MD10G1121900.v1.1

Thaumatin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003319
Physical Location & Seq
Forward (+)
19917729 .. 19920484
2756 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1121900.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGATCCCTTCTTTACATCCTTCCCTTCTCTCACCGTCATCCTTCTTCTCTTACTAGCCATGGCTTCCACAGGTGTATTAGCGACGACATTCACATTCGTCAACAGATGCGACTACACAGTGTGGCCCGGCATTCTCGCTAACGCCAACAGTCCTAGTCTCGACTCCACCGGATTCGAGCTTCCCAAACAAACCGCCCGCACTTTCCAAGCCCCAACCGGCTGGTCCGGTCGTTTCTGGGCCCGAACCGGCTGCAGTTTCGACGGATCCGGTTCCGGGTCCTGCACCACCGGCGACTGTGGCTCTGGCCAAGTCGAATGCAACGGCGCCGGAGCCTCCCCGCCTGCGACGCTTGCCGAGTTTACTCTCGGCACCGGGGGACAGGACTTTTATGACGTTAGCCTCGTAGACGGGTACAACTTGCCCGTTTTCGTCGAGGGGACCGGCGGGTCGGGTCAGTGCGCCTCGACCGGGTGCTCCTCCGATCTGAACAGGATGTGCCCGACTGAGTTGAGAGTCGGAGACGGCGACGCTTGTAAGAGCGCGTGCGAGGCGTTTGGGACTCCCGAGTACTGTTGCAGCGGCGCGTATGCTACACCCAACACTTGTAGCCCGTCGGTTTACTCGCAGATGTTTAAGGCGGCGTGCCCCAAGTCGTATAGCTACGCCTACTACGATGCTACGAGTACGTTTACTTGCACCGGTGCTGATTATACGGTGACGTTTTGCCCCTCTTCCCCAAGTCAGAAATCTTCTAGAGATTCAACCACACCAATGACAGCAACACCATCACAAGGGGCTGCTACTGCCGGGTCGGATCCCGGGTTCACTTATTCAGATTCAGGTGCCGGCTCAGGCGGTGCAGTGTCCGGTTCTGGTACAGGTGTGGATACGGGTACTGGGGCAGGTTCCGGCACCGGGGCTGGCGAAGCAATGCTGGCTGACGGGTCATGGCTAGCTGGTTTGGCCATGGGAGACTCACCTAAGACAGTGCCTCTCCCCACTCTACACTCAACACTCACTACCCCAGCAATTTTATGTATCGTCTTGTCCTATCTTTACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thaumatin

Protein Analysis

356

Amino Acids

36.0

Weight (kDa)

4.21

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thaumatin PF00314 35 - 244 2.2e-82 Thaumatin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 448
Acc36I ACCTGC 1 cut(s) 896
AccB1I GGYRCC 4 cut(s) 326, 371, 845, 914
AccI GTMKAC 1 cut(s) 408
AccII CGCG 2 cut(s) 545, 587
AciI CCGC 7 cut(s) 195, 199, 341, 447, 582, 641, 858
AclWI GGATC 5 cut(s) 12, 261, 274, 812, 825
AcoI YGGCCR 2 cut(s) 307, 966
AcyI GRCGYC 1 cut(s) 327
AdeI CACNNNGTG 1 cut(s) 123
AfaI GTAC 5 cut(s) 416, 572, 688, 880, 898
AfiI CCNNNNNNNGG 4 cut(s) 276, 471, 794, 918
AgeI ACCGGT 1 cut(s) 701
AgsI TTSAA 1 cut(s) 765
AloI GAACNNNNNNTCC 2 cut(s) 809, 841
AluBI AGCT 3 cut(s) 181, 663, 959
AluI AGCT 3 cut(s) 181, 663, 959
Alw21I GWGCWC 1 cut(s) 479
