Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0002292

Physical Location & Seq

Genomic Coordinates Forward (+)

27653814 .. 27656413

Gene Structure 2600 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1175400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1347 bp

ATGGATTTCAGACTGTTTGAGGCTTCTCAAACAGGAGATAACCAGCTCTTGCACCAGTTGCTAGCAGAGAATCCACTTCTACTCCATGACCTTGCCCTAACTTCCACCGAGAACCCGCTACATGTCGCTTCGTTTGCCGGACATGTCGAATTCGTTAAGGAGATTGTGAAGTTGAAACCAGCTTTTGTGTTAGAAATGAACCAGGATGGGTTTGGCCCCATGCACATCGCGTCAGCCAACGGTTACTTGGAGATTGTAAGGGAGCTGCTGAGAGTTGACCCGAGACTCTGCCGGATGAACGGAAGAGATGGATGGACTCCTCTTCATTGTGCTGCTGCTAAGGGAAGGGCTGATGTTGTCGGAGAAATGGTTTTGACCTGCCCGGAAAGTGTCGAAGATGTGACTCTACAAGGGGAGACTTCCTTGCATCTTGCTGTTAAGAACTGCCAGTTTGAAGCAGTTAAACTGTTGGTTGAATTGGTCAGGGAACTGAGGAAGGTGAACGTTTTGAATATGAAGGACAAACATGGTAATTCAGTTCTTCACCTAGCAACCTGGAAGAAACTGCACGAGGTTGTGGAGTGGTTAGTTGGCAATGGAACTACTCCAAGCGCATTGGAAGTAAACATTGTAAACCAGGGTGGTCTCACACCTCTTGATCTTCTACTAGTCTTCCCGAGTGAAGCCGGTGATAGGGAAATCGAAGAGATCCTTCGTAGTGCCGGAGCTTCGAGAGCACAAGACATAAACCATACTCCCTCTCATTGCCCCATGGAAACCAATACCCTTCAGCTGCAGCAGCCAAAAAACTTGATGGAATACTTCAAGTTCAAAAAGGGCAGAGACTCTCCTAGTGATGCCCGCACTGCGCTACTAGTTATCTCTGTACTAGTGGCTACCGCCACCTTTCAAGTTGGACTCAGCCCTCCGAATGGCATTTGGCAGGACAGCTCCGGATCAAACAATAATGGAGCTGGCAGTGGGAGCAATGAACCAGCTGCACACTTAGCAGGGAAGTCCATCATGGGTTCTAACAATGCAGTTGCATTTGTCTTCTTTGTGGCTTTCAACTCGATTGGATTTTCACTTTCACTTTACATGATCAATGTCCTCACCAGCAACTTCCCTCTGCAGTTGGAGCTTCAAATCTGTATCGTTGCGGTCTACTGCACGTACAACTTTGCAGTTACCAGCATTGCACCGGATAACACGAAGCTCATCATAATTGTGGTCACTTCAGTTTTGCCTACTTTGGTTTCACTTGCTGTCCCATGCGCTAGACAGCTTATCATGATCAGGGTTAGAGAACCTGTGCTAAATTGGACTCGAAGGTTCATTTTACAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Ank_4 PGG

Protein Analysis

449

Amino Acids

49.16

Weight (kDa)

6.02

Isoelectric Point (pI)

29.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	5 - 91	2.8e-10	Ankyrin repeats (3 copies)
Ank_2	PF12796	99 - 159	2.5e-09	Ankyrin repeats (3 copies)
Ank_2	PF12796	135 - 202	3.8e-09	Ankyrin repeats (3 copies)
PGG	PF13962	286 - 390	2.1e-14	Domain of unknown function

