Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0002528

Physical Location & Seq

Genomic Coordinates Reverse (-)

2505502 .. 2510872

Gene Structure 5371 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1028600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2340 bp

ATGGCTAGAAGATATAGTAGCGATTCAAGTGATGATGATGTGAGAGGTGAATATCGTCATCGTAGAAGTAGAACTAAGCGACATGATGAGCGTCATAGAGATGTGGACAGGGAGAAGTCCACTGAGAGGGATCCTAGAGGTAGAGAAAGTTCTGATGAAGAAGGGGAGTTGATAGAGAGAGACAGGGGCAGCCAGAGGAATGAAAGAAAGCCTGAGAATGGGCGTAGAGAACGAGAAAAAGGTAATTACCGGGATGAAAGGGATAAACCACATAGGCGTGAGAATGAACGTAGAGGGGTAGATACTGATGGCGCTCATAGAAGTAGGGGTCGTGATTCACGTCACTATTCAGAGCACAAGGATGAAAGGGAAGAGCACAGAACATTAGAATGGAAAAATGATAAAAGAGATGATGATCAGAGAAGGGGGCATAAAAACCAGGAAGATGAAAGACGAGAAGGAGAAGACAGTAGAACTTTGAGGGAAAAGAATGCAAATTCAATACCTCAAGGACGGTCGCAAGAAGGGAACATGAATGCTGATGTGGCAAAATCAGGAAAGAGTGGTGGAGTTTACATTCCCCCGTTTAAGTTGGCCCGTATGATGAAAGAGGTTGAAGATAAAAGCAGTGTTCAGTATCAGCGACTGACATGGGATGCCCTTCGAAAGAGCATCAATGGTCTTGTGAACAAGGTCAATGCTGCAAACATAAAAAATATAATTCCTGAAATTTTTGCTGAGAATCTGATTCGTGGAAGGGGCCTTTTCTGCCGATCCTGCATGAAATCACAGATGGCATCTCCAGGTTTCACTGATGTGTTTGCAGCCTTGGTTGCTGTTGTCAACACAAAGTTTCCCGAGGTTGGTGAACTTTTGTTGAGAAGAATTGTCTTACAGCTTAAAAGAGCATTCAAGCGGAATGACAAGCCGCAACTACTAGCTGCCGTTAAATTTATAGCGCATCTGGTCAACCAGCAAGTAGCTCATGAGATTATTGCCTTGGAATTACTCACTGTTCTGCTGGAGAACCCTACGGATGACAGTGTTGAAGTTGCTGTTGGCTTTGTCACTGAATGTGGATCATTACTGCAGGACCTTTCGCCTAAAGGGTTGCACGGCATCTTTGAACGCTTTCGTGGAATTCTTCATGAGGGAGAAATAGACAAGAGAGTTCAATATCTGATTGAAAACTTATTTGCAATACGGAAAGCGAAGTTTCAGGGGCACCCAGCTGTTCGTCCAGAACTGGACCTTGTAGAGCAGGAAGACCAGTTGACACATGAAATTTCTCTTGAAGAGGAAATAGATTCAGAAGTTACCCTTGATCTTTTCAAGGCCGATCCTAATTTCCTTGAGAATGAGAAGCGCTATGAAGAACTGAAGAAAGCTATACTTGGTGAGGATTCTGAAGATGAAGAAGGTTCTGATGCAGCTTCAGATGGTGAAGATGACGATGATGACGATGATGAGGAATCTGAGGAAGAGGATGAGGAGCAGATGCAGATAAGAGATGAGACAGAGACAAATCTTGTGAATCTTAGAAGAACTATCTATCTTACAATCATGTCCAGTGTAGATTTTGAGGAGGCAGGTCATAAGCTCCTGAAGATTAAACTTGAGCCTGGTCAAGAGATGGAACTGTGTGTCATGCTTTTGGAATGTTGCAGTCAAGAAAGAACCTACCTCCGATATTATGGTCTTTTGGGCCAAAGGTTCTGCATGATCAACAAAGTCCACCAGGAAAATTTTGAGAAATGCTTTGTGCAACAGTATTCCATGATCCACCGGCTTGAAACAAATAAGCTACGGAATGTTGCAAAGTTTTTCGCCCATTTACTCGGCACAGACGCCCTTCCTTGGCATGTGTTGGCCTATATACGATTGACTGAAGAGGATACTACTTCTTCTTCTCGTATTTTTATCAAGATCCTTTTCCAGGAATTGTCGGAGCATCTTGGTATCCGTTTGCTAAATGAGCGGCTCACAGATCCTGCAATGCAGGAGTCTTATGATTCTATCTTCCCAAAGGATAATCCCAAGAACACCCGCTTTGCCATTAATTTTTTCACATCCATTGGGCTTGGTGGCATCACCGAGAACCTGCGCGAGTACTTGAAGCATATGCCACGACTCATTATGGAACAACAAAAACAAGTGTCTGACTCAGAATCAGATGAAGAAGAATCTGGGAGCTCTGGTTCATCAGATTCAGGATCTAGTTCTGAGTCTGAATCAGAATCATCAAGCTCTTCTGAAAGTGATAGGGACAAAAGACGCAAGAAGCGTAGGAGTGAAAGCAAGAGGGACGAAAGACATGAGAAGCGCAGGAGGAGAGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0071006 GO:0071012 GO:0071013 GO:0071704 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MA3 MIF4G

Protein Analysis

780

Amino Acids

90.59

Weight (kDa)

5.48

Isoelectric Point (pI)

61.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MIF4G	PF02854	223 - 404	1.1e-15	MIF4G domain
MA3	PF02847	513 - 619	1.3e-30	MA3 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002528)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G80930
fragaria_vesca	FvH4_3g01450 FvH4_3g43230 FvH4_3g43240 FvH4_3g43240
malus_domestica	MD03G1023500.v1.1 MD11G1028600.v1.1
prunus_persica	Prupe.6G022700_v2.0.a1 Prupe.6G022700_v2.0.a1
pyrus_communis	pycom03g02010 pycom11g02210
rosa_chinensis	RchiOBHm_Chr5g0002291 RchiOBHm_Chr5g0077281 RchiOBHm_Chr5g0077291
rosa_laevigata	RLG00000031014 RLG00000036681 RLG00000036682
rosa_multiflora	Rmu_co8446135.1_g000001 Rmu_sc0000433.1_g000042 Rmu_sc0001492.1_g000008 Rmu_sc0005576.1_g000001 Rmu_sc0006137.1_g000009 Rmu_sc0008383.1_g000001 Rmu_sc0013155.1_g000012 Rmu_ssc0000410.1_g000040
rosa_roxburghii	Rroxscaffold_1G00004680 Rroxscaffold_1G00073650
rosa_rugosa	Rorug04G0394900 Rorug05G0453500
rosa_samantha	Rh5AG017700 Rh5AG508400 Rh5AG508500 Rh5BG020300 Rh5BG529600 Rh5CG019100 Rh5CG019400 Rh5CG554100 Rh5DG018300 Rh5DG542200
rosa_wichuraiana	Rw5G046900 Rw5G046910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1580, 2109
AccB1I	GGYRCC	1 cut(s)	1224
