RLG00000015915

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002685
Physical Location & Seq
Reverse (-)
2409224 .. 2409835
612 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015915

Sequence Viewer

Length: 492 bp
ATGGCAAGCTGCAGGGATGGTATGATAGCTTGCTTAAGCTTAATGGTTGTTATGGTGGTTTTGATGAAGGGTGCAACAGCAGCAGAATATGTAGTTGGTGATAGCTTGGGCTGGACTCTTCCCCCCAACACCTCCTTCTATTCAGATTGGGCTGCCTCTAAGACATTTCAGTTGGGCGATGTAGTTTCATTCAATTGGACTGGAAATCATACTATGGCAGAAGTAACTAAGGAAGAATATGACAACTGCAGTGACCCGGGAATCTGGATTGCTACTCCGGTGAATGTGAATCTTATTTCTGCTGGTTCTCGCTACTTCATCTGCACCGTTGGTGACCACTGTGAGCAAGGCCAGAAGGTGACCATCACTGTAGGATCCTCCTCTCCAACATCTGCTCCACCACCGCCTTCAGGCGCCCATACTACTGTTGCATCTTTACTACTTGGGCTCATCAGCTCCACACTAGTCATTTCCTTCTTCACTTGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

164

Amino Acids

17.25

Weight (kDa)

4.7

Isoelectric Point (pI)

