pycom07g21800

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002292
Physical Location & Seq
Forward (+)
23349540 .. 23351583
2044 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g21800.2

Sequence Viewer

Length: 1302 bp
ATGGATTCGAGGCTGTCTGAGGCTTCTAAAACAGGAGATATCCAGCTCTTGCACCAGTTGATGGCAGAGAATCCACTTCTGCTCCATAACCTTGCCCTAACTTCCAGCGAGAACCCGCTACATGTCGCTTCACTTGCCGGACATGTCGAATTTGTTCAGGAGATCCTGAAGTTGAAACCAGCTTTTGTGCATGAAATGAACCAGGATGGGTTCAGCCCCATGCACATCGCGGCAGCCAACGGGTATTTGGAGGTTGTAAGGGAGCTGCTGATAGCTGACCCGAGACTCTGTCGGACGAAAGGAAGAGACCAATGGACTCCTCTTCATTATGCTGCTGCCAGAGGGAGGGGTGATGTTGTCAAAGAGATGGTTTTGGCTTGCCCGGAAAGTCTCGAAGACGTGACTCTATATGATGAGACTTCGTTGCACCTTGGTGTTAATAACTGCCAGTTTGAAGCAGTTAAAACTTTGGTTGAATCGGTCATGGAACTGAGGAAGGACAACGTTTTGAATATGAAGGACAAAAATGGCAATTCAGTTCTTCACGTAGCAACCTGGAAGAAACAACACCAGGTAGTGAAATGGTTAGTTGGCAATGGAAAAACTCCAAGTGCCTTGGAAGTAAACATTGTAAACCAGAGTGGTCTAACACCTCTTGATCTGCTACTAATCTTCCCGAGTGAAGCCGGCGATAGGGAAATTGAAGAGATCCTTCGTGGTGCCGGAGCTTCGAGAGCACAAGACATACAATATACTCCCTCTCATGGTCCCATGGAAACAAATACCCTTCAGCTGCAGCAGCCAAACAACCCGATGGAATACTTCAAGTTCAAAATGGGCAGAGACTCTCCCAGCGATGCACGCACGGCGCTGCTAGTTGTCGCTGTACTAGTGGCCACCGCCACCTTTCAAGCCGGACTCAGCCCCCCGGGCGGCGTTTGGCAGGACAGCACTGGATCAAACAAGAATGGAACTGGCAGTGGGAGCAATGAACCAGCACACTCAGCAGGGGAGTCCGTCATGGGTTCTTACCACATATCGTTTGTCGGCTTTATGGTTCTCAACTCGATTGGATTTTTTGTTTCACTTGACATGATCAAGGTCCTCACCATCAATTTCCCTTTGCAGTTGCAGCTTCGAATTTGTATGGTCGCGATTTACTTCACGTACAGCAGTGCAGTTATCAGCATTGCACCGAACACCATGATCAGTGTCTTCTGCGTCTTTGCGTTCTTTGTTTGTTTCCCAATTGCTGTTTCACATGCTTTCCGTTGCCGCATCAGGAACTCGATCAGGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

434

Amino Acids

47.58

Weight (kDa)

6.16

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 719
AccB7I CCANNNNNTGG 1 cut(s) 61
AccII CGCG 2 cut(s) 230, 1154
AciI CCGC 5 cut(s) 116, 230, 900, 933, 1276
AclI AACGTT 1 cut(s) 504
AclWI GGATC 3 cut(s) 157, 703, 964
AcoI YGGCCR 1 cut(s) 894
AcsI RAATTY 2 cut(s) 149, 1140
AcuI CTGAAG 2 cut(s) 188, 773
AfaI GTAC 2 cut(s) 888, 1169
AfiI CCNNNNNNNGG 5 cut(s) 61, 345, 693, 764, 932
AflIII ACRYGT 2 cut(s) 121, 142
AgsI TTSAA 8 cut(s) 175, 455, 476, 511, 704, 826, 832, 911
AhlI ACTAGT 1 cut(s) 889
