Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002528

Physical Location & Seq

Genomic Coordinates Reverse (-)

1913097 .. 1918407

Gene Structure 5311 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1023500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2346 bp

ATGGCTAGAAGGTATGGTAGTGAGTCAAGCGATAATGATATCAGAGGTGAATATCGTCGTCATAGTAGTAGAACTAAGCGTCATGATGAGCGTTGTAGAGATGTGGACAGGGAAAAGTCCACTGAAAGGGATCCCAGAGGCAGAGAAAGTTCTGATGAAGAAGGGGAGTTGATAGAGAGAGACAGGGGCAGCCAAAGGAATGAAAGAAAGTCTGAGAAAGGGCGTAGAGAAAGGGAAAAAGGTAGTTACCAGGATGAAAGGGAGAAATTACATAGGCGTGAGAGTGAACGTAGAGGGGTAGATACTGATGGTGGTCATAGAAGTAGGGGCCGAGATTCGCATCACTATTCAGAGCGTAAGGATGAAAGGGATGAGCATAGAACATTAGAACGGAAAAAGGATAAAAGAGATGATGATCAGAGAAGGTTTCATAAAAACGAGGAAGATGAAAGACGAGAAGGAGAAGATAGAGGTTTGAGGGAAAATAATGGAAATTCAATACCTCAAGGACGGTTGCCAGAAGGAAAAATGAACGCTGATGTGGCAAATACAGGAAAGAGTGGTGGGGTTTACATTCCCCCATTTAAGTTGGCCCGTATGATGAAAGAGGTTGAAGATAAAAGCAGTGTTCAGTATCAGCGACTGACATGGGATGCCCTTCGAAAGAGCATTAATGGTCTTGTGAACAAGGTCAATGCTGCAAACATAAAAAATATAATTCCTGAAATTTTTGCTGAGAATCTGATTCGTGGAAGGGGCCTCTTTTGCCGTTCGTGTATGAAATCACAGATGGCATCTCCAGGTTTCACTGATGTGTTTGCAGCCTTGGTTGCTGTTGTCAACACGAAGTTTCCTGAGGTTGGCGAACTTTTGTTGAGAAGAATTGTCTTACAGCTTAAGAGAGCATTTAAGCGGAATGACAAGCCGCAACTACTAGCTGCTGTTAAATTTATAGCTCATCTGGTCAACCAGCAAGTGGCTCATGAGATTATTGCCTTAGAATTGCTCACGGTTCTGTTGGAGAACCCTACGGATGACAGTGTTGAAGTTGCTGTTGGCTTTGTCACTGAGTGTGGATCATTACTGCAGGACCTTTCGCCTAAAGGGTTGCACGGCATCTTTGAGCGTTTTCGTGGAATTCTTCATGAGGGAGAAATAGACAAGAGAGTTCAATATCTGATTGAAAACTTATTTGCAATACGGAAAGCGAAGTTTCAGGGGCACCCAGCTGTTCGTCCAGAACTGGACCTTGTAGAGCAGGAAGACCAGTTGACACATGAAATTTCTCTTGAAGAGAAAATAGATTCAGAAGTTACCCTTGATCTTTTCAAGGCCGATCCTGATTTCCTTGAGAACGAAAACCGCTATGAAGAACTGAAGAAAACCATACTTGGTGAGGATTCTGAAGATGAAGAAGGTTCTGATGTAGCGTCAGATGGTGACGACAACGACGACGATGATGATGAAGAATCTGAGGAAGAGGATGAGGAGCAGATGCAGATAAGAGACGAGACAGAGACAAATCTTGTGAATCTTAGAAGAACTATCTATCTTACAATCATGTCCAGTGTAGATTTTGAGGAGGCGGGTCATAAGCTCCTGAAGATTAAACTTGAGCCTGGTCAAGAGATGGAACTATGTGTCATGCTTTTGGAATGTTGCAGTCAAGAAAGAACCTACCTCCGGTATTATGGTCTCTTGGGGCAAAGATTCTGCATGATCAACAAAGTCCACCAGGAAAATTTTGAGAAATGCTTTGTGCAACAGTATTCCATGATCCACCGGCTTGAGACAAATAAGTTACGGAATGTTGCAAAGTTTTTCGCCCATTTACTCGGCACAGATGCCCTTCCTTGGCATGTGTTGGCCTATATACGATTGACTGAAGAGGACACTACTTCATCTTCTCGTATTTTTATCAAGATCCTTTTCCAGGAATTGTCAGAGCATCTTGGCATCCGTTTGCTAAATGAGCGGCTCACAGATCCAGCAATGCAGGAATCTTATGATTCTATTTTCCCAAAGGATAATCCCAAGAACACCCGCTTTGCCATTAATTTTTTCACATCCATTGGGCTTGGTGGCATCACCGAGAACCTGCGCGAGTACTTGAAGCATATGCCACGACTCATTATGCAACAACAAAAACCAGTGTCTGACTCAGAATCAGATGAAGAATCTGGGAGCTCTAGTTCATCAGATTCGGGAACTAGTTCCGAGTCTGAATCAGAATCAGATTCATCAAGCTCTTCTGAAAGTGAGAGTGATAGGGACAAAAGACAGAAGAAGCGTAGGAGTGAAAGCAAGAGGGATGAAAGACACGAGAAGCGCAGGAGGAGAGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0071006 GO:0071012 GO:0071013 GO:0071704 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MA3 MIF4G

Protein Analysis

782

Amino Acids

90.63

Weight (kDa)

5.46

Isoelectric Point (pI)

58.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MIF4G	PF02854	222 - 403	1.1e-15	MIF4G domain
MA3	PF02847	512 - 618	1.3e-30	MA3 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002528)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G80930
fragaria_vesca	FvH4_3g01450 FvH4_3g43230 FvH4_3g43240 FvH4_3g43240
malus_domestica	MD03G1023500.v1.1 MD11G1028600.v1.1
prunus_persica	Prupe.6G022700_v2.0.a1 Prupe.6G022700_v2.0.a1
pyrus_communis	pycom03g02010 pycom11g02210
rosa_chinensis	RchiOBHm_Chr5g0002291 RchiOBHm_Chr5g0077281 RchiOBHm_Chr5g0077291
rosa_laevigata	RLG00000031014 RLG00000036681 RLG00000036682
rosa_multiflora	Rmu_co8446135.1_g000001 Rmu_sc0000433.1_g000042 Rmu_sc0001492.1_g000008 Rmu_sc0005576.1_g000001 Rmu_sc0006137.1_g000009 Rmu_sc0008383.1_g000001 Rmu_sc0013155.1_g000012 Rmu_ssc0000410.1_g000040
rosa_roxburghii	Rroxscaffold_1G00004680 Rroxscaffold_1G00073650
rosa_rugosa	Rorug04G0394900 Rorug05G0453500
rosa_samantha	Rh5AG017700 Rh5AG508400 Rh5AG508500 Rh5BG020300 Rh5BG529600 Rh5CG019100 Rh5CG019400 Rh5CG554100 Rh5DG018300 Rh5DG542200
rosa_wichuraiana	Rw5G046900 Rw5G046910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2106
AccB1I	GGYRCC	1 cut(s)	1221
AccB7I	CCANNNNNTGG	1 cut(s)	976
AccBSI	CCGCTC	1 cut(s)	1975
AccII	CGCG	1 cut(s)	2103
AciI	CCGC	6 cut(s)	913, 926, 1363, 1586, 1975, 2044
