MD07G1241300.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002292
Physical Location & Seq
Forward (+)
31229843 .. 31232694
2852 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1241300.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGGATTCGAGGCTGTCTGAGGCTTCTAAAACAGGAGACATCCAGCTCTTGCACCAGTCGATGGCAGAGAATCCACTTCTGCTCCATAACCTTGCCCTAACTTCCATCGAGAACCCGCTACATGTCGCTTCGCTTGCCGGACATGTCGAATTTGTTCAGGAGATCGTGAAGTTGAGACCAGCATTTGTGCATGAAATGAACCACGATGGGTTCAGCCCCATGCACATCGCGGCAGCCAACGGGTATTTGGAGGTTGTAAGGGAGCTGCTGAGAGTTGACCCGAGACTCTGCCGGATGAAAGGAAGAGACCAATGGACTCCTCTTCATTATGCTGCTGCTAGAGGGAGGGGTGATGTCGTCGGAGAGATGGTTTTGGCTTGCCCGGAAAGTCTCGAAGACGTGACTCTGCATGAGGAGACTTCGTTGCACCTTGCTGTTAATAACTGCCAGTTTGAAGCAGTTAAAATTTTGGTTGAGTTGGTCATGGAACTGAGGAAGGACAACGTTTTGAATATGAAGGACAAATATGGCAATTCAGTTCTTCACGTAGCAACCTGGAAGAAACAACACCAGGTGGTGGAATGGATAGTTGGCAATAGAACAACTCCAAGTGCATTGGAAGTAAACATTGTAAACCAGAGTGGTCTAACACCTCTTGATCTGCTACTAATCTTCACGAGTGAAGCTGGCGATAGGGAAATTGAAGAGATCCTTCGTGGTGCCGGAGCTTCGAGAGCAAAAGACATACACCATACTCCCTCTAATTGTCCCATGGAAACAAATACCCTTCAGCTGCAGCAGCCAAACGACCCGACGGAATACTTCAAGTTCAAAAAGGGGAGAAAATCTGACGAAGATCCAAGCCCGGTGCTGCTAGTTGTCGCTGTACTAGTGGCCACCACCACCTTTCAAGCCGGACTCAGCCCTCCGAACGGCGGTTTGCACTACACCACTGGATCAAACAAGAATGGGACTGGCTGTGGGAGCAATGGTTTAGTAGTTCTCTATATGTTTTTCAACACGATTGGATTTTCGGTTTCGCTTATCATGATCGACTTCCTCACGCGCAACTTCCCTCTGCTGTTTAAGCTTCGATTTTGTATGGTGATGATGTACGGCACGTACACCGTTGCAGTTATCAGCACTGCACCGAAAAGCATGCTGCTCTGCATCACTTCGTCAACTCCATTATTTGTAGTGATTTTACAGTTGTGCTGTTGTATTAATTTGAAGGTGCCTTGGCAAAACCTAATAGGCTCTTTGGCTCCCTTACGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

427

Amino Acids

47.16

Weight (kDa)

6.31

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 8 - 91 4.4e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 40 - 120 2.4e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 40 - 87 2.9e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 69 - 111 1e-05 Ankyrin repeats (many copies)
Ank_2 PF12796 83 - 159 1.6e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 108 - 199 1.2e-09 Ankyrin repeats (3 copies)
PGG PF13962 288 - 380 3e-09 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 719, 1234
AccB7I CCANNNNNTGG 2 cut(s) 61, 577
AccII CGCG 2 cut(s) 230, 1066
AciI CCGC 3 cut(s) 116, 230, 936
AclI AACGTT 1 cut(s) 504
AclWI GGATC 3 cut(s) 703, 851, 964
AcoI YGGCCR 1 cut(s) 894
AcsI RAATTY 2 cut(s) 149, 465
AcuI CTGAAG 1 cut(s) 773
AdeI CACNNNGTG 1 cut(s) 574
