MD15G1170000.v1.1

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002685
Physical Location & Seq
Reverse (-)
13101827 .. 13102677
851 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1170000.v1.1.491

Sequence Viewer

Length: 534 bp
ATGGGCGGCCGCAAAGATATGAATATGATGATTGCTTGCTTGGTGGTTATGGCGGTGGCTTGCCCTTCTTTGTTGAATTTGAAGGGTGCAACAGCAGTAGAGTACGTAGTAGGTGACAGCTTGGGCTGGACTGTTCCCCCAAACACCTCCTTTTACTCCGACTGGGCCGCCTCAAAGACATTCCAAATAGGAGATACAGTTGTGTTCAACTGGACTGAAAAGTGGGAACATAATGTGGGGGAAGTAGCATCAAAAGAAGAATATGACAACTGCAGTGACCCTGGGATCGTACTGGGACCAGGGGTGAGTACGGAACTCAAATCTACCGGTTCTCGCTTCTTCATCTGCACCGTTGGTAACCACTGCGAGCAAGGCCAAAAAGTCACCATCACCGTTGGATCACCAACCACCACCTCTTCTCTGTTGGCTCCTCCACCATCATCCTCCCATGCTTCATCTCTCACTACCACCACTCTTGGTTCTTTCGTTGGGCTCATCTCCGCACTAGTAGCAGTAGCTTCCTTCGTCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

178

Amino Acids

18.54

Weight (kDa)

5.02

Isoelectric Point (pI)

