pycom17g06770

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001586
Physical Location & Seq
Forward (+)
4905082 .. 4905573
492 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g06770.1

Sequence Viewer

Length: 492 bp
ATGGCCCCAAGACCAAGAGGACGACCCAGGAAACGCAATACTCGAATGTGTGCTGCGATTGATGCTATGAAACCCATGGGTTTTTCGGAGAATTTAGTTTGCGATACAGTGAAAGAACTACTGGAAGTTTATGGGGAAGATGGTGATACAACTGGATGGCCCTTTATCGAAGAAGGTGCTTACTCCCTCCTGATTGAAACAATTCTTGAGAAACAAGGCAGTGCTGAAAACAAGGATGCTTCTCTTCTAGATGATGCAGGAAGTGGAAATGGCGTCCAAGTGTCATCAGCTAAGCCCTCAATCACTGTTTTTTGTCCGGATAGTGTTGCACCGCGGAGGCTTAGACCTTTGTACGGCTGGATTTGTAGGGTTGATGATGATGATAATAAGCTACCTGTGGAACTAGCACCGGTTCCGTCATTGGCGTTTGTCGCCGCTTTATTGCGCAGATGGGAGAAGAAGGTGCAAGTCAAGGTGGAATGCGAGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WIYLD

Protein Analysis

164

Amino Acids

17.97

Weight (kDa)

5.16

Isoelectric Point (pI)

