Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002292

Physical Location & Seq

Genomic Coordinates Reverse (-)

30598188 .. 30599660

Gene Structure 1473 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1230900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1395 bp

ATGGATGCAAGGCTGTTTGACGCTTCTCAGACGGGAGATGTCCAACTTCTGCATCGATTGCTGGCAGAGAATCCACTTCTACTCCATAGCCTTGCCCTAACTTCCACAGATAATCCTTTACATGTTGCTTCGATTGCTGGGCATGTCGACTTTGTCAAGGAGATTGTGAGGTTGAAACCAGATTATGTCGAAGAACTGAACCGGGATGGTTTTAGCCCCATGCATATTGCATCCGCCAATGGGTATTTGGAAATTGTAAGGGAGATGCTGAGGGTTGACCGGAGACTCTGCCAGCTAAACGGAAGAGATGAGTGGACTCCTCTCCACTATGCCGCTAGCAGGGGGAGGGTAGACGTAATTAGAGAAATGGTCTTGGCTTGTCCAGAAAGTGTTGAAGATGTGACCGTACAAGGGGAGACTGTTATTCACCTTGCTGTTAAGTGCAGCCAGTTTAAAGCAATTAAATTTTTGGTGGAAGAATTGGTTGCAGAAGCGAACAAGGTGTACATCTTGAACATGAAGGATAAGCATGGCGACACAGTTCTTCATCTAGCAACCTGGAAAAAACAACATCAGGTGGTGGAAGGATTAGTTGGCAGTGAAACAATTGCAGGAGCCTTGGAAGTAAATTCTGTAAACGATAGGGGTCTCACAGCTTTGGATCTGGTCTTCCCAAATGAAACCGGTGATTGGGAAATGGAGGCAATCCTTCGTGGTGCTGGAGCTTTGAGAGCAGGAGATATAGTCCACTCCGGTGTCCAATTCTCCAATTCTCATAGCCCTAACCATAACCACACGTCATCAGAGACTCATCAGTTGCAGCAGGAGCAGCAGGAGCAGCAGAAGACAAATAGGTGGGAATACTTCCAGTTCAAAAAGGGCAGAGACAGTCCTAGCGATGCACGTAACGCGCTGCTAGTAGTCGCTTCACTAGTGGCTACGGCCACCTTTCAAGTGGGACTCAACCCTCCAAATGGAATTTGGCAGGACAGTAATGATGTGAATAATAGTGGGACTGGCAGCAGCAATGAACCAGTACACTTGGCAGGAAGGTCCATCATGGGTTCTTATAGTGAAGTTTCATTCGTCATTTTTGTGGTTTTCAACTCGATTGGATTTTACTTTTCTCTTTACACCATGATCGTCCTCACAGCCAATTTCCCTCTCCAGTTGGAGCTTCAAATCTGCATCATAGCGGTATACACCACCTACAGCACTGCGGTTGTCAACACATTGCACCATCGTATAACACCGGGCTCTTTATCACCGTGTTTCACATCGGTTTTGCCCTTTTTGGTCGGACTTGCTGCCAAATGGGCTAGACACCCTGTCGCAATCTGGCTTACAAAACCTCTCGGAAAATGGATCCTTCGGTTCTTTGAATTAATTTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Ank_4 PGG

Protein Analysis

465

Amino Acids

51.7

Weight (kDa)

5.94

Isoelectric Point (pI)

32.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	5 - 90	1.9e-09	Ankyrin repeats (3 copies)
Ank_2	PF12796	100 - 160	6.5e-09	Ankyrin repeats (3 copies)
Ank_2	PF12796	137 - 199	3.5e-07	Ankyrin repeats (3 copies)
PGG	PF13962	300 - 387	1.1e-16	Domain of unknown function

