pycom07g21970

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002292
Physical Location & Seq
Forward (+)
23441454 .. 23442062
609 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g21970.2

Sequence Viewer

Length: 558 bp
ATGAATAAATTGAGTGTACGACATGTGAATGTCCAACAACAAAGACTTCGGGGCTTGTTGTCGAATATCTGCAGTGCATCATTTCTGATAGATCAAATCGAGCCAATTCCCGCAAACTCCAATCCCACCACCACCTTTCCAGCCGGACTCAGCCCTCCGGGCGGCATTTGGCAGGACAGCACTGGATCAAACAAGAATGGGACTGGCAGTGGGAGCAATGAACGACCACACTCAGCAGGGAAGTCCATCATGGGTTCTAACCACACTTTGTTTCTCTACTTTGTGGCTTCCAACACGATTGGATTTTTTGTTTCACTGTACATGATCAATGTCCTCACCAGCAATTTCCCTTTGCAGTTGGAGCTTCAAATTTGTATTGTCGCGATGTACAGCACGTACAACACTGCAGTTTTCAGCATTGCACCGGAGAACGTGCGGTACTTAGTCCTTCTGTTCTCTGTAGTTTATAATGGTGTTTGTTCTATAAGTTGGCATCTTTGCCAACCGCAATCGATACTGTCGTTTGATACTGCAAATGAAAACCTGGTGGGTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 PGG

Protein Analysis

186

Amino Acids

20.12

Weight (kDa)