AfaI GTAC 3 cut(s) 888, 1115, 1124
AfiI CCNNNNNNNGG 4 cut(s) 61, 577, 932, 935
AflIII ACRYGT 2 cut(s) 121, 142
AgsI TTSAA 8 cut(s) 455, 511, 704, 826, 832, 911, 1018, 1231
AhlI ACTAGT 1 cut(s) 889
AjiI CACGTC 1 cut(s) 400
AjnI CCWGG 2 cut(s) 554, 570
AjuI GAANNNNNNNTTGG 2 cut(s) 573, 605
AluBI AGCT 6 cut(s) 46, 265, 686, 728, 793, 1090
AluI AGCT 6 cut(s) 46, 265, 686, 728, 793, 1090
Alw26I GTCTC 6 cut(s) 30, 169, 277, 300, 395, 410
AlwI GGATC 3 cut(s) 703, 851, 964
Ama87I CYCGRG 1 cut(s) 280
AoxI GGCC 1 cut(s) 894
ApeKI GCWGC 9 cut(s) 233, 265, 332, 335, 793, 796, 799, 871, 1162
ApoI RAATTY 2 cut(s) 149, 465
AseI ATTAAT 1 cut(s) 1224
Asp700I GAANNNNTTC 1 cut(s) 153
AspLEI GCGC 1 cut(s) 1068
AsuC2I CCSGG 2 cut(s) 383, 866
AsuHPI GGTGA 2 cut(s) 362, 1117
AvaI CYCGRG 1 cut(s) 280
BalI TGGCCA 1 cut(s) 896
BanI GGYRCC 2 cut(s) 719, 1234
BauI CACGAG 1 cut(s) 676
BbsI GAAGAC 1 cut(s) 402
BbvI GCAGC 9 cut(s) 245, 252, 319, 322, 780, 808, 811, 858, 1149
BccI CCATC 4 cut(s) 55, 113, 200, 361
BceAI ACGGC 2 cut(s) 949, 1132
BcgI CGANNNNNNTGC 2 cut(s) 1141, 1175
BciT130I CCWGG 2 cut(s) 556, 572
BcnI CCSGG 2 cut(s) 383, 866
BcoDI GTCTC 6 cut(s) 30, 169, 277, 300, 395, 410
BcuI ACTAGT 1 cut(s) 889
BfaI CTAG 3 cut(s) 339, 875, 890
BfmI CTRYAG 1 cut(s) 794
Bme1390I CCNGG 4 cut(s) 383, 556, 572, 866
BmeT110I CYCGRG 1 cut(s) 280
BmgBI CACGTC 1 cut(s) 400
BmiI GGNNCC 3 cut(s) 721, 1236, 1266
BmrFI CCNGG 4 cut(s) 383, 556, 572, 866
BmsI GCATC 1 cut(s) 1179
BpiI GAAGAC 1 cut(s) 402
BpuMI CCSGG 2 cut(s) 383, 866
BsaAI YACGTR 2 cut(s) 547, 1122
BsaI GGTCTC 2 cut(s) 169, 300
BsaJI CCNNGG 2 cut(s) 771, 1238
BsaXI ACNNNNNCTCC 8 cut(s) 66, 96, 354, 384, 407, 437, 976, 1006
Bsc4I CCNNNNNNNGG 4 cut(s) 61, 577, 932, 935
Bse1I ACTGG 4 cut(s) 55, 448, 958, 979
Bse3DI GCAATG 1 cut(s) 994
BseBI CCWGG 2 cut(s) 556, 572
BseDI CCNNGG 2 cut(s) 771, 1238
BseGI GGATG 2 cut(s) 39, 300
BseLI CCNNNNNNNGG 4 cut(s) 61, 577, 932, 935
BseMI GCAATG 1 cut(s) 994
BseMII CTCAG 4 cut(s) 9, 260, 482, 934
BseNI ACTGG 4 cut(s) 55, 448, 958, 979
BseRI GAGGAG 2 cut(s) 309, 428
BseXI GCAGC 9 cut(s) 245, 252, 319, 322, 780, 808, 811, 858, 1149
BsgI GTGCAG 1 cut(s) 1131
Bsh1236I CGCG 2 cut(s) 230, 1066
BshFI GGCC 1 cut(s) 896
BshNI GGYRCC 2 cut(s) 719, 1234
BsiHKCI CYCGRG 1 cut(s) 280
BsiSI CCGG 6 cut(s) 138, 292, 383, 723, 866, 915
BslFI GGGAC 2 cut(s) 753, 985
BslI CCNNNNNNNGG 4 cut(s) 61, 577, 932, 935
