Rorug01G0352200

Ubiquitin-binding WIYLD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001586
Physical Location & Seq
Forward (+)
46918253 .. 46918816
564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0352200.1

Sequence Viewer

Length: 564 bp
ATGCCAAAGCTTCCAATGAAGACAATAAGCATTTTGAGTATTTCTTCACTAGTCTTCTTCATTTCTCTGCAAACCAATGCAATGCATCCTATCAACCATGGCTTCCAAGGAGCCAAACCAAACTGCACCTACTCCATTGAGATTGAGACAACCTGTGCACAATCTGCCGAAACCACAGACCATGTCAGCGTCAGATTCAGCGACTCCAAGGGTGATCTGATCATAGTGAAGCATCTGAACGATCCTAAGCTCTTGTATGCTCCGAAAGGTGGTTCAAAACATGGAGGGTATGGCGGGTTTGGAAGATGTGCCATAGACGTGTTTGAGGCCAGTGGACCATGCATGAGCTCAAGGGTGTGCTATCTGTACCTGAAGAAAGTCGGATCAGATGACTGGCGACCTGGCTGGGTGAAGGTGCTACATCGACAAGATGGTGGCCATGAAGTACCAGTTTCTTATGTGTTCTATTTTAGGACATTTGTTCCAGAAAACGTTTGGTATGGCTTTGATTATTGTCACTCCAAGGGTGGTCTTGTACCTCATGTTGTCAGTTTTGACGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WIYLD

Protein Analysis

187

Amino Acids

20.78

Weight (kDa)

