Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0004301

Physical Location & Seq

Genomic Coordinates Reverse (-)

59283098 .. 59283601

Gene Structure 504 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G036140.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 504 bp

ATGTCAATATCATTGCTTTTTTCTGGCATTATGATCTTAGGGGTGATACTCATTTGCTTTGTAATTTACATTGTATTTACAGTAGCATGGTATTTATATGGTAGGCAAATACTATCTGCTCCAACATTAAGCCAGCAGGAGGACCCAGCCAAGGAGAAGTACTTTAGTTACCAGAAACTTAAGCCGGATGTGATCACATTCAACTACAAGAAAGAATTGAGTGATGATCCAAATCACGAAGAAGAGAATTCTGATCAAAATGAATGCGTGGTGTGCTTAAGAGGTTTACAGAATGAGGAATTTGTGAGGCAACTGCCAAAATGCAAGCACACATTCCACGCGCCTTGTATAGACATGTGGCTCTACTCTCACTCTGATTGCCCCATCTGTCGGATGGTGATTGATCAGTTGCCTGATGAAGGTGCATTTACACCAGGCCGGAAGTCGGAGGTCTCTGTAGATATCAGCCCATCACTGACCTTAACAACCATCTCAATAATCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

167

Amino Acids

19.1

Weight (kDa)

5.01

Isoelectric Point (pI)