BsmAI GTCTC 6 cut(s) 30, 169, 277, 300, 395, 410
BsmFI GGGAC 2 cut(s) 753, 985
BsnI GGCC 1 cut(s) 896
Bso31I GGTCTC 2 cut(s) 169, 300
BsoBI CYCGRG 1 cut(s) 280
Bsp143I GATC 6 cut(s) 162, 658, 708, 856, 956, 1050
Bsp19I CCATGG 1 cut(s) 771
BspACI CCGC 3 cut(s) 116, 230, 936
BspANI GGCC 1 cut(s) 896
BspCNI CTCAG 4 cut(s) 10, 261, 483, 933
BspFNI CGCG 2 cut(s) 230, 1066
BspHI TCATGA 1 cut(s) 1047
BspLI GGNNCC 3 cut(s) 721, 1236, 1266
BspMAI CTGCAG 1 cut(s) 798
BspPI GGATC 3 cut(s) 703, 851, 964
BspT107I GGYRCC 2 cut(s) 719, 1234
BspTNI GGTCTC 2 cut(s) 169, 300
BsrDI GCAATG 1 cut(s) 994
BsrI ACTGG 4 cut(s) 55, 448, 958, 979
BssECI CCNNGG 2 cut(s) 771, 1238
BssMI GATC 6 cut(s) 162, 658, 708, 856, 956, 1050
BssSI CACGAG 1 cut(s) 676
BssT1I CCWWGG 2 cut(s) 771, 1238
Bst2BI CACGAG 1 cut(s) 676
Bst2UI CCWGG 2 cut(s) 556, 572
Bst4CI ACNGT 2 cut(s) 1129, 1209
Bst6I CTCTTC 3 cut(s) 298, 327, 699
BstBAI YACGTR 2 cut(s) 547, 1122
BstC8I GCNNGC 4 cut(s) 135, 379, 688, 1160
BstDEI CTNAG 4 cut(s) 18, 269, 491, 920
BstDSI CCRYGG 1 cut(s) 771
BstF5I GGATG 2 cut(s) 39, 300
BstFNI CGCG 2 cut(s) 230, 1066
BstHHI GCGC 1 cut(s) 1068
BstKTI GATC 6 cut(s) 165, 661, 711, 859, 959, 1053
BstMAI GTCTC 6 cut(s) 30, 169, 277, 300, 395, 410
BstMBI GATC 6 cut(s) 162, 658, 708, 856, 956, 1050
BstMWI GCNNNNNNNGC 5 cut(s) 134, 734, 799, 984, 1087
BstNI CCWGG 2 cut(s) 556, 572
BstNSI RCATGY 3 cut(s) 125, 146, 1162
BstSCI CCNGG 4 cut(s) 381, 554, 570, 864
BstSFI CTRYAG 1 cut(s) 794
BstUI CGCG 2 cut(s) 230, 1066
BstV1I GCAGC 9 cut(s) 245, 252, 319, 322, 780, 808, 811, 858, 1149
BstV2I GAAGAC 1 cut(s) 402
BstX2I RGATCY 2 cut(s) 708, 856
BstYI RGATCY 2 cut(s) 708, 856
BsuRI GGCC 1 cut(s) 896
BtgI CCRYGG 1 cut(s) 771
BtgZI GCGATG 1 cut(s) 211
BtrI CACGTC 1 cut(s) 400
BtsCI GGATG 2 cut(s) 39, 300
BtsI GCAGTG 1 cut(s) 1143
BtsIMutI CAGTG 2 cut(s) 951, 1143
Cac8I GCNNGC 4 cut(s) 135, 379, 688, 1160
CciI TCATGA 1 cut(s) 1047
CfoI GCGC 1 cut(s) 1068
CsiI ACCWGGT 1 cut(s) 570
Csp6I GTAC 3 cut(s) 887, 1114, 1123
CviAII CATG 9 cut(s) 122, 143, 191, 220, 410, 484, 772, 1048, 1159
CviQI GTAC 3 cut(s) 887, 1114, 1123
DdeI CTNAG 4 cut(s) 18, 269, 491, 920
DpnI GATC 6 cut(s) 164, 660, 710, 858, 958, 1052
DpnII GATC 6 cut(s) 162, 658, 708, 856, 956, 1050
DraIII CACNNNGTG 1 cut(s) 574
EaeI YGGCCR 1 cut(s) 894
Eam1104I CTCTTC 3 cut(s) 298, 327, 699
EarI CTCTTC 3 cut(s) 298, 327, 699
Eco130I CCWWGG 2 cut(s) 771, 1238
Eco31I GGTCTC 2 cut(s) 169, 300
