pycom15g15170

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002685
Physical Location & Seq
Reverse (-)
10521171 .. 10521935
765 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g15170.1

Sequence Viewer

Length: 534 bp
ATGGGCGGCCGAAAAGATATGAATATGATGATAGCTTGCTTGGTGGTTATGGCGGTGGCTTCCCCTTCTTTGTTGAATTTGAAGGGTGCAACAGCAGCAGAATACGTAGTTGGTGACAGCTTGGGCTGGACTGTTCCCCCAAACACCTCCTTTTACTCCGACTGGGCCGCCTCAAAGACATTCCAAATAGGAGATACAGTTGTGTTCAACTGGACTGAAAAGTGGGACCATAATGTGGGGGAAGTAGATTCAAAAGAAGAATATGATAACTGCAGTGACCCTGGGGGCGTACTGGGACCATCGTGGAGGATGGAACTCTTTTCTACCGGTTCTCGCTTCTTCATCTGCACCGTTGGTAACCACTGCGAGCAAGGCCAAAAAGTCACCATCACCGTTGGATCACCAACCACCACCTCTTCTCTGTTGGCTCCTCCACCATCATCCTCCCATGCTTCATCTCTCACTACCACCACTCTTGGTTCTTTCGTTGGGCTCATCTCCGTACTAGTAGCAGTAGCTTCCTTCGTCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

178

Amino Acids

18.74

Weight (kDa)