42.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	87 - 131	7.6e-13	Ring finger domain
zf-rbx1	PF12678	87 - 131	2.2e-08	RING-H2 zinc finger domain
zf-RING_11	PF17123	88 - 117	7.5e-09	RING-like zinc finger
zf-C3HC4_2	PF13923	88 - 130	1.1e-07	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05280
fragaria_vesca	FvH4_1g08250
malus_domestica	MD01G1126200.v1.1 MD02G1086800.v1.1 MD07G1195700.v1.1 MD07G1195800.v1.1 MD07G1196000.v1.1 MD07G1196100.v1.1 MD15G1214000.v1.1
prunus_persica	Prupe.2G233100_v2.0.a1 Prupe.3G043300_v2.0.a1 Prupe.7G203000_v2.0.a1
pyrus_communis	pycom01g15190 pycom09g10280 pycom15g19020
rosa_chinensis	RchiOBHm_Chr2g0094301 RchiOBHm_Chr2g0144911
rosa_laevigata	RLG00000016475 RLG00000020118
rosa_multiflora	Rmu_sc0002354.1_g000011 Rmu_sc0003819.1_g000026
rosa_roxburghii	Rroxscaffold_2G00147280
rosa_rugosa	Rorug02G0042800
rosa_samantha	Rh2AG090600 Rh2AG442000 Rh2BG090500 Rh2BG452600 Rh2CG093000 Rh2CG429300 Rh2DG089500 Rh2DG462500
rosa_wichuraiana	Rw2G036140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	341
AclWI	GGATC	1 cut(s)	221
AcsI	RAATTY	2 cut(s)	247, 299
AfaI	GTAC	1 cut(s)	161
AfiI	CCNNNNNNNGG	5 cut(s)	139, 151, 390, 419, 445
AflII	CTTAAG	2 cut(s)	179, 277
AflIII	ACRYGT	1 cut(s)	354
AgsI	TTSAA	1 cut(s)	202
AjnI	CCWGG	1 cut(s)	433
Alw26I	GTCTC	1 cut(s)	457
AlwI	GGATC	1 cut(s)	221
AoxI	GGCC	1 cut(s)	436
ApoI	RAATTY	2 cut(s)	247, 299
AspLEI	GCGC	1 cut(s)	343
AspS9I	GGNCC	1 cut(s)	142
AsuHPI	GGTGA	2 cut(s)	55, 409
AvaII	GGWCC	1 cut(s)	142
BccI	CCATC	4 cut(s)	388, 392, 478, 497
BciT130I	CCWGG	1 cut(s)	435
BclI	TGATCA	3 cut(s)	192, 253, 403
BcoDI	GTCTC	1 cut(s)	457
BfaI	CTAG	1 cut(s)	502
BfmI	CTRYAG	1 cut(s)	456
BfrI	CTTAAG	2 cut(s)	179, 277
BmcAI	AGTACT	1 cut(s)	161
Bme1390I	CCNGG	1 cut(s)	435
Bme18I	GGWCC	1 cut(s)	142
BmgT120I	GGNCC	1 cut(s)	142
BmiI	GGNNCC	1 cut(s)	144
BmrFI	CCNGG	1 cut(s)	435
BsaBI	GATNNNNATC	1 cut(s)	231
BsaI	GGTCTC	1 cut(s)	457
BsaJI	CCNNGG	1 cut(s)	150
Bsc4I	CCNNNNNNNGG	5 cut(s)	139, 151, 390, 419, 445
Bse3DI	GCAATG	1 cut(s)	11
Bse8I	GATNNNNATC	1 cut(s)	231
BseBI	CCWGG	1 cut(s)	435
BseDI	CCNNGG	1 cut(s)	150
BseGI	GGATG	2 cut(s)	193, 399
BseJI	GATNNNNATC	1 cut(s)	231
BseLI	CCNNNNNNNGG	5 cut(s)	139, 151, 390, 419, 445
BseMI	GCAATG	1 cut(s)	11
BseYI	CCCAGC	1 cut(s)	145
Bsh1236I	CGCG	1 cut(s)	341
BshFI	GGCC	1 cut(s)	438
BsiSI	CCGG	2 cut(s)	185, 439
BslI	CCNNNNNNNGG	5 cut(s)	139, 151, 390, 419, 445
BsmAI	GTCTC	1 cut(s)	457
BsmI	GAATGC	1 cut(s)	269
BsnI	GGCC	1 cut(s)	438
Bso31I	GGTCTC	1 cut(s)	457
Bsp143I	GATC	5 cut(s)	33, 192, 226, 253, 403
BspANI	GGCC	1 cut(s)	438
BspFNI	CGCG	1 cut(s)	341
BspLI	GGNNCC	1 cut(s)	144
BspPI	GGATC	1 cut(s)	221
BspTI	CTTAAG	2 cut(s)	179, 277
BspTNI	GGTCTC	1 cut(s)	457
BsrDI	GCAATG	1 cut(s)	11
BssECI	CCNNGG	1 cut(s)	150
BssMI	GATC	5 cut(s)	33, 192, 226, 253, 403
BssT1I	CCWWGG	1 cut(s)	150
Bst2UI	CCWGG	1 cut(s)	435
Bst4CI	ACNGT	1 cut(s)	82
Bst6I	CTCTTC	1 cut(s)	237
BstAFI	CTTAAG	2 cut(s)	179, 277
BstC8I	GCNNGC	2 cut(s)	134, 326
BstDEI	CTNAG	1 cut(s)	37
BstENI	CCTNNNNNAGG	1 cut(s)	417
BstF5I	GGATG	2 cut(s)	193, 399
BstFNI	CGCG	1 cut(s)	341
BstHHI	GCGC	1 cut(s)	343
BstKTI	GATC	5 cut(s)	36, 195, 229, 256, 406
BstMAI	GTCTC	1 cut(s)	457
BstMBI	GATC	5 cut(s)	33, 192, 226, 253, 403
BstMWI	GCNNNNNNNGC	1 cut(s)	273
BstNI	CCWGG	1 cut(s)	435
BstNSI	RCATGY	1 cut(s)	358
BstSCI	CCNGG	1 cut(s)	433
BstSFI	CTRYAG	1 cut(s)	456
BstUI	CGCG	1 cut(s)	341
BsuRI	GGCC	1 cut(s)	438
BtsCI	GGATG	2 cut(s)	193, 399
BtsIMutI	CAGTG	1 cut(s)	473
Cac8I	GCNNGC	2 cut(s)	134, 326
CfoI	GCGC	1 cut(s)	343
