pycom07g21780

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002292
Physical Location & Seq
Forward (+)
23344974 .. 23345876
903 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g21780.2

Sequence Viewer

Length: 669 bp
ATGGATTCGAGGCTGTCTGAGGCTTCTAAAACAGGAGACATCCAGCTCTTGCACCAGTTGATGGCAGAGAATCCACTTCTGCTCCATAACCTTGCCCTAACTTCCATCGAGAACCCGCTACATGTCGCTTCGCTTGCCGGACACGTAGAATTTGTTCAGGAGATCGTGAAGTTGAGACCAGCTTTTGTGCATGAAATGAACCAGGATGGGTTCAGCCCCATGCACATCGCGGCAGCCAACGGGTATTTGGAGGTTGTAAGGGAGCTGCTGAGAGTTGACCCGAGACTCTGCCGGATGAAAGGAAGAGACCAATGGACTCCTCTTCATTATGCTGCTGCTAGAGGGAGGGGTGATGTTGTCGGAGAGATGGTTTTGGCTTGCCCGGAAAGTCTCGAAGACGTTACTCTGCATGAGGAGACTTCGTTGCACCTTGCTGTTAATAACTGCCAGTTTGAAGCAGTTAAAATTTTGGTTGAGTTGGTCATCGAACTGAGGAGGGACAACGTTTTGAATATGAAGGACAAATATGGCAACTCAGTTCTTCACGTAGCAACCTGGAAGAAACAACACCAGGTGGTGGAATGGATAGTTGGCAATAGAACAACTCCAAGTGCATTGGAAGTAAACATTGTAAACCAGAGTGGTCTAACACCTCTCGATCGATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

223

Amino Acids

24.9

Weight (kDa)

