4e-09	RING-like zinc finger
zf-rbx1	PF12678	99 - 133	1e-06	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05280
fragaria_vesca	FvH4_1g08250
malus_domestica	MD01G1126200.v1.1 MD02G1086800.v1.1 MD07G1195700.v1.1 MD07G1195800.v1.1 MD07G1196000.v1.1 MD07G1196100.v1.1 MD15G1214000.v1.1
prunus_persica	Prupe.2G233100_v2.0.a1 Prupe.3G043300_v2.0.a1 Prupe.7G203000_v2.0.a1
pyrus_communis	pycom01g15190 pycom09g10280 pycom15g19020
rosa_chinensis	RchiOBHm_Chr2g0094301 RchiOBHm_Chr2g0144911
rosa_laevigata	RLG00000016475 RLG00000020118
rosa_multiflora	Rmu_sc0002354.1_g000011 Rmu_sc0003819.1_g000026
rosa_roxburghii	Rroxscaffold_2G00147280
rosa_rugosa	Rorug02G0042800
rosa_samantha	Rh2AG090600 Rh2AG442000 Rh2BG090500 Rh2BG452600 Rh2CG093000 Rh2CG429300 Rh2DG089500 Rh2DG462500
rosa_wichuraiana	Rw2G036140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	97
AciI	CCGC	1 cut(s)	118
AclWI	GGATC	1 cut(s)	23
AcyI	GRCGYC	2 cut(s)	145, 405
AfaI	GTAC	1 cut(s)	316
AflIII	ACRYGT	1 cut(s)	360
AgsI	TTSAA	2 cut(s)	296, 381
AjnI	CCWGG	1 cut(s)	221
AluBI	AGCT	1 cut(s)	63
AluI	AGCT	1 cut(s)	63
Alw21I	GWGCWC	1 cut(s)	65
Alw26I	GTCTC	2 cut(s)	28, 255
AlwI	GGATC	1 cut(s)	23
AoxI	GGCC	2 cut(s)	155, 191
ApeKI	GCWGC	2 cut(s)	111, 183
Asp700I	GAANNNNTTC	2 cut(s)	213, 309
AspLEI	GCGC	1 cut(s)	98
AspS9I	GGNCC	2 cut(s)	156, 191
AsuHPI	GGTGA	1 cut(s)	314
BanII	GRGCYC	2 cut(s)	65, 174
Bbv12I	GWGCWC	1 cut(s)	65
BbvI	GCAGC	2 cut(s)	98, 195
BccI	CCATC	1 cut(s)	173
BceAI	ACGGC	2 cut(s)	132, 381
BciT130I	CCWGG	1 cut(s)	223
BcoDI	GTCTC	2 cut(s)	28, 255
BfaI	CTAG	1 cut(s)	50
BisI	GCNGC	2 cut(s)	112, 184
BlsI	GCNGC	2 cut(s)	113, 185
Bme1390I	CCNGG	1 cut(s)	223
BmgT120I	GGNCC	2 cut(s)	156, 191
BmiI	GGNNCC	1 cut(s)	192
BmrFI	CCNGG	1 cut(s)	223
BmsI	GCATC	1 cut(s)	212
BsaHI	GRCGYC	2 cut(s)	145, 405
BsaJI	CCNNGG	1 cut(s)	165
BsaWI	WCCGGW	1 cut(s)	452
Bse3DI	GCAATG	1 cut(s)	91
BseBI	CCWGG	1 cut(s)	223
BseDI	CCNNGG	1 cut(s)	165
BseGI	GGATG	2 cut(s)	203, 416
BseMI	GCAATG	1 cut(s)	91
BseMII	CTCAG	1 cut(s)	50
BseXI	GCAGC	2 cut(s)	98, 195
BshFI	GGCC	2 cut(s)	157, 193
BsiHKAI	GWGCWC	1 cut(s)	65
BsiSI	CCGG	2 cut(s)	323, 453
BslFI	GGGAC	1 cut(s)	251
BsmAI	GTCTC	2 cut(s)	28, 255
BsmBI	CGTCTC	1 cut(s)	255
BsmFI	GGGAC	1 cut(s)	251
BsmI	GAATGC	1 cut(s)	332
BsnI	GGCC	2 cut(s)	157, 193
Bsp1286I	GDGCHC	2 cut(s)	65, 174