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002292)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51160
fragaria_vesca	FvH4_7g26641 FvH4_7g26643 FvH4_7g26670
malus_domestica	MD01G1175400.v1.1 MD07G1241300.v1.1 MD07G1241500.v1.1 MD12G1230800.v1.1 MD12G1230900.v1.1 MD12G1231100.v1.1 MD12G1231200.v1.1
prunus_persica	Prupe.2G270400_v2.0.a1 Prupe.2G270500_v2.0.a1
pyrus_communis	pycom07g21780 pycom07g21790 pycom07g21800 pycom07g21860 pycom07g21970 pycom12g21410
rosa_chinensis	RchiOBHm_Chr1g0373271 RchiOBHm_Chr1g0373281
rosa_laevigata	RLG00000026830 RLG00000026831 RLG00000026832
rosa_multiflora	Rmu_co8287043.1_g000001 Rmu_sc0000170.1_g000005 Rmu_ssc0000435.1_g000001 Rmu_ssc0000435.1_g000002
rosa_rugosa	Rorug01G0376800 Rorug01G0376800
rosa_samantha	Rh1AG385600 Rh1AG385700 Rh1BG349800 Rh1BG349900 Rh1CG363000 Rh1CG363100 Rh1DG380500 Rh1DG380600 Rh1DG380700 Rh7DG228200
rosa_wichuraiana	Rw1G034920 Rw1G034930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	386
AccI	GTMKAC	1 cut(s)	1164
AccII	CGCG	1 cut(s)	230
AccIII	TCCGGA	1 cut(s)	953
AciI	CCGC	4 cut(s)	116, 862, 900, 1160
AclI	AACGTT	1 cut(s)	504
AclWI	GGATC	2 cut(s)	703, 964
AcsI	RAATTY	1 cut(s)	149
AcuI	CTGAAG	2 cut(s)	773, 1221
AfaI	GTAC	2 cut(s)	888, 1175
AfiI	CCNNNNNNNGG	2 cut(s)	693, 932
AflIII	ACRYGT	2 cut(s)	121, 142
AgsI	TTSAA	9 cut(s)	175, 455, 476, 511, 826, 832, 911, 1069, 1145
AhlI	ACTAGT	3 cut(s)	667, 874, 889
AjnI	CCWGG	3 cut(s)	201, 554, 636
Alw21I	GWGCWC	1 cut(s)	739
Alw26I	GTCTC	4 cut(s)	277, 410, 650, 837
AlwI	GGATC	2 cut(s)	703, 964
Ama87I	CYCGRG	2 cut(s)	280, 676
Aor13HI	TCCGGA	1 cut(s)	953
AoxI	GGCC	1 cut(s)	214
ApeKI	GCWGC	7 cut(s)	265, 332, 335, 793, 796, 799, 998
ApoI	RAATTY	1 cut(s)	149
AspLEI	GCGC	3 cut(s)	614, 871, 1277
AspS9I	GGNCC	1 cut(s)	215
AsuC2I	CCSGG	1 cut(s)	383
AsuHPI	GGTGA	4 cut(s)	511, 536, 701, 1105
AsuNHI	GCTAGC	1 cut(s)	61
AvaI	CYCGRG	2 cut(s)	280, 676
BauI	CACGAG	1 cut(s)	569
BbsI	GAAGAC	2 cut(s)	664, 1045
Bbv12I	GWGCWC	1 cut(s)	739
BbvI	GCAGC	7 cut(s)	252, 319, 322, 780, 808, 811, 985
BccI	CCATC	5 cut(s)	200, 302, 306, 808, 1028
BciT130I	CCWGG	3 cut(s)	203, 556, 638
BclI	TGATCA	2 cut(s)	1101, 1293
BcnI	CCSGG	1 cut(s)	383
BcoDI	GTCTC	4 cut(s)	277, 410, 650, 837