Alw26I GTCTC 3 cut(s) 164, 516, 969
AlwI GGATC 5 cut(s) 12, 261, 274, 812, 825
Ama87I CYCGRG 2 cut(s) 566, 821
AoxI GGCC 4 cut(s) 125, 240, 307, 966
ApaI GGGCCC 1 cut(s) 244
ApeKI GCWGC 3 cut(s) 252, 579, 800
AsiGI ACCGGT 1 cut(s) 701
AspLEI GCGC 4 cut(s) 329, 464, 545, 587
AspS9I GGNCC 6 cut(s) 126, 225, 240, 241, 279, 441
AsuC2I CCSGG 8 cut(s) 129, 277, 376, 472, 811, 822, 823, 919
AsuHPI GGTGA 3 cut(s) 25, 730, 972
AsuNHI GCTAGC 1 cut(s) 955
AvaI CYCGRG 2 cut(s) 566, 821
AvaII GGWCC 3 cut(s) 225, 279, 441
BaeGI GKGCMC 3 cut(s) 244, 503, 650
BaeI ACNNNNGTAYC 2 cut(s) 870, 903
BalI TGGCCA 2 cut(s) 309, 968
BamHI GGATCC 3 cut(s) 4, 266, 817
BanI GGYRCC 4 cut(s) 326, 371, 845, 914
BanII GRGCYC 1 cut(s) 244
Bbv12I GWGCWC 1 cut(s) 479
BbvI GCAGC 3 cut(s) 239, 591, 787
BccI CCATC 1 cut(s) 796
BceAI ACGGC 2 cut(s) 340, 541
BcgI CGANNNNNNTGC 2 cut(s) 234, 268
BciVI GTATCC 1 cut(s) 883
BcnI CCSGG 8 cut(s) 129, 277, 376, 472, 811, 822, 823, 919
BcoDI GTCTC 3 cut(s) 164, 516, 969
BfaI CTAG 4 cut(s) 56, 156, 756, 956
BfmI CTRYAG 1 cut(s) 253
BfoI RGCGCY 1 cut(s) 330
BfuAI ACCTGC 1 cut(s) 896
BfuI GTATCC 1 cut(s) 883
BisI GCNGC 5 cut(s) 253, 580, 583, 642, 801
BlsI GCNGC 5 cut(s) 254, 581, 584, 643, 802
BmcAI AGTACT 1 cut(s) 572
Bme1390I CCNGG 8 cut(s) 129, 277, 376, 472, 811, 822, 823, 919
Bme18I GGWCC 3 cut(s) 225, 279, 441
BmeT110I CYCGRG 2 cut(s) 566, 821
BmgT120I GGNCC 6 cut(s) 126, 225, 240, 241, 279, 441
BmrFI CCNGG 8 cut(s) 129, 277, 376, 472, 811, 822, 823, 919
BmrI ACTGGG 1 cut(s) 909
BmsI GCATC 2 cut(s) 98, 667
BmtI GCTAGC 1 cut(s) 959
BmuI ACTGGG 1 cut(s) 909
Bpu10I CCTNAGC 1 cut(s) 853
BpuMI CCSGG 8 cut(s) 129, 277, 376, 472, 811, 822, 823, 919
BsaHI GRCGYC 1 cut(s) 327
BsaJI CCNNGG 5 cut(s) 60, 375, 821, 918, 969
BsaWI WCCGGW 5 cut(s) 170, 227, 269, 701, 869
Bsc4I CCNNNNNNNGG 4 cut(s) 276, 471, 794, 918
Bse118I RCCGGY 6 cut(s) 218, 248, 290, 443, 701, 848
Bse1I ACTGG 1 cut(s) 904
Bse3DI GCAATG 1 cut(s) 939
BseDI CCNNGG 5 cut(s) 60, 375, 821, 918, 969
BseGI GGATG 3 cut(s) 17, 39, 501
BseLI CCNNNNNNNGG 4 cut(s) 276, 471, 794, 918
BseMI GCAATG 1 cut(s) 939
BseMII CTCAG 2 cut(s) 498, 867
BseNI ACTGG 1 cut(s) 904
BseRI GAGGAG 1 cut(s) 469
