Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004162

Physical Location & Seq

Genomic Coordinates Forward (+)

7072217 .. 7075673

Gene Structure 3457 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1086500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2436 bp

ATGTTCGCAGCGGCGCCCTCCTCCGCCTACCTCAACCCCAAGCCCACCGTCCTCCCCTCCTCCGCCCGCACCCTTTCCCCGCCCATCACATACGCCGCCGCTTCTAACAGCCGTCGACCAGTCGCTGAATGTCACCTCTCCTCCAATACCCCCTCGCCCCTCTCCCAAAACCCTAAACCTTCCTCCACCTCCCTCAAAACCCTCTCCAAAGCTCTCGCAGCCGCCTCCACAGTCACCCTCATCTTAAATTTGCGATCTTTTTCCGGGAACGGAAATTCCGGTGGTGGCGGAGGTGGGTTTGGAGGCGGAGGTGGAAGGGGAGATGGGTTCGGCGGCGGCGAAGATGGGTTTGGTGGGTTTTTGAAGAGGCTGTTGTTTGGGTCACAGACGGCCATTGCGGACGAGCCTCAGTCCCAAGAGTGGGACTCGCATGGGTTACCGGCCAACATTGTTGTCCAGCTCAACAAGCTCAGTGGGTTCAAGAAATACAAGGTCTCCGAGATCTTGTTCTTCGATCGGCGGCGGTGGAGCACCGTCGGCACCGAAGACTCGTTCTTCGAAATGGTATCCCTGAGACCCGGCGGCGTTTACACCAAAGCCCAGTTGCAGAAGGAGCTGGAAACCTTAGCCAACTGCGGGATGTTCGAGAAGGTCGATTTGGAAGGGAAGACGAACCCAGATGGGACTCTAGGCGTTACAATCTCGTTCACAGAGAGCACATGGCAGTCAGCGGACAAGTTCCGGTGCATCAATGTGGGTCTGATGGCGCAGTCGAAGCCCATTGAAATGGACCCGGACATGACCGATAAGGAAAAGCTCGAGTATTTTCGGAACCAGGAGAAGGATTACAAGAGAAGGATTGATAGGGCAAGGCCTTGTTTGTTGCCGGCGCCTGTGCAGAGGGAAGTGCTGCTGATGTTGAGGGAACAAGGGAAGGTGAGTGCGAGGCTGTTGCAGAAGATTCGAGACCGGGTTCAGAAGTGGTATCAGGATGAAGGGTATGCTTGTGCACAGGTTGTGAATTTTGGGAATTTGAATACCAAGGAGGTGGTTTGTGAGGTTGTGGAAGGGGATATCACCCAGCTGCTTATTCAGTTCCAGGATAAGCTCGGGAATATCGTGGAAGGGAACACTCAGATCCCCGTGGTGAAGAGGGAATTGCCCAGGCAGCTTCGGCCTGGTTATGTTTTTAACATAGAAGCAGGGAAACAAGCTCTGAGGAACATAAATTCCCTAGCTTTGTTTTCAAACATTGAAGTGAACCCACGGCCTGATGAGAAGAACGAAGGAGGGATAATTGTTGAAATAAAGCTAAAAGAACTAGAACAGAAGACAGCTGAACTTAACACGGAATGGAACATTGTTCCTGGACGCGGAGGTTATCCGACTCTGGCTTCACTAGAGCCTGGTGGAACTGTTACATTTGAGCATCGGAATCTCGGTGGCCTGAATAGGTCTATTCTGGGATCAATAACCACCAGCAACTTCTTGAATCCTCAGGATGATCTTTCTTTTAAGCTTGAGTATGTGCATCCATATCTGGATGGCGTGTACAATCCCCGCAATCGTACTCTCCGTGTAAGCTGCTTCAACAGCCGAAAACTAAGTCCAGTCTTTACTGGTGGGCCAGGGGCTGATGAAGTTCCCCCTATTTGGGTTGATCGAGCTGGAGTTAAGGCTAATATAACAGAGAATTTCACCCGTCAGAGCAAATTCACTTATGGACTTGTAATGGAAGAGATAACTACACGTGATGAGCGCAGTCATGTCTGTTCTAATGGCCAAAGAGTATTGCCAAGTGGAGGAGTCAGTGAAGATGGACCTCCAACAACTCTCAGCGGTACAGGCATTGACCGCCTGGCATTTTTACAATCAAATATCACCCGTGATAACACCAAGTTTGTGAATGGAGCTATAGTTGGTCAGAGGAATGTGTTTCAGGTTGACCAAGGCATTGGTGTTGGCAGCAACTTCCCCTTCTTTAACCGTCACCAGCTAACATTAACAAGATTTTTCCAGCTGAAGGAAGTCGAGGAAGGTGCTGGTAAACCACCACCACCCGTGCTTGTACTTCACGGACACTATGGTGGCTGTATAGGAGACCTACCAAGTTATGATGCATTTACCCTTGGCGGTCCATATTCTGTCAGGGGTTACAACATGGGCGAGATAGGTGCAGCTAGAAACATCCTTGAGCTTGCTGCTGAACTACGGATACCCGTGAAAGGTACACATGTATATGCATTTGCAGAGCACGGAAATGATCTAGGAACCTCCAAGGAAGTCAAAGGTAACCCTACAGAGGTATACAGGCGATTGGGTCACGGGTCAGCATATGGCGTTGGCGTCAAGTTAGGTTTAGTAAGAGCTGAGTATGCCGTCGATCACAACTCTGGCACAGGTGCAGTATTCTTCCGTTTCGGCGAGAGGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006605 GO:0006810 GO:0006886 GO:0006996 GO:0007275 GO:0008104 GO:0008150 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009653 GO:0009657 GO:0009658 GO:0009662 GO:0009707 GO:0009719 GO:0009725 GO:0009735 GO:0009790 GO:0009941 GO:0009987 GO:0010006 GO:0010033 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016043 GO:0017038 GO:0019867 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031350 GO:0031351 GO:0031354 GO:0031355 GO:0031358 GO:0031359 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0042170 GO:0042221 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0048598 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0061927 GO:0065002 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072596 GO:0072598 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Bac_surface_Ag

