MD07G1196000.v1.1

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004301
Physical Location & Seq
Forward (+)
27463345 .. 27463758
414 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1196000.v1.1.491

Sequence Viewer

Length: 414 bp
ATGGTCTCCGGAGAGTCTGAATATGGTCCTGGACTTTTTCTCATCATTCCGCTTGTGGTGATCATAGCCATCTGGTGGTGTTGCAGTTGCTGCTTCGAGAATATTCCCCATGAAGAACCGACACAACGACAATTGGAAGATGATGAAATGCAAAAGACCACAGCAGCAAGCGGTACAAGGACTACTACCGCTTCACCGGTAATCCAGAAGTATGAAAAGGGTGTTAGCCTGGTAGGAGATGGTACGTGTCCTGTGTGCATAGAAAAATTTAAAGAAGGTGAATATTTTCGTACCTTGCCGGAGTGCAAGCACTCGTTTCACGCTGCATGCATTGACATGTGGCTCTATTCTCATCCGAATTGCCCAGTTTGCCGCGCCACTGCTACACAGTTCACGGTTGTTGTTCAGCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

138

Amino Acids

15.35

Weight (kDa)

5.3

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-ANAPC11 PF12861 80 - 128 5.6e-07 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-rbx1 PF12678 81 - 125 6.1e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 82 - 125 2.9e-13 Ring finger domain
zf-C3HC4_2 PF13923 83 - 124 1.4e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 83 - 111 1.4e-08 RING-like zinc finger
zf-C3HC4 PF00097 83 - 124 2.3e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 75
AccII CGCG 1 cut(s) 375
AccIII TCCGGA 1 cut(s) 8
AciI CCGC 5 cut(s) 50, 171, 189, 373, 409
AcsI RAATTY 1 cut(s) 266
AfaI GTAC 3 cut(s) 175, 244, 292
AfiI CCNNNNNNNGG 1 cut(s) 75
AflIII ACRYGT 2 cut(s) 245, 336
AgeI ACCGGT 1 cut(s) 196
AjnI CCWGG 2 cut(s) 28, 228
Alw26I GTCTC 1 cut(s) 10
AlwNI CAGNNNCTG 1 cut(s) 90
Aor13HI TCCGGA 1 cut(s) 8
ApeKI GCWGC 3 cut(s) 90, 164, 323
ApoI RAATTY 1 cut(s) 266
AsiGI ACCGGT 1 cut(s) 196
Asp700I GAANNNNTTC 1 cut(s) 285
AspLEI GCGC 1 cut(s) 377
AspS9I GGNCC 1 cut(s) 26
AsuHPI GGTGA 3 cut(s) 70, 186, 290
AvaII GGWCC 1 cut(s) 26
BbvI GCAGC 3 cut(s) 77, 176, 310
BccI CCATC 2 cut(s) 77, 233
BciT130I CCWGG 2 cut(s) 30, 230
BclI TGATCA 1 cut(s) 60
BcoDI GTCTC 1 cut(s) 10
BisI GCNGC 4 cut(s) 91, 165, 324, 373
BlsI GCNGC 4 cut(s) 92, 166, 325, 374
Bme1390I CCNGG 2 cut(s) 30, 230
Bme18I GGWCC 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 26
BmrFI CCNGG 2 cut(s) 30, 230
BmrI ACTGGG 1 cut(s) 359
BmuI ACTGGG 1 cut(s) 359
BsaAI YACGTR 1 cut(s) 246
BsaI GGTCTC 1 cut(s) 10
BsaWI WCCGGW 2 cut(s) 8, 196
Bsc4I CCNNNNNNNGG 1 cut(s) 75
Bse118I RCCGGY 1 cut(s) 196
Bse1I ACTGG 1 cut(s) 365
BseAI TCCGGA 1 cut(s) 8
BseBI CCWGG 2 cut(s) 30, 230
