MD01G1149600.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001586
Physical Location & Seq
Forward (+)
25856135 .. 25859181
3047 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1149600.v1.1.491

Sequence Viewer

Length: 831 bp
ATGGCTCCACGAGCACGAGGCAGAAAGAAGGATACTCGAATGGATGCAGCCATTGATGCGACGCGTGAAATGGGTTTTGACGAGAAACTTGTTCGTGCTACGGTGAATGACCTACTGAAGGTTTATAAGGGAGATAATAGGACAGAGAATCCATGGCGATTTATCGAAGAGGGTTCTTATACGCTATTGGTTGAGCAACTTCTTGAGAAAGTAGAAGCCCATGAGGCTGCTGAAAAAAGGGATGCTGCCTCACAAGACAATAATGCACCACAAGACGATGCCGCTATGCAAGAAGACACTGGTATGCAAGAAGGCGCTGGTATGCAAGAAGATGTTGCTATGCAAGATGATGCTGCTCCGCTAGAAGATGTTGCTTTACTAGAAGATGCTTCTCCGCTAGACGATGCTGCACCACTAGACGATGCATTAGCTTCTGTGCCCTCAAACACTGCCATTCTATCTACTTGCGATAATGCTCCACAAGATGCTGGATCAGTGGCCGGAACTGCAAGGACTGCCATTTTATCTACCTGCTCAGAGGCTGTCAACATCACATTGCTGACTGAGGATCCTTGTGACTCTGCATCACATACTAATGGACCTATCCCGGCATCAATGATTAAAATGCCAGATGCCAAGGATGGTCTCCGAGTAGACAGTCTCCCTCCCCGGAGGTCTAGACCTTACTACGGCTGGGTCTGTAGCAGTGACGAGGAATATCCTGTGGAAGTGGCATCGTCTCCATTGTCAGAGTTGGTTAAATTGCTTATTAGGCCAGATAAGAAGAAAAAGCGGAAGTCGAGGTGGGACATGGGACCTGAAGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WIYLD

Protein Analysis

277

Amino Acids

30.08

Weight (kDa)

4.48

Isoelectric Point (pI)