AjiI CACGTC 1 cut(s) 400
AjnI CCWGG 3 cut(s) 201, 554, 570
AjuI GAANNNNNNNTTGG 2 cut(s) 1240, 1272
AleI CACNNNNGTG 1 cut(s) 432
AluBI AGCT 7 cut(s) 46, 182, 265, 275, 728, 793, 1135
AluI AGCT 7 cut(s) 46, 182, 265, 275, 728, 793, 1135
Alw21I GWGCWC 1 cut(s) 739
Alw26I GTCTC 5 cut(s) 277, 300, 395, 410, 837
AlwI GGATC 3 cut(s) 157, 703, 964
Ama87I CYCGRG 3 cut(s) 280, 676, 928
AoxI GGCC 1 cut(s) 894
ApeKI GCWGC 9 cut(s) 233, 265, 332, 335, 793, 796, 799, 871, 1132
ApoI RAATTY 2 cut(s) 149, 1140
Asp700I GAANNNNTTC 1 cut(s) 153
AspLEI GCGC 1 cut(s) 871
AspS9I GGNCC 2 cut(s) 767, 1102
AsuC2I CCSGG 3 cut(s) 383, 929, 930
AsuHPI GGTGA 2 cut(s) 362, 1099
AsuII TTCGAA 1 cut(s) 1138
AvaI CYCGRG 3 cut(s) 280, 676, 928
AvaII GGWCC 2 cut(s) 767, 1102
BalI TGGCCA 1 cut(s) 896
BanI GGYRCC 1 cut(s) 719
BbsI GAAGAC 2 cut(s) 402, 1207
Bbv12I GWGCWC 1 cut(s) 739
BbvI GCAGC 9 cut(s) 245, 252, 319, 322, 780, 808, 811, 858, 1144
BccI CCATC 5 cut(s) 55, 200, 361, 808, 1118
BceAI ACGGC 1 cut(s) 882
BciT130I CCWGG 3 cut(s) 203, 556, 572
BclI TGATCA 2 cut(s) 1095, 1206
BcnI CCSGG 3 cut(s) 383, 929, 930
BcoDI GTCTC 5 cut(s) 277, 300, 395, 410, 837
BcuI ACTAGT 1 cut(s) 889
BfaI CTAG 2 cut(s) 875, 890
BfmI CTRYAG 1 cut(s) 794
BfoI RGCGCY 1 cut(s) 872
BglI GCCNNNNNGGC 1 cut(s) 930
Bme1390I CCNGG 6 cut(s) 203, 383, 556, 572, 929, 930
Bme18I GGWCC 2 cut(s) 767, 1102
BmeT110I CYCGRG 3 cut(s) 280, 676, 928
BmgBI CACGTC 1 cut(s) 400
BmgT120I GGNCC 2 cut(s) 767, 1102
BmiI GGNNCC 2 cut(s) 721, 769
BmrFI CCNGG 6 cut(s) 203, 383, 556, 572, 929, 930
BmsI GCATC 2 cut(s) 847, 1287
BpiI GAAGAC 2 cut(s) 402, 1207
Bpu14I TTCGAA 1 cut(s) 1138
BpuMI CCSGG 3 cut(s) 383, 929, 930
BsaAI YACGTR 2 cut(s) 547, 1167
BsaI GGTCTC 1 cut(s) 300
BsaJI CCNNGG 5 cut(s) 430, 615, 771, 927, 928
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 5 cut(s) 61, 345, 693, 764, 932
Bse118I RCCGGY 1 cut(s) 686
Bse1I ACTGG 4 cut(s) 55, 448, 958, 979
Bse3DI GCAATG 3 cut(s) 601, 994, 1188
BseBI CCWGG 3 cut(s) 203, 556, 572
BseDI CCNNGG 5 cut(s) 430, 615, 771, 927, 928
BseGI GGATG 1 cut(s) 211
BseLI CCNNNNNNNGG 5 cut(s) 61, 345, 693, 764, 932
BseMI GCAATG 3 cut(s) 601, 994, 1188
