MD02G1130200.v1.1

Thaumatin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003319
Physical Location & Seq
Reverse (-)
10489816 .. 10492139
2324 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1130200.v1.1.491

Sequence Viewer

Length: 912 bp
ATGGCTACTCCAATTCCTTACCACTTCACCTTCATTCTTCTAATTATTACCGAAGGGGTGTTAGGGGCCACATTCACGTTCACAAATAAGTGCGACTTCACAGTATGGCCGGGAATTCTAGCCAGCGCCGGCAGCGACAAGCTCGACAGCACCGGCTTCGAGCTTCCCAAAGGCAGTTCCCGCGTTTTCCAGGCCCCGACCGGCTGGTCCGGTCGCTTCTGGGGCCGAACCGGCTGTACCTTTGACGGTTCGGGTCGGGGTTCTTGTTCGACCGGGGACTGCGGCTCGGGCGAGCTCGAGTGCAACGGTGCTGGAGCCGTGCCACCGGCCACCTTAGCCGAGTTCACACTCGGCTCGGGTTCTCAAGACTTCTATGACGTCAGCCTCGTTGACGGTTATAACTTACCAATGATCGTTGAGGGGAGTGGGGGATCGGGTGCGTGCGCGTCCACTGGGTGCGTTACGGACCTCAACCGTAGGTGCCCTGCCGAGCTCAAAGTCGACGGCGCGTGCAAGAGCGCGTGTGATGCATTTGGAAGTCCGGAGTATTGTTGCAACGGCGCGTACGGTTCACCGTCTACATGCAAGCCGTCCATGTACTCGCAGTTGTTTAAGTCAGCGTGTCCGAAATCATATAGTTACGCTTACGATGACGCTTCAAGTACGTTTACGTGTATAGGTGCTGATTATACGATCACATTTTGCGCTTCTCTTACTTCAAGCCTAAAATCTTCAAGAGATTCGGGGTCGGGGTCGGGGTCAGATTCCGGGTCGAACCCAATAGGTTCTTCATTGCTAGCTGATTTGGCCAGCGGAGATTCAACCAAAACCCAACCTTCCATTTCCATAGCTCTTCAAAGTATGTTGATTTTGGTCATCTCTTTGGCTCTCACATTTCGACAGTTACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thaumatin

Protein Analysis

304

Amino Acids

31.27

Weight (kDa)