4.84

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 43 - 126 1.2e-17 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 235
AciI CCGC 3 cut(s) 6, 53, 168
AclWI GGATC 1 cut(s) 406
AcoI YGGCCR 1 cut(s) 7
AcsI RAATTY 1 cut(s) 76
AfaI GTAC 2 cut(s) 291, 504
AfiI CCNNNNNNNGG 1 cut(s) 235
AgeI ACCGGT 1 cut(s) 326
AgsI TTSAA 4 cut(s) 76, 82, 208, 252
AhlI ACTAGT 1 cut(s) 505
AjnI CCWGG 1 cut(s) 280
AjuI GAANNNNNNNTTGG 2 cut(s) 93, 125
AluBI AGCT 3 cut(s) 35, 120, 518
AluI AGCT 3 cut(s) 35, 120, 518
AlwI GGATC 1 cut(s) 406
AoxI GGCC 3 cut(s) 7, 165, 373
ApeKI GCWGC 1 cut(s) 95
ApoI RAATTY 1 cut(s) 76
AsiGI ACCGGT 1 cut(s) 326
AspS9I GGNCC 3 cut(s) 165, 226, 296
AsuHPI GGTGA 4 cut(s) 125, 376, 382, 393
AvaII GGWCC 2 cut(s) 226, 296
BaeI ACNNNNGTAYC 2 cut(s) 186, 219
BanII GRGCYC 1 cut(s) 495
BbvI GCAGC 1 cut(s) 107
BccI CCATC 4 cut(s) 304, 307, 395, 445
BciT130I CCWGG 1 cut(s) 282
BcuI ACTAGT 1 cut(s) 505
BfaI CTAG 1 cut(s) 506
BfmI CTRYAG 1 cut(s) 271
BisI GCNGC 3 cut(s) 7, 96, 168
BlsI GCNGC 3 cut(s) 8, 97, 169
Bme1390I CCNGG 1 cut(s) 282
Bme18I GGWCC 2 cut(s) 226, 296
BmgT120I GGNCC 3 cut(s) 165, 226, 296
BmiI GGNNCC 3 cut(s) 227, 297, 429
BmrFI CCNGG 1 cut(s) 282
BmrI ACTGGG 2 cut(s) 172, 302
BmuI ACTGGG 2 cut(s) 172, 302
BsaAI YACGTR 1 cut(s) 106
BsaJI CCNNGG 2 cut(s) 280, 281
BsaWI WCCGGW 1 cut(s) 326
BsaXI ACNNNNNCTCC 2 cut(s) 183, 213
Bsc4I CCNNNNNNNGG 1 cut(s) 235
Bse118I RCCGGY 1 cut(s) 326
Bse1I ACTGG 3 cut(s) 167, 215, 297
BseBI CCWGG 1 cut(s) 282
BseDI CCNNGG 2 cut(s) 280, 281
BseGI GGATG 2 cut(s) 315, 440
BseLI CCNNNNNNNGG 1 cut(s) 235
BseNI ACTGG 3 cut(s) 167, 215, 297
BseRI GAGGAG 1 cut(s) 420
BseX3I CGGCCG 1 cut(s) 7
BseXI GCAGC 1 cut(s) 107
BsgI GTGCAG 1 cut(s) 331
Bsh1285I CGRYCG 1 cut(s) 10
BshFI GGCC 3 cut(s) 9, 167, 375
BshTI ACCGGT 1 cut(s) 326
BsiEI CGRYCG 1 cut(s) 10
BsiSI CCGG 1 cut(s) 327
BslFI GGGAC 2 cut(s) 239, 309
BslI CCNNNNNNNGG 1 cut(s) 235
BsmFI GGGAC 2 cut(s) 239, 309
BsnI GGCC 3 cut(s) 9, 167, 375
Bsp1286I GDGCHC 1 cut(s) 495
Bsp143I GATC 1 cut(s) 398
BspACI CCGC 3 cut(s) 6, 53, 168
BspANI GGCC 3 cut(s) 9, 167, 375
BspLI GGNNCC 3 cut(s) 227, 297, 429
BspMAI CTGCAG 1 cut(s) 275
BspPI GGATC 1 cut(s) 406
BsrFI RCCGGY 1 cut(s) 326
BsrI ACTGG 3 cut(s) 167, 215, 297
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 2 cut(s) 280, 281
BssMI GATC 1 cut(s) 398
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 4 cut(s) 133, 199, 352, 394
Bst6I CTCTTC 1 cut(s) 421
BstBAI YACGTR 1 cut(s) 106
BstC8I GCNNGC 2 cut(s) 37, 368
BstEII GGTNACC 1 cut(s) 356
BstF5I GGATG 2 cut(s) 315, 440
BstKTI GATC 1 cut(s) 401
BstMBI GATC 1 cut(s) 398
BstMCI CGRYCG 1 cut(s) 10
BstMWI GCNNNNNNNGC 2 cut(s) 95, 372
BstNI CCWGG 1 cut(s) 282
BstPI GGTNACC 1 cut(s) 356
BstSCI CCNGG 1 cut(s) 280
BstSFI CTRYAG 1 cut(s) 271
BstSNI TACGTA 1 cut(s) 106
BstV1I GCAGC 1 cut(s) 107
BstZI CGGCCG 1 cut(s) 7
BsuRI GGCC 3 cut(s) 9, 167, 375
BtsCI GGATG 2 cut(s) 315, 440
BtsI GCAGTG 2 cut(s) 280, 361
BtsIMutI CAGTG 2 cut(s) 280, 361
Cac8I GCNNGC 2 cut(s) 37, 368
Cfr10I RCCGGY 1 cut(s) 326
Cfr13I GGNCC 3 cut(s) 165, 226, 296
Csp6I GTAC 2 cut(s) 290, 503
CspAI ACCGGT 1 cut(s) 326
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviAII CATG 1 cut(s) 449
CviQI GTAC 2 cut(s) 290, 503
DpnI GATC 1 cut(s) 400