BseMII CTCAG 4 cut(s) 9, 482, 934, 1017
BseNI ACTGG 4 cut(s) 55, 448, 958, 979
BseRI GAGGAG 1 cut(s) 309
BseXI GCAGC 9 cut(s) 245, 252, 319, 322, 780, 808, 811, 858, 1144
BseYI CCCAGC 1 cut(s) 851
BsgI GTGCAG 1 cut(s) 1197
Bsh1236I CGCG 2 cut(s) 230, 1154
BshFI GGCC 1 cut(s) 896
BshNI GGYRCC 1 cut(s) 719
BsiHKAI GWGCWC 1 cut(s) 739
BsiHKCI CYCGRG 3 cut(s) 280, 676, 928
BsiSI CCGG 6 cut(s) 138, 383, 687, 723, 915, 929
BslFI GGGAC 1 cut(s) 753
BslI CCNNNNNNNGG 5 cut(s) 61, 345, 693, 764, 932
BsmAI GTCTC 5 cut(s) 277, 300, 395, 410, 837
BsmFI GGGAC 1 cut(s) 753
BsnI GGCC 1 cut(s) 896
Bso31I GGTCTC 1 cut(s) 300
BsoBI CYCGRG 3 cut(s) 280, 676, 928
Bsp119I TTCGAA 1 cut(s) 1138
Bsp1286I GDGCHC 1 cut(s) 739
Bsp143I GATC 7 cut(s) 162, 658, 708, 956, 1095, 1206, 1290
Bsp19I CCATGG 1 cut(s) 771
Bsp68I TCGCGA 1 cut(s) 1154
BspACI CCGC 5 cut(s) 116, 230, 900, 933, 1276
BspANI GGCC 1 cut(s) 896
BspCNI CTCAG 4 cut(s) 10, 483, 933, 1016
BspFNI CGCG 2 cut(s) 230, 1154
BspLI GGNNCC 2 cut(s) 721, 769
BspMAI CTGCAG 1 cut(s) 798
BspPI GGATC 3 cut(s) 157, 703, 964
BspT104I TTCGAA 1 cut(s) 1138
BspT107I GGYRCC 1 cut(s) 719
BspTNI GGTCTC 1 cut(s) 300
BsrDI GCAATG 3 cut(s) 601, 994, 1188
BsrFI RCCGGY 1 cut(s) 686
BsrI ACTGG 4 cut(s) 55, 448, 958, 979
BssAI RCCGGY 1 cut(s) 686
BssECI CCNNGG 5 cut(s) 430, 615, 771, 927, 928
BssMI GATC 7 cut(s) 162, 658, 708, 956, 1095, 1206, 1290
BssT1I CCWWGG 3 cut(s) 430, 615, 771
Bst2UI CCWGG 3 cut(s) 203, 556, 572
Bst6I CTCTTC 3 cut(s) 298, 327, 699
BstBAI YACGTR 2 cut(s) 547, 1167
BstBI TTCGAA 1 cut(s) 1138
BstC8I GCNNGC 3 cut(s) 379, 688, 862
BstDEI CTNAG 4 cut(s) 18, 491, 920, 1003
BstDSI CCRYGG 1 cut(s) 771
BstF5I GGATG 1 cut(s) 211
BstFNI CGCG 2 cut(s) 230, 1154
BstH2I RGCGCY 1 cut(s) 872
BstHHI GCGC 1 cut(s) 871
BstKTI GATC 7 cut(s) 165, 661, 711, 959, 1098, 1209, 1293
BstMAI GTCTC 5 cut(s) 277, 300, 395, 410, 837
BstMBI GATC 7 cut(s) 162, 658, 708, 956, 1095, 1206, 1290
BstMWI GCNNNNNNNGC 9 cut(s) 134, 734, 799, 861, 866, 930, 984, 1004, 1132
BstNI CCWGG 3 cut(s) 203, 556, 572
BstNSI RCATGY 3 cut(s) 125, 146, 1265
BstSCI CCNGG 6 cut(s) 201, 381, 554, 570, 927, 928
BstSFI CTRYAG 1 cut(s) 794
