Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0005662

Physical Location & Seq

Genomic Coordinates Reverse (-)

26060888 .. 26061868

Gene Structure 981 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1223200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 981 bp

ATGTCGGAAACAGCAGCAGCACCGCCGCCGCCTCTGCCAATACTACCTCACCGCCAATCCTCCTCCTCCTTCAAAACCCTAACCCCGAGCCTCTTCATCATCATTGTCATCCTCACAGCCACACTCGTGGCCTCAATCTGCCTCTGTCTCATCCTCCGCCACCTCAACCGCCGATGCATCCGTCGCCTAGCGCCTTCCCCCGCCATCTCATCCTCCGATTCTCGCCGCATATCCACTCGCCGAGTCAATCCGGTGACCTTGCCTACCCTCCCTCTCTTCACCTTCTCCTCCATCACTCGCCGATCCTCCACCACCATATCCGCCGACTGCGCCGTCTGCCTGTCGAAATTCGAGCCCAGCGACGAGCTCCGTCTCCTCCCGCTCTGCTGCCACGCCTTCCACGCCGTCTGCATCGACACGTGGCTCCAGTCGCAGCAGACTTGCCCACTCTGTCGCTCCTCGATCGCCGCTTCCGACGCCGAGCTCATGACGCTCGCCTCCTCCAATGAAGGCGCAGCCACCACAAACGGTGGCAGCTTCCGCCTCGAAATCGGAAACGTCAGCCGCCGCAACAACAACCAGACGACGACGACGACGACTGCGGCTTCGTCCAATCGGCACTCCTACTCCGTCGGCTCCTTCGATTACATCGTGGACGAGGAGGCGTCGGAAGTGAGGCTGAGCAATGCTGAGCGAGAGAAAGACGAAGTCATCTTGCCGGTGACAGAGCCGGAGCCGCCGATTCCGGCTCCGTTCCTCGCCGCGGAGGTGGGCTCAGGGAGGTCGAGCTGGCTCAAGGACTACATTGATCGGCTGTCCAGCACCCTCTCGTCCCGAGATATCTCATTTCGGAGCTCTGGACGATTCTTCACCGGAAGCAGCCGTCGGAGCGAGGTGTCCGTGGCCGGCGATTGGAGCTTGGAAACAGAGCGCGTCGGAGAAGAGATATCGGAGATGTTCCGGTGGCTTGCAGGGGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

327

Amino Acids

35.62

Weight (kDa)

6.95

Isoelectric Point (pI)