AccBSI	CCGCTC	1 cut(s)	1978
AccII	CGCG	1 cut(s)	2106
AciI	CCGC	4 cut(s)	916, 929, 1978, 2047
AclWI	GGATC	9 cut(s)	125, 138, 768, 1087, 1334, 1774, 1921, 1982, 2221
AcsI	RAATTY	6 cut(s)	496, 729, 950, 1140, 1284, 1744
AcuI	CTGAAG	5 cut(s)	1400, 1419, 1428, 1625, 1908
AcyI	GRCGYC	1 cut(s)	1848
AfaI	GTAC	1 cut(s)	2111
AfeI	AGCGCT	1 cut(s)	1367
AfiI	CCNNNNNNNGG	5 cut(s)	126, 218, 863, 1857, 1936
AjiI	CACGTC	1 cut(s)	341
AjnI	CCWGG	5 cut(s)	438, 802, 1621, 1737, 1935
AjuI	GAANNNNNNNTTGG	2 cut(s)	1041, 1073
AleI	CACNNNNGTG	1 cut(s)	815
AloI	GAACNNNNNNTCC	2 cut(s)	2182, 2214
Alw21I	GWGCWC	3 cut(s)	357, 378, 2195
Alw26I	GTCTC	3 cut(s)	174, 1508, 1514
AlwI	GGATC	9 cut(s)	125, 138, 768, 1087, 1334, 1774, 1921, 1982, 2221
AlwNI	CAGNNNCTG	2 cut(s)	646, 1991
Ama87I	CYCGRG	1 cut(s)	857
Aor51HI	AGCGCT	1 cut(s)	1367
AoxI	GGCC	5 cut(s)	594, 760, 1335, 1705, 1869
ApeKI	GCWGC	5 cut(s)	189, 701, 824, 941, 1430
ApoI	RAATTY	6 cut(s)	496, 729, 950, 1140, 1284, 1744
AseI	ATTAAT	1 cut(s)	2058
Asp700I	GAANNNNTTC	1 cut(s)	1131
AspLEI	GCGC	5 cut(s)	314, 961, 1368, 2106, 2325
AspS9I	GGNCC	5 cut(s)	595, 760, 1093, 1249, 1705
AsuC2I	CCSGG	1 cut(s)	251
AsuHPI	GGTGA	5 cut(s)	59, 878, 1409, 1454, 2083
AsuII	TTCGAA	1 cut(s)	664
AvaI	CYCGRG	1 cut(s)	857
AvaII	GGWCC	2 cut(s)	1093, 1249
BaeGI	GKGCMC	1 cut(s)	1227
BamHI	GGATCC	1 cut(s)	130
BanI	GGYRCC	1 cut(s)	1224
BanII	GRGCYC	1 cut(s)	2195
BbsI	GAAGAC	2 cut(s)	471, 1272
Bbv12I	GWGCWC	3 cut(s)	357, 378, 2195
BbvI	GCAGC	5 cut(s)	201, 688, 836, 928, 1442
BccI	CCATC	4 cut(s)	302, 787, 1433, 1627
BceAI	ACGGC	2 cut(s)	929, 1132
BciT130I	CCWGG	5 cut(s)	440, 804, 1623, 1739, 1937
BciVI	GTATCC	2 cut(s)	1888, 1970
BclI	TGATCA	2 cut(s)	415, 1722
BcnI	CCSGG	1 cut(s)	251
BcoDI	GTCTC	3 cut(s)	174, 1508, 1514
BfaI	CTAG	4 cut(s)	6, 135, 938, 2217
BfmI	CTRYAG	1 cut(s)	1088
BfoI	RGCGCY	2 cut(s)	315, 1369
BfuAI	ACCTGC	2 cut(s)	1580, 2109
BfuI	GTATCC	2 cut(s)	1888, 1970
BisI	GCNGC	7 cut(s)	190, 702, 825, 929, 942, 1431, 1979
BlsI	GCNGC	7 cut(s)	191, 703, 826, 930, 943, 1432, 1980
BmcAI	AGTACT	1 cut(s)	2111