4.71

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thaumatin PF00314 29 - 235 2.1e-84 Thaumatin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 399
AasI GACNNNNNNGTC 1 cut(s) 205
AatII GACGTC 1 cut(s) 381
AccB1I GGYRCC 1 cut(s) 480
AccI GTMKAC 2 cut(s) 501, 578
AccII CGCG 5 cut(s) 183, 446, 509, 521, 563
AccIII TCCGGA 1 cut(s) 541
AciI CCGC 3 cut(s) 181, 282, 813
AclWI GGATC 1 cut(s) 439
AcoI YGGCCR 3 cut(s) 107, 327, 807
AcsI RAATTY 1 cut(s) 114
AcyI GRCGYC 1 cut(s) 378
AdeI CACNNNGTG 1 cut(s) 456
AfaI GTAC 4 cut(s) 238, 566, 599, 664
AflIII ACRYGT 1 cut(s) 671
AgsI TTSAA 5 cut(s) 660, 720, 735, 822, 857
AjnI CCWGG 1 cut(s) 189
AjuI GAANNNNNNNTTGG 2 cut(s) 772, 804
AluBI AGCT 6 cut(s) 142, 163, 295, 493, 800, 851
AluI AGCT 6 cut(s) 142, 163, 295, 493, 800, 851
Alw21I GWGCWC 2 cut(s) 297, 495
AlwI GGATC 1 cut(s) 439
Ama87I CYCGRG 3 cut(s) 286, 296, 355
Aor13HI TCCGGA 1 cut(s) 541
AoxI GGCC 6 cut(s) 66, 107, 192, 223, 327, 807
ApeKI GCWGC 1 cut(s) 132
ApoI RAATTY 1 cut(s) 114
AspLEI GCGC 6 cut(s) 128, 446, 509, 521, 563, 707
AspS9I GGNCC 5 cut(s) 66, 193, 207, 223, 466
AsuC2I CCSGG 3 cut(s) 111, 274, 769
AsuHPI GGTGA 2 cut(s) 19, 564
AsuNHI GCTAGC 1 cut(s) 796
AvaI CYCGRG 3 cut(s) 286, 296, 355
AvaII GGWCC 2 cut(s) 207, 466
BaeGI GKGCMC 1 cut(s) 485
BalI TGGCCA 1 cut(s) 809
BanI GGYRCC 1 cut(s) 480
BanII GRGCYC 2 cut(s) 297, 495
Bbv12I GWGCWC 2 cut(s) 297, 495
BbvI GCAGC 1 cut(s) 144
BceAI ACGGC 4 cut(s) 302, 520, 574, 574
BcgI CGANNNNNNTGC 2 cut(s) 139, 173
BciT130I CCWGG 1 cut(s) 191
BcnI CCSGG 3 cut(s) 111, 274, 769
BfaI CTAG 2 cut(s) 119, 797
BfoI RGCGCY 1 cut(s) 129
BglI GCCNNNNNGGC 1 cut(s) 231
BisI GCNGC 2 cut(s) 133, 283
BlsI GCNGC 2 cut(s) 134, 284
Bme1390I CCNGG 4 cut(s) 111, 191, 274, 769
Bme18I GGWCC 2 cut(s) 207, 466
BmeT110I CYCGRG 3 cut(s) 286, 296, 355
BmgT120I GGNCC 5 cut(s) 66, 193, 207, 223, 466
BmiI GGNNCC 5 cut(s) 67, 195, 224, 316, 482
BmrFI CCNGG 4 cut(s) 111, 191, 274, 769
BmrI ACTGGG 1 cut(s) 462
BmsI GCATC 1 cut(s) 517
BmtI GCTAGC 1 cut(s) 800
BmuI ACTGGG 1 cut(s) 462
BpmI CTGGAG 1 cut(s) 333
Bpu10I CCTNAGC 1 cut(s) 334
BpuEI CTTGAG 1 cut(s) 348
BpuMI CCSGG 3 cut(s) 111, 274, 769
BsaAI YACGTR 1 cut(s) 672
BsaHI GRCGYC 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 273
BsaWI WCCGGW 2 cut(s) 209, 541
Bse118I RCCGGY 5 cut(s) 128, 152, 200, 230, 325
Bse1I ACTGG 1 cut(s) 457
Bse3DI GCAATG 1 cut(s) 791
BseAI TCCGGA 1 cut(s) 541
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 1 cut(s) 273
BseMI GCAATG 1 cut(s) 791
BseNI ACTGG 1 cut(s) 457
