Rroxscaffold_4G00287390

Ubiquitin-binding WIYLD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001586
Physical Location & Seq
Reverse (-)
8418011 .. 8421312
3302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00287390.1

Sequence Viewer

Length: 582 bp
ATGGCTCCAAGAAGAGGTCGAGGTGGACCACCCAAGACTTCTCGTATGGCTGCCGCCGTTGAGGCCATGGCGGACATGGGTTTCGAGCCCAAACTGGTCCGTCAGAAGGTGAACCGGTTACTAAAAAAAGACCTTTATGGAGAAGAGGGCTGGCGTTTTATTGAAGAAGGTTCTTACAGTGTACTACTTGAAGCCCTTCTGGAGGAGCAAACAGATGGTGATGAAAATGAGAATGCATTTCCGGAAAAATCAAAAGGTGGAAAAGATGCTGAAACTGCAGGAGCTGGACCCTCCTCGAGCAAGGATGCTTCTCTGAAAAAATCAACCTGTGGAAATGACCCTGAAACTTCAGAAGCTGGACTATTAAGCAAGGATGTATCTTTACAAACTGATGCAAGAGGTGTAAATGATACTGAAACTTCAGGAGCTGGACTATCCATAATCAAGGATGTGTCTCCACAAACTGATGCAAGAGGTGTAAATGATGCTGAATCATCAGGAGCTGGACCTTCCTCAAGCAAGAAAATGATCCAAGTGGTGGAAGTGAGGTTGAAACATCAGGACCTGTACCCTCCTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WIYLD

Protein Analysis

193

Amino Acids

20.51

Weight (kDa)

