pycom07g21860

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002292
Physical Location & Seq
Forward (+)
23381479 .. 23382190
712 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g21860.1

Sequence Viewer

Length: 510 bp
ATGGTTAGTTGGCAATGGAACGACTCCAAGTGCATTGGAAGTAAACACTGTAAACCAGAAGACTCTCCCAGCGATGCACGCACGGCGCTGCTAGTTATTGCTGTACTAGTGGCCACCACCACCTTTCAAGCCGGACTCAGCCCTCTGATGGGCATTTGGCAGGACAGCCCTGGATCAAACATGAATGGGACTGGCAGTGGGAGCAATGAACCAGCGCACACAGCAGGGCAGTCCATCACGAGTTCTTACGGCATATTAGGATTTTTCTTCTTTTACACTTCCAACTCGATTGGACTTTCGTTTTCGCTCTACATGATCCATGTCCTCACCACCAATTTCCCTCTGCAGTTGGAGCTTAACATTTGTATGTTCACGATGTTCTTGACGTACTATTCTGCAATTTTCGAAATTGGACCGGAGAACATGTCCCATCCTTTGTTCGCTTTAACTATGCCAGCGTTGGTAAGGGTGTGGTACGCGAAATGCACCCGCTTAGATGACCTGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 PGG

Protein Analysis

170

Amino Acids

18.64

Weight (kDa)

5.37

Isoelectric Point (pI)

