Rw2G002730

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002685
Physical Location & Seq
Forward (+)
2413786 .. 2418242
4457 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G002730.1

Sequence Viewer

Length: 450 bp
ATGGATAGCCGTGTGGCTTTGATGAGCTTCGTGATGGTGGCTTTGGTAATGAAGGGTGCAGCTGCTGGAATTCACACAGTCAATTGGACTGTCCCCTCCAATAAGTCAGCTTACACCACTTGGGCTAACACGAGCACTTTCTATCTCAACGACCAACTGCAATTCGGGTGGAGTGGGACGCACAATGTGGCGAATGCAACCAAGGATGAATATGATAACTGCTTAAAGACTGCCAAGGTACTAACAAGTACCTCGCTCACTCTCATCGATCTTAGTACTTCTGGTCCTCTCTACTTCATATGCACCATAGACGATCACTGTGAAAGTGGCCAGAAGCTGGCTATCAACGTTACCAACGTTATCGGTCCCACGTTAACAACTCCACCGCCGCCACCAAGCTCAGCTTCAGCTCTGGCTGTTGGGACTTTATTTGCAATCGAGCACTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

149

Amino Acids

15.89

Weight (kDa)

5.66

Isoelectric Point (pI)

31.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 29 - 111 3.7e-18 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 337
AciI CCGC 2 cut(s) 386, 389
AclI AACGTT 2 cut(s) 348, 357
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 1 cut(s) 69
AcuI CTGAAG 1 cut(s) 390
AdeI CACNNNGTG 1 cut(s) 187
AfaI GTAC 3 cut(s) 240, 250, 277
AfiI CCNNNNNNNGG 1 cut(s) 337
AjuI GAANNNNNNNTTGG 2 cut(s) 388, 420
AluBI AGCT 7 cut(s) 27, 62, 110, 337, 399, 404, 410
AluI AGCT 7 cut(s) 27, 62, 110, 337, 399, 404, 410
Alw21I GWGCWC 2 cut(s) 137, 444
AlwNI CAGNNNCTG 2 cut(s) 65, 337
AoxI GGCC 1 cut(s) 328
ApeKI GCWGC 2 cut(s) 59, 62
ApoI RAATTY 1 cut(s) 69
AspS9I GGNCC 2 cut(s) 284, 365
AvaII GGWCC 2 cut(s) 284, 365
BalI TGGCCA 1 cut(s) 330
BauI CACGAG 1 cut(s) 130
Bbv12I GWGCWC 2 cut(s) 137, 444
BbvI GCAGC 2 cut(s) 49, 71
BccI CCATC 1 cut(s) 28
BisI GCNGC 3 cut(s) 60, 63, 389
BlpI GCTNAGC 1 cut(s) 400
BlsI GCNGC 3 cut(s) 61, 64, 390
BmcAI AGTACT 1 cut(s) 277
Bme18I GGWCC 2 cut(s) 284, 365
BmgT120I GGNCC 2 cut(s) 284, 365
BmiI GGNNCC 1 cut(s) 367
Bpu1102I GCTNAGC 1 cut(s) 400
Bsa29I ATCGAT 1 cut(s) 267
BsaJI CCNNGG 2 cut(s) 201, 234
Bsc4I CCNNNNNNNGG 1 cut(s) 337
BseCI ATCGAT 1 cut(s) 267
BseDI CCNNGG 2 cut(s) 201, 234
BseGI GGATG 1 cut(s) 211
BseLI CCNNNNNNNGG 1 cut(s) 337
BseMII CTCAG 1 cut(s) 414
BseXI GCAGC 2 cut(s) 49, 71
BsgI GTGCAG 1 cut(s) 78
BshFI GGCC 1 cut(s) 330
BshVI ATCGAT 1 cut(s) 267
BsiHKAI GWGCWC 2 cut(s) 137, 444
BslFI GGGAC 4 cut(s) 77, 190, 351, 436
BslI CCNNNNNNNGG 1 cut(s) 337
BsmFI GGGAC 4 cut(s) 77, 190, 351, 436
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 1 cut(s) 330
Bsp1286I GDGCHC 2 cut(s) 137, 444
Bsp143I GATC 2 cut(s) 268, 313
Bsp1720I GCTNAGC 1 cut(s) 400
BspACI CCGC 2 cut(s) 386, 389
BspANI GGCC 1 cut(s) 330
BspCNI CTCAG 1 cut(s) 413
BspDI ATCGAT 1 cut(s) 267
BspLI GGNNCC 1 cut(s) 367
BssECI CCNNGG 2 cut(s) 201, 234
BssMI GATC 2 cut(s) 268, 313
BssSI CACGAG 1 cut(s) 130
BssT1I CCWWGG 2 cut(s) 201, 234
Bst2BI CACGAG 1 cut(s) 130
Bst4CI ACNGT 4 cut(s) 79, 91, 320, 446
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 2 cut(s) 272, 400
BstF5I GGATG 1 cut(s) 211
BstKTI GATC 2 cut(s) 271, 316
BstMBI GATC 2 cut(s) 268, 313
BstV1I GCAGC 2 cut(s) 49, 71
Bsu15I ATCGAT 1 cut(s) 267
BsuRI GGCC 1 cut(s) 330
BsuTUI ATCGAT 1 cut(s) 267
BtsCI GGATG 1 cut(s) 211
BtsIMutI CAGTG 2 cut(s) 316, 442
Cac8I GCNNGC 1 cut(s) 339
