Rh1AG385700

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0002292
Physical Location & Seq
Forward (+)
61648826 .. 61650974
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG385700.1

Sequence Viewer

Length: 1368 bp
ATGGAGCAAAGGCTGTTTGAGGCTTCTCAAAGTGGAAATATCCAACTTCTGCACCAGTTGCTTGCAGAGAATCCACTCCTACTCCATAGTCTTGCTCTAGATTCTGCAGAGAACCCGGTACATGTTGCTTCACTTGCTGACCATGTTGGCTTTGTTAAGGAGATTCTAAGGTTGAAACCTGCTTTGGCCAAAGAAATGAACCCAGATGGCTTGAGCCCTATGCATATAGCATCTGCCAACGGATATTTGGAGATTGTAGGGGAGCTTCTGAAAGTTGATCTAAGACTGTGCCGGCTAAAGGGAAGAGATCAATGGACTCCTCTTCATCATGCTGCAAGGAGAGGAAGGGTAGATATTGTTAGAGAAATAGTCTTGGCTTGTCCGGAAAGTGTTGAAGATGTGACAATACAAGGGGAAACTGCTTTGCACCTTGCTGTTAAGAACAGTCAGGTTGGAGCTGTTGAAGTTTTGGTGAAATCGGTCACAGAAATGAGGAAGATGAACATCCTGGATTTGAAGGATAAGCATGGCAATACAGTTCTTCACCTAGCAACATGGAAAAAACAACAGCAGGTGGTGGAATGGTTAATTGGAATTGGGGATAGTACCCCAGGCGCAATGGAAGTGAACAACGTAAACCAGAGTGGTCTCACTGCTCTTGATCTTCTACTACTTTTCCCAAGTGAAGCTGGTGATCGAGAAATCTACGACACCCTTAGTGGTGCTGGAGCTTTGAGAGCACAAGACATGGTCCATTCTGCTATACCTTCATTTGACTCTGATAACCAAACCTTCTTGAACTGCCCCGTGGCATCAGGGACACATCAGTTGCAGCATCCAAATGATTTCATCCAATACTTCAAGTTCAAAAAGGGCAGAGATTCTCCTAGTGATGCGCGCACCGCGCTGTTAGTTGTAGCCGTATTGGTGACTACAGCGACATTTCAAGTGGGACTAAGCCCTCCAACCGGTGTTTGGCAGGACACCGAGCTGAAAAATAATGGGACTAGCAGTGAACCAACACACAATGCAGGAACGTCTATATTGGGGTCTTATAACGCAGCTGCATTTGTGGTTTTCGTGGCTTTCAACTCCATTGGTTTTTCGGTTTCTCTTTACATGATCAACATTCTCACAAGCAATTTCCCTTTGCAGTTGGAGCTTCAGATCTGTATCATTGCCATGTATTGTACATACAACACAGCGATGATTAGCATTGCACCGAATAACACAAGAGTCTTCATTATTGTATTTACTTCGGTTTTGCCTACTGCGGTATCACTTGCTGCCAAATCAGTTAGGCAACTTGTTATGAGGCTTGGAGAACCTCTGGTAAATTCAGCGCGGAGATTCATTCATTCGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

455

Amino Acids

49.73

Weight (kDa)

6.2

Isoelectric Point (pI)

36.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 5 - 91 1.3e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 41 - 124 3.4e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 80 - 160 4.1e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 134 - 197 1.4e-09 Ankyrin repeats (3 copies)
PGG PF13962 298 - 403 1.4e-16 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1056
AarI CACCTGC 1 cut(s) 562
Acc36I ACCTGC 2 cut(s) 187, 562
