MD02G1298000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003892
Physical Location & Seq
Forward (+)
35291758 .. 35295265
3508 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1298000.v1.1.491

Sequence Viewer

Length: 492 bp
ATGTCGTTGGTGGACTACCCATCTTCAGACGACGACGTATCGGAGGAGGAGGTTAGGGAAAACAAGGAAAATGAGCCACAGCAACAATTACCCAGAGACGATCCACGTCCTCACACTCAATTGGTTGTTTCATCATACCAGCAGCCGGAAAGTTCTGCCCCTGCATCAGCTCCTTCAATCCAGCAACTTCCTGATGCTTCGATGCTCTTGAATTCTCCTGTTTCCTCATCCAATATGTTGAGTGGCGGCGACCACTCTTCTCGTGTTGCAGCTGCAATGGCGGAGAGTTCATCACGCAAGAGGGAGAATGCATTTGCTTCCTCTGATCCTCGCAATAAAGTTCCTAGAGGTAACTTGCCTCATTCCAAGAATGTTCCAGATACAGTTGGTGGTCTGCTGGTTCCGCCTCAGCTTCGTGGAAGGAGCAACATTGTTACAGAAGATGTAGGAAAGCTATTTGTCAAAAAGCAGGCTGACCCTTCACCAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

164

Amino Acids

17.61

Weight (kDa)

5.37

Isoelectric Point (pI)

