Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0002528

Physical Location & Seq

Genomic Coordinates Reverse (-)

6465807 .. 6470757

Gene Structure 4951 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00004680.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2223 bp

ATGGCTAGACAGCACAGTAGTGAATCGAGTGATGATGATGTGAGGGGTGGAAGAGATAAGTACCGTCAGCATAGAAGTAAAAGCGAGTCGAGGTCAAACAATCATCTTGAGCAGGGTGAGATTGATTCCTCAGATTCTGATAGAGATAATAGAATAAAGAGACAAGGGAGACGGGATGATGAGAAGGAAAGGGAAAGGTCCAGTGAGAGGGTTCCCAGTGATAGACATGGAAGGTGGCGGGGTGATCGTCAAGATAGGGATCGACACACCTCTGATGAAGACGGTGAGGTGGCAGAGAAAGACAGGGATGGGCAAGAAAGGGAGAGGAAAAATGAAAATGGGAACAGACAACGTGAAAGGGATCCCAGTGATAGATATGGAAGGTGGCGGGGTGATCGTCAAGATAGGGATGTACAAACTTCTGATGAAGATGGTGAGCTGGCCGAGAAAGACAGGGGTGGGCAAGAAAGGGAAAGGAAAAATGAAAATGGGAACAGACAACGTGAAAATGGGAATGGTGATAGGAGGAGAGAAAATAGAAGTTCTGAAGATGATGAGCGACTGAAGAGATACCGTCATGAGAGTGGACATAGAAGGCGGGAGGACAAGGATGGTCGTAGAAACAGGGACAGGCATTCACGTCACTACTCTGAGCGCAGGGATGAAAGGGAAGAGCGCAGAACTTCAGAACGGAAAAGGGATAGTAGAGATGATGGCAGGCATAGAAACCGGGATGACGATGGTGAGATAAAGTATCGCAGAAATAAGGAGGTGAATGAAAGAAGAGAAGACACTGAGCGAGATGATAGGATGCTTGAGAATTCAAAACCTCAAGGACGGTCAACATTGCAAGAGGGAAATCTGAATAATAATGTGGCAAATTTGGGAAAGAGTGGTGGGGTTTATATTCCGCCCTATAAGTTGGCCCGAATGATGAAAGAGGTCGAAGATAAAAGTAGCGTCGAGTATCAACGCTTGACATGGGATGCCCTTCGAAAGAGTATCAATGGGCTAGTGAACAAGGTCAATGCTGCAAACATTAAGAATATAATTCCAGAAATTTTTGCAGAGAATCTCATTCGTGGAAGAGGTCTCTTTTGTCGATCCTGTATGAAGTCACAGATGGCATCTCCAGGATTTACAGACGTCTTTGCTGGCTTGGTTGCTGTTGTTAACACCAAGTTTCCTGAGGTTGGTGAACTTCTGTTGAGAAGAATCGTCTTGCAGCTTAAGAGAGCATTTAAGCGGAACGACAAGCCACAACTACTAGCTGCTGTCAAATTTATCGCGCATCTCGTCAACCAACAAGTGGCTCATGAGATTATAGCATTGGAATTGCTTACTGTGCTGCTGGAGAACCCTACCGATGACAGTGTTGAAGTTGCTGTTGGCTTTGTCACAGAGTGTGGATCGATACTGCAAGACCTTTCACCTAAAGGGCTCCATGGCATCTTTGAGCGCTTCCGTGGAATTCTTCATGAAGGAGAAATTGACAAACGAGTTCAGTTTTTGATCGAAGGCTTATTTGCATTACGAAAAGCAAAATTTCAGGGGCACCCAGCTGTTCGTCCGGAACTGGACCTTGTTGAGCAAGAAGACCAGTTAACTCATGAGATTTCTCTTCAAGAGGAGATAAATCCAGAAATTACCCTTGATATTTTCAAGCCTGATCCTGATTTCATTGAGAATGAGAAGCGCTATGAAGATGTGAAGAAAACAATTCTTGGTGAAGACTCTGAAGAGGAAGGTTCTGATGCAGCTTCAGATGGTGAGGATGATGATGATGAGGAGGAGGACGAAGATGATGATGATGAGGAGGACGAGCAGGAAATGAAAATAAGAGATGAGACGGAGACAAATCTTGTGAATCTTCGAAGGACAATATATCTGACTATCATGTCCAGTGTAGATTTTGAGGAAGCAGGTCATAAGCTCCTTAAGATTAAGCTTGAGCCAGGCCAAGAGATGGAGCTATGTGTCATGCTTTTGGAATGTTGCAGTCAGGAGAGAACCTACCTCCGCTATTATGGTCTCTTGGGACAGCGGTTCTGCATGATCAACAGAATCCACCAGGAAAATTTTGAGAAATGTTTTGTCCAGCAATATTCAATGATTCACCGGCTTGAAACAAACAAGCTACGTAATGTAGCTAAGTTTTTGCGCGCATTTACTCGGAACAGATGCCCTTCCGTGGCATTGCTTAGCCTATATACGGTTAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0071006 GO:0071012 GO:0071013 GO:0071704 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MA3 MIF4G

Protein Analysis

740

Amino Acids

86.84

Weight (kDa)

5.57

Isoelectric Point (pI)

58.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MIF4G	PF02854	333 - 514	9.2e-14	MIF4G domain
MA3	PF02847	624 - 722	3e-27	MA3 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002528)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G80930
fragaria_vesca	FvH4_3g01450 FvH4_3g43230 FvH4_3g43240 FvH4_3g43240
malus_domestica	MD03G1023500.v1.1 MD11G1028600.v1.1
prunus_persica	Prupe.6G022700_v2.0.a1 Prupe.6G022700_v2.0.a1
pyrus_communis	pycom03g02010 pycom11g02210
rosa_chinensis	RchiOBHm_Chr5g0002291 RchiOBHm_Chr5g0077281 RchiOBHm_Chr5g0077291
rosa_laevigata	RLG00000031014 RLG00000036681 RLG00000036682
rosa_multiflora	Rmu_co8446135.1_g000001 Rmu_sc0000433.1_g000042 Rmu_sc0001492.1_g000008 Rmu_sc0005576.1_g000001 Rmu_sc0006137.1_g000009 Rmu_sc0008383.1_g000001 Rmu_sc0013155.1_g000012 Rmu_ssc0000410.1_g000040
rosa_roxburghii	Rroxscaffold_1G00004680 Rroxscaffold_1G00073650
rosa_rugosa	Rorug04G0394900 Rorug05G0453500
rosa_samantha	Rh5AG017700 Rh5AG508400 Rh5AG508500 Rh5BG020300 Rh5BG529600 Rh5CG019100 Rh5CG019400 Rh5CG554100 Rh5DG018300 Rh5DG542200
rosa_wichuraiana	Rw5G046900 Rw5G046910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1897
AatII	GACGTC	1 cut(s)	1149
Acc36I	ACCTGC	1 cut(s)	1913
AccB1I	GGYRCC	1 cut(s)	1554
AccB7I	CCANNNNNTGG	2 cut(s)	1309, 1966
AccII	CGCG	2 cut(s)	1289, 2163
AccIII	TCCGGA	1 cut(s)	1570
AciI	CCGC	7 cut(s)	238, 388, 598, 911, 1246, 2020, 2044
AclWI	GGATC	6 cut(s)	267, 356, 369, 1098, 1417, 1664
AcoI	YGGCCR	1 cut(s)	441
AcsI	RAATTY	7 cut(s)	820, 880, 1059, 1280, 1470, 1544, 2077
AcuI	CTGAAG	5 cut(s)	567, 584, 669, 1746, 1758
AcyI	GRCGYC	1 cut(s)	1146