Protein Analysis

812

Amino Acids

88.7

Weight (kDa)

8.61

Isoelectric Point (pI)

44.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
POTRA1_3_Toc75	PF25282	147 - 238	8.4e-41	Toc75-like, POTRA domain
POTRA2_Toc75	PF25280	247 - 358	9.7e-59	Toc75-like, second POTRA domain
POTRA1_3_Toc75	PF25282	359 - 440	5.7e-30	Toc75-like, POTRA domain
Omp85	PF01103	479 - 811	3.2e-21	Omp85 superfamily domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004162)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46740 AT4G09080 AT4G09080 AT4G09080
fragaria_vesca	FvH4_3g28760 FvH4_3g28760 FvH4_3g28760 FvH4_5g29410
malus_domestica	MD03G1086500.v1.1 MD11G1095600.v1.1
prunus_persica	Prupe.6G071100_v2.0.a1
pyrus_communis	pycom03g06860 pycom11g08100 pycom11g08110
rosa_chinensis	RchiOBHm_Chr5g0052291 RchiOBHm_Chr7g0222311
rosa_laevigata	RLG00000002071 RLG00000034828
rosa_multiflora	Rmu_sc0007069.1_g000009 Rmu_sc0028377.1_g000001 Rmu_sc0032875.1_g000001 Rmu_sc0032876.1_g000001
rosa_roxburghii	Rroxscaffold_1G00028120 Rroxscaffold_3G00237120
rosa_rugosa	Rorug05G0273100 Rorug07G0207600
rosa_samantha	Rh5CG382000 Rh5DG371900 Rh7AG349900 Rh7DG346100
rosa_wichuraiana	Rw5G032630 Rw5G032670 Rw7G029530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	13, 539, 889
AccI	GTMKAC	2 cut(s)	115, 2307
AccII	CGCG	1 cut(s)	1374
AclWI	GGATC	2 cut(s)	1132, 1474
AcoI	YGGCCR	3 cut(s)	390, 441, 1780
AcsI	RAATTY	7 cut(s)	247, 274, 1021, 1030, 1228, 1693, 1712
AcuI	CTGAAG	1 cut(s)	2042
AcvI	CACGTG	1 cut(s)	1751
AcyI	GRCGYC	3 cut(s)	14, 890, 2346
AfaI	GTAC	5 cut(s)	1553, 1570, 1843, 2070, 2230
AflIII	ACRYGT	2 cut(s)	1748, 2233
AgsI	TTSAA	9 cut(s)	364, 481, 785, 1036, 1248, 1256, 1304, 1492, 1591
AjnI	CCWGG	8 cut(s)	834, 1098, 1163, 1177, 1366, 1405, 1627, 1857
AjuI	GAANNNNNNNTTGG	4 cut(s)	333, 365, 641, 673