AclWI	GGATC	7 cut(s)	125, 138, 1084, 1331, 1771, 1918, 1979
AcsI	RAATTY	6 cut(s)	493, 726, 947, 1137, 1281, 1741
AcuI	CTGAAG	4 cut(s)	1397, 1425, 1622, 1905
AdeI	CACNNNGTG	1 cut(s)	1071
AfaI	GTAC	1 cut(s)	2108
AfiI	CCNNNNNNNGG	6 cut(s)	126, 860, 976, 1683, 1854, 1933
AflII	CTTAAG	1 cut(s)	896
AgsI	TTSAA	8 cut(s)	498, 614, 1046, 1172, 1184, 1292, 1330, 2113
AhlI	ACTAGT	1 cut(s)	2210
AjnI	CCWGG	5 cut(s)	249, 799, 1618, 1734, 1932
AjuI	GAANNNNNNNTTGG	2 cut(s)	1038, 1070
AleI	CACNNNNGTG	1 cut(s)	812
AloI	GAACNNNNNNTCC	2 cut(s)	2176, 2208
AluBI	AGCT	7 cut(s)	895, 938, 956, 1229, 1597, 2187, 2247
AluI	AGCT	7 cut(s)	895, 938, 956, 1229, 1597, 2187, 2247
Alw21I	GWGCWC	1 cut(s)	2189
Alw26I	GTCTC	6 cut(s)	174, 1500, 1505, 1511, 1700, 1784
AlwI	GGATC	7 cut(s)	125, 138, 1084, 1331, 1771, 1918, 1979
AlwNI	CAGNNNCTG	2 cut(s)	643, 2156
AoxI	GGCC	5 cut(s)	328, 591, 757, 1332, 1866
ApeKI	GCWGC	4 cut(s)	189, 698, 821, 938
ApoI	RAATTY	6 cut(s)	493, 726, 947, 1137, 1281, 1741
AseI	ATTAAT	2 cut(s)	672, 2055
Asp700I	GAANNNNTTC	1 cut(s)	2212
AspLEI	GCGC	2 cut(s)	2103, 2331
AspS9I	GGNCC	5 cut(s)	328, 592, 757, 1090, 1246
AsuHPI	GGTGA	4 cut(s)	59, 1406, 1451, 2080
AsuII	TTCGAA	1 cut(s)	661
AvaII	GGWCC	2 cut(s)	1090, 1246
AxyI	CCTNAGG	1 cut(s)	855
BaeGI	GKGCMC	1 cut(s)	1224
BamHI	GGATCC	1 cut(s)	130
BanI	GGYRCC	1 cut(s)	1221
BanII	GRGCYC	1 cut(s)	2189
BauI	CACGAG	1 cut(s)	2321
BbsI	GAAGAC	1 cut(s)	1269
Bbv12I	GWGCWC	1 cut(s)	2189
BbvI	GCAGC	4 cut(s)	201, 685, 833, 925
BccI	CCATC	4 cut(s)	302, 784, 1430, 1624
BceAI	ACGGC	2 cut(s)	753, 1129
BciT130I	CCWGG	5 cut(s)	251, 801, 1620, 1736, 1934
BclI	TGATCA	2 cut(s)	415, 1719
BcoDI	GTCTC	6 cut(s)	174, 1500, 1505, 1511, 1700, 1784
BcuI	ACTAGT	1 cut(s)	2210
BfaI	CTAG	4 cut(s)	6, 935, 2190, 2211
BfmI	CTRYAG	1 cut(s)	1085
BfrI	CTTAAG	1 cut(s)	896
BfuAI	ACCTGC	1 cut(s)	2106
BisI	GCNGC	6 cut(s)	190, 699, 822, 926, 939, 1976
BlsI	GCNGC	6 cut(s)	191, 700, 823, 927, 940, 1977
BmcAI	AGTACT	1 cut(s)	2108
Bme1390I	CCNGG	5 cut(s)	251, 801, 1620, 1736, 1934
Bme18I	GGWCC	2 cut(s)	1090, 1246
BmgT120I	GGNCC	5 cut(s)	328, 592, 757, 1090, 1246