BcuI	ACTAGT	3 cut(s)	667, 874, 889
BfaI	CTAG	7 cut(s)	62, 548, 668, 852, 875, 890, 1278
BfmI	CTRYAG	2 cut(s)	794, 1130
BfuAI	ACCTGC	1 cut(s)	386
BisI	GCNGC	7 cut(s)	266, 333, 336, 794, 797, 800, 999
BlsI	GCNGC	7 cut(s)	267, 334, 337, 795, 798, 801, 1000
Bme1390I	CCNGG	4 cut(s)	203, 383, 556, 638
BmeT110I	CYCGRG	2 cut(s)	280, 676
BmgT120I	GGNCC	1 cut(s)	215
BmiI	GGNNCC	1 cut(s)	217
BmrFI	CCNGG	4 cut(s)	203, 383, 556, 638
BmsI	GCATC	2 cut(s)	436, 847
BmtI	GCTAGC	1 cut(s)	65
BpiI	GAAGAC	2 cut(s)	664, 1045
Bpu10I	CCTNAGC	1 cut(s)	339
BpuMI	CCSGG	1 cut(s)	383
BsaAI	YACGTR	1 cut(s)	1173
BsaI	GGTCTC	1 cut(s)	650
BsaJI	CCNNGG	2 cut(s)	637, 771
BsaWI	WCCGGW	2 cut(s)	953, 1201
BsaXI	ACNNNNNCTCC	4 cut(s)	66, 96, 354, 384
Bsc4I	CCNNNNNNNGG	2 cut(s)	693, 932
Bse118I	RCCGGY	1 cut(s)	686
Bse1I	ACTGG	2 cut(s)	55, 448
Bse3DI	GCAATG	4 cut(s)	601, 763, 994, 1194
BseAI	TCCGGA	1 cut(s)	953
BseBI	CCWGG	3 cut(s)	203, 556, 638
BseDI	CCNNGG	2 cut(s)	637, 771
BseGI	GGATG	3 cut(s)	211, 300, 317
BseLI	CCNNNNNNNGG	2 cut(s)	693, 932
BseMI	GCAATG	4 cut(s)	601, 763, 994, 1194
BseMII	CTCAG	3 cut(s)	260, 482, 934
BseNI	ACTGG	2 cut(s)	55, 448
BseRI	GAGGAG	1 cut(s)	309
BseXI	GCAGC	7 cut(s)	252, 319, 322, 780, 808, 811, 985
BsgI	GTGCAG	3 cut(s)	551, 984, 1153
Bsh1236I	CGCG	1 cut(s)	230
BshFI	GGCC	1 cut(s)	216
BsiHKAI	GWGCWC	1 cut(s)	739
BsiHKCI	CYCGRG	2 cut(s)	280, 676
BsiSI	CCGG	7 cut(s)	138, 292, 383, 687, 723, 954, 1202
BslFI	GGGAC	1 cut(s)	1253
BslI	CCNNNNNNNGG	2 cut(s)	693, 932
BsmAI	GTCTC	4 cut(s)	277, 410, 650, 837
BsmFI	GGGAC	1 cut(s)	1253
BsnI	GGCC	1 cut(s)	216
Bso31I	GGTCTC	1 cut(s)	650
BsoBI	CYCGRG	2 cut(s)	280, 676
Bsp1286I	GDGCHC	1 cut(s)	739
Bsp13I	TCCGGA	1 cut(s)	953
Bsp143I	GATC	5 cut(s)	658, 708, 956, 1101, 1293
Bsp19I	CCATGG	1 cut(s)	771
BspACI	CCGC	4 cut(s)	116, 862, 900, 1160
BspANI	GGCC	1 cut(s)	216
BspCNI	CTCAG	3 cut(s)	261, 483, 933
BspEI	TCCGGA	1 cut(s)	953
BspFNI	CGCG	1 cut(s)	230
BspHI	TCATGA	1 cut(s)	1290
BspLI	GGNNCC	1 cut(s)	217
BspMAI	CTGCAG	2 cut(s)	798, 1134
BspMI	ACCTGC	1 cut(s)	386
BspOI	GCTAGC	1 cut(s)	65
BspPI	GGATC	2 cut(s)	703, 964
BspTNI	GGTCTC	1 cut(s)	650
BsrDI	GCAATG	4 cut(s)	601, 763, 994, 1194
BsrFI	RCCGGY	1 cut(s)	686
BsrI	ACTGG	2 cut(s)	55, 448
