MD02G1086800.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004301
Physical Location & Seq
Reverse (-)
6796256 .. 6797063
808 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1086800.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGGTTACAAGCTCCACACAATACAAAGCCCTTTTTCACAGCCACCACAGCCCCCGCCTACTCCGCCACCAAAAACCAACTTACCCATGTTCTATTACGGCCTCATAATCGTCTCAATAGCCGCCATAATCCTAGCCATGTACAACCTTATCTTCGTCAAACTGACTTCGAACCGCCACGATCAGTCTCCGCCACCAAGATCATCAATCAGTTTGGTTGATCTCTCAAGAACAAGGAGGAGTAGGAGCTTTGGGAACTTGGACAGCTTCAGGTACAAGAAGAACGAAGGGTCAACGTCAAAAGATGAAAGTTGTGTCGAATGTGCTGTTTGCTTGTCAGTTTTTGAGGACGGAGAAGAAATAAGAAAGCTTCCTACTTGCAAGCACTCCTTTCATGCTCCGTGCATAGACATGTGGCTCAACTCTCACTCTGACTGCCCGCTCTGTCGCACTCCCCTGCCGGCGAGCTCATGGAGTCATCCGCAGCTGACTACTACGCCGGAGGAGAATTCCAGAGAAGGAAGTCCTGCTACTGTGAGCTTTCTAGGGTTTCCTTTTTTCGGCTTTTTTTTTAATTTCCATATCTAG

Protein Analysis

196

Amino Acids

21.96

Weight (kDa)

8.18

Isoelectric Point (pI)