76.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	109 - 152	2.1e-13	Ring finger domain
zf-RING_11	PF17123	109 - 138	1e-09	RING-like zinc finger
zf-C3HC4_2	PF13923	110 - 151	1e-05	Zinc finger, C3HC4 type (RING finger)
zf-ANAPC11	PF12861	118 - 156	4.4e-06	Anaphase-promoting complex subunit 11 RING-H2 finger
zf-rbx1	PF12678	123 - 152	1.1e-07	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005662)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G60220
fragaria_vesca	FvH4_7g08860
malus_domestica	MD02G1223200.v1.1 MD07G1091500.v1.1
prunus_persica	Prupe.2G116700_v2.0.a1
pyrus_communis	pycom07g07480 pycom15g32160
rosa_chinensis	RchiOBHm_Chr1g0344271 RchiOBHm_Chr7g0197351
rosa_laevigata	RLG00000003768 RLG00000015102 RLG00000019331 RLG00000025671 RLG00000028912
rosa_multiflora	Rmu_sc0000019.1_g000012 Rmu_sc0000338.1_g000018 Rmu_sc0003703.1_g000004 Rmu_sc0005991.1_g000007 Rmu_sc0016684.1_g000002 Rmu_sc0016685.1_g000001
rosa_roxburghii	Rroxscaffold_4G00309960
rosa_rugosa	Rorug01G0172700
rosa_samantha	Rh1AG188600 Rh1BG155100 Rh1CG174400 Rh2CG262100 Rh3BG248600
rosa_wichuraiana	Rw1G015540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	332
AccBSI	CCGCTC	1 cut(s)	382
AccII	CGCG	2 cut(s)	764, 933
AclWI	GGATC	1 cut(s)	297
AcoI	YGGCCR	1 cut(s)	903
AcsI	RAATTY	1 cut(s)	347
AcvI	CACGTG	1 cut(s)	420
AcyI	GRCGYC	2 cut(s)	477, 665
AfiI	CCNNNNNNNGG	2 cut(s)	763, 912
AflIII	ACRYGT	1 cut(s)	417
AgsI	TTSAA	1 cut(s)	73
AleI	CACNNNNGTG	1 cut(s)	125
AluBI	AGCT	6 cut(s)	367, 484, 537, 789, 855, 918
AluI	AGCT	6 cut(s)	367, 484, 537, 789, 855, 918
Alw21I	GWGCWC	3 cut(s)	369, 486, 857
Alw26I	GTCTC	2 cut(s)	152, 377
AlwI	GGATC	1 cut(s)	297
Ama87I	CYCGRG	2 cut(s)	85, 834
AoxI	GGCC	2 cut(s)	129, 903
ApeKI	GCWGC	7 cut(s)	14, 17, 387, 433, 515, 534, 879
ApoI	RAATTY	1 cut(s)	347
ArsI	GACNNNNNNTTYG	2 cut(s)	589, 621
AspLEI	GCGC	4 cut(s)	193, 332, 515, 933
AsuHPI	GGTGA	5 cut(s)	41, 265, 271, 733, 862
AvaI	CYCGRG	2 cut(s)	85, 834
BanII	GRGCYC	5 cut(s)	357, 369, 486, 776, 857
BauI	CACGAG	1 cut(s)	125
BbrPI	CACGTG	1 cut(s)	420
Bbv12I	GWGCWC	3 cut(s)	369, 486, 857
BbvI	GCAGC	7 cut(s)	26, 29, 374, 445, 527, 546, 891
BccI	CCATC	2 cut(s)	212, 299
BceAI	ACGGC	3 cut(s)	317, 389, 867
BcoDI	GTCTC	2 cut(s)	152, 377
BfaI	CTAG	1 cut(s)	188
BfoI	RGCGCY	1 cut(s)	194
BlpI	GCTNAGC	2 cut(s)	680, 690
BmeT110I	CYCGRG	2 cut(s)	85, 834
BmiI	GGNNCC	4 cut(s)	425, 637, 735, 750
BmsI	GCATC	3 cut(s)	164, 186, 420
BplI	GAGNNNNNCTC	2 cut(s)	758, 790