52.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WIYLD PF10440 9 - 72 2.2e-19 Ubiquitin-binding WIYLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 126
Acc36I ACCTGC 1 cut(s) 539
AccI GTMKAC 1 cut(s) 654
AccII CGCG 1 cut(s) 64
AciI CCGC 4 cut(s) 282, 359, 395, 793
AclWI GGATC 3 cut(s) 499, 563, 576
AcoI YGGCCR 1 cut(s) 498
AcuI CTGAAG 1 cut(s) 137
AfiI CCNNNNNNNGG 2 cut(s) 118, 689
AflIII ACRYGT 1 cut(s) 62
AluBI AGCT 1 cut(s) 431
AluI AGCT 1 cut(s) 431
Alw21I GWGCWC 1 cut(s) 16
Alw26I GTCTC 3 cut(s) 650, 665, 744
AlwI GGATC 3 cut(s) 499, 563, 576
AlwNI CAGNNNCTG 1 cut(s) 542
AoxI GGCC 2 cut(s) 498, 773
ApeKI GCWGC 5 cut(s) 47, 227, 245, 353, 407
AspLEI GCGC 1 cut(s) 317
AspS9I GGNCC 2 cut(s) 599, 815
AsuC2I CCSGG 2 cut(s) 608, 670
AsuHPI GGTGA 1 cut(s) 115
AvaII GGWCC 2 cut(s) 599, 815
BaeGI GKGCMC 1 cut(s) 441
BamHI GGATCC 1 cut(s) 568
BauI CACGAG 2 cut(s) 9, 15
BbsI GAAGAC 1 cut(s) 300
Bbv12I GWGCWC 1 cut(s) 16
BbvI GCAGC 5 cut(s) 59, 214, 232, 340, 394
BccI CCATC 1 cut(s) 635
BceAI ACGGC 1 cut(s) 706
BciVI GTATCC 1 cut(s) 25
BcnI CCSGG 2 cut(s) 608, 670
BcoDI GTCTC 3 cut(s) 650, 665, 744
BfaI CTAG 5 cut(s) 362, 380, 398, 416, 678
BfmI CTRYAG 1 cut(s) 700
BfoI RGCGCY 1 cut(s) 318
BfuAI ACCTGC 1 cut(s) 539
BfuI GTATCC 1 cut(s) 25
BglI GCCNNNNNGGC 1 cut(s) 224
BisI GCNGC 6 cut(s) 48, 228, 246, 282, 354, 408
BlsI GCNGC 6 cut(s) 49, 229, 247, 283, 355, 409
Bme1390I CCNGG 2 cut(s) 608, 670
Bme18I GGWCC 2 cut(s) 599, 815
BmgT120I GGNCC 2 cut(s) 599, 815
BmiI GGNNCC 3 cut(s) 6, 570, 816
BmrFI CCNGG 2 cut(s) 608, 670
BpiI GAAGAC 1 cut(s) 300
BpuEI CTTGAG 1 cut(s) 224
BpuMI CCSGG 2 cut(s) 608, 670
BsaI GGTCTC 1 cut(s) 650
BsaJI CCNNGG 3 cut(s) 152, 636, 668
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 689
Bse1I ACTGG 1 cut(s) 304
Bse3DI GCAATG 1 cut(s) 554
BseDI CCNNGG 3 cut(s) 152, 636, 668
BseGI GGATG 3 cut(s) 49, 247, 646
BseLI CCNNNNNNNGG 2 cut(s) 118, 689
BseMI GCAATG 1 cut(s) 554
BseMII CTCAG 2 cut(s) 549, 555
BseNI ACTGG 1 cut(s) 304
BseSI GKGCMC 1 cut(s) 441
BseXI GCAGC 5 cut(s) 59, 214, 232, 340, 394
BseYI CCCAGC 1 cut(s) 693
BsgI GTGCAG 1 cut(s) 393
Bsh1236I CGCG 1 cut(s) 64
BshFI GGCC 2 cut(s) 500, 775
BsiHKAI GWGCWC 1 cut(s) 16
BsiSI CCGG 3 cut(s) 501, 608, 670
BslFI GGGAC 1 cut(s) 821
BslI CCNNNNNNNGG 2 cut(s) 118, 689
BsmAI GTCTC 3 cut(s) 650, 665, 744
BsmBI CGTCTC 1 cut(s) 744
BsmFI GGGAC 1 cut(s) 821
BsnI GGCC 2 cut(s) 500, 775
Bso31I GGTCTC 1 cut(s) 650
Bsp1286I GDGCHC 2 cut(s) 16, 441
Bsp143I GATC 2 cut(s) 491, 568
Bsp19I CCATGG 1 cut(s) 152
BspACI CCGC 4 cut(s) 282, 359, 395, 793
BspANI GGCC 2 cut(s) 500, 775
BspCNI CTCAG 2 cut(s) 548, 556
BspFNI CGCG 1 cut(s) 64
BspLI GGNNCC 3 cut(s) 6, 570, 816
BspMI ACCTGC 1 cut(s) 539
BspPI GGATC 3 cut(s) 499, 563, 576