70.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 107 - 150 1.7e-13 Ring finger domain
zf-RING_11 PF17123 107 - 136 3.1e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 107 - 149 1.6e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 108 - 149 3.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 116 - 150 2e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 442
AciI CCGC 7 cut(s) 56, 65, 123, 175, 191, 440, 482
AcsI RAATTY 1 cut(s) 508
AcuI CTGAAG 1 cut(s) 253
AfaI GTAC 2 cut(s) 143, 275
AfiI CCNNNNNNNGG 1 cut(s) 560
AflIII ACRYGT 1 cut(s) 411
AluBI AGCT 7 cut(s) 13, 249, 267, 370, 468, 487, 540
AluI AGCT 7 cut(s) 13, 249, 267, 370, 468, 487, 540
Alw21I GWGCWC 1 cut(s) 470
Alw26I GTCTC 2 cut(s) 118, 192
AoxI GGCC 1 cut(s) 100
ApeKI GCWGC 1 cut(s) 484
ApoI RAATTY 1 cut(s) 508
AsuII TTCGAA 1 cut(s) 170
BanII GRGCYC 1 cut(s) 470
BarI GAAGNNNNNNTAC 2 cut(s) 514, 546
Bbv12I GWGCWC 1 cut(s) 470
BbvI GCAGC 1 cut(s) 496
BceAI ACGGC 1 cut(s) 115
BcoDI GTCTC 2 cut(s) 118, 192
BfaI CTAG 3 cut(s) 134, 545, 586
BisI GCNGC 2 cut(s) 123, 485
BlsI GCNGC 2 cut(s) 124, 486
Bpu14I TTCGAA 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 211
Bsc4I CCNNNNNNNGG 1 cut(s) 560
Bse118I RCCGGY 1 cut(s) 460
BseGI GGATG 1 cut(s) 478
BseLI CCNNNNNNNGG 1 cut(s) 560
BseRI GAGGAG 2 cut(s) 253, 518
BseXI GCAGC 1 cut(s) 496
BshFI GGCC 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 470
BsiSI CCGG 2 cut(s) 461, 500
BslI CCNNNNNNNGG 1 cut(s) 560
BsmAI GTCTC 2 cut(s) 118, 192
BsmBI CGTCTC 1 cut(s) 118
BsnI GGCC 1 cut(s) 102
Bsp119I TTCGAA 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 470
Bsp1407I TGTACA 1 cut(s) 141
Bsp143I GATC 3 cut(s) 181, 200, 220
BspACI CCGC 7 cut(s) 56, 65, 123, 175, 191, 440, 482
BspANI GGCC 1 cut(s) 102
BspT104I TTCGAA 1 cut(s) 170
BsrBI CCGCTC 1 cut(s) 442
BsrFI RCCGGY 1 cut(s) 460
BsrGI TGTACA 1 cut(s) 141
BssAI RCCGGY 1 cut(s) 460
BssMI GATC 3 cut(s) 181, 200, 220
Bst4CI ACNGT 1 cut(s) 535
BstAUI TGTACA 1 cut(s) 141
BstBI TTCGAA 1 cut(s) 170
BstC8I GCNNGC 4 cut(s) 383, 440, 462, 466
BstF5I GGATG 1 cut(s) 478
BstKTI GATC 3 cut(s) 184, 203, 223
BstMAI GTCTC 2 cut(s) 118, 192
BstMBI GATC 3 cut(s) 181, 200, 220
BstMWI GCNNNNNNNGC 2 cut(s) 49, 64
BstNSI RCATGY 1 cut(s) 415
BstV1I GCAGC 1 cut(s) 496
BsuRI GGCC 1 cut(s) 102
BtsCI GGATG 1 cut(s) 478
Cac8I GCNNGC 4 cut(s) 383, 440, 462, 466
Cfr10I RCCGGY 1 cut(s) 460
Csp6I GTAC 2 cut(s) 142, 274
CviAII CATG 5 cut(s) 88, 139, 395, 412, 471
CviQI GTAC 2 cut(s) 142, 274
DpnI GATC 3 cut(s) 183, 202, 222
DpnII GATC 3 cut(s) 181, 200, 220
EciI GGCGGA 2 cut(s) 54, 180
Ecl136II GAGCTC 1 cut(s) 468
Eco24I GRGCYC 1 cut(s) 470
Eco53kI GAGCTC 1 cut(s) 468
Eco57I CTGAAG 1 cut(s) 253
EcoICRI GAGCTC 1 cut(s) 468
EcoRI GAATTC 1 cut(s) 508
EcoT38I GRGCYC 1 cut(s) 470
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 5 cut(s) 91, 142, 398, 415, 474
FaiI YATR 9 cut(s) 89, 107, 128, 140, 396, 407, 413, 472, 582