AdeI	CACNNNGTG	1 cut(s)	1404
AfaI	GTAC	2 cut(s)	62, 414
AfeI	AGCGCT	2 cut(s)	1460, 1697
AfiI	CCNNNNNNNGG	6 cut(s)	207, 1193, 1309, 1966, 2191, 2212
AflII	CTTAAG	2 cut(s)	1229, 1937
AgsI	TTSAA	6 cut(s)	825, 1379, 1625, 1663, 2109, 2126
AjiI	CACGTC	1 cut(s)	641
AjnI	CCWGG	3 cut(s)	1132, 1954, 2070
AjuI	GAANNNNNNNTTGG	2 cut(s)	1371, 1403
AleI	CACNNNNGTG	1 cut(s)	18
Alw26I	GTCTC	6 cut(s)	154, 163, 1097, 1841, 1847, 2036
AlwI	GGATC	6 cut(s)	267, 356, 369, 1098, 1417, 1664
AlwNI	CAGNNNCTG	1 cut(s)	137
Aor13HI	TCCGGA	1 cut(s)	1570
Aor51HI	AGCGCT	2 cut(s)	1460, 1697
AoxI	GGCC	3 cut(s)	441, 924, 1957
ApeKI	GCWGC	5 cut(s)	1031, 1225, 1271, 1348, 1757
ApoI	RAATTY	7 cut(s)	820, 880, 1059, 1280, 1470, 1544, 2077
AspLEI	GCGC	7 cut(s)	657, 678, 1291, 1461, 1698, 2163, 2165
AspS9I	GGNCC	3 cut(s)	198, 925, 1579
AsuC2I	CCSGG	1 cut(s)	731
AsuII	TTCGAA	2 cut(s)	994, 1873
AvaII	GGWCC	2 cut(s)	198, 1579
AxyI	CCTNAGG	1 cut(s)	1188
BaeGI	GKGCMC	1 cut(s)	1557
BamHI	GGATCC	1 cut(s)	361
BanI	GGYRCC	1 cut(s)	1554
BanII	GRGCYC	1 cut(s)	1443
BbsI	GAAGAC	4 cut(s)	285, 795, 1602, 1737
BbvI	GCAGC	5 cut(s)	1018, 1237, 1258, 1335, 1769
BccI	CCATC	8 cut(s)	302, 425, 605, 707, 734, 1117, 1760, 1960
BciT130I	CCWGG	3 cut(s)	1134, 1956, 2072
BclI	TGATCA	1 cut(s)	2055
BcnI	CCSGG	1 cut(s)	731
BcoDI	GTCTC	6 cut(s)	154, 163, 1097, 1841, 1847, 2036
BfaI	CTAG	3 cut(s)	6, 1013, 1268
BfoI	RGCGCY	2 cut(s)	1462, 1699
BfrI	CTTAAG	2 cut(s)	1229, 1937
BfuAI	ACCTGC	1 cut(s)	1913
BisI	GCNGC	5 cut(s)	1032, 1226, 1272, 1349, 1758
BlpI	GCTNAGC	1 cut(s)	2201
BlsI	GCNGC	5 cut(s)	1033, 1227, 1273, 1350, 1759
Bme1390I	CCNGG	4 cut(s)	731, 1134, 1956, 2072
Bme18I	GGWCC	2 cut(s)	198, 1579
BmgBI	CACGTC	1 cut(s)	641
BmgT120I	GGNCC	3 cut(s)	198, 925, 1579
BmiI	GGNNCC	4 cut(s)	213, 363, 1442, 1556
BmrFI	CCNGG	4 cut(s)	731, 1134, 1956, 2072
BmrI	ACTGGG	2 cut(s)	210, 360
BmsI	GCATC	7 cut(s)	801, 976, 1136, 1300, 1458, 1744, 2171
BmuI	ACTGGG	2 cut(s)	210, 360
BpiI	GAAGAC	4 cut(s)	285, 795, 1602, 1737
BpmI	CTGGAG	2 cut(s)	1116, 1373
Bpu1102I	GCTNAGC	1 cut(s)	2201
Bpu14I	TTCGAA	2 cut(s)	994, 1873
BpuEI	CTTGAG	4 cut(s)	128, 816, 836, 1970
BpuMI	CCSGG	1 cut(s)	731
Bsa29I	ATCGAT	1 cut(s)	1412