BseSI GKGCMC 3 cut(s) 244, 503, 650
BseXI GCAGC 3 cut(s) 239, 591, 787
BseYI CCCAGC 1 cut(s) 1027
BsgI GTGCAG 2 cut(s) 268, 882
Bsh1236I CGCG 2 cut(s) 545, 587
Bsh1285I CGRYCG 2 cut(s) 232, 471
BshFI GGCC 4 cut(s) 127, 242, 309, 968
BshNI GGYRCC 4 cut(s) 326, 371, 845, 914
BshTI ACCGGT 1 cut(s) 701
BsiEI CGRYCG 2 cut(s) 232, 471
BsiHKAI GWGCWC 1 cut(s) 479
BsiHKCI CYCGRG 2 cut(s) 566, 821
BslFI GGGAC 3 cut(s) 393, 454, 574
BslI CCNNNNNNNGG 4 cut(s) 276, 471, 794, 918
BsmAI GTCTC 3 cut(s) 164, 516, 969
BsmBI CGTCTC 1 cut(s) 516
BsmFI GGGAC 3 cut(s) 393, 454, 574
BsmI GAATGC 2 cut(s) 132, 323
BsnI GGCC 4 cut(s) 127, 242, 309, 968
BsoBI CYCGRG 2 cut(s) 566, 821
Bsp120I GGGCCC 1 cut(s) 240
Bsp1286I GDGCHC 4 cut(s) 244, 479, 503, 650
Bsp143I GATC 4 cut(s) 4, 266, 484, 817
Bsp19I CCATGG 2 cut(s) 60, 969
BspACI CCGC 7 cut(s) 195, 199, 341, 447, 582, 641, 858
BspANI GGCC 4 cut(s) 127, 242, 309, 968
BspCNI CTCAG 2 cut(s) 499, 866
BspFNI CGCG 2 cut(s) 545, 587
BspMAI CTGCAG 1 cut(s) 257
BspMI ACCTGC 1 cut(s) 896
BspOI GCTAGC 1 cut(s) 959
BspPI GGATC 5 cut(s) 12, 261, 274, 812, 825
BspT107I GGYRCC 4 cut(s) 326, 371, 845, 914
BsrDI GCAATG 1 cut(s) 939
BsrFI RCCGGY 6 cut(s) 218, 248, 290, 443, 701, 848
BsrI ACTGG 1 cut(s) 904
BssAI RCCGGY 6 cut(s) 218, 248, 290, 443, 701, 848
BssECI CCNNGG 5 cut(s) 60, 375, 821, 918, 969
BssMI GATC 4 cut(s) 4, 266, 484, 817
BssNI GRCGYC 1 cut(s) 327
BssT1I CCWWGG 2 cut(s) 60, 969
Bst4CI ACNGT 7 cut(s) 37, 121, 152, 299, 575, 718, 991
Bst6I CTCTTC 1 cut(s) 739
BstACI GRCGYC 1 cut(s) 327
BstC8I GCNNGC 9 cut(s) 199, 345, 354, 547, 646, 850, 925, 939, 957
BstDEI CTNAG 3 cut(s) 507, 853, 984
BstDSI CCRYGG 2 cut(s) 60, 969
BstF5I GGATG 3 cut(s) 17, 39, 501
BstFNI CGCG 2 cut(s) 545, 587
BstH2I RGCGCY 1 cut(s) 330
BstHHI GCGC 4 cut(s) 329, 464, 545, 587
BstKTI GATC 4 cut(s) 7, 269, 487, 820
BstMAI GTCTC 3 cut(s) 164, 516, 969
BstMBI GATC 4 cut(s) 4, 266, 484, 817
BstMCI CGRYCG 2 cut(s) 232, 471
BstMWI GCNNNNNNNGC 9 cut(s) 291, 300, 349, 353, 551, 806, 929, 938, 965
BstSCI CCNGG 8 cut(s) 127, 275, 374, 470, 809, 820, 821, 917
BstSFI CTRYAG 1 cut(s) 253
BstSLI GKGCMC 3 cut(s) 244, 503, 650
BstUI CGCG 2 cut(s) 545, 587
BstV1I GCAGC 3 cut(s) 239, 591, 787
