Rh2CG034800

Cucumber peeling cupredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002685
Physical Location & Seq
Reverse (-)
2681081 .. 2681658
578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG034800.1

Sequence Viewer

Length: 483 bp
ATGGCAAGCTGCAGGGCTAGTATGATAGCTTGCTTAAGCTTAATGGTTGTTATGGTGGGTTTAATAAAGGGTGCAACAGCAGCAGAATATGTAGTTGGTGACAGCTTGGGCTGGACTCTTCCCCCCAACACCTCCTTCTATTCAGATTGGGCTGCCTCTAAGACATTTCAGTTGGGCGATCATTCAATTGGACTGGAAATCATACTATGGGAAGCAACTACGGAAGAATATGACAACTGCAGTGACCCGGGAATCTGGATTACTACTCCGGTGAATGTGAATCTTATTTCTGCTGGTTCTCGCTACTTCATCTGCACCGTTGGCGACCACTGTGAGCAAGGCCAGAAGGTGACCATCACTGTAGGATCATCCTCTCCAACATCTGCTCCACCACCGCCTTCAGGCGCCCATACAACTGTTGCATCTTTACTACTTGGGCTCATCAGCTCCACACTAGTCATTTCCTTCTTCACTTGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

160

Amino Acids

16.87

Weight (kDa)

