Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0002528

Physical Location & Seq

Genomic Coordinates Reverse (-)

1516345 .. 1518360

Gene Structure 2016 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG020300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2016 bp

ATGGCTAGAAAGCACAGTATTGAATTGAGCGATAATGATATGAGGCATAGAAGTAAAAACAATCCCAGGTCGAACTATCGTCTTGAACGTGGCGATGAAATTAATTCCTCAGATTCTGATAGAGGATATTCTACAAAAAGAAAAAATTTTGATAGAAGATGTAGAACAAAGCAAAATGCTGGGATATGGGATGAGCATCATAGGAAGGAATCACGAAATAATGATCGACATAGCAGTTCAAAACCACCTCATGGACGCTCCACATTGCTAGAGGGAAACCGAAACCGAAACCTGAATACTGATGTGTCAAGTTTGGGAAAGAGGGATCGACTCTATGTTCCGCCTTATAAGTTGGCCCGAAAGATGAAAGAGGTCGAAGATAAAAGTAGCATTGAGTATCAGAGGCTGACATGGGATGCCCTAAAAAAAAGCATCAATGTGGTAGTGAACAAGGTCAATGCTGCCAACATAAAGAATATAATCCCTGAGATCTTTTCAGAGAATCTCATTCGCGGTAGGGGCCTCTTCTGCCGATCCATTATGAAGTCACAGATGGCATCTCCGGGATTCACAGACGTATTTGCTTCCTTGGTTGCTGTTGTCAACACCAAGTTTCCTGAGGTCGGCCAGCTTATGTTGAGAAGAATTGTCCTGAAGCTTAAGATAGCATTCAAGCGGAACGACAAGCCACAGCTACTAGCTGCAGTTAAATTCATTGCTCATCTTGTAAACCAACAAGTGGCTCATGAGATTGTAGCATTGGAATTGCTAACTGTACTGCTTGAAAACCCTACAGATGACAGTGTTGAAGTTGCTGTATGGTTTGTGACAGATTGTGGCTCAATGCTACAAGACCTTTCACCGACCGGGCTGCGCCGAATCTTTGATCGGTTTTTGGGGATTCTTCATGAAGGAGAAATTGACAAACGAGTTCAGTTTTTGATTGATGGCTTATTAGCTTTAAGGAAAGGCAAGTTTAATGGTTACCCAGCTGTTCGTCCAGAGTTGGACCTCGTGGAGCAAGAAGACCAGTTCACTCATCCGGTTTCTCTTCAAGATGAGTTAGATCCAGAAGATAGCCTTAATCTCTTCAAGCCGGACCCTGATTTCCTTAAGAATGAGAAGCGCTACGAAGAAGTAAAGAAAACAATACTTGGTGATGAGTCTGAAGACGACGATGAGGTCAACGACGATGATGAATCTGAAGAAGAAGAAGAGGAGGATGAGCAGCACATGCAAATAAGAGATGAGACAGACACAAATCTCATCAATCTTCGAAGGACGATATATCTGACCATCATGTGTAGTCTAGATTTTGAGGAAGCAGGTCATAAGCTTCTTCAGATTAAGCTCGAACCAGGCCAAGAAATGGAGCTATGCACTATGCTTTTAGAATGTTGCAGTCAAGAGAAAAACTATCGTCCTTTTTACTGTCTCTTGGGACAGCGGCTCTGCATGATCAACAGAATCTACCGAGAAAATTTTGAGAGATGTTTTGTACAGCAGTACTCGATGATCCACCAGTTTGAAACAAACAAGATTCGCAATGTTGCTTCATTTTACGCCCATTTACTCTCAACAGATGCTCTTCCATGGCATTGCATAGCCTATATGCGTCTAACCGAAAAGGACACCACTTCGTCTTCTAGGATTTTTATCAAGATCCTTTTCCAGGAGTTGTCAAAGCAGCTTGGCACACGTATGCTTAATGAGCGGCTCCATGATCCGGCCATGCGAGAAAGTAACGAGTCTATTTTGCCGAGGGATAATCCAAGGAACTCCCGGTTCGCCATAAATTTCTTCACACAAATAGGGCTTGGTCCTCTCACTGAGAACCTGCGTGAGTTCTATGATCACCTGCCACAGCTTATTAGGCAACAACAAAAAGCAGTGTTGGAGGAAGAATCTGAGAGCTCTGGTTCATCGGATTCGGAATCGGAATCATCAAGCTCTGATGATGAAAGCGAAAGTGATGGGGATGAAAGACGCAAGAATAAACGTAGGAGGAGAGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0071006 GO:0071012 GO:0071013 GO:0071704 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MA3 MIF4G