35.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 43 - 126 7.2e-18 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 6, 10, 53, 168, 501
AclWI GGATC 2 cut(s) 293, 406
AcoI YGGCCR 1 cut(s) 7
AcsI RAATTY 1 cut(s) 76
AfaI GTAC 3 cut(s) 104, 291, 310
AgeI ACCGGT 1 cut(s) 326
AgsI TTSAA 3 cut(s) 76, 82, 208
AhlI ACTAGT 1 cut(s) 505
AjnI CCWGG 2 cut(s) 280, 298
AluBI AGCT 2 cut(s) 120, 518
AluI AGCT 2 cut(s) 120, 518
AlwI GGATC 2 cut(s) 293, 406
AoxI GGCC 3 cut(s) 7, 165, 373
ApoI RAATTY 1 cut(s) 76
AsiGI ACCGGT 1 cut(s) 326
AspS9I GGNCC 2 cut(s) 165, 296
AsuHPI GGTGA 5 cut(s) 125, 316, 376, 382, 393
AvaII GGWCC 1 cut(s) 296
BaeI ACNNNNGTAYC 2 cut(s) 186, 219
BanII GRGCYC 1 cut(s) 495
BccI CCATC 2 cut(s) 395, 445
BciT130I CCWGG 2 cut(s) 282, 300
BcuI ACTAGT 1 cut(s) 505
BfaI CTAG 1 cut(s) 506
BfmI CTRYAG 1 cut(s) 271
BisI GCNGC 3 cut(s) 7, 10, 168
BlsI GCNGC 3 cut(s) 8, 11, 169
Bme1390I CCNGG 2 cut(s) 282, 300
Bme18I GGWCC 1 cut(s) 296
BmgT120I GGNCC 2 cut(s) 165, 296
BmiI GGNNCC 2 cut(s) 297, 429
BmrFI CCNGG 2 cut(s) 282, 300
BmrI ACTGGG 2 cut(s) 172, 302
BmsI GCATC 1 cut(s) 257
BmuI ACTGGG 2 cut(s) 172, 302
BsaAI YACGTR 1 cut(s) 106
BsaJI CCNNGG 3 cut(s) 280, 281, 299
BsaWI WCCGGW 1 cut(s) 326
BsaXI ACNNNNNCTCC 2 cut(s) 183, 213
Bse118I RCCGGY 1 cut(s) 326
Bse1I ACTGG 3 cut(s) 167, 215, 297
BseBI CCWGG 2 cut(s) 282, 300
BseDI CCNNGG 3 cut(s) 280, 281, 299
BseGI GGATG 1 cut(s) 440
BseNI ACTGG 3 cut(s) 167, 215, 297
BseRI GAGGAG 1 cut(s) 420
BseX3I CGGCCG 1 cut(s) 7
BsgI GTGCAG 1 cut(s) 331
Bsh1285I CGRYCG 1 cut(s) 10
BshFI GGCC 3 cut(s) 9, 167, 375
BshTI ACCGGT 1 cut(s) 326
BsiEI CGRYCG 1 cut(s) 10
BsiSI CCGG 1 cut(s) 327
BslFI GGGAC 1 cut(s) 309
BsmFI GGGAC 1 cut(s) 309
BsnI GGCC 3 cut(s) 9, 167, 375
Bsp1286I GDGCHC 1 cut(s) 495
Bsp143I GATC 2 cut(s) 285, 398
BspACI CCGC 5 cut(s) 6, 10, 53, 168, 501
BspANI GGCC 3 cut(s) 9, 167, 375
BspLI GGNNCC 2 cut(s) 297, 429
BspMAI CTGCAG 1 cut(s) 275
BspPI GGATC 2 cut(s) 293, 406
BsrFI RCCGGY 1 cut(s) 326
BsrI ACTGG 3 cut(s) 167, 215, 297
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 3 cut(s) 280, 281, 299
BssMI GATC 2 cut(s) 285, 398
Bst2UI CCWGG 2 cut(s) 282, 300
Bst4CI ACNGT 4 cut(s) 133, 199, 352, 394
Bst6I CTCTTC 1 cut(s) 421
BstBAI YACGTR 1 cut(s) 106
BstC8I GCNNGC 3 cut(s) 37, 61, 368
BstEII GGTNACC 1 cut(s) 356
BstF5I GGATG 1 cut(s) 440
BstKTI GATC 2 cut(s) 288, 401
BstMBI GATC 2 cut(s) 285, 398
BstMCI CGRYCG 1 cut(s) 10
BstMWI GCNNNNNNNGC 2 cut(s) 372, 509
BstNI CCWGG 2 cut(s) 282, 300
BstPI GGTNACC 1 cut(s) 356
BstSCI CCNGG 2 cut(s) 280, 298
BstSFI CTRYAG 1 cut(s) 271
BstSNI TACGTA 1 cut(s) 106
BstZI CGGCCG 1 cut(s) 7
BsuRI GGCC 3 cut(s) 9, 167, 375
BtsCI GGATG 1 cut(s) 440
BtsI GCAGTG 2 cut(s) 280, 361
BtsIMutI CAGTG 2 cut(s) 280, 361
Cac8I GCNNGC 3 cut(s) 37, 61, 368
CciNI GCGGCCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 326
Cfr13I GGNCC 2 cut(s) 165, 296
Csp6I GTAC 3 cut(s) 103, 290, 309
CspAI ACCGGT 1 cut(s) 326
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviAII CATG 1 cut(s) 449
CviJI RGCY 9 cut(s) 9, 59, 120, 126, 167, 375, 428, 493, 518
CviKI_1 RGCY 9 cut(s) 9, 59, 120, 126, 167, 375, 428, 493, 518
CviQI GTAC 3 cut(s) 103, 290, 309