CaiI CAGNNNCTG 2 cut(s) 65, 337
Cfr13I GGNCC 2 cut(s) 284, 365
ClaI ATCGAT 1 cut(s) 267
CseI GACGC 1 cut(s) 187
Csp6I GTAC 3 cut(s) 239, 249, 276
CspCI CAANNNNNGTGG 2 cut(s) 149, 184
CviQI GTAC 3 cut(s) 239, 249, 276
DdeI CTNAG 2 cut(s) 272, 400
DpnI GATC 2 cut(s) 270, 315
DpnII GATC 2 cut(s) 268, 313
DraIII CACNNNGTG 1 cut(s) 187
EaeI YGGCCR 1 cut(s) 328
Eco130I CCWWGG 2 cut(s) 201, 234
Eco47I GGWCC 2 cut(s) 284, 365
Eco57I CTGAAG 1 cut(s) 390
EcoRI GAATTC 1 cut(s) 69
EcoT14I CCWWGG 2 cut(s) 201, 234
ErhI CCWWGG 2 cut(s) 201, 234
FaiI YATR 4 cut(s) 213, 299, 301, 308
FalI AAGNNNNNCTT 2 cut(s) 388, 420
FaqI GGGAC 4 cut(s) 77, 190, 351, 436
FauNDI CATATG 1 cut(s) 299
Fnu4HI GCNGC 3 cut(s) 60, 63, 389
FokI GGATG 1 cut(s) 218
Fsp4HI GCNGC 3 cut(s) 60, 63, 389
GluI GCNGC 3 cut(s) 60, 63, 389
HaeIII GGCC 1 cut(s) 330
HgaI GACGC 1 cut(s) 187
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HpaI GTTAAC 1 cut(s) 375
Hpy166II GTNNAC 1 cut(s) 375
Hpy188III TCNNGA 1 cut(s) 31
Hpy8I GTNNAC 1 cut(s) 375
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 4 cut(s) 79, 91, 320, 446
HpyCH4IV ACGT 3 cut(s) 348, 357, 371
HpyCH4V TGCA 5 cut(s) 59, 160, 197, 303, 434
HpyF3I CTNAG 2 cut(s) 272, 400
HpySE526I ACGT 3 cut(s) 348, 357, 371
KspAI GTTAAC 1 cut(s) 375
Kzo9I GATC 2 cut(s) 268, 313
LpnPI CCDG 5 cut(s) 51, 267, 323, 344, 398
Lsp1109I GCAGC 2 cut(s) 49, 71
MaeII ACGT 3 cut(s) 348, 357, 371
MaeIII GTNAC 1 cut(s) 349
MalI GATC 2 cut(s) 270, 315
MboI GATC 2 cut(s) 268, 313
MfeI CAATTG 1 cut(s) 82
MhlI GDGCHC 2 cut(s) 137, 444
MlsI TGGCCA 1 cut(s) 330
MluCI AATT 3 cut(s) 69, 82, 161
MluNI TGGCCA 1 cut(s) 330
MnlI CCTC 3 cut(s) 106, 262, 297
Mox20I TGGCCA 1 cut(s) 330
MscI TGGCCA 1 cut(s) 330
MseI TTAA 3 cut(s) 224, 374, 448
Msp20I TGGCCA 1 cut(s) 330
MspA1I CMGCKG 1 cut(s) 62
MunI CAATTG 1 cut(s) 82
Mva1269I GAATGC 1 cut(s) 199
NdeI CATATG 1 cut(s) 299
NdeII GATC 2 cut(s) 268, 313
NlaIV GGNNCC 1 cut(s) 367
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PctI GAATGC 1 cut(s) 199
PflMI CCANNNNNTGG 1 cut(s) 337
PkrI GCNGC 3 cut(s) 61, 64, 390
Psp1406I AACGTT 2 cut(s) 348, 357
PspN4I GGNNCC 1 cut(s) 367
PspPI GGNCC 2 cut(s) 284, 365
PstNI CAGNNNCTG 2 cut(s) 65, 337
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 3 cut(s) 240, 250, 277
RsaNI GTAC 3 cut(s) 239, 249, 276
SaqAI TTAA 3 cut(s) 224, 374, 448
SatI GCNGC 3 cut(s) 60, 63, 389
Sau3AI GATC 2 cut(s) 268, 313
Sau96I GGNCC 2 cut(s) 284, 365
ScaI AGTACT 1 cut(s) 277
SduI GDGCHC 2 cut(s) 137, 444
SinI GGWCC 2 cut(s) 284, 365
Sse9I AATT 3 cut(s) 69, 82, 161
SsiI CCGC 2 cut(s) 386, 389
StyI CCWWGG 2 cut(s) 201, 234
TaaI ACNGT 4 cut(s) 79, 91, 320, 446
TaiI ACGT 3 cut(s) 351, 360, 374
TaqI TCGA 2 cut(s) 267, 438
TaqII GACCGA 1 cut(s) 353
TasI AATT 3 cut(s) 69, 82, 161
TatI WGTACW 1 cut(s) 275
TauI GCSGC 1 cut(s) 391
Tru1I TTAA 3 cut(s) 224, 374, 448
Tru9I TTAA 3 cut(s) 224, 374, 448
TscAI CASTG 2 cut(s) 323, 449
TseI GCWGC 2 cut(s) 59, 62
TspDTI ATGAA 3 cut(s) 65, 222, 286
TspRI CASTG 2 cut(s) 323, 449
Van91I CCANNNNNTGG 1 cut(s) 337
VpaK11BI GGWCC 2 cut(s) 284, 365
XapI RAATTY 1 cut(s) 69
ZrmI AGTACT 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.