Protein Analysis

671

Amino Acids

78.12

Weight (kDa)

5.79

Isoelectric Point (pI)

56.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MIF4G	PF02854	147 - 323	6.3e-13	MIF4G domain
MA3	PF02847	425 - 531	4.7e-28	MA3 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002528)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G80930
fragaria_vesca	FvH4_3g01450 FvH4_3g43230 FvH4_3g43240 FvH4_3g43240
malus_domestica	MD03G1023500.v1.1 MD11G1028600.v1.1
prunus_persica	Prupe.6G022700_v2.0.a1 Prupe.6G022700_v2.0.a1
pyrus_communis	pycom03g02010 pycom11g02210
rosa_chinensis	RchiOBHm_Chr5g0002291 RchiOBHm_Chr5g0077281 RchiOBHm_Chr5g0077291
rosa_laevigata	RLG00000031014 RLG00000036681 RLG00000036682
rosa_multiflora	Rmu_co8446135.1_g000001 Rmu_sc0000433.1_g000042 Rmu_sc0001492.1_g000008 Rmu_sc0005576.1_g000001 Rmu_sc0006137.1_g000009 Rmu_sc0008383.1_g000001 Rmu_sc0013155.1_g000012 Rmu_ssc0000410.1_g000040
rosa_roxburghii	Rroxscaffold_1G00004680 Rroxscaffold_1G00073650
rosa_rugosa	Rorug04G0394900 Rorug05G0453500
rosa_samantha	Rh5AG017700 Rh5AG508400 Rh5AG508500 Rh5BG020300 Rh5BG529600 Rh5CG019100 Rh5CG019400 Rh5CG554100 Rh5DG018300 Rh5DG542200
rosa_wichuraiana	Rw5G046900 Rw5G046910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	348
AarI	CACCTGC	1 cut(s)	1866
AasI	GACNNNNNNGTC	1 cut(s)	1181
Acc36I	ACCTGC	3 cut(s)	1316, 1845, 1866
AccB7I	CCANNNNNTGG	3 cut(s)	251, 739, 1369
AccBSI	CCGCTC	1 cut(s)	1714
AccII	CGCG	1 cut(s)	513
AciI	CCGC	5 cut(s)	341, 513, 676, 1447, 1714
AclWI	GGATC	6 cut(s)	333, 528, 1061, 1510, 1657, 1718
AcoI	YGGCCR	2 cut(s)	625, 1728
AcsI	RAATTY	4 cut(s)	145, 710, 1480, 1795
AcuI	CTGAAG	4 cut(s)	674, 1188, 1224, 1325
AfaI	GTAC	3 cut(s)	777, 1500, 1508
AfeI	AGCGCT	1 cut(s)	1127
AfiI	CCNNNNNNNGG	6 cut(s)	251, 623, 739, 1369, 1672, 1726
AflII	CTTAAG	2 cut(s)	659, 1112
AflIII	ACRYGT	1 cut(s)	1697
AgsI	TTSAA	9 cut(s)	23, 86, 240, 673, 785, 809, 1055, 1093, 1529
AjnI	CCWGG	3 cut(s)	65, 1357, 1671
Alw21I	GWGCWC	1 cut(s)	1916
Alw26I	GTCTC	2 cut(s)	1244, 1439
AlwI	GGATC	6 cut(s)	333, 528, 1061, 1510, 1657, 1718
AlwNI	CAGNNNCTG	2 cut(s)	116, 406
Aor51HI	AGCGCT	1 cut(s)	1127
AoxI	GGCC	5 cut(s)	354, 520, 625, 1360, 1728