BstUI CGCG 2 cut(s) 230, 1154
BstV1I GCAGC 9 cut(s) 245, 252, 319, 322, 780, 808, 811, 858, 1144
BstV2I GAAGAC 2 cut(s) 402, 1207
BstX2I RGATCY 2 cut(s) 162, 708
BstYI RGATCY 2 cut(s) 162, 708
BsuRI GGCC 1 cut(s) 896
BtgI CCRYGG 1 cut(s) 771
BtgZI GCGATG 2 cut(s) 211, 870
BtrI CACGTC 1 cut(s) 400
BtsCI GGATG 1 cut(s) 211
BtsI GCAGTG 2 cut(s) 985, 1180
BtsIMutI CAGTG 4 cut(s) 951, 985, 1180, 1216
BtuMI TCGCGA 1 cut(s) 1154
Cac8I GCNNGC 3 cut(s) 379, 688, 862
CfoI GCGC 1 cut(s) 871
Cfr10I RCCGGY 1 cut(s) 686
Cfr13I GGNCC 2 cut(s) 767, 1102
Cfr9I CCCGGG 1 cut(s) 928
CseI GACGC 1 cut(s) 1210
CsiI ACCWGGT 1 cut(s) 570
Csp6I GTAC 2 cut(s) 887, 1168
CviQI GTAC 2 cut(s) 887, 1168
DdeI CTNAG 4 cut(s) 18, 491, 920, 1003
DpnI GATC 7 cut(s) 164, 660, 710, 958, 1097, 1208, 1292
DpnII GATC 7 cut(s) 162, 658, 708, 956, 1095, 1206, 1290
EaeI YGGCCR 1 cut(s) 894
Eam1104I CTCTTC 3 cut(s) 298, 327, 699
EarI CTCTTC 3 cut(s) 298, 327, 699
Eco130I CCWWGG 3 cut(s) 430, 615, 771
Eco31I GGTCTC 1 cut(s) 300
Eco32I GATATC 1 cut(s) 40
Eco47I GGWCC 2 cut(s) 767, 1102
Eco57I CTGAAG 2 cut(s) 188, 773
Eco88I CYCGRG 3 cut(s) 280, 676, 928
EcoO109I RGGNCCY 1 cut(s) 1102
EcoRII CCWGG 3 cut(s) 201, 554, 570
EcoRV GATATC 1 cut(s) 40
EcoT14I CCWWGG 3 cut(s) 430, 615, 771
ErhI CCWWGG 3 cut(s) 430, 615, 771
FalI AAGNNNNNCTT 2 cut(s) 696, 728
FaqI GGGAC 1 cut(s) 753
FauI CCCGC 1 cut(s) 123
FbaI TGATCA 2 cut(s) 1095, 1206
FokI GGATG 1 cut(s) 218
FspBI CTAG 2 cut(s) 875, 890
GlaI GCGC 1 cut(s) 870
GsaI CCCAGC 1 cut(s) 855
HaeII RGCGCY 1 cut(s) 872
HaeIII GGCC 1 cut(s) 896
HapII CCGG 6 cut(s) 138, 383, 687, 723, 915, 929
HgaI GACGC 1 cut(s) 1210
HhaI GCGC 1 cut(s) 871
Hin6I GCGC 1 cut(s) 869
HinP1I GCGC 1 cut(s) 869
HinfI GANTC 9 cut(s) 5, 70, 285, 316, 403, 476, 845, 918, 1013
HpaII CCGG 6 cut(s) 138, 383, 687, 723, 915, 929
HphI GGTGA 2 cut(s) 362, 1099
Hpy166II GTNNAC 2 cut(s) 625, 634
Hpy188I TCNGA 2 cut(s) 19, 294
Hpy188III TCNNGA 8 cut(s) 158, 166, 392, 656, 676, 732, 1153, 1282
Hpy8I GTNNAC 2 cut(s) 625, 634
HpyAV CCTTC 4 cut(s) 490, 511, 722, 797
HpyCH4IV ACGT 4 cut(s) 399, 504, 546, 1166
HpyF10VI GCNNNNNNNGC 9 cut(s) 134, 734, 799, 861, 866, 930, 984, 1004, 1132
