Rh2AG034900

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002685
Physical Location & Seq
Forward (+)
2557763 .. 2558623
861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG034900.1

Sequence Viewer

Length: 489 bp
ATGGATAGCCGTGTGGCTTTGATGAGCTTCGTGATGGTGGCTTTGGTAATGAAGGGTGCAGCTGCTGGAATTCACACAGTCAATTGGACTGTCCCCTCCAATAAGTCAGCTTACACCACTTGGGCTAACACGAGCACTTTCTATCTCAACGACCAACTGCAATTCGGGTGGAGTGGGACGCACAATGTGGCGAATGCAACCAAGGATGAATATGATAACTGCTTAAAGACTGCCAAGGTACTAACAAGTACCTCGCTCACTCTCATCGATCTTAGTACTTCTGGTCCTCTCTACTTCATATGCACCATAGACGATCACTGTGAAAGTGGCCAGAAGCTGGCTATCAACGTTACCAACGTTATCGGTCCCACGTTAACAACTCCACCGCCGCCACCAAGCTCAGCTTCAGCTCTGGCTGTTGGGACTTTATTTGCAATCGTGAGCTCCATCATAGTCACATCCTTCATTTATCGTTTCATACTATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

162

Amino Acids

17.42

Weight (kDa)

6.23

Isoelectric Point (pI)

