Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003298

Physical Location & Seq

Genomic Coordinates Reverse (-)

2060739 .. 2062825

Gene Structure 2087 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1027100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 498 bp

ATGAAAATGGAGGTGGATTTTTGTGAACAAGAGTTGGCCACGAATGCCGCTGCTTGCAGCCTGTTTCCGGCATCTCTATCTCCGCGGAGACTGCTCAGCAGCAGCGGTCGGAAGAACCATACTTACCAAAAGCTGCCCCAGGAGCCGCTTACGCTCTTCGTCATCAAATTGGATGGTTCTAGCTTTGAGATTCAAGTTTCAAAGACGGCGACCGTGGCCGAACTGAACGAGGCAGTGAAAGATGTTTTCAGTCAGTCGTCGATAGATGAAATGATTTCATGGTCACATGTGTGGGGTAATTTTTGCTTATGCTATGAAGGTCAGAAGCTACTCGATTACAAGGCATTAATCCGACCTTTTGGAATCAAAGATGGCGATAAGATATTTTTTGTTCGACATGCTTCAATAATTAAGTATGAGCGGGAAAAGCGGCGGATAGGGTTGTGGAGGAAAAGGATATGTGACATCTCTACTTATGAACAGCTGGTGAAGTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ubiquitin

Protein Analysis

166

Amino Acids

18.99

Weight (kDa)

8.79

Isoelectric Point (pI)

