pycom01g15190

Anaphase-promoting complex subunit 11 RING-H2 finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004301
Physical Location & Seq
Forward (+)
15138329 .. 15138774
446 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g15190.1

Sequence Viewer

Length: 372 bp
ATGGCCATTTTAGCCATTTCAAAGCCCGCCTCAAAGTTGAAGACCATGAATTTCTTGTTAACGTCTGAACCCTTGGATTTTCTACTTGCTTTCGGCCTGTGTGGGCTTGCGATGATCTGCATTGGTCATGAGGCCCCGGCAGTAAGCAGCTTGGAAGCCCAAACCAATTTGGCCCAACAGAAGTGTCGAAAGGTTGTGGGCCTAGAGTTGGTCCGAGATGGCACGTGTGCCGTGTGCCTAGGCAAGTACGAAGAAGGCGAAAATTATCGTACCTTGCCGGAGTGCAACCACTCGTTTCACGTTCCATGCATTAACATGTGGCTCTATTCTCATCCGACTTGCCCGGTTTGTCGGGCCAGTGCAACGCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

124

Amino Acids

13.4

Weight (kDa)

6.87

Isoelectric Point (pI)

28.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 75 - 118 1e-13 Ring finger domain
zf-RING_11 PF17123 76 - 104 5.2e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 76 - 117 5e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 76 - 117 9.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 77 - 118 6e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 27
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 49
AcvI CACGTG 1 cut(s) 225
AfaI GTAC 2 cut(s) 248, 271
AfiI CCNNNNNNNGG 1 cut(s) 208
AflIII ACRYGT 2 cut(s) 224, 315
AgsI TTSAA 2 cut(s) 21, 40
AluBI AGCT 1 cut(s) 150
AluI AGCT 1 cut(s) 150
AoxI GGCC 6 cut(s) 3, 94, 132, 171, 199, 354
ApeKI GCWGC 1 cut(s) 147
ApoI RAATTY 1 cut(s) 49
AspA2I CCTAGG 1 cut(s) 238
AspS9I GGNCC 5 cut(s) 133, 172, 199, 211, 354
AsuC2I CCSGG 2 cut(s) 137, 344
AvaII GGWCC 1 cut(s) 211
AvrII CCTAGG 1 cut(s) 238
BalI TGGCCA 1 cut(s) 5
BbrPI CACGTG 1 cut(s) 225
BbsI GAAGAC 1 cut(s) 47
BbvI GCAGC 1 cut(s) 159
BccI CCATC 1 cut(s) 212
BceAI ACGGC 2 cut(s) 215, 352
BcnI CCSGG 2 cut(s) 137, 344
BfaI CTAG 2 cut(s) 203, 239
BisI GCNGC 1 cut(s) 148
BlnI CCTAGG 1 cut(s) 238
BlsI GCNGC 1 cut(s) 149
Bme1390I CCNGG 2 cut(s) 137, 344
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 5 cut(s) 133, 172, 199, 211, 354
BmiI GGNNCC 1 cut(s) 135
BmrFI CCNGG 2 cut(s) 137, 344
BpiI GAAGAC 1 cut(s) 47
BpuMI CCSGG 2 cut(s) 137, 344
BsaAI YACGTR 1 cut(s) 225
BsaJI CCNNGG 3 cut(s) 72, 135, 238
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse1I ACTGG 1 cut(s) 357
BseDI CCNNGG 3 cut(s) 72, 135, 238
BseGI GGATG 1 cut(s) 331
BseLI CCNNNNNNNGG 1 cut(s) 208
BseNI ACTGG 1 cut(s) 357
BseXI GCAGC 1 cut(s) 159
BshFI GGCC 6 cut(s) 5, 96, 134, 173, 201, 356
BsiSI CCGG 3 cut(s) 137, 278, 344
BslI CCNNNNNNNGG 1 cut(s) 208
BsnI GGCC 6 cut(s) 5, 96, 134, 173, 201, 356
Bsp143I GATC 1 cut(s) 114
BspACI CCGC 1 cut(s) 27
BspANI GGCC 6 cut(s) 5, 96, 134, 173, 201, 356
BspHI TCATGA 1 cut(s) 127
BspLI GGNNCC 1 cut(s) 135
BsrI ACTGG 1 cut(s) 357
BssECI CCNNGG 3 cut(s) 72, 135, 238
BssMI GATC 1 cut(s) 114
BssT1I CCWWGG 2 cut(s) 72, 238
BstBAI YACGTR 1 cut(s) 225
BstC8I GCNNGC 2 cut(s) 27, 108
BstF5I GGATG 1 cut(s) 331
BstKTI GATC 1 cut(s) 117
BstMBI GATC 1 cut(s) 114
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 319
BstSCI CCNGG 2 cut(s) 135, 342
BstV1I GCAGC 1 cut(s) 159
BstV2I GAAGAC 1 cut(s) 47
BsuRI GGCC 6 cut(s) 5, 96, 134, 173, 201, 356
BtgZI GCGATG 1 cut(s) 125
BtsCI GGATG 1 cut(s) 331
BtsIMutI CAGTG 1 cut(s) 364
Cac8I GCNNGC 2 cut(s) 27, 108
CciI TCATGA 1 cut(s) 127
Cfr13I GGNCC 5 cut(s) 133, 172, 199, 211, 354