DpnII GATC 1 cut(s) 398
EaeI YGGCCR 1 cut(s) 7
EagI CGGCCG 1 cut(s) 7
Eam1104I CTCTTC 1 cut(s) 421
EarI CTCTTC 1 cut(s) 421
EclXI CGGCCG 1 cut(s) 7
Eco105I TACGTA 1 cut(s) 106
Eco24I GRGCYC 1 cut(s) 495
Eco47I GGWCC 2 cut(s) 226, 296
Eco52I CGGCCG 1 cut(s) 7
Eco91I GGTNACC 1 cut(s) 356
EcoO65I GGTNACC 1 cut(s) 356
EcoRII CCWGG 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 495
FaeI CATG 1 cut(s) 452
FaiI YATR 6 cut(s) 20, 26, 50, 231, 264, 450
FaqI GGGAC 2 cut(s) 239, 309
FatI CATG 1 cut(s) 448
Fnu4HI GCNGC 3 cut(s) 7, 96, 168
FokI GGATG 2 cut(s) 322, 427
FriOI GRGCYC 1 cut(s) 495
Fsp4HI GCNGC 3 cut(s) 7, 96, 168
FspBI CTAG 1 cut(s) 506
GluI GCNGC 3 cut(s) 7, 96, 168
HaeIII GGCC 3 cut(s) 9, 167, 375
HapII CCGG 1 cut(s) 327
Hin1II CATG 1 cut(s) 452
HinfI GANTC 1 cut(s) 248
HpaII CCGG 1 cut(s) 327
HphI GGTGA 4 cut(s) 125, 376, 382, 393
Hpy188I TCNGA 1 cut(s) 160
Hpy99I CGWCG 1 cut(s) 530
HpyAV CCTTC 3 cut(s) 75, 76, 532
HpyCH4III ACNGT 4 cut(s) 133, 199, 352, 394
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 3 cut(s) 89, 273, 348
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 372
HpySE526I ACGT 1 cut(s) 105
Hsp92II CATG 1 cut(s) 452
Kzo9I GATC 1 cut(s) 398
LmnI GCTCC 1 cut(s) 433
LpnPI CCDG 7 cut(s) 112, 148, 196, 267, 278, 294, 340
Lsp1109I GCAGC 1 cut(s) 107
MaeI CTAG 1 cut(s) 506
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 4 cut(s) 113, 275, 356, 382
MalI GATC 1 cut(s) 400
MboI GATC 1 cut(s) 398
MboII GAAGA 3 cut(s) 269, 331, 408
MhlI GDGCHC 1 cut(s) 495
MluCI AATT 1 cut(s) 76
MmeI TCCRAC 2 cut(s) 183, 376
MnlI CCTC 6 cut(s) 157, 181, 300, 424, 441, 454
MseI TTAA 1 cut(s) 532
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 1 cut(s) 282
MvaI CCWGG 1 cut(s) 282
MwoI GCNNNNNNNGC 2 cut(s) 95, 372
NdeII GATC 1 cut(s) 398
NlaIII CATG 1 cut(s) 452
NlaIV GGNNCC 3 cut(s) 227, 297, 429
NmuCI GTSAC 3 cut(s) 113, 275, 382
PasI CCCWGGG 1 cut(s) 281
PfeI GAWTC 1 cut(s) 248
PflMI CCANNNNNTGG 1 cut(s) 235
PinAI ACCGGT 1 cut(s) 326
PkrI GCNGC 3 cut(s) 8, 97, 169
Ppu21I YACGTR 1 cut(s) 106
Psp6I CCWGG 1 cut(s) 280
PspEI GGTNACC 1 cut(s) 356
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 3 cut(s) 227, 297, 429
PspPI GGNCC 3 cut(s) 165, 226, 296
PstI CTGCAG 1 cut(s) 275
RsaI GTAC 2 cut(s) 291, 504
RsaNI GTAC 2 cut(s) 290, 503
SaqAI TTAA 1 cut(s) 532
SatI GCNGC 3 cut(s) 7, 96, 168
Sau3AI GATC 1 cut(s) 398
Sau96I GGNCC 3 cut(s) 165, 226, 296
ScrFI CCNGG 1 cut(s) 282
SduI GDGCHC 1 cut(s) 495
SetI ASST 6 cut(s) 37, 108, 122, 149, 416, 520
SfcI CTRYAG 1 cut(s) 271
SinI GGWCC 2 cut(s) 226, 296
SnaBI TACGTA 1 cut(s) 106
SpeI ACTAGT 1 cut(s) 505
Sse9I AATT 1 cut(s) 76
SsiI CCGC 3 cut(s) 6, 53, 168
SspMI CTAG 1 cut(s) 506
StyD4I CCNGG 1 cut(s) 280
TaaI ACNGT 4 cut(s) 133, 199, 352, 394
TaiI ACGT 1 cut(s) 108
TasI AATT 1 cut(s) 76
TauI GCSGC 2 cut(s) 9, 170
TfiI GAWTC 1 cut(s) 248
Tru1I TTAA 1 cut(s) 532
Tru9I TTAA 1 cut(s) 532
TscAI CASTG 2 cut(s) 280, 368
TseFI GTSAC 3 cut(s) 113, 275, 382
TseI GCWGC 1 cut(s) 95
Tsp45I GTSAC 3 cut(s) 113, 275, 382
TspDTI ATGAA 3 cut(s) 35, 331, 444
TspGWI ACGGA 1 cut(s) 490
TspRI CASTG 2 cut(s) 280, 368
Van91I CCANNNNNTGG 1 cut(s) 235
VpaK11BI GGWCC 2 cut(s) 226, 296
XapI RAATTY 1 cut(s) 76
XspI CTAG 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.