AccII CGCG 3 cut(s) 898, 905, 1345
AccIII TCCGGA 1 cut(s) 382
AciI CCGC 3 cut(s) 903, 1274, 1345
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 1 cut(s) 1336
AcuI CTGAAG 1 cut(s) 1148
AfaI GTAC 3 cut(s) 120, 607, 1192
AfiI CCNNNNNNNGG 3 cut(s) 298, 968, 975
AflIII ACRYGT 1 cut(s) 121
AgeI ACCGGT 1 cut(s) 968
AgsI TTSAA 9 cut(s) 175, 395, 464, 517, 799, 862, 868, 947, 1090
AjnI CCWGG 2 cut(s) 507, 610
AjuI GAANNNNNNNTTGG 4 cut(s) 167, 199, 573, 605
AluBI AGCT 7 cut(s) 265, 458, 689, 731, 991, 1064, 1162
AluI AGCT 7 cut(s) 265, 458, 689, 731, 991, 1064, 1162
Alw21I GWGCWC 1 cut(s) 742
Alw26I GTCTC 1 cut(s) 653
Aor13HI TCCGGA 1 cut(s) 382
AoxI GGCC 1 cut(s) 186
ApeKI GCWGC 5 cut(s) 332, 832, 1061, 1064, 1286
ApoI RAATTY 1 cut(s) 1336
AsiGI ACCGGT 1 cut(s) 968
AspLEI GCGC 5 cut(s) 617, 898, 900, 907, 1345
AspS9I GGNCC 1 cut(s) 751
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 4 cut(s) 484, 536, 704, 940
AsuII TTCGAA 1 cut(s) 1361
AvaII GGWCC 1 cut(s) 751
BaeI ACNNNNGTAYC 2 cut(s) 1260, 1293
BalI TGGCCA 1 cut(s) 188
BanII GRGCYC 1 cut(s) 218
BarI GAAGNNNNNNTAC 2 cut(s) 249, 281
BbsI GAAGAC 1 cut(s) 1231
Bbv12I GWGCWC 1 cut(s) 742
BbvI GCAGC 5 cut(s) 319, 844, 1051, 1073, 1273
BccI CCATC 1 cut(s) 200
BceAI ACGGC 1 cut(s) 905
BciT130I CCWGG 2 cut(s) 509, 612
BclI TGATCA 1 cut(s) 1122
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 1 cut(s) 653
BfaI CTAG 5 cut(s) 98, 548, 888, 1008, 1366
BfmI CTRYAG 2 cut(s) 105, 933
BfuAI ACCTGC 2 cut(s) 187, 562
BglII AGATCT 1 cut(s) 1167
BisI GCNGC 5 cut(s) 333, 833, 1062, 1065, 1287
BlsI GCNGC 5 cut(s) 334, 834, 1063, 1066, 1288
Bme1390I CCNGG 3 cut(s) 116, 509, 612
Bme18I GGWCC 1 cut(s) 751
BmgT120I GGNCC 1 cut(s) 751
BmrFI CCNGG 3 cut(s) 116, 509, 612
BmsI GCATC 4 cut(s) 239, 821, 844, 883
BpiI GAAGAC 1 cut(s) 1231
BpmI CTGGAG 1 cut(s) 747
Bpu14I TTCGAA 1 cut(s) 1361
BpuEI CTTGAG 1 cut(s) 232
BpuMI CCSGG 1 cut(s) 116
BsaBI GATNNNNATC 2 cut(s) 503, 1172
BsaI GGTCTC 1 cut(s) 653
BsaJI CCNNGG 2 cut(s) 610, 807
BsaWI WCCGGW 2 cut(s) 382, 968
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 3 cut(s) 298, 968, 975
Bse118I RCCGGY 2 cut(s) 291, 968
