MD07G1195800.v1.1

Anaphase-promoting complex subunit 11 RING-H2 finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004301
Physical Location & Seq
Forward (+)
27455309 .. 27455680
372 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1195800.v1.1.491

Sequence Viewer

Length: 372 bp
ATGCCTTTCGAGGACACAATTTCTGGCGACTCCTCGGGTTTTCTAATTGTTATATCTGGGATTGTAATACTACTAGGCATATGTTTGTGTTGTGGCTCATGCTGCAGAAACATTCCCGAAGAAGAAGAAACACCAGCAAGCAACGTGGAAACCCAGATCAATTGGGTGCATAGGAAGTACCAGAAGGGTGTGGGTCTGGTTTTGGTCGGAGACGGTACGTGTGCCGTGTGCCTAGGCGAATATTTTCGTACGTTGCCGGACTGCAGCCACTCGTTTCATGTTCAATGCATTGACATGTGGCTCTATTCACAACCGAGTTGCCTGGTTTGCCGTGCCAATGCCACGCCGGTTACATTGGTCGCTAAGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

124

Amino Acids

13.4

Weight (kDa)

4.95

Isoelectric Point (pI)

45.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 73 - 111 1.7e-08 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 3 cut(s) 179, 217, 250
AflIII ACRYGT 2 cut(s) 218, 294
AgsI TTSAA 1 cut(s) 284
AjnI CCWGG 1 cut(s) 321
Alw26I GTCTC 1 cut(s) 204
Ama87I CYCGRG 1 cut(s) 34
ApeKI GCWGC 2 cut(s) 102, 264
Asp700I GAANNNNTTC 1 cut(s) 243
AspA2I CCTAGG 1 cut(s) 232
AvaI CYCGRG 1 cut(s) 34
AvrII CCTAGG 1 cut(s) 232
BbvI GCAGC 2 cut(s) 89, 276
BceAI ACGGC 2 cut(s) 209, 315
BciT130I CCWGG 1 cut(s) 323
BcoDI GTCTC 1 cut(s) 204
BfaI CTAG 2 cut(s) 74, 233
BfmI CTRYAG 2 cut(s) 103, 262
BisI GCNGC 2 cut(s) 103, 265
BlnI CCTAGG 1 cut(s) 232
BlpI GCTNAGC 1 cut(s) 363
BlsI GCNGC 2 cut(s) 104, 266
Bme1390I CCNGG 1 cut(s) 323
BmeT110I CYCGRG 1 cut(s) 34
BmrFI CCNGG 1 cut(s) 323
Bpu1102I GCTNAGC 1 cut(s) 363
BsaAI YACGTR 1 cut(s) 219
BsaJI CCNNGG 2 cut(s) 33, 232
Bse118I RCCGGY 1 cut(s) 346
BseBI CCWGG 1 cut(s) 323
BseDI CCNNGG 2 cut(s) 33, 232
BseRI GAGGAG 1 cut(s) 22
BseXI GCAGC 2 cut(s) 89, 276
BsiHKCI CYCGRG 1 cut(s) 34
BsiSI CCGG 2 cut(s) 257, 347
BsiWI CGTACG 1 cut(s) 248
BsmAI GTCTC 1 cut(s) 204
BsmBI CGTCTC 1 cut(s) 204
BsoBI CYCGRG 1 cut(s) 34
Bsp143I GATC 1 cut(s) 156
Bsp1720I GCTNAGC 1 cut(s) 363
BspMAI CTGCAG 2 cut(s) 107, 266
BsrFI RCCGGY 1 cut(s) 346
BssAI RCCGGY 1 cut(s) 346
BssECI CCNNGG 2 cut(s) 33, 232
BssMI GATC 1 cut(s) 156
BssT1I CCWWGG 1 cut(s) 232
Bst2UI CCWGG 1 cut(s) 323
Bst4CI ACNGT 1 cut(s) 215
BstBAI YACGTR 1 cut(s) 219
BstC8I GCNNGC 1 cut(s) 139
BstDEI CTNAG 1 cut(s) 363
BstKTI GATC 1 cut(s) 159
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 1 cut(s) 156
BstMWI GCNNNNNNNGC 2 cut(s) 102, 327
BstNI CCWGG 1 cut(s) 323
BstNSI RCATGY 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 321
BstSFI CTRYAG 2 cut(s) 103, 262
BstV1I GCAGC 2 cut(s) 89, 276
Cac8I GCNNGC 1 cut(s) 139
Cfr10I RCCGGY 1 cut(s) 346
Csp6I GTAC 3 cut(s) 178, 216, 249
CspCI CAANNNNNGTGG 2 cut(s) 126, 161
CviAII CATG 3 cut(s) 99, 278, 295
CviJI RGCY 3 cut(s) 96, 267, 301
CviKI_1 RGCY 3 cut(s) 96, 267, 301
CviQI GTAC 3 cut(s) 178, 216, 249