5.03

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WIYLD PF10440 13 - 70 3.1e-17 Ubiquitin-binding WIYLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 538
AccIII TCCGGA 1 cut(s) 241
AciI CCGC 2 cut(s) 54, 71
AclWI GGATC 1 cut(s) 523
AcuI CTGAAG 2 cut(s) 333, 405
AfaI GTAC 2 cut(s) 183, 569
AfiI CCNNNNNNNGG 4 cut(s) 14, 106, 202, 538
AgeI ACCGGT 1 cut(s) 114
AgsI TTSAA 3 cut(s) 164, 191, 553
AluBI AGCT 4 cut(s) 284, 356, 428, 503
AluI AGCT 4 cut(s) 284, 356, 428, 503
Alw26I GTCTC 1 cut(s) 459
AlwI GGATC 1 cut(s) 523
AlwNI CAGNNNCTG 5 cut(s) 284, 356, 428, 503, 565
Ama87I CYCGRG 1 cut(s) 295
Aor13HI TCCGGA 1 cut(s) 241
AoxI GGCC 1 cut(s) 63
ApeKI GCWGC 1 cut(s) 50
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
AsiGI ACCGGT 1 cut(s) 114
Asp700I GAANNNNTTC 1 cut(s) 195
AspS9I GGNCC 5 cut(s) 26, 97, 287, 506, 562
AsuHPI GGTGA 2 cut(s) 121, 230
AvaI CYCGRG 1 cut(s) 295
AvaII GGWCC 5 cut(s) 26, 97, 287, 506, 562
BanII GRGCYC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 37
BccI CCATC 1 cut(s) 209
BceAI ACGGC 1 cut(s) 41
BcoDI GTCTC 1 cut(s) 459
BfmI CTRYAG 1 cut(s) 276
BglI GCCNNNNNGGC 1 cut(s) 62
BisI GCNGC 2 cut(s) 51, 54
BlsI GCNGC 2 cut(s) 52, 55
Bme18I GGWCC 5 cut(s) 26, 97, 287, 506, 562
BmeT110I CYCGRG 1 cut(s) 295
BmgT120I GGNCC 5 cut(s) 26, 97, 287, 506, 562
BmiI GGNNCC 2 cut(s) 6, 289
BmsI GCATC 5 cut(s) 256, 295, 382, 457, 475
BpmI CTGGAG 1 cut(s) 221
BpuEI CTTGAG 1 cut(s) 499
BsaJI CCNNGG 1 cut(s) 66
BsaWI WCCGGW 2 cut(s) 114, 241
Bsc4I CCNNNNNNNGG 4 cut(s) 14, 106, 202, 538
Bse118I RCCGGY 1 cut(s) 114
Bse1I ACTGG 1 cut(s) 99
BseAI TCCGGA 1 cut(s) 241
BseDI CCNNGG 1 cut(s) 66
BseGI GGATG 3 cut(s) 310, 379, 454
BseLI CCNNNNNNNGG 4 cut(s) 14, 106, 202, 538
BseNI ACTGG 1 cut(s) 99
BseRI GAGGAG 3 cut(s) 218, 283, 564
BseXI GCAGC 1 cut(s) 37
BshFI GGCC 1 cut(s) 65
BshTI ACCGGT 1 cut(s) 114
BsiHKCI CYCGRG 1 cut(s) 295
BsiSI CCGG 2 cut(s) 115, 242
BslI CCNNNNNNNGG 4 cut(s) 14, 106, 202, 538
BsmAI GTCTC 1 cut(s) 459
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 1 cut(s) 65
BsoBI CYCGRG 1 cut(s) 295
Bsp1286I GDGCHC 1 cut(s) 90
Bsp13I TCCGGA 1 cut(s) 241
Bsp143I GATC 1 cut(s) 528
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 2 cut(s) 54, 71
BspANI GGCC 1 cut(s) 65
BspEI TCCGGA 1 cut(s) 241
BspLI GGNNCC 2 cut(s) 6, 289
BspMAI CTGCAG 1 cut(s) 280
BspPI GGATC 1 cut(s) 523
BsrFI RCCGGY 1 cut(s) 114
BsrI ACTGG 1 cut(s) 99
BssAI RCCGGY 1 cut(s) 114
BssECI CCNNGG 1 cut(s) 66
BssMI GATC 1 cut(s) 528
BssT1I CCWWGG 1 cut(s) 66
Bst4CI ACNGT 1 cut(s) 179
Bst6I CTCTTC 2 cut(s) 7, 138
BstC8I GCNNGC 1 cut(s) 152
BstDSI CCRYGG 1 cut(s) 66
BstENI CCTNNNNNAGG 1 cut(s) 200
BstF5I GGATG 3 cut(s) 310, 379, 454
BstKTI GATC 1 cut(s) 531
BstMAI GTCTC 1 cut(s) 459
BstMBI GATC 1 cut(s) 528
BstMWI GCNNNNNNNGC 2 cut(s) 62, 275
BstSFI CTRYAG 1 cut(s) 276
BstV1I GCAGC 1 cut(s) 37
BsuRI GGCC 1 cut(s) 65
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 3 cut(s) 310, 379, 454
BtsIMutI CAGTG 1 cut(s) 184
Cac8I GCNNGC 1 cut(s) 152
CaiI CAGNNNCTG 5 cut(s) 284, 356, 428, 503, 565
Cfr10I RCCGGY 1 cut(s) 114
Cfr13I GGNCC 5 cut(s) 26, 97, 287, 506, 562
Csp6I GTAC 2 cut(s) 182, 568
CspAI ACCGGT 1 cut(s) 114
CviAII CATG 2 cut(s) 67, 76
CviQI GTAC 2 cut(s) 182, 568
DpnI GATC 1 cut(s) 530
DpnII GATC 1 cut(s) 528
Eam1104I CTCTTC 2 cut(s) 7, 138
EarI CTCTTC 2 cut(s) 7, 138
EciI GGCGGA 1 cut(s) 86