Bse1I ACTGG 1 cut(s) 55
Bse3DI GCAATG 3 cut(s) 624, 1176, 1215
Bse8I GATNNNNATC 2 cut(s) 503, 1172
BseAI TCCGGA 1 cut(s) 382
BseBI CCWGG 2 cut(s) 509, 612
BseDI CCNNGG 2 cut(s) 610, 807
BseGI GGATG 3 cut(s) 504, 835, 849
BseJI GATNNNNATC 2 cut(s) 503, 1172
BseLI CCNNNNNNNGG 3 cut(s) 298, 968, 975
BseMI GCAATG 3 cut(s) 624, 1176, 1215
BseNI ACTGG 1 cut(s) 55
BsePI GCGCGC 1 cut(s) 896
BseRI GAGGAG 1 cut(s) 309
BseXI GCAGC 5 cut(s) 319, 844, 1051, 1073, 1273
BsgI GTGCAG 1 cut(s) 35
Bsh1236I CGCG 3 cut(s) 898, 905, 1345
BshFI GGCC 1 cut(s) 188
BshTI ACCGGT 1 cut(s) 968
BsiHKAI GWGCWC 1 cut(s) 742
BsiSI CCGG 4 cut(s) 116, 292, 383, 969
BslFI GGGAC 3 cut(s) 832, 966, 1018
BslI CCNNNNNNNGG 3 cut(s) 298, 968, 975
BsmAI GTCTC 1 cut(s) 653
BsmFI GGGAC 3 cut(s) 832, 966, 1018
BsnI GGCC 1 cut(s) 188
Bso31I GGTCTC 1 cut(s) 653
Bsp119I TTCGAA 1 cut(s) 1361
Bsp1286I GDGCHC 2 cut(s) 218, 742
Bsp13I TCCGGA 1 cut(s) 382
Bsp1407I TGTACA 1 cut(s) 1190
Bsp143I GATC 6 cut(s) 277, 307, 661, 694, 1122, 1167
BspACI CCGC 3 cut(s) 903, 1274, 1345
BspANI GGCC 1 cut(s) 188
BspEI TCCGGA 1 cut(s) 382
BspFNI CGCG 3 cut(s) 898, 905, 1345
BspMAI CTGCAG 1 cut(s) 109
BspMI ACCTGC 2 cut(s) 187, 562
BspT104I TTCGAA 1 cut(s) 1361
BspTNI GGTCTC 1 cut(s) 653
BsrDI GCAATG 3 cut(s) 624, 1176, 1215
BsrFI RCCGGY 2 cut(s) 291, 968
BsrGI TGTACA 1 cut(s) 1190
BsrI ACTGG 1 cut(s) 55
BssAI RCCGGY 2 cut(s) 291, 968
BssECI CCNNGG 2 cut(s) 610, 807
BssHII GCGCGC 1 cut(s) 896
BssMI GATC 6 cut(s) 277, 307, 661, 694, 1122, 1167
Bst2UI CCWGG 2 cut(s) 509, 612
Bst4CI ACNGT 3 cut(s) 288, 446, 538
Bst6I CTCTTC 2 cut(s) 298, 327
BstAPI GCANNNNNTGC 1 cut(s) 58
BstAUI TGTACA 1 cut(s) 1190
BstBI TTCGAA 1 cut(s) 1361
BstC8I GCNNGC 3 cut(s) 63, 293, 898
BstDEI CTNAG 4 cut(s) 167, 281, 716, 956
BstDSI CCRYGG 1 cut(s) 807
BstF5I GGATG 3 cut(s) 504, 835, 849
BstFNI CGCG 3 cut(s) 898, 905, 1345
BstHHI GCGC 5 cut(s) 617, 898, 900, 907, 1345
BstKTI GATC 6 cut(s) 280, 310, 664, 697, 1125, 1170
BstMAI GTCTC 1 cut(s) 653
BstMBI GATC 6 cut(s) 277, 307, 661, 694, 1122, 1167
BstMWI GCNNNNNNNGC 6 cut(s) 58, 134, 737, 902, 904, 1159
BstNI CCWGG 2 cut(s) 509, 612
BstNSI RCATGY 1 cut(s) 125