4.56

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 38 - 115 9.1e-11 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 404
AciI CCGC 1 cut(s) 395
AclWI GGATC 1 cut(s) 373
AcuI CTGAAG 1 cut(s) 384
AcyI GRCGYC 1 cut(s) 405
AfiI CCNNNNNNNGG 1 cut(s) 401
AflII CTTAAG 1 cut(s) 34
AgsI TTSAA 1 cut(s) 186
AhlI ACTAGT 1 cut(s) 454
AjuI GAANNNNNNNTTGG 4 cut(s) 78, 110, 119, 151
AluBI AGCT 5 cut(s) 9, 29, 39, 105, 447
AluI AGCT 5 cut(s) 9, 29, 39, 105, 447
AlwI GGATC 1 cut(s) 373
Ama87I CYCGRG 1 cut(s) 247
AoxI GGCC 1 cut(s) 340
ApeKI GCWGC 3 cut(s) 9, 80, 152
AspLEI GCGC 1 cut(s) 407
AsuC2I CCSGG 2 cut(s) 248, 249
AsuHPI GGTGA 3 cut(s) 110, 283, 361
AvaI CYCGRG 1 cut(s) 247
BanI GGYRCC 1 cut(s) 404
BanII GRGCYC 1 cut(s) 441
BbvI GCAGC 2 cut(s) 92, 139
BccI CCATC 1 cut(s) 362
BcnI CCSGG 2 cut(s) 248, 249
BcuI ACTAGT 1 cut(s) 454
BfaI CTAG 2 cut(s) 18, 455
BfmI CTRYAG 3 cut(s) 10, 238, 360
BfoI RGCGCY 1 cut(s) 408
BfrI CTTAAG 1 cut(s) 34
BisI GCNGC 3 cut(s) 10, 81, 153
BlsI GCNGC 3 cut(s) 11, 82, 154
Bme1390I CCNGG 2 cut(s) 248, 249
BmeT110I CYCGRG 1 cut(s) 247
BmiI GGNNCC 1 cut(s) 406
BmrFI CCNGG 2 cut(s) 248, 249
BmsI GCATC 1 cut(s) 431
BpuMI CCSGG 2 cut(s) 248, 249
BsaHI GRCGYC 1 cut(s) 405
BsaJI CCNNGG 1 cut(s) 247
BsaWI WCCGGW 1 cut(s) 268
BsaXI ACNNNNNCTCC 2 cut(s) 370, 400
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse1I ACTGG 1 cut(s) 198
BseDI CCNNGG 1 cut(s) 247
BseGI GGATG 1 cut(s) 368
BseLI CCNNNNNNNGG 1 cut(s) 401
BseNI ACTGG 1 cut(s) 198
BseXI GCAGC 2 cut(s) 92, 139
BsgI GTGCAG 1 cut(s) 298
BshFI GGCC 1 cut(s) 342
BshNI GGYRCC 1 cut(s) 404
BsiHKCI CYCGRG 1 cut(s) 247
BsiSI CCGG 2 cut(s) 248, 269
BslI CCNNNNNNNGG 1 cut(s) 401
BsnI GGCC 1 cut(s) 342
BsoBI CYCGRG 1 cut(s) 247
Bsp1286I GDGCHC 1 cut(s) 441
Bsp143I GATC 2 cut(s) 178, 365
BspACI CCGC 1 cut(s) 395
BspANI GGCC 1 cut(s) 342
BspLI GGNNCC 1 cut(s) 406
BspMAI CTGCAG 2 cut(s) 14, 242
BspPI GGATC 1 cut(s) 373
BspT107I GGYRCC 1 cut(s) 404
BspTI CTTAAG 1 cut(s) 34
BsrI ACTGG 1 cut(s) 198
BssECI CCNNGG 1 cut(s) 247
BssMI GATC 2 cut(s) 178, 365
BssNI GRCGYC 1 cut(s) 405
Bst4CI ACNGT 4 cut(s) 319, 332, 361, 418
Bst6I CTCTTC 1 cut(s) 123
BstACI GRCGYC 1 cut(s) 405
BstAFI CTTAAG 1 cut(s) 34
BstC8I GCNNGC 2 cut(s) 7, 31
BstDEI CTNAG 1 cut(s) 159
BstEII GGTNACC 1 cut(s) 349
BstF5I GGATG 1 cut(s) 368
BstH2I RGCGCY 1 cut(s) 408
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 2 cut(s) 181, 368
BstMBI GATC 2 cut(s) 178, 365
BstMWI GCNNNNNNNGC 2 cut(s) 80, 321
BstNSI RCATGY 1 cut(s) 481
BstPI GGTNACC 1 cut(s) 349
BstSCI CCNGG 2 cut(s) 246, 247
BstSFI CTRYAG 3 cut(s) 10, 238, 360
BstV1I GCAGC 2 cut(s) 92, 139
BsuRI GGCC 1 cut(s) 342
BtsCI GGATG 1 cut(s) 368
BtsI GCAGTG 1 cut(s) 247
BtsIMutI CAGTG 3 cut(s) 247, 328, 357
Cac8I GCNNGC 2 cut(s) 7, 31
CfoI GCGC 1 cut(s) 407
Cfr9I CCCGGG 1 cut(s) 247
CviAII CATG 1 cut(s) 478
DdeI CTNAG 1 cut(s) 159
DinI GGCGCC 1 cut(s) 406
DpnI GATC 2 cut(s) 180, 367
DpnII GATC 2 cut(s) 178, 365
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 441
Eco57I CTGAAG 1 cut(s) 384
Eco88I CYCGRG 1 cut(s) 247
Eco91I GGTNACC 1 cut(s) 349
EcoO65I GGTNACC 1 cut(s) 349
EcoT38I GRGCYC 1 cut(s) 441
EgeI GGCGCC 1 cut(s) 406
EheI GGCGCC 1 cut(s) 406
FaeI CATG 1 cut(s) 481
