Rroxscaffold_2G00153090

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002685
Physical Location & Seq
Forward (+)
89898383 .. 89898982
600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00153090.1

Sequence Viewer

Length: 474 bp
ATGGCAAGCTGCAGGGATAGTATGATAGCTTGCTTAAGCTTAATGGTTGTTATGGTGGGTTTGATGAAGCGTGCAACAGCAGCAGAATATGTAGTTGGTGACAGCTTGGGCTGGACTCTTCCCCCAACACCTCGTTCTATTCAGATTGGGCTGCCTCTAAGACATTTCACATTCAATTGGACTGGAAATCATACTATGGCAGAAGCAACTAAGGAAGAATATGACAACTGCAGTGACCCGGGAATCTGGATTGCTACTCCGGTGAATGTGAATCTTATTTCTGCTGGTTCTCGCTACTTCATCTGCACCGTTGGCGACCACTGTGAGCAAGGCCAGAAGGTGACCATCACCGTAGGTTCCTCCTCTCCAACATCTGCTCCACCACCGCCTTCAGGCGCCCATACTACTGTTGCATCTTTACTACTTGGGCTCATCAGCTCCACACTAGTCATTTCCTTCTTCACTTGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

157

Amino Acids

16.73

Weight (kDa)

6.02

Isoelectric Point (pI)