Eco130I CCWWGG 1 cut(s) 66
Eco24I GRGCYC 1 cut(s) 90
Eco47I GGWCC 5 cut(s) 26, 97, 287, 506, 562
Eco57I CTGAAG 2 cut(s) 333, 405
Eco88I CYCGRG 1 cut(s) 295
EcoNI CCTNNNNNAGG 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 562
EcoT14I CCWWGG 1 cut(s) 66
EcoT22I ATGCAT 1 cut(s) 238
EcoT38I GRGCYC 1 cut(s) 90
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 2 cut(s) 70, 79
FaiI YATR 5 cut(s) 47, 68, 77, 138, 440
FatI CATG 2 cut(s) 66, 75
Fnu4HI GCNGC 2 cut(s) 51, 54
FokI GGATG 3 cut(s) 317, 386, 461
FriOI GRGCYC 1 cut(s) 90
Fsp4HI GCNGC 2 cut(s) 51, 54
GluI GCNGC 2 cut(s) 51, 54
GsuI CTGGAG 1 cut(s) 221
HaeIII GGCC 1 cut(s) 65
HapII CCGG 2 cut(s) 115, 242
Hin1II CATG 2 cut(s) 70, 79
HinfI GANTC 1 cut(s) 491
HpaII CCGG 2 cut(s) 115, 242
HphI GGTGA 2 cut(s) 121, 230
Hpy166II GTNNAC 3 cut(s) 26, 112, 182
Hpy188I TCNGA 3 cut(s) 105, 315, 352
Hpy188III TCNNGA 5 cut(s) 200, 242, 423, 498, 560
Hpy8I GTNNAC 3 cut(s) 26, 112, 182
HpyAV CCTTC 4 cut(s) 100, 161, 206, 519
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4V TGCA 4 cut(s) 236, 278, 395, 470
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 275
Hsp92II CATG 2 cut(s) 70, 79
Kpn2I TCCGGA 1 cut(s) 241
Kzo9I GATC 1 cut(s) 528
LmnI GCTCC 5 cut(s) 10, 205, 281, 425, 500
Lsp1109I GCAGC 1 cut(s) 37
LweI GCATC 5 cut(s) 256, 295, 382, 457, 475
MaeIII GTNAC 1 cut(s) 117
MalI GATC 1 cut(s) 530
MboI GATC 1 cut(s) 528
MboII GAAGA 3 cut(s) 24, 155, 176
MhlI GDGCHC 1 cut(s) 90
Mph1103I ATGCAT 1 cut(s) 238
MroI TCCGGA 1 cut(s) 241
MroXI GAANNNNTTC 1 cut(s) 195
MseI TTAA 2 cut(s) 365, 580
MspI CCGG 2 cut(s) 115, 242
Mva1269I GAATGC 1 cut(s) 238
MwoI GCNNNNNNNGC 2 cut(s) 62, 275
NcoI CCATGG 1 cut(s) 66
NdeII GATC 1 cut(s) 528
NlaIII CATG 2 cut(s) 70, 79
NlaIV GGNNCC 2 cut(s) 6, 289
NsiI ATGCAT 1 cut(s) 238
PaeR7I CTCGAG 1 cut(s) 295
PctI GAATGC 1 cut(s) 238
PdmI GAANNNNTTC 1 cut(s) 195
PfeI GAWTC 1 cut(s) 491
PflMI CCANNNNNTGG 1 cut(s) 538
PinAI ACCGGT 1 cut(s) 114
PkrI GCNGC 2 cut(s) 52, 55
PpuMI RGGWCCY 1 cut(s) 562
Psp5II RGGWCCY 1 cut(s) 562
PspN4I GGNNCC 2 cut(s) 6, 289
PspPI GGNCC 5 cut(s) 26, 97, 287, 506, 562
PspPPI RGGWCCY 1 cut(s) 562
PspXI VCTCGAGB 1 cut(s) 295
PstI CTGCAG 1 cut(s) 280
PstNI CAGNNNCTG 5 cut(s) 284, 356, 428, 503, 565
RsaI GTAC 2 cut(s) 183, 569
RsaNI GTAC 2 cut(s) 182, 568
SaqAI TTAA 2 cut(s) 365, 580
SatI GCNGC 2 cut(s) 51, 54
Sau3AI GATC 1 cut(s) 528
Sau96I GGNCC 5 cut(s) 26, 97, 287, 506, 562
SduI GDGCHC 1 cut(s) 90
SfaNI GCATC 5 cut(s) 256, 295, 382, 457, 475
SfcI CTRYAG 1 cut(s) 276
Sfr274I CTCGAG 1 cut(s) 295
SinI GGWCC 5 cut(s) 26, 97, 287, 506, 562
SlaI CTCGAG 1 cut(s) 295
SmlI CTYRAG 2 cut(s) 295, 514
SmoI CTYRAG 2 cut(s) 295, 514
SsiI CCGC 2 cut(s) 54, 71
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 1 cut(s) 179
TaqI TCGA 3 cut(s) 19, 84, 296
TatI WGTACW 1 cut(s) 181
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 1 cut(s) 491
Tru1I TTAA 2 cut(s) 365, 580
Tru9I TTAA 2 cut(s) 365, 580
TscAI CASTG 1 cut(s) 184
TseI GCWGC 1 cut(s) 50
TspDTI ATGAA 1 cut(s) 237
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 1 cut(s) 184
Van91I CCANNNNNTGG 1 cut(s) 538
VpaK11BI GGWCC 5 cut(s) 26, 97, 287, 506, 562
XagI CCTNNNNNAGG 1 cut(s) 200
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
XhoI CTCGAG 1 cut(s) 295
XmnI GAANNNNTTC 1 cut(s) 195
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.