7.67

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATS3 PF06232 38 - 172 3.5e-36 Embryo-specific protein 3, (ATS3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 294
AclI AACGTT 1 cut(s) 492
AclWI GGATC 2 cut(s) 236, 391
AcoI YGGCCR 1 cut(s) 436
AcuI CTGAAG 1 cut(s) 392
AfaI GTAC 3 cut(s) 368, 447, 537
AfiI CCNNNNNNNGG 1 cut(s) 269
AflIII ACRYGT 1 cut(s) 318
AgsI TTSAA 1 cut(s) 276
AhlI ACTAGT 1 cut(s) 49
AjiI CACGTC 1 cut(s) 319
AjnI CCWGG 1 cut(s) 400
AluBI AGCT 3 cut(s) 10, 250, 348
AluI AGCT 3 cut(s) 10, 250, 348
Alw21I GWGCWC 2 cut(s) 160, 350
Alw26I GTCTC 1 cut(s) 140
Alw44I GTGCAC 1 cut(s) 156
AlwI GGATC 2 cut(s) 236, 391
AoxI GGCC 2 cut(s) 327, 436
ApaLI GTGCAC 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 335
AsuHPI GGTGA 2 cut(s) 224, 421
AvaII GGWCC 1 cut(s) 335
BaeGI GKGCMC 1 cut(s) 160
BalI TGGCCA 1 cut(s) 438
BanII GRGCYC 1 cut(s) 350
BbsI GAAGAC 2 cut(s) 26, 46
Bbv12I GWGCWC 2 cut(s) 160, 350
BccI CCATC 1 cut(s) 425
BciT130I CCWGG 1 cut(s) 402
BclI TGATCA 1 cut(s) 219
BcoDI GTCTC 1 cut(s) 140
BcuI ACTAGT 1 cut(s) 49
BfaI CTAG 2 cut(s) 50, 562
Bme1390I CCNGG 1 cut(s) 402
Bme18I GGWCC 1 cut(s) 335
BmgBI CACGTC 1 cut(s) 319
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 1 cut(s) 112
BmrFI CCNGG 1 cut(s) 402
BmsI GCATC 2 cut(s) 94, 241
BpiI GAAGAC 2 cut(s) 26, 46
Bpu10I CCTNAGC 1 cut(s) 246
BpuEI CTTGAG 1 cut(s) 334
BsaJI CCNNGG 4 cut(s) 97, 106, 207, 522
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse1I ACTGG 3 cut(s) 330, 398, 449
Bse3DI GCAATG 1 cut(s) 87
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 4 cut(s) 97, 106, 207, 522
BseGI GGATG 1 cut(s) 85
BseLI CCNNNNNNNGG 1 cut(s) 269
BseMI GCAATG 1 cut(s) 87
BseNI ACTGG 3 cut(s) 330, 398, 449
BseSI GKGCMC 1 cut(s) 160
BseYI CCCAGC 1 cut(s) 405
BsgI GTGCAG 1 cut(s) 109
BshFI GGCC 2 cut(s) 329, 438
BsiHKAI GWGCWC 2 cut(s) 160, 350
BslI CCNNNNNNNGG 1 cut(s) 269
BsmAI GTCTC 1 cut(s) 140
BsnI GGCC 2 cut(s) 329, 438
Bsp1286I GDGCHC 2 cut(s) 160, 350
Bsp143I GATC 4 cut(s) 214, 219, 241, 383
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 1 cut(s) 294
BspANI GGCC 2 cut(s) 329, 438
BspLI GGNNCC 1 cut(s) 112
BspPI GGATC 2 cut(s) 236, 391
BsrDI GCAATG 1 cut(s) 87
BsrI ACTGG 3 cut(s) 330, 398, 449
BssECI CCNNGG 4 cut(s) 97, 106, 207, 522
BssMI GATC 4 cut(s) 214, 219, 241, 383
BssT1I CCWWGG 4 cut(s) 97, 106, 207, 522
Bst2UI CCWGG 1 cut(s) 402
BstAPI GCANNNNNTGC 1 cut(s) 164
BstDEI CTNAG 1 cut(s) 246
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 1 cut(s) 85
BstKTI GATC 4 cut(s) 217, 222, 244, 386
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 4 cut(s) 214, 219, 241, 383
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNI CCWGG 1 cut(s) 402
BstSCI CCNGG 1 cut(s) 400
BstSLI GKGCMC 1 cut(s) 160
BstV2I GAAGAC 2 cut(s) 26, 46
BsuRI GGCC 2 cut(s) 329, 438
BtgI CCRYGG 1 cut(s) 97
BtrI CACGTC 1 cut(s) 319
BtsCI GGATG 1 cut(s) 85
BtsIMutI CAGTG 1 cut(s) 337
Cfr13I GGNCC 1 cut(s) 335
CseI GACGC 1 cut(s) 178
Csp6I GTAC 3 cut(s) 367, 446, 536
CviAII CATG 7 cut(s) 98, 182, 281, 339, 343, 440, 542
CviJI RGCY 9 cut(s) 10, 102, 113, 250, 329, 348, 405, 438, 504
CviKI_1 RGCY 9 cut(s) 10, 102, 113, 250, 329, 348, 405, 438, 504
CviQI GTAC 3 cut(s) 367, 446, 536
DdeI CTNAG 1 cut(s) 246
DpnI GATC 4 cut(s) 216, 221, 243, 385
DpnII GATC 4 cut(s) 214, 219, 241, 383
EaeI YGGCCR 1 cut(s) 436
Ecl136II GAGCTC 1 cut(s) 348
Eco130I CCWWGG 4 cut(s) 97, 106, 207, 522
Eco24I GRGCYC 1 cut(s) 350
Eco47I GGWCC 1 cut(s) 335