Bme1390I	CCNGG	6 cut(s)	251, 440, 804, 1623, 1739, 1937
Bme18I	GGWCC	2 cut(s)	1093, 1249
BmeT110I	CYCGRG	1 cut(s)	857
BmgBI	CACGTC	1 cut(s)	341
BmgT120I	GGNCC	5 cut(s)	595, 760, 1093, 1249, 1705
BmiI	GGNNCC	3 cut(s)	132, 761, 1226
BmrFI	CCNGG	6 cut(s)	251, 440, 804, 1623, 1739, 1937
BmsI	GCATC	9 cut(s)	646, 681, 806, 970, 1128, 1417, 1488, 1960, 2097
BpiI	GAAGAC	2 cut(s)	471, 1272
BpmI	CTGGAG	2 cut(s)	786, 1043
Bpu14I	TTCGAA	1 cut(s)	664
BpuEI	CTTGAG	3 cut(s)	492, 1373, 1637
BpuMI	CCSGG	1 cut(s)	251
BsaBI	GATNNNNATC	1 cut(s)	414
BsaHI	GRCGYC	1 cut(s)	1848
BsaJI	CCNNGG	4 cut(s)	828, 858, 999, 1856
BsaXI	ACNNNNNCTCC	2 cut(s)	2182, 2212
Bsc4I	CCNNNNNNNGG	5 cut(s)	126, 218, 863, 1857, 1936
Bse118I	RCCGGY	1 cut(s)	1785
Bse1I	ACTGG	3 cut(s)	1251, 1270, 1569
Bse3DI	GCAATG	1 cut(s)	2001
Bse8I	GATNNNNATC	1 cut(s)	414
BseBI	CCWGG	5 cut(s)	440, 804, 1623, 1739, 1937
BseDI	CCNNGG	4 cut(s)	828, 858, 999, 1856
BseGI	GGATG	6 cut(s)	259, 367, 661, 1042, 1492, 2069
BseJI	GATNNNNATC	1 cut(s)	414
BseLI	CCNNNNNNNGG	5 cut(s)	126, 218, 863, 1857, 1936
BseMI	GCAATG	1 cut(s)	2001
BseMII	CTCAG	6 cut(s)	114, 204, 729, 1467, 2178, 2214
BseNI	ACTGG	3 cut(s)	1251, 1270, 1569
BseRI	GAGGAG	2 cut(s)	1505, 1598
BseSI	GKGCMC	1 cut(s)	1227
BseXI	GCAGC	5 cut(s)	201, 688, 836, 928, 1442
BseYI	CCCAGC	1 cut(s)	1228
Bsh1236I	CGCG	1 cut(s)	2106
Bsh1285I	CGRYCG	1 cut(s)	518
BshFI	GGCC	5 cut(s)	596, 762, 1337, 1707, 1871
BshNI	GGYRCC	1 cut(s)	1224
BsiEI	CGRYCG	1 cut(s)	518
BsiHKAI	GWGCWC	3 cut(s)	357, 378, 2195
BsiHKCI	CYCGRG	1 cut(s)	857
BsiSI	CCGG	2 cut(s)	250, 1786
BslFI	GGGAC	2 cut(s)	2279, 2318
BslI	CCNNNNNNNGG	5 cut(s)	126, 218, 863, 1857, 1936
BsmAI	GTCTC	3 cut(s)	174, 1508, 1514
BsmFI	GGGAC	2 cut(s)	2279, 2318
BsmI	GAATGC	3 cut(s)	496, 541, 908
BsnI	GGCC	5 cut(s)	596, 762, 1337, 1707, 1871
BsoBI	CYCGRG	1 cut(s)	857
Bsp119I	TTCGAA	1 cut(s)	664
Bsp1286I	GDGCHC	4 cut(s)	357, 378, 1227, 2195
BspACI	CCGC	4 cut(s)	916, 929, 1978, 2047
BspANI	GGCC	5 cut(s)	596, 762, 1337, 1707, 1871
BspCNI	CTCAG	6 cut(s)	115, 205, 730, 1468, 2177, 2215