43.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ubiquitin_4	PF18036	50 - 133	2e-23	Ubiquitin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003298)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g02640 FvH4_6g29570
malus_domestica	MD02G1027100.v1.1 MD15G1168600.v1.1 MD17G1213000.v1.1
prunus_persica	Prupe.3G088400_v2.0.a1 Prupe.3G088400_v2.0.a1 Prupe.3G088400_v2.0.a1 Prupe.7G247900_v2.0.a1 Prupe.7G247900_v2.0.a1 Prupe.7G247900_v2.0.a1 Prupe.7G247900_v2.0.a1
pyrus_communis	pycom02g02210 pycom15g15040 pycom17g21800
rosa_chinensis	RchiOBHm_Chr2g0135131
rosa_laevigata	RLG00000015891 RLG00000019444
rosa_multiflora	Rmu_co8501197.1_g000001 Rmu_sc0001385.1_g000018 Rmu_sc0003543.1_g000006
rosa_roxburghii	Rroxscaffold_2G00109460 Rroxscaffold_2G00153300
rosa_rugosa	Rorug01G0478100 Rorug01G0478200 Rorug02G0323400 Rorug02G0323500.1
rosa_samantha	Rh2AG032200 Rh2AG371600 Rh2BG031300 Rh2BG379900 Rh2CG031700 Rh2CG359000 Rh2DG396500
rosa_wichuraiana	Rw0G013590 Rw2G002510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	421
AccII	CGCG	1 cut(s)	85
AciI	CCGC	8 cut(s)	48, 83, 85, 105, 146, 421, 430, 433
AcoI	YGGCCR	2 cut(s)	36, 216
AfiI	CCNNNNNNNGG	1 cut(s)	67
AflIII	ACRYGT	1 cut(s)	286
AgsI	TTSAA	3 cut(s)	194, 201, 405
AjnI	CCWGG	1 cut(s)	138
AleI	CACNNNNGTG	1 cut(s)	289
AluBI	AGCT	4 cut(s)	133, 183, 328, 484
AluI	AGCT	4 cut(s)	133, 183, 328, 484
Alw26I	GTCTC	1 cut(s)	82
AoxI	GGCC	2 cut(s)	36, 216
ApeKI	GCWGC	5 cut(s)	50, 57, 99, 102, 133
AseI	ATTAAT	1 cut(s)	347
BalI	TGGCCA	1 cut(s)	38
BbvI	GCAGC	5 cut(s)	37, 69, 111, 114, 120
BccI	CCATC	2 cut(s)	167, 365
BceAI	ACGGC	1 cut(s)	222
BciT130I	CCWGG	1 cut(s)	140
BcoDI	GTCTC	1 cut(s)	82
BfaI	CTAG	1 cut(s)	180
BisI	GCNGC	8 cut(s)	48, 51, 58, 100, 103, 134, 146, 431
BlpI	GCTNAGC	1 cut(s)	95
BlsI	GCNGC	8 cut(s)	49, 52, 59, 101, 104, 135, 147, 432
Bme1390I	CCNGG	1 cut(s)	140
BmiI	GGNNCC	1 cut(s)	144
BmrFI	CCNGG	1 cut(s)	140
BmsI	GCATC	1 cut(s)	80
Bpu1102I	GCTNAGC	1 cut(s)	95
BsaJI	CCNNGG	3 cut(s)	83, 138, 213
Bsc4I	CCNNNNNNNGG	1 cut(s)	67
BseBI	CCWGG	1 cut(s)	140
BseDI	CCNNGG	3 cut(s)	83, 138, 213
BseGI	GGATG	1 cut(s)	178
BseLI	CCNNNNNNNGG	1 cut(s)	67
BseMII	CTCAG	1 cut(s)	109
BseXI	GCAGC	5 cut(s)	37, 69, 111, 114, 120
Bsh1236I	CGCG	1 cut(s)	85
Bsh1285I	CGRYCG	2 cut(s)	109, 213
BshFI	GGCC	2 cut(s)	38, 218
BsiEI	CGRYCG	2 cut(s)	109, 213
BsiSI	CCGG	1 cut(s)	68
BslI	CCNNNNNNNGG	1 cut(s)	67
BsmAI	GTCTC	1 cut(s)	82
BsmI	GAATGC	1 cut(s)	49
BsnI	GGCC	2 cut(s)	38, 218
Bsp1720I	GCTNAGC	1 cut(s)	95
BspACI	CCGC	8 cut(s)	48, 83, 85, 105, 146, 421, 430, 433
BspANI	GGCC	2 cut(s)	38, 218
BspCNI	CTCAG	1 cut(s)	108
BspFNI	CGCG	1 cut(s)	85
BspLI	GGNNCC	1 cut(s)	144
BspQI	GCTCTTC	1 cut(s)	161
BsrBI	CCGCTC	1 cut(s)	421
BssECI	CCNNGG	3 cut(s)	83, 138, 213
Bst2UI	CCWGG	1 cut(s)	140
Bst4CI	ACNGT	1 cut(s)	214
Bst6I	CTCTTC	1 cut(s)	161
BstC8I	GCNNGC	1 cut(s)	55
BstDEI	CTNAG	1 cut(s)	95
BstDSI	CCRYGG	2 cut(s)	83, 213
BstF5I	GGATG	1 cut(s)	178
BstFNI	CGCG	1 cut(s)	85
BstMAI	GTCTC	1 cut(s)	82
BstMCI	CGRYCG	2 cut(s)	109, 213
BstMWI	GCNNNNNNNGC	6 cut(s)	44, 91, 142, 151, 215, 427
BstNI	CCWGG	1 cut(s)	140
BstNSI	RCATGY	2 cut(s)	290, 401
BstSCI	CCNGG	1 cut(s)	138
BstUI	CGCG	1 cut(s)	85
BstV1I	GCAGC	5 cut(s)	37, 69, 111, 114, 120
BsuRI	GGCC	2 cut(s)	38, 218
BtgI	CCRYGG	2 cut(s)	83, 213
BtsCI	GGATG	1 cut(s)	178
BtsI	GCAGTG	1 cut(s)	240
BtsIMutI	CAGTG	1 cut(s)	240
Cac8I	GCNNGC	1 cut(s)	55
Cfr42I	CCGCGG	1 cut(s)	86
CviAII	CATG	3 cut(s)	279, 287, 398