54.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 24 - 132 7e-12 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 479
AciI CCGC 1 cut(s) 490
AclWI GGATC 2 cut(s) 181, 310
AcoI YGGCCR 1 cut(s) 111
AfaI GTAC 3 cut(s) 105, 389, 476
AfiI CCNNNNNNNGG 2 cut(s) 148, 149
AflIII ACRYGT 1 cut(s) 423
AgsI TTSAA 1 cut(s) 128
AhlI ACTAGT 1 cut(s) 106
AjnI CCWGG 1 cut(s) 169
AluBI AGCT 1 cut(s) 355
AluI AGCT 1 cut(s) 355
AlwI GGATC 2 cut(s) 181, 310
AoxI GGCC 1 cut(s) 111
ApeKI GCWGC 1 cut(s) 88
AspLEI GCGC 2 cut(s) 88, 217
AspS9I GGNCC 1 cut(s) 413
AsuHPI GGTGA 1 cut(s) 319
AsuII TTCGAA 1 cut(s) 405
AvaII GGWCC 1 cut(s) 413
BalI TGGCCA 1 cut(s) 113
BauI CACGAG 1 cut(s) 238
BbsI GAAGAC 1 cut(s) 66
BbvI GCAGC 1 cut(s) 75
BccI CCATC 3 cut(s) 142, 242, 438
BceAI ACGGC 2 cut(s) 99, 265
BciT130I CCWGG 1 cut(s) 171
BcuI ACTAGT 1 cut(s) 106
BfaI CTAG 2 cut(s) 92, 107
BfmI CTRYAG 2 cut(s) 344, 503
BfoI RGCGCY 1 cut(s) 89
BisI GCNGC 1 cut(s) 89
BlsI GCNGC 1 cut(s) 90
Bme1390I CCNGG 1 cut(s) 171
Bme18I GGWCC 1 cut(s) 413
BmgT120I GGNCC 1 cut(s) 413
BmrFI CCNGG 1 cut(s) 171
BmsI GCATC 1 cut(s) 64
BpiI GAAGAC 1 cut(s) 66
Bpu14I TTCGAA 1 cut(s) 405
BsaJI CCNNGG 1 cut(s) 169
BsaWI WCCGGW 1 cut(s) 415
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 149
Bse1I ACTGG 1 cut(s) 196
Bse3DI GCAATG 2 cut(s) 20, 211
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 1 cut(s) 169
BseGI GGATG 1 cut(s) 430
BseLI CCNNNNNNNGG 2 cut(s) 148, 149
BseMI GCAATG 2 cut(s) 20, 211
BseMII CTCAG 1 cut(s) 151
BseNI ACTGG 1 cut(s) 196
BseXI GCAGC 1 cut(s) 75
BseYI CCCAGC 1 cut(s) 68
Bsh1236I CGCG 1 cut(s) 479
BshFI GGCC 1 cut(s) 113
BsiSI CCGG 2 cut(s) 132, 416
BslFI GGGAC 2 cut(s) 202, 412
BslI CCNNNNNNNGG 2 cut(s) 148, 149
BsmFI GGGAC 2 cut(s) 202, 412
BsnI GGCC 1 cut(s) 113
Bsp119I TTCGAA 1 cut(s) 405
Bsp143I GATC 2 cut(s) 173, 315
BspACI CCGC 1 cut(s) 490
BspANI GGCC 1 cut(s) 113
BspCNI CTCAG 1 cut(s) 150
BspFNI CGCG 1 cut(s) 479
BspMAI CTGCAG 2 cut(s) 348, 507
BspPI GGATC 2 cut(s) 181, 310
BspT104I TTCGAA 1 cut(s) 405
BsrDI GCAATG 2 cut(s) 20, 211
BsrI ACTGG 1 cut(s) 196
BssECI CCNNGG 1 cut(s) 169
BssMI GATC 2 cut(s) 173, 315
BssSI CACGAG 1 cut(s) 238
Bst2BI CACGAG 1 cut(s) 238
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 1 cut(s) 50
BstBI TTCGAA 1 cut(s) 405
BstC8I GCNNGC 2 cut(s) 79, 456
BstDEI CTNAG 2 cut(s) 137, 493
BstF5I GGATG 1 cut(s) 430
BstFNI CGCG 1 cut(s) 479
BstH2I RGCGCY 1 cut(s) 89
BstHHI GCGC 2 cut(s) 88, 217
BstKTI GATC 2 cut(s) 176, 318
BstMBI GATC 2 cut(s) 173, 315
BstMWI GCNNNNNNNGC 5 cut(s) 78, 83, 201, 221, 352
BstNI CCWGG 1 cut(s) 171
BstNSI RCATGY 1 cut(s) 427
BstSCI CCNGG 1 cut(s) 169
BstSFI CTRYAG 2 cut(s) 344, 503
BstUI CGCG 1 cut(s) 479
BstV1I GCAGC 1 cut(s) 75
BstV2I GAAGAC 1 cut(s) 66
BsuRI GGCC 1 cut(s) 113
BtgZI GCGATG 1 cut(s) 87
BtsCI GGATG 1 cut(s) 430
BtsI GCAGTG 1 cut(s) 202
BtsIMutI CAGTG 2 cut(s) 46, 202
Cac8I GCNNGC 2 cut(s) 79, 456
CfoI GCGC 2 cut(s) 88, 217
Cfr13I GGNCC 1 cut(s) 413
Csp6I GTAC 3 cut(s) 104, 388, 475
CviAII CATG 4 cut(s) 181, 313, 320, 424
CviJI RGCY 5 cut(s) 113, 131, 141, 168, 355
CviKI_1 RGCY 5 cut(s) 113, 131, 141, 168, 355
CviQI GTAC 3 cut(s) 104, 388, 475
DdeI CTNAG 2 cut(s) 137, 493
DpnI GATC 2 cut(s) 175, 317
DpnII GATC 2 cut(s) 173, 315