BspFNI	CGCG	1 cut(s)	2106
BspHI	TCATGA	2 cut(s)	985, 1147
BspLI	GGNNCC	3 cut(s)	132, 761, 1226
BspMAI	CTGCAG	1 cut(s)	1092
BspMI	ACCTGC	2 cut(s)	1580, 2109
BspPI	GGATC	9 cut(s)	125, 138, 768, 1087, 1334, 1774, 1921, 1982, 2221
BspQI	GCTCTTC	2 cut(s)	366, 2254
BspT104I	TTCGAA	1 cut(s)	664
BspT107I	GGYRCC	1 cut(s)	1224
BsrBI	CCGCTC	1 cut(s)	1978
BsrDI	GCAATG	1 cut(s)	2001
BsrFI	RCCGGY	1 cut(s)	1785
BsrI	ACTGG	3 cut(s)	1251, 1270, 1569
BssAI	RCCGGY	1 cut(s)	1785
BssECI	CCNNGG	4 cut(s)	828, 858, 999, 1856
BssNI	GRCGYC	1 cut(s)	1848
BssT1I	CCWWGG	3 cut(s)	828, 999, 1856
Bst2UI	CCWGG	5 cut(s)	440, 804, 1623, 1739, 1937
Bst4CI	ACNGT	6 cut(s)	470, 516, 1015, 1043, 1641, 1770
Bst6I	CTCTTC	5 cut(s)	366, 1290, 1476, 1884, 2254
BstACI	GRCGYC	1 cut(s)	1848
BstBI	TTCGAA	1 cut(s)	664
BstDEI	CTNAG	8 cut(s)	75, 123, 213, 738, 1476, 1538, 2164, 2223
BstENI	CCTNNNNNAGG	1 cut(s)	1934
BstF5I	GGATG	6 cut(s)	259, 367, 661, 1042, 1492, 2069
BstFNI	CGCG	1 cut(s)	2106
BstH2I	RGCGCY	2 cut(s)	315, 1369
BstHHI	GCGC	5 cut(s)	314, 961, 1368, 2106, 2325
BstMAI	GTCTC	3 cut(s)	174, 1508, 1514
BstMCI	CGRYCG	1 cut(s)	518
BstMWI	GCNNNNNNNGC	5 cut(s)	545, 768, 777, 833, 1975
BstNI	CCWGG	5 cut(s)	440, 804, 1623, 1739, 1937
BstNSI	RCATGY	1 cut(s)	1865
BstSCI	CCNGG	6 cut(s)	249, 438, 802, 1621, 1737, 1935
BstSFI	CTRYAG	1 cut(s)	1088
BstSLI	GKGCMC	1 cut(s)	1227
BstUI	CGCG	1 cut(s)	2106
BstV1I	GCAGC	5 cut(s)	201, 688, 836, 928, 1442
BstV2I	GAAGAC	2 cut(s)	471, 1272
BstX2I	RGATCY	4 cut(s)	130, 1926, 1987, 2213
BstYI	RGATCY	4 cut(s)	130, 1926, 1987, 2213
BsuI	GTATCC	2 cut(s)	1888, 1970
BsuRI	GGCC	5 cut(s)	596, 762, 1337, 1707, 1871
BtrI	CACGTC	1 cut(s)	341
BtsCI	GGATG	6 cut(s)	259, 367, 661, 1042, 1492, 2069
BtsI	GCAGTG	1 cut(s)	634
BtsIMutI	CAGTG	7 cut(s)	120, 634, 810, 1011, 1048, 1068, 1576
BveI	ACCTGC	2 cut(s)	1580, 2109
CaiI	CAGNNNCTG	2 cut(s)	646, 1991
CciI	TCATGA	2 cut(s)	985, 1147
CfoI	GCGC	5 cut(s)	314, 961, 1368, 2106, 2325
Cfr10I	RCCGGY	1 cut(s)	1785
Cfr13I	GGNCC	5 cut(s)	595, 760, 1093, 1249, 1705
CseI	GACGC	3 cut(s)	80, 1856, 2283