BspTNI GGTCTC 1 cut(s) 650
BsrDI GCAATG 1 cut(s) 554
BsrI ACTGG 1 cut(s) 304
BssECI CCNNGG 3 cut(s) 152, 636, 668
BssMI GATC 2 cut(s) 491, 568
BssSI CACGAG 2 cut(s) 9, 15
BssT1I CCWWGG 2 cut(s) 152, 636
Bst2BI CACGAG 2 cut(s) 9, 15
Bst4CI ACNGT 2 cut(s) 103, 659
Bst6I CTCTTC 1 cut(s) 162
BstAPI GCANNNNNTGC 1 cut(s) 515
BstDEI CTNAG 2 cut(s) 535, 564
BstDSI CCRYGG 1 cut(s) 152
BstENI CCTNNNNNAGG 1 cut(s) 116
BstF5I GGATG 3 cut(s) 49, 247, 646
BstFNI CGCG 1 cut(s) 64
BstH2I RGCGCY 1 cut(s) 318
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 2 cut(s) 494, 571
BstMAI GTCTC 3 cut(s) 650, 665, 744
BstMBI GATC 2 cut(s) 491, 568
BstMWI GCNNNNNNNGC 6 cut(s) 11, 56, 224, 506, 515, 772
BstSCI CCNGG 2 cut(s) 606, 668
BstSFI CTRYAG 1 cut(s) 700
BstSLI GKGCMC 1 cut(s) 441
BstUI CGCG 1 cut(s) 64
BstV1I GCAGC 5 cut(s) 59, 214, 232, 340, 394
BstV2I GAAGAC 1 cut(s) 300
BstX2I RGATCY 1 cut(s) 568
BstYI RGATCY 1 cut(s) 568
BsuI GTATCC 1 cut(s) 25
BsuRI GGCC 2 cut(s) 500, 775
BtgI CCRYGG 1 cut(s) 152
BtsCI GGATG 3 cut(s) 49, 247, 646
BtsI GCAGTG 2 cut(s) 447, 712
BtsIMutI CAGTG 4 cut(s) 297, 447, 501, 712
BveI ACCTGC 1 cut(s) 539
CaiI CAGNNNCTG 1 cut(s) 542
CfoI GCGC 1 cut(s) 317
Cfr13I GGNCC 2 cut(s) 599, 815
CseI GACGC 1 cut(s) 70
CviAII CATG 3 cut(s) 153, 221, 811
CviJI RGCY 9 cut(s) 5, 50, 218, 227, 431, 500, 542, 693, 775
CviKI_1 RGCY 9 cut(s) 5, 50, 218, 227, 431, 500, 542, 693, 775
DdeI CTNAG 2 cut(s) 535, 564
DpnI GATC 2 cut(s) 493, 570
DpnII GATC 2 cut(s) 491, 568
EaeI YGGCCR 1 cut(s) 498
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
Eco130I CCWWGG 2 cut(s) 152, 636
Eco31I GGTCTC 1 cut(s) 650
Eco47I GGWCC 2 cut(s) 599, 815
Eco57I CTGAAG 1 cut(s) 137
EcoNI CCTNNNNNAGG 1 cut(s) 116
EcoO109I RGGNCCY 1 cut(s) 815
EcoT14I CCWWGG 2 cut(s) 152, 636
EcoT22I ATGCAT 1 cut(s) 427
ErhI CCWWGG 2 cut(s) 152, 636
Esp3I CGTCTC 1 cut(s) 744
FaeI CATG 3 cut(s) 156, 224, 814
FaqI GGGAC 1 cut(s) 821
FatI CATG 3 cut(s) 152, 220, 810
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 6 cut(s) 48, 228, 246, 282, 354, 408
FokI GGATG 3 cut(s) 56, 254, 653
Fsp4HI GCNGC 6 cut(s) 48, 228, 246, 282, 354, 408
FspBI CTAG 5 cut(s) 362, 380, 398, 416, 678
GlaI GCGC 1 cut(s) 316
GluI GCNGC 6 cut(s) 48, 228, 246, 282, 354, 408
GsaI CCCAGC 1 cut(s) 697
HaeII RGCGCY 1 cut(s) 318
HaeIII GGCC 2 cut(s) 500, 775
HapII CCGG 3 cut(s) 501, 608, 670
HgaI GACGC 1 cut(s) 70
HhaI GCGC 1 cut(s) 317
Hin1II CATG 3 cut(s) 156, 224, 814
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HinfI GANTC 2 cut(s) 148, 578
HpaII CCGG 3 cut(s) 501, 608, 670
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 2 cut(s) 547, 655
Hpy188I TCNGA 3 cut(s) 538, 650, 751
Hpy188III TCNNGA 2 cut(s) 203, 678
Hpy8I GTNNAC 2 cut(s) 547, 655