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002292)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51160
fragaria_vesca	FvH4_7g26641 FvH4_7g26643 FvH4_7g26670
malus_domestica	MD01G1175400.v1.1 MD07G1241300.v1.1 MD07G1241500.v1.1 MD12G1230800.v1.1 MD12G1230900.v1.1 MD12G1231100.v1.1 MD12G1231200.v1.1
prunus_persica	Prupe.2G270400_v2.0.a1 Prupe.2G270500_v2.0.a1
pyrus_communis	pycom07g21780 pycom07g21790 pycom07g21800 pycom07g21860 pycom07g21970 pycom12g21410
rosa_chinensis	RchiOBHm_Chr1g0373271 RchiOBHm_Chr1g0373281
rosa_laevigata	RLG00000026830 RLG00000026831 RLG00000026832
rosa_multiflora	Rmu_co8287043.1_g000001 Rmu_sc0000170.1_g000005 Rmu_ssc0000435.1_g000001 Rmu_ssc0000435.1_g000002
rosa_rugosa	Rorug01G0376800 Rorug01G0376800
rosa_samantha	Rh1AG385600 Rh1AG385700 Rh1BG349800 Rh1BG349900 Rh1CG363000 Rh1CG363100 Rh1DG380500 Rh1DG380600 Rh1DG380700 Rh7DG228200
rosa_wichuraiana	Rw1G034920 Rw1G034930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	3 cut(s)	147, 351, 1200
AccII	CGCG	1 cut(s)	911
AciI	CCGC	4 cut(s)	234, 333, 1196, 1220
AclWI	GGATC	3 cut(s)	669, 1360, 1373
AcoI	YGGCCR	1 cut(s)	942
AcsI	RAATTY	3 cut(s)	464, 628, 978
AfaI	GTAC	3 cut(s)	408, 506, 1038
AfiI	CCNNNNNNNGG	5 cut(s)	240, 339, 411, 690, 974
AflIII	ACRYGT	2 cut(s)	121, 795
AgeI	ACCGGT	1 cut(s)	683
AgsI	TTSAA	8 cut(s)	175, 395, 514, 874, 953, 1105, 1181, 1382
AhdI	GACNNNNNGTC	1 cut(s)	1328
AhlI	ACTAGT	1 cut(s)	931
AjiI	CACGTC	1 cut(s)	798
AjnI	CCWGG	1 cut(s)	557
AjuI	GAANNNNNNNTTGG	2 cut(s)	576, 608
AleI	CACNNNNGTG	1 cut(s)	753
AluBI	AGCT	4 cut(s)	295, 656, 725, 1177
AluI	AGCT	4 cut(s)	295, 656, 725, 1177
Alw26I	GTCTC	5 cut(s)	277, 410, 653, 800, 879
AlwI	GGATC	3 cut(s)	669, 1360, 1373
AoxI	GGCC	1 cut(s)	942
ApeKI	GCWGC	8 cut(s)	444, 820, 829, 838, 913, 1020, 1023, 1307
ApoI	RAATTY	3 cut(s)	464, 628, 978
AseI	ATTAAT	1 cut(s)	1385
AsiGI	ACCGGT	1 cut(s)	683
AspLEI	GCGC	1 cut(s)	913
AspS9I	GGNCC	1 cut(s)	1053
AsuC2I	CCSGG	2 cut(s)	203, 1254
AsuHPI	GGTGA	3 cut(s)	419, 698, 1257
AsuNHI	GCTAGC	1 cut(s)	335
AvaII	GGWCC	1 cut(s)	1053
BamHI	GGATCC	1 cut(s)	1365
BanII	GRGCYC	1 cut(s)	1259
BbsI	GAAGAC	2 cut(s)	661, 851
BbvCI	CCTCAGC	1 cut(s)	269
BbvI	GCAGC	8 cut(s)	456, 832, 841, 850, 900, 1032, 1035, 1294
BccI	CCATC	3 cut(s)	200, 1064, 1248
BceAI	ACGGC	1 cut(s)	957
BciT130I	CCWGG	1 cut(s)	559
BcnI	CCSGG	2 cut(s)	203, 1254
BcoDI	GTCTC	5 cut(s)	277, 410, 653, 800, 879
BcuI	ACTAGT	1 cut(s)	931
BfaI	CTAG	6 cut(s)	336, 551, 894, 917, 932, 1320
BfmI	CTRYAG	1 cut(s)	1210
BglI	GCCNNNNNGGC	1 cut(s)	1316
BisI	GCNGC	9 cut(s)	333, 445, 821, 830, 839, 914, 1021, 1024, 1308
BlsI	GCNGC	9 cut(s)	334, 446, 822, 831, 840, 915, 1022, 1025, 1309
Bme1390I	CCNGG	3 cut(s)	203, 559, 1254
Bme18I	GGWCC	1 cut(s)	1053
BmeRI	GACNNNNNGTC	1 cut(s)	1328
BmgBI	CACGTC	1 cut(s)	798
BmgT120I	GGNCC	1 cut(s)	1053
BmiI	GGNNCC	2 cut(s)	616, 1367
BmrFI	CCNGG	3 cut(s)	203, 559, 1254
BmsI	GCATC	5 cut(s)	61, 239, 255, 889, 1197
BmtI	GCTAGC	1 cut(s)	339
BpiI	GAAGAC	2 cut(s)	661, 851
BpmI	CTGGAG	2 cut(s)	741, 1151
Bpu10I	CCTNAGC	1 cut(s)	269
BpuMI	CCSGG	2 cut(s)	203, 1254
Bsa29I	ATCGAT	1 cut(s)	55
BsaAI	YACGTR	1 cut(s)	905
BsaI	GGTCTC	1 cut(s)	653
BsaJI	CCNNGG	1 cut(s)	618
BsaWI	WCCGGW	3 cut(s)	279, 683, 752
BsaXI	ACNNNNNCTCC	4 cut(s)	66, 96, 729, 759
Bsc4I	CCNNNNNNNGG	5 cut(s)	240, 339, 411, 690, 974
Bse118I	RCCGGY	1 cut(s)	683
Bse1I	ACTGG	5 cut(s)	448, 868, 1021, 1034, 1168
Bse3DI	GCAATG	2 cut(s)	1033, 1232
BseBI	CCWGG	1 cut(s)	559
BseCI	ATCGAT	1 cut(s)	55
BseDI	CCNNGG	1 cut(s)	618
BseGI	GGATG	3 cut(s)	10, 211, 230
BseLI	CCNNNNNNNGG	5 cut(s)	240, 339, 411, 690, 974
BseMI	GCAATG	2 cut(s)	1033, 1232
BseMII	CTCAG	2 cut(s)	41, 260
BseNI	ACTGG	5 cut(s)	448, 868, 1021, 1034, 1168
BseRI	GAGGAG	1 cut(s)	309
BseXI	GCAGC	8 cut(s)	456, 832, 841, 850, 900, 1032, 1035, 1294
BseYI	CCCAGC	1 cut(s)	137
BsgI	GTGCAG	1 cut(s)	463
Bsh1236I	CGCG	1 cut(s)	911
BshFI	GGCC	1 cut(s)	944
BshTI	ACCGGT	1 cut(s)	683
BshVI	ATCGAT	1 cut(s)	55
BsiSI	CCGG	5 cut(s)	202, 280, 684, 753, 1253
BslFI	GGGAC	2 cut(s)	972, 1027
BslI	CCNNNNNNNGG	5 cut(s)	240, 339, 411, 690, 974
BsmAI	GTCTC	5 cut(s)	277, 410, 653, 800, 879
BsmFI	GGGAC	2 cut(s)	972, 1027
BsnI	GGCC	1 cut(s)	944
Bso31I	GGTCTC	1 cut(s)	653
Bsp1286I	GDGCHC	1 cut(s)	1259
Bsp1407I	TGTACA	1 cut(s)	504
Bsp143I	GATC	3 cut(s)	661, 1140, 1365
BspACI	CCGC	4 cut(s)	234, 333, 1196, 1220
BspANI	GGCC	1 cut(s)	944
BspCNI	CTCAG	2 cut(s)	40, 261
BspDI	ATCGAT	1 cut(s)	55
BspFNI	CGCG	1 cut(s)	911
BspLI	GGNNCC	2 cut(s)	616, 1367
BspOI	GCTAGC	1 cut(s)	339
BspPI	GGATC	3 cut(s)	669, 1360, 1373
BspTNI	GGTCTC	1 cut(s)	653
BsrDI	GCAATG	2 cut(s)	1033, 1232
BsrFI	RCCGGY	1 cut(s)	683
BsrGI	TGTACA	1 cut(s)	504
BsrI	ACTGG	5 cut(s)	448, 868, 1021, 1034, 1168