BstSCI CCNGG 3 cut(s) 114, 507, 610
BstSFI CTRYAG 2 cut(s) 105, 933
BstUI CGCG 3 cut(s) 898, 905, 1345
BstV1I GCAGC 5 cut(s) 319, 844, 1051, 1073, 1273
BstV2I GAAGAC 1 cut(s) 1231
BstX2I RGATCY 1 cut(s) 1167
BstYI RGATCY 1 cut(s) 1167
BsuRI GGCC 1 cut(s) 188
BtgI CCRYGG 1 cut(s) 807
BtgZI GCGATG 1 cut(s) 1220
BtsCI GGATG 3 cut(s) 504, 835, 849
BtsI GCAGTG 2 cut(s) 651, 1018
BtsIMutI CAGTG 2 cut(s) 651, 1018
BveI ACCTGC 2 cut(s) 187, 562
Cac8I GCNNGC 3 cut(s) 63, 293, 898
CfoI GCGC 5 cut(s) 617, 898, 900, 907, 1345
Cfr10I RCCGGY 2 cut(s) 291, 968
Cfr13I GGNCC 1 cut(s) 751
Csp6I GTAC 3 cut(s) 119, 606, 1191
CspAI ACCGGT 1 cut(s) 968
CviAII CATG 8 cut(s) 122, 143, 329, 527, 555, 748, 1120, 1183
CviQI GTAC 3 cut(s) 119, 606, 1191
DdeI CTNAG 4 cut(s) 167, 281, 716, 956
DpnI GATC 6 cut(s) 279, 309, 663, 696, 1124, 1169
DpnII GATC 6 cut(s) 277, 307, 661, 694, 1122, 1167
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 2 cut(s) 298, 327
EarI CTCTTC 2 cut(s) 298, 327
Eco24I GRGCYC 1 cut(s) 218
Eco31I GGTCTC 1 cut(s) 653
Eco47I GGWCC 1 cut(s) 751
Eco57I CTGAAG 1 cut(s) 1148
EcoRII CCWGG 2 cut(s) 507, 610
EcoT22I ATGCAT 1 cut(s) 225
EcoT38I GRGCYC 1 cut(s) 218
FaeI CATG 8 cut(s) 125, 146, 332, 530, 558, 751, 1123, 1186
FaqI GGGAC 3 cut(s) 832, 966, 1018
FatI CATG 8 cut(s) 121, 142, 328, 526, 554, 747, 1119, 1182
FbaI TGATCA 1 cut(s) 1122
Fnu4HI GCNGC 5 cut(s) 333, 833, 1062, 1065, 1287
FokI GGATG 3 cut(s) 491, 822, 836
FriOI GRGCYC 1 cut(s) 218
Fsp4HI GCNGC 5 cut(s) 333, 833, 1062, 1065, 1287
FspBI CTAG 5 cut(s) 98, 548, 888, 1008, 1366
GlaI GCGC 5 cut(s) 616, 897, 899, 906, 1344
GluI GCNGC 5 cut(s) 333, 833, 1062, 1065, 1287
GsuI CTGGAG 1 cut(s) 747
HaeIII GGCC 1 cut(s) 188
HapII CCGG 4 cut(s) 116, 292, 383, 969
HhaI GCGC 5 cut(s) 617, 898, 900, 907, 1345
Hin1II CATG 8 cut(s) 125, 146, 332, 530, 558, 751, 1123, 1186
Hin6I GCGC 5 cut(s) 615, 896, 898, 905, 1343
HinP1I GCGC 5 cut(s) 615, 896, 898, 905, 1343
HinfI GANTC 8 cut(s) 70, 101, 163, 316, 776, 881, 1236, 1350
HpaII CCGG 4 cut(s) 116, 292, 383, 969
HphI GGTGA 4 cut(s) 484, 536, 704, 940
Hpy166II GTNNAC 3 cut(s) 628, 637, 1016
Hpy188I TCNGA 3 cut(s) 270, 781, 1167