BmiI	GGNNCC	4 cut(s)	132, 329, 758, 1223
BmrFI	CCNGG	5 cut(s)	251, 801, 1620, 1736, 1934
BmsI	GCATC	9 cut(s)	349, 643, 803, 1125, 1485, 1834, 1957, 1965, 2094
BpiI	GAAGAC	1 cut(s)	1269
BpmI	CTGGAG	1 cut(s)	783
Bpu14I	TTCGAA	1 cut(s)	661
BpuEI	CTTGAG	4 cut(s)	489, 1370, 1634, 1808
BsaBI	GATNNNNATC	2 cut(s)	339, 414
BsaI	GGTCTC	1 cut(s)	1700
BsaJI	CCNNGG	2 cut(s)	825, 1853
BsaWI	WCCGGW	1 cut(s)	1683
BsaXI	ACNNNNNCTCC	2 cut(s)	2176, 2206
Bsc4I	CCNNNNNNNGG	6 cut(s)	126, 860, 976, 1683, 1854, 1933
Bse118I	RCCGGY	1 cut(s)	1782
Bse1I	ACTGG	4 cut(s)	1248, 1267, 1566, 2150
Bse21I	CCTNAGG	1 cut(s)	855
Bse3DI	GCAATG	1 cut(s)	1998
Bse8I	GATNNNNATC	2 cut(s)	339, 414
BseBI	CCWGG	5 cut(s)	251, 801, 1620, 1736, 1934
BseDI	CCNNGG	2 cut(s)	825, 1853
BseGI	GGATG	9 cut(s)	259, 367, 376, 658, 1039, 1489, 1956, 2066, 2317
BseJI	GATNNNNATC	2 cut(s)	339, 414
BseLI	CCNNNNNNNGG	6 cut(s)	126, 860, 976, 1683, 1854, 1933
BseMI	GCAATG	1 cut(s)	1998
BseMII	CTCAG	6 cut(s)	204, 726, 846, 1059, 1464, 2175
BseNI	ACTGG	4 cut(s)	1248, 1267, 1566, 2150
BseRI	GAGGAG	2 cut(s)	1502, 1595
BseSI	GKGCMC	1 cut(s)	1224
BseXI	GCAGC	4 cut(s)	201, 685, 833, 925
BseYI	CCCAGC	1 cut(s)	1225
Bsh1236I	CGCG	1 cut(s)	2103
BshFI	GGCC	5 cut(s)	330, 593, 759, 1334, 1868
BshNI	GGYRCC	1 cut(s)	1221
BsiHKAI	GWGCWC	1 cut(s)	2189
BsiSI	CCGG	2 cut(s)	1684, 1783
BslFI	GGGAC	1 cut(s)	2285
BslI	CCNNNNNNNGG	6 cut(s)	126, 860, 976, 1683, 1854, 1933
BsmAI	GTCTC	6 cut(s)	174, 1500, 1505, 1511, 1700, 1784
BsmBI	CGTCTC	1 cut(s)	1500
BsmFI	GGGAC	1 cut(s)	2285
BsnI	GGCC	5 cut(s)	330, 593, 759, 1334, 1868
Bso31I	GGTCTC	1 cut(s)	1700
Bsp119I	TTCGAA	1 cut(s)	661
Bsp1286I	GDGCHC	2 cut(s)	1224, 2189
Bsp143I	GATC	9 cut(s)	130, 415, 1076, 1321, 1336, 1719, 1776, 1923, 1984
BspACI	CCGC	6 cut(s)	913, 926, 1363, 1586, 1975, 2044
BspANI	GGCC	5 cut(s)	330, 593, 759, 1334, 1868
BspCNI	CTCAG	6 cut(s)	205, 727, 847, 1060, 1465, 2174
BspFNI	CGCG	1 cut(s)	2103
BspHI	TCATGA	3 cut(s)	82, 982, 1144
BspLI	GGNNCC	4 cut(s)	132, 329, 758, 1223
BspMAI	CTGCAG	1 cut(s)	1089
BspMI	ACCTGC	1 cut(s)	2106
BspPI	GGATC	7 cut(s)	125, 138, 1084, 1331, 1771, 1918, 1979
BspQI	GCTCTTC	1 cut(s)	2254