BssAI	RCCGGY	1 cut(s)	683
BssECI	CCNNGG	1 cut(s)	618
BssMI	GATC	3 cut(s)	661, 1140, 1365
BssNAI	GTATAC	1 cut(s)	1201
BssT1I	CCWWGG	1 cut(s)	618
Bst1107I	GTATAC	1 cut(s)	1201
Bst2UI	CCWGG	1 cut(s)	559
Bst4CI	ACNGT	6 cut(s)	406, 421, 541, 890, 992, 1269
Bst6I	CTCTTC	1 cut(s)	298
BstAPI	GCANNNNNTGC	1 cut(s)	58
BstAUI	TGTACA	1 cut(s)	504
BstBAI	YACGTR	1 cut(s)	905
BstC8I	GCNNGC	3 cut(s)	63, 293, 337
BstDEI	CTNAG	2 cut(s)	27, 269
BstF5I	GGATG	3 cut(s)	10, 211, 230
BstFNI	CGCG	1 cut(s)	911
BstHHI	GCGC	1 cut(s)	913
BstKTI	GATC	3 cut(s)	664, 1143, 1368
BstMAI	GTCTC	5 cut(s)	277, 410, 653, 800, 879
BstMBI	GATC	3 cut(s)	661, 1140, 1365
BstMWI	GCNNNNNNNGC	9 cut(s)	58, 134, 731, 826, 829, 835, 838, 908, 1316
BstNI	CCWGG	1 cut(s)	559
BstNSI	RCATGY	2 cut(s)	125, 146
BstSCI	CCNGG	3 cut(s)	201, 557, 1252
BstSFI	CTRYAG	1 cut(s)	1210
BstUI	CGCG	1 cut(s)	911
BstV1I	GCAGC	8 cut(s)	456, 832, 841, 850, 900, 1032, 1035, 1294
BstV2I	GAAGAC	2 cut(s)	661, 851
BstX2I	RGATCY	2 cut(s)	661, 1365
BstYI	RGATCY	2 cut(s)	661, 1365
BstZ17I	GTATAC	1 cut(s)	1201
Bsu15I	ATCGAT	1 cut(s)	55
BsuRI	GGCC	1 cut(s)	944
BsuTUI	ATCGAT	1 cut(s)	55
BtgZI	GCGATG	1 cut(s)	912
BtrI	CACGTC	1 cut(s)	798
BtsCI	GGATG	3 cut(s)	10, 211, 230
BtsI	GCAGTG	2 cut(s)	604, 1215
BtsIMutI	CAGTG	2 cut(s)	604, 1215
Cac8I	GCNNGC	3 cut(s)	63, 293, 337
CfoI	GCGC	1 cut(s)	913
Cfr10I	RCCGGY	1 cut(s)	683
Cfr13I	GGNCC	1 cut(s)	1053
ClaI	ATCGAT	1 cut(s)	55
CseI	GACGC	1 cut(s)	29
Csp6I	GTAC	3 cut(s)	407, 505, 1037
CspAI	ACCGGT	1 cut(s)	683
CviAII	CATG	7 cut(s)	122, 143, 220, 517, 530, 1060, 1138
CviQI	GTAC	3 cut(s)	407, 505, 1037
DdeI	CTNAG	2 cut(s)	27, 269
DpnI	GATC	3 cut(s)	663, 1142, 1367
DpnII	GATC	3 cut(s)	661, 1140, 1365
DraI	TTTAAA	1 cut(s)	454
DriI	GACNNNNNGTC	1 cut(s)	1328
EaeI	YGGCCR	1 cut(s)	942
Eam1104I	CTCTTC	1 cut(s)	298
Eam1105I	GACNNNNNGTC	1 cut(s)	1328
EarI	CTCTTC	1 cut(s)	298
EciI	GGCGGA	1 cut(s)	223
Eco130I	CCWWGG	1 cut(s)	618
Eco24I	GRGCYC	1 cut(s)	1259
Eco31I	GGTCTC	1 cut(s)	653
Eco47I	GGWCC	1 cut(s)	1053
EcoRII	CCWGG	1 cut(s)	557
EcoT14I	CCWWGG	1 cut(s)	618
EcoT22I	ATGCAT	1 cut(s)	225
EcoT38I	GRGCYC	1 cut(s)	1259
ErhI	CCWWGG	1 cut(s)	618
FaeI	CATG	7 cut(s)	125, 146, 223, 520, 533, 1063, 1141
FaqI	GGGAC	2 cut(s)	972, 1027
FatI	CATG	7 cut(s)	121, 142, 219, 516, 529, 1059, 1137
FblI	GTMKAC	3 cut(s)	147, 351, 1200
