MD07G1195700.v1.1

finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004301
Physical Location & Seq
Forward (+)
27451463 .. 27451882
420 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1195700.v1.1.491

Sequence Viewer

Length: 420 bp
ATGGCTTTCGAGGACATAGATTCTGAGTTGGCTTTTGTAATTGTTTCCGGTATATATGTGCTGGTGATAGGCATATGTTTGTGTTGCAGTGGCTCACCGCGCAGAAACGTCCCCCAAGAAGAAGCAACACCCCGTCGTTTGGGCCCCGAAATGCAAGAGACTTCAGCAGCAAGCAACATGGAAACACAAATCAATTGTGTCCAGACGAAGTACCAAAAGGGTGTGGGTCTGGTTTTGGTGGGAGACGGTACGTGTCCCGTGTGCCTAGACGAATTCGAAGAAGGTGAATATCTTCGTACATTGCCGGAGTGCAGCCACTCGTTTCATGTTCCATGCATAGACATGTGGCTCTATTCACAACCGAGTTGTCCGGTTTGCCGTGCCAATGCCAAGCTGGTTACAGTGGCCGTTAAGCAATAG

Protein Analysis

140

Amino Acids

15.26

Weight (kDa)

4.72

Isoelectric Point (pI)

55.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-ANAPC11 PF12861 82 - 131 2.9e-07 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-rbx1 PF12678 83 - 127 2.2e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 84 - 127 1.3e-14 Ring finger domain
zf-RING_11 PF17123 85 - 113 1.3e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 85 - 126 1.1e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 85 - 126 1.2e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 85 - 124 1.4e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 100
AciI CCGC 1 cut(s) 98
AcoI YGGCCR 1 cut(s) 405
AcsI RAATTY 1 cut(s) 272
AcuI CTGAAG 1 cut(s) 147
AfaI GTAC 3 cut(s) 212, 250, 298
AfiI CCNNNNNNNGG 1 cut(s) 139
AflIII ACRYGT 2 cut(s) 251, 342
AluBI AGCT 1 cut(s) 394
AluI AGCT 1 cut(s) 394
Alw26I GTCTC 2 cut(s) 152, 237
AoxI GGCC 2 cut(s) 142, 405
ApaI GGGCCC 1 cut(s) 146
ApeKI GCWGC 2 cut(s) 167, 312
ApoI RAATTY 1 cut(s) 272
Asp700I GAANNNNTTC 1 cut(s) 291
AspLEI GCGC 1 cut(s) 102
AspS9I GGNCC 2 cut(s) 142, 143
AsuHPI GGTGA 3 cut(s) 76, 87, 296
AsuII TTCGAA 1 cut(s) 276
BaeGI GKGCMC 1 cut(s) 146
BanII GRGCYC 1 cut(s) 146
BbvI GCAGC 2 cut(s) 179, 324
BceAI ACGGC 2 cut(s) 363, 392
BcoDI GTCTC 2 cut(s) 152, 237
BfaI CTAG 1 cut(s) 266
BisI GCNGC 2 cut(s) 168, 313
BlsI GCNGC 2 cut(s) 169, 314
BmgT120I GGNCC 2 cut(s) 142, 143
BmiI GGNNCC 2 cut(s) 144, 145
Bpu14I TTCGAA 1 cut(s) 276
BsaAI YACGTR 1 cut(s) 252
BsaWI WCCGGW 2 cut(s) 47, 370
Bsc4I CCNNNNNNNGG 1 cut(s) 139