BsaAI	YACGTR	1 cut(s)	2141
BsaBI	GATNNNNATC	1 cut(s)	258
BsaHI	GRCGYC	1 cut(s)	1146
BsaI	GGTCTC	2 cut(s)	1097, 2036
BsaJI	CCNNGG	3 cut(s)	1444, 1465, 2190
BsaWI	WCCGGW	1 cut(s)	1570
Bsc4I	CCNNNNNNNGG	6 cut(s)	207, 1193, 1309, 1966, 2191, 2212
Bse118I	RCCGGY	1 cut(s)	2118
Bse1I	ACTGG	6 cut(s)	201, 216, 366, 1581, 1600, 1902
Bse21I	CCTNAGG	1 cut(s)	1188
Bse3DI	GCAATG	2 cut(s)	845, 2195
Bse8I	GATNNNNATC	1 cut(s)	258
BseAI	TCCGGA	1 cut(s)	1570
BseBI	CCWGG	3 cut(s)	1134, 1956, 2072
BseCI	ATCGAT	1 cut(s)	1412
BseDI	CCNNGG	3 cut(s)	1444, 1465, 2190
BseGI	GGATG	9 cut(s)	181, 313, 415, 616, 667, 739, 816, 991, 1780
BseJI	GATNNNNATC	1 cut(s)	258
BseLI	CCNNNNNNNGG	6 cut(s)	207, 1193, 1309, 1966, 2191, 2212
BseMI	GCAATG	2 cut(s)	845, 2195
BseMII	CTCAG	4 cut(s)	144, 642, 786, 1179
BseNI	ACTGG	6 cut(s)	201, 216, 366, 1581, 1600, 1902
BsePI	GCGCGC	1 cut(s)	2161
BseRI	GAGGAG	5 cut(s)	541, 1643, 1802, 1805, 1829
BseSI	GKGCMC	1 cut(s)	1557
BseXI	GCAGC	5 cut(s)	1018, 1237, 1258, 1335, 1769
BseYI	CCCAGC	1 cut(s)	1558
Bsh1236I	CGCG	2 cut(s)	1289, 2163
BshFI	GGCC	3 cut(s)	443, 926, 1959
BshNI	GGYRCC	1 cut(s)	1554
BshVI	ATCGAT	1 cut(s)	1412
BsiSI	CCGG	3 cut(s)	730, 1571, 2119
BslFI	GGGAC	2 cut(s)	641, 2052
BslI	CCNNNNNNNGG	6 cut(s)	207, 1193, 1309, 1966, 2191, 2212
BsmAI	GTCTC	6 cut(s)	154, 163, 1097, 1841, 1847, 2036
BsmBI	CGTCTC	2 cut(s)	163, 1841
BsmFI	GGGAC	2 cut(s)	641, 2052
BsmI	GAATGC	1 cut(s)	634
BsnI	GGCC	3 cut(s)	443, 926, 1959
Bso31I	GGTCTC	2 cut(s)	1097, 2036
Bsp119I	TTCGAA	2 cut(s)	994, 1873
Bsp1286I	GDGCHC	2 cut(s)	1443, 1557
Bsp13I	TCCGGA	1 cut(s)	1570
Bsp1407I	TGTACA	1 cut(s)	412
Bsp143I	GATC	9 cut(s)	244, 259, 361, 394, 1103, 1409, 1512, 1669, 2055
Bsp1720I	GCTNAGC	1 cut(s)	2201
Bsp19I	CCATGG	1 cut(s)	1444
BspACI	CCGC	7 cut(s)	238, 388, 598, 911, 1246, 2020, 2044
BspANI	GGCC	3 cut(s)	443, 926, 1959
BspCNI	CTCAG	4 cut(s)	143, 643, 787, 1180
BspDI	ATCGAT	1 cut(s)	1412
BspEI	TCCGGA	1 cut(s)	1570
BspFNI	CGCG	2 cut(s)	1289, 2163
BspHI	TCATGA	4 cut(s)	577, 1315, 1477, 1609
BspLI	GGNNCC	4 cut(s)	213, 363, 1442, 1556
BspMI	ACCTGC	1 cut(s)	1913
BspPI	GGATC	6 cut(s)	267, 356, 369, 1098, 1417, 1664
BspQI	GCTCTTC	1 cut(s)	666
BspT104I	TTCGAA	2 cut(s)	994, 1873