AleI	CACNNNNGTG	1 cut(s)	2085
AloI	GAACNNNNNNTCC	4 cut(s)	260, 292, 1214, 1246
Alw21I	GWGCWC	4 cut(s)	533, 719, 1012, 2256
Alw26I	GTCTC	4 cut(s)	499, 568, 960, 2094
Alw44I	GTGCAC	1 cut(s)	1008
AlwI	GGATC	2 cut(s)	1132, 1474
AlwNI	CAGNNNCTG	2 cut(s)	125, 1634
Ama87I	CYCGRG	2 cut(s)	818, 1109
AoxI	GGCC	8 cut(s)	390, 441, 872, 1175, 1268, 1444, 1625, 1780
ApaLI	GTGCAC	1 cut(s)	1008
ApeKI	GCWGC	9 cut(s)	8, 218, 910, 1084, 1168, 1584, 1965, 2177, 2201
ApoI	RAATTY	7 cut(s)	247, 274, 1021, 1030, 1228, 1693, 1712
ArsI	GACNNNNNNTTYG	4 cut(s)	539, 571, 1591, 1623
AspLEI	GCGC	4 cut(s)	16, 769, 892, 1761
AspS9I	GGNCC	4 cut(s)	790, 1625, 1820, 2135
AsuC2I	CCSGG	4 cut(s)	265, 579, 794, 971
AsuHPI	GGTGA	8 cut(s)	125, 226, 949, 1069, 1159, 1690, 1873, 1982
AsuII	TTCGAA	1 cut(s)	558
AvaI	CYCGRG	2 cut(s)	818, 1109
AvaII	GGWCC	3 cut(s)	790, 1820, 2135
AxyI	CCTNAGG	1 cut(s)	1497
BaeGI	GKGCMC	1 cut(s)	1012
BaeI	ACNNNNGTAYC	2 cut(s)	2220, 2253
BalI	TGGCCA	1 cut(s)	1782
BanI	GGYRCC	3 cut(s)	13, 539, 889
BbrPI	CACGTG	1 cut(s)	1751
BbsI	GAAGAC	3 cut(s)	552, 674, 1337
Bbv12I	GWGCWC	4 cut(s)	533, 719, 1012, 2256
BbvI	GCAGC	9 cut(s)	20, 230, 897, 1071, 1180, 1571, 1977, 2188, 2189
BccI	CCATC	7 cut(s)	92, 317, 338, 674, 757, 1538, 1811
BceAI	ACGGC	4 cut(s)	96, 405, 1283, 2363
BcgI	CGANNNNNNTGC	8 cut(s)	934, 944, 968, 978, 2021, 2055, 2156, 2190
BciT130I	CCWGG	8 cut(s)	836, 1100, 1165, 1179, 1368, 1407, 1629, 1859
BciVI	GTATCC	2 cut(s)	577, 2208
BcnI	CCSGG	4 cut(s)	265, 579, 794, 971
BcoDI	GTCTC	4 cut(s)	499, 568, 960, 2094
BfaI	CTAG	6 cut(s)	689, 1235, 1322, 1400, 2181, 2267
BfmI	CTRYAG	2 cut(s)	1914, 2298
BfoI	RGCGCY	2 cut(s)	17, 893
BfuI	GTATCC	2 cut(s)	577, 2208
BglII	AGATCT	1 cut(s)	501
Bme18I	GGWCC	3 cut(s)	790, 1820, 2135
BmeT110I	CYCGRG	2 cut(s)	818, 1109
BmgT120I	GGNCC	4 cut(s