BspT104I	TTCGAA	1 cut(s)	661
BspT107I	GGYRCC	1 cut(s)	1221
BspTI	CTTAAG	1 cut(s)	896
BspTNI	GGTCTC	1 cut(s)	1700
BsrBI	CCGCTC	1 cut(s)	1975
BsrDI	GCAATG	1 cut(s)	1998
BsrFI	RCCGGY	1 cut(s)	1782
BsrI	ACTGG	4 cut(s)	1248, 1267, 1566, 2150
BssAI	RCCGGY	1 cut(s)	1782
BssECI	CCNNGG	2 cut(s)	825, 1853
BssMI	GATC	9 cut(s)	130, 415, 1076, 1321, 1336, 1719, 1776, 1923, 1984
BssSI	CACGAG	1 cut(s)	2321
BssT1I	CCWWGG	2 cut(s)	825, 1853
Bst2BI	CACGAG	1 cut(s)	2321
Bst2UI	CCWGG	5 cut(s)	251, 801, 1620, 1736, 1934
Bst4CI	ACNGT	4 cut(s)	513, 1012, 1040, 1767
Bst6I	CTCTTC	4 cut(s)	1287, 1473, 1881, 2254
BstAFI	CTTAAG	1 cut(s)	896
BstBI	TTCGAA	1 cut(s)	661
BstDEI	CTNAG	9 cut(s)	75, 213, 735, 855, 997, 1068, 1473, 1535, 2161
BstENI	CCTNNNNNAGG	1 cut(s)	1931
BstF5I	GGATG	9 cut(s)	259, 367, 376, 658, 1039, 1489, 1956, 2066, 2317
BstFNI	CGCG	1 cut(s)	2103
BstHHI	GCGC	2 cut(s)	2103, 2331
BstKTI	GATC	9 cut(s)	133, 418, 1079, 1324, 1339, 1722, 1779, 1926, 1987
BstMAI	GTCTC	6 cut(s)	174, 1500, 1505, 1511, 1700, 1784
BstMBI	GATC	9 cut(s)	130, 415, 1076, 1321, 1336, 1719, 1776, 1923, 1984
BstMWI	GCNNNNNNNGC	4 cut(s)	542, 765, 830, 1972
BstNI	CCWGG	5 cut(s)	251, 801, 1620, 1736, 1934
BstNSI	RCATGY	1 cut(s)	1862
BstSCI	CCNGG	5 cut(s)	249, 799, 1618, 1734, 1932
BstSFI	CTRYAG	1 cut(s)	1085
BstSLI	GKGCMC	1 cut(s)	1224
BstUI	CGCG	1 cut(s)	2103
BstV1I	GCAGC	4 cut(s)	201, 685, 833, 925
BstV2I	GAAGAC	1 cut(s)	1269
BstX2I	RGATCY	3 cut(s)	130, 1923, 1984
BstYI	RGATCY	3 cut(s)	130, 1923, 1984
Bsu36I	CCTNAGG	1 cut(s)	855
BsuRI	GGCC	5 cut(s)	330, 593, 759, 1334, 1868
BtsCI	GGATG	9 cut(s)	259, 367, 376, 658, 1039, 1489, 1956, 2066, 2317
BtsI	GCAGTG	1 cut(s)	631
BtsIMutI	CAGTG	7 cut(s)	120, 631, 807, 1045, 1065, 1573, 2157
BveI	ACCTGC	1 cut(s)	2106
CaiI	CAGNNNCTG	2 cut(s)	643, 2156
CciI	TCATGA	3 cut(s)	82, 982, 1144
CfoI	GCGC	2 cut(s)	2103, 2331
Cfr10I	RCCGGY	1 cut(s)	1782
Cfr13I	GGNCC	5 cut(s)	328, 592, 757, 1090, 1246
CseI	GACGC	2 cut(s)	68, 1419
Csp6I	GTAC	1 cut(s)	2107
CviQI	GTAC	1 cut(s)	2107
DdeI	CTNAG	9 cut(s)	75, 213, 735, 855, 997, 1068, 1473, 1535, 2161
DpnI	GATC	9 cut(s)	132, 417, 1078, 1323, 1338, 1721, 1778, 1925, 1986
