pycom12g21410

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002292
Physical Location & Seq
Reverse (-)
22577451 .. 22577783
333 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g21410.1

Sequence Viewer

Length: 333 bp
ATGGGTTCTTATAGTGAAGTTTCATTCGTCGTTTTTGTGGTTGTCAACTCGATTGGATTTTACTTCTCTCTTTACACCATGATCGTCCTCACAGCCAATTTCCCTCTCCAGTTGGAGCTTCAAATCTGCATCATAGCGGTATACACCACCTACAGCACTGCGGTTGTCAACATTGCACCATCGTATAACACCTGGCTCTTTATCACCGTGTTCACGTCGGTTTTGCCTTTTTTGGTCGGACTTGCTGCCAAATGGGCTAGACACCCGGTCACAATCAGGCTTACAAAACCTCTCGGAAAATGGATCCTTCGGTTCTTTGAATTAATTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 PGG

Protein Analysis

111

Amino Acids

12.58

Weight (kDa)

9.25

Isoelectric Point (pI)

30.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 141
AciI CCGC 2 cut(s) 137, 161
AclWI GGATC 2 cut(s) 298, 311
AgsI TTSAA 2 cut(s) 122, 320
AhdI GACNNNNNGTC 1 cut(s) 266
AjiI CACGTC 1 cut(s) 216
AjnI CCWGG 1 cut(s) 191
AluBI AGCT 1 cut(s) 118
AluI AGCT 1 cut(s) 118
AlwI GGATC 2 cut(s) 298, 311
ApeKI GCWGC 1 cut(s) 245
AseI ATTAAT 1 cut(s) 323
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 1 cut(s) 196
BamHI GGATCC 1 cut(s) 303
BbvI GCAGC 1 cut(s) 232
BccI CCATC 1 cut(s) 187
BciT130I CCWGG 1 cut(s) 193
BcnI CCSGG 1 cut(s) 266
BfaI CTAG 1 cut(s) 258
BfmI CTRYAG 1 cut(s) 151
BglI GCCNNNNNGGC 1 cut(s) 254
BisI GCNGC 1 cut(s) 246
BlsI GCNGC 1 cut(s) 247
Bme1390I CCNGG 2 cut(s) 193, 266
BmeRI GACNNNNNGTC 1 cut(s) 266
BmgBI CACGTC 1 cut(s) 216
BmiI GGNNCC 1 cut(s) 305
BmrFI CCNGG 2 cut(s) 193, 266
BmsI GCATC 1 cut(s) 138
BpmI CTGGAG 1 cut(s) 92
BpuMI CCSGG 1 cut(s) 266
Bse1I ACTGG 1 cut(s) 109
Bse3DI GCAATG 1 cut(s) 171
BseBI CCWGG 1 cut(s) 193
BseMI GCAATG 1 cut(s) 171
BseNI ACTGG 1 cut(s) 109
BseXI GCAGC 1 cut(s) 232
BsiSI CCGG 1 cut(s) 266
Bsp143I GATC 2 cut(s) 81, 303
BspACI CCGC 2 cut(s) 137, 161
BspLI GGNNCC 1 cut(s) 305
BspPI GGATC 2 cut(s) 298, 311
BsrDI GCAATG 1 cut(s) 171
BsrI ACTGG 1 cut(s) 109
BssMI GATC 2 cut(s) 81, 303
BssNAI GTATAC 1 cut(s) 142
Bst1107I GTATAC 1 cut(s) 142
Bst2UI CCWGG 1 cut(s) 193
Bst4CI ACNGT 1 cut(s) 208
BstKTI GATC 2 cut(s) 84, 306
BstMBI GATC 2 cut(s) 81, 303
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 1 cut(s) 193
BstSCI CCNGG 2 cut(s) 191, 264
BstSFI CTRYAG 1 cut(s) 151
BstV1I GCAGC 1 cut(s) 232
BstX2I RGATCY 1 cut(s) 303
BstYI RGATCY 1 cut(s) 303
BstZ17I GTATAC 1 cut(s) 142
BtrI CACGTC 1 cut(s) 216
BtsI GCAGTG 1 cut(s) 156
BtsIMutI CAGTG 1 cut(s) 156
CviAII CATG 1 cut(s) 79
CviJI RGCY 5 cut(s) 95, 118, 196, 257, 280
CviKI_1 RGCY 5 cut(s) 95, 118, 196, 257, 280