51.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WIYLD PF10440 10 - 72 3e-20 Ubiquitin-binding WIYLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 446
AccII CGCG 1 cut(s) 334
AccIII TCCGGA 1 cut(s) 316
AciI CCGC 3 cut(s) 332, 334, 435
AcsI RAATTY 1 cut(s) 91
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 1 cut(s) 353
AfiI CCNNNNNNNGG 1 cut(s) 353
AgeI ACCGGT 1 cut(s) 409
AgsI TTSAA 1 cut(s) 197
AjnI CCWGG 1 cut(s) 26
AluBI AGCT 2 cut(s) 290, 391
AluI AGCT 2 cut(s) 290, 391
Aor13HI TCCGGA 1 cut(s) 316
AoxI GGCC 3 cut(s) 3, 158, 486
ApeKI GCWGC 1 cut(s) 53
ApoI RAATTY 1 cut(s) 91
AsiGI ACCGGT 1 cut(s) 409
Asp700I GAANNNNTTC 1 cut(s) 201
AspLEI GCGC 1 cut(s) 447
AspS9I GGNCC 2 cut(s) 4, 159
AsuHPI GGTGA 1 cut(s) 155
BbvI GCAGC 1 cut(s) 40
BccI CCATC 3 cut(s) 134, 150, 444
BceAI ACGGC 1 cut(s) 370
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BciT130I CCWGG 1 cut(s) 28
BfaI CTAG 2 cut(s) 248, 404
BisI GCNGC 2 cut(s) 54, 435
BlpI GCTNAGC 1 cut(s) 291
BlsI GCNGC 2 cut(s) 55, 436
Bme1390I CCNGG 1 cut(s) 28
BmgT120I GGNCC 2 cut(s) 4, 159
BmiI GGNNCC 2 cut(s) 6, 414
BmrFI CCNGG 1 cut(s) 28
BmsI GCATC 3 cut(s) 52, 226, 244
Bpu1102I GCTNAGC 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 227
BsaHI GRCGYC 1 cut(s) 273
BsaJI CCNNGG 3 cut(s) 26, 75, 332
BsaWI WCCGGW 2 cut(s) 316, 409
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse118I RCCGGY 1 cut(s) 409
Bse1I ACTGG 2 cut(s) 126, 157
BseAI TCCGGA 1 cut(s) 316
BseBI CCWGG 1 cut(s) 28
BseDI CCNNGG 3 cut(s) 26, 75, 332
BseGI GGATG 2 cut(s) 161, 241
BseLI CCNNNNNNNGG 1 cut(s) 353
BseNI ACTGG 2 cut(s) 126, 157
BseXI GCAGC 1 cut(s) 40
Bsh1236I CGCG 1 cut(s) 334
BshFI GGCC 3 cut(s) 5, 160, 488
BshTI ACCGGT 1 cut(s) 409
BsiSI CCGG 2 cut(s) 317, 410
BslI CCNNNNNNNGG 1 cut(s) 353
BsmI GAATGC 1 cut(s) 485
BsnI GGCC 3 cut(s) 5, 160, 488
Bsp13I TCCGGA 1 cut(s) 316
Bsp1720I GCTNAGC 1 cut(s) 291
Bsp19I CCATGG 1 cut(s) 75
BspACI CCGC 3 cut(s) 332, 334, 435
BspANI GGCC 3 cut(s) 5, 160, 488
BspEI TCCGGA 1 cut(s) 316
BspFNI CGCG 1 cut(s) 334
BspLI GGNNCC 2 cut(s) 6, 414
BsrFI RCCGGY 1 cut(s) 409
BsrI ACTGG 2 cut(s) 126, 157
BssAI RCCGGY 1 cut(s) 409
BssECI CCNNGG 3 cut(s) 26, 75, 332
BssNI GRCGYC 1 cut(s) 273
BssT1I CCWWGG 1 cut(s) 75
Bst2UI CCWGG 1 cut(s) 28
Bst4CI ACNGT 2 cut(s) 109, 307
Bst6I CTCTTC 1 cut(s) 249
BstACI GRCGYC 1 cut(s) 273
BstDEI CTNAG 2 cut(s) 291, 341
BstDSI CCRYGG 2 cut(s) 75, 332
BstF5I GGATG 2 cut(s) 161, 241
BstFNI CGCG 1 cut(s) 334
BstHHI GCGC 1 cut(s) 447
BstMWI GCNNNNNNNGC 2 cut(s) 62, 431
BstNI CCWGG 1 cut(s) 28
BstSCI CCNGG 1 cut(s) 26
BstUI CGCG 1 cut(s) 334
BstV1I GCAGC 1 cut(s) 40
BsuRI GGCC 3 cut(s) 5, 160, 488
BtgI CCRYGG 2 cut(s) 75, 332
BtsCI GGATG 2 cut(s) 161, 241
BtsI GCAGTG 1 cut(s) 226
BtsIMutI CAGTG 3 cut(s) 114, 226, 303
CfoI GCGC 1 cut(s) 447
Cfr10I RCCGGY 1 cut(s) 409
Cfr13I GGNCC 2 cut(s) 4, 159
Cfr42I CCGCGG 1 cut(s) 335
CseI GACGC 1 cut(s) 262
Csp6I GTAC 1 cut(s) 352
CspAI ACCGGT 1 cut(s) 409
CviAII CATG 1 cut(s) 76
CviJI RGCY 8 cut(s) 5, 160, 290, 295, 340, 357, 391, 488