BseSI GKGCMC 1 cut(s) 485
BseXI GCAGC 1 cut(s) 144
Bsh1236I CGCG 5 cut(s) 183, 446, 509, 521, 563
Bsh1285I CGRYCG 3 cut(s) 201, 214, 273
BshFI GGCC 6 cut(s) 68, 109, 194, 225, 329, 809
BshNI GGYRCC 1 cut(s) 480
BsiEI CGRYCG 3 cut(s) 201, 214, 273
BsiHKAI GWGCWC 2 cut(s) 297, 495
BsiHKCI CYCGRG 3 cut(s) 286, 296, 355
BsiWI CGTACG 1 cut(s) 564
BslFI GGGAC 1 cut(s) 290
BsmFI GGGAC 1 cut(s) 290
BsnI GGCC 6 cut(s) 68, 109, 194, 225, 329, 809
BsoBI CYCGRG 3 cut(s) 286, 296, 355
Bsp1286I GDGCHC 3 cut(s) 297, 485, 495
Bsp13I TCCGGA 1 cut(s) 541
Bsp143I GATC 3 cut(s) 411, 431, 693
BspACI CCGC 3 cut(s) 181, 282, 813
BspANI GGCC 6 cut(s) 68, 109, 194, 225, 329, 809
BspEI TCCGGA 1 cut(s) 541
BspFNI CGCG 5 cut(s) 183, 446, 509, 521, 563
BspLI GGNNCC 5 cut(s) 67, 195, 224, 316, 482
BspOI GCTAGC 1 cut(s) 800
BspPI GGATC 1 cut(s) 439
BspQI GCTCTTC 1 cut(s) 858
BspT107I GGYRCC 1 cut(s) 480
BsrDI GCAATG 1 cut(s) 791
BsrFI RCCGGY 5 cut(s) 128, 152, 200, 230, 325
BsrI ACTGG 1 cut(s) 457
BssAI RCCGGY 5 cut(s) 128, 152, 200, 230, 325
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 3 cut(s) 411, 431, 693
BssNI GRCGYC 1 cut(s) 378
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 8 cut(s) 103, 248, 308, 395, 476, 569, 576, 903
Bst6I CTCTTC 1 cut(s) 858
BstACI GRCGYC 1 cut(s) 378
BstBAI YACGTR 1 cut(s) 672
BstC8I GCNNGC 8 cut(s) 124, 130, 293, 442, 511, 587, 798, 811
BstDEI CTNAG 1 cut(s) 334
BstFNI CGCG 5 cut(s) 183, 446, 509, 521, 563
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 6 cut(s) 128, 446, 509, 521, 563, 707
BstKTI GATC 3 cut(s) 414, 434, 696
BstMBI GATC 3 cut(s) 411, 431, 693
BstMCI CGRYCG 3 cut(s) 201, 214, 273
BstMWI GCNNNNNNNGC 8 cut(s) 132, 180, 222, 231, 288, 335, 527, 806
BstNI CCWGG 1 cut(s) 191
BstNSI RCATGY 1 cut(s) 585
BstSCI CCNGG 4 cut(s) 109, 189, 272, 767
BstSLI GKGCMC 1 cut(s) 485
BstUI CGCG 5 cut(s) 183, 446, 509, 521, 563
BstV1I GCAGC 1 cut(s) 144
BsuRI GGCC 6 cut(s) 68, 109, 194, 225, 329, 809
BtsIMutI CAGTG 1 cut(s) 450
Cac8I GCNNGC 8 cut(s) 124, 130, 293, 442, 511, 587, 798, 811
CfoI GCGC 6 cut(s) 128, 446, 509, 521, 563, 707
Cfr10I RCCGGY 5 cut(s) 128, 152, 200, 230, 325
Cfr13I GGNCC 5 cut(s) 66, 193, 207, 223, 466
CseI GACGC 2 cut(s) 435, 662
Csp6I GTAC 4 cut(s) 237, 565, 598, 663
CviAII CATG 2 cut(s) 582, 595
CviQI GTAC 4 cut(s) 237, 565, 598, 663
DdeI CTNAG 1 cut(s) 334
DpnI GATC 3 cut(s) 413, 433, 695
DpnII GATC 3 cut(s) 411, 431, 693
DraIII CACNNNGTG 1 cut(s) 456
DrdI GACNNNNNNGTC 1 cut(s) 205
DseDI GACNNNNNNGTC 1 cut(s) 205
EaeI YGGCCR 3 cut(s) 107, 327, 807