BspT107I	GGYRCC	1 cut(s)	1554
BspTI	CTTAAG	2 cut(s)	1229, 1937
BspTNI	GGTCTC	2 cut(s)	1097, 2036
BsrDI	GCAATG	2 cut(s)	845, 2195
BsrFI	RCCGGY	1 cut(s)	2118
BsrGI	TGTACA	1 cut(s)	412
BsrI	ACTGG	6 cut(s)	201, 216, 366, 1581, 1600, 1902
BssAI	RCCGGY	1 cut(s)	2118
BssECI	CCNNGG	3 cut(s)	1444, 1465, 2190
BssHII	GCGCGC	1 cut(s)	2161
BssMI	GATC	9 cut(s)	244, 259, 361, 394, 1103, 1409, 1512, 1669, 2055
BssNI	GRCGYC	1 cut(s)	1146
BssT1I	CCWWGG	1 cut(s)	1444
Bst2UI	CCWGG	3 cut(s)	1134, 1956, 2072
Bst4CI	ACNGT	8 cut(s)	17, 65, 284, 575, 840, 1345, 1373, 2215
Bst6I	CTCTTC	7 cut(s)	46, 560, 666, 778, 1081, 1626, 1734
BstACI	GRCGYC	1 cut(s)	1146
BstAFI	CTTAAG	2 cut(s)	1229, 1937
BstAUI	TGTACA	1 cut(s)	412
BstBAI	YACGTR	1 cut(s)	2141
BstBI	TTCGAA	2 cut(s)	994, 1873
BstC8I	GCNNGC	4 cut(s)	441, 719, 1156, 2163
BstDEI	CTNAG	6 cut(s)	130, 651, 795, 1188, 2151, 2201
BstDSI	CCRYGG	3 cut(s)	1444, 1465, 2190
BstF5I	GGATG	9 cut(s)	181, 313, 415, 616, 667, 739, 816, 991, 1780
BstFNI	CGCG	2 cut(s)	1289, 2163
BstH2I	RGCGCY	2 cut(s)	1462, 1699
BstHHI	GCGC	7 cut(s)	657, 678, 1291, 1461, 1698, 2163, 2165
BstKTI	GATC	9 cut(s)	247, 262, 364, 397, 1106, 1412, 1515, 1672, 2058
BstMAI	GTCTC	6 cut(s)	154, 163, 1097, 1841, 1847, 2036
BstMBI	GATC	9 cut(s)	244, 259, 361, 394, 1103, 1409, 1512, 1669, 2055
BstMWI	GCNNNNNNNGC	1 cut(s)	1345
BstNI	CCWGG	3 cut(s)	1134, 1956, 2072
BstSCI	CCNGG	4 cut(s)	729, 1132, 1954, 2070
BstSLI	GKGCMC	1 cut(s)	1557
BstSNI	TACGTA	1 cut(s)	2141
BstUI	CGCG	2 cut(s)	1289, 2163
BstV1I	GCAGC	5 cut(s)	1018, 1237, 1258, 1335, 1769
BstV2I	GAAGAC	4 cut(s)	285, 795, 1602, 1737
BstX2I	RGATCY	1 cut(s)	361
BstYI	RGATCY	1 cut(s)	361
Bsu15I	ATCGAT	1 cut(s)	1412
Bsu36I	CCTNAGG	1 cut(s)	1188
BsuRI	GGCC	3 cut(s)	443, 926, 1959
BsuTUI	ATCGAT	1 cut(s)	1412
BtgI	CCRYGG	3 cut(s)	1444, 1465, 2190
BtrI	CACGTC	1 cut(s)	641
BtsCI	GGATG	9 cut(s)	181, 313, 415, 616, 667, 739, 816, 991, 1780
BtsIMutI	CAGTG	6 cut(s)	208, 223, 373, 792, 1378, 1909
BveI	ACCTGC	1 cut(s)	1913
Cac8I	GCNNGC	4 cut(s)	441, 719, 1156, 2163
CaiI	CAGNNNCTG	1 cut(s)	137
CciI	TCATGA	4 cut(s)	577, 1315, 1477, 1609
CfoI	GCGC	7 cut(s)	657, 678, 1291, 1461, 1698, 2163, 2165
Cfr10I	RCCGGY	1 cut(s)	2118