6.88

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 468
AccII CGCG 1 cut(s) 383
AciI CCGC 4 cut(s) 111, 162, 436, 506
AclWI GGATC 1 cut(s) 193
AcsI RAATTY 1 cut(s) 369
AfaI GTAC 5 cut(s) 18, 320, 389, 398, 440
AfiI CCNNNNNNNGG 1 cut(s) 161
AflIII ACRYGT 1 cut(s) 22
AgsI TTSAA 1 cut(s) 368
AjnI CCWGG 1 cut(s) 543
AluBI AGCT 1 cut(s) 364
AluI AGCT 1 cut(s) 364
AlwI GGATC 1 cut(s) 193
ApoI RAATTY 1 cut(s) 369
AsuC2I CCSGG 1 cut(s) 159
AsuHPI GGTGA 1 cut(s) 328
BaeI ACNNNNGTAYC 2 cut(s) 506, 539
BccI CCATC 1 cut(s) 254
BciT130I CCWGG 1 cut(s) 545
BclI TGATCA 1 cut(s) 324
BcnI CCSGG 1 cut(s) 159
BfmI CTRYAG 3 cut(s) 70, 405, 459
BglI GCCNNNNNGGC 1 cut(s) 159
BisI GCNGC 1 cut(s) 163
BlsI GCNGC 1 cut(s) 164
Bme1390I CCNGG 2 cut(s) 159, 545
BmrFI CCNGG 2 cut(s) 159, 545
BmsI GCATC 2 cut(s) 86, 502
BpuMI CCSGG 1 cut(s) 159
Bsa29I ATCGAT 1 cut(s) 512
BsaAI YACGTR 1 cut(s) 396
BsaWI WCCGGW 1 cut(s) 424
Bsc4I CCNNNNNNNGG 1 cut(s) 161
Bse1I ACTGG 2 cut(s) 187, 208
Bse3DI GCAATG 2 cut(s) 223, 417
BseBI CCWGG 1 cut(s) 545
BseCI ATCGAT 1 cut(s) 512
BseLI CCNNNNNNNGG 1 cut(s) 161
BseMI GCAATG 2 cut(s) 223, 417
BseMII CTCAG 2 cut(s) 163, 246
BseNI ACTGG 2 cut(s) 187, 208
Bsh1236I CGCG 1 cut(s) 383
BshVI ATCGAT 1 cut(s) 512
BsiSI CCGG 3 cut(s) 144, 158, 425
BslFI GGGAC 1 cut(s) 214
BslI CCNNNNNNNGG 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 214
Bsp1407I TGTACA 2 cut(s) 318, 387
Bsp143I GATC 3 cut(s) 91, 185, 324
Bsp68I TCGCGA 1 cut(s) 383
BspACI CCGC 4 cut(s) 111, 162, 436, 506
BspCNI CTCAG 2 cut(s) 162, 245
BspDI ATCGAT 1 cut(s) 512
BspFNI CGCG 1 cut(s) 383
BspMAI CTGCAG 2 cut(s) 74, 409
BspPI GGATC 1 cut(s) 193
BsrDI GCAATG 2 cut(s) 223, 417
BsrGI TGTACA 2 cut(s) 318, 387
BsrI ACTGG 2 cut(s) 187, 208
BssMI GATC 3 cut(s) 91, 185, 324
Bst2UI CCWGG 1 cut(s) 545
Bst4CI ACNGT 2 cut(s) 318, 519
BstAUI TGTACA 2 cut(s) 318, 387
BstBAI YACGTR 1 cut(s) 396
BstDEI CTNAG 3 cut(s) 149, 232, 442
BstFNI CGCG 1 cut(s) 383
BstKTI GATC 3 cut(s) 94, 188, 327
BstMBI GATC 3 cut(s) 91, 185, 324
BstMWI GCNNNNNNNGC 3 cut(s) 159, 213, 361
BstNI CCWGG 1 cut(s) 545
BstNSI RCATGY 1 cut(s) 26
BstSCI CCNGG 2 cut(s) 157, 543
BstSFI CTRYAG 3 cut(s) 70, 405, 459
BstUI CGCG 1 cut(s) 383
Bsu15I ATCGAT 1 cut(s) 512
BsuTUI ATCGAT 1 cut(s) 512
BtgZI GCGATG 1 cut(s) 398
BtsI GCAGTG 3 cut(s) 79, 214, 402
BtsIMutI CAGTG 5 cut(s) 79, 180, 214, 314, 402
BtuMI TCGCGA 1 cut(s) 383
ClaI ATCGAT 1 cut(s) 512
CsiI ACCWGGT 1 cut(s) 543
Csp6I GTAC 5 cut(s) 17, 319, 388, 397, 439
CviAII CATG 3 cut(s) 23, 250, 322
CviJI RGCY 6 cut(s) 54, 103, 143, 153, 287, 364
CviKI_1 RGCY 6 cut(s) 54, 103, 143, 153, 287, 364
CviQI GTAC 5 cut(s) 17, 319, 388, 397, 439
DdeI CTNAG 3 cut(s) 149, 232, 442
DpnI GATC 3 cut(s) 93, 187, 326
DpnII GATC 3 cut(s) 91, 185, 324
EcoRII CCWGG 1 cut(s) 543