BssAI	RCCGGY	1 cut(s)	686
BssECI	CCNNGG	2 cut(s)	637, 771
BssMI	GATC	5 cut(s)	658, 708, 956, 1101, 1293
BssSI	CACGAG	1 cut(s)	569
BssT1I	CCWWGG	1 cut(s)	771
Bst2BI	CACGAG	1 cut(s)	569
Bst2UI	CCWGG	3 cut(s)	203, 556, 638
Bst4CI	ACNGT	3 cut(s)	15, 242, 468
Bst6I	CTCTTC	3 cut(s)	298, 327, 699
BstAPI	GCANNNNNTGC	1 cut(s)	58
BstBAI	YACGTR	1 cut(s)	1173
BstC8I	GCNNGC	3 cut(s)	63, 862, 976
BstDEI	CTNAG	5 cut(s)	269, 339, 491, 920, 1006
BstDSI	CCRYGG	1 cut(s)	771
BstF5I	GGATG	3 cut(s)	211, 300, 317
BstFNI	CGCG	1 cut(s)	230
BstHHI	GCGC	3 cut(s)	614, 871, 1277
BstKTI	GATC	5 cut(s)	661, 711, 959, 1104, 1296
BstMAI	GTCTC	4 cut(s)	277, 410, 650, 837
BstMBI	GATC	5 cut(s)	658, 708, 956, 1101, 1293
BstMWI	GCNNNNNNNGC	8 cut(s)	58, 134, 734, 799, 866, 984, 1007, 1138
BstNI	CCWGG	3 cut(s)	203, 556, 638
BstNSI	RCATGY	2 cut(s)	125, 146
BstSCI	CCNGG	4 cut(s)	201, 381, 554, 636
BstSFI	CTRYAG	2 cut(s)	794, 1130
BstUI	CGCG	1 cut(s)	230
BstV1I	GCAGC	7 cut(s)	252, 319, 322, 780, 808, 811, 985
BstV2I	GAAGAC	2 cut(s)	664, 1045
BstX2I	RGATCY	1 cut(s)	708
BstYI	RGATCY	1 cut(s)	708
BsuRI	GGCC	1 cut(s)	216
BtgI	CCRYGG	1 cut(s)	771
BtgZI	GCGATG	1 cut(s)	211
BtsCI	GGATG	3 cut(s)	211, 300, 317
BtsI	GCAGTG	2 cut(s)	864, 985
BtsIMutI	CAGTG	2 cut(s)	864, 985
BveI	ACCTGC	1 cut(s)	386
Cac8I	GCNNGC	3 cut(s)	63, 862, 976
CciI	TCATGA	1 cut(s)	1290
CfoI	GCGC	3 cut(s)	614, 871, 1277
Cfr10I	RCCGGY	1 cut(s)	686
Cfr13I	GGNCC	1 cut(s)	215
CseI	GACGC	1 cut(s)	219
Csp6I	GTAC	2 cut(s)	887, 1174
CviQI	GTAC	2 cut(s)	887, 1174
DdeI	CTNAG	5 cut(s)	269, 339, 491, 920, 1006
DpnI	GATC	5 cut(s)	660, 710, 958, 1103, 1295
DpnII	GATC	5 cut(s)	658, 708, 956, 1101, 1293
Eam1104I	CTCTTC	3 cut(s)	298, 327, 699
EarI	CTCTTC	3 cut(s)	298, 327, 699
Eco130I	CCWWGG	1 cut(s)	771
Eco31I	GGTCTC	1 cut(s)	650
Eco57I	CTGAAG	2 cut(s)	773, 1221
Eco88I	CYCGRG	2 cut(s)	280, 676
EcoRI	GAATTC	1 cut(s)	149
EcoRII	CCWGG	3 cut(s)	201, 554, 636
EcoT14I	CCWWGG	1 cut(s)	771
ErhI	CCWWGG	1 cut(s)	771
FalI	AAGNNNNNCTT	2 cut(s)	696, 728
FaqI	GGGAC	1 cut(s)	1253
FauI	CCCGC	2 cut(s)	123, 869
FbaI	TGATCA	2 cut(s)	1101, 1293
FblI	GTMKAC	1 cut(s)	1164
Fnu4HI	GCNGC	7 cut(s)	266, 333, 336, 794, 797, 800, 999
FokI	GGATG	3 cut(s)	218, 307, 324