EaeI YGGCCR 1 cut(s) 111
Eco47I GGWCC 1 cut(s) 413
EcoRII CCWGG 1 cut(s) 169
FaeI CATG 4 cut(s) 184, 316, 323, 427
FaiI YATR 7 cut(s) 182, 254, 314, 321, 368, 425, 452
FaqI GGGAC 2 cut(s) 202, 412
FatI CATG 4 cut(s) 180, 312, 319, 423
FauI CCCGC 1 cut(s) 497
Fnu4HI GCNGC 1 cut(s) 89
FokI GGATG 1 cut(s) 417
Fsp4HI GCNGC 1 cut(s) 89
FspBI CTAG 2 cut(s) 92, 107
GlaI GCGC 2 cut(s) 87, 216
GluI GCNGC 1 cut(s) 89
GsaI CCCAGC 1 cut(s) 72
HaeII RGCGCY 1 cut(s) 89
HaeIII GGCC 1 cut(s) 113
HapII CCGG 2 cut(s) 132, 416
HhaI GCGC 2 cut(s) 88, 217
Hin1II CATG 4 cut(s) 184, 316, 323, 427
Hin6I GCGC 2 cut(s) 86, 215
HinP1I GCGC 2 cut(s) 86, 215
HinfI GANTC 3 cut(s) 23, 62, 135
HpaII CCGG 2 cut(s) 132, 416
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 3 cut(s) 44, 53, 372
Hpy188I TCNGA 1 cut(s) 147
Hpy188III TCNNGA 3 cut(s) 238, 373, 382
Hpy8I GTNNAC 3 cut(s) 44, 53, 372
HpyCH4III ACNGT 1 cut(s) 50
HpyCH4IV ACGT 1 cut(s) 386
HpyCH4V TGCA 6 cut(s) 33, 77, 346, 398, 486, 505
HpyF10VI GCNNNNNNNGC 5 cut(s) 78, 83, 201, 221, 352
HpyF3I CTNAG 2 cut(s) 137, 493
HpySE526I ACGT 1 cut(s) 386
Hsp92II CATG 4 cut(s) 184, 316, 323, 427
HspAI GCGC 2 cut(s) 86, 215
Kzo9I GATC 2 cut(s) 173, 315
LmnI GCTCC 2 cut(s) 201, 352
Lsp1109I GCAGC 1 cut(s) 75
LweI GCATC 1 cut(s) 64
MaeI CTAG 2 cut(s) 92, 107
MaeII ACGT 1 cut(s) 386
MalI GATC 2 cut(s) 175, 317
MboI GATC 2 cut(s) 173, 315
MboII GAAGA 2 cut(s) 71, 259
MlsI TGGCCA 1 cut(s) 113
MluCI AATT 3 cut(s) 334, 399, 408
MluNI TGGCCA 1 cut(s) 113
MlyI GAGTC 3 cut(s) 17, 56, 129
MmeI TCCRAC 2 cut(s) 306, 330
MnlI CCTC 3 cut(s) 153, 335, 351
Mox20I TGGCCA 1 cut(s) 113
MscI TGGCCA 1 cut(s) 113
MseI TTAA 2 cut(s) 357, 446
MslI CAYNNNNRTG 1 cut(s) 365
Msp20I TGGCCA 1 cut(s) 113
MspI CCGG 2 cut(s) 132, 416
MspR9I CCNGG 1 cut(s) 171
MvaI CCWGG 1 cut(s) 171
MvnI CGCG 1 cut(s) 479
MwoI GCNNNNNNNGC 5 cut(s) 78, 83, 201, 221, 352
NdeII GATC 2 cut(s) 173, 315
NlaIII CATG 4 cut(s) 184, 316, 323, 427
NspI RCATGY 1 cut(s) 427
NspV TTCGAA 1 cut(s) 405
PciI ACATGT 1 cut(s) 423
PkrI GCNGC 1 cut(s) 90
PleI GAGTC 3 cut(s) 17, 56, 129
PpsI GAGTC 3 cut(s) 17, 56, 129
PscI ACATGT 1 cut(s) 423
Psp6I CCWGG 1 cut(s) 169
PspFI CCCAGC 1 cut(s) 68
PspGI CCWGG 1 cut(s) 169
PspPI GGNCC 1 cut(s) 413
PstI CTGCAG 2 cut(s) 348, 507
RsaI GTAC 3 cut(s) 105, 389, 476
RsaNI GTAC 3 cut(s) 104, 388, 475
RseI CAYNNNNRTG 1 cut(s) 365
SaqAI TTAA 2 cut(s) 357, 446
SatI GCNGC 1 cut(s) 89
Sau3AI GATC 2 cut(s) 173, 315
Sau96I GGNCC 1 cut(s) 413
SchI GAGTC 3 cut(s) 17, 56, 129
ScrFI CCNGG 1 cut(s) 171
SetI ASST 4 cut(s) 125, 357, 389, 504
SfaNI GCATC 1 cut(s) 64
SfcI CTRYAG 2 cut(s) 344, 503
SfuI TTCGAA 1 cut(s) 405
SinI GGWCC 1 cut(s) 413
SmiMI CAYNNNNRTG 1 cut(s) 365
SpeI ACTAGT 1 cut(s) 106
Sse9I AATT 3 cut(s) 334, 399, 408
SsiI CCGC 1 cut(s) 490
SspMI CTAG 2 cut(s) 92, 107
StyD4I CCNGG 1 cut(s) 169
TaaI ACNGT 1 cut(s) 50
TaiI ACGT 1 cut(s) 389
TaqI TCGA 2 cut(s) 287, 405
TasI AATT 3 cut(s) 334, 399, 408
TatI WGTACW 1 cut(s) 103
Tru1I TTAA 2 cut(s) 357, 446
Tru9I TTAA 2 cut(s) 357, 446
TscAI CASTG 2 cut(s) 53, 202
TseI GCWGC 1 cut(s) 88
TspDTI ATGAA 2 cut(s) 197, 222
TspRI CASTG 2 cut(s) 53, 202
VpaK11BI GGWCC 1 cut(s) 413
XceI RCATGY 1 cut(s) 427
XspI CTAG 2 cut(s) 92, 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.