DpnII	GATC	9 cut(s)	130, 415, 1076, 1321, 1336, 1719, 1776, 1923, 1984
DraIII	CACNNNGTG	1 cut(s)	1071
Eam1104I	CTCTTC	4 cut(s)	1287, 1473, 1881, 2254
EarI	CTCTTC	4 cut(s)	1287, 1473, 1881, 2254
Ecl136II	GAGCTC	1 cut(s)	2187
Eco130I	CCWWGG	2 cut(s)	825, 1853
Eco24I	GRGCYC	1 cut(s)	2189
Eco31I	GGTCTC	1 cut(s)	1700
Eco32I	GATATC	1 cut(s)	40
Eco47I	GGWCC	2 cut(s)	1090, 1246
Eco53kI	GAGCTC	1 cut(s)	2187
Eco57I	CTGAAG	4 cut(s)	1397, 1425, 1622, 1905
Eco81I	CCTNAGG	1 cut(s)	855
EcoICRI	GAGCTC	1 cut(s)	2187
EcoNI	CCTNNNNNAGG	1 cut(s)	1931
EcoO109I	RGGNCCY	2 cut(s)	757, 1090
EcoRI	GAATTC	1 cut(s)	1137
EcoRII	CCWGG	5 cut(s)	249, 799, 1618, 1734, 1932
EcoRV	GATATC	1 cut(s)	40
EcoT14I	CCWWGG	2 cut(s)	825, 1853
EcoT38I	GRGCYC	1 cut(s)	2189
ErhI	CCWWGG	2 cut(s)	825, 1853
Esp3I	CGTCTC	1 cut(s)	1500
FalI	AAGNNNNNCTT	2 cut(s)	1302, 1334
FaqI	GGGAC	1 cut(s)	2285
FauI	CCCGC	2 cut(s)	1579, 2051
FauNDI	CATATG	1 cut(s)	2118
FbaI	TGATCA	2 cut(s)	415, 1719
Fnu4HI	GCNGC	6 cut(s)	190, 699, 822, 926, 939, 1976
FokI	GGATG	9 cut(s)	266, 374, 383, 665, 1046, 1496, 1943, 2053, 2324
FriOI	GRGCYC	1 cut(s)	2189
Fsp4HI	GCNGC	6 cut(s)	190, 699, 822, 926, 939, 1976
FspBI	CTAG	4 cut(s)	6, 935, 2190, 2211
GlaI	GCGC	2 cut(s)	2102, 2330
GluI	GCNGC	6 cut(s)	190, 699, 822, 926, 939, 1976
GsaI	CCCAGC	1 cut(s)	1229
GsuI	CTGGAG	1 cut(s)	783
HaeIII	GGCC	5 cut(s)	330, 593, 759, 1334, 1868
HapII	CCGG	2 cut(s)	1684, 1783
HgaI	GACGC	2 cut(s)	68, 1419
HhaI	GCGC	2 cut(s)	2103, 2331
Hin6I	GCGC	2 cut(s)	2101, 2329
HinP1I	GCGC	2 cut(s)	2101, 2329
HincII	GTYRAC	3 cut(s)	841, 967, 1272
HindII	GTYRAC	3 cut(s)	841, 967, 1272
HpaII	CCGG	2 cut(s)	1684, 1783
HphI	GGTGA	4 cut(s)	59, 1406, 1451, 2080
Hpy166II	GTNNAC	9 cut(s)	106, 120, 287, 571, 685, 841, 967, 1272, 1732
Hpy8I	GTNNAC	9 cut(s)	106, 120, 287, 571, 685, 841, 967, 1272, 1732
Hpy99I	CGWCG	3 cut(s)	60, 1454, 1457
HpyAV	CCTTC	9 cut(s)	3, 155, 417, 452, 515, 668, 747, 1409, 1859
HpyCH4III	ACNGT	4 cut(s)	513, 1012, 1040, 1767
HpyCH4IV	ACGT	1 cut(s)	289
HpyF10VI	GCNNNNNNNGC	4 cut(s)	542, 765, 830, 1972
HpyF3I	CTNAG	9 cut(s)	75, 213, 735, 855, 997, 1068, 1473, 1535, 2161