Fsp4HI	GCNGC	7 cut(s)	266, 333, 336, 794, 797, 800, 999
FspBI	CTAG	7 cut(s)	62, 548, 668, 852, 875, 890, 1278
GlaI	GCGC	3 cut(s)	613, 870, 1276
GluI	GCNGC	7 cut(s)	266, 333, 336, 794, 797, 800, 999
HaeIII	GGCC	1 cut(s)	216
HapII	CCGG	7 cut(s)	138, 292, 383, 687, 723, 954, 1202
HgaI	GACGC	1 cut(s)	219
HhaI	GCGC	3 cut(s)	614, 871, 1277
Hin6I	GCGC	3 cut(s)	612, 869, 1275
HinP1I	GCGC	3 cut(s)	612, 869, 1275
HincII	GTYRAC	1 cut(s)	277
HindII	GTYRAC	1 cut(s)	277
HinfI	GANTC	7 cut(s)	70, 285, 316, 403, 845, 918, 1324
HpaII	CCGG	7 cut(s)	138, 292, 383, 687, 723, 954, 1202
HphI	GGTGA	4 cut(s)	511, 536, 701, 1105
Hpy166II	GTNNAC	5 cut(s)	277, 502, 625, 634, 1165
Hpy188I	TCNGA	3 cut(s)	11, 362, 930
Hpy188III	TCNNGA	5 cut(s)	656, 676, 732, 954, 1291
Hpy8I	GTNNAC	5 cut(s)	277, 502, 625, 634, 1165
HpyAV	CCTTC	6 cut(s)	339, 490, 511, 722, 797, 1323
HpyCH4III	ACNGT	3 cut(s)	15, 242, 468
HpyCH4IV	ACGT	2 cut(s)	504, 1172
HpyF10VI	GCNNNNNNNGC	8 cut(s)	58, 134, 734, 799, 866, 984, 1007, 1138
HpyF3I	CTNAG	5 cut(s)	269, 339, 491, 920, 1006
HpySE526I	ACGT	2 cut(s)	504, 1172
HspAI	GCGC	3 cut(s)	612, 869, 1275
Kpn2I	TCCGGA	1 cut(s)	953
Ksp22I	TGATCA	2 cut(s)	1101, 1293
Kzo9I	GATC	5 cut(s)	658, 708, 956, 1101, 1293
LmnI	GCTCC	6 cut(s)	262, 725, 956, 971, 984, 1138
Lsp1109I	GCAGC	7 cut(s)	252, 319, 322, 780, 808, 811, 985
LweI	GCATC	2 cut(s)	436, 847
MaeI	CTAG	7 cut(s)	62, 548, 668, 852, 875, 890, 1278
MaeII	ACGT	2 cut(s)	504, 1172
MaeIII	GTNAC	4 cut(s)	242, 400, 1186, 1231
MalI	GATC	5 cut(s)	660, 710, 958, 1103, 1295
MboI	GATC	5 cut(s)	658, 708, 956, 1101, 1293
MboII	GAAGA	9 cut(s)	314, 315, 407, 533, 571, 653, 664, 716, 1045
MflI	RGATCY	1 cut(s)	708
MhlI	GDGCHC	1 cut(s)	739
MluCI	AATT	5 cut(s)	149, 476, 532, 1224, 1318
MlyI	GAGTC	6 cut(s)	279, 310, 397, 839, 912, 1318
MmeI	TCCRAC	3 cut(s)	340, 895, 1116
MnlI	CCTC	9 cut(s)	13, 330, 486, 565, 663, 769, 936, 1121, 1137
MroI	TCCGGA	1 cut(s)	953
MseI	TTAA	3 cut(s)	156, 438, 462
MslI	CAYNNNNRTG	1 cut(s)	1226
MspA1I	CMGCKG	2 cut(s)	793, 998
MspI	CCGG	7 cut(s)	138, 292, 383, 687, 723, 954, 1202
MspR9I	CCNGG	4 cut(s)	203, 383, 556, 638
MvaI	CCWGG	3 cut(s)	203, 556, 638
MvnI	CGCG	1 cut(s)	230
MwoI	GCNNNNNNNGC	8 cut(s)	58, 134, 734, 799, 866, 984, 1007, 1138