27.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 29 - 111 4.7e-18 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 337
AciI CCGC 2 cut(s) 386, 389
AclI AACGTT 2 cut(s) 348, 357
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 1 cut(s) 69
AcuI CTGAAG 1 cut(s) 390
AdeI CACNNNGTG 1 cut(s) 187
AfaI GTAC 3 cut(s) 240, 250, 277
AfiI CCNNNNNNNGG 1 cut(s) 337
AjuI GAANNNNNNNTTGG 2 cut(s) 388, 420
AluBI AGCT 8 cut(s) 27, 62, 110, 337, 399, 404, 410, 444
AluI AGCT 8 cut(s) 27, 62, 110, 337, 399, 404, 410, 444
Alw21I GWGCWC 2 cut(s) 137, 446
AlwNI CAGNNNCTG 2 cut(s) 65, 337
AoxI GGCC 1 cut(s) 328
ApeKI GCWGC 2 cut(s) 59, 62
ApoI RAATTY 1 cut(s) 69
AspS9I GGNCC 2 cut(s) 284, 365
AvaII GGWCC 2 cut(s) 284, 365
BalI TGGCCA 1 cut(s) 330
BanII GRGCYC 1 cut(s) 446
BauI CACGAG 1 cut(s) 130
Bbv12I GWGCWC 2 cut(s) 137, 446
BbvI GCAGC 2 cut(s) 49, 71
BccI CCATC 2 cut(s) 28, 455
BisI GCNGC 3 cut(s) 60, 63, 389
BlpI GCTNAGC 1 cut(s) 400
BlsI GCNGC 3 cut(s) 61, 64, 390
BmcAI AGTACT 1 cut(s) 277
Bme18I GGWCC 2 cut(s) 284, 365
BmgT120I GGNCC 2 cut(s) 284, 365
BmiI GGNNCC 1 cut(s) 367
Bpu1102I GCTNAGC 1 cut(s) 400
Bsa29I ATCGAT 1 cut(s) 267
BsaJI CCNNGG 2 cut(s) 201, 234
Bsc4I CCNNNNNNNGG 1 cut(s) 337
BseCI ATCGAT 1 cut(s) 267
BseDI CCNNGG 2 cut(s) 201, 234
BseGI GGATG 2 cut(s) 211, 458
BseLI CCNNNNNNNGG 1 cut(s) 337
BseMII CTCAG 1 cut(s) 414
BseXI GCAGC 2 cut(s) 49, 71
BsgI GTGCAG 1 cut(s) 78
BshFI GGCC 1 cut(s) 330
BshVI ATCGAT 1 cut(s) 267
BsiHKAI GWGCWC 2 cut(s) 137, 446
BslFI GGGAC 4 cut(s) 77, 190, 351, 436
BslI CCNNNNNNNGG 1 cut(s) 337
BsmFI GGGAC 4 cut(s) 77, 190, 351, 436
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 1 cut(s) 330
Bsp1286I GDGCHC 2 cut(s) 137, 446
Bsp143I GATC 2 cut(s) 268, 313
Bsp1720I GCTNAGC 1 cut(s) 400
BspACI CCGC 2 cut(s) 386, 389
BspANI GGCC 1 cut(s) 330
BspCNI CTCAG 1 cut(s) 413
BspDI ATCGAT 1 cut(s) 267
BspLI GGNNCC 1 cut(s) 367
BssECI CCNNGG 2 cut(s) 201, 234
BssMI GATC 2 cut(s) 268, 313
BssSI CACGAG 1 cut(s) 130
BssT1I CCWWGG 2 cut(s) 201, 234
Bst2BI CACGAG 1 cut(s) 130
Bst4CI ACNGT 3 cut(s) 79, 91, 320
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 2 cut(s) 272, 400
BstF5I GGATG 2 cut(s) 211, 458
BstKTI GATC 2 cut(s) 271, 316
BstMBI GATC 2 cut(s) 268, 313
BstV1I GCAGC 2 cut(s) 49, 71
Bsu15I ATCGAT 1 cut(s) 267
BsuRI GGCC 1 cut(s) 330
BsuTUI ATCGAT 1 cut(s) 267
BtsCI GGATG 2 cut(s) 211, 458
BtsIMutI CAGTG 1 cut(s) 316
Cac8I GCNNGC 1 cut(s) 339
CaiI CAGNNNCTG 2 cut(s) 65, 337
Cfr13I GGNCC 2 cut(s) 284, 365
ClaI ATCGAT 1 cut(s) 267
CseI GACGC 1 cut(s) 187
Csp6I GTAC 3 cut(s) 239, 249, 276
CspCI CAANNNNNGTGG 2 cut(s) 149, 184
CviQI GTAC 3 cut(s) 239, 249, 276
DdeI CTNAG 2 cut(s) 272, 400
DpnI GATC 2 cut(s) 270, 315
DpnII GATC 2 cut(s) 268, 313
DraIII CACNNNGTG 1 cut(s) 187
EaeI YGGCCR 1 cut(s) 328
Ecl136II GAGCTC 1 cut(s) 444
Eco130I CCWWGG 2 cut(s) 201, 234
Eco24I GRGCYC 1 cut(s) 446
Eco47I GGWCC 2 cut(s) 284, 365
Eco53kI GAGCTC 1 cut(s) 444
Eco57I CTGAAG 1 cut(s) 390