Bsp143I	GATC	1 cut(s)	28
BspACI	CCGC	1 cut(s)	118
BspANI	GGCC	2 cut(s)	157, 193
BspCNI	CTCAG	1 cut(s)	51
BspLI	GGNNCC	1 cut(s)	192
BspPI	GGATC	1 cut(s)	23
BsrDI	GCAATG	1 cut(s)	91
BssECI	CCNNGG	1 cut(s)	165
BssMI	GATC	1 cut(s)	28
BssNI	GRCGYC	2 cut(s)	145, 405
Bst2UI	CCWGG	1 cut(s)	223
Bst4CI	ACNGT	1 cut(s)	266
Bst6I	CTCTTC	1 cut(s)	300
BstACI	GRCGYC	2 cut(s)	145, 405
BstC8I	GCNNGC	1 cut(s)	332
BstDEI	CTNAG	1 cut(s)	59
BstDSI	CCRYGG	1 cut(s)	165
BstF5I	GGATG	2 cut(s)	203, 416
BstHHI	GCGC	1 cut(s)	98
BstKTI	GATC	1 cut(s)	31
BstMAI	GTCTC	2 cut(s)	28, 255
BstMBI	GATC	1 cut(s)	28
BstMWI	GCNNNNNNNGC	2 cut(s)	117, 393
BstNI	CCWGG	1 cut(s)	223
BstNSI	RCATGY	2 cut(s)	334, 364
BstSCI	CCNGG	1 cut(s)	221
BstV1I	GCAGC	2 cut(s)	98, 195
BsuRI	GGCC	2 cut(s)	157, 193
BtgI	CCRYGG	1 cut(s)	165
BtsCI	GGATG	2 cut(s)	203, 416
Cac8I	GCNNGC	1 cut(s)	332
CfoI	GCGC	1 cut(s)	98
Cfr13I	GGNCC	2 cut(s)	156, 191
CseI	GACGC	2 cut(s)	153, 413
Csp6I	GTAC	1 cut(s)	315
CviAII	CATG	4 cut(s)	187, 331, 351, 361
CviQI	GTAC	1 cut(s)	315
DdeI	CTNAG	1 cut(s)	59
DpnI	GATC	1 cut(s)	30
DpnII	GATC	1 cut(s)	28
Eam1104I	CTCTTC	1 cut(s)	300
EarI	CTCTTC	1 cut(s)	300
Ecl136II	GAGCTC	1 cut(s)	63
Eco24I	GRGCYC	2 cut(s)	65, 174
Eco53kI	GAGCTC	1 cut(s)	63
EcoICRI	GAGCTC	1 cut(s)	63
EcoO109I	RGGNCCY	1 cut(s)	191
EcoRII	CCWGG	1 cut(s)	221
EcoT22I	ATGCAT	2 cut(s)	332, 356
EcoT38I	GRGCYC	2 cut(s)	65, 174
Esp3I	CGTCTC	1 cut(s)	255
FaeI	CATG	4 cut(s)	190, 334, 354, 364
FaiI	YATR	8 cut(s)	15, 40, 188, 269, 284, 332, 352, 362
FaqI	GGGAC	1 cut(s)	251
FatI	CATG	4 cut(s)	186, 330, 350, 360
Fnu4HI	GCNGC	2 cut(s)	112, 184
FokI	GGATG	2 cut(s)	190, 403
FriOI	GRGCYC	2 cut(s)	65, 174
Fsp4HI	GCNGC	2 cut(s)	112, 184
FspBI	CTAG	1 cut(s)	50
FspI	TGCGCA	1 cut(s)	97
GlaI	GCGC	1 cut(s)	97
GluI	GCNGC	2 cut(s)	112, 184
HaeIII	GGCC	2 cut(s)	157, 193
HapII	CCGG	2 cut(s)	323, 453
HgaI	GACGC	2 cut(s)	153, 413
HhaI	GCGC	1 cut(s)	98
Hin1I	GRCGYC	2 cut(s)	145, 405
Hin1II	CATG	4 cut(s)	190, 334, 354, 364
Hin6I	GCGC	1 cut(s)	96
HinP1I	GCGC	1 cut(s)	96
HinfI	GANTC	1 cut(s)	449
HpaII	CCGG	2 cut(s)	323, 453
HphI	GGTGA	1 cut(s)	314
Hpy188I	TCNGA	3 cut(s)	28, 60, 208
Hpy99I	CGWCG	2 cut(s)	147, 407
HpyAV	CCTTC	2 cut(s)	204, 293
HpyCH4III	ACNGT	1 cut(s)	266
HpyCH4IV	ACGT	3 cut(s)	240, 345, 459
HpyCH4V	TGCA	5 cut(s)	114, 282, 330, 334, 354