Eam1104I CTCTTC 1 cut(s) 858
EarI CTCTTC 1 cut(s) 858
Ecl136II GAGCTC 2 cut(s) 295, 493
Eco24I GRGCYC 2 cut(s) 297, 495
Eco47I GGWCC 2 cut(s) 207, 466
Eco53kI GAGCTC 2 cut(s) 295, 493
Eco88I CYCGRG 3 cut(s) 286, 296, 355
EcoICRI GAGCTC 2 cut(s) 295, 493
EcoO109I RGGNCCY 1 cut(s) 193
EcoRI GAATTC 1 cut(s) 114
EcoRII CCWGG 1 cut(s) 189
EcoT22I ATGCAT 1 cut(s) 532
EcoT38I GRGCYC 2 cut(s) 297, 495
FaeI CATG 2 cut(s) 585, 598
FalI AAGNNNNNCTT 2 cut(s) 80, 112
FaqI GGGAC 1 cut(s) 290
FatI CATG 2 cut(s) 581, 594
FauI CCCGC 1 cut(s) 188
FblI GTMKAC 2 cut(s) 501, 578
Fnu4HI GCNGC 2 cut(s) 133, 283
FriOI GRGCYC 2 cut(s) 297, 495
Fsp4HI GCNGC 2 cut(s) 133, 283
FspBI CTAG 2 cut(s) 119, 797
GlaI GCGC 6 cut(s) 127, 445, 508, 520, 562, 706
GluI GCNGC 2 cut(s) 133, 283
GsuI CTGGAG 1 cut(s) 333
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 6 cut(s) 68, 109, 194, 225, 329, 809
HgaI GACGC 2 cut(s) 435, 662
HhaI GCGC 6 cut(s) 128, 446, 509, 521, 563, 707
Hin1I GRCGYC 1 cut(s) 378
Hin1II CATG 2 cut(s) 585, 598
Hin6I GCGC 6 cut(s) 126, 444, 507, 519, 561, 705
HinP1I GCGC 6 cut(s) 126, 444, 507, 519, 561, 705
HincII GTYRAC 2 cut(s) 391, 502
HindII GTYRAC 2 cut(s) 391, 502
HinfI GANTC 3 cut(s) 740, 764, 818
HphI GGTGA 2 cut(s) 19, 564
Hpy166II GTNNAC 8 cut(s) 81, 345, 391, 450, 502, 572, 579, 669
Hpy188I TCNGA 2 cut(s) 627, 763
Hpy188III TCNNGA 3 cut(s) 365, 542, 735
Hpy8I GTNNAC 8 cut(s) 81, 345, 391, 450, 502, 572, 579, 669
Hpy99I CGWCG 1 cut(s) 506
HpyAV CCTTC 3 cut(s) 40, 47, 846
HpyCH4III ACNGT 8 cut(s) 103, 248, 308, 395, 476, 569, 576, 903
HpyCH4IV ACGT 4 cut(s) 77, 378, 665, 671
HpyCH4V TGCA 5 cut(s) 303, 513, 530, 555, 585
HpyF10VI GCNNNNNNNGC 8 cut(s) 132, 180, 222, 231, 288, 335, 527, 806
HpyF3I CTNAG 1 cut(s) 334
HpySE526I ACGT 4 cut(s) 77, 378, 665, 671
Hsp92I GRCGYC 1 cut(s) 378
Hsp92II CATG 2 cut(s) 585, 598
HspAI GCGC 6 cut(s) 126, 444, 507, 519, 561, 705
Kpn2I TCCGGA 1 cut(s) 541
KroI GCCGGC 1 cut(s) 128
KroNI GCCGGC 1 cut(s) 130
Kzo9I GATC 3 cut(s) 411, 431, 693
LguI GCTCTTC 1 cut(s) 858
LmnI GCTCC 1 cut(s) 314
Lsp1109I GCAGC 1 cut(s) 144
LweI GCATC 1 cut(s) 517
MaeI CTAG 2 cut(s) 119, 797
MaeII ACGT 4 cut(s) 77, 378, 665, 671
MaeIII GTNAC 3 cut(s) 460, 638, 903
MalI GATC 3 cut(s) 413, 433, 695
MboI GATC 3 cut(s) 411, 431, 693
MboII GAAGA 4 cut(s) 29, 723, 780, 845
MhlI GDGCHC 3 cut(s) 297, 485, 495
MlsI TGGCCA 1 cut(s) 809
MluCI AATT 3 cut(s) 12, 42, 114
MluNI TGGCCA 1 cut(s) 809
MnlI CCTC 3 cut(s) 395, 412, 479
Mox20I TGGCCA 1 cut(s) 809
Mph1103I ATGCAT 1 cut(s) 532