CviJI	RGCY	8 cut(s)	38, 60, 133, 145, 183, 218, 328, 484
CviKI_1	RGCY	8 cut(s)	38, 60, 133, 145, 183, 218, 328, 484
DdeI	CTNAG	1 cut(s)	95
EaeI	YGGCCR	2 cut(s)	36, 216
Eam1104I	CTCTTC	1 cut(s)	161
EarI	CTCTTC	1 cut(s)	161
EciI	GGCGGA	1 cut(s)	448
EcoRII	CCWGG	1 cut(s)	138
FaeI	CATG	3 cut(s)	282, 290, 401
FaiI	YATR	9 cut(s)	120, 280, 288, 310, 315, 399, 417, 460, 477
FatI	CATG	3 cut(s)	278, 286, 397
FauI	CCCGC	1 cut(s)	414
Fnu4HI	GCNGC	8 cut(s)	48, 51, 58, 100, 103, 134, 146, 431
FokI	GGATG	1 cut(s)	185
Fsp4HI	GCNGC	8 cut(s)	48, 51, 58, 100, 103, 134, 146, 431
FspBI	CTAG	1 cut(s)	180
GluI	GCNGC	8 cut(s)	48, 51, 58, 100, 103, 134, 146, 431
HaeIII	GGCC	2 cut(s)	38, 218
HapII	CCGG	1 cut(s)	68
Hin1II	CATG	3 cut(s)	282, 290, 401
HinfI	GANTC	2 cut(s)	190, 363
HpaII	CCGG	1 cut(s)	68
Hpy166II	GTNNAC	1 cut(s)	26
Hpy188I	TCNGA	3 cut(s)	111, 324, 353
Hpy8I	GTNNAC	1 cut(s)	26
Hpy99I	CGWCG	1 cut(s)	262
HpyAV	CCTTC	1 cut(s)	311
HpyCH4III	ACNGT	1 cut(s)	214
HpyCH4V	TGCA	1 cut(s)	57
HpyF10VI	GCNNNNNNNGC	6 cut(s)	44, 91, 142, 151, 215, 427
HpyF3I	CTNAG	1 cut(s)	95
Hsp92II	CATG	3 cut(s)	282, 290, 401
KspI	CCGCGG	1 cut(s)	86
LguI	GCTCTTC	1 cut(s)	161
LmnI	GCTCC	1 cut(s)	142
LpnPI	CCDG	5 cut(s)	74, 81, 125, 152, 470
Lsp1109I	GCAGC	5 cut(s)	37, 69, 111, 114, 120
LweI	GCATC	1 cut(s)	80
MaeI	CTAG	1 cut(s)	180
MaeIII	GTNAC	2 cut(s)	282, 461
MbiI	CCGCTC	1 cut(s)	421
MboII	GAAGA	2 cut(s)	124, 148
MlsI	TGGCCA	1 cut(s)	38
MluCI	AATT	3 cut(s)	167, 298, 408
MluNI	TGGCCA	1 cut(s)	38
MmeI	TCCRAC	2 cut(s)	89, 376
MnlI	CCTC	3 cut(s)	4, 223, 441
Mox20I	TGGCCA	1 cut(s)	38
MscI	TGGCCA	1 cut(s)	38
MseI	TTAA	2 cut(s)	347, 411
MslI	CAYNNNNRTG	1 cut(s)	289
Msp20I	TGGCCA	1 cut(s)	38
MspA1I	CMGCKG	4 cut(s)	50, 85, 105, 484
MspI	CCGG	1 cut(s)	68
MspR9I	CCNGG	1 cut(s)	140
Mva1269I	GAATGC	1 cut(s)	49
MvaI	CCWGG	1 cut(s)	140
MvnI	CGCG	1 cut(s)	85
MwoI	GCNNNNNNNGC	6 cut(s)	44, 91, 142, 151, 215, 427
NlaIII	CATG	3 cut(s)	282, 290, 401
NlaIV	GGNNCC	1 cut(s)	144
NmuCI	GTSAC	2 cut(s)	282, 461
NspI	RCATGY	2 cut(s)	290, 401
OliI	CACNNNNGTG	1 cut(s)	289
PciI	ACATGT	1 cut(s)	286
PciSI	GCTCTTC	1 cut(s)	161
PctI	GAATGC	1 cut(s)	49
PfeI	GAWTC	2 cut(s)	190, 363
PkrI	GCNGC	8 cut(s)	49, 52, 59, 101, 104, 135, 147, 432
PscI	ACATGT	1 cut(s)	286
PshBI	ATTAAT	1 cut(s)	347
Psp6I	CCWGG	1 cut(s)	138
PspGI	CCWGG	1 cut(s)	138
PspN4I	GGNNCC	1 cut(s)	144
PsrI	GAACNNNNNNTAC	2 cut(s)	107, 139
PvuII	CAGCTG	1 cut(s)	484
RseI	CAYNNNNRTG	1 cut(s)	289
SacII	CCGCGG	1 cut(s)	86
SapI	GCTCTTC	1 cut(s)	161
SaqAI	TTAA	2 cut(s)	347, 411
SatI	GCNGC	8 cut(s)	48, 51, 58, 100, 103, 134, 146, 431
ScrFI	CCNGG	1 cut(s)	140
SetI	ASST	7 cut(s)	15, 135, 185, 322, 330, 358, 486
SfaNI	GCATC	1 cut(s)	80
Sfr303I	CCGCGG	1 cut(s)	86
SgrBI	CCGCGG	1 cut(s)	86
SmiMI	CAYNNNNRTG	1 cut(s)	289
Sse9I	AATT	3 cut(s)	167, 298, 408
SsiI	CCGC	8 cut(s)	48, 83, 85, 105, 146, 421, 430, 433
SspMI	CTAG	1 cut(s)	180
StyD4I	CCNGG	1 cut(s)	138
TaaI	ACNGT	1 cut(s)	214
TaqI	TCGA	3 cut(s)	260, 333, 394
TasI	AATT	3 cut(s)	167, 298, 408
TauI	GCSGC	3 cut(s)	50, 148, 433
TfiI	GAWTC	2 cut(s)	190, 363
Tru1I	TTAA	2 cut(s)	347, 411
Tru9I	TTAA	2 cut(s)	347, 411
TscAI	CASTG	1 cut(s)	240
TseFI	GTSAC	2 cut(s)	282, 461
TseI	GCWGC	5 cut(s)	50, 57, 99, 102, 133
Tsp45I	GTSAC	2 cut(s)	282, 461
TspDTI	ATGAA	5 cut(s)	17, 267, 282, 330, 492
TspRI	CASTG	1 cut(s)	240
VspI	ATTAAT	1 cut(s)	347
XceI	RCATGY	2 cut(s)	290, 401
XspI	CTAG	1 cut(s)	180

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.