BseGI GGATG 1 cut(s) 352
BseLI CCNNNNNNNGG 1 cut(s) 75
BseNI ACTGG 1 cut(s) 365
BseXI GCAGC 3 cut(s) 77, 176, 310
Bsh1236I CGCG 1 cut(s) 375
BshTI ACCGGT 1 cut(s) 196
BsiSI CCGG 3 cut(s) 9, 197, 299
BslI CCNNNNNNNGG 1 cut(s) 75
BsmAI GTCTC 1 cut(s) 10
Bso31I GGTCTC 1 cut(s) 10
Bsp13I TCCGGA 1 cut(s) 8
Bsp143I GATC 1 cut(s) 60
BspACI CCGC 5 cut(s) 50, 171, 189, 373, 409
BspEI TCCGGA 1 cut(s) 8
BspFNI CGCG 1 cut(s) 375
BspTNI GGTCTC 1 cut(s) 10
BsrFI RCCGGY 1 cut(s) 196
BsrI ACTGG 1 cut(s) 365
BssAI RCCGGY 1 cut(s) 196
BssMI GATC 1 cut(s) 60
Bst2UI CCWGG 2 cut(s) 30, 230
Bst4CI ACNGT 2 cut(s) 390, 397
BstAPI GCANNNNNTGC 1 cut(s) 90
BstBAI YACGTR 1 cut(s) 246
BstC8I GCNNGC 3 cut(s) 169, 308, 328
BstF5I GGATG 1 cut(s) 352
BstFNI CGCG 1 cut(s) 375
BstHHI GCGC 1 cut(s) 377
BstKTI GATC 1 cut(s) 63
BstMAI GTCTC 1 cut(s) 10
BstMBI GATC 1 cut(s) 60
BstMWI GCNNNNNNNGC 2 cut(s) 90, 369
BstNI CCWGG 2 cut(s) 30, 230
BstNSI RCATGY 2 cut(s) 330, 340
BstSCI CCNGG 2 cut(s) 28, 228
BstUI CGCG 1 cut(s) 375
BstV1I GCAGC 3 cut(s) 77, 176, 310
BtsCI GGATG 1 cut(s) 352
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 1 cut(s) 378
Cac8I GCNNGC 3 cut(s) 169, 308, 328
CaiI CAGNNNCTG 1 cut(s) 90
CfoI GCGC 1 cut(s) 377
Cfr10I RCCGGY 1 cut(s) 196
Cfr13I GGNCC 1 cut(s) 26
Csp6I GTAC 3 cut(s) 174, 243, 291
CspAI ACCGGT 1 cut(s) 196
CviAII CATG 3 cut(s) 110, 327, 337
CviJI RGCY 3 cut(s) 68, 228, 343
CviKI_1 RGCY 3 cut(s) 68, 228, 343
CviQI GTAC 3 cut(s) 174, 243, 291
DpnI GATC 1 cut(s) 62
DpnII GATC 1 cut(s) 60
DraI TTTAAA 1 cut(s) 271
Eco31I GGTCTC 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 26
EcoRII CCWGG 2 cut(s) 28, 228
EcoT22I ATGCAT 1 cut(s) 332
FaeI CATG 3 cut(s) 113, 330, 340
FaiI YATR 7 cut(s) 24, 65, 111, 213, 260, 328, 338
FatI CATG 3 cut(s) 109, 326, 336
FbaI TGATCA 1 cut(s) 60
Fnu4HI GCNGC 4 cut(s) 91, 165, 324, 373
FokI GGATG 1 cut(s) 339
Fsp4HI GCNGC 4 cut(s) 91, 165, 324, 373
GlaI GCGC 1 cut(s) 376
GluI GCNGC 4 cut(s) 91, 165, 324, 373
HapII CCGG 3 cut(s) 9, 197, 299
HhaI GCGC 1 cut(s) 377
Hin1II CATG 3 cut(s) 113, 330, 340
Hin6I GCGC 1 cut(s) 375
HinP1I GCGC 1 cut(s) 375
HinfI GANTC 1 cut(s) 14
HpaII CCGG 3 cut(s) 9, 197, 299
HphI GGTGA 3 cut(s) 70, 186, 290
Hpy166II GTNNAC 1 cut(s) 393
Hpy188I TCNGA 2 cut(s) 19, 357
Hpy188III TCNNGA 3 cut(s) 9, 97, 205
Hpy8I GTNNAC 1 cut(s) 393
HpyAV CCTTC 1 cut(s) 269
HpyCH4III ACNGT 2 cut(s) 390, 397