Eco53kI GAGCTC 1 cut(s) 348
Eco57I CTGAAG 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 348
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 4 cut(s) 97, 106, 207, 522
EcoT22I ATGCAT 2 cut(s) 87, 344
EcoT38I GRGCYC 1 cut(s) 350
ErhI CCWWGG 4 cut(s) 97, 106, 207, 522
FaeI CATG 7 cut(s) 101, 185, 284, 342, 346, 443, 545
FatI CATG 7 cut(s) 97, 181, 280, 338, 342, 439, 541
FauI CCCGC 1 cut(s) 287
FbaI TGATCA 1 cut(s) 219
FokI GGATG 1 cut(s) 72
FriOI GRGCYC 1 cut(s) 350
FspBI CTAG 2 cut(s) 50, 562
GsaI CCCAGC 1 cut(s) 409
HaeIII GGCC 2 cut(s) 329, 438
HgaI GACGC 1 cut(s) 178
Hin1II CATG 7 cut(s) 101, 185, 284, 342, 346, 443, 545
HindIII AAGCTT 1 cut(s) 8
HinfI GANTC 2 cut(s) 195, 203
HphI GGTGA 2 cut(s) 224, 421
Hpy166II GTNNAC 2 cut(s) 158, 335
Hpy188I TCNGA 6 cut(s) 194, 219, 237, 264, 383, 388
Hpy188III TCNNGA 1 cut(s) 485
Hpy8I GTNNAC 2 cut(s) 158, 335
HpyAV CCTTC 1 cut(s) 406
HpyCH4IV ACGT 2 cut(s) 318, 492
HpyCH4V TGCA 6 cut(s) 70, 80, 85, 126, 158, 342
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 1 cut(s) 246
HpySE526I ACGT 2 cut(s) 318, 492
Hsp92II CATG 7 cut(s) 101, 185, 284, 342, 346, 443, 545
Ksp22I TGATCA 1 cut(s) 219
Kzo9I GATC 4 cut(s) 214, 219, 241, 383
LmnI GCTCC 2 cut(s) 110, 265
LpnPI CCDG 9 cut(s) 166, 343, 379, 383, 387, 391, 414, 462, 498
LweI GCATC 2 cut(s) 94, 241
MaeI CTAG 2 cut(s) 50, 562
MaeII ACGT 2 cut(s) 318, 492
MaeIII GTNAC 1 cut(s) 515
MalI GATC 4 cut(s) 216, 221, 243, 385
MboI GATC 4 cut(s) 214, 219, 241, 383
MboII GAAGA 6 cut(s) 31, 36, 46, 49, 315, 385
MhlI GDGCHC 2 cut(s) 160, 350
MlsI TGGCCA 1 cut(s) 438
MluNI TGGCCA 1 cut(s) 438
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 1 cut(s) 361
MnlI CCTC 3 cut(s) 278, 319, 549
Mox20I TGGCCA 1 cut(s) 438
Mph1103I ATGCAT 2 cut(s) 87, 344
MscI TGGCCA 1 cut(s) 438
MslI CAYNNNNRTG 1 cut(s) 317
Msp20I TGGCCA 1 cut(s) 438
MspR9I CCNGG 1 cut(s) 402
MvaI CCWGG 1 cut(s) 402
MwoI GCNNNNNNNGC 1 cut(s) 164
NcoI CCATGG 1 cut(s) 97
NdeII GATC 4 cut(s) 214, 219, 241, 383
NlaIII CATG 7 cut(s) 101, 185, 284, 342, 346, 443, 545
NlaIV GGNNCC 1 cut(s) 112
NmuCI GTSAC 1 cut(s) 515
NsiI ATGCAT 2 cut(s) 87, 344
PfeI GAWTC 1 cut(s) 195
PflFI GACNNNGTC 1 cut(s) 182
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
Psp124BI GAGCTC 1 cut(s) 350
Psp1406I AACGTT 1 cut(s) 492
Psp6I CCWGG 1 cut(s) 400
PspFI CCCAGC 1 cut(s) 405
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 1 cut(s) 112
PspPI GGNCC 1 cut(s) 335
PsyI GACNNNGTC 1 cut(s) 182
RsaI GTAC 3 cut(s) 368, 447, 537
RsaNI GTAC 3 cut(s) 367, 446, 536
RseI CAYNNNNRTG 1 cut(s) 317
SacI GAGCTC 1 cut(s) 350
Sau3AI GATC 4 cut(s) 214, 219, 241, 383
Sau96I GGNCC 1 cut(s) 335
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 402
SduI GDGCHC 2 cut(s) 160, 350
SfaNI GCATC 2 cut(s) 94, 241
SinI GGWCC 1 cut(s) 335
SmiMI CAYNNNNRTG 1 cut(s) 317
SmlI CTYRAG 1 cut(s) 349
SmoI CTYRAG 1 cut(s) 349
SpeI ACTAGT 1 cut(s) 49
SsiI CCGC 1 cut(s) 294
SspMI CTAG 2 cut(s) 50, 562
SstI GAGCTC 1 cut(s) 350
StyD4I CCNGG 1 cut(s) 400
StyI CCWWGG 4 cut(s) 97, 106, 207, 522
TaiI ACGT 2 cut(s) 321, 495
TaqI TCGA 1 cut(s) 424
TfiI GAWTC 1 cut(s) 195
TscAI CASTG 1 cut(s) 337
TseFI GTSAC 1 cut(s) 515
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 3 cut(s) 32, 49, 456
TspRI CASTG 1 cut(s) 337
Tth111I GACNNNGTC 1 cut(s) 182
VneI GTGCAC 1 cut(s) 156
VpaK11BI GGWCC 1 cut(s) 335
XcmI CCANNNNNNNNNTGG 1 cut(s) 492
XspI CTAG 2 cut(s) 50, 562
Zsp2I ATGCAT 2 cut(s) 87, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.