HpySE526I	ACGT	1 cut(s)	289
HspAI	GCGC	2 cut(s)	2101, 2329
Ksp22I	TGATCA	2 cut(s)	415, 1719
Kzo9I	GATC	9 cut(s)	130, 415, 1076, 1321, 1336, 1719, 1776, 1923, 1984
LguI	GCTCTTC	1 cut(s)	2254
LmnI	GCTCC	3 cut(s)	1489, 1602, 2184
Lsp1109I	GCAGC	4 cut(s)	201, 685, 833, 925
LweI	GCATC	9 cut(s)	349, 643, 803, 1125, 1485, 1834, 1957, 1965, 2094
MaeI	CTAG	4 cut(s)	6, 935, 2190, 2211
MaeII	ACGT	1 cut(s)	289
MaeIII	GTNAC	5 cut(s)	245, 1063, 1312, 1439, 1800
MalI	GATC	9 cut(s)	132, 417, 1078, 1323, 1338, 1721, 1778, 1925, 1986
MbiI	CCGCTC	1 cut(s)	1975
MboI	GATC	9 cut(s)	130, 415, 1076, 1321, 1336, 1719, 1776, 1923, 1984
MflI	RGATCY	3 cut(s)	130, 1923, 1984
MhlI	GDGCHC	2 cut(s)	1224, 2189
MlyI	GAGTC	4 cut(s)	32, 2121, 2153, 2228
MmeI	TCCRAC	1 cut(s)	999
MroXI	GAANNNNTTC	1 cut(s)	2212
MseI	TTAA	8 cut(s)	585, 672, 897, 909, 945, 1608, 2055, 2344
MslI	CAYNNNNRTG	2 cut(s)	276, 812
MspA1I	CMGCKG	1 cut(s)	1229
MspCI	CTTAAG	1 cut(s)	896
MspI	CCGG	2 cut(s)	1684, 1783
MspR9I	CCNGG	5 cut(s)	251, 801, 1620, 1736, 1934
MvaI	CCWGG	5 cut(s)	251, 801, 1620, 1736, 1934
MvnI	CGCG	1 cut(s)	2103
MwoI	GCNNNNNNNGC	4 cut(s)	542, 765, 830, 1972
NdeI	CATATG	1 cut(s)	2118
NdeII	GATC	9 cut(s)	130, 415, 1076, 1321, 1336, 1719, 1776, 1923, 1984
NlaIV	GGNNCC	4 cut(s)	132, 329, 758, 1223
NmeAIII	GCCGAG	2 cut(s)	356, 1815
NmuCI	GTSAC	2 cut(s)	1063, 1439
NspI	RCATGY	1 cut(s)	1862
NspV	TTCGAA	1 cut(s)	661
OliI	CACNNNNGTG	1 cut(s)	812
PagI	TCATGA	3 cut(s)	82, 982, 1144
PciSI	GCTCTTC	1 cut(s)	2254
PcsI	WCGNNNNNNNCGW	1 cut(s)	1449
PdmI	GAANNNNTTC	1 cut(s)	2212
PflMI	CCANNNNNTGG	1 cut(s)	976
PfoI	TCCNGGA	1 cut(s)	1932
PkrI	GCNGC	6 cut(s)	191, 700, 823, 927, 940, 1977
PleI	GAGTC	4 cut(s)	31, 2121, 2153, 2227
PpsI	GAGTC	4 cut(s)	31, 2121, 2153, 2227
PpuMI	RGGWCCY	1 cut(s)	1090
PshBI	ATTAAT	2 cut(s)	672, 2055
Psp124BI	GAGCTC	1 cut(s)	2189
Psp5II	RGGWCCY	1 cut(s)	1090
Psp6I	CCWGG	5 cut(s)	249, 799, 1618, 1734, 1932
PspFI	CCCAGC	1 cut(s)	1225
PspGI	CCWGG	5 cut(s)	249, 799, 1618, 1734, 1932
PspN4I	GGNNCC	4 cut(s)	132, 329, 758, 1223
PspPI	GGNCC	5 cut(s)	328, 592, 757, 1090, 1246
