pycom09g10280

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0004301
Physical Location & Seq
Forward (+)
8399144 .. 8399671
528 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g10280.1

Sequence Viewer

Length: 528 bp
ATGTTAGTCGTATCAGGCATCGTGGTATTTGGTGTGCTCGTCATTTCAGTCGTAATCGGATGGTATGTATATCGTATGCGAATACCATTGTCTCCTCCGACTAGCAACGAGCGTGTCCAGAAGATCGAATGCACGGTTGATAAGAGGTTGAAGCCGGATTTAATCACATTCAGTTATAAGAAAGAGAGTGATGATGGTGATCCTGATCAAAACGAAGATGACGAAGAAGCTTTATATCGTAAAACTAGTACTACCATCGAATGTGTAGTGTGCTTGAGAGGTTTGGAAGATGAGGAAACTGTGAGGCGGCTACCAAAATGCAAGCACATCTTCCACGCACCTTGTATTGACATGTGGCTTGATTCTCATTCCGGCTGCCCTATCTGCCGTACTCTGATTGACCAACTGCGTTCCGGCAATAACCAGCTGTCATTTACACCTCACGAGGATCAAGAGAATTCCATGGAAGTCACGCCCACAGAAGTAGATAATATCAGAACCTCAATGTTATCTATCCCAATCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

176

Amino Acids

19.94

Weight (kDa)