Csp6I	GTAC	1 cut(s)	2110
CviQI	GTAC	1 cut(s)	2110
DdeI	CTNAG	8 cut(s)	75, 123, 213, 738, 1476, 1538, 2164, 2223
Eam1104I	CTCTTC	5 cut(s)	366, 1290, 1476, 1884, 2254
EarI	CTCTTC	5 cut(s)	366, 1290, 1476, 1884, 2254
Ecl136II	GAGCTC	1 cut(s)	2193
Eco130I	CCWWGG	3 cut(s)	828, 999, 1856
Eco24I	GRGCYC	1 cut(s)	2195
Eco47I	GGWCC	2 cut(s)	1093, 1249
Eco47III	AGCGCT	1 cut(s)	1367
Eco53kI	GAGCTC	1 cut(s)	2193
Eco57I	CTGAAG	5 cut(s)	1400, 1419, 1428, 1625, 1908
Eco88I	CYCGRG	1 cut(s)	857
EcoICRI	GAGCTC	1 cut(s)	2193
EcoNI	CCTNNNNNAGG	1 cut(s)	1934
EcoO109I	RGGNCCY	2 cut(s)	760, 1093
EcoRI	GAATTC	1 cut(s)	1140
EcoRII	CCWGG	5 cut(s)	438, 802, 1621, 1737, 1935
EcoT14I	CCWWGG	3 cut(s)	828, 999, 1856
EcoT38I	GRGCYC	1 cut(s)	2195
ErhI	CCWWGG	3 cut(s)	828, 999, 1856
FalI	AAGNNNNNCTT	4 cut(s)	1305, 1337, 1377, 1409
FaqI	GGGAC	2 cut(s)	2279, 2318
FauI	CCCGC	1 cut(s)	2054
FauNDI	CATATG	1 cut(s)	2121
FbaI	TGATCA	2 cut(s)	415, 1722
Fnu4HI	GCNGC	7 cut(s)	190, 702, 825, 929, 942, 1431, 1979
FokI	GGATG	6 cut(s)	266, 374, 668, 1049, 1499, 2056
FriOI	GRGCYC	1 cut(s)	2195
Fsp4HI	GCNGC	7 cut(s)	190, 702, 825, 929, 942, 1431, 1979
FspBI	CTAG	4 cut(s)	6, 135, 938, 2217
GlaI	GCGC	5 cut(s)	313, 960, 1367, 2105, 2324
GluI	GCNGC	7 cut(s)	190, 702, 825, 929, 942, 1431, 1979
GsaI	CCCAGC	1 cut(s)	1232
GsuI	CTGGAG	2 cut(s)	786, 1043
HaeII	RGCGCY	2 cut(s)	315, 1369
HaeIII	GGCC	5 cut(s)	596, 762, 1337, 1707, 1871
HapII	CCGG	2 cut(s)	250, 1786
HgaI	GACGC	3 cut(s)	80, 1856, 2283
HhaI	GCGC	5 cut(s)	314, 961, 1368, 2106, 2325
Hin1I	GRCGYC	1 cut(s)	1848
Hin6I	GCGC	5 cut(s)	312, 959, 1366, 2104, 2323
HinP1I	GCGC	5 cut(s)	312, 959, 1366, 2104, 2323
HincII	GTYRAC	3 cut(s)	844, 970, 1275
HindII	GTYRAC	3 cut(s)	844, 970, 1275
HpaII	CCGG	2 cut(s)	250, 1786
HphI	GGTGA	5 cut(s)	59, 878, 1409, 1454, 2083
Hpy166II	GTNNAC	9 cut(s)	106, 120, 574, 688, 844, 869, 970, 1275, 1735
Hpy8I	GTNNAC	9 cut(s)	106, 120, 574, 688, 844, 869, 970, 1275, 1735
HpyAV	CCTTC	8 cut(s)	155, 417, 452, 518, 671, 750, 1412, 1862
HpyCH4III	ACNGT	6 cut(s)	470, 516, 1015, 1043, 1641, 1770