Eco57I CTGAAG 1 cut(s) 773
Eco88I CYCGRG 1 cut(s) 280
EcoRII CCWGG 2 cut(s) 554, 570
EcoT14I CCWWGG 2 cut(s) 771, 1238
ErhI CCWWGG 2 cut(s) 771, 1238
FaeI CATG 9 cut(s) 125, 146, 194, 223, 413, 487, 775, 1051, 1162
FalI AAGNNNNNCTT 2 cut(s) 696, 728
FaqI GGGAC 2 cut(s) 753, 985
FatI CATG 9 cut(s) 121, 142, 190, 219, 409, 483, 771, 1047, 1158
FauI CCCGC 1 cut(s) 123
FokI GGATG 2 cut(s) 26, 307
FspBI CTAG 3 cut(s) 339, 875, 890
GlaI GCGC 1 cut(s) 1067
HaeIII GGCC 1 cut(s) 896
HapII CCGG 6 cut(s) 138, 292, 383, 723, 866, 915
HhaI GCGC 1 cut(s) 1068
Hin1II CATG 9 cut(s) 125, 146, 194, 223, 413, 487, 775, 1051, 1162
Hin6I GCGC 1 cut(s) 1066
HinP1I GCGC 1 cut(s) 1066
HincII GTYRAC 2 cut(s) 277, 1182
HindII GTYRAC 2 cut(s) 277, 1182
HindIII AAGCTT 1 cut(s) 1088
HinfI GANTC 6 cut(s) 5, 70, 285, 316, 403, 918
HpaII CCGG 6 cut(s) 138, 292, 383, 723, 866, 915
HphI GGTGA 2 cut(s) 362, 1117
Hpy166II GTNNAC 5 cut(s) 277, 625, 634, 1125, 1182
Hpy188I TCNGA 4 cut(s) 19, 362, 850, 930
Hpy188III TCNNGA 8 cut(s) 109, 158, 166, 392, 656, 676, 732, 1048
Hpy8I GTNNAC 5 cut(s) 277, 625, 634, 1125, 1182
Hpy99I CGWCG 2 cut(s) 362, 817
HpyAV CCTTC 5 cut(s) 490, 511, 722, 797, 1225
HpyCH4III ACNGT 2 cut(s) 1129, 1209
HpyCH4IV ACGT 4 cut(s) 399, 504, 546, 1121
HpyF10VI GCNNNNNNNGC 5 cut(s) 134, 734, 799, 984, 1087
HpyF3I CTNAG 4 cut(s) 18, 269, 491, 920
HpySE526I ACGT 4 cut(s) 399, 504, 546, 1121
Hsp92II CATG 9 cut(s) 125, 146, 194, 223, 413, 487, 775, 1051, 1162
HspAI GCGC 1 cut(s) 1066
Kzo9I GATC 6 cut(s) 162, 658, 708, 856, 956, 1050
LmnI GCTCC 5 cut(s) 87, 262, 725, 984, 1270
Lsp1109I GCAGC 9 cut(s) 245, 252, 319, 322, 780, 808, 811, 858, 1149
LweI GCATC 1 cut(s) 1179
MabI ACCWGGT 1 cut(s) 570
MaeI CTAG 3 cut(s) 339, 875, 890
MaeII ACGT 4 cut(s) 399, 504, 546, 1121
MaeIII GTNAC 1 cut(s) 400
MalI GATC 6 cut(s) 164, 660, 710, 858, 958, 1052
MboI GATC 6 cut(s) 162, 658, 708, 856, 956, 1050
MboII GAAGA 8 cut(s) 314, 315, 407, 533, 571, 664, 716, 866
MflI RGATCY 2 cut(s) 708, 856
MlsI TGGCCA 1 cut(s) 896
MluCI AATT 6 cut(s) 149, 465, 532, 699, 763, 1225
MluNI TGGCCA 1 cut(s) 896
MlyI GAGTC 4 cut(s) 279, 310, 397, 912
MmeI TCCRAC 1 cut(s) 340
Mox20I TGGCCA 1 cut(s) 896
MroXI GAANNNNTTC 1 cut(s) 153
MscI TGGCCA 1 cut(s) 896
MseI TTAA 4 cut(s) 438, 462, 1086, 1224
Msp20I TGGCCA 1 cut(s) 896
MspA1I CMGCKG 1 cut(s) 793
MspI CCGG 6 cut(s) 138, 292, 383, 723, 866, 915
MspR9I CCNGG 4 cut(s) 383, 556, 572, 866