5.86

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 61, 577
AccII CGCG 1 cut(s) 230
AciI CCGC 2 cut(s) 116, 230
AclI AACGTT 1 cut(s) 504
AcsI RAATTY 2 cut(s) 149, 465
AdeI CACNNNGTG 1 cut(s) 574
AfiI CCNNNNNNNGG 2 cut(s) 61, 577
AflIII ACRYGT 2 cut(s) 121, 142
AgsI TTSAA 2 cut(s) 455, 511
AjnI CCWGG 3 cut(s) 201, 554, 570
AjuI GAANNNNNNNTTGG 2 cut(s) 573, 605
AluBI AGCT 3 cut(s) 46, 182, 265
AluI AGCT 3 cut(s) 46, 182, 265
Alw26I GTCTC 6 cut(s) 30, 169, 277, 300, 395, 410
Ama87I CYCGRG 1 cut(s) 280
ApeKI GCWGC 4 cut(s) 233, 265, 332, 335
ApoI RAATTY 2 cut(s) 149, 465
Asp700I GAANNNNTTC 1 cut(s) 153
AsuC2I CCSGG 1 cut(s) 383
AsuHPI GGTGA 1 cut(s) 362
AvaI CYCGRG 1 cut(s) 280
BbsI GAAGAC 1 cut(s) 402
BbvI GCAGC 4 cut(s) 245, 252, 319, 322
BccI CCATC 4 cut(s) 55, 113, 200, 361
BciT130I CCWGG 3 cut(s) 203, 556, 572
BcnI CCSGG 1 cut(s) 383
BcoDI GTCTC 6 cut(s) 30, 169, 277, 300, 395, 410
BfaI CTAG 1 cut(s) 339
BisI GCNGC 5 cut(s) 231, 234, 266, 333, 336
BlsI GCNGC 5 cut(s) 232, 235, 267, 334, 337
Bme1390I CCNGG 4 cut(s) 203, 383, 556, 572
BmeT110I CYCGRG 1 cut(s) 280
BmrFI CCNGG 4 cut(s) 203, 383, 556, 572
BpiI GAAGAC 1 cut(s) 402
BpuMI CCSGG 1 cut(s) 383
Bsa29I ATCGAT 1 cut(s) 661
BsaAI YACGTR 2 cut(s) 145, 547
BsaI GGTCTC 2 cut(s) 169, 300
BsaXI ACNNNNNCTCC 6 cut(s) 66, 96, 354, 384, 407, 437
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 577
Bse1I ACTGG 2 cut(s) 55, 448
BseBI CCWGG 3 cut(s) 203, 556, 572
BseCI ATCGAT 1 cut(s) 661
BseGI GGATG 3 cut(s) 39, 211, 300
BseLI CCNNNNNNNGG 2 cut(s) 61, 577
BseMII CTCAG 4 cut(s) 9, 260, 482, 549
BseNI ACTGG 2 cut(s) 55, 448
BseRI GAGGAG 3 cut(s) 309, 428, 508
BseXI GCAGC 4 cut(s) 245, 252, 319, 322
Bsh1236I CGCG 1 cut(s) 230
Bsh1285I CGRYCG 1 cut(s) 661
BshVI ATCGAT 1 cut(s) 661
BsiEI CGRYCG 1 cut(s) 661
BsiHKCI CYCGRG 1 cut(s) 280
BsiSI CCGG 3 cut(s) 138, 292, 383
BslFI GGGAC 1 cut(s) 512
BslI CCNNNNNNNGG 2 cut(s) 61, 577
BsmAI GTCTC 6 cut(s) 30, 169, 277, 300, 395, 410
BsmFI GGGAC 1 cut(s) 512
Bso31I GGTCTC 2 cut(s) 169, 300
BsoBI CYCGRG 1 cut(s) 280
Bsp143I GATC 3 cut(s) 162, 658, 662
BspACI CCGC 2 cut(s) 116, 230
BspCNI CTCAG 4 cut(s) 10, 261, 483, 548
BspDI ATCGAT 1 cut(s) 661
BspFNI CGCG 1 cut(s) 230
BspHI TCATGA 1 cut(s) 665
BspTNI GGTCTC 2 cut(s) 169, 300
BsrI ACTGG 2 cut(s) 55, 448
BssMI GATC 3 cut(s) 162, 658, 662
Bst2UI CCWGG 3 cut(s) 203, 556, 572
Bst6I CTCTTC 2 cut(s) 298, 327
BstBAI YACGTR 2 cut(s) 145, 547
BstC8I GCNNGC 2 cut(s) 135, 379
BstDEI CTNAG 4 cut(s) 18, 269, 491, 535
BstF5I GGATG 3 cut(s) 39, 211, 300
BstFNI CGCG 1 cut(s) 230
BstKTI GATC 3 cut(s) 165, 661, 665
BstMAI GTCTC 6 cut(s) 30, 169, 277, 300, 395, 410
BstMBI GATC 3 cut(s) 162, 658, 662
BstMCI CGRYCG 1 cut(s) 661
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNI CCWGG 3 cut(s) 203, 556, 572
BstNSI RCATGY 1 cut(s) 125
BstSCI CCNGG 4 cut(s) 201, 381, 554, 570
BstUI CGCG 1 cut(s) 230
BstV1I GCAGC 4 cut(s) 245, 252, 319, 322
BstV2I GAAGAC 1 cut(s) 402
Bsu15I ATCGAT 1 cut(s) 661
BsuTUI ATCGAT 1 cut(s) 661
BtgZI GCGATG 1 cut(s) 211
BtsCI GGATG 3 cut(s) 39, 211, 300
Cac8I GCNNGC 2 cut(s) 135, 379
CciI TCATGA 1 cut(s) 665
ClaI ATCGAT 1 cut(s) 661
CsiI ACCWGGT 1 cut(s) 570
CviAII CATG 5 cut(s) 122, 191, 220, 410, 666
CviJI RGCY 8 cut(s) 13, 23, 46, 182, 216, 236, 265, 377
CviKI_1 RGCY 8 cut(s) 13, 23, 46, 182, 216, 236, 265, 377
DdeI CTNAG 4 cut(s) 18, 269, 491, 535
DpnI GATC 3 cut(s) 164, 660, 664
