MD02G1028300.v1.1

Plastocyanin-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002685
Physical Location & Seq
Reverse (-)
2171595 .. 2172664
1070 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1028300.v1.1.491

Sequence Viewer

Length: 519 bp
ATGGTGAGTCAGAAGGGATTGATCGGTTGTTTGCTTCTTGTTGTAGTGGCCTTGTGGAAGGGTGCCACAGCTGAAACCTACAAAGTTGGGGATGATTTAGGCTGGACTATTCCTCCAGCTGGCTCTGTTGCCTATTCCACCTGGGCTAACGCAAAGAGATTTCAGATTGGTGATACAATAGAATTCAAATGGAGCGGGGAACACAGAGTAGCTGAAGTAACACAGGCTGATTACGATAGCTGCTCAAAGGATAACCCTCTTGGTTTCTATGATTCCAGCCCTACAAGAATTGTACTGACGTCCAACTTGACCCGTTACTTAATATGCACGGAAGATAGTCACTGCAGTAGATTAGGGCAGAAGGTGACTATCAGAATTGGAGGAGATTATTCTTCATCAGGAGATAATGATGATGATGATCATTGGTGGGAATGGAACGGCGCTTCTTCTCTCAGCATTAATATTGTTGCCGTAATCCTCTGCACTGCACTGGTCATCTCCTTCCTTAGCTTTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

173

Amino Acids

18.88

Weight (kDa)

4.78

Isoelectric Point (pI)