Cfr13I	GGNCC	3 cut(s)	198, 925, 1579
ClaI	ATCGAT	1 cut(s)	1412
CseI	GACGC	1 cut(s)	949
Csp6I	GTAC	2 cut(s)	61, 413
CviQI	GTAC	2 cut(s)	61, 413
DdeI	CTNAG	6 cut(s)	130, 651, 795, 1188, 2151, 2201
DpnI	GATC	9 cut(s)	246, 261, 363, 396, 1105, 1411, 1514, 1671, 2057
DpnII	GATC	9 cut(s)	244, 259, 361, 394, 1103, 1409, 1512, 1669, 2055
DraIII	CACNNNGTG	1 cut(s)	1404
DrdI	GACNNNNNNGTC	1 cut(s)	1897
DseDI	GACNNNNNNGTC	1 cut(s)	1897
EaeI	YGGCCR	1 cut(s)	441
Eam1104I	CTCTTC	7 cut(s)	46, 560, 666, 778, 1081, 1626, 1734
EarI	CTCTTC	7 cut(s)	46, 560, 666, 778, 1081, 1626, 1734
EciI	GGCGGA	1 cut(s)	900
Eco105I	TACGTA	1 cut(s)	2141
Eco130I	CCWWGG	1 cut(s)	1444
Eco24I	GRGCYC	1 cut(s)	1443
Eco31I	GGTCTC	2 cut(s)	1097, 2036
Eco47I	GGWCC	2 cut(s)	198, 1579
Eco47III	AGCGCT	2 cut(s)	1460, 1697
Eco57I	CTGAAG	5 cut(s)	567, 584, 669, 1746, 1758
Eco81I	CCTNAGG	1 cut(s)	1188
EcoRI	GAATTC	2 cut(s)	820, 1470
EcoRII	CCWGG	3 cut(s)	1132, 1954, 2070
EcoT14I	CCWWGG	1 cut(s)	1444
EcoT38I	GRGCYC	1 cut(s)	1443
ErhI	CCWWGG	1 cut(s)	1444
Esp3I	CGTCTC	2 cut(s)	163, 1841
FaqI	GGGAC	2 cut(s)	641, 2052
FauI	CCCGC	3 cut(s)	231, 381, 591
FbaI	TGATCA	1 cut(s)	2055
Fnu4HI	GCNGC	5 cut(s)	1032, 1226, 1272, 1349, 1758
FokI	GGATG	9 cut(s)	188, 320, 422, 623, 674, 746, 823, 998, 1787
FriOI	GRGCYC	1 cut(s)	1443
Fsp4HI	GCNGC	5 cut(s)	1032, 1226, 1272, 1349, 1758
FspBI	CTAG	3 cut(s)	6, 1013, 1268
GlaI	GCGC	7 cut(s)	656, 677, 1290, 1460, 1697, 2162, 2164
GluI	GCNGC	5 cut(s)	1032, 1226, 1272, 1349, 1758
GsaI	CCCAGC	1 cut(s)	1562
GsuI	CTGGAG	2 cut(s)	1116, 1373
HaeII	RGCGCY	2 cut(s)	1462, 1699
HaeIII	GGCC	3 cut(s)	443, 926, 1959
HapII	CCGG	3 cut(s)	730, 1571, 2119
HgaI	GACGC	1 cut(s)	949
HhaI	GCGC	7 cut(s)	657, 678, 1291, 1461, 1698, 2163, 2165
Hin1I	GRCGYC	1 cut(s)	1146
Hin6I	GCGC	7 cut(s)	655, 676, 1289, 1459, 1696, 2161, 2163
HinP1I	GCGC	7 cut(s)	655, 676, 1289, 1459, 1696, 2161, 2163
HincII	GTYRAC	5 cut(s)	843, 1174, 1300, 1605, 2218
HindII	GTYRAC	5 cut(s)	843, 1174, 1300, 1605, 2218
HindIII	AAGCTT	1 cut(s)	1946
HpaI	GTTAAC	3 cut(s)	1174, 1605, 2218
HpaII	CCGG	3 cut(s)	730, 1571, 2119
Hpy166II	GTNNAC	8 cut(s)	587, 843, 1018, 1174, 1199, 1300, 1605, 2218
Hpy8I	GTNNAC	8 cut(s)	587, 843, 1018, 1174, 1199, 1300, 1605, 2218