ApeKI	GCWGC	5 cut(s)	461, 701, 871, 1228, 1687
ApoI	RAATTY	4 cut(s)	145, 710, 1480, 1795
AseI	ATTAAT	1 cut(s)	102
AspLEI	GCGC	2 cut(s)	876, 1128
AspS9I	GGNCC	5 cut(s)	355, 520, 1009, 1099, 1820
AsuC2I	CCSGG	3 cut(s)	564, 868, 1783
AsuHPI	GGTGA	3 cut(s)	852, 1169, 1847
AsuII	TTCGAA	1 cut(s)	1276
AvaII	GGWCC	3 cut(s)	1009, 1099, 1820
AxyI	CCTNAGG	1 cut(s)	618
BanII	GRGCYC	1 cut(s)	1916
BauI	CACGAG	1 cut(s)	1013
BbsI	GAAGAC	3 cut(s)	1032, 1176, 1635
Bbv12I	GWGCWC	1 cut(s)	1916
BbvI	GCAGC	5 cut(s)	448, 688, 858, 1240, 1699
BccI	CCATC	4 cut(s)	547, 941, 1304, 1967
BcgI	CGANNNNNNTGC	2 cut(s)	853, 887
BciT130I	CCWGG	3 cut(s)	67, 1359, 1673
BclI	TGATCA	2 cut(s)	1458, 1852
BcnI	CCSGG	3 cut(s)	564, 868, 1783
BcoDI	GTCTC	2 cut(s)	1244, 1439
BfaI	CTAG	5 cut(s)	6, 269, 698, 1310, 1647
BfmI	CTRYAG	2 cut(s)	702, 792
BfoI	RGCGCY	1 cut(s)	1129
BfrI	CTTAAG	2 cut(s)	659, 1112
BfuAI	ACCTGC	3 cut(s)	1316, 1845, 1866
BglII	AGATCT	1 cut(s)	489
BisI	GCNGC	7 cut(s)	462, 702, 872, 1229, 1448, 1688, 1715
BlsI	GCNGC	7 cut(s)	463, 703, 873, 1230, 1449, 1689, 1716
BmcAI	AGTACT	1 cut(s)	1508
Bme1390I	CCNGG	6 cut(s)	67, 564, 868, 1359, 1673, 1783
Bme18I	GGWCC	3 cut(s)	1009, 1099, 1820
BmgT120I	GGNCC	5 cut(s)	355, 520, 1009, 1099, 1820
BmiI	GGNNCC	3 cut(s)	521, 1101, 1718
BmrFI	CCNGG	6 cut(s)	67, 564, 868, 1359, 1673, 1783
BmsI	GCATC	5 cut(s)	205, 406, 441, 566, 1573
BpiI	GAAGAC	3 cut(s)	1032, 1176, 1635
Bpu14I	TTCGAA	1 cut(s)	1276
BpuMI	CCSGG	3 cut(s)	564, 868, 1783
BsaAI	YACGTR	1 cut(s)	1700
BsaBI	GATNNNNATC	2 cut(s)	195, 1655
BsaJI	CCNNGG	5 cut(s)	65, 588, 1592, 1760, 1772
BsaWI	WCCGGW	1 cut(s)	1042
Bsc4I	CCNNNNNNNGG	6 cut(s)	251, 623, 739, 1369, 1672, 1726
Bse1I	ACTGG	2 cut(s)	1030, 1522
Bse21I	CCTNAGG	1 cut(s)	618
Bse3DI	GCAATG	4 cut(s)	263, 714, 1552, 1597
Bse8I	GATNNNNATC	2 cut(s)	195, 1655
BseBI	CCWGG	3 cut(s)	67, 1359, 1673
BseDI	CCNNGG	5 cut(s)	65, 588, 1592, 1760, 1772
BseGI	GGATG	5 cut(s)	196, 421, 1039, 1228, 1984
BseJI	GATNNNNATC	2 cut(s)	195, 1655
BseLI	CCNNNNNNNGG	6 cut(s)	251, 623, 739, 1369, 1672, 1726
BseMI	GCAATG	4 cut(s)	263, 714, 1552, 1597
BseMII	CTCAG	5 cut(s)	123, 477, 609, 1821, 1899
BseNI	ACTGG	2 cut(s)	1030, 1522