4.91

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 87 - 130 1.3e-13 Ring finger domain
zf-RING_11 PF17123 87 - 116 2e-09 RING-like zinc finger
zf-rbx1 PF12678 87 - 130 1.2e-08 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 87 - 129 2e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 88 - 129 7.5e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AciI CCGC 1 cut(s) 307
AclWI GGATC 2 cut(s) 194, 456
AcsI RAATTY 1 cut(s) 457
AfaI GTAC 2 cut(s) 250, 391
AflIII ACRYGT 1 cut(s) 351
AgsI TTSAA 1 cut(s) 151
AhlI ACTAGT 1 cut(s) 245
AluBI AGCT 2 cut(s) 230, 427
AluI AGCT 2 cut(s) 230, 427
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 96
AlwI GGATC 2 cut(s) 194, 456
ApeKI GCWGC 1 cut(s) 375
ApoI RAATTY 1 cut(s) 457
AsuHPI GGTGA 1 cut(s) 209
BauI CACGAG 1 cut(s) 443
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 1 cut(s) 362
BccI CCATC 3 cut(s) 54, 188, 263
BceAI ACGGC 1 cut(s) 372
BclI TGATCA 1 cut(s) 205
BcoDI GTCTC 1 cut(s) 96
BcuI ACTAGT 1 cut(s) 245
BfaI CTAG 2 cut(s) 102, 246
BisI GCNGC 2 cut(s) 308, 376
BlsI GCNGC 2 cut(s) 309, 377
BmcAI AGTACT 1 cut(s) 250
BmsI GCATC 1 cut(s) 27
BpuEI CTTGAG 1 cut(s) 295
BsaBI GATNNNNATC 2 cut(s) 198, 204
BsaJI CCNNGG 1 cut(s) 462
Bse8I GATNNNNATC 2 cut(s) 198, 204
BseDI CCNNGG 1 cut(s) 462
BseGI GGATG 1 cut(s) 65
BseJI GATNNNNATC 2 cut(s) 198, 204
BseRI GAGGAG 1 cut(s) 84
BseXI GCAGC 1 cut(s) 362
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 3 cut(s) 155, 372, 414
BsmAI GTCTC 1 cut(s) 96
BsmI GAATGC 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 4 cut(s) 123, 199, 205, 448
Bsp19I CCATGG 1 cut(s) 462
BspACI CCGC 1 cut(s) 307
BspPI GGATC 2 cut(s) 194, 456
BssECI CCNNGG 1 cut(s) 462
BssMI GATC 4 cut(s) 123, 199, 205, 448
BssSI CACGAG 1 cut(s) 443
BssT1I CCWWGG 1 cut(s) 462
Bst2BI CACGAG 1 cut(s) 443
Bst4CI ACNGT 2 cut(s) 136, 301
BstC8I GCNNGC 1 cut(s) 323
BstDSI CCRYGG 1 cut(s) 462
BstF5I GGATG 1 cut(s) 65
BstKTI GATC 4 cut(s) 126, 202, 208, 451
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 4 cut(s) 123, 199, 205, 448
BstMWI GCNNNNNNNGC 1 cut(s) 384
BstNSI RCATGY 1 cut(s) 355
BstV1I GCAGC 1 cut(s) 362
BtgI CCRYGG 1 cut(s) 462
BtsCI GGATG 1 cut(s) 65
Cac8I GCNNGC 1 cut(s) 323
Csp6I GTAC 2 cut(s) 249, 390
CspCI CAANNNNNGTGG 2 cut(s) 323, 358
CviAII CATG 2 cut(s) 352, 463
CviJI RGCY 6 cut(s) 154, 230, 310, 358, 375, 427
CviKI_1 RGCY 6 cut(s) 154, 230, 310, 358, 375, 427
CviQI GTAC 2 cut(s) 249, 390
DpnI GATC 4 cut(s) 125, 201, 207, 450
DpnII GATC 4 cut(s) 123, 199, 205, 448
Eco130I CCWWGG 1 cut(s) 462
EcoRI GAATTC 1 cut(s) 457
EcoT14I CCWWGG 1 cut(s) 462
ErhI CCWWGG 1 cut(s) 462
FaeI CATG 2 cut(s) 355, 466
FaiI YATR 7 cut(s) 66, 70, 77, 177, 235, 353, 464
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FatI CATG 2 cut(s) 351, 462
FbaI TGATCA 1 cut(s) 205
Fnu4HI GCNGC 2 cut(s) 308, 376
FokI GGATG 1 cut(s) 72
Fsp4HI GCNGC 2 cut(s) 308, 376
FspBI CTAG 2 cut(s) 102, 246
GluI GCNGC 2 cut(s) 308, 376
HapII CCGG 3 cut(s) 155, 372, 414
Hin1II CATG 2 cut(s) 355, 466
HindIII AAGCTT 1 cut(s) 228
HinfI GANTC 1 cut(s) 362
HpaII CCGG 3 cut(s) 155, 372, 414
HphI GGTGA 1 cut(s) 209
Hpy188I TCNGA 4 cut(s) 59, 99, 396, 497
Hpy188III TCNNGA 4 cut(s) 118, 203, 443, 452
HpyCH4III ACNGT 2 cut(s) 136, 301
HpyCH4V TGCA 2 cut(s) 132, 321
HpyF10VI GCNNNNNNNGC 1 cut(s) 384
Hsp92II CATG 2 cut(s) 355, 466
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 4 cut(s) 123, 199, 205, 448
LpnPI CCDG 6 cut(s) 131, 168, 216, 385, 427, 437
Lsp1109I GCAGC 1 cut(s) 362
LweI GCATC 1 cut(s) 27
MaeI CTAG 2 cut(s) 102, 246
MaeIII GTNAC 1 cut(s) 469
MalI GATC 4 cut(s) 125, 201, 207, 450
MboI GATC 4 cut(s) 123, 199, 205, 448
MboII GAAGA 5 cut(s) 133, 227, 236, 299, 322
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 1 cut(s) 457
MmeI TCCRAC 1 cut(s) 122
MnlI CCTC 8 cut(s) 105, 138, 272, 286, 297, 439, 450, 511
MseI TTAA 1 cut(s) 161
MspA1I CMGCKG 1 cut(s) 427
MspI CCGG 3 cut(s) 155, 372, 414
Mva1269I GAATGC 1 cut(s) 134
MwoI GCNNNNNNNGC 1 cut(s) 384
NcoI CCATGG 1 cut(s) 462
NdeII GATC 4 cut(s) 123, 199, 205, 448
NlaIII CATG 2 cut(s) 355, 466
NmuCI GTSAC 1 cut(s) 469
NspI RCATGY 1 cut(s) 355
PciI ACATGT 1 cut(s) 351
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 1 cut(s) 134
PfeI GAWTC 1 cut(s) 362
PkrI GCNGC 2 cut(s) 309, 377
PscI ACATGT 1 cut(s) 351
PsiI TTATAA 1 cut(s) 177
PvuII CAGCTG 1 cut(s) 427
RsaI GTAC 2 cut(s) 250, 391
RsaNI GTAC 2 cut(s) 249, 390
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 2 cut(s) 308, 376
Sau3AI GATC 4 cut(s) 123, 199, 205, 448
ScaI AGTACT 1 cut(s) 250
SduI GDGCHC 1 cut(s) 39
SetI ASST 7 cut(s) 149, 232, 283, 343, 429, 442, 503
SfaNI GCATC 1 cut(s) 27
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
SpeI ACTAGT 1 cut(s) 245
Sse9I AATT 1 cut(s) 457
SsiI CCGC 1 cut(s) 307
SspMI CTAG 2 cut(s) 102, 246
StyI CCWWGG 1 cut(s) 462
TaaI ACNGT 2 cut(s) 136, 301
TaqI TCGA 2 cut(s) 126, 258
TasI AATT 1 cut(s) 457
TatI WGTACW 1 cut(s) 248
TauI GCSGC 1 cut(s) 310
TfiI GAWTC 1 cut(s) 362
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TseFI GTSAC 1 cut(s) 469
TseI GCWGC 1 cut(s) 375
Tsp45I GTSAC 1 cut(s) 469
XapI RAATTY 1 cut(s) 457
XceI RCATGY 1 cut(s) 355
XspI CTAG 2 cut(s) 102, 246
ZrmI AGTACT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.