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 3 cut(s) 22, 112, 305
HpyCH4III ACNGT 2 cut(s) 103, 659
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 56, 224, 506, 515, 772
HpyF3I CTNAG 2 cut(s) 535, 564
Hsp92II CATG 3 cut(s) 156, 224, 814
HspAI GCGC 1 cut(s) 315
Kzo9I GATC 2 cut(s) 491, 568
LmnI GCTCC 3 cut(s) 10, 361, 481
Lsp1109I GCAGC 5 cut(s) 59, 214, 232, 340, 394
MaeI CTAG 5 cut(s) 362, 380, 398, 416, 678
MaeIII GTNAC 2 cut(s) 575, 707
MalI GATC 2 cut(s) 493, 570
MboI GATC 2 cut(s) 491, 568
MboII GAAGA 6 cut(s) 179, 305, 341, 377, 395, 796
MflI RGATCY 1 cut(s) 568
MhlI GDGCHC 2 cut(s) 16, 441
MluCI AATT 1 cut(s) 761
MluI ACGCGT 1 cut(s) 62
MlyI GAGTC 1 cut(s) 572
Mph1103I ATGCAT 1 cut(s) 427
MseI TTAA 2 cut(s) 621, 759
MslI CAYNNNNRTG 2 cut(s) 302, 594
MspI CCGG 3 cut(s) 501, 608, 670
MspR9I CCNGG 2 cut(s) 608, 670
MvnI CGCG 1 cut(s) 64
MwoI GCNNNNNNNGC 6 cut(s) 11, 56, 224, 506, 515, 772
NciI CCSGG 2 cut(s) 608, 670
NcoI CCATGG 1 cut(s) 152
NdeII GATC 2 cut(s) 491, 568
NlaIII CATG 3 cut(s) 156, 224, 814
NlaIV GGNNCC 3 cut(s) 6, 570, 816
NmuCI GTSAC 2 cut(s) 575, 707
NsiI ATGCAT 1 cut(s) 427
PfeI GAWTC 1 cut(s) 148
PkrI GCNGC 6 cut(s) 49, 229, 247, 283, 355, 409
PleI GAGTC 1 cut(s) 572
PpsI GAGTC 1 cut(s) 572
PpuMI RGGWCCY 1 cut(s) 815
PsiI TTATAA 1 cut(s) 126
Psp5II RGGWCCY 1 cut(s) 815
PspFI CCCAGC 1 cut(s) 693
PspN4I GGNNCC 3 cut(s) 6, 570, 816
PspPI GGNCC 2 cut(s) 599, 815
PspPPI RGGWCCY 1 cut(s) 815
PstNI CAGNNNCTG 1 cut(s) 542
PsuI RGATCY 1 cut(s) 568
RseI CAYNNNNRTG 2 cut(s) 302, 594
SaqAI TTAA 2 cut(s) 621, 759
SatI GCNGC 6 cut(s) 48, 228, 246, 282, 354, 408
Sau3AI GATC 2 cut(s) 491, 568
Sau96I GGNCC 2 cut(s) 599, 815
SchI GAGTC 1 cut(s) 572
ScrFI CCNGG 2 cut(s) 608, 670
SduI GDGCHC 2 cut(s) 16, 441
SetI ASST 9 cut(s) 114, 123, 433, 533, 604, 677, 685, 806, 820
SfcI CTRYAG 1 cut(s) 700
SinI GGWCC 2 cut(s) 599, 815
SmiMI CAYNNNNRTG 2 cut(s) 302, 594
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 1 cut(s) 761
SsiI CCGC 4 cut(s) 282, 359, 395, 793
SspMI CTAG 5 cut(s) 362, 380, 398, 416, 678
StyD4I CCNGG 2 cut(s) 606, 668
StyI CCWWGG 2 cut(s) 152, 636
TaaI ACNGT 2 cut(s) 103, 659
TaqI TCGA 3 cut(s) 37, 165, 800
TasI AATT 1 cut(s) 761
TauI GCSGC 1 cut(s) 284
TfiI GAWTC 1 cut(s) 148
Tru1I TTAA 2 cut(s) 621, 759
Tru9I TTAA 2 cut(s) 621, 759
TscAI CASTG 4 cut(s) 304, 454, 501, 712
TseFI GTSAC 2 cut(s) 575, 707
TseI GCWGC 5 cut(s) 47, 227, 245, 353, 407
Tsp45I GTSAC 2 cut(s) 575, 707
TspRI CASTG 4 cut(s) 304, 454, 501, 712
VpaK11BI GGWCC 2 cut(s) 599, 815
XagI CCTNNNNNAGG 1 cut(s) 116
XbaI TCTAGA 1 cut(s) 677
XmiI GTMKAC 1 cut(s) 654
XspI CTAG 5 cut(s) 362, 380, 398, 416, 678
Zsp2I ATGCAT 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.