MD17G1067400.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001586
Physical Location & Seq
Forward (+)
5443083 .. 5445329
2247 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1067400.v1.1.491

Sequence Viewer

Length: 492 bp
ATGGCCCCAAGACCAAGAGGACGACCCAGGAAGCGCAATACTCGAATGTGTGCTGCGATTGATGCTATGAAACCCATGGGTTTTTCGGAGAATTTAGTTTGTGATACAGTGAAAGAACTACTGGAAGTTTATGGGGAAGATGGTGATACAACCGGATGGCCCTTTATCGAAGAAGGTGCTTACTCCCTCCTGATTGAAACAATTCTTGAGAAACAAGGCGGTTCTGAAAACAAGGATGCTTCTCTTCTGGATGATGCAGGAAGTGGAAATGGCGTCGAAGCGTCAGCAGCTAAGCTCTCAATCACAGTTTTTTGCCCGGATAGTGTTGCACCGCGGAGGCTTAGACCTTTGTACGGCTGGATTTGTAGGGTTGGTGATGATGATAATAAGCTACCTGTGGAACTAGCACCGGTTCCGTCGTTGGAGTTTGTTGCCGCTTTATTGCACAGATGGGAGAAGAAAGTGCAAGTCAAGGTGGAATGTGAGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WIYLD

Protein Analysis

164

Amino Acids

17.91

Weight (kDa)

5.04

Isoelectric Point (pI)