HpyF10VI	GCNNNNNNNGC	2 cut(s)	117, 393
HpyF3I	CTNAG	1 cut(s)	59
HpySE526I	ACGT	3 cut(s)	240, 345, 459
Hsp92I	GRCGYC	2 cut(s)	145, 405
Hsp92II	CATG	4 cut(s)	190, 334, 354, 364
HspAI	GCGC	1 cut(s)	96
Kzo9I	GATC	1 cut(s)	28
LpnPI	CCDG	6 cut(s)	46, 208, 235, 336, 453, 466
Lsp1109I	GCAGC	2 cut(s)	98, 195
LweI	GCATC	1 cut(s)	212
MaeI	CTAG	1 cut(s)	50
MaeII	ACGT	3 cut(s)	240, 345, 459
MaeIII	GTNAC	2 cut(s)	79, 455
MalI	GATC	1 cut(s)	30
MboI	GATC	1 cut(s)	28
MboII	GAAGA	3 cut(s)	38, 308, 317
MhlI	GDGCHC	2 cut(s)	65, 174
MlyI	GAGTC	1 cut(s)	458
MnlI	CCTC	2 cut(s)	147, 431
Mph1103I	ATGCAT	2 cut(s)	332, 356
MroXI	GAANNNNTTC	2 cut(s)	213, 309
MslI	CAYNNNNRTG	1 cut(s)	359
MspI	CCGG	2 cut(s)	323, 453
MspR9I	CCNGG	1 cut(s)	223
Mva1269I	GAATGC	1 cut(s)	332
MvaI	CCWGG	1 cut(s)	223
MwoI	GCNNNNNNNGC	2 cut(s)	117, 393
NdeII	GATC	1 cut(s)	28
NlaIII	CATG	4 cut(s)	190, 334, 354, 364
NlaIV	GGNNCC	1 cut(s)	192
NmeAIII	GCCGAG	1 cut(s)	44
NmuCI	GTSAC	1 cut(s)	79
NsbI	TGCGCA	1 cut(s)	97
NsiI	ATGCAT	2 cut(s)	332, 356
NspI	RCATGY	2 cut(s)	334, 364
PaeI	GCATGC	1 cut(s)	334
PciI	ACATGT	1 cut(s)	360
PcsI	WCGNNNNNNNCGW	1 cut(s)	139
PctI	GAATGC	1 cut(s)	332
PdmI	GAANNNNTTC	2 cut(s)	213, 309
PfoI	TCCNGGA	1 cut(s)	221
PkrI	GCNGC	2 cut(s)	113, 185
PleI	GAGTC	1 cut(s)	457
PpsI	GAGTC	1 cut(s)	457
PscI	ACATGT	1 cut(s)	360
Psp124BI	GAGCTC	1 cut(s)	65
Psp6I	CCWGG	1 cut(s)	221
PspGI	CCWGG	1 cut(s)	221
PspN4I	GGNNCC	1 cut(s)	192
PspPI	GGNCC	2 cut(s)	156, 191
RsaI	GTAC	1 cut(s)	316
RsaNI	GTAC	1 cut(s)	315
RseI	CAYNNNNRTG	1 cut(s)	359
SacI	GAGCTC	1 cut(s)	65
SatI	GCNGC	2 cut(s)	112, 184
Sau3AI	GATC	1 cut(s)	28
Sau96I	GGNCC	2 cut(s)	156, 191
SchI	GAGTC	1 cut(s)	458
ScrFI	CCNGG	1 cut(s)	223
SduI	GDGCHC	2 cut(s)	65, 174
SetI	ASST	7 cut(s)	21, 65, 243, 304, 320, 348, 462
SfaNI	GCATC	1 cut(s)	212
SmiMI	CAYNNNNRTG	1 cut(s)	359
SphI	GCATGC	1 cut(s)	334
SsiI	CCGC	1 cut(s)	118
SspMI	CTAG	1 cut(s)	50
SstI	GAGCTC	1 cut(s)	65
StyD4I	CCNGG	1 cut(s)	221
TaaI	ACNGT	1 cut(s)	266
TaiI	ACGT	3 cut(s)	243, 348, 462
TseFI	GTSAC	1 cut(s)	79
TseI	GCWGC	2 cut(s)	111, 183
Tsp45I	GTSAC	1 cut(s)	79
TspDTI	ATGAA	2 cut(s)	186, 339
TspGWI	ACGGA	1 cut(s)	207
XceI	RCATGY	2 cut(s)	334, 364
XmnI	GAANNNNTTC	2 cut(s)	213, 309
XspI	CTAG	1 cut(s)	50
Zsp2I	ATGCAT	2 cut(s)	332, 356

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.