BseRI	GAGGAG	1 cut(s)	1232
BseXI	GCAGC	5 cut(s)	448, 688, 858, 1240, 1699
BseYI	CCCAGC	2 cut(s)	179, 988
Bsh1236I	CGCG	1 cut(s)	513
Bsh1285I	CGRYCG	1 cut(s)	867
BshFI	GGCC	5 cut(s)	356, 522, 627, 1362, 1730
BsiEI	CGRYCG	1 cut(s)	867
BsiHKAI	GWGCWC	1 cut(s)	1916
BsiSI	CCGG	6 cut(s)	563, 867, 1043, 1097, 1727, 1783
BslFI	GGGAC	1 cut(s)	1455
BslI	CCNNNNNNNGG	6 cut(s)	251, 623, 739, 1369, 1672, 1726
BsmAI	GTCTC	2 cut(s)	1244, 1439
BsmFI	GGGAC	1 cut(s)	1455
BsmI	GAATGC	1 cut(s)	668
BsnI	GGCC	5 cut(s)	356, 522, 627, 1362, 1730
Bsp119I	TTCGAA	1 cut(s)	1276
Bsp1286I	GDGCHC	1 cut(s)	1916
Bsp1407I	TGTACA	1 cut(s)	1498
Bsp19I	CCATGG	1 cut(s)	1592
BspACI	CCGC	5 cut(s)	341, 513, 676, 1447, 1714
BspANI	GGCC	5 cut(s)	356, 522, 627, 1362, 1730
BspCNI	CTCAG	5 cut(s)	122, 478, 610, 1822, 1900
BspFNI	CGCG	1 cut(s)	513
BspHI	TCATGA	2 cut(s)	745, 907
BspLI	GGNNCC	3 cut(s)	521, 1101, 1718
BspMAI	CTGCAG	1 cut(s)	706
BspMI	ACCTGC	3 cut(s)	1316, 1845, 1866
BspPI	GGATC	6 cut(s)	333, 528, 1061, 1510, 1657, 1718
BspQI	GCTCTTC	1 cut(s)	1593
BspT104I	TTCGAA	1 cut(s)	1276
BspTI	CTTAAG	2 cut(s)	659, 1112
BsrBI	CCGCTC	1 cut(s)	1714
BsrDI	GCAATG	4 cut(s)	263, 714, 1552, 1597
BsrGI	TGTACA	1 cut(s)	1498
BsrI	ACTGG	2 cut(s)	1030, 1522
BssECI	CCNNGG	5 cut(s)	65, 588, 1592, 1760, 1772
BssSI	CACGAG	1 cut(s)	1013
BssT1I	CCWWGG	3 cut(s)	588, 1592, 1772
Bst2BI	CACGAG	1 cut(s)	1013
Bst2UI	CCWGG	3 cut(s)	67, 1359, 1673
Bst4CI	ACNGT	4 cut(s)	17, 775, 803, 1433
Bst6I	CTCTTC	5 cut(s)	530, 1056, 1094, 1209, 1593
BstAFI	CTTAAG	2 cut(s)	659, 1112
BstAPI	GCANNNNNTGC	1 cut(s)	1234
BstAUI	TGTACA	1 cut(s)	1498
BstBAI	YACGTR	1 cut(s)	1700
BstBI	TTCGAA	1 cut(s)	1276
BstC8I	GCNNGC	1 cut(s)	629
BstDEI	CTNAG	5 cut(s)	109, 486, 618, 1830, 1908
BstDSI	CCRYGG	1 cut(s)	1592
BstEII	GGTNACC	1 cut(s)	983
BstENI	CCTNNNNNAGG	1 cut(s)	1670
BstF5I	GGATG	5 cut(s)	196, 421, 1039, 1228, 1984
BstFNI	CGCG	1 cut(s)	513
BstH2I	RGCGCY	1 cut(s)	1129
BstHHI	GCGC	2 cut(s)	876, 1128
BstMAI	GTCTC	2 cut(s)	1244, 1439
BstMCI	CGRYCG	1 cut(s)	867
BstMWI	GCNNNNNNNGC	5 cut(s)	519, 528, 1234, 1711, 1873
BstNI	CCWGG	3 cut(s)	67, 1359, 1673
BstNSI	RCATGY	1 cut(s)	1237
BstPI	GGTNACC	1 cut(s)	983