35.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 35 - 116 1.3e-19 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 302
AccB1I GGYRCC 1 cut(s) 62
AccBSI CCGCTC 1 cut(s) 195
AciI CCGC 1 cut(s) 195
AcsI RAATTY 1 cut(s) 182
AcuI CTGAAG 1 cut(s) 234
AcyI GRCGYC 1 cut(s) 299
AfaI GTAC 1 cut(s) 294
AfiI CCNNNNNNNGG 1 cut(s) 119
AgsI TTSAA 1 cut(s) 187
AjnI CCWGG 1 cut(s) 140
AluBI AGCT 5 cut(s) 71, 119, 212, 240, 510
AluI AGCT 5 cut(s) 71, 119, 212, 240, 510
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 1 cut(s) 240
ApoI RAATTY 1 cut(s) 182
AseI ATTAAT 1 cut(s) 459
AspLEI GCGC 1 cut(s) 443
AsuHPI GGTGA 3 cut(s) 16, 182, 376
BanI GGYRCC 1 cut(s) 62
BbvI GCAGC 1 cut(s) 227
BceAI ACGGC 2 cut(s) 454, 455
BciT130I CCWGG 1 cut(s) 142
BclI TGATCA 1 cut(s) 418
BfmI CTRYAG 1 cut(s) 343
BfoI RGCGCY 1 cut(s) 444
BisI GCNGC 1 cut(s) 241
BlsI GCNGC 1 cut(s) 242
Bme1390I CCNGG 1 cut(s) 142
BmiI GGNNCC 1 cut(s) 64
BmrFI CCNGG 1 cut(s) 142
BpmI CTGGAG 1 cut(s) 99
Bpu10I CCTNAGC 1 cut(s) 506
BsaBI GATNNNNATC 1 cut(s) 417
BsaHI GRCGYC 1 cut(s) 299
BsaJI CCNNGG 1 cut(s) 141
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse1I ACTGG 1 cut(s) 495
Bse8I GATNNNNATC 1 cut(s) 417
BseBI CCWGG 1 cut(s) 142
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 1 cut(s) 97
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMII CTCAG 1 cut(s) 466
BseNI ACTGG 1 cut(s) 495
BseRI GAGGAG 1 cut(s) 396
BseXI GCAGC 1 cut(s) 227
BsgI GTGCAG 2 cut(s) 466, 471
BshFI GGCC 1 cut(s) 50
BshNI GGYRCC 1 cut(s) 62
BslI CCNNNNNNNGG 1 cut(s) 119
BsnI GGCC 1 cut(s) 50
Bsp143I GATC 2 cut(s) 21, 418
BspACI CCGC 1 cut(s) 195
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 1 cut(s) 465
BspLI GGNNCC 1 cut(s) 64
BspMAI CTGCAG 1 cut(s) 347
BspT107I GGYRCC 1 cut(s) 62
BsrBI CCGCTC 1 cut(s) 195
BsrI ACTGG 1 cut(s) 495
BssECI CCNNGG 1 cut(s) 141
BssMI GATC 2 cut(s) 21, 418
BssNI GRCGYC 1 cut(s) 299
Bst2UI CCWGG 1 cut(s) 142
BstACI GRCGYC 1 cut(s) 299
BstC8I GCNNGC 1 cut(s) 121
BstDEI CTNAG 2 cut(s) 452, 506
BstF5I GGATG 1 cut(s) 97
BstH2I RGCGCY 1 cut(s) 444
BstHHI GCGC 1 cut(s) 443
BstKTI GATC 2 cut(s) 24, 421
BstMBI GATC 2 cut(s) 21, 418
BstNI CCWGG 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 140
BstSFI CTRYAG 1 cut(s) 343
BstV1I GCAGC 1 cut(s) 227
BsuRI GGCC 1 cut(s) 50
BtsCI GGATG 1 cut(s) 97
BtsI GCAGTG 2 cut(s) 340, 483
BtsIMutI CAGTG 3 cut(s) 340, 483, 488
Cac8I GCNNGC 1 cut(s) 121
CfoI GCGC 1 cut(s) 443
Csp6I GTAC 1 cut(s) 293
CviQI GTAC 1 cut(s) 293
DdeI CTNAG 2 cut(s) 452, 506
DpnI GATC 2 cut(s) 23, 420
DpnII GATC 2 cut(s) 21, 418
Eco57I CTGAAG 1 cut(s) 234
EcoRI GAATTC 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 140
FaiI YATR 2 cut(s) 270, 325
FauI CCCGC 1 cut(s) 188
FbaI TGATCA 1 cut(s) 418
Fnu4HI GCNGC 1 cut(s) 241
FokI GGATG 1 cut(s) 104
Fsp4HI GCNGC 1 cut(s) 241
GlaI GCGC 1 cut(s) 442
GluI GCNGC 1 cut(s) 241
GsuI CTGGAG 1 cut(s) 99
HaeII RGCGCY 1 cut(s) 444
HaeIII GGCC 1 cut(s) 50
HhaI GCGC 1 cut(s) 443
Hin1I GRCGYC 1 cut(s) 299
Hin6I GCGC 1 cut(s) 441
HinP1I GCGC 1 cut(s) 441
HinfI GANTC 2 cut(s) 7, 272
HphI GGTGA 3 cut(s) 16, 182, 376
Hpy188I TCNGA 3 cut(s) 12, 165, 374
Hpy188III TCNNGA 1 cut(s) 399
HpyAV CCTTC 4 cut(s) 7, 52, 355, 511
HpyCH4IV ACGT 1 cut(s) 299
HpyCH4V TGCA 4 cut(s) 327, 345, 483, 488
HpyF3I CTNAG 2 cut(s) 452, 506
HpySE526I ACGT 1 cut(s) 299
Hsp92I GRCGYC 1 cut(s) 299
HspAI GCGC 1 cut(s) 441
Ksp22I TGATCA 1 cut(s) 418
Kzo9I GATC 2 cut(s) 21, 418
LmnI GCTCC 1 cut(s) 192
LpnPI CCDG 9 cut(s) 88, 105, 127, 129, 154, 209, 289, 384, 476
Lsp1109I GCAGC 1 cut(s) 227
MaeII ACGT 1 cut(s) 299
MaeIII GTNAC 4 cut(s) 217, 314, 338, 364
MalI GATC 2 cut(s) 23, 420
MbiI CCGCTC 1 cut(s) 195
MboI GATC 2 cut(s) 21, 418
MboII GAAGA 3 cut(s) 344, 384, 438
MluCI AATT 3 cut(s) 182, 288, 375
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 1 cut(s) 327
MnlI CCTC 4 cut(s) 123, 267, 374, 488
MseI TTAA 3 cut(s) 320, 459, 513
MspA1I CMGCKG 2 cut(s) 71, 119
MspR9I CCNGG 1 cut(s) 142
MvaI CCWGG 1 cut(s) 142
NdeII GATC 2 cut(s) 21, 418
NlaIV GGNNCC 1 cut(s) 64
NmuCI GTSAC 2 cut(s) 338, 364
PfeI GAWTC 1 cut(s) 272
PkrI GCNGC 1 cut(s) 242
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
PshBI ATTAAT 1 cut(s) 459
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 1 cut(s) 64
PstI CTGCAG 1 cut(s) 347
PvuII CAGCTG 2 cut(s) 71, 119
RsaI GTAC 1 cut(s) 294
RsaNI GTAC 1 cut(s) 293
SaqAI TTAA 3 cut(s) 320, 459, 513
SatI GCNGC 1 cut(s) 241
Sau3AI GATC 2 cut(s) 21, 418
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 1 cut(s) 142
SetI ASST 9 cut(s) 73, 80, 121, 143, 214, 242, 302, 366, 512
SfcI CTRYAG 1 cut(s) 343
Sse9I AATT 3 cut(s) 182, 288, 375
SsiI CCGC 1 cut(s) 195
SspI AATATT 1 cut(s) 463
StyD4I CCNGG 1 cut(s) 140
TaiI ACGT 1 cut(s) 302
TasI AATT 3 cut(s) 182, 288, 375
TatI WGTACW 1 cut(s) 292
TfiI GAWTC 1 cut(s) 272
Tru1I TTAA 3 cut(s) 320, 459, 513
Tru9I TTAA 3 cut(s) 320, 459, 513
TscAI CASTG 3 cut(s) 347, 490, 495
TseFI GTSAC 2 cut(s) 338, 364
TseI GCWGC 1 cut(s) 240
Tsp45I GTSAC 2 cut(s) 338, 364
TspDTI ATGAA 1 cut(s) 384
TspGWI ACGGA 1 cut(s) 344
TspRI CASTG 3 cut(s) 347, 490, 495
VspI ATTAAT 1 cut(s) 459
XapI RAATTY 1 cut(s) 182
ZraI GACGTC 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.