Fnu4HI	GCNGC	9 cut(s)	333, 445, 821, 830, 839, 914, 1021, 1024, 1308
FokI	GGATG	3 cut(s)	17, 217, 218
FriOI	GRGCYC	1 cut(s)	1259
Fsp4HI	GCNGC	9 cut(s)	333, 445, 821, 830, 839, 914, 1021, 1024, 1308
FspBI	CTAG	6 cut(s)	336, 551, 894, 917, 932, 1320
GlaI	GCGC	1 cut(s)	912
GluI	GCNGC	9 cut(s)	333, 445, 821, 830, 839, 914, 1021, 1024, 1308
GsaI	CCCAGC	1 cut(s)	141
GsuI	CTGGAG	2 cut(s)	741, 1151
HaeIII	GGCC	1 cut(s)	944
HapII	CCGG	5 cut(s)	202, 280, 684, 753, 1253
HgaI	GACGC	1 cut(s)	29
HhaI	GCGC	1 cut(s)	913
Hin1II	CATG	7 cut(s)	125, 146, 223, 520, 533, 1063, 1141
Hin6I	GCGC	1 cut(s)	911
HinP1I	GCGC	1 cut(s)	911
HincII	GTYRAC	3 cut(s)	148, 277, 1228
HindII	GTYRAC	3 cut(s)	148, 277, 1228
HinfI	GANTC	5 cut(s)	70, 285, 316, 808, 960
HpaII	CCGG	5 cut(s)	202, 280, 684, 753, 1253
HphI	GGTGA	3 cut(s)	419, 698, 1257
Hpy188I	TCNGA	4 cut(s)	30, 805, 1301, 1358
Hpy188III	TCNNGA	2 cut(s)	383, 511
HpyAV	CCTTC	5 cut(s)	514, 578, 719, 1044, 1379
HpyCH4III	ACNGT	6 cut(s)	406, 421, 541, 890, 992, 1269
HpyCH4IV	ACGT	3 cut(s)	354, 797, 904
HpyF10VI	GCNNNNNNNGC	9 cut(s)	58, 134, 731, 826, 829, 835, 838, 908, 1316
HpyF3I	CTNAG	2 cut(s)	27, 269
HpySE526I	ACGT	3 cut(s)	354, 797, 904
Hsp92II	CATG	7 cut(s)	125, 146, 223, 520, 533, 1063, 1141
HspAI	GCGC	1 cut(s)	911
Kzo9I	GATC	3 cut(s)	661, 1140, 1365
LmnI	GCTCC	5 cut(s)	614, 722, 826, 835, 1174
Lsp1109I	GCAGC	8 cut(s)	456, 832, 841, 850, 900, 1032, 1035, 1294
LweI	GCATC	5 cut(s)	61, 239, 255, 889, 1197
MaeI	CTAG	6 cut(s)	336, 551, 894, 917, 932, 1320
MaeII	ACGT	3 cut(s)	354, 797, 904
MaeIII	GTNAC	2 cut(s)	400, 905
MalI	GATC	3 cut(s)	663, 1142, 1367
MboI	GATC	3 cut(s)	661, 1140, 1365
MboII	GAAGA	7 cut(s)	203, 315, 407, 488, 536, 661, 856
MfeI	CAATTG	1 cut(s)	606
MflI	RGATCY	2 cut(s)	661, 1365
MhlI	GDGCHC	1 cut(s)	1259
MlyI	GAGTC	4 cut(s)	279, 310, 802, 954
MmeI	TCCRAC	3 cut(s)	67, 1152, 1279
MnlI	CCTC	9 cut(s)	162, 264, 330, 339, 694, 978, 1157, 1173, 1362
Mph1103I	ATGCAT	1 cut(s)	225
MseI	TTAA	4 cut(s)	438, 453, 462, 1385
MslI	CAYNNNNRTG	2 cut(s)	753, 1094
MspI	CCGG	5 cut(s)	202, 280, 684, 753, 1253
MspR9I	CCNGG	3 cut(s)	203, 559, 1254
MunI	CAATTG	1 cut(s)	606
MvaI	CCWGG	1 cut(s)	559
MvnI	CGCG	1 cut(s)	911
MwoI	GCNNNNNNNGC	9 cut(s)	58, 134, 731, 826, 829, 835, 838, 908, 1316
NciI	CCSGG	2 cut(s)	203, 1254
NdeII	GATC	3 cut(s)	661, 1140, 1365
NheI	GCTAGC	1 cut(s)	335
NlaIII	CATG	7 cut(s)	125, 146, 223, 520, 533, 1063, 1141