Csp6I GTAC 2 cut(s) 247, 270
CviAII CATG 4 cut(s) 46, 128, 306, 316
CviQI GTAC 2 cut(s) 247, 270
DpnI GATC 1 cut(s) 116
DpnII GATC 1 cut(s) 114
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 2 cut(s) 72, 238
Eco47I GGWCC 1 cut(s) 211
Eco72I CACGTG 1 cut(s) 225
EcoO109I RGGNCCY 1 cut(s) 133
EcoT14I CCWWGG 2 cut(s) 72, 238
EcoT22I ATGCAT 1 cut(s) 311
ErhI CCWWGG 2 cut(s) 72, 238
FaeI CATG 4 cut(s) 49, 131, 309, 319
FaiI YATR 4 cut(s) 47, 129, 307, 317
FatI CATG 4 cut(s) 45, 127, 305, 315
FauI CCCGC 1 cut(s) 34
Fnu4HI GCNGC 1 cut(s) 148
FokI GGATG 1 cut(s) 318
Fsp4HI GCNGC 1 cut(s) 148
FspBI CTAG 2 cut(s) 203, 239
GluI GCNGC 1 cut(s) 148
HaeIII GGCC 6 cut(s) 5, 96, 134, 173, 201, 356
HapII CCGG 3 cut(s) 137, 278, 344
Hin1II CATG 4 cut(s) 49, 131, 309, 319
HincII GTYRAC 1 cut(s) 60
HindII GTYRAC 1 cut(s) 60
HpaI GTTAAC 1 cut(s) 60
HpaII CCGG 3 cut(s) 137, 278, 344
Hpy166II GTNNAC 1 cut(s) 60
Hpy188I TCNGA 3 cut(s) 67, 215, 336
Hpy188III TCNNGA 1 cut(s) 128
Hpy8I GTNNAC 1 cut(s) 60
HpyAV CCTTC 1 cut(s) 248
HpyCH4IV ACGT 3 cut(s) 62, 224, 300
HpyCH4V TGCA 4 cut(s) 120, 285, 309, 362
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpySE526I ACGT 3 cut(s) 62, 224, 300
Hsp92II CATG 4 cut(s) 49, 131, 309, 319
KspAI GTTAAC 1 cut(s) 60
Kzo9I GATC 1 cut(s) 114
LpnPI CCDG 4 cut(s) 110, 150, 291, 357
Lsp1109I GCAGC 1 cut(s) 159
MaeI CTAG 2 cut(s) 203, 239
MaeII ACGT 3 cut(s) 62, 224, 300
MalI GATC 1 cut(s) 116
MboI GATC 1 cut(s) 114
MboII GAAGA 2 cut(s) 52, 263
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 49, 166, 262
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 359
MnlI CCTC 2 cut(s) 40, 124
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 311
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 59, 312
MslI CAYNNNNRTG 1 cut(s) 314
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 137, 278, 344
MspR9I CCNGG 2 cut(s) 137, 344
MwoI GCNNNNNNNGC 1 cut(s) 11
NciI CCSGG 2 cut(s) 137, 344
NdeII GATC 1 cut(s) 114
NlaIII CATG 4 cut(s) 49, 131, 309, 319
NlaIV GGNNCC 1 cut(s) 135
NsiI ATGCAT 1 cut(s) 311
NspI RCATGY 1 cut(s) 319
PagI TCATGA 1 cut(s) 127
PciI ACATGT 1 cut(s) 315
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PkrI GCNGC 1 cut(s) 149
PmaCI CACGTG 1 cut(s) 225
PmlI CACGTG 1 cut(s) 225
Ppu21I YACGTR 1 cut(s) 225
PscI ACATGT 1 cut(s) 315
PspCI CACGTG 1 cut(s) 225
PspN4I GGNNCC 1 cut(s) 135
PspPI GGNCC 5 cut(s) 133, 172, 199, 211, 354
RsaI GTAC 2 cut(s) 248, 271
RsaNI GTAC 2 cut(s) 247, 270
RseI CAYNNNNRTG 1 cut(s) 314
SaqAI TTAA 2 cut(s) 59, 312
SatI GCNGC 1 cut(s) 148
Sau3AI GATC 1 cut(s) 114
Sau96I GGNCC 5 cut(s) 133, 172, 199, 211, 354
ScrFI CCNGG 2 cut(s) 137, 344
SetI ASST 6 cut(s) 65, 152, 195, 227, 275, 303
SinI GGWCC 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 314
Sse9I AATT 3 cut(s) 49, 166, 262
SsiI CCGC 1 cut(s) 27
SspMI CTAG 2 cut(s) 203, 239
StyD4I CCNGG 2 cut(s) 135, 342
StyI CCWWGG 2 cut(s) 72, 238
TaiI ACGT 3 cut(s) 65, 227, 303
TaqI TCGA 1 cut(s) 187
TasI AATT 3 cut(s) 49, 166, 262
Tru1I TTAA 2 cut(s) 59, 312
Tru9I TTAA 2 cut(s) 59, 312
TscAI CASTG 1 cut(s) 364
TseI GCWGC 1 cut(s) 147
TspDTI ATGAA 1 cut(s) 62
TspRI CASTG 1 cut(s) 364
VpaK11BI GGWCC 1 cut(s) 211
XapI RAATTY 1 cut(s) 49
XceI RCATGY 1 cut(s) 319
XmaJI CCTAGG 1 cut(s) 238
XspI CTAG 2 cut(s) 203, 239
Zsp2I ATGCAT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.