PspPPI	RGGWCCY	1 cut(s)	1090
PstI	CTGCAG	1 cut(s)	1089
PstNI	CAGNNNCTG	2 cut(s)	643, 2156
PsuI	RGATCY	3 cut(s)	130, 1923, 1984
PvuII	CAGCTG	1 cut(s)	1229
RsaI	GTAC	1 cut(s)	2108
RsaNI	GTAC	1 cut(s)	2107
RseI	CAYNNNNRTG	2 cut(s)	276, 812
SacI	GAGCTC	1 cut(s)	2189
SapI	GCTCTTC	1 cut(s)	2254
SaqAI	TTAA	8 cut(s)	585, 672, 897, 909, 945, 1608, 2055, 2344
SatI	GCNGC	6 cut(s)	190, 699, 822, 926, 939, 1976
Sau3AI	GATC	9 cut(s)	130, 415, 1076, 1321, 1336, 1719, 1776, 1923, 1984
Sau96I	GGNCC	5 cut(s)	328, 592, 757, 1090, 1246
ScaI	AGTACT	1 cut(s)	2108
SchI	GAGTC	4 cut(s)	32, 2121, 2153, 2228
ScrFI	CCNGG	5 cut(s)	251, 801, 1620, 1736, 1934
SduI	GDGCHC	2 cut(s)	1224, 2189
SfaNI	GCATC	9 cut(s)	349, 643, 803, 1125, 1485, 1834, 1957, 1965, 2094
SfcI	CTRYAG	1 cut(s)	1085
SfuI	TTCGAA	1 cut(s)	661
SinI	GGWCC	2 cut(s)	1090, 1246
SmiMI	CAYNNNNRTG	2 cut(s)	276, 812
SmlI	CTYRAG	5 cut(s)	504, 896, 1349, 1613, 1787
SmoI	CTYRAG	5 cut(s)	504, 896, 1349, 1613, 1787
SpeI	ACTAGT	1 cut(s)	2210
SsiI	CCGC	6 cut(s)	913, 926, 1363, 1586, 1975, 2044
SspMI	CTAG	4 cut(s)	6, 935, 2190, 2211
SstI	GAGCTC	1 cut(s)	2189
StyD4I	CCNGG	5 cut(s)	249, 799, 1618, 1734, 1932
StyI	CCWWGG	2 cut(s)	825, 1853
TaaI	ACNGT	4 cut(s)	513, 1012, 1040, 1767
TaiI	ACGT	1 cut(s)	292
TaqI	TCGA	1 cut(s)	661
TatI	WGTACW	1 cut(s)	2106
TauI	GCSGC	2 cut(s)	928, 1978
Tru1I	TTAA	8 cut(s)	585, 672, 897, 909, 945, 1608, 2055, 2344
Tru9I	TTAA	8 cut(s)	585, 672, 897, 909, 945, 1608, 2055, 2344
TscAI	CASTG	7 cut(s)	127, 631, 814, 1045, 1072, 1573, 2157
TseFI	GTSAC	2 cut(s)	1063, 1439
TseI	GCWGC	4 cut(s)	189, 698, 821, 938
Tsp45I	GTSAC	2 cut(s)	1063, 1439
TspGWI	ACGGA	5 cut(s)	406, 1046, 1216, 1819, 1949
TspRI	CASTG	7 cut(s)	127, 631, 814, 1045, 1072, 1573, 2157
Van91I	CCANNNNNTGG	1 cut(s)	976
Vha464I	CTTAAG	1 cut(s)	896
VpaK11BI	GGWCC	2 cut(s)	1090, 1246
VspI	ATTAAT	2 cut(s)	672, 2055
XagI	CCTNNNNNAGG	1 cut(s)	1931
XapI	RAATTY	6 cut(s)	493, 726, 947, 1137, 1281, 1741
XceI	RCATGY	1 cut(s)	1862
XmnI	GAANNNNTTC	1 cut(s)	2212
XspI	CTAG	4 cut(s)	6, 935, 2190, 2211
ZrmI	AGTACT	1 cut(s)	2108

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.