BstX2I RGATCY 3 cut(s) 4, 266, 817
BstXI CCANNNNNNTGG 1 cut(s) 222
BstYI RGATCY 3 cut(s) 4, 266, 817
BsuI GTATCC 1 cut(s) 883
BsuRI GGCC 4 cut(s) 127, 242, 309, 968
BtgI CCRYGG 2 cut(s) 60, 969
BtsCI GGATG 3 cut(s) 17, 39, 501
BtsI GCAGTG 1 cut(s) 870
BtsIMutI CAGTG 4 cut(s) 126, 464, 870, 996
BveI ACCTGC 1 cut(s) 896
Cac8I GCNNGC 9 cut(s) 199, 345, 354, 547, 646, 850, 925, 939, 957
CfoI GCGC 4 cut(s) 329, 464, 545, 587
Cfr10I RCCGGY 6 cut(s) 218, 248, 290, 443, 701, 848
Cfr13I GGNCC 6 cut(s) 126, 225, 240, 241, 279, 441
Cfr9I CCCGGG 1 cut(s) 821
CseI GACGC 2 cut(s) 358, 539
Csp6I GTAC 5 cut(s) 415, 571, 687, 879, 897
CspAI ACCGGT 1 cut(s) 701
CviAII CATG 3 cut(s) 61, 951, 970
CviQI GTAC 5 cut(s) 415, 571, 687, 879, 897
DdeI CTNAG 3 cut(s) 507, 853, 984
DinI GGCGCC 1 cut(s) 328
DpnI GATC 4 cut(s) 6, 268, 486, 819
DpnII GATC 4 cut(s) 4, 266, 484, 817
DraIII CACNNNGTG 1 cut(s) 123
DrdI GACNNNNNNGTC 1 cut(s) 448
DseDI GACNNNNNNGTC 1 cut(s) 448
EaeI YGGCCR 2 cut(s) 307, 966
Eam1104I CTCTTC 1 cut(s) 739
EarI CTCTTC 1 cut(s) 739
Eco130I CCWWGG 2 cut(s) 60, 969
Eco24I GRGCYC 1 cut(s) 244
Eco47I GGWCC 3 cut(s) 225, 279, 441
Eco88I CYCGRG 2 cut(s) 566, 821
EcoO109I RGGNCCY 1 cut(s) 279
EcoT14I CCWWGG 2 cut(s) 60, 969
EcoT38I GRGCYC 1 cut(s) 244
EgeI GGCGCC 1 cut(s) 328
EheI GGCGCC 1 cut(s) 328
ErhI CCWWGG 2 cut(s) 60, 969
Esp3I CGTCTC 1 cut(s) 516
FaeI CATG 3 cut(s) 64, 954, 973
FaiI YATR 8 cut(s) 62, 393, 591, 660, 714, 952, 971, 1039
FaqI GGGAC 3 cut(s) 393, 454, 574
FatI CATG 3 cut(s) 60, 950, 969
FauI CCCGC 3 cut(s) 206, 348, 440
FblI GTMKAC 1 cut(s) 408
Fnu4HI GCNGC 5 cut(s) 253, 580, 583, 642, 801
FokI GGATG 3 cut(s) 4, 26, 508
FriOI GRGCYC 1 cut(s) 244
Fsp4HI GCNGC 5 cut(s) 253, 580, 583, 642, 801
FspBI CTAG 4 cut(s) 56, 156, 756, 956
GlaI GCGC 4 cut(s) 328, 463, 544, 586
GluI GCNGC 5 cut(s) 253, 580, 583, 642, 801
GsaI CCCAGC 1 cut(s) 1031
HaeII RGCGCY 1 cut(s) 330
HaeIII GGCC 4 cut(s) 127, 242, 309, 968
HgaI GACGC 2 cut(s) 358, 539
HhaI GCGC 4 cut(s) 329, 464, 545, 587
Hin1I GRCGYC 1 cut(s) 327
Hin1II CATG 3 cut(s) 64, 954, 973
Hin6I GCGC 4 cut(s) 327, 462, 543, 585
HinP1I GCGC 4 cut(s) 327, 462, 543, 585