DpnI GATC 2 cut(s) 287, 400
DpnII GATC 2 cut(s) 285, 398
EaeI YGGCCR 1 cut(s) 7
EagI CGGCCG 1 cut(s) 7
Eam1104I CTCTTC 1 cut(s) 421
EarI CTCTTC 1 cut(s) 421
EclXI CGGCCG 1 cut(s) 7
Eco105I TACGTA 1 cut(s) 106
Eco24I GRGCYC 1 cut(s) 495
Eco47I GGWCC 1 cut(s) 296
Eco52I CGGCCG 1 cut(s) 7
Eco91I GGTNACC 1 cut(s) 356
EcoO65I GGTNACC 1 cut(s) 356
EcoRII CCWGG 2 cut(s) 280, 298
EcoT38I GRGCYC 1 cut(s) 495
FaeI CATG 1 cut(s) 452
FaiI YATR 6 cut(s) 20, 26, 50, 231, 264, 450
FaqI GGGAC 1 cut(s) 309
FatI CATG 1 cut(s) 448
Fnu4HI GCNGC 3 cut(s) 7, 10, 168
FokI GGATG 1 cut(s) 427
FriOI GRGCYC 1 cut(s) 495
Fsp4HI GCNGC 3 cut(s) 7, 10, 168
FspBI CTAG 1 cut(s) 506
GluI GCNGC 3 cut(s) 7, 10, 168
HaeIII GGCC 3 cut(s) 9, 167, 375
HapII CCGG 1 cut(s) 327
Hin1II CATG 1 cut(s) 452
HpaII CCGG 1 cut(s) 327
HphI GGTGA 5 cut(s) 125, 316, 376, 382, 393
Hpy188I TCNGA 1 cut(s) 160
Hpy99I CGWCG 1 cut(s) 530
HpyAV CCTTC 3 cut(s) 75, 76, 532
HpyCH4III ACNGT 4 cut(s) 133, 199, 352, 394
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 3 cut(s) 89, 273, 348
HpyF10VI GCNNNNNNNGC 2 cut(s) 372, 509
HpySE526I ACGT 1 cut(s) 105
Hsp92II CATG 1 cut(s) 452
Kzo9I GATC 2 cut(s) 285, 398
LmnI GCTCC 1 cut(s) 433
LpnPI CCDG 9 cut(s) 112, 148, 196, 267, 278, 285, 294, 312, 340
LweI GCATC 1 cut(s) 257
MaeI CTAG 1 cut(s) 506
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 4 cut(s) 113, 275, 356, 382
MalI GATC 2 cut(s) 287, 400
MboI GATC 2 cut(s) 285, 398
MboII GAAGA 3 cut(s) 269, 331, 408
MhlI GDGCHC 1 cut(s) 495
MluCI AATT 1 cut(s) 76
MmeI TCCRAC 2 cut(s) 183, 376
MnlI CCTC 5 cut(s) 157, 181, 424, 441, 454
MseI TTAA 1 cut(s) 532
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 2 cut(s) 282, 300
MvaI CCWGG 2 cut(s) 282, 300
MwoI GCNNNNNNNGC 2 cut(s) 372, 509
NdeII GATC 2 cut(s) 285, 398
NlaIII CATG 1 cut(s) 452
NlaIV GGNNCC 2 cut(s) 297, 429
NmuCI GTSAC 3 cut(s) 113, 275, 382
NotI GCGGCCGC 1 cut(s) 7
PasI CCCWGGG 1 cut(s) 281
PinAI ACCGGT 1 cut(s) 326
PkrI GCNGC 3 cut(s) 8, 11, 169
Ppu21I YACGTR 1 cut(s) 106
Psp6I CCWGG 2 cut(s) 280, 298
PspEI GGTNACC 1 cut(s) 356
PspGI CCWGG 2 cut(s) 280, 298
PspN4I GGNNCC 2 cut(s) 297, 429
PspPI GGNCC 2 cut(s) 165, 296
PstI CTGCAG 1 cut(s) 275
RsaI GTAC 3 cut(s) 104, 291, 310
RsaNI GTAC 3 cut(s) 103, 290, 309
SaqAI TTAA 1 cut(s) 532
SatI GCNGC 3 cut(s) 7, 10, 168
Sau3AI GATC 2 cut(s) 285, 398
Sau96I GGNCC 2 cut(s) 165, 296
ScrFI CCNGG 2 cut(s) 282, 300
SduI GDGCHC 1 cut(s) 495
SetI ASST 6 cut(s) 108, 115, 122, 149, 416, 520
SfaNI GCATC 1 cut(s) 257
SfcI CTRYAG 1 cut(s) 271
SinI GGWCC 1 cut(s) 296
SnaBI TACGTA 1 cut(s) 106
SpeI ACTAGT 1 cut(s) 505
Sse9I AATT 1 cut(s) 76
SsiI CCGC 5 cut(s) 6, 10, 53, 168, 501
SspMI CTAG 1 cut(s) 506
StyD4I CCNGG 2 cut(s) 280, 298
TaaI ACNGT 4 cut(s) 133, 199, 352, 394
TaiI ACGT 1 cut(s) 108
TasI AATT 1 cut(s) 76
TauI GCSGC 3 cut(s) 9, 12, 170
Tru1I TTAA 1 cut(s) 532
Tru9I TTAA 1 cut(s) 532
TscAI CASTG 2 cut(s) 280, 368
TseFI GTSAC 3 cut(s) 113, 275, 382
Tsp45I GTSAC 3 cut(s) 113, 275, 382
TspDTI ATGAA 3 cut(s) 35, 331, 444
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 2 cut(s) 280, 368
VpaK11BI GGWCC 1 cut(s) 296
XapI RAATTY 1 cut(s) 76
XspI CTAG 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.