Hpy99I	CGWCG	1 cut(s)	965
HpyCH4III	ACNGT	8 cut(s)	17, 65, 284, 575, 840, 1345, 1373, 2215
HpyCH4IV	ACGT	5 cut(s)	352, 502, 640, 1146, 2140
HpyCH4V	TGCA	9 cut(s)	850, 1034, 1067, 1225, 1420, 1529, 1757, 1998, 2052
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1345
HpyF3I	CTNAG	6 cut(s)	130, 651, 795, 1188, 2151, 2201
HpySE526I	ACGT	5 cut(s)	352, 502, 640, 1146, 2140
Hsp92I	GRCGYC	1 cut(s)	1146
HspAI	GCGC	7 cut(s)	655, 676, 1289, 1459, 1696, 2161, 2163
Kpn2I	TCCGGA	1 cut(s)	1570
Ksp22I	TGATCA	1 cut(s)	2055
KspAI	GTTAAC	3 cut(s)	1174, 1605, 2218
Kzo9I	GATC	9 cut(s)	244, 259, 361, 394, 1103, 1409, 1512, 1669, 2055
LguI	GCTCTTC	1 cut(s)	666
LmnI	GCTCC	3 cut(s)	1446, 1938, 1969
Lsp1109I	GCAGC	5 cut(s)	1018, 1237, 1258, 1335, 1769
LweI	GCATC	7 cut(s)	801, 976, 1136, 1300, 1458, 1744, 2171
MaeI	CTAG	3 cut(s)	6, 1013, 1268
MaeII	ACGT	5 cut(s)	352, 502, 640, 1146, 2140
MaeIII	GTNAC	3 cut(s)	641, 1116, 1396
MalI	GATC	9 cut(s)	246, 261, 363, 396, 1105, 1411, 1514, 1671, 2057
MboI	GATC	9 cut(s)	244, 259, 361, 394, 1103, 1409, 1512, 1669, 2055
MflI	RGATCY	1 cut(s)	361
MhlI	GDGCHC	2 cut(s)	1443, 1557
MlyI	GAGTC	2 cut(s)	95, 1727
MroI	TCCGGA	1 cut(s)	1570
MseI	TTAA	8 cut(s)	1041, 1173, 1230, 1242, 1604, 1938, 1944, 2217
MslI	CAYNNNNRTG	2 cut(s)	18, 582
MspA1I	CMGCKG	2 cut(s)	1562, 2044
MspCI	CTTAAG	2 cut(s)	1229, 1937
MspI	CCGG	3 cut(s)	730, 1571, 2119
MspR9I	CCNGG	4 cut(s)	731, 1134, 1956, 2072
Mva1269I	GAATGC	1 cut(s)	634
MvaI	CCWGG	3 cut(s)	1134, 1956, 2072
MvnI	CGCG	2 cut(s)	1289, 2163
MwoI	GCNNNNNNNGC	1 cut(s)	1345
NciI	CCSGG	1 cut(s)	731
NcoI	CCATGG	1 cut(s)	1444
NdeII	GATC	9 cut(s)	244, 259, 361, 394, 1103, 1409, 1512, 1669, 2055
NlaIV	GGNNCC	4 cut(s)	213, 363, 1442, 1556
NmeAIII	GCCGAG	1 cut(s)	469
NmuCI	GTSAC	3 cut(s)	641, 1116, 1396
NspV	TTCGAA	2 cut(s)	994, 1873
OliI	CACNNNNGTG	1 cut(s)	18
PagI	TCATGA	4 cut(s)	577, 1315, 1477, 1609
PauI	GCGCGC	1 cut(s)	2161
PciSI	GCTCTTC	1 cut(s)	666
PcsI	WCGNNNNNNNCGW	1 cut(s)	1293
PctI	GAATGC	1 cut(s)	634
PfeI	GAWTC	8 cut(s)	23, 125, 134, 1072, 1215, 1867, 2064, 2113
PflMI	CCANNNNNTGG	2 cut(s)	1309, 1966
PfoI	TCCNGGA	1 cut(s)	1132
PkrI	GCNGC	5 cut(s)	1033, 1227, 1273, 1350, 1759