CviKI_1 RGCY 8 cut(s) 5, 160, 290, 295, 340, 357, 391, 488
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 2 cut(s) 291, 341
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 75
Eco147I AGGCCT 1 cut(s) 488
EcoRII CCWGG 1 cut(s) 26
EcoT14I CCWWGG 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 1 cut(s) 79
FaiI YATR 3 cut(s) 68, 77, 132
FatI CATG 1 cut(s) 75
Fnu4HI GCNGC 2 cut(s) 54, 435
FokI GGATG 2 cut(s) 168, 248
Fsp4HI GCNGC 2 cut(s) 54, 435
FspBI CTAG 2 cut(s) 248, 404
FspI TGCGCA 1 cut(s) 446
GlaI GCGC 1 cut(s) 446
GluI GCNGC 2 cut(s) 54, 435
HaeIII GGCC 3 cut(s) 5, 160, 488
HapII CCGG 2 cut(s) 317, 410
HgaI GACGC 1 cut(s) 262
HhaI GCGC 1 cut(s) 447
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 1 cut(s) 79
Hin6I GCGC 1 cut(s) 445
HinP1I GCGC 1 cut(s) 445
HpaII CCGG 2 cut(s) 317, 410
HphI GGTGA 1 cut(s) 155
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 4 cut(s) 190, 206, 248, 317
HpyAV CCTTC 2 cut(s) 167, 454
HpyCH4III ACNGT 2 cut(s) 109, 307
HpyCH4V TGCA 3 cut(s) 257, 329, 466
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 431
HpyF3I CTNAG 2 cut(s) 291, 341
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 1 cut(s) 79
HspAI GCGC 1 cut(s) 445
Kpn2I TCCGGA 1 cut(s) 316
KspI CCGCGG 1 cut(s) 335
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 3 cut(s) 52, 226, 244
MaeI CTAG 2 cut(s) 248, 404
MboII GAAGA 4 cut(s) 149, 182, 236, 469
MluCI AATT 2 cut(s) 91, 201
MnlI CCTC 5 cut(s) 11, 197, 307, 330, 478
MroI TCCGGA 1 cut(s) 316
MroXI GAANNNNTTC 1 cut(s) 201
MspA1I CMGCKG 1 cut(s) 334
MspI CCGG 2 cut(s) 317, 410
MspR9I CCNGG 1 cut(s) 28
Mva1269I GAATGC 1 cut(s) 485
MvaI CCWGG 1 cut(s) 28
MvnI CGCG 1 cut(s) 334
MwoI GCNNNNNNNGC 2 cut(s) 62, 431
NcoI CCATGG 1 cut(s) 75
NlaIII CATG 1 cut(s) 79
NlaIV GGNNCC 2 cut(s) 6, 414
NsbI TGCGCA 1 cut(s) 446
PceI AGGCCT 1 cut(s) 488
PcsI WCGNNNNNNNCGW 1 cut(s) 40
PctI GAATGC 1 cut(s) 485
PdmI GAANNNNTTC 1 cut(s) 201
PinAI ACCGGT 1 cut(s) 409
PkrI GCNGC 2 cut(s) 55, 436
Psp6I CCWGG 1 cut(s) 26
PspGI CCWGG 1 cut(s) 26
PspN4I GGNNCC 2 cut(s) 6, 414
PspPI GGNCC 2 cut(s) 4, 159
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
SacII CCGCGG 1 cut(s) 335
SatI GCNGC 2 cut(s) 54, 435
Sau96I GGNCC 2 cut(s) 4, 159
ScrFI CCNGG 1 cut(s) 28
SetI ASST 7 cut(s) 178, 292, 349, 393, 397, 465, 477
SfaNI GCATC 3 cut(s) 52, 226, 244
Sfr303I CCGCGG 1 cut(s) 335
SgrBI CCGCGG 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 2 cut(s) 91, 201
SseBI AGGCCT 1 cut(s) 488
SsiI CCGC 3 cut(s) 332, 334, 435
SspMI CTAG 2 cut(s) 248, 404
StuI AGGCCT 1 cut(s) 488
StyD4I CCNGG 1 cut(s) 26
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 2 cut(s) 109, 307
TaqI TCGA 2 cut(s) 43, 168
TasI AATT 2 cut(s) 91, 201
TauI GCSGC 1 cut(s) 437
TscAI CASTG 3 cut(s) 114, 226, 310
TseI GCWGC 1 cut(s) 53
TspDTI ATGAA 1 cut(s) 83
TspGWI ACGGA 1 cut(s) 405
TspRI CASTG 3 cut(s) 114, 226, 310
XapI RAATTY 1 cut(s) 91
XbaI TCTAGA 1 cut(s) 247
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 2 cut(s) 248, 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.