HpyCH4IV ACGT 1 cut(s) 245
HpyCH4V TGCA 6 cut(s) 84, 151, 258, 306, 326, 330
HpyF10VI GCNNNNNNNGC 2 cut(s) 90, 369
HpySE526I ACGT 1 cut(s) 245
Hsp92II CATG 3 cut(s) 113, 330, 340
HspAI GCGC 1 cut(s) 375
Kpn2I TCCGGA 1 cut(s) 8
Ksp22I TGATCA 1 cut(s) 60
Kzo9I GATC 1 cut(s) 60
Lsp1109I GCAGC 3 cut(s) 77, 176, 310
MaeII ACGT 1 cut(s) 245
MalI GATC 1 cut(s) 62
MboI GATC 1 cut(s) 60
MboII GAAGA 2 cut(s) 125, 149
MfeI CAATTG 1 cut(s) 131
MluCI AATT 3 cut(s) 131, 266, 358
MlyI GAGTC 1 cut(s) 23
Mph1103I ATGCAT 1 cut(s) 332
MroI TCCGGA 1 cut(s) 8
MroXI GAANNNNTTC 1 cut(s) 285
MseI TTAA 1 cut(s) 270
MslI CAYNNNNRTG 1 cut(s) 335
MspA1I CMGCKG 1 cut(s) 409
MspI CCGG 3 cut(s) 9, 197, 299
MspR9I CCNGG 2 cut(s) 30, 230
MunI CAATTG 1 cut(s) 131
MvaI CCWGG 2 cut(s) 30, 230
MvnI CGCG 1 cut(s) 375
MwoI GCNNNNNNNGC 2 cut(s) 90, 369
NdeII GATC 1 cut(s) 60
NlaIII CATG 3 cut(s) 113, 330, 340
NsiI ATGCAT 1 cut(s) 332
NspI RCATGY 2 cut(s) 330, 340
PaeI GCATGC 1 cut(s) 330
PciI ACATGT 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 285
PflMI CCANNNNNTGG 1 cut(s) 75
PfoI TCCNGGA 1 cut(s) 28
PinAI ACCGGT 1 cut(s) 196
PkrI GCNGC 4 cut(s) 92, 166, 325, 374
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
Ppu21I YACGTR 1 cut(s) 246
PscI ACATGT 1 cut(s) 336
Psp6I CCWGG 2 cut(s) 28, 228
PspGI CCWGG 2 cut(s) 28, 228
PspPI GGNCC 1 cut(s) 26
PstNI CAGNNNCTG 1 cut(s) 90
RsaI GTAC 3 cut(s) 175, 244, 292
RsaNI GTAC 3 cut(s) 174, 243, 291
RseI CAYNNNNRTG 1 cut(s) 335
SaqAI TTAA 1 cut(s) 270
SatI GCNGC 4 cut(s) 91, 165, 324, 373
Sau3AI GATC 1 cut(s) 60
Sau96I GGNCC 1 cut(s) 26
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 2 cut(s) 30, 230
SetI ASST 3 cut(s) 248, 280, 296
SinI GGWCC 1 cut(s) 26
SmiMI CAYNNNNRTG 1 cut(s) 335
SphI GCATGC 1 cut(s) 330
Sse9I AATT 3 cut(s) 131, 266, 358
SsiI CCGC 5 cut(s) 50, 171, 189, 373, 409
SspI AATATT 2 cut(s) 103, 284
StyD4I CCNGG 2 cut(s) 28, 228
TaaI ACNGT 2 cut(s) 390, 397
TaiI ACGT 1 cut(s) 248
TaqI TCGA 1 cut(s) 96
TasI AATT 3 cut(s) 131, 266, 358
TauI GCSGC 1 cut(s) 375
Tru1I TTAA 1 cut(s) 270
Tru9I TTAA 1 cut(s) 270
TscAI CASTG 1 cut(s) 385
TseI GCWGC 3 cut(s) 90, 164, 323
TspDTI ATGAA 3 cut(s) 126, 159, 228
TspRI CASTG 1 cut(s) 385
Van91I CCANNNNNTGG 1 cut(s) 75
VpaK11BI GGWCC 1 cut(s) 26
XapI RAATTY 1 cut(s) 266
XceI RCATGY 2 cut(s) 330, 340
XmnI GAANNNNTTC 1 cut(s) 285
Zsp2I ATGCAT 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.