Bse3DI GCAATG 1 cut(s) 299
BseLI CCNNNNNNNGG 1 cut(s) 139
BseMI GCAATG 1 cut(s) 299
BseMII CTCAG 1 cut(s) 15
BseSI GKGCMC 1 cut(s) 146
BseXI GCAGC 2 cut(s) 179, 324
BsgI GTGCAG 1 cut(s) 331
Bsh1236I CGCG 1 cut(s) 100
BshFI GGCC 2 cut(s) 144, 407
BsiSI CCGG 3 cut(s) 48, 305, 371
BslFI GGGAC 2 cut(s) 95, 240
BslI CCNNNNNNNGG 1 cut(s) 139
BsmAI GTCTC 2 cut(s) 152, 237
BsmBI CGTCTC 1 cut(s) 237
BsmFI GGGAC 2 cut(s) 95, 240
BsnI GGCC 2 cut(s) 144, 407
Bsp119I TTCGAA 1 cut(s) 276
Bsp120I GGGCCC 1 cut(s) 142
Bsp1286I GDGCHC 1 cut(s) 146
BspACI CCGC 1 cut(s) 98
BspANI GGCC 2 cut(s) 144, 407
BspCNI CTCAG 1 cut(s) 16
BspFNI CGCG 1 cut(s) 100
BspLI GGNNCC 2 cut(s) 144, 145
BspT104I TTCGAA 1 cut(s) 276
BsrDI GCAATG 1 cut(s) 299
Bst4CI ACNGT 2 cut(s) 248, 403
BstBAI YACGTR 1 cut(s) 252
BstBI TTCGAA 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 172
BstDEI CTNAG 1 cut(s) 24
BstFNI CGCG 1 cut(s) 100
BstHHI GCGC 1 cut(s) 102
BstMAI GTCTC 2 cut(s) 152, 237
BstMWI GCNNNNNNNGC 1 cut(s) 99
BstNSI RCATGY 1 cut(s) 346
BstSLI GKGCMC 1 cut(s) 146
BstUI CGCG 1 cut(s) 100
BstV1I GCAGC 2 cut(s) 179, 324
BsuRI GGCC 2 cut(s) 144, 407
BtsI GCAGTG 1 cut(s) 94
BtsIMutI CAGTG 2 cut(s) 94, 408
Cac8I GCNNGC 1 cut(s) 172
CfoI GCGC 1 cut(s) 102
Cfr13I GGNCC 2 cut(s) 142, 143
Csp6I GTAC 3 cut(s) 211, 249, 297
CviAII CATG 4 cut(s) 178, 326, 333, 343
CviJI RGCY 8 cut(s) 5, 32, 93, 144, 315, 349, 394, 407
CviKI_1 RGCY 8 cut(s) 5, 32, 93, 144, 315, 349, 394, 407
CviQI GTAC 3 cut(s) 211, 249, 297
DdeI CTNAG 1 cut(s) 24
EaeI YGGCCR 1 cut(s) 405
Eco24I GRGCYC 1 cut(s) 146
Eco57I CTGAAG 1 cut(s) 147
EcoO109I RGGNCCY 1 cut(s) 143
EcoRI GAATTC 1 cut(s) 272
EcoT22I ATGCAT 1 cut(s) 338
EcoT38I GRGCYC 1 cut(s) 146
Esp3I CGTCTC 1 cut(s) 237
FaeI CATG 4 cut(s) 181, 329, 336, 346
FaqI GGGAC 2 cut(s) 95, 240
FatI CATG 4 cut(s) 177, 325, 332, 342
FauNDI CATATG 1 cut(s) 74
Fnu4HI GCNGC 2 cut(s) 168, 313
FriOI GRGCYC 1 cut(s) 146
Fsp4HI GCNGC 2 cut(s) 168, 313
FspBI CTAG 1 cut(s) 266
GlaI GCGC 1 cut(s) 101
GluI GCNGC 2 cut(s) 168, 313
HaeIII GGCC 2 cut(s) 144, 407
HapII CCGG 3 cut(s) 48, 305, 371
HhaI GCGC 1 cut(s) 102
Hin1II CATG 4 cut(s) 181, 329, 336, 346
Hin6I GCGC 1 cut(s) 100
HinP1I GCGC 1 cut(s) 100
HinfI GANTC 1 cut(s) 20
HpaII CCGG 3 cut(s) 48, 305, 371