BstSCI	CCNGG	6 cut(s)	65, 562, 866, 1357, 1671, 1781
BstSFI	CTRYAG	2 cut(s)	702, 792
BstUI	CGCG	1 cut(s)	513
BstV1I	GCAGC	5 cut(s)	448, 688, 858, 1240, 1699
BstV2I	GAAGAC	3 cut(s)	1032, 1176, 1635
BstX2I	RGATCY	3 cut(s)	489, 1066, 1662
BstYI	RGATCY	3 cut(s)	489, 1066, 1662
Bsu36I	CCTNAGG	1 cut(s)	618
BsuRI	GGCC	5 cut(s)	356, 522, 627, 1362, 1730
BtgI	CCRYGG	1 cut(s)	1592
BtgZI	GCGATG	1 cut(s)	108
BtsCI	GGATG	5 cut(s)	196, 421, 1039, 1228, 1984
BtsI	GCAGTG	1 cut(s)	1896
BtsIMutI	CAGTG	3 cut(s)	808, 1827, 1896
BveI	ACCTGC	3 cut(s)	1316, 1845, 1866
Cac8I	GCNNGC	1 cut(s)	629
CaiI	CAGNNNCTG	2 cut(s)	116, 406
CciI	TCATGA	2 cut(s)	745, 907
CfoI	GCGC	2 cut(s)	876, 1128
Cfr13I	GGNCC	5 cut(s)	355, 520, 1009, 1099, 1820
CseI	GACGC	3 cut(s)	264, 1604, 1995
Csp6I	GTAC	3 cut(s)	776, 1499, 1507
CviQI	GTAC	3 cut(s)	776, 1499, 1507
DdeI	CTNAG	5 cut(s)	109, 486, 618, 1830, 1908
DrdI	GACNNNNNNGTC	1 cut(s)	1181
DseDI	GACNNNNNNGTC	1 cut(s)	1181
EaeI	YGGCCR	2 cut(s)	625, 1728
Eam1104I	CTCTTC	5 cut(s)	530, 1056, 1094, 1209, 1593
EarI	CTCTTC	5 cut(s)	530, 1056, 1094, 1209, 1593
EciI	GGCGGA	1 cut(s)	330
Ecl136II	GAGCTC	1 cut(s)	1914
Eco130I	CCWWGG	3 cut(s)	588, 1592, 1772
Eco24I	GRGCYC	1 cut(s)	1916
Eco47I	GGWCC	3 cut(s)	1009, 1099, 1820
Eco47III	AGCGCT	1 cut(s)	1127
Eco53kI	GAGCTC	1 cut(s)	1914
Eco57I	CTGAAG	4 cut(s)	674, 1188, 1224, 1325
Eco81I	CCTNAGG	1 cut(s)	618
Eco91I	GGTNACC	1 cut(s)	983
EcoICRI	GAGCTC	1 cut(s)	1914
EcoNI	CCTNNNNNAGG	1 cut(s)	1670
EcoO109I	RGGNCCY	1 cut(s)	520
EcoO65I	GGTNACC	1 cut(s)	983
EcoRII	CCWGG	3 cut(s)	65, 1357, 1671
EcoT14I	CCWWGG	3 cut(s)	588, 1592, 1772
EcoT38I	GRGCYC	1 cut(s)	1916
ErhI	CCWWGG	3 cut(s)	588, 1592, 1772
FalI	AAGNNNNNCTT	2 cut(s)	1065, 1097
FaqI	GGGAC	1 cut(s)	1455
FbaI	TGATCA	2 cut(s)	1458, 1852
Fnu4HI	GCNGC	7 cut(s)	462, 702, 872, 1229, 1448, 1688, 1715
FokI	GGATG	5 cut(s)	203, 428, 1026, 1235, 1991
FriOI	GRGCYC	1 cut(s)	1916
Fsp4HI	GCNGC	7 cut(s)	462, 702, 872, 1229, 1448, 1688, 1715
FspBI	CTAG	5 cut(s)	6, 269, 698, 1310, 1647
GlaI	GCGC	2 cut(s)	875, 1127
GluI	GCNGC	7 cut(s)	462, 702, 872, 1229, 1448, 1688, 1715
GsaI	CCCAGC	2 cut(s)	183, 992
HaeII	RGCGCY	1 cut(s)	1129
HaeIII	GGCC	5 cut(s)	356, 522, 627, 1362, 1730