DpnII GATC 3 cut(s) 162, 658, 662
DraIII CACNNNGTG 1 cut(s) 574
Eam1104I CTCTTC 2 cut(s) 298, 327
EarI CTCTTC 2 cut(s) 298, 327
Eco31I GGTCTC 2 cut(s) 169, 300
Eco88I CYCGRG 1 cut(s) 280
EcoRII CCWGG 3 cut(s) 201, 554, 570
FaeI CATG 5 cut(s) 125, 194, 223, 413, 669
FaiI YATR 9 cut(s) 87, 123, 192, 221, 330, 411, 515, 528, 667
FaqI GGGAC 1 cut(s) 512
FatI CATG 5 cut(s) 121, 190, 219, 409, 665
FauI CCCGC 1 cut(s) 123
Fnu4HI GCNGC 5 cut(s) 231, 234, 266, 333, 336
FokI GGATG 3 cut(s) 26, 218, 307
Fsp4HI GCNGC 5 cut(s) 231, 234, 266, 333, 336
FspBI CTAG 1 cut(s) 339
GluI GCNGC 5 cut(s) 231, 234, 266, 333, 336
HapII CCGG 3 cut(s) 138, 292, 383
Hin1II CATG 5 cut(s) 125, 194, 223, 413, 669
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 4 cut(s) 5, 70, 285, 316
HpaII CCGG 3 cut(s) 138, 292, 383
HphI GGTGA 1 cut(s) 362
Hpy166II GTNNAC 3 cut(s) 277, 625, 634
Hpy188I TCNGA 2 cut(s) 19, 362
Hpy188III TCNNGA 6 cut(s) 109, 158, 166, 392, 656, 666
Hpy8I GTNNAC 3 cut(s) 277, 625, 634
HpyAV CCTTC 1 cut(s) 511
HpyCH4IV ACGT 4 cut(s) 144, 399, 504, 546
HpyCH4V TGCA 6 cut(s) 52, 190, 223, 409, 427, 614
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 4 cut(s) 18, 269, 491, 535
HpySE526I ACGT 4 cut(s) 144, 399, 504, 546
Hsp92II CATG 5 cut(s) 125, 194, 223, 413, 669
Kzo9I GATC 3 cut(s) 162, 658, 662
LmnI GCTCC 2 cut(s) 87, 262
Lsp1109I GCAGC 4 cut(s) 245, 252, 319, 322
MabI ACCWGGT 1 cut(s) 570
MaeI CTAG 1 cut(s) 339
MaeII ACGT 4 cut(s) 144, 399, 504, 546
MaeIII GTNAC 1 cut(s) 400
MalI GATC 3 cut(s) 164, 660, 664
MboI GATC 3 cut(s) 162, 658, 662
MboII GAAGA 5 cut(s) 314, 315, 407, 533, 571
MluCI AATT 2 cut(s) 149, 465
MlyI GAGTC 2 cut(s) 279, 310
MmeI TCCRAC 1 cut(s) 340
MroXI GAANNNNTTC 1 cut(s) 153
MseI TTAA 2 cut(s) 438, 462
MspI CCGG 3 cut(s) 138, 292, 383
MspR9I CCNGG 4 cut(s) 203, 383, 556, 572
MvaI CCWGG 3 cut(s) 203, 556, 572
MvnI CGCG 1 cut(s) 230
MwoI GCNNNNNNNGC 1 cut(s) 134
NciI CCSGG 1 cut(s) 383
NdeII GATC 3 cut(s) 162, 658, 662
NlaIII CATG 5 cut(s) 125, 194, 223, 413, 669
NspI RCATGY 1 cut(s) 125
PagI TCATGA 1 cut(s) 665
PciI ACATGT 1 cut(s) 121
PdmI GAANNNNTTC 1 cut(s) 153
PfeI GAWTC 2 cut(s) 5, 70
PflMI CCANNNNNTGG 2 cut(s) 61, 577
PkrI GCNGC 5 cut(s) 232, 235, 267, 334, 337
Ple19I CGATCG 1 cut(s) 661
PleI GAGTC 2 cut(s) 279, 310
PpsI GAGTC 2 cut(s) 279, 310
Ppu21I YACGTR 2 cut(s) 145, 547
PscI ACATGT 1 cut(s) 121
Psp1406I AACGTT 1 cut(s) 504
Psp6I CCWGG 3 cut(s) 201, 554, 570
PspGI CCWGG 3 cut(s) 201, 554, 570
PsrI GAACNNNNNNTAC 2 cut(s) 138, 170
PvuI CGATCG 1 cut(s) 661
SaqAI TTAA 2 cut(s) 438, 462
SatI GCNGC 5 cut(s) 231, 234, 266, 333, 336
Sau3AI GATC 3 cut(s) 162, 658, 662
SchI GAGTC 2 cut(s) 279, 310
ScrFI CCNGG 4 cut(s) 203, 383, 556, 572
SexAI ACCWGGT 1 cut(s) 570
Sse9I AATT 2 cut(s) 149, 465
SsiI CCGC 2 cut(s) 116, 230
SspMI CTAG 1 cut(s) 339
StyD4I CCNGG 4 cut(s) 201, 381, 554, 570
TaiI ACGT 4 cut(s) 147, 402, 507, 549
TaqI TCGA 6 cut(s) 8, 108, 393, 486, 657, 661
TasI AATT 2 cut(s) 149, 465
TauI GCSGC 1 cut(s) 233
TfiI GAWTC 2 cut(s) 5, 70
Tru1I TTAA 2 cut(s) 438, 462
Tru9I TTAA 2 cut(s) 438, 462
TseI GCWGC 4 cut(s) 233, 265, 332, 335
TspDTI ATGAA 5 cut(s) 207, 212, 311, 314, 530
Van91I CCANNNNNTGG 2 cut(s) 61, 577
XapI RAATTY 2 cut(s) 149, 465
XceI RCATGY 1 cut(s) 125
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XmnI GAANNNNTTC 1 cut(s) 153
XspI CTAG 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.