74.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 246, 281, 404
AclWI GGATC 2 cut(s) 95, 320
AcsI RAATTY 1 cut(s) 211
AcuI CTGAAG 1 cut(s) 9
AfiI CCNNNNNNNGG 1 cut(s) 145
AgsI TTSAA 2 cut(s) 177, 211
AjiI CACGTC 1 cut(s) 107
AluBI AGCT 4 cut(s) 170, 272, 412, 454
AluI AGCT 4 cut(s) 170, 272, 412, 454
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 2 cut(s) 95, 320
ApeKI GCWGC 3 cut(s) 142, 269, 272
ApoI RAATTY 1 cut(s) 211
AsuHPI GGTGA 1 cut(s) 474
BauI CACGAG 1 cut(s) 261
BbvCI CCTCAGC 1 cut(s) 408
BbvI GCAGC 3 cut(s) 154, 259, 281
BccI CCATC 1 cut(s) 28
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 1 cut(s) 345
BisI GCNGC 4 cut(s) 143, 247, 270, 273
BlsI GCNGC 4 cut(s) 144, 248, 271, 274
BmgBI CACGTC 1 cut(s) 107
BmiI GGNNCC 1 cut(s) 402
BmsI GCATC 3 cut(s) 173, 184, 192
Bpu10I CCTNAGC 1 cut(s) 408
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse3DI GCAATG 1 cut(s) 282
BseGI GGATG 1 cut(s) 227
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMI GCAATG 1 cut(s) 282
BseMII CTCAG 1 cut(s) 422
BseRI GAGGAG 2 cut(s) 59, 62
BseXI GCAGC 3 cut(s) 154, 259, 281
BsiSI CCGG 1 cut(s) 146
BslI CCNNNNNNNGG 1 cut(s) 145
BsmAI GTCTC 1 cut(s) 90
BsmBI CGTCTC 1 cut(s) 90
BsmI GAATGC 1 cut(s) 313
Bsp143I GATC 2 cut(s) 100, 325
BspACI CCGC 3 cut(s) 246, 281, 404
BspCNI CTCAG 1 cut(s) 421
BspLI GGNNCC 1 cut(s) 402
BspPI GGATC 2 cut(s) 95, 320
BsrDI GCAATG 1 cut(s) 282
BssMI GATC 2 cut(s) 100, 325
BssSI CACGAG 1 cut(s) 261
Bst2BI CACGAG 1 cut(s) 261
Bst4CI ACNGT 1 cut(s) 385
Bst6I CTCTTC 1 cut(s) 262
BstC8I GCNNGC 1 cut(s) 471
BstDEI CTNAG 1 cut(s) 408
BstF5I GGATG 1 cut(s) 227
BstKTI GATC 2 cut(s) 103, 328
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 2 cut(s) 100, 325
BstMWI GCNNNNNNNGC 2 cut(s) 278, 403
BstV1I GCAGC 3 cut(s) 154, 259, 281
BtrI CACGTC 1 cut(s) 107
BtsCI GGATG 1 cut(s) 227
Cac8I GCNNGC 1 cut(s) 471
CviJI RGCY 7 cut(s) 76, 145, 170, 272, 412, 454, 473
CviKI_1 RGCY 7 cut(s) 76, 145, 170, 272, 412, 454, 473
DdeI CTNAG 1 cut(s) 408
DpnI GATC 2 cut(s) 102, 327
DpnII GATC 2 cut(s) 100, 325
Eam1104I CTCTTC 1 cut(s) 262
EarI CTCTTC 1 cut(s) 262
EciI GGCGGA 2 cut(s) 296, 393
Eco57I CTGAAG 1 cut(s) 9
EcoRI GAATTC 1 cut(s) 211
EcoT22I ATGCAT 1 cut(s) 313
Esp3I CGTCTC 1 cut(s) 90
FaiI YATR 2 cut(s) 136, 236
Fnu4HI GCNGC 4 cut(s) 143, 247, 270, 273
FokI GGATG 1 cut(s) 214
Fsp4HI GCNGC 4 cut(s) 143, 247, 270, 273
FspBI CTAG 1 cut(s) 345
GluI GCNGC 4 cut(s) 143, 247, 270, 273
HapII CCGG 1 cut(s) 146
HpaII CCGG 1 cut(s) 146
HphI GGTGA 1 cut(s) 474
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 3 cut(s) 28, 43, 325
Hpy188III TCNNGA 3 cut(s) 191, 208, 377
Hpy8I GTNNAC 1 cut(s) 13
Hpy99I CGWCG 2 cut(s) 35, 38
HpyAV CCTTC 3 cut(s) 183, 414, 489
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4IV ACGT 2 cut(s) 36, 106
HpyCH4V TGCA 4 cut(s) 164, 269, 275, 311
HpyF10VI GCNNNNNNNGC 2 cut(s) 278, 403
HpyF3I CTNAG 1 cut(s) 408
HpySE526I ACGT 2 cut(s) 36, 106
Kzo9I GATC 2 cut(s) 100, 325
LmnI GCTCC 2 cut(s) 175, 423
Lsp1109I GCAGC 3 cut(s) 154, 259, 281
LweI GCATC 3 cut(s) 173, 184, 192
MaeI CTAG 1 cut(s) 345
MaeII ACGT 2 cut(s) 36, 106
MaeIII GTNAC 2 cut(s) 350, 433
MalI GATC 2 cut(s) 102, 327
MboI GATC 2 cut(s) 100, 325
MboII GAAGA 3 cut(s) 15, 249, 452
MfeI CAATTG 1 cut(s) 119
MluCI AATT 3 cut(s) 86, 119, 211
Mph1103I ATGCAT 1 cut(s) 313
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 1 cut(s) 146
MunI CAATTG 1 cut(s) 119
Mva1269I GAATGC 1 cut(s) 313
MwoI GCNNNNNNNGC 2 cut(s) 278, 403
NdeII GATC 2 cut(s) 100, 325
NlaIV GGNNCC 1 cut(s) 402
NsiI ATGCAT 1 cut(s) 313
PctI GAATGC 1 cut(s) 313
PkrI GCNGC 4 cut(s) 144, 248, 271, 274
PspN4I GGNNCC 1 cut(s) 402
PvuII CAGCTG 1 cut(s) 272
SatI GCNGC 4 cut(s) 143, 247, 270, 273
Sau3AI GATC 2 cut(s) 100, 325
SetI ASST 8 cut(s) 39, 54, 109, 172, 274, 352, 414, 456
SfaNI GCATC 3 cut(s) 173, 184, 192
Sse9I AATT 3 cut(s) 86, 119, 211
SsiI CCGC 3 cut(s) 246, 281, 404
SspMI CTAG 1 cut(s) 345
TaaI ACNGT 1 cut(s) 385
TaiI ACGT 2 cut(s) 39, 109
TaqI TCGA 1 cut(s) 200
TasI AATT 3 cut(s) 86, 119, 211
TauI GCSGC 1 cut(s) 249
TseI GCWGC 3 cut(s) 142, 269, 272
TspDTI ATGAA 2 cut(s) 120, 279
XapI RAATTY 1 cut(s) 211
XspI CTAG 1 cut(s) 345
Zsp2I ATGCAT 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.