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FatI CATG 5 cut(s) 87, 138, 394, 411, 470
FauI CCCGC 2 cut(s) 63, 447
Fnu4HI GCNGC 2 cut(s) 123, 485
FokI GGATG 1 cut(s) 465
FriOI GRGCYC 1 cut(s) 470
Fsp4HI GCNGC 2 cut(s) 123, 485
FspBI CTAG 3 cut(s) 134, 545, 586
GluI GCNGC 2 cut(s) 123, 485
HaeIII GGCC 1 cut(s) 102
HapII CCGG 2 cut(s) 461, 500
Hin1II CATG 5 cut(s) 91, 142, 398, 415, 474
HincII GTYRAC 1 cut(s) 294
HindII GTYRAC 1 cut(s) 294
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 1 cut(s) 475
HpaII CCGG 2 cut(s) 461, 500
Hpy166II GTNNAC 1 cut(s) 294
Hpy188I TCNGA 1 cut(s) 433
Hpy188III TCNNGA 2 cut(s) 228, 513
Hpy8I GTNNAC 1 cut(s) 294
HpyAV CCTTC 2 cut(s) 281, 512
HpyCH4III ACNGT 1 cut(s) 535
HpyCH4IV ACGT 1 cut(s) 296
HpyCH4V TGCA 2 cut(s) 381, 405
HpyF10VI GCNNNNNNNGC 2 cut(s) 49, 64
HpySE526I ACGT 1 cut(s) 296
Hsp92II CATG 5 cut(s) 91, 142, 398, 415, 474
KroI GCCGGC 1 cut(s) 460
KroNI GCCGGC 1 cut(s) 462
Kzo9I GATC 3 cut(s) 181, 200, 220
LmnI GCTCC 3 cut(s) 18, 246, 403
LpnPI CCDG 6 cut(s) 256, 470, 474, 513, 526, 540
Lsp1109I GCAGC 1 cut(s) 496
MaeI CTAG 3 cut(s) 134, 545, 586
MaeII ACGT 1 cut(s) 296
MaeIII GTNAC 1 cut(s) 5
MalI GATC 3 cut(s) 183, 202, 222
MbiI CCGCTC 1 cut(s) 442
MboI GATC 3 cut(s) 181, 200, 220
MboII GAAGA 3 cut(s) 145, 292, 368
MhlI GDGCHC 1 cut(s) 470
MluCI AATT 2 cut(s) 508, 574
MlyI GAGTC 1 cut(s) 484
MnlI CCTC 4 cut(s) 113, 231, 340, 496
MroNI GCCGGC 1 cut(s) 460
MseI TTAA 1 cut(s) 573
MslI CAYNNNNRTG 1 cut(s) 410
MspA1I CMGCKG 1 cut(s) 487
MspI CCGG 2 cut(s) 461, 500
MwoI GCNNNNNNNGC 2 cut(s) 49, 64
NaeI GCCGGC 1 cut(s) 462
NdeII GATC 3 cut(s) 181, 200, 220
NgoMIV GCCGGC 1 cut(s) 460
NlaIII CATG 5 cut(s) 91, 142, 398, 415, 474
NspI RCATGY 1 cut(s) 415
NspV TTCGAA 1 cut(s) 170
PciI ACATGT 1 cut(s) 411
PdiI GCCGGC 1 cut(s) 462
PkrI GCNGC 2 cut(s) 124, 486
PleI GAGTC 1 cut(s) 483
PpsI GAGTC 1 cut(s) 483
PscI ACATGT 1 cut(s) 411
Psp124BI GAGCTC 1 cut(s) 470
PvuII CAGCTG 1 cut(s) 487
RsaI GTAC 2 cut(s) 143, 275
RsaNI GTAC 2 cut(s) 142, 274
RseI CAYNNNNRTG 1 cut(s) 410
SacI GAGCTC 1 cut(s) 470
SaqAI TTAA 1 cut(s) 573
SatI GCNGC 2 cut(s) 123, 485
Sau3AI GATC 3 cut(s) 181, 200, 220
SchI GAGTC 1 cut(s) 484
SduI GDGCHC 1 cut(s) 470
SfuI TTCGAA 1 cut(s) 170
SmiMI CAYNNNNRTG 1 cut(s) 410
SmlI CTYRAG 1 cut(s) 226
SmoI CTYRAG 1 cut(s) 226
Sse9I AATT 2 cut(s) 508, 574
SsiI CCGC 7 cut(s) 56, 65, 123, 175, 191, 440, 482
SspMI CTAG 3 cut(s) 134, 545, 586
SstI GAGCTC 1 cut(s) 470
TaaI ACNGT 1 cut(s) 535
TaiI ACGT 1 cut(s) 299
TaqI TCGA 2 cut(s) 170, 318
TasI AATT 2 cut(s) 508, 574
TatI WGTACW 1 cut(s) 141
TauI GCSGC 1 cut(s) 125
Tru1I TTAA 1 cut(s) 573
Tru9I TTAA 1 cut(s) 573
TseI GCWGC 1 cut(s) 484
TspDTI ATGAA 2 cut(s) 321, 383
TspGWI ACGGA 2 cut(s) 366, 390
XapI RAATTY 1 cut(s) 508
XceI RCATGY 1 cut(s) 415
XspI CTAG 3 cut(s) 134, 545, 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.