BpmI	CTGGAG	1 cut(s)	410
Bpu10I	CCTNAGC	1 cut(s)	775
Bpu1102I	GCTNAGC	2 cut(s)	680, 690
BpuEI	CTTGAG	1 cut(s)	779
BsaAI	YACGTR	1 cut(s)	420
BsaHI	GRCGYC	2 cut(s)	477, 665
BsaJI	CCNNGG	2 cut(s)	762, 900
BsaWI	WCCGGW	3 cut(s)	250, 872, 960
BsaXI	ACNNNNNCTCC	2 cut(s)	611, 641
Bsc4I	CCNNNNNNNGG	2 cut(s)	763, 912
Bse118I	RCCGGY	2 cut(s)	718, 905
Bse1I	ACTGG	1 cut(s)	427
Bse3DI	GCAATG	1 cut(s)	691
BseDI	CCNNGG	2 cut(s)	762, 900
BseGI	GGATG	4 cut(s)	108, 150, 177, 209
BseLI	CCNNNNNNNGG	2 cut(s)	763, 912
BseMI	GCAATG	1 cut(s)	691
BseMII	CTCAG	3 cut(s)	671, 681, 789
BseNI	ACTGG	1 cut(s)	427
BseRI	GAGGAG	7 cut(s)	52, 55, 277, 365, 448, 490, 674
BseXI	GCAGC	7 cut(s)	26, 29, 374, 445, 527, 546, 891
BseYI	CCCAGC	1 cut(s)	356
Bsh1236I	CGCG	2 cut(s)	764, 933
Bsh1285I	CGRYCG	1 cut(s)	465
BshFI	GGCC	2 cut(s)	131, 905
BsiEI	CGRYCG	1 cut(s)	465
BsiHKAI	GWGCWC	3 cut(s)	369, 486, 857
BsiHKCI	CYCGRG	2 cut(s)	85, 834
BsiSI	CCGG	7 cut(s)	251, 719, 731, 746, 873, 906, 961
BslFI	GGGAC	1 cut(s)	817
BslI	CCNNNNNNNGG	2 cut(s)	763, 912
BsmAI	GTCTC	2 cut(s)	152, 377
BsmBI	CGTCTC	1 cut(s)	377
BsmFI	GGGAC	1 cut(s)	817
BsnI	GGCC	2 cut(s)	131, 905
BsoBI	CYCGRG	2 cut(s)	85, 834
Bsp1286I	GDGCHC	5 cut(s)	357, 369, 486, 776, 857
Bsp143I	GATC	3 cut(s)	302, 462, 808
Bsp1720I	GCTNAGC	2 cut(s)	680, 690
BspANI	GGCC	2 cut(s)	131, 905
BspCNI	CTCAG	3 cut(s)	672, 682, 788
BspFNI	CGCG	2 cut(s)	764, 933
BspHI	TCATGA	1 cut(s)	486
BspLI	GGNNCC	4 cut(s)	425, 637, 735, 750
BspPI	GGATC	1 cut(s)	297
BsrBI	CCGCTC	1 cut(s)	382
BsrDI	GCAATG	1 cut(s)	691
BsrFI	RCCGGY	2 cut(s)	718, 905
BsrI	ACTGG	1 cut(s)	427
BssAI	RCCGGY	2 cut(s)	718, 905
BssECI	CCNNGG	2 cut(s)	762, 900
BssMI	GATC	3 cut(s)	302, 462, 808
BssNI	GRCGYC	2 cut(s)	477, 665
BssSI	CACGAG	1 cut(s)	125
Bst2BI	CACGAG	1 cut(s)	125
Bst4CI	ACNGT	1 cut(s)	530
Bst6I	CTCTTC	3 cut(s)	98, 281, 936
BstACI	GRCGYC	2 cut(s)	477, 665
BstBAI	YACGTR	1 cut(s)	420
BstC8I	GCNNGC	4 cut(s)	495, 791, 907, 969
BstDEI	CTNAG	3 cut(s)	680, 690, 775
BstDSI	CCRYGG	2 cut(s)	762, 900
BstEII	GGTNACC	1 cut(s)	253
BstF5I	GGATG	4 cut(s)	108, 150, 177, 209
BstFNI	CGCG	2 cut(s)	764, 933
BstH2I	RGCGCY	1 cut(s)	194
BstHHI	GCGC	4 cut(s)	193, 332, 515, 933
BstKTI	GATC	3 cut(s)	305, 465, 811
BstMAI	GTCTC	2 cut(s)	152, 377
BstMBI	GATC	3 cut(s)	302, 462, 808
BstMCI	CGRYCG	1 cut(s)	465
BstPI	GGTNACC	1 cut(s)	253
BstUI	CGCG	2 cut(s)	764, 933
BstV1I	GCAGC	7 cut(s)	26, 29, 374, 445, 527, 546, 891
BstXI	CCANNNNNNTGG	1 cut(s)	127
BsuRI	GGCC	2 cut(s)	131, 905