35.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 38 - 118 9.1e-19 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 413
AciI CCGC 1 cut(s) 404
AclWI GGATC 2 cut(s) 369, 382
AcuI CTGAAG 1 cut(s) 393
AcyI GRCGYC 1 cut(s) 414
AfiI CCNNNNNNNGG 1 cut(s) 410
AflII CTTAAG 1 cut(s) 34
AgsI TTSAA 1 cut(s) 193
AhlI ACTAGT 1 cut(s) 463
AjuI GAANNNNNNNTTGG 4 cut(s) 78, 110, 119, 151
AluBI AGCT 5 cut(s) 9, 29, 39, 105, 456
AluI AGCT 5 cut(s) 9, 29, 39, 105, 456
AlwI GGATC 2 cut(s) 369, 382
Ama87I CYCGRG 1 cut(s) 256
AoxI GGCC 1 cut(s) 349
ApeKI GCWGC 3 cut(s) 9, 80, 152
AspLEI GCGC 1 cut(s) 416
AsuC2I CCSGG 2 cut(s) 257, 258
AsuHPI GGTGA 4 cut(s) 110, 292, 344, 370
AvaI CYCGRG 1 cut(s) 256
BamHI GGATCC 1 cut(s) 374
BanI GGYRCC 1 cut(s) 413
BanII GRGCYC 1 cut(s) 450
BbvI GCAGC 2 cut(s) 92, 139
BccI CCATC 2 cut(s) 11, 371
BcnI CCSGG 2 cut(s) 257, 258
BcuI ACTAGT 1 cut(s) 463
BfaI CTAG 1 cut(s) 464
BfmI CTRYAG 3 cut(s) 10, 247, 369
BfoI RGCGCY 1 cut(s) 417
BfrI CTTAAG 1 cut(s) 34
BisI GCNGC 3 cut(s) 10, 81, 153
BlsI GCNGC 3 cut(s) 11, 82, 154
Bme1390I CCNGG 2 cut(s) 257, 258
BmeT110I CYCGRG 1 cut(s) 256
BmiI GGNNCC 2 cut(s) 376, 415
BmrFI CCNGG 2 cut(s) 257, 258
BmsI GCATC 1 cut(s) 440
BpuMI CCSGG 2 cut(s) 257, 258
BsaHI GRCGYC 1 cut(s) 414
BsaJI CCNNGG 1 cut(s) 256
BsaWI WCCGGW 1 cut(s) 277
BsaXI ACNNNNNCTCC 2 cut(s) 379, 409
Bsc4I CCNNNNNNNGG 1 cut(s) 410
Bse1I ACTGG 1 cut(s) 205
BseDI CCNNGG 1 cut(s) 256
BseGI GGATG 1 cut(s) 22
BseLI CCNNNNNNNGG 1 cut(s) 410
BseNI ACTGG 1 cut(s) 205
BseRI GAGGAG 1 cut(s) 370
BseXI GCAGC 2 cut(s) 92, 139
BsgI GTGCAG 1 cut(s) 307
BshFI GGCC 1 cut(s) 351
BshNI GGYRCC 1 cut(s) 413
BsiHKCI CYCGRG 1 cut(s) 256
BsiSI CCGG 2 cut(s) 257, 278
BslI CCNNNNNNNGG 1 cut(s) 410
BsnI GGCC 1 cut(s) 351
BsoBI CYCGRG 1 cut(s) 256
Bsp1286I GDGCHC 1 cut(s) 450
Bsp143I GATC 1 cut(s) 374
BspACI CCGC 1 cut(s) 404
BspANI GGCC 1 cut(s) 351
BspLI GGNNCC 2 cut(s) 376, 415
BspMAI CTGCAG 2 cut(s) 14, 251
BspPI GGATC 2 cut(s) 369, 382
BspT107I GGYRCC 1 cut(s) 413
BspTI CTTAAG 1 cut(s) 34
BsrI ACTGG 1 cut(s) 205
BssECI CCNNGG 1 cut(s) 256
BssMI GATC 1 cut(s) 374
BssNI GRCGYC 1 cut(s) 414
Bst4CI ACNGT 4 cut(s) 328, 341, 370, 427
Bst6I CTCTTC 1 cut(s) 123
BstACI GRCGYC 1 cut(s) 414
BstAFI CTTAAG 1 cut(s) 34
BstC8I GCNNGC 2 cut(s) 7, 31
BstDEI CTNAG 2 cut(s) 159, 228
BstEII GGTNACC 2 cut(s) 332, 358
BstF5I GGATG 1 cut(s) 22
BstH2I RGCGCY 1 cut(s) 417
BstHHI GCGC 1 cut(s) 416
BstKTI GATC 1 cut(s) 377
BstMBI GATC 1 cut(s) 374
BstMWI GCNNNNNNNGC 1 cut(s) 80
BstNSI RCATGY 1 cut(s) 490
BstPI GGTNACC 2 cut(s) 332, 358
BstSCI CCNGG 2 cut(s) 255, 256
BstSFI CTRYAG 3 cut(s) 10, 247, 369
BstV1I GCAGC 2 cut(s) 92, 139
BstX2I RGATCY 1 cut(s) 374
BstYI RGATCY 1 cut(s) 374
BsuRI GGCC 1 cut(s) 351
BtgZI GCGATG 1 cut(s) 192
BtsCI GGATG 1 cut(s) 22
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 3 cut(s) 256, 337, 366
Cac8I GCNNGC 2 cut(s) 7, 31
CfoI GCGC 1 cut(s) 416
Cfr9I CCCGGG 1 cut(s) 256
CviAII CATG 1 cut(s) 487
CviJI RGCY 9 cut(s) 9, 29, 39, 105, 111, 152, 351, 448, 456
CviKI_1 RGCY 9 cut(s) 9, 29, 39, 105, 111, 152, 351, 448, 456
DdeI CTNAG 2 cut(s) 159, 228
DinI GGCGCC 1 cut(s) 415
DpnI GATC 1 cut(s) 376
DpnII GATC 1 cut(s) 374
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 450
Eco57I CTGAAG 1 cut(s) 393
Eco88I CYCGRG 1 cut(s) 256
Eco91I GGTNACC 2 cut(s) 332, 358
EcoO65I GGTNACC 2 cut(s) 332, 358