HapII	CCGG	6 cut(s)	563, 867, 1043, 1097, 1727, 1783
HgaI	GACGC	3 cut(s)	264, 1604, 1995
HhaI	GCGC	2 cut(s)	876, 1128
Hin6I	GCGC	2 cut(s)	874, 1126
HinP1I	GCGC	2 cut(s)	874, 1126
HincII	GTYRAC	2 cut(s)	604, 1186
HindII	GTYRAC	2 cut(s)	604, 1186
HindIII	AAGCTT	2 cut(s)	656, 1334
HpaII	CCGG	6 cut(s)	563, 867, 1043, 1097, 1727, 1783
HphI	GGTGA	3 cut(s)	852, 1169, 1847
Hpy166II	GTNNAC	5 cut(s)	448, 604, 730, 1035, 1186
Hpy8I	GTNNAC	5 cut(s)	448, 604, 730, 1035, 1186
Hpy99I	CGWCG	2 cut(s)	1178, 1193
HpyAV	CCTTC	3 cut(s)	199, 905, 1272
HpyCH4III	ACNGT	4 cut(s)	17, 775, 803, 1433
HpyCH4IV	ACGT	4 cut(s)	88, 576, 1699, 1999
HpyCH4V	TGCA	6 cut(s)	704, 1237, 1380, 1401, 1455, 1602
HpyF10VI	GCNNNNNNNGC	5 cut(s)	519, 528, 1234, 1711, 1873
HpyF3I	CTNAG	5 cut(s)	109, 486, 618, 1830, 1908
HpySE526I	ACGT	4 cut(s)	88, 576, 1699, 1999
HspAI	GCGC	2 cut(s)	874, 1126
Ksp22I	TGATCA	2 cut(s)	1458, 1852
LguI	GCTCTTC	1 cut(s)	1593
LmnI	GCTCC	4 cut(s)	263, 1018, 1372, 1722
Lsp1109I	GCAGC	5 cut(s)	448, 688, 858, 1240, 1699
LweI	GCATC	5 cut(s)	205, 406, 441, 566, 1573
MaeI	CTAG	5 cut(s)	6, 269, 698, 1310, 1647
MaeII	ACGT	4 cut(s)	88, 576, 1699, 1999
MaeIII	GTNAC	4 cut(s)	546, 826, 983, 1742
MbiI	CCGCTC	1 cut(s)	1714
MflI	RGATCY	3 cut(s)	489, 1066, 1662
MhlI	GDGCHC	1 cut(s)	1916
MlyI	GAGTC	3 cut(s)	324, 1172, 1757
MmeI	TCCRAC	2 cut(s)	987, 1875
MslI	CAYNNNNRTG	2 cut(s)	437, 1700
MspA1I	CMGCKG	2 cut(s)	992, 1447
MspCI	CTTAAG	2 cut(s)	659, 1112
MspI	CCGG	6 cut(s)	563, 867, 1043, 1097, 1727, 1783
MspR9I	CCNGG	6 cut(s)	67, 564, 868, 1359, 1673, 1783
Mva1269I	GAATGC	1 cut(s)	668
MvaI	CCWGG	3 cut(s)	67, 1359, 1673
MvnI	CGCG	1 cut(s)	513
MwoI	GCNNNNNNNGC	5 cut(s)	519, 528, 1234, 1711, 1873
NciI	CCSGG	3 cut(s)	564, 868, 1783
NcoI	CCATGG	1 cut(s)	1592
NlaIV	GGNNCC	3 cut(s)	521, 1101, 1718
NmeAIII	GCCGAG	1 cut(s)	1785
NmuCI	GTSAC	2 cut(s)	546, 826
NspI	RCATGY	1 cut(s)	1237
NspV	TTCGAA	1 cut(s)	1276
PagI	TCATGA	2 cut(s)	745, 907
PaqCI	CACCTGC	1 cut(s)	1866
PciSI	GCTCTTC	1 cut(s)	1593
PcsI	WCGNNNNNNNCGW	1 cut(s)	85
PctI	GAATGC	1 cut(s)	668
PflMI	CCANNNNNTGG	3 cut(s)	251, 739, 1369
PfoI	TCCNGGA	2 cut(s)	562, 1671
PkrI	GCNGC	7 cut(s)	463, 703, 873, 1230, 1449, 1689, 1716