45.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 57 - 112 5.9e-08 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 395
AciI CCGC 1 cut(s) 386
AcuI CTGAAG 1 cut(s) 375
AcyI GRCGYC 1 cut(s) 396
AfiI CCNNNNNNNGG 1 cut(s) 392
AflII CTTAAG 1 cut(s) 34
AgsI TTSAA 1 cut(s) 175
AhlI ACTAGT 1 cut(s) 445
AjuI GAANNNNNNNTTGG 4 cut(s) 78, 110, 118, 150
AluBI AGCT 5 cut(s) 9, 29, 39, 105, 438
AluI AGCT 5 cut(s) 9, 29, 39, 105, 438
Ama87I CYCGRG 1 cut(s) 238
AoxI GGCC 1 cut(s) 331
ApeKI GCWGC 3 cut(s) 9, 80, 151
AspLEI GCGC 1 cut(s) 398
AsuC2I CCSGG 2 cut(s) 239, 240
AsuHPI GGTGA 4 cut(s) 110, 274, 340, 352
AvaI CYCGRG 1 cut(s) 238
BanI GGYRCC 1 cut(s) 395
BanII GRGCYC 1 cut(s) 432
BbvI GCAGC 2 cut(s) 92, 138
BccI CCATC 1 cut(s) 353
BcnI CCSGG 2 cut(s) 239, 240
BcuI ACTAGT 1 cut(s) 445
BfaI CTAG 1 cut(s) 446
BfmI CTRYAG 2 cut(s) 10, 229
BfoI RGCGCY 1 cut(s) 399
BfrI CTTAAG 1 cut(s) 34
BisI GCNGC 3 cut(s) 10, 81, 152
BlsI GCNGC 3 cut(s) 11, 82, 153
Bme1390I CCNGG 2 cut(s) 239, 240
BmeT110I CYCGRG 1 cut(s) 238
BmiI GGNNCC 2 cut(s) 358, 397
BmrFI CCNGG 2 cut(s) 239, 240
BmsI GCATC 1 cut(s) 422
BpuMI CCSGG 2 cut(s) 239, 240
BsaHI GRCGYC 1 cut(s) 396
BsaJI CCNNGG 1 cut(s) 238
BsaWI WCCGGW 1 cut(s) 259
BsaXI ACNNNNNCTCC 2 cut(s) 361, 391
Bsc4I CCNNNNNNNGG 1 cut(s) 392
Bse1I ACTGG 1 cut(s) 187
BseDI CCNNGG 1 cut(s) 238
BseLI CCNNNNNNNGG 1 cut(s) 392
BseNI ACTGG 1 cut(s) 187
BseRI GAGGAG 1 cut(s) 352
BseXI GCAGC 2 cut(s) 92, 138
BsgI GTGCAG 1 cut(s) 289
BshFI GGCC 1 cut(s) 333
BshNI GGYRCC 1 cut(s) 395
BsiHKCI CYCGRG 1 cut(s) 238
BsiSI CCGG 2 cut(s) 239, 260
BslI CCNNNNNNNGG 1 cut(s) 392
BsnI GGCC 1 cut(s) 333
BsoBI CYCGRG 1 cut(s) 238
Bsp1286I GDGCHC 1 cut(s) 432
BspACI CCGC 1 cut(s) 386
BspANI GGCC 1 cut(s) 333
BspLI GGNNCC 2 cut(s) 358, 397
BspMAI CTGCAG 2 cut(s) 14, 233
BspT107I GGYRCC 1 cut(s) 395
BspTI CTTAAG 1 cut(s) 34
BsrI ACTGG 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 238
BssNI GRCGYC 1 cut(s) 396
Bst4CI ACNGT 4 cut(s) 310, 323, 352, 409
Bst6I CTCTTC 1 cut(s) 123
BstACI GRCGYC 1 cut(s) 396
BstAFI CTTAAG 1 cut(s) 34
BstC8I GCNNGC 3 cut(s) 7, 31, 72
BstDEI CTNAG 2 cut(s) 158, 210
BstEII GGTNACC 1 cut(s) 340
BstH2I RGCGCY 1 cut(s) 399
BstHHI GCGC 1 cut(s) 398
BstMWI GCNNNNNNNGC 2 cut(s) 80, 312
BstNSI RCATGY 1 cut(s) 472
BstPI GGTNACC 1 cut(s) 340
BstSCI CCNGG 2 cut(s) 237, 238
BstSFI CTRYAG 2 cut(s) 10, 229
BstV1I GCAGC 2 cut(s) 92, 138
BsuRI GGCC 1 cut(s) 333
BtsI GCAGTG 1 cut(s) 238
BtsIMutI CAGTG 2 cut(s) 238, 319
Cac8I GCNNGC 3 cut(s) 7, 31, 72
CfoI GCGC 1 cut(s) 398
Cfr9I CCCGGG 1 cut(s) 238
CviAII CATG 1 cut(s) 469
CviJI RGCY 9 cut(s) 9, 29, 39, 105, 111, 151, 333, 430, 438
CviKI_1 RGCY 9 cut(s) 9, 29, 39, 105, 111, 151, 333, 430, 438
DdeI CTNAG 2 cut(s) 158, 210
DinI GGCGCC 1 cut(s) 397
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 432
Eco57I CTGAAG 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 238
Eco91I GGTNACC 1 cut(s) 340
EcoO65I GGTNACC 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 432
EgeI GGCGCC 1 cut(s) 397
EheI GGCGCC 1 cut(s) 397
FaeI CATG 1 cut(s) 472