HpyF3I CTNAG 4 cut(s) 18, 491, 920, 1003
HpySE526I ACGT 4 cut(s) 399, 504, 546, 1166
HspAI GCGC 1 cut(s) 869
KroI GCCGGC 1 cut(s) 686
KroNI GCCGGC 1 cut(s) 688
Ksp22I TGATCA 2 cut(s) 1095, 1206
Kzo9I GATC 7 cut(s) 162, 658, 708, 956, 1095, 1206, 1290
LmnI GCTCC 4 cut(s) 87, 262, 725, 984
Lsp1109I GCAGC 9 cut(s) 245, 252, 319, 322, 780, 808, 811, 858, 1144
LweI GCATC 2 cut(s) 847, 1287
MabI ACCWGGT 1 cut(s) 570
MaeI CTAG 2 cut(s) 875, 890
MaeII ACGT 4 cut(s) 399, 504, 546, 1166
MaeIII GTNAC 1 cut(s) 400
MalI GATC 7 cut(s) 164, 660, 710, 958, 1097, 1208, 1292
MboI GATC 7 cut(s) 162, 658, 708, 956, 1095, 1206, 1290
MboII GAAGA 8 cut(s) 314, 315, 407, 533, 571, 664, 716, 1207
MfeI CAATTG 1 cut(s) 1248
MflI RGATCY 2 cut(s) 162, 708
MhlI GDGCHC 1 cut(s) 739
MlsI TGGCCA 1 cut(s) 896
MluCI AATT 6 cut(s) 149, 532, 699, 1114, 1140, 1248
MluNI TGGCCA 1 cut(s) 896
MlyI GAGTC 6 cut(s) 279, 310, 397, 839, 912, 1022
MmeI TCCRAC 1 cut(s) 272
Mox20I TGGCCA 1 cut(s) 896
MroNI GCCGGC 1 cut(s) 686
MroXI GAANNNNTTC 1 cut(s) 153
MscI TGGCCA 1 cut(s) 896
MseI TTAA 2 cut(s) 438, 462
MslI CAYNNNNRTG 1 cut(s) 432
Msp20I TGGCCA 1 cut(s) 896
MspA1I CMGCKG 1 cut(s) 793
MspI CCGG 6 cut(s) 138, 383, 687, 723, 915, 929
MspR9I CCNGG 6 cut(s) 203, 383, 556, 572, 929, 930
MunI CAATTG 1 cut(s) 1248
MvaI CCWGG 3 cut(s) 203, 556, 572
MvnI CGCG 2 cut(s) 230, 1154
MwoI GCNNNNNNNGC 9 cut(s) 134, 734, 799, 861, 866, 930, 984, 1004, 1132
NaeI GCCGGC 1 cut(s) 688
NciI CCSGG 3 cut(s) 383, 929, 930
NcoI CCATGG 1 cut(s) 771
NdeII GATC 7 cut(s) 162, 658, 708, 956, 1095, 1206, 1290
NgoMIV GCCGGC 1 cut(s) 686
NlaIV GGNNCC 2 cut(s) 721, 769
NmuCI GTSAC 1 cut(s) 400
NruI TCGCGA 1 cut(s) 1154
NspI RCATGY 3 cut(s) 125, 146, 1265
NspV TTCGAA 1 cut(s) 1138
OliI CACNNNNGTG 1 cut(s) 432
PciI ACATGT 2 cut(s) 121, 142
PdiI GCCGGC 1 cut(s) 688
PdmI GAANNNNTTC 1 cut(s) 153
PfeI GAWTC 3 cut(s) 5, 70, 476
PflFI GACNNNGTC 1 cut(s) 288
PflMI CCANNNNNTGG 1 cut(s) 61
PleI GAGTC 6 cut(s) 279, 310, 397, 839, 912, 1021
PpsI GAGTC 6 cut(s) 279, 310, 397, 839, 912, 1021
Ppu21I YACGTR 2 cut(s) 547, 1167
PpuMI RGGWCCY 1 cut(s) 1102
PscI ACATGT 2 cut(s) 121, 142