MvaI CCWGG 2 cut(s) 556, 572
MvnI CGCG 2 cut(s) 230, 1066
MwoI GCNNNNNNNGC 5 cut(s) 134, 734, 799, 984, 1087
NciI CCSGG 2 cut(s) 383, 866
NcoI CCATGG 1 cut(s) 771
NdeII GATC 6 cut(s) 162, 658, 708, 856, 956, 1050
NlaIII CATG 9 cut(s) 125, 146, 194, 223, 413, 487, 775, 1051, 1162
NlaIV GGNNCC 3 cut(s) 721, 1236, 1266
NmuCI GTSAC 1 cut(s) 400
NspI RCATGY 3 cut(s) 125, 146, 1162
PaeI GCATGC 1 cut(s) 1162
PagI TCATGA 1 cut(s) 1047
PciI ACATGT 2 cut(s) 121, 142
PdmI GAANNNNTTC 1 cut(s) 153
PfeI GAWTC 2 cut(s) 5, 70
PflMI CCANNNNNTGG 2 cut(s) 61, 577
PleI GAGTC 4 cut(s) 279, 310, 397, 912
PpsI GAGTC 4 cut(s) 279, 310, 397, 912
Ppu21I YACGTR 2 cut(s) 547, 1122
PscI ACATGT 2 cut(s) 121, 142
PshBI ATTAAT 1 cut(s) 1224
Psp1406I AACGTT 1 cut(s) 504
Psp6I CCWGG 2 cut(s) 554, 570
PspGI CCWGG 2 cut(s) 554, 570
PspN4I GGNNCC 3 cut(s) 721, 1236, 1266
PstI CTGCAG 1 cut(s) 798
PsuI RGATCY 2 cut(s) 708, 856
PvuII CAGCTG 1 cut(s) 793
RsaI GTAC 3 cut(s) 888, 1115, 1124
RsaNI GTAC 3 cut(s) 887, 1114, 1123
SaqAI TTAA 4 cut(s) 438, 462, 1086, 1224
Sau3AI GATC 6 cut(s) 162, 658, 708, 856, 956, 1050
SchI GAGTC 4 cut(s) 279, 310, 397, 912
ScrFI CCNGG 4 cut(s) 383, 556, 572, 866
SexAI ACCWGGT 1 cut(s) 570
SfaNI GCATC 1 cut(s) 1179
SfcI CTRYAG 1 cut(s) 794
SpeI ACTAGT 1 cut(s) 889
SphI GCATGC 1 cut(s) 1162
Sse9I AATT 6 cut(s) 149, 465, 532, 699, 763, 1225
SsiI CCGC 3 cut(s) 116, 230, 936
SspMI CTAG 3 cut(s) 339, 875, 890
StyD4I CCNGG 4 cut(s) 381, 554, 570, 864
StyI CCWWGG 2 cut(s) 771, 1238
TaaI ACNGT 2 cut(s) 1129, 1209
TaiI ACGT 4 cut(s) 402, 507, 549, 1124
TaqI TCGA 8 cut(s) 8, 59, 108, 147, 393, 731, 1053, 1093
TasI AATT 6 cut(s) 149, 465, 532, 699, 763, 1225
TatI WGTACW 1 cut(s) 886
TauI GCSGC 1 cut(s) 233
TfiI GAWTC 2 cut(s) 5, 70
Tru1I TTAA 4 cut(s) 438, 462, 1086, 1224
Tru9I TTAA 4 cut(s) 438, 462, 1086, 1224
TscAI CASTG 2 cut(s) 958, 1150
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 9 cut(s) 233, 265, 332, 335, 793, 796, 799, 871, 1162
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 5 cut(s) 207, 212, 311, 314, 530
TspGWI ACGGA 1 cut(s) 830
TspRI CASTG 2 cut(s) 958, 1150
Van91I CCANNNNNTGG 2 cut(s) 61, 577
VspI ATTAAT 1 cut(s) 1224
XapI RAATTY 2 cut(s) 149, 465
XceI RCATGY 3 cut(s) 125, 146, 1162
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XmnI GAANNNNTTC 1 cut(s) 153
XspI CTAG 3 cut(s) 339, 875, 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.