HphI GGTGA 3 cut(s) 76, 87, 296
Hpy188I TCNGA 1 cut(s) 25
Hpy188III TCNNGA 1 cut(s) 202
Hpy99I CGWCG 1 cut(s) 138
HpyAV CCTTC 1 cut(s) 275
HpyCH4III ACNGT 2 cut(s) 248, 403
HpyCH4IV ACGT 2 cut(s) 108, 251
HpyCH4V TGCA 4 cut(s) 87, 154, 312, 336
HpyF10VI GCNNNNNNNGC 1 cut(s) 99
HpyF3I CTNAG 1 cut(s) 24
HpySE526I ACGT 2 cut(s) 108, 251
Hsp92II CATG 4 cut(s) 181, 329, 336, 346
HspAI GCGC 1 cut(s) 100
LpnPI CCDG 7 cut(s) 47, 61, 215, 215, 318, 380, 384
Lsp1109I GCAGC 2 cut(s) 179, 324
MaeI CTAG 1 cut(s) 266
MaeII ACGT 2 cut(s) 108, 251
MaeIII GTNAC 1 cut(s) 397
MboII GAAGA 3 cut(s) 131, 284, 290
MfeI CAATTG 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 146
MluCI AATT 3 cut(s) 39, 193, 272
MnlI CCTC 1 cut(s) 4
Mph1103I ATGCAT 1 cut(s) 338
MroXI GAANNNNTTC 1 cut(s) 291
MseI TTAA 1 cut(s) 411
MslI CAYNNNNRTG 1 cut(s) 341
MspI CCGG 3 cut(s) 48, 305, 371
MunI CAATTG 1 cut(s) 193
MvnI CGCG 1 cut(s) 100
MwoI GCNNNNNNNGC 1 cut(s) 99
NdeI CATATG 1 cut(s) 74
NlaIII CATG 4 cut(s) 181, 329, 336, 346
NlaIV GGNNCC 2 cut(s) 144, 145
NsiI ATGCAT 1 cut(s) 338
NspI RCATGY 1 cut(s) 346
NspV TTCGAA 1 cut(s) 276
PciI ACATGT 1 cut(s) 342
PdmI GAANNNNTTC 1 cut(s) 291
PfeI GAWTC 1 cut(s) 20
PkrI GCNGC 2 cut(s) 169, 314
Ppu21I YACGTR 1 cut(s) 252
PscI ACATGT 1 cut(s) 342
PspN4I GGNNCC 2 cut(s) 144, 145
PspOMI GGGCCC 1 cut(s) 142
PspPI GGNCC 2 cut(s) 142, 143
RsaI GTAC 3 cut(s) 212, 250, 298
RsaNI GTAC 3 cut(s) 211, 249, 297
RseI CAYNNNNRTG 1 cut(s) 341
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 2 cut(s) 168, 313
Sau96I GGNCC 2 cut(s) 142, 143
SduI GDGCHC 1 cut(s) 146
SetI ASST 4 cut(s) 111, 254, 286, 396
SfuI TTCGAA 1 cut(s) 276
SmiMI CAYNNNNRTG 1 cut(s) 341
Sse9I AATT 3 cut(s) 39, 193, 272
SsiI CCGC 1 cut(s) 98
SspMI CTAG 1 cut(s) 266
TaaI ACNGT 2 cut(s) 248, 403
TaiI ACGT 2 cut(s) 111, 254
TaqI TCGA 2 cut(s) 9, 276
TasI AATT 3 cut(s) 39, 193, 272
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 2 cut(s) 94, 408
TseI GCWGC 2 cut(s) 167, 312
TspDTI ATGAA 1 cut(s) 314
TspRI CASTG 2 cut(s) 94, 408
XapI RAATTY 1 cut(s) 272
XceI RCATGY 1 cut(s) 346
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XmnI GAANNNNTTC 1 cut(s) 291
XspI CTAG 1 cut(s) 266
Zsp2I ATGCAT 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.