PleI	GAGTC	3 cut(s)	324, 1171, 1756
PpsI	GAGTC	3 cut(s)	324, 1171, 1756
Ppu21I	YACGTR	1 cut(s)	1700
PshBI	ATTAAT	1 cut(s)	102
PsiI	TTATAA	1 cut(s)	348
Psp124BI	GAGCTC	1 cut(s)	1916
Psp6I	CCWGG	3 cut(s)	65, 1357, 1671
PspEI	GGTNACC	1 cut(s)	983
PspFI	CCCAGC	2 cut(s)	179, 988
PspGI	CCWGG	3 cut(s)	65, 1357, 1671
PspN4I	GGNNCC	3 cut(s)	521, 1101, 1718
PspPI	GGNCC	5 cut(s)	355, 520, 1009, 1099, 1820
PstI	CTGCAG	1 cut(s)	706
PstNI	CAGNNNCTG	2 cut(s)	116, 406
PsuI	RGATCY	3 cut(s)	489, 1066, 1662
PvuII	CAGCTG	1 cut(s)	992
RsaI	GTAC	3 cut(s)	777, 1500, 1508
RsaNI	GTAC	3 cut(s)	776, 1499, 1507
RseI	CAYNNNNRTG	2 cut(s)	437, 1700
SacI	GAGCTC	1 cut(s)	1916
SapI	GCTCTTC	1 cut(s)	1593
SatI	GCNGC	7 cut(s)	462, 702, 872, 1229, 1448, 1688, 1715
Sau96I	GGNCC	5 cut(s)	355, 520, 1009, 1099, 1820
ScaI	AGTACT	1 cut(s)	1508
SchI	GAGTC	3 cut(s)	324, 1172, 1757
ScrFI	CCNGG	6 cut(s)	67, 564, 868, 1359, 1673, 1783
SduI	GDGCHC	1 cut(s)	1916
SfaNI	GCATC	5 cut(s)	205, 406, 441, 566, 1573
SfcI	CTRYAG	2 cut(s)	702, 792
SfuI	TTCGAA	1 cut(s)	1276
SinI	GGWCC	3 cut(s)	1009, 1099, 1820
SmiMI	CAYNNNNRTG	2 cut(s)	437, 1700
SmlI	CTYRAG	2 cut(s)	659, 1112
SmoI	CTYRAG	2 cut(s)	659, 1112
SsiI	CCGC	5 cut(s)	341, 513, 676, 1447, 1714
SspMI	CTAG	5 cut(s)	6, 269, 698, 1310, 1647
SstI	GAGCTC	1 cut(s)	1916
StyD4I	CCNGG	6 cut(s)	65, 562, 866, 1357, 1671, 1781
StyI	CCWWGG	3 cut(s)	588, 1592, 1772
TaaI	ACNGT	4 cut(s)	17, 775, 803, 1433
TaiI	ACGT	4 cut(s)	91, 579, 1702, 2002
TaqI	TCGA	7 cut(s)	71, 226, 328, 375, 1276, 1353, 1511
TatI	WGTACW	3 cut(s)	775, 1498, 1506
TauI	GCSGC	2 cut(s)	1450, 1717
TscAI	CASTG	3 cut(s)	808, 1834, 1896
TseFI	GTSAC	2 cut(s)	546, 826
TseI	GCWGC	5 cut(s)	461, 701, 871, 1228, 1687
Tsp45I	GTSAC	2 cut(s)	546, 826
TspRI	CASTG	3 cut(s)	808, 1834, 1896
Van91I	CCANNNNNTGG	3 cut(s)	251, 739, 1369
Vha464I	CTTAAG	2 cut(s)	659, 1112
VpaK11BI	GGWCC	3 cut(s)	1009, 1099, 1820
VspI	ATTAAT	1 cut(s)	102
XagI	CCTNNNNNAGG	1 cut(s)	1670
XapI	RAATTY	4 cut(s)	145, 710, 1480, 1795
XbaI	TCTAGA	1 cut(s)	1309
XceI	RCATGY	1 cut(s)	1237
XspI	CTAG	5 cut(s)	6, 269, 698, 1310, 1647
ZrmI	AGTACT	1 cut(s)	1508

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.