DdeI CTNAG 1 cut(s) 363
DpnI GATC 1 cut(s) 158
DpnII GATC 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 232
Eco88I CYCGRG 1 cut(s) 34
EcoRII CCWGG 1 cut(s) 321
EcoT14I CCWWGG 1 cut(s) 232
EcoT22I ATGCAT 1 cut(s) 290
ErhI CCWWGG 1 cut(s) 232
Esp3I CGTCTC 1 cut(s) 204
FaeI CATG 3 cut(s) 102, 281, 298
FaiI YATR 7 cut(s) 53, 80, 82, 100, 171, 279, 296
FatI CATG 3 cut(s) 98, 277, 294
FauNDI CATATG 1 cut(s) 80
Fnu4HI GCNGC 2 cut(s) 103, 265
Fsp4HI GCNGC 2 cut(s) 103, 265
FspBI CTAG 2 cut(s) 74, 233
GluI GCNGC 2 cut(s) 103, 265
HapII CCGG 2 cut(s) 257, 347
Hin1II CATG 3 cut(s) 102, 281, 298
HinfI GANTC 1 cut(s) 29
HpaII CCGG 2 cut(s) 257, 347
Hpy188I TCNGA 1 cut(s) 209
Hpy188III TCNNGA 1 cut(s) 116
HpyAV CCTTC 1 cut(s) 178
HpyCH4III ACNGT 1 cut(s) 215
HpyCH4IV ACGT 3 cut(s) 144, 218, 251
HpyCH4V TGCA 4 cut(s) 105, 169, 264, 288
HpyF10VI GCNNNNNNNGC 2 cut(s) 102, 327
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 3 cut(s) 144, 218, 251
Hsp92II CATG 3 cut(s) 102, 281, 298
Kzo9I GATC 1 cut(s) 156
Lsp1109I GCAGC 2 cut(s) 89, 276
MaeI CTAG 2 cut(s) 74, 233
MaeII ACGT 3 cut(s) 144, 218, 251
MaeIII GTNAC 1 cut(s) 349
MalI GATC 1 cut(s) 158
MboI GATC 1 cut(s) 156
MboII GAAGA 3 cut(s) 131, 134, 137
MfeI CAATTG 1 cut(s) 160
MluCI AATT 3 cut(s) 18, 45, 160
MlyI GAGTC 1 cut(s) 23
MmeI TCCRAC 1 cut(s) 187
MnlI CCTC 2 cut(s) 4, 43
Mph1103I ATGCAT 1 cut(s) 290
MroXI GAANNNNTTC 1 cut(s) 243
MslI CAYNNNNRTG 1 cut(s) 293
MspI CCGG 2 cut(s) 257, 347
MspR9I CCNGG 1 cut(s) 323
MunI CAATTG 1 cut(s) 160
MvaI CCWGG 1 cut(s) 323
MwoI GCNNNNNNNGC 2 cut(s) 102, 327
NdeI CATATG 1 cut(s) 80
NdeII GATC 1 cut(s) 156
NlaIII CATG 3 cut(s) 102, 281, 298
NsiI ATGCAT 1 cut(s) 290
NspI RCATGY 1 cut(s) 298
PciI ACATGT 1 cut(s) 294
PdmI GAANNNNTTC 1 cut(s) 243
Pfl23II CGTACG 1 cut(s) 248
PkrI GCNGC 2 cut(s) 104, 266
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
Ppu21I YACGTR 1 cut(s) 219
PscI ACATGT 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 321
PspGI CCWGG 1 cut(s) 321
PspLI CGTACG 1 cut(s) 248
PstI CTGCAG 2 cut(s) 107, 266
RsaI GTAC 3 cut(s) 179, 217, 250
RsaNI GTAC 3 cut(s) 178, 216, 249
RseI CAYNNNNRTG 1 cut(s) 293
SatI GCNGC 2 cut(s) 103, 265
Sau3AI GATC 1 cut(s) 156
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 1 cut(s) 323
SetI ASST 3 cut(s) 147, 221, 254
SfcI CTRYAG 2 cut(s) 103, 262
SmiMI CAYNNNNRTG 1 cut(s) 293
Sse9I AATT 3 cut(s) 18, 45, 160
SspI AATATT 1 cut(s) 242
SspMI CTAG 2 cut(s) 74, 233
StyD4I CCNGG 1 cut(s) 321
StyI CCWWGG 1 cut(s) 232
TaaI ACNGT 1 cut(s) 215
TaiI ACGT 3 cut(s) 147, 221, 254
TaqI TCGA 1 cut(s) 9
TasI AATT 3 cut(s) 18, 45, 160
TseI GCWGC 2 cut(s) 102, 264
TspDTI ATGAA 1 cut(s) 266
XceI RCATGY 1 cut(s) 298
XmaJI CCTAGG 1 cut(s) 232
XmnI GAANNNNTTC 1 cut(s) 243
XspI CTAG 2 cut(s) 74, 233
Zsp2I ATGCAT 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.