PleI	GAGTC	2 cut(s)	94, 1727
PpsI	GAGTC	2 cut(s)	94, 1727
Ppu21I	YACGTR	1 cut(s)	2141
Psp6I	CCWGG	3 cut(s)	1132, 1954, 2070
PspFI	CCCAGC	1 cut(s)	1558
PspGI	CCWGG	3 cut(s)	1132, 1954, 2070
PspN4I	GGNNCC	4 cut(s)	213, 363, 1442, 1556
PspPI	GGNCC	3 cut(s)	198, 925, 1579
PstNI	CAGNNNCTG	1 cut(s)	137
PsuI	RGATCY	1 cut(s)	361
PteI	GCGCGC	1 cut(s)	2161
PvuII	CAGCTG	1 cut(s)	1562
RsaI	GTAC	2 cut(s)	62, 414
RsaNI	GTAC	2 cut(s)	61, 413
RseI	CAYNNNNRTG	2 cut(s)	18, 582
SapI	GCTCTTC	1 cut(s)	666
SaqAI	TTAA	8 cut(s)	1041, 1173, 1230, 1242, 1604, 1938, 1944, 2217
SatI	GCNGC	5 cut(s)	1032, 1226, 1272, 1349, 1758
Sau3AI	GATC	9 cut(s)	244, 259, 361, 394, 1103, 1409, 1512, 1669, 2055
Sau96I	GGNCC	3 cut(s)	198, 925, 1579
SchI	GAGTC	2 cut(s)	95, 1727
ScrFI	CCNGG	4 cut(s)	731, 1134, 1956, 2072
SduI	GDGCHC	2 cut(s)	1443, 1557
SfaNI	GCATC	7 cut(s)	801, 976, 1136, 1300, 1458, 1744, 2171
SfuI	TTCGAA	2 cut(s)	994, 1873
SinI	GGWCC	2 cut(s)	198, 1579
SmiMI	CAYNNNNRTG	2 cut(s)	18, 582
SmlI	CTYRAG	6 cut(s)	107, 815, 831, 1229, 1937, 1949
SmoI	CTYRAG	6 cut(s)	107, 815, 831, 1229, 1937, 1949
SnaBI	TACGTA	1 cut(s)	2141
SsiI	CCGC	7 cut(s)	238, 388, 598, 911, 1246, 2020, 2044
SspI	AATATT	1 cut(s)	2105
SspMI	CTAG	3 cut(s)	6, 1013, 1268
StyD4I	CCNGG	4 cut(s)	729, 1132, 1954, 2070
StyI	CCWWGG	1 cut(s)	1444
TaaI	ACNGT	8 cut(s)	17, 65, 284, 575, 840, 1345, 1373, 2215
TaiI	ACGT	5 cut(s)	355, 505, 643, 1149, 2143
TatI	WGTACW	1 cut(s)	412
TfiI	GAWTC	8 cut(s)	23, 125, 134, 1072, 1215, 1867, 2064, 2113
Tru1I	TTAA	8 cut(s)	1041, 1173, 1230, 1242, 1604, 1938, 1944, 2217
Tru9I	TTAA	8 cut(s)	1041, 1173, 1230, 1242, 1604, 1938, 1944, 2217
TscAI	CASTG	6 cut(s)	208, 223, 373, 799, 1378, 1909
TseFI	GTSAC	3 cut(s)	641, 1116, 1396
TseI	GCWGC	5 cut(s)	1031, 1225, 1271, 1348, 1757
Tsp45I	GTSAC	3 cut(s)	641, 1116, 1396
TspGWI	ACGGA	4 cut(s)	706, 1454, 1865, 2179
TspRI	CASTG	6 cut(s)	208, 223, 373, 799, 1378, 1909
Van91I	CCANNNNNTGG	2 cut(s)	1309, 1966
Vha464I	CTTAAG	2 cut(s)	1229, 1937
VpaK11BI	GGWCC	2 cut(s)	198, 1579
XapI	RAATTY	7 cut(s)	820, 880, 1059, 1280, 1470, 1544, 2077
XspI	CTAG	3 cut(s)	6, 1013, 1268
ZraI	GACGTC	1 cut(s)	1147

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.