DpnI GATC 2 cut(s) 83, 305
DpnII GATC 2 cut(s) 81, 303
DriI GACNNNNNGTC 1 cut(s) 266
Eam1105I GACNNNNNGTC 1 cut(s) 266
EcoRII CCWGG 1 cut(s) 191
FaeI CATG 1 cut(s) 82
FaiI YATR 5 cut(s) 12, 80, 134, 142, 186
FatI CATG 1 cut(s) 78
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 1 cut(s) 246
Fsp4HI GCNGC 1 cut(s) 246
FspBI CTAG 1 cut(s) 258
GluI GCNGC 1 cut(s) 246
GsuI CTGGAG 1 cut(s) 92
HapII CCGG 1 cut(s) 266
Hin1II CATG 1 cut(s) 82
HincII GTYRAC 2 cut(s) 46, 169
HindII GTYRAC 2 cut(s) 46, 169
HpaII CCGG 1 cut(s) 266
HphI GGTGA 1 cut(s) 196
Hpy166II GTNNAC 4 cut(s) 46, 142, 169, 213
Hpy188I TCNGA 2 cut(s) 239, 296
Hpy8I GTNNAC 4 cut(s) 46, 142, 169, 213
Hpy99I CGWCG 2 cut(s) 32, 220
HpyAV CCTTC 1 cut(s) 317
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 1 cut(s) 215
HpyCH4V TGCA 2 cut(s) 129, 176
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpySE526I ACGT 1 cut(s) 215
Hsp92II CATG 1 cut(s) 82
Kzo9I GATC 2 cut(s) 81, 303
LmnI GCTCC 1 cut(s) 115
LpnPI CCDG 5 cut(s) 122, 178, 205, 262, 279
Lsp1109I GCAGC 1 cut(s) 232
LweI GCATC 1 cut(s) 138
MaeI CTAG 1 cut(s) 258
MaeII ACGT 1 cut(s) 215
MaeIII GTNAC 1 cut(s) 268
MalI GATC 2 cut(s) 83, 305
MboI GATC 2 cut(s) 81, 303
MflI RGATCY 1 cut(s) 303
MluCI AATT 3 cut(s) 97, 320, 324
MmeI TCCRAC 2 cut(s) 93, 217
MnlI CCTC 3 cut(s) 98, 114, 300
MseI TTAA 1 cut(s) 323
MspI CCGG 1 cut(s) 266
MspR9I CCNGG 2 cut(s) 193, 266
MvaI CCWGG 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 254
NciI CCSGG 1 cut(s) 266
NdeII GATC 2 cut(s) 81, 303
NlaIII CATG 1 cut(s) 82
NlaIV GGNNCC 1 cut(s) 305
NmuCI GTSAC 1 cut(s) 268
PkrI GCNGC 1 cut(s) 247
PshBI ATTAAT 1 cut(s) 323
Psp6I CCWGG 1 cut(s) 191
PspGI CCWGG 1 cut(s) 191
PspN4I GGNNCC 1 cut(s) 305
PsuI RGATCY 1 cut(s) 303
SaqAI TTAA 1 cut(s) 323
SatI GCNGC 1 cut(s) 246
Sau3AI GATC 2 cut(s) 81, 303
ScrFI CCNGG 2 cut(s) 193, 266
SetI ASST 5 cut(s) 120, 152, 194, 218, 292
SfaNI GCATC 1 cut(s) 138
SfcI CTRYAG 1 cut(s) 151
Sse9I AATT 3 cut(s) 97, 320, 324
SsiI CCGC 2 cut(s) 137, 161
SspMI CTAG 1 cut(s) 258
StyD4I CCNGG 2 cut(s) 191, 264
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 1 cut(s) 218
TaqI TCGA 1 cut(s) 50
TasI AATT 3 cut(s) 97, 320, 324
Tru1I TTAA 1 cut(s) 323
Tru9I TTAA 1 cut(s) 323
TscAI CASTG 1 cut(s) 163
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 1 cut(s) 245
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 1 cut(s) 12
TspRI CASTG 1 cut(s) 163
VspI ATTAAT 1 cut(s) 323
XmiI GTMKAC 1 cut(s) 141
XspI CTAG 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.