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HinfI GANTC 7 cut(s) 164, 174, 516, 562, 761, 839, 977
HphI GGTGA 3 cut(s) 25, 730, 972
Hpy166II GTNNAC 6 cut(s) 103, 363, 409, 622, 693, 828
Hpy188I TCNGA 6 cut(s) 484, 489, 521, 747, 817, 838
Hpy188III TCNNGA 3 cut(s) 161, 566, 756
Hpy8I GTNNAC 6 cut(s) 103, 363, 409, 622, 693, 828
Hpy99I CGWCG 6 cut(s) 88, 266, 352, 437, 533, 619
HpyAV CCTTC 4 cut(s) 19, 31, 36, 53
HpyCH4III ACNGT 7 cut(s) 37, 121, 152, 299, 575, 718, 991
HpyCH4IV ACGT 3 cut(s) 396, 689, 722
HpyCH4V TGCA 6 cut(s) 255, 285, 321, 579, 699, 863
HpyF10VI GCNNNNNNNGC 9 cut(s) 291, 300, 349, 353, 551, 806, 929, 938, 965
HpyF3I CTNAG 3 cut(s) 507, 853, 984
HpySE526I ACGT 3 cut(s) 396, 689, 722
Hsp92I GRCGYC 1 cut(s) 327
Hsp92II CATG 3 cut(s) 64, 954, 973
HspAI GCGC 4 cut(s) 327, 462, 543, 585
KasI GGCGCC 1 cut(s) 326
KroI GCCGGC 1 cut(s) 848
KroNI GCCGGC 1 cut(s) 850
Kzo9I GATC 4 cut(s) 4, 266, 484, 817
LmnI GCTCC 2 cut(s) 332, 482
Lsp1109I GCAGC 3 cut(s) 239, 591, 787
LweI GCATC 2 cut(s) 98, 667
MaeI CTAG 4 cut(s) 56, 156, 756, 956
MaeII ACGT 3 cut(s) 396, 689, 722
MaeIII GTNAC 1 cut(s) 718
MalI GATC 4 cut(s) 6, 268, 486, 819
MboI GATC 4 cut(s) 4, 266, 484, 817
MboII GAAGA 3 cut(s) 38, 726, 744
MflI RGATCY 3 cut(s) 4, 266, 817
MhlI GDGCHC 4 cut(s) 244, 479, 503, 650
MlsI TGGCCA 2 cut(s) 309, 968
MluCI AATT 1 cut(s) 1032
MluNI TGGCCA 2 cut(s) 309, 968
Mly113I GGCGCC 1 cut(s) 327
MlyI GAGTC 4 cut(s) 158, 525, 556, 971
MmeI TCCRAC 2 cut(s) 499, 795
MnlI CCTC 8 cut(s) 346, 413, 430, 475, 490, 544, 742, 1005
Mox20I TGGCCA 2 cut(s) 309, 968
MroNI GCCGGC 1 cut(s) 848
MscI TGGCCA 2 cut(s) 309, 968
MseI TTAA 1 cut(s) 636
MslI CAYNNNNRTG 1 cut(s) 773
Msp20I TGGCCA 2 cut(s) 309, 968
MspA1I CMGCKG 1 cut(s) 582
MspR9I CCNGG 8 cut(s) 129, 277, 376, 472, 811, 822, 823, 919
Mva1269I GAATGC 2 cut(s) 132, 323
MvnI CGCG 2 cut(s) 545, 587
MwoI GCNNNNNNNGC 9 cut(s) 291, 300, 349, 353, 551, 806, 929, 938, 965
NaeI GCCGGC 1 cut(s) 850
NarI GGCGCC 1 cut(s) 327
NciI CCSGG 8 cut(s) 129, 277, 376, 472, 811, 822, 823, 919
NcoI CCATGG 2 cut(s) 60, 969
NdeII GATC 4 cut(s) 4, 266, 484, 817
NgoMIV GCCGGC 1 cut(s) 848
NheI GCTAGC 1 cut(s) 955
NlaIII CATG 3 cut(s) 64, 954, 973
NmeAIII GCCGAG 2 cut(s) 348, 382