BtgI	CCRYGG	2 cut(s)	762, 900
BtsCI	GGATG	4 cut(s)	108, 150, 177, 209
Cac8I	GCNNGC	4 cut(s)	495, 791, 907, 969
CciI	TCATGA	1 cut(s)	486
CfoI	GCGC	4 cut(s)	193, 332, 515, 933
Cfr10I	RCCGGY	2 cut(s)	718, 905
Cfr42I	CCGCGG	1 cut(s)	765
CseI	GACGC	4 cut(s)	485, 499, 654, 922
CviAII	CATG	1 cut(s)	487
DdeI	CTNAG	3 cut(s)	680, 690, 775
DpnI	GATC	3 cut(s)	304, 464, 810
DpnII	GATC	3 cut(s)	302, 462, 808
DrdI	GACNNNNNNGTC	1 cut(s)	332
DseDI	GACNNNNNNGTC	1 cut(s)	332
EaeI	YGGCCR	1 cut(s)	903
Eam1104I	CTCTTC	3 cut(s)	98, 281, 936
EarI	CTCTTC	3 cut(s)	98, 281, 936
EciI	GGCGGA	3 cut(s)	146, 310, 530
Ecl136II	GAGCTC	3 cut(s)	367, 484, 855
Eco24I	GRGCYC	5 cut(s)	357, 369, 486, 776, 857
Eco32I	GATATC	2 cut(s)	841, 948
Eco53kI	GAGCTC	3 cut(s)	367, 484, 855
Eco72I	CACGTG	1 cut(s)	420
Eco88I	CYCGRG	2 cut(s)	85, 834
Eco91I	GGTNACC	1 cut(s)	253
EcoICRI	GAGCTC	3 cut(s)	367, 484, 855
EcoO65I	GGTNACC	1 cut(s)	253
EcoRV	GATATC	2 cut(s)	841, 948
EcoT22I	ATGCAT	1 cut(s)	179
EcoT38I	GRGCYC	5 cut(s)	357, 369, 486, 776, 857
Esp3I	CGTCTC	1 cut(s)	377
FaeI	CATG	1 cut(s)	490
FaiI	YATR	4 cut(s)	230, 317, 488, 979
FaqI	GGGAC	1 cut(s)	817
FatI	CATG	1 cut(s)	486
FauI	CCCGC	2 cut(s)	208, 387
FokI	GGATG	4 cut(s)	95, 137, 164, 196
FriOI	GRGCYC	5 cut(s)	357, 369, 486, 776, 857
FspBI	CTAG	1 cut(s)	188
GlaI	GCGC	4 cut(s)	192, 331, 514, 932
GsaI	CCCAGC	1 cut(s)	360
GsuI	CTGGAG	1 cut(s)	410
HaeII	RGCGCY	1 cut(s)	194
HaeIII	GGCC	2 cut(s)	131, 905
HapII	CCGG	7 cut(s)	251, 719, 731, 746, 873, 906, 961
HgaI	GACGC	4 cut(s)	485, 499, 654, 922
HhaI	GCGC	4 cut(s)	193, 332, 515, 933
Hin1I	GRCGYC	2 cut(s)	477, 665
Hin1II	CATG	1 cut(s)	490
Hin6I	GCGC	4 cut(s)	191, 330, 513, 931
HinP1I	GCGC	4 cut(s)	191, 330, 513, 931
HinfI	GANTC	4 cut(s)	218, 243, 742, 864
HpaII	CCGG	7 cut(s)	251, 719, 731, 746, 873, 906, 961
HphI	GGTGA	5 cut(s)	41, 265, 271, 733, 862
Hpy166II	GTNNAC	1 cut(s)	655
Hpy188I	TCNGA	9 cut(s)	7, 217, 475, 554, 670, 852, 888, 938, 952
Hpy188III	TCNNGA	3 cut(s)	487, 834, 858
Hpy8I	GTNNAC	1 cut(s)	655
HpyAV	CCTTC	6 cut(s)	79, 204, 292, 406, 503, 649
HpyCH4III	ACNGT	1 cut(s)	530
HpyCH4IV	ACGT	2 cut(s)	419, 558
HpyCH4V	TGCA	3 cut(s)	177, 411, 971
HpyF3I	CTNAG	3 cut(s)	680, 690, 775
HpySE526I	ACGT	2 cut(s)	419, 558
Hsp92I	GRCGYC	2 cut(s)	477, 665
Hsp92II	CATG	1 cut(s)	490
HspAI	GCGC	4 cut(s)	191, 330, 513, 931
KroI	GCCGGC	1 cut(s)	905
KroNI	GCCGGC	1 cut(s)	907
KspI	CCGCGG	1 cut(s)	765
Kzo9I	GATC	3 cut(s)	302, 462, 808
LmnI	GCTCC	9 cut(s)	372, 429, 461, 641, 733, 754, 852, 888, 915