Psp1406I AACGTT 1 cut(s) 504
Psp5II RGGWCCY 1 cut(s) 1102
Psp6I CCWGG 3 cut(s) 201, 554, 570
PspFI CCCAGC 1 cut(s) 851
PspGI CCWGG 3 cut(s) 201, 554, 570
PspN4I GGNNCC 2 cut(s) 721, 769
PspPI GGNCC 2 cut(s) 767, 1102
PspPPI RGGWCCY 1 cut(s) 1102
PstI CTGCAG 1 cut(s) 798
PsuI RGATCY 2 cut(s) 162, 708
PsyI GACNNNGTC 1 cut(s) 288
PvuII CAGCTG 1 cut(s) 793
RruI TCGCGA 1 cut(s) 1154
RsaI GTAC 2 cut(s) 888, 1169
RsaNI GTAC 2 cut(s) 887, 1168
RseI CAYNNNNRTG 1 cut(s) 432
SaqAI TTAA 2 cut(s) 438, 462
Sau3AI GATC 7 cut(s) 162, 658, 708, 956, 1095, 1206, 1290
Sau96I GGNCC 2 cut(s) 767, 1102
SchI GAGTC 6 cut(s) 279, 310, 397, 839, 912, 1022
ScrFI CCNGG 6 cut(s) 203, 383, 556, 572, 929, 930
SduI GDGCHC 1 cut(s) 739
SexAI ACCWGGT 1 cut(s) 570
SfaNI GCATC 2 cut(s) 847, 1287
SfcI CTRYAG 1 cut(s) 794
SfuI TTCGAA 1 cut(s) 1138
SinI GGWCC 2 cut(s) 767, 1102
SmaI CCCGGG 1 cut(s) 930
SmiMI CAYNNNNRTG 1 cut(s) 432
SpeI ACTAGT 1 cut(s) 889
Sse9I AATT 6 cut(s) 149, 532, 699, 1114, 1140, 1248
SsiI CCGC 5 cut(s) 116, 230, 900, 933, 1276
SspMI CTAG 2 cut(s) 875, 890
StyD4I CCNGG 6 cut(s) 201, 381, 554, 570, 927, 928
StyI CCWWGG 3 cut(s) 430, 615, 771
TaiI ACGT 4 cut(s) 402, 507, 549, 1169
TaqI TCGA 7 cut(s) 8, 147, 393, 731, 1067, 1138, 1289
TaqII GACCGA 1 cut(s) 469
TasI AATT 6 cut(s) 149, 532, 699, 1114, 1140, 1248
TatI WGTACW 1 cut(s) 886
TauI GCSGC 3 cut(s) 233, 936, 1278
TfiI GAWTC 3 cut(s) 5, 70, 476
Tru1I TTAA 2 cut(s) 438, 462
Tru9I TTAA 2 cut(s) 438, 462
TscAI CASTG 4 cut(s) 958, 985, 1180, 1216
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 9 cut(s) 233, 265, 332, 335, 793, 796, 799, 871, 1132
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 5 cut(s) 207, 212, 314, 530, 1005
TspGWI ACGGA 2 cut(s) 1006, 1259
TspMI CCCGGG 1 cut(s) 928
TspRI CASTG 4 cut(s) 958, 985, 1180, 1216
Tth111I GACNNNGTC 1 cut(s) 288
Van91I CCANNNNNTGG 1 cut(s) 61
VpaK11BI GGWCC 2 cut(s) 767, 1102
XapI RAATTY 2 cut(s) 149, 1140
XceI RCATGY 3 cut(s) 125, 146, 1265
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XmaI CCCGGG 1 cut(s) 928
XmnI GAANNNNTTC 1 cut(s) 153
XspI CTAG 2 cut(s) 875, 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.