MroI TCCGGA 1 cut(s) 541
MroNI GCCGGC 1 cut(s) 128
MscI TGGCCA 1 cut(s) 809
MseI TTAA 1 cut(s) 612
Msp20I TGGCCA 1 cut(s) 809
MspA1I CMGCKG 1 cut(s) 813
MspR9I CCNGG 4 cut(s) 111, 191, 274, 769
MvaI CCWGG 1 cut(s) 191
MvnI CGCG 5 cut(s) 183, 446, 509, 521, 563
MwoI GCNNNNNNNGC 8 cut(s) 132, 180, 222, 231, 288, 335, 527, 806
NaeI GCCGGC 1 cut(s) 130
NciI CCSGG 3 cut(s) 111, 274, 769
NdeII GATC 3 cut(s) 411, 431, 693
NgoMIV GCCGGC 1 cut(s) 128
NheI GCTAGC 1 cut(s) 796
NlaIII CATG 2 cut(s) 585, 598
NlaIV GGNNCC 5 cut(s) 67, 195, 224, 316, 482
NmeAIII GCCGAG 3 cut(s) 330, 364, 514
NsiI ATGCAT 1 cut(s) 532
NspI RCATGY 1 cut(s) 585
PaeR7I CTCGAG 1 cut(s) 296
PciSI GCTCTTC 1 cut(s) 858
PcsI WCGNNNNNNNCGW 1 cut(s) 384
PdiI GCCGGC 1 cut(s) 130
PfeI GAWTC 3 cut(s) 740, 764, 818
Pfl23II CGTACG 1 cut(s) 564
PkrI GCNGC 2 cut(s) 134, 284
Ppu21I YACGTR 1 cut(s) 672
PsiI TTATAA 1 cut(s) 399
Psp124BI GAGCTC 2 cut(s) 297, 495
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspLI CGTACG 1 cut(s) 564
PspN4I GGNNCC 5 cut(s) 67, 195, 224, 316, 482
PspPI GGNCC 5 cut(s) 66, 193, 207, 223, 466
PspXI VCTCGAGB 1 cut(s) 296
PsrI GAACNNNNNNTAC 2 cut(s) 220, 252
RsaI GTAC 4 cut(s) 238, 566, 599, 664
RsaNI GTAC 4 cut(s) 237, 565, 598, 663
SacI GAGCTC 2 cut(s) 297, 495
SalI GTCGAC 1 cut(s) 500
SapI GCTCTTC 1 cut(s) 858
SaqAI TTAA 1 cut(s) 612
SatI GCNGC 2 cut(s) 133, 283
Sau3AI GATC 3 cut(s) 411, 431, 693
Sau96I GGNCC 5 cut(s) 66, 193, 207, 223, 466
ScrFI CCNGG 4 cut(s) 111, 191, 274, 769
SduI GDGCHC 3 cut(s) 297, 485, 495
SfaNI GCATC 1 cut(s) 517
Sfr274I CTCGAG 1 cut(s) 296
SinI GGWCC 2 cut(s) 207, 466
SlaI CTCGAG 1 cut(s) 296
SmlI CTYRAG 2 cut(s) 296, 363
SmoI CTYRAG 2 cut(s) 296, 363
Sse9I AATT 3 cut(s) 12, 42, 114
SsiI CCGC 3 cut(s) 181, 282, 813
SspMI CTAG 2 cut(s) 119, 797
SstI GAGCTC 2 cut(s) 297, 495
StyD4I CCNGG 4 cut(s) 109, 189, 272, 767
TaaI ACNGT 8 cut(s) 103, 248, 308, 395, 476, 569, 576, 903
TaiI ACGT 4 cut(s) 80, 381, 668, 674
TaqI TCGA 7 cut(s) 144, 159, 269, 297, 501, 773, 898
TasI AATT 3 cut(s) 12, 42, 114
TatI WGTACW 1 cut(s) 597
TauI GCSGC 1 cut(s) 285
TfiI GAWTC 3 cut(s) 740, 764, 818
Tru1I TTAA 1 cut(s) 612
Tru9I TTAA 1 cut(s) 612
TscAI CASTG 1 cut(s) 457
TseI GCWGC 1 cut(s) 132
TspDTI ATGAA 2 cut(s) 22, 780
TspGWI ACGGA 1 cut(s) 479
TspRI CASTG 1 cut(s) 457
VpaK11BI GGWCC 2 cut(s) 207, 466
XapI RAATTY 1 cut(s) 114
XceI RCATGY 1 cut(s) 585
XhoI CTCGAG 1 cut(s) 296
XmiI GTMKAC 2 cut(s) 501, 578
XspI CTAG 2 cut(s) 119, 797
ZraI GACGTC 1 cut(s) 379
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.