Lsp1109I	GCAGC	7 cut(s)	26, 29, 374, 445, 527, 546, 891
LweI	GCATC	3 cut(s)	164, 186, 420
MaeI	CTAG	1 cut(s)	188
MaeII	ACGT	2 cut(s)	419, 558
MaeIII	GTNAC	2 cut(s)	253, 721
MalI	GATC	3 cut(s)	304, 464, 810
MbiI	CCGCTC	1 cut(s)	382
MboI	GATC	3 cut(s)	302, 462, 808
MboII	GAAGA	4 cut(s)	85, 268, 859, 953
MhlI	GDGCHC	5 cut(s)	357, 369, 486, 776, 857
MluCI	AATT	1 cut(s)	347
MlyI	GAGTC	1 cut(s)	252
MmeI	TCCRAC	4 cut(s)	498, 648, 866, 916
Mph1103I	ATGCAT	1 cut(s)	179
MroNI	GCCGGC	1 cut(s)	905
MslI	CAYNNNNRTG	1 cut(s)	125
MspA1I	CMGCKG	1 cut(s)	764
MspI	CCGG	7 cut(s)	251, 719, 731, 746, 873, 906, 961
MvnI	CGCG	2 cut(s)	764, 933
NaeI	GCCGGC	1 cut(s)	907
NdeII	GATC	3 cut(s)	302, 462, 808
NgoMIV	GCCGGC	1 cut(s)	905
NlaIII	CATG	1 cut(s)	490
NlaIV	GGNNCC	4 cut(s)	425, 637, 735, 750
NmeAIII	GCCGAG	2 cut(s)	266, 505
NmuCI	GTSAC	2 cut(s)	253, 721
NsiI	ATGCAT	1 cut(s)	179
OliI	CACNNNNGTG	1 cut(s)	125
PagI	TCATGA	1 cut(s)	486
PcsI	WCGNNNNNNNCGW	6 cut(s)	357, 471, 590, 593, 639, 648
PdiI	GCCGGC	1 cut(s)	907
PfeI	GAWTC	3 cut(s)	218, 742, 864
PflFI	GACNNNGTC	1 cut(s)	707
Ple19I	CGATCG	1 cut(s)	465
PleI	GAGTC	1 cut(s)	251
PmaCI	CACGTG	1 cut(s)	420
PmlI	CACGTG	1 cut(s)	420
PpsI	GAGTC	1 cut(s)	251
Ppu21I	YACGTR	1 cut(s)	420
Psp124BI	GAGCTC	3 cut(s)	369, 486, 857
PspCI	CACGTG	1 cut(s)	420
PspEI	GGTNACC	1 cut(s)	253
PspFI	CCCAGC	1 cut(s)	356
PspN4I	GGNNCC	4 cut(s)	425, 637, 735, 750
PsyI	GACNNNGTC	1 cut(s)	707
PvuI	CGATCG	1 cut(s)	465
RseI	CAYNNNNRTG	1 cut(s)	125
SacI	GAGCTC	3 cut(s)	369, 486, 857
SacII	CCGCGG	1 cut(s)	765
Sau3AI	GATC	3 cut(s)	302, 462, 808
SchI	GAGTC	1 cut(s)	252
SduI	GDGCHC	5 cut(s)	357, 369, 486, 776, 857
SfaNI	GCATC	3 cut(s)	164, 186, 420
Sfr303I	CCGCGG	1 cut(s)	765
SgrBI	CCGCGG	1 cut(s)	765
SmiMI	CAYNNNNRTG	1 cut(s)	125
SmlI	CTYRAG	1 cut(s)	794
SmoI	CTYRAG	1 cut(s)	794
Sse9I	AATT	1 cut(s)	347
SspMI	CTAG	1 cut(s)	188
SstI	GAGCTC	3 cut(s)	369, 486, 857
TaaI	ACNGT	1 cut(s)	530
TaiI	ACGT	2 cut(s)	422, 561
TaqI	TCGA	7 cut(s)	344, 351, 414, 461, 546, 642, 785
TasI	AATT	1 cut(s)	347
TauI	GCSGC	9 cut(s)	28, 31, 228, 470, 567, 570, 605, 739, 764
TfiI	GAWTC	3 cut(s)	218, 742, 864
TseFI	GTSAC	2 cut(s)	253, 721
TseI	GCWGC	7 cut(s)	14, 17, 387, 433, 515, 534, 879
Tsp45I	GTSAC	2 cut(s)	253, 721
TspDTI	ATGAA	2 cut(s)	85, 522
TspGWI	ACGGA	5 cut(s)	170, 359, 619, 741, 889
Tth111I	GACNNNGTC	1 cut(s)	707
XapI	RAATTY	1 cut(s)	347
XspI	CTAG	1 cut(s)	188
Zsp2I	ATGCAT	1 cut(s)	179

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.