Hpy188III TCNNGA 5 cut(s) 98, 383, 659, 698, 796
Hpy8I GTNNAC 3 cut(s) 628, 637, 1016
HpyAV CCTTC 4 cut(s) 339, 511, 777, 802
HpyCH4III ACNGT 3 cut(s) 288, 446, 538
HpyCH4IV ACGT 2 cut(s) 633, 1037
HpyF10VI GCNNNNNNNGC 6 cut(s) 58, 134, 737, 902, 904, 1159
HpyF3I CTNAG 4 cut(s) 167, 281, 716, 956
HpySE526I ACGT 2 cut(s) 633, 1037
Hsp92II CATG 8 cut(s) 125, 146, 332, 530, 558, 751, 1123, 1186
HspAI GCGC 5 cut(s) 615, 896, 898, 905, 1343
Kpn2I TCCGGA 1 cut(s) 382
KroI GCCGGC 1 cut(s) 291
KroNI GCCGGC 1 cut(s) 293
Ksp22I TGATCA 1 cut(s) 1122
Kzo9I GATC 6 cut(s) 277, 307, 661, 694, 1122, 1167
LmnI GCTCC 5 cut(s) 4, 262, 455, 728, 1159
Lsp1109I GCAGC 5 cut(s) 319, 844, 1051, 1073, 1273
LweI GCATC 4 cut(s) 239, 821, 844, 883
MaeI CTAG 5 cut(s) 98, 548, 888, 1008, 1366
MaeII ACGT 2 cut(s) 633, 1037
MaeIII GTNAC 3 cut(s) 400, 481, 928
MalI GATC 6 cut(s) 279, 309, 663, 696, 1124, 1169
MboI GATC 6 cut(s) 277, 307, 661, 694, 1122, 1167
MboII GAAGA 7 cut(s) 314, 315, 407, 508, 533, 656, 1231
MflI RGATCY 1 cut(s) 1167
MhlI GDGCHC 2 cut(s) 218, 742
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 4 cut(s) 588, 594, 1141, 1336
MluNI TGGCCA 1 cut(s) 188
MlyI GAGTC 3 cut(s) 310, 770, 1245
MmeI TCCRAC 4 cut(s) 67, 433, 989, 1137
MnlI CCTC 7 cut(s) 13, 330, 335, 486, 972, 1308, 1338
Mox20I TGGCCA 1 cut(s) 188
Mph1103I ATGCAT 1 cut(s) 225
MroI TCCGGA 1 cut(s) 382
MroNI GCCGGC 1 cut(s) 291
MscI TGGCCA 1 cut(s) 188
MseI TTAA 3 cut(s) 156, 438, 587
MslI CAYNNNNRTG 4 cut(s) 488, 840, 1181, 1205
Msp20I TGGCCA 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 1064
MspI CCGG 4 cut(s) 116, 292, 383, 969
MspR9I CCNGG 3 cut(s) 116, 509, 612
MvaI CCWGG 2 cut(s) 509, 612
MvnI CGCG 3 cut(s) 898, 905, 1345
MwoI GCNNNNNNNGC 6 cut(s) 58, 134, 737, 902, 904, 1159
NaeI GCCGGC 1 cut(s) 293
NciI CCSGG 1 cut(s) 116
NdeII GATC 6 cut(s) 277, 307, 661, 694, 1122, 1167
NgoMIV GCCGGC 1 cut(s) 291
NlaIII CATG 8 cut(s) 125, 146, 332, 530, 558, 751, 1123, 1186
NmuCI GTSAC 3 cut(s) 400, 481, 928
NsiI ATGCAT 1 cut(s) 225
NspI RCATGY 1 cut(s) 125
NspV TTCGAA 1 cut(s) 1361
PaqCI CACCTGC 1 cut(s) 562
PauI GCGCGC 1 cut(s) 896