46.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WIYLD PF10440 10 - 72 3.6e-20 Ubiquitin-binding WIYLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 334
AciI CCGC 4 cut(s) 219, 332, 334, 435
AcsI RAATTY 1 cut(s) 91
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 1 cut(s) 353
AfiI CCNNNNNNNGG 1 cut(s) 353
AgeI ACCGGT 1 cut(s) 409
AgsI TTSAA 1 cut(s) 197
AjnI CCWGG 1 cut(s) 26
AluBI AGCT 3 cut(s) 290, 295, 391
AluI AGCT 3 cut(s) 290, 295, 391
AoxI GGCC 3 cut(s) 3, 158, 486
ApeKI GCWGC 2 cut(s) 53, 287
ApoI RAATTY 1 cut(s) 91
AsiGI ACCGGT 1 cut(s) 409
Asp700I GAANNNNTTC 1 cut(s) 201
AspLEI GCGC 1 cut(s) 36
AspS9I GGNCC 2 cut(s) 4, 159
AsuC2I CCSGG 1 cut(s) 317
AsuHPI GGTGA 2 cut(s) 155, 386
BbvI GCAGC 2 cut(s) 40, 299
BccI CCATC 3 cut(s) 134, 150, 444
BceAI ACGGC 1 cut(s) 370
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BciT130I CCWGG 1 cut(s) 28
BcnI CCSGG 1 cut(s) 317
BfaI CTAG 1 cut(s) 404
BisI GCNGC 3 cut(s) 54, 288, 435
BlpI GCTNAGC 1 cut(s) 291
BlsI GCNGC 3 cut(s) 55, 289, 436
Bme1390I CCNGG 2 cut(s) 28, 317
BmgT120I GGNCC 2 cut(s) 4, 159
BmiI GGNNCC 2 cut(s) 6, 414
BmrFI CCNGG 2 cut(s) 28, 317
BmsI GCATC 3 cut(s) 52, 226, 244
Bpu1102I GCTNAGC 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 227
BpuMI CCSGG 1 cut(s) 317
BsaHI GRCGYC 1 cut(s) 273
BsaJI CCNNGG 3 cut(s) 26, 75, 332
BsaWI WCCGGW 2 cut(s) 152, 409
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse118I RCCGGY 1 cut(s) 409
Bse1I ACTGG 1 cut(s) 126
BseBI CCWGG 1 cut(s) 28
BseDI CCNNGG 3 cut(s) 26, 75, 332
BseGI GGATG 3 cut(s) 161, 241, 256
BseLI CCNNNNNNNGG 1 cut(s) 353
BseNI ACTGG 1 cut(s) 126
BseXI GCAGC 2 cut(s) 40, 299
Bsh1236I CGCG 1 cut(s) 334
BshFI GGCC 3 cut(s) 5, 160, 488
BshTI ACCGGT 1 cut(s) 409
BsiSI CCGG 3 cut(s) 153, 317, 410
BslI CCNNNNNNNGG 1 cut(s) 353
BsnI GGCC 3 cut(s) 5, 160, 488
Bsp1720I GCTNAGC 1 cut(s) 291
Bsp19I CCATGG 1 cut(s) 75
BspACI CCGC 4 cut(s) 219, 332, 334, 435
BspANI GGCC 3 cut(s) 5, 160, 488
BspFNI CGCG 1 cut(s) 334
BspLI GGNNCC 2 cut(s) 6, 414
BsrFI RCCGGY 1 cut(s) 409
BsrI ACTGG 1 cut(s) 126
BssAI RCCGGY 1 cut(s) 409
BssECI CCNNGG 3 cut(s) 26, 75, 332
BssNI GRCGYC 1 cut(s) 273
BssT1I CCWWGG 1 cut(s) 75
Bst2UI CCWGG 1 cut(s) 28
Bst4CI ACNGT 2 cut(s) 109, 307
Bst6I CTCTTC 1 cut(s) 249
BstACI GRCGYC 1 cut(s) 273
BstDEI CTNAG 2 cut(s) 291, 341
BstDSI CCRYGG 2 cut(s) 75, 332
BstF5I GGATG 3 cut(s) 161, 241, 256
BstFNI CGCG 1 cut(s) 334
BstHHI GCGC 1 cut(s) 36
BstMWI GCNNNNNNNGC 2 cut(s) 62, 287
BstNI CCWGG 1 cut(s) 28
BstSCI CCNGG 2 cut(s) 26, 315
BstUI CGCG 1 cut(s) 334
BstV1I GCAGC 2 cut(s) 40, 299
BsuRI GGCC 3 cut(s) 5, 160, 488
BtgI CCRYGG 2 cut(s) 75, 332
BtsCI GGATG 3 cut(s) 161, 241, 256
BtsIMutI CAGTG 1 cut(s) 114
CfoI GCGC 1 cut(s) 36
Cfr10I RCCGGY 1 cut(s) 409
Cfr13I GGNCC 2 cut(s) 4, 159
Cfr42I CCGCGG 1 cut(s) 335
CseI GACGC 2 cut(s) 262, 270
Csp6I GTAC 1 cut(s) 352
CspAI ACCGGT 1 cut(s) 409
CviAII CATG 1 cut(s) 76
CviJI RGCY 8 cut(s) 5, 160, 290, 295, 340, 357, 391, 488
CviKI_1 RGCY 8 cut(s) 5, 160, 290, 295, 340, 357, 391, 488
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 2 cut(s) 291, 341
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 75
Eco147I AGGCCT 1 cut(s) 488
EcoRII CCWGG 1 cut(s) 26
EcoT14I CCWWGG 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 1 cut(s) 79
FaiI YATR 3 cut(s) 68, 77, 132
FatI CATG 1 cut(s) 75
Fnu4HI GCNGC 3 cut(s) 54, 288, 435
FokI GGATG 3 cut(s) 168, 248, 263
Fsp4HI GCNGC 3 cut(s) 54, 288, 435
FspBI CTAG 1 cut(s) 404
GlaI GCGC 1 cut(s) 35
GluI GCNGC 3 cut(s) 54, 288, 435
HaeIII GGCC 3 cut(s) 5, 160, 488
HapII CCGG 3 cut(s) 153, 317, 410
HgaI GACGC 2 cut(s) 262, 270
HhaI GCGC 1 cut(s) 36
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 1 cut(s) 79
Hin6I GCGC 1 cut(s) 34
HinP1I GCGC 1 cut(s) 34
HpaII CCGG 3 cut(s) 153, 317, 410
HphI GGTGA 2 cut(s) 155, 386
Hpy188I TCNGA 2 cut(s) 88, 226
Hpy188III TCNNGA 3 cut(s) 190, 206, 248
Hpy99I CGWCG 2 cut(s) 278, 421
HpyAV CCTTC 1 cut(s) 167
HpyCH4III ACNGT 2 cut(s) 109, 307
HpyCH4V TGCA 4 cut(s) 257, 329, 445, 466
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 287
HpyF3I CTNAG 2 cut(s) 291, 341
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 1 cut(s) 79
HspAI GCGC 1 cut(s) 34
KspI CCGCGG 1 cut(s) 335
Lsp1109I GCAGC 2 cut(s) 40, 299
LweI GCATC 3 cut(s) 52, 226, 244
MaeI CTAG 1 cut(s) 404
MboII GAAGA 4 cut(s) 149, 182, 236, 469
MluCI AATT 2 cut(s) 91, 201
MmeI TCCRAC 1 cut(s) 402
MnlI CCTC 4 cut(s) 11, 197, 330, 478
MroXI GAANNNNTTC 1 cut(s) 201
MspA1I CMGCKG 1 cut(s) 334
MspI CCGG 3 cut(s) 153, 317, 410
MspR9I CCNGG 2 cut(s) 28, 317
MvaI CCWGG 1 cut(s) 28
MvnI CGCG 1 cut(s) 334
MwoI GCNNNNNNNGC 2 cut(s) 62, 287
NciI CCSGG 1 cut(s) 317
NcoI CCATGG 1 cut(s) 75
NlaIII CATG 1 cut(s) 79
NlaIV GGNNCC 2 cut(s) 6, 414
PceI AGGCCT 1 cut(s) 488
PdmI GAANNNNTTC 1 cut(s) 201
PinAI ACCGGT 1 cut(s) 409
PkrI GCNGC 3 cut(s) 55, 289, 436
Psp6I CCWGG 1 cut(s) 26
PspGI CCWGG 1 cut(s) 26
PspN4I GGNNCC 2 cut(s) 6, 414
PspPI GGNCC 2 cut(s) 4, 159
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
SacII CCGCGG 1 cut(s) 335
SatI GCNGC 3 cut(s) 54, 288, 435
Sau96I GGNCC 2 cut(s) 4, 159
ScrFI CCNGG 2 cut(s) 28, 317
SetI ASST 7 cut(s) 178, 292, 297, 349, 393, 397, 477
SfaNI GCATC 3 cut(s) 52, 226, 244
Sfr303I CCGCGG 1 cut(s) 335
SgrBI CCGCGG 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 2 cut(s) 91, 201
SseBI AGGCCT 1 cut(s) 488
SsiI CCGC 4 cut(s) 219, 332, 334, 435
SspMI CTAG 1 cut(s) 404
StuI AGGCCT 1 cut(s) 488
StyD4I CCNGG 2 cut(s) 26, 315
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 2 cut(s) 109, 307
TaqI TCGA 3 cut(s) 43, 168, 276
TasI AATT 2 cut(s) 91, 201
TauI GCSGC 1 cut(s) 437
TscAI CASTG 1 cut(s) 114
TseI GCWGC 2 cut(s) 53, 287
TspDTI ATGAA 1 cut(s) 83
TspGWI ACGGA 1 cut(s) 405
TspRI CASTG 1 cut(s) 114
XapI RAATTY 1 cut(s) 91
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.