FaeI CATG 3 cut(s) 26, 253, 325
FaiI YATR 5 cut(s) 24, 251, 323, 468, 485
FaqI GGGAC 1 cut(s) 214
FatI CATG 3 cut(s) 22, 249, 321
FauI CCCGC 1 cut(s) 118
FbaI TGATCA 1 cut(s) 324
Fnu4HI GCNGC 1 cut(s) 163
Fsp4HI GCNGC 1 cut(s) 163
GluI GCNGC 1 cut(s) 163
HapII CCGG 3 cut(s) 144, 158, 425
Hin1II CATG 3 cut(s) 26, 253, 325
HinfI GANTC 1 cut(s) 147
HpaII CCGG 3 cut(s) 144, 158, 425
HphI GGTGA 1 cut(s) 328
Hpy166II GTNNAC 1 cut(s) 17
Hpy188I TCNGA 1 cut(s) 87
Hpy188III TCNNGA 1 cut(s) 382
Hpy8I GTNNAC 1 cut(s) 17
HpyAV CCTTC 1 cut(s) 458
HpyCH4III ACNGT 2 cut(s) 318, 519
HpyCH4IV ACGT 2 cut(s) 395, 432
HpyCH4V TGCA 6 cut(s) 72, 77, 355, 407, 422, 533
HpyF10VI GCNNNNNNNGC 3 cut(s) 159, 213, 361
HpyF3I CTNAG 3 cut(s) 149, 232, 442
HpySE526I ACGT 2 cut(s) 395, 432
Hsp92II CATG 3 cut(s) 26, 253, 325
Ksp22I TGATCA 1 cut(s) 324
Kzo9I GATC 3 cut(s) 91, 185, 324
LmnI GCTCC 2 cut(s) 213, 361
LweI GCATC 2 cut(s) 86, 502
MabI ACCWGGT 1 cut(s) 543
MaeII ACGT 2 cut(s) 395, 432
MalI GATC 3 cut(s) 93, 187, 326
MboI GATC 3 cut(s) 91, 185, 324
MluCI AATT 4 cut(s) 8, 105, 343, 369
MlyI GAGTC 1 cut(s) 141
MmeI TCCRAC 3 cut(s) 58, 315, 339
MnlI CCTC 2 cut(s) 165, 344
MslI CAYNNNNRTG 1 cut(s) 27
MspI CCGG 3 cut(s) 144, 158, 425
MspR9I CCNGG 2 cut(s) 159, 545
MvaI CCWGG 1 cut(s) 545
MvnI CGCG 1 cut(s) 383
MwoI GCNNNNNNNGC 3 cut(s) 159, 213, 361
NciI CCSGG 1 cut(s) 159
NdeII GATC 3 cut(s) 91, 185, 324
NlaIII CATG 3 cut(s) 26, 253, 325
NruI TCGCGA 1 cut(s) 383
NspI RCATGY 1 cut(s) 26
PciI ACATGT 1 cut(s) 22
PcsI WCGNNNNNNNCGW 1 cut(s) 518
PkrI GCNGC 1 cut(s) 164
PleI GAGTC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 141
Ppu21I YACGTR 1 cut(s) 396
PscI ACATGT 1 cut(s) 22
PsiI TTATAA 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 543
PspGI CCWGG 1 cut(s) 543
PsrI GAACNNNNNNTAC 2 cut(s) 422, 454
PstI CTGCAG 2 cut(s) 74, 409
RruI TCGCGA 1 cut(s) 383
RsaI GTAC 5 cut(s) 18, 320, 389, 398, 440
RsaNI GTAC 5 cut(s) 17, 319, 388, 397, 439
RseI CAYNNNNRTG 1 cut(s) 27
SatI GCNGC 1 cut(s) 163
Sau3AI GATC 3 cut(s) 91, 185, 324
SchI GAGTC 1 cut(s) 141
ScrFI CCNGG 2 cut(s) 159, 545
SetI ASST 5 cut(s) 137, 366, 398, 435, 546
SexAI ACCWGGT 1 cut(s) 543
SfaNI GCATC 2 cut(s) 86, 502
SfcI CTRYAG 3 cut(s) 70, 405, 459
SmiMI CAYNNNNRTG 1 cut(s) 27
Sse9I AATT 4 cut(s) 8, 105, 343, 369
SsiI CCGC 4 cut(s) 111, 162, 436, 506
StyD4I CCNGG 2 cut(s) 157, 543
TaaI ACNGT 2 cut(s) 318, 519
TaiI ACGT 2 cut(s) 398, 435
TaqI TCGA 3 cut(s) 62, 99, 512
TasI AATT 4 cut(s) 8, 105, 343, 369
TatI WGTACW 2 cut(s) 318, 387
TauI GCSGC 1 cut(s) 165
TscAI CASTG 5 cut(s) 79, 187, 214, 321, 409
TspDTI ATGAA 3 cut(s) 17, 234, 552
TspRI CASTG 5 cut(s) 79, 187, 214, 321, 409
XapI RAATTY 1 cut(s) 369
XceI RCATGY 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.