Cfr13I	GGNCC	1 cut(s)	142
Csp6I	GTAC	1 cut(s)	160
CspCI	CAANNNNNGTGG	2 cut(s)	326, 361
CviAII	CATG	2 cut(s)	87, 355
CviJI	RGCY	6 cut(s)	132, 149, 184, 361, 438, 468
CviKI_1	RGCY	6 cut(s)	132, 149, 184, 361, 438, 468
CviQI	GTAC	1 cut(s)	160
DdeI	CTNAG	1 cut(s)	37
DpnI	GATC	5 cut(s)	35, 194, 228, 255, 405
DpnII	GATC	5 cut(s)	33, 192, 226, 253, 403
Eam1104I	CTCTTC	1 cut(s)	237
EarI	CTCTTC	1 cut(s)	237
Eco130I	CCWWGG	1 cut(s)	150
Eco31I	GGTCTC	1 cut(s)	457
Eco32I	GATATC	1 cut(s)	463
Eco47I	GGWCC	1 cut(s)	142
EcoNI	CCTNNNNNAGG	1 cut(s)	417
EcoO109I	RGGNCCY	1 cut(s)	142
EcoRI	GAATTC	1 cut(s)	247
EcoRII	CCWGG	1 cut(s)	433
EcoRV	GATATC	1 cut(s)	463
EcoT14I	CCWWGG	1 cut(s)	150
ErhI	CCWWGG	1 cut(s)	150
FaeI	CATG	2 cut(s)	90, 358
FaiI	YATR	6 cut(s)	32, 88, 97, 99, 350, 356
FatI	CATG	2 cut(s)	86, 354
FbaI	TGATCA	3 cut(s)	192, 253, 403
FokI	GGATG	2 cut(s)	200, 406
FspBI	CTAG	1 cut(s)	502
GlaI	GCGC	1 cut(s)	342
GsaI	CCCAGC	1 cut(s)	149
HaeIII	GGCC	1 cut(s)	438
HapII	CCGG	2 cut(s)	185, 439
HhaI	GCGC	1 cut(s)	343
Hin1II	CATG	2 cut(s)	90, 358
Hin6I	GCGC	1 cut(s)	341
HinP1I	GCGC	1 cut(s)	341
HpaII	CCGG	2 cut(s)	185, 439
HphI	GGTGA	2 cut(s)	55, 409
Hpy166II	GTNNAC	1 cut(s)	287
Hpy188I	TCNGA	4 cut(s)	253, 376, 393, 448
Hpy188III	TCNNGA	1 cut(s)	236
Hpy8I	GTNNAC	1 cut(s)	287
HpyAV	CCTTC	1 cut(s)	413
HpyCH4III	ACNGT	1 cut(s)	82
HpyCH4V	TGCA	2 cut(s)	324, 425
HpyF10VI	GCNNNNNNNGC	1 cut(s)	273
HpyF3I	CTNAG	1 cut(s)	37
Hsp92II	CATG	2 cut(s)	90, 358
HspAI	GCGC	1 cut(s)	341
Ksp22I	TGATCA	3 cut(s)	192, 253, 403
Kzo9I	GATC	5 cut(s)	33, 192, 226, 253, 403
LmnI	GCTCC	1 cut(s)	124
MaeI	CTAG	1 cut(s)	502
MaeIII	GTNAC	1 cut(s)	167
MalI	GATC	5 cut(s)	35, 194, 228, 255, 405
MboI	GATC	5 cut(s)	33, 192, 226, 253, 403
MboII	GAAGA	2 cut(s)	251, 254
MluCI	AATT	4 cut(s)	63, 215, 247, 299
MmeI	TCCRAC	3 cut(s)	146, 371, 426
MnlI	CCTC	5 cut(s)	133, 275, 289, 300, 442
MseI	TTAA	4 cut(s)	128, 180, 278, 482
MspCI	CTTAAG	2 cut(s)	179, 277
MspI	CCGG	2 cut(s)	185, 439
MspR9I	CCNGG	1 cut(s)	435
Mva1269I	GAATGC	1 cut(s)	269
MvaI	CCWGG	1 cut(s)	435
MvnI	CGCG	1 cut(s)	341
MwoI	GCNNNNNNNGC	1 cut(s)	273
NdeII	GATC	5 cut(s)	33, 192, 226, 253, 403
NlaIII	CATG	2 cut(s)	90, 358
NlaIV	GGNNCC	1 cut(s)	144
NspI	RCATGY	1 cut(s)	358
PciI	ACATGT	1 cut(s)	354
PctI	GAATGC	1 cut(s)	269
PpuMI	RGGWCCY	1 cut(s)	142
PscI	ACATGT	1 cut(s)	354
Psp5II	RGGWCCY	1 cut(s)	142
Psp6I	CCWGG	1 cut(s)	433
PspFI	CCCAGC	1 cut(s)	145
PspGI	CCWGG	1 cut(s)	433
PspN4I	GGNNCC	1 cut(s)	144
PspPI	GGNCC	1 cut(s)	142
PspPPI	RGGWCCY	1 cut(s)	142
RsaI	GTAC	1 cut(s)	161
RsaNI	GTAC	1 cut(s)	160
SaqAI	TTAA	4 cut(s)	128, 180, 278, 482
Sau3AI	GATC	5 cut(s)	33, 192, 226, 253, 403
Sau96I	GGNCC	1 cut(s)	142
ScaI	AGTACT	1 cut(s)	161
ScrFI	CCNGG	1 cut(s)	435
SetI	ASST	4 cut(s)	286, 424, 453, 482
SfcI	CTRYAG	1 cut(s)	456
SinI	GGWCC	1 cut(s)	142
SmlI	CTYRAG	2 cut(s)	179, 277
SmoI	CTYRAG	2 cut(s)	179, 277
Sse9I	AATT	4 cut(s)	63, 215, 247, 299
SspMI	CTAG	1 cut(s)	502
StyD4I	CCNGG	1 cut(s)	433
StyI	CCWWGG	1 cut(s)	150
TaaI	ACNGT	1 cut(s)	82
TasI	AATT	4 cut(s)	63, 215, 247, 299
TatI	WGTACW	1 cut(s)	159
Tru1I	TTAA	4 cut(s)	128, 180, 278, 482
Tru9I	TTAA	4 cut(s)	128, 180, 278, 482
TscAI	CASTG	1 cut(s)	480
TspDTI	ATGAA	2 cut(s)	276, 432
TspRI	CASTG	1 cut(s)	480
Vha464I	CTTAAG	2 cut(s)	179, 277
VpaK11BI	GGWCC	1 cut(s)	142
XagI	CCTNNNNNAGG	1 cut(s)	417
XapI	RAATTY	2 cut(s)	247, 299
XceI	RCATGY	1 cut(s)	358
XcmI	CCANNNNNNNNNTGG	1 cut(s)	391
XspI	CTAG	1 cut(s)	502
ZrmI	AGTACT	1 cut(s)	161

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.