NmuCI GTSAC 1 cut(s) 718
PcsI WCGNNNNNNNCGW 3 cut(s) 402, 525, 672
PctI GAATGC 2 cut(s) 132, 323
PdiI GCCGGC 1 cut(s) 850
PfeI GAWTC 3 cut(s) 174, 761, 839
PinAI ACCGGT 1 cut(s) 701
PkrI GCNGC 5 cut(s) 254, 581, 584, 643, 802
PleI GAGTC 4 cut(s) 158, 524, 556, 971
PluTI GGCGCC 1 cut(s) 330
PpsI GAGTC 4 cut(s) 158, 524, 556, 971
PpuMI RGGWCCY 1 cut(s) 279
Psp5II RGGWCCY 1 cut(s) 279
PspFI CCCAGC 1 cut(s) 1027
PspOMI GGGCCC 1 cut(s) 240
PspPI GGNCC 6 cut(s) 126, 225, 240, 241, 279, 441
PspPPI RGGWCCY 1 cut(s) 279
PstI CTGCAG 1 cut(s) 257
PsuI RGATCY 3 cut(s) 4, 266, 817
RsaI GTAC 5 cut(s) 416, 572, 688, 880, 898
RsaNI GTAC 5 cut(s) 415, 571, 687, 879, 897
RseI CAYNNNNRTG 1 cut(s) 773
SaqAI TTAA 1 cut(s) 636
SatI GCNGC 5 cut(s) 253, 580, 583, 642, 801
Sau3AI GATC 4 cut(s) 4, 266, 484, 817
Sau96I GGNCC 6 cut(s) 126, 225, 240, 241, 279, 441
ScaI AGTACT 1 cut(s) 572
SchI GAGTC 4 cut(s) 158, 525, 556, 971
ScrFI CCNGG 8 cut(s) 129, 277, 376, 472, 811, 822, 823, 919
SduI GDGCHC 4 cut(s) 244, 479, 503, 650
SfaNI GCATC 2 cut(s) 98, 667
SfcI CTRYAG 1 cut(s) 253
SfoI GGCGCC 1 cut(s) 328
SgrAI CRCCGGYG 2 cut(s) 290, 701
SinI GGWCC 3 cut(s) 225, 279, 441
SmaI CCCGGG 1 cut(s) 823
SmiMI CAYNNNNRTG 1 cut(s) 773
Sse9I AATT 1 cut(s) 1032
SsiI CCGC 7 cut(s) 195, 199, 341, 447, 582, 641, 858
SspDI GGCGCC 1 cut(s) 326
SspMI CTAG 4 cut(s) 56, 156, 756, 956
StyD4I CCNGG 8 cut(s) 127, 275, 374, 470, 809, 820, 821, 917
StyI CCWWGG 2 cut(s) 60, 969
TaaI ACNGT 7 cut(s) 37, 121, 152, 299, 575, 718, 991
TaiI ACGT 3 cut(s) 399, 692, 725
TaqI TCGA 6 cut(s) 162, 177, 261, 315, 435, 467
TasI AATT 1 cut(s) 1032
TatI WGTACW 1 cut(s) 570
TauI GCSGC 2 cut(s) 585, 644
TfiI GAWTC 3 cut(s) 174, 761, 839
Tru1I TTAA 1 cut(s) 636
Tru9I TTAA 1 cut(s) 636
TscAI CASTG 4 cut(s) 126, 464, 870, 996
TseFI GTSAC 1 cut(s) 718
TseI GCWGC 3 cut(s) 252, 579, 800
Tsp45I GTSAC 1 cut(s) 718
TspGWI ACGGA 1 cut(s) 279
TspMI CCCGGG 1 cut(s) 821
TspRI CASTG 4 cut(s) 126, 464, 870, 996
VpaK11BI GGWCC 3 cut(s) 225, 279, 441
XbaI TCTAGA 1 cut(s) 755
XmaI CCCGGG 1 cut(s) 821
XmiI GTMKAC 1 cut(s) 408
XspI CTAG 4 cut(s) 56, 156, 756, 956
ZrmI AGTACT 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.