EcoICRI GAGCTC 1 cut(s) 444
EcoRI GAATTC 1 cut(s) 69
EcoT14I CCWWGG 2 cut(s) 201, 234
EcoT38I GRGCYC 1 cut(s) 446
ErhI CCWWGG 2 cut(s) 201, 234
FaiI YATR 7 cut(s) 213, 299, 301, 308, 452, 479, 484
FalI AAGNNNNNCTT 2 cut(s) 388, 420
FaqI GGGAC 4 cut(s) 77, 190, 351, 436
FauNDI CATATG 1 cut(s) 299
Fnu4HI GCNGC 3 cut(s) 60, 63, 389
FokI GGATG 2 cut(s) 218, 445
FriOI GRGCYC 1 cut(s) 446
Fsp4HI GCNGC 3 cut(s) 60, 63, 389
GluI GCNGC 3 cut(s) 60, 63, 389
HaeIII GGCC 1 cut(s) 330
HgaI GACGC 1 cut(s) 187
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HpaI GTTAAC 1 cut(s) 375
Hpy166II GTNNAC 1 cut(s) 375
Hpy188III TCNNGA 2 cut(s) 31, 439
Hpy8I GTNNAC 1 cut(s) 375
HpyAV CCTTC 2 cut(s) 46, 472
HpyCH4III ACNGT 3 cut(s) 79, 91, 320
HpyCH4IV ACGT 3 cut(s) 348, 357, 371
HpyCH4V TGCA 5 cut(s) 59, 160, 197, 303, 434
HpyF3I CTNAG 2 cut(s) 272, 400
HpySE526I ACGT 3 cut(s) 348, 357, 371
KspAI GTTAAC 1 cut(s) 375
Kzo9I GATC 2 cut(s) 268, 313
LmnI GCTCC 1 cut(s) 449
LpnPI CCDG 5 cut(s) 51, 267, 323, 344, 398
Lsp1109I GCAGC 2 cut(s) 49, 71
MaeII ACGT 3 cut(s) 348, 357, 371
MaeIII GTNAC 2 cut(s) 349, 454
MalI GATC 2 cut(s) 270, 315
MboI GATC 2 cut(s) 268, 313
MfeI CAATTG 1 cut(s) 82
MhlI GDGCHC 2 cut(s) 137, 446
MlsI TGGCCA 1 cut(s) 330
MluCI AATT 3 cut(s) 69, 82, 161
MluNI TGGCCA 1 cut(s) 330
MnlI CCTC 3 cut(s) 106, 262, 297
Mox20I TGGCCA 1 cut(s) 330
MscI TGGCCA 1 cut(s) 330
MseI TTAA 2 cut(s) 224, 374
Msp20I TGGCCA 1 cut(s) 330
MspA1I CMGCKG 1 cut(s) 62
MunI CAATTG 1 cut(s) 82
Mva1269I GAATGC 1 cut(s) 199
NdeI CATATG 1 cut(s) 299
NdeII GATC 2 cut(s) 268, 313
NlaIV GGNNCC 1 cut(s) 367
NmuCI GTSAC 1 cut(s) 454
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PctI GAATGC 1 cut(s) 199
PflMI CCANNNNNTGG 1 cut(s) 337
PkrI GCNGC 3 cut(s) 61, 64, 390
Psp124BI GAGCTC 1 cut(s) 446
Psp1406I AACGTT 2 cut(s) 348, 357
PspN4I GGNNCC 1 cut(s) 367
PspPI GGNCC 2 cut(s) 284, 365
PstNI CAGNNNCTG 2 cut(s) 65, 337
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 3 cut(s) 240, 250, 277
RsaNI GTAC 3 cut(s) 239, 249, 276
SacI GAGCTC 1 cut(s) 446
SaqAI TTAA 2 cut(s) 224, 374
SatI GCNGC 3 cut(s) 60, 63, 389
Sau3AI GATC 2 cut(s) 268, 313
Sau96I GGNCC 2 cut(s) 284, 365
ScaI AGTACT 1 cut(s) 277
SduI GDGCHC 2 cut(s) 137, 446
SinI GGWCC 2 cut(s) 284, 365
Sse9I AATT 3 cut(s) 69, 82, 161
SsiI CCGC 2 cut(s) 386, 389
SstI GAGCTC 1 cut(s) 446
StyI CCWWGG 2 cut(s) 201, 234
TaaI ACNGT 3 cut(s) 79, 91, 320
TaiI ACGT 3 cut(s) 351, 360, 374
TaqI TCGA 1 cut(s) 267
TaqII GACCGA 1 cut(s) 353
TasI AATT 3 cut(s) 69, 82, 161
TatI WGTACW 1 cut(s) 275
TauI GCSGC 1 cut(s) 391
Tru1I TTAA 2 cut(s) 224, 374
Tru9I TTAA 2 cut(s) 224, 374
TscAI CASTG 1 cut(s) 323
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 2 cut(s) 59, 62
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 5 cut(s) 65, 222, 286, 454, 466
TspRI CASTG 1 cut(s) 323
Van91I CCANNNNNTGG 1 cut(s) 337
VpaK11BI GGWCC 2 cut(s) 284, 365
XapI RAATTY 1 cut(s) 69
ZrmI AGTACT 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.