PciI ACATGT 1 cut(s) 121
PdiI GCCGGC 1 cut(s) 293
PfeI GAWTC 5 cut(s) 70, 101, 163, 881, 1350
PflFI GACNNNGTC 1 cut(s) 749
PfoI TCCNGGA 1 cut(s) 507
PinAI ACCGGT 1 cut(s) 968
PkrI GCNGC 5 cut(s) 334, 834, 1063, 1066, 1288
PleI GAGTC 3 cut(s) 310, 770, 1244
PpsI GAGTC 3 cut(s) 310, 770, 1244
PscI ACATGT 1 cut(s) 121
PsiI TTATAA 1 cut(s) 1056
Psp6I CCWGG 2 cut(s) 507, 610
PspGI CCWGG 2 cut(s) 507, 610
PspPI GGNCC 1 cut(s) 751
PstI CTGCAG 1 cut(s) 109
PsuI RGATCY 1 cut(s) 1167
PsyI GACNNNGTC 1 cut(s) 749
PteI GCGCGC 1 cut(s) 896
PvuII CAGCTG 1 cut(s) 1064
RsaI GTAC 3 cut(s) 120, 607, 1192
RsaNI GTAC 3 cut(s) 119, 606, 1191
RseI CAYNNNNRTG 4 cut(s) 488, 840, 1181, 1205
SaqAI TTAA 3 cut(s) 156, 438, 587
SatI GCNGC 5 cut(s) 333, 833, 1062, 1065, 1287
Sau3AI GATC 6 cut(s) 277, 307, 661, 694, 1122, 1167
Sau96I GGNCC 1 cut(s) 751
SchI GAGTC 3 cut(s) 310, 770, 1245
ScrFI CCNGG 3 cut(s) 116, 509, 612
SduI GDGCHC 2 cut(s) 218, 742
SfaNI GCATC 4 cut(s) 239, 821, 844, 883
SfcI CTRYAG 2 cut(s) 105, 933
SfuI TTCGAA 1 cut(s) 1361
SinI GGWCC 1 cut(s) 751
SmiMI CAYNNNNRTG 4 cut(s) 488, 840, 1181, 1205
SmlI CTYRAG 1 cut(s) 211
SmoI CTYRAG 1 cut(s) 211
Sse9I AATT 4 cut(s) 588, 594, 1141, 1336
SsiI CCGC 3 cut(s) 903, 1274, 1345
SspMI CTAG 5 cut(s) 98, 548, 888, 1008, 1366
StyD4I CCNGG 3 cut(s) 114, 507, 610
TaaI ACNGT 3 cut(s) 288, 446, 538
TaiI ACGT 2 cut(s) 636, 1040
TaqI TCGA 2 cut(s) 697, 1361
TaqII GACCGA 1 cut(s) 469
TasI AATT 4 cut(s) 588, 594, 1141, 1336
TatI WGTACW 1 cut(s) 1190
TfiI GAWTC 5 cut(s) 70, 101, 163, 881, 1350
Tru1I TTAA 3 cut(s) 156, 438, 587
Tru9I TTAA 3 cut(s) 156, 438, 587
TscAI CASTG 2 cut(s) 658, 1018
TseFI GTSAC 3 cut(s) 400, 481, 928
TseI GCWGC 5 cut(s) 332, 832, 1061, 1064, 1286
Tsp45I GTSAC 3 cut(s) 400, 481, 928
TspDTI ATGAA 8 cut(s) 212, 314, 515, 759, 838, 1231, 1342, 1346
TspGWI ACGGA 1 cut(s) 255
TspRI CASTG 2 cut(s) 658, 1018
Tth111I GACNNNGTC 1 cut(s) 749
VpaK11BI GGWCC 1 cut(s) 751
XapI RAATTY 1 cut(s) 1336
XbaI TCTAGA 1 cut(s) 97
XceI RCATGY 1 cut(s) 125
XcmI CCANNNNNNNNNTGG 2 cut(s) 244, 972
XspI CTAG 5 cut(s) 98, 548, 888, 1008, 1366
Zsp2I ATGCAT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.