NlaIV	GGNNCC	2 cut(s)	616, 1367
NmuCI	GTSAC	1 cut(s)	400
NsiI	ATGCAT	1 cut(s)	225
NspI	RCATGY	2 cut(s)	125, 146
OliI	CACNNNNGTG	1 cut(s)	753
PciI	ACATGT	1 cut(s)	121
PfeI	GAWTC	1 cut(s)	70
PflFI	GACNNNGTC	1 cut(s)	152
PinAI	ACCGGT	1 cut(s)	683
PkrI	GCNGC	9 cut(s)	334, 446, 822, 831, 840, 915, 1022, 1025, 1309
PleI	GAGTC	4 cut(s)	279, 310, 802, 954
PpsI	GAGTC	4 cut(s)	279, 310, 802, 954
Ppu21I	YACGTR	1 cut(s)	905
PscI	ACATGT	1 cut(s)	121
PshBI	ATTAAT	1 cut(s)	1385
Psp6I	CCWGG	1 cut(s)	557
PspFI	CCCAGC	1 cut(s)	137
PspGI	CCWGG	1 cut(s)	557
PspN4I	GGNNCC	2 cut(s)	616, 1367
PspPI	GGNCC	1 cut(s)	1053
PsrI	GAACNNNNNNTAC	2 cut(s)	488, 520
PsuI	RGATCY	2 cut(s)	661, 1365
PsyI	GACNNNGTC	1 cut(s)	152
RsaI	GTAC	3 cut(s)	408, 506, 1038
RsaNI	GTAC	3 cut(s)	407, 505, 1037
RseI	CAYNNNNRTG	2 cut(s)	753, 1094
SalI	GTCGAC	1 cut(s)	146
SaqAI	TTAA	4 cut(s)	438, 453, 462, 1385
SatI	GCNGC	9 cut(s)	333, 445, 821, 830, 839, 914, 1021, 1024, 1308
Sau3AI	GATC	3 cut(s)	661, 1140, 1365
Sau96I	GGNCC	1 cut(s)	1053
SchI	GAGTC	4 cut(s)	279, 310, 802, 954
ScrFI	CCNGG	3 cut(s)	203, 559, 1254
SduI	GDGCHC	1 cut(s)	1259
SfaNI	GCATC	5 cut(s)	61, 239, 255, 889, 1197
SfcI	CTRYAG	1 cut(s)	1210
SinI	GGWCC	1 cut(s)	1053
SmiMI	CAYNNNNRTG	2 cut(s)	753, 1094
SpeI	ACTAGT	1 cut(s)	931
SsiI	CCGC	4 cut(s)	234, 333, 1196, 1220
SspMI	CTAG	6 cut(s)	336, 551, 894, 917, 932, 1320
StyD4I	CCNGG	3 cut(s)	201, 557, 1252
StyI	CCWWGG	1 cut(s)	618
TaaI	ACNGT	6 cut(s)	406, 421, 541, 890, 992, 1269
TaiI	ACGT	3 cut(s)	357, 800, 907
TaqI	TCGA	5 cut(s)	55, 131, 147, 189, 1109
TatI	WGTACW	2 cut(s)	504, 1036
TauI	GCSGC	1 cut(s)	335
TfiI	GAWTC	1 cut(s)	70
Tru1I	TTAA	4 cut(s)	438, 453, 462, 1385
Tru9I	TTAA	4 cut(s)	438, 453, 462, 1385
TscAI	CASTG	2 cut(s)	604, 1222
TseFI	GTSAC	1 cut(s)	400
TseI	GCWGC	8 cut(s)	444, 820, 829, 838, 913, 1020, 1023, 1307
Tsp45I	GTSAC	1 cut(s)	400
TspDTI	ATGAA	5 cut(s)	533, 536, 693, 1044, 1071
TspGWI	ACGGA	1 cut(s)	315
TspRI	CASTG	2 cut(s)	604, 1222
Tth111I	GACNNNGTC	1 cut(s)	152
VpaK11BI	GGWCC	1 cut(s)	1053
VspI	ATTAAT	1 cut(s)	1385
XapI	RAATTY	3 cut(s)	464, 628, 978
XceI	RCATGY	2 cut(s)	125, 146
XcmI	CCANNNNNNNNNTGG	3 cut(s)	244, 952, 978
XmiI	GTMKAC	3 cut(s)	147, 351, 1200
XspI	CTAG	6 cut(s)	336, 551, 894, 917, 932, 1320
Zsp2I	ATGCAT	1 cut(s)	225

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.