FaiI YATR 8 cut(s) 23, 53, 90, 192, 197, 222, 402, 470
FatI CATG 1 cut(s) 468
Fnu4HI GCNGC 3 cut(s) 10, 81, 152
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 3 cut(s) 10, 81, 152
FspBI CTAG 1 cut(s) 446
GlaI GCGC 1 cut(s) 397
GluI GCNGC 3 cut(s) 10, 81, 152
HaeII RGCGCY 1 cut(s) 399
HaeIII GGCC 1 cut(s) 333
HapII CCGG 2 cut(s) 239, 260
HhaI GCGC 1 cut(s) 398
Hin1I GRCGYC 1 cut(s) 396
Hin1II CATG 1 cut(s) 472
Hin6I GCGC 1 cut(s) 396
HinP1I GCGC 1 cut(s) 396
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 3 cut(s) 115, 243, 271
HpaII CCGG 2 cut(s) 239, 260
HphI GGTGA 4 cut(s) 110, 274, 340, 352
Hpy188I TCNGA 1 cut(s) 144
Hpy188III TCNNGA 1 cut(s) 247
HpyAV CCTTC 3 cut(s) 331, 399, 466
HpyCH4III ACNGT 4 cut(s) 310, 323, 352, 409
HpyCH4V TGCA 6 cut(s) 12, 74, 231, 306, 413, 468
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 312
HpyF3I CTNAG 2 cut(s) 158, 210
Hsp92I GRCGYC 1 cut(s) 396
Hsp92II CATG 1 cut(s) 472
HspAI GCGC 1 cut(s) 396
KasI GGCGCC 1 cut(s) 395
LmnI GCTCC 2 cut(s) 382, 443
LpnPI CCDG 8 cut(s) 97, 168, 232, 252, 270, 273, 347, 378
Lsp1109I GCAGC 2 cut(s) 92, 138
LweI GCATC 1 cut(s) 422
MaeI CTAG 1 cut(s) 446
MaeIII GTNAC 3 cut(s) 98, 233, 340
MboII GAAGA 3 cut(s) 110, 227, 451
MfeI CAATTG 1 cut(s) 175
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 1 cut(s) 175
Mly113I GGCGCC 1 cut(s) 396
MlyI GAGTC 1 cut(s) 109
MmeI TCCRAC 1 cut(s) 392
MnlI CCTC 4 cut(s) 141, 165, 370, 373
MseI TTAA 2 cut(s) 35, 41
MslI CAYNNNNRTG 1 cut(s) 467
MspCI CTTAAG 1 cut(s) 34
MspI CCGG 2 cut(s) 239, 260
MspR9I CCNGG 2 cut(s) 239, 240
MunI CAATTG 1 cut(s) 175
MwoI GCNNNNNNNGC 2 cut(s) 80, 312
NarI GGCGCC 1 cut(s) 396
NciI CCSGG 2 cut(s) 239, 240
NlaIII CATG 1 cut(s) 472
NlaIV GGNNCC 2 cut(s) 358, 397
NmuCI GTSAC 3 cut(s) 98, 233, 340
NspI RCATGY 1 cut(s) 472
PfeI GAWTC 2 cut(s) 243, 271
PkrI GCNGC 3 cut(s) 11, 82, 153
PleI GAGTC 1 cut(s) 109
PluTI GGCGCC 1 cut(s) 399
PpsI GAGTC 1 cut(s) 109
PspEI GGTNACC 1 cut(s) 340
PspN4I GGNNCC 2 cut(s) 358, 397
PstI CTGCAG 2 cut(s) 14, 233
RseI CAYNNNNRTG 1 cut(s) 467
SaqAI TTAA 2 cut(s) 35, 41
SatI GCNGC 3 cut(s) 10, 81, 152
SchI GAGTC 1 cut(s) 109
ScrFI CCNGG 2 cut(s) 239, 240
SduI GDGCHC 1 cut(s) 432
SetI ASST 8 cut(s) 11, 31, 41, 107, 133, 342, 358, 440
SfaNI GCATC 1 cut(s) 422
SfcI CTRYAG 2 cut(s) 10, 229
SfoI GGCGCC 1 cut(s) 397
SmaI CCCGGG 1 cut(s) 240
SmiMI CAYNNNNRTG 1 cut(s) 467
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
SpeI ACTAGT 1 cut(s) 445
Sse9I AATT 1 cut(s) 175
SsiI CCGC 1 cut(s) 386
SspDI GGCGCC 1 cut(s) 395
SspMI CTAG 1 cut(s) 446
StyD4I CCNGG 2 cut(s) 237, 238
TaaI ACNGT 4 cut(s) 310, 323, 352, 409
TasI AATT 1 cut(s) 175
TfiI GAWTC 2 cut(s) 243, 271
Tru1I TTAA 2 cut(s) 35, 41
Tru9I TTAA 2 cut(s) 35, 41
TscAI CASTG 2 cut(s) 238, 326
TseFI GTSAC 3 cut(s) 98, 233, 340
TseI GCWGC 3 cut(s) 9, 80, 151
Tsp45I GTSAC 3 cut(s) 98, 233, 340
TspDTI ATGAA 2 cut(s) 80, 289
TspMI CCCGGG 1 cut(s) 238
TspRI CASTG 2 cut(s) 238, 326
Vha464I CTTAAG 1 cut(s) 34
XceI RCATGY 1 cut(s) 472
XmaI CCCGGG 1 cut(s) 238
XspI CTAG 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.