FaiI YATR 8 cut(s) 23, 53, 90, 203, 208, 231, 411, 479
FatI CATG 1 cut(s) 477
Fnu4HI GCNGC 3 cut(s) 10, 81, 153
FokI GGATG 1 cut(s) 355
FriOI GRGCYC 1 cut(s) 441
Fsp4HI GCNGC 3 cut(s) 10, 81, 153
FspBI CTAG 2 cut(s) 18, 455
GlaI GCGC 1 cut(s) 406
GluI GCNGC 3 cut(s) 10, 81, 153
HaeII RGCGCY 1 cut(s) 408
HaeIII GGCC 1 cut(s) 342
HapII CCGG 2 cut(s) 248, 269
HhaI GCGC 1 cut(s) 407
Hin1I GRCGYC 1 cut(s) 405
Hin1II CATG 1 cut(s) 481
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 3 cut(s) 115, 252, 280
HpaII CCGG 2 cut(s) 248, 269
HphI GGTGA 3 cut(s) 110, 283, 361
Hpy188I TCNGA 1 cut(s) 145
Hpy188III TCNNGA 1 cut(s) 256
HpyAV CCTTC 4 cut(s) 145, 340, 408, 475
HpyCH4III ACNGT 4 cut(s) 319, 332, 361, 418
HpyCH4V TGCA 6 cut(s) 12, 74, 240, 315, 422, 477
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 321
HpyF3I CTNAG 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 405
Hsp92II CATG 1 cut(s) 481
HspAI GCGC 1 cut(s) 405
KasI GGCGCC 1 cut(s) 404
Kzo9I GATC 2 cut(s) 178, 365
LmnI GCTCC 2 cut(s) 391, 452
LpnPI CCDG 8 cut(s) 97, 179, 241, 261, 279, 282, 356, 387
Lsp1109I GCAGC 2 cut(s) 92, 139
LweI GCATC 1 cut(s) 431
MaeI CTAG 2 cut(s) 18, 455
MaeIII GTNAC 3 cut(s) 98, 242, 349
MalI GATC 2 cut(s) 180, 367
MboI GATC 2 cut(s) 178, 365
MboII GAAGA 3 cut(s) 110, 236, 460
MfeI CAATTG 1 cut(s) 186
MhlI GDGCHC 1 cut(s) 441
MluCI AATT 1 cut(s) 186
Mly113I GGCGCC 1 cut(s) 405
MlyI GAGTC 1 cut(s) 109
MmeI TCCRAC 1 cut(s) 401
MnlI CCTC 3 cut(s) 142, 166, 382
MseI TTAA 3 cut(s) 35, 41, 62
MslI CAYNNNNRTG 1 cut(s) 476
MspCI CTTAAG 1 cut(s) 34
MspI CCGG 2 cut(s) 248, 269
MspR9I CCNGG 2 cut(s) 248, 249
MunI CAATTG 1 cut(s) 186
MwoI GCNNNNNNNGC 2 cut(s) 80, 321
NarI GGCGCC 1 cut(s) 405
NciI CCSGG 2 cut(s) 248, 249
NdeII GATC 2 cut(s) 178, 365
NlaIII CATG 1 cut(s) 481
NlaIV GGNNCC 1 cut(s) 406
NmuCI GTSAC 3 cut(s) 98, 242, 349
NspI RCATGY 1 cut(s) 481
PfeI GAWTC 2 cut(s) 252, 280
PkrI GCNGC 3 cut(s) 11, 82, 154
PleI GAGTC 1 cut(s) 109
PluTI GGCGCC 1 cut(s) 408
PpsI GAGTC 1 cut(s) 109
PspEI GGTNACC 1 cut(s) 349
PspN4I GGNNCC 1 cut(s) 406
PstI CTGCAG 2 cut(s) 14, 242
RseI CAYNNNNRTG 1 cut(s) 476
SaqAI TTAA 3 cut(s) 35, 41, 62
SatI GCNGC 3 cut(s) 10, 81, 153
Sau3AI GATC 2 cut(s) 178, 365
SchI GAGTC 1 cut(s) 109
ScrFI CCNGG 2 cut(s) 248, 249
SduI GDGCHC 1 cut(s) 441
SetI ASST 7 cut(s) 11, 31, 41, 107, 134, 351, 449
SfaNI GCATC 1 cut(s) 431
SfcI CTRYAG 3 cut(s) 10, 238, 360
SfoI GGCGCC 1 cut(s) 406
SmaI CCCGGG 1 cut(s) 249
SmiMI CAYNNNNRTG 1 cut(s) 476
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
SpeI ACTAGT 1 cut(s) 454
Sse9I AATT 1 cut(s) 186
SsiI CCGC 1 cut(s) 395
SspDI GGCGCC 1 cut(s) 404
SspMI CTAG 2 cut(s) 18, 455
StyD4I CCNGG 2 cut(s) 246, 247
TaaI ACNGT 4 cut(s) 319, 332, 361, 418
TasI AATT 1 cut(s) 186
TfiI GAWTC 2 cut(s) 252, 280
Tru1I TTAA 3 cut(s) 35, 41, 62
Tru9I TTAA 3 cut(s) 35, 41, 62
TscAI CASTG 3 cut(s) 247, 335, 364
TseFI GTSAC 3 cut(s) 98, 242, 349
TseI GCWGC 3 cut(s) 9, 80, 152
Tsp45I GTSAC 3 cut(s) 98, 242, 349
TspDTI ATGAA 1 cut(s) 298
TspGWI ACGGA 1 cut(s) 236
TspMI CCCGGG 1 cut(s) 247
TspRI CASTG 3 cut(s) 247, 335, 364
Vha464I CTTAAG 1 cut(s) 34
XceI RCATGY 1 cut(s) 481
XmaI CCCGGG 1 cut(s) 247
XspI CTAG 2 cut(s) 18, 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.