HpyCH4IV	ACGT	2 cut(s)	289, 340
HpyF10VI	GCNNNNNNNGC	5 cut(s)	545, 768, 777, 833, 1975
HpyF3I	CTNAG	8 cut(s)	75, 123, 213, 738, 1476, 1538, 2164, 2223
HpySE526I	ACGT	2 cut(s)	289, 340
Hsp92I	GRCGYC	1 cut(s)	1848
HspAI	GCGC	5 cut(s)	312, 959, 1366, 2104, 2323
Ksp22I	TGATCA	2 cut(s)	415, 1722
LguI	GCTCTTC	2 cut(s)	366, 2254
LmnI	GCTCC	4 cut(s)	1492, 1605, 1948, 2190
Lsp1109I	GCAGC	5 cut(s)	201, 688, 836, 928, 1442
LweI	GCATC	9 cut(s)	646, 681, 806, 970, 1128, 1417, 1488, 1960, 2097
MaeI	CTAG	4 cut(s)	6, 135, 938, 2217
MaeII	ACGT	2 cut(s)	289, 340
MaeIII	GTNAC	3 cut(s)	341, 1066, 1315
MbiI	CCGCTC	1 cut(s)	1978
MflI	RGATCY	4 cut(s)	130, 1926, 1987, 2213
MhlI	GDGCHC	4 cut(s)	357, 378, 1227, 2195
MlyI	GAGTC	4 cut(s)	2012, 2124, 2156, 2234
MmeI	TCCRAC	1 cut(s)	1926
MroXI	GAANNNNTTC	1 cut(s)	1131
MseI	TTAA	6 cut(s)	588, 900, 948, 1611, 2058, 2338
MslI	CAYNNNNRTG	5 cut(s)	99, 276, 360, 388, 815
MspA1I	CMGCKG	1 cut(s)	1232
MspI	CCGG	2 cut(s)	250, 1786
MspR9I	CCNGG	6 cut(s)	251, 440, 804, 1623, 1739, 1937
Mva1269I	GAATGC	3 cut(s)	496, 541, 908
MvaI	CCWGG	5 cut(s)	440, 804, 1623, 1739, 1937
MvnI	CGCG	1 cut(s)	2106
MwoI	GCNNNNNNNGC	5 cut(s)	545, 768, 777, 833, 1975
NciI	CCSGG	1 cut(s)	251
NdeI	CATATG	1 cut(s)	2121
NlaIV	GGNNCC	3 cut(s)	132, 761, 1226
NmeAIII	GCCGAG	1 cut(s)	1818
NmuCI	GTSAC	2 cut(s)	341, 1066
NspI	RCATGY	1 cut(s)	1865
NspV	TTCGAA	1 cut(s)	664
OliI	CACNNNNGTG	1 cut(s)	815
PagI	TCATGA	2 cut(s)	985, 1147
PciSI	GCTCTTC	2 cut(s)	366, 2254
PcsI	WCGNNNNNNNCGW	3 cut(s)	337, 1458, 2281
PctI	GAATGC	3 cut(s)	496, 541, 908
PdmI	GAANNNNTTC	1 cut(s)	1131
PfoI	TCCNGGA	1 cut(s)	1935
PkrI	GCNGC	7 cut(s)	191, 703, 826, 930, 943, 1432, 1980
PleI	GAGTC	4 cut(s)	2011, 2124, 2156, 2233
PpsI	GAGTC	4 cut(s)	2011, 2124, 2156, 2233
PpuMI	RGGWCCY	1 cut(s)	1093
PshBI	ATTAAT	1 cut(s)	2058
Psp124BI	GAGCTC	1 cut(s)	2195
Psp5II	RGGWCCY	1 cut(s)	1093
Psp6I	CCWGG	5 cut(s)	438, 802, 1621, 1737, 1935
PspFI	CCCAGC	1 cut(s)	1228
PspGI	CCWGG	5 cut(s)	438, 802, 1621, 1737, 1935
PspN4I	GGNNCC	3 cut(s)	132, 761, 1226