NciI	CCSGG	1 cut(s)	383
NcoI	CCATGG	1 cut(s)	771
NdeII	GATC	5 cut(s)	658, 708, 956, 1101, 1293
NheI	GCTAGC	1 cut(s)	61
NlaIV	GGNNCC	1 cut(s)	217
NmuCI	GTSAC	2 cut(s)	400, 1231
NspI	RCATGY	2 cut(s)	125, 146
PagI	TCATGA	1 cut(s)	1290
PciI	ACATGT	2 cut(s)	121, 142
PfeI	GAWTC	1 cut(s)	70
PkrI	GCNGC	7 cut(s)	267, 334, 337, 795, 798, 801, 1000
PleI	GAGTC	6 cut(s)	279, 310, 397, 839, 912, 1318
PpsI	GAGTC	6 cut(s)	279, 310, 397, 839, 912, 1318
Ppu21I	YACGTR	1 cut(s)	1173
PscI	ACATGT	2 cut(s)	121, 142
Psp1406I	AACGTT	1 cut(s)	504
Psp6I	CCWGG	3 cut(s)	201, 554, 636
PspGI	CCWGG	3 cut(s)	201, 554, 636
PspN4I	GGNNCC	1 cut(s)	217
PspPI	GGNCC	1 cut(s)	215
PstI	CTGCAG	2 cut(s)	798, 1134
PsuI	RGATCY	1 cut(s)	708
PvuII	CAGCTG	2 cut(s)	793, 998
RsaI	GTAC	2 cut(s)	888, 1175
RsaNI	GTAC	2 cut(s)	887, 1174
RseI	CAYNNNNRTG	1 cut(s)	1226
SaqAI	TTAA	3 cut(s)	156, 438, 462
SatI	GCNGC	7 cut(s)	266, 333, 336, 794, 797, 800, 999
Sau3AI	GATC	5 cut(s)	658, 708, 956, 1101, 1293
Sau96I	GGNCC	1 cut(s)	215
SchI	GAGTC	6 cut(s)	279, 310, 397, 839, 912, 1318
ScrFI	CCNGG	4 cut(s)	203, 383, 556, 638
SduI	GDGCHC	1 cut(s)	739
SfaNI	GCATC	2 cut(s)	436, 847
SfcI	CTRYAG	2 cut(s)	794, 1130
SmiMI	CAYNNNNRTG	1 cut(s)	1226
SpeI	ACTAGT	3 cut(s)	667, 874, 889
Sse9I	AATT	5 cut(s)	149, 476, 532, 1224, 1318
SsiI	CCGC	4 cut(s)	116, 862, 900, 1160
SspMI	CTAG	7 cut(s)	62, 548, 668, 852, 875, 890, 1278
StyD4I	CCNGG	4 cut(s)	201, 381, 554, 636
StyI	CCWWGG	1 cut(s)	771
TaaI	ACNGT	3 cut(s)	15, 242, 468
TaiI	ACGT	2 cut(s)	507, 1175
TaqI	TCGA	6 cut(s)	147, 393, 702, 731, 1073, 1327
TasI	AATT	5 cut(s)	149, 476, 532, 1224, 1318
TatI	WGTACW	1 cut(s)	886
TfiI	GAWTC	1 cut(s)	70
Tru1I	TTAA	3 cut(s)	156, 438, 462
Tru9I	TTAA	3 cut(s)	156, 438, 462
TscAI	CASTG	2 cut(s)	871, 985
TseFI	GTSAC	2 cut(s)	400, 1231
TseI	GCWGC	7 cut(s)	265, 332, 335, 793, 796, 799, 998
Tsp45I	GTSAC	2 cut(s)	400, 1231
TspDTI	ATGAA	6 cut(s)	212, 311, 314, 530, 1005, 1324
TspGWI	ACGGA	1 cut(s)	315
TspRI	CASTG	2 cut(s)	871, 985
XapI	RAATTY	1 cut(s)	149
XceI	RCATGY	2 cut(s)	125, 146
XcmI	CCANNNNNNNNNTGG	2 cut(s)	209, 244
XmiI	GTMKAC	1 cut(s)	1164
XspI	CTAG	7 cut(s)	62, 548, 668, 852, 875, 890, 1278

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.