EcoT38I GRGCYC 1 cut(s) 450
EgeI GGCGCC 1 cut(s) 415
EheI GGCGCC 1 cut(s) 415
FaeI CATG 1 cut(s) 490
FaiI YATR 8 cut(s) 23, 53, 90, 210, 215, 240, 420, 488
FatI CATG 1 cut(s) 486
Fnu4HI GCNGC 3 cut(s) 10, 81, 153
FokI GGATG 1 cut(s) 29
FriOI GRGCYC 1 cut(s) 450
Fsp4HI GCNGC 3 cut(s) 10, 81, 153
FspBI CTAG 1 cut(s) 464
GlaI GCGC 1 cut(s) 415
GluI GCNGC 3 cut(s) 10, 81, 153
HaeII RGCGCY 1 cut(s) 417
HaeIII GGCC 1 cut(s) 351
HapII CCGG 2 cut(s) 257, 278
HhaI GCGC 1 cut(s) 416
Hin1I GRCGYC 1 cut(s) 414
Hin1II CATG 1 cut(s) 490
Hin6I GCGC 1 cut(s) 414
HinP1I GCGC 1 cut(s) 414
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 3 cut(s) 115, 261, 289
HpaII CCGG 2 cut(s) 257, 278
HphI GGTGA 4 cut(s) 110, 292, 344, 370
Hpy188I TCNGA 1 cut(s) 145
Hpy188III TCNNGA 1 cut(s) 265
HpyAV CCTTC 5 cut(s) 61, 145, 349, 417, 484
HpyCH4III ACNGT 4 cut(s) 328, 341, 370, 427
HpyCH4V TGCA 6 cut(s) 12, 74, 249, 324, 431, 486
HpyF10VI GCNNNNNNNGC 1 cut(s) 80
HpyF3I CTNAG 2 cut(s) 159, 228
Hsp92I GRCGYC 1 cut(s) 414
Hsp92II CATG 1 cut(s) 490
HspAI GCGC 1 cut(s) 414
KasI GGCGCC 1 cut(s) 413
Kzo9I GATC 1 cut(s) 374
LmnI GCTCC 2 cut(s) 400, 461
LpnPI CCDG 8 cut(s) 97, 186, 250, 270, 288, 291, 365, 396
Lsp1109I GCAGC 2 cut(s) 92, 139
LweI GCATC 1 cut(s) 440
MaeI CTAG 1 cut(s) 464
MaeIII GTNAC 4 cut(s) 223, 251, 332, 358
MalI GATC 1 cut(s) 376
MboI GATC 1 cut(s) 374
MboII GAAGA 3 cut(s) 110, 245, 469
MfeI CAATTG 1 cut(s) 193
MflI RGATCY 1 cut(s) 374
MhlI GDGCHC 1 cut(s) 450
MluCI AATT 1 cut(s) 193
Mly113I GGCGCC 1 cut(s) 414
MlyI GAGTC 1 cut(s) 109
MmeI TCCRAC 1 cut(s) 410
MnlI CCTC 4 cut(s) 142, 166, 388, 391
MseI TTAA 2 cut(s) 35, 41
MslI CAYNNNNRTG 1 cut(s) 485
MspCI CTTAAG 1 cut(s) 34
MspI CCGG 2 cut(s) 257, 278
MspR9I CCNGG 2 cut(s) 257, 258
MunI CAATTG 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 80
NarI GGCGCC 1 cut(s) 414
NciI CCSGG 2 cut(s) 257, 258
NdeII GATC 1 cut(s) 374
NlaIII CATG 1 cut(s) 490
NlaIV GGNNCC 2 cut(s) 376, 415
NmuCI GTSAC 3 cut(s) 251, 332, 358
NspI RCATGY 1 cut(s) 490
PfeI GAWTC 2 cut(s) 261, 289
PkrI GCNGC 3 cut(s) 11, 82, 154
PleI GAGTC 1 cut(s) 109
PluTI GGCGCC 1 cut(s) 417
PpsI GAGTC 1 cut(s) 109
PspEI GGTNACC 2 cut(s) 332, 358
PspN4I GGNNCC 2 cut(s) 376, 415
PstI CTGCAG 2 cut(s) 14, 251
PsuI RGATCY 1 cut(s) 374
RseI CAYNNNNRTG 1 cut(s) 485
SaqAI TTAA 2 cut(s) 35, 41
SatI GCNGC 3 cut(s) 10, 81, 153
Sau3AI GATC 1 cut(s) 374
SchI GAGTC 1 cut(s) 109
ScrFI CCNGG 2 cut(s) 257, 258
SduI GDGCHC 1 cut(s) 450
SetI ASST 7 cut(s) 11, 31, 41, 107, 134, 360, 458
SfaNI GCATC 1 cut(s) 440
SfcI CTRYAG 3 cut(s) 10, 247, 369
SfoI GGCGCC 1 cut(s) 415
SmaI CCCGGG 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 485
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
SpeI ACTAGT 1 cut(s) 463
Sse9I AATT 1 cut(s) 193
SsiI CCGC 1 cut(s) 404
SspDI GGCGCC 1 cut(s) 413
SspMI CTAG 1 cut(s) 464
StyD4I CCNGG 2 cut(s) 255, 256
TaaI ACNGT 4 cut(s) 328, 341, 370, 427
TasI AATT 1 cut(s) 193
TfiI GAWTC 2 cut(s) 261, 289
Tru1I TTAA 2 cut(s) 35, 41
Tru9I TTAA 2 cut(s) 35, 41
TscAI CASTG 3 cut(s) 256, 344, 373
TseFI GTSAC 3 cut(s) 251, 332, 358
TseI GCWGC 3 cut(s) 9, 80, 152
Tsp45I GTSAC 3 cut(s) 251, 332, 358
TspDTI ATGAA 3 cut(s) 80, 177, 307
TspMI CCCGGG 1 cut(s) 256
TspRI CASTG 3 cut(s) 256, 344, 373
Vha464I CTTAAG 1 cut(s) 34
XceI RCATGY 1 cut(s) 490
XmaI CCCGGG 1 cut(s) 256
XspI CTAG 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.