PspPI	GGNCC	5 cut(s)	595, 760, 1093, 1249, 1705
PspPPI	RGGWCCY	1 cut(s)	1093
PsrI	GAACNNNNNNTAC	2 cut(s)	133, 165
PstI	CTGCAG	1 cut(s)	1092
PstNI	CAGNNNCTG	2 cut(s)	646, 1991
PsuI	RGATCY	4 cut(s)	130, 1926, 1987, 2213
PvuII	CAGCTG	1 cut(s)	1232
RsaI	GTAC	1 cut(s)	2111
RsaNI	GTAC	1 cut(s)	2110
RseI	CAYNNNNRTG	5 cut(s)	99, 276, 360, 388, 815
SacI	GAGCTC	1 cut(s)	2195
SapI	GCTCTTC	2 cut(s)	366, 2254
SaqAI	TTAA	6 cut(s)	588, 900, 948, 1611, 2058, 2338
SatI	GCNGC	7 cut(s)	190, 702, 825, 929, 942, 1431, 1979
Sau96I	GGNCC	5 cut(s)	595, 760, 1093, 1249, 1705
ScaI	AGTACT	1 cut(s)	2111
SchI	GAGTC	4 cut(s)	2012, 2124, 2156, 2234
ScrFI	CCNGG	6 cut(s)	251, 440, 804, 1623, 1739, 1937
SduI	GDGCHC	4 cut(s)	357, 378, 1227, 2195
SfaNI	GCATC	9 cut(s)	646, 681, 806, 970, 1128, 1417, 1488, 1960, 2097
SfcI	CTRYAG	1 cut(s)	1088
SfuI	TTCGAA	1 cut(s)	664
SinI	GGWCC	2 cut(s)	1093, 1249
SmiMI	CAYNNNNRTG	5 cut(s)	99, 276, 360, 388, 815
SmlI	CTYRAG	3 cut(s)	507, 1352, 1616
SmoI	CTYRAG	3 cut(s)	507, 1352, 1616
SsiI	CCGC	4 cut(s)	916, 929, 1978, 2047
SspMI	CTAG	4 cut(s)	6, 135, 938, 2217
SstI	GAGCTC	1 cut(s)	2195
StyD4I	CCNGG	6 cut(s)	249, 438, 802, 1621, 1737, 1935
StyI	CCWWGG	3 cut(s)	828, 999, 1856
TaaI	ACNGT	6 cut(s)	470, 516, 1015, 1043, 1641, 1770
TaiI	ACGT	2 cut(s)	292, 343
TaqI	TCGA	1 cut(s)	664
TatI	WGTACW	1 cut(s)	2109
TauI	GCSGC	2 cut(s)	931, 1981
Tru1I	TTAA	6 cut(s)	588, 900, 948, 1611, 2058, 2338
Tru9I	TTAA	6 cut(s)	588, 900, 948, 1611, 2058, 2338
TscAI	CASTG	7 cut(s)	127, 634, 817, 1018, 1048, 1075, 1576
TseFI	GTSAC	2 cut(s)	341, 1066
TseI	GCWGC	5 cut(s)	189, 701, 824, 941, 1430
Tsp45I	GTSAC	2 cut(s)	341, 1066
TspGWI	ACGGA	4 cut(s)	1049, 1219, 1822, 1952
TspRI	CASTG	7 cut(s)	127, 634, 817, 1018, 1048, 1075, 1576
VpaK11BI	GGWCC	2 cut(s)	1093, 1249
VspI	ATTAAT	1 cut(s)	2058
XagI	CCTNNNNNAGG	1 cut(s)	1934
XapI	RAATTY	6 cut(s)	496, 729, 950, 1140, 1284, 1744
XceI	RCATGY	1 cut(s)	1865
XmnI	GAANNNNTTC	1 cut(s)	1131
XspI	CTAG	4 cut(s)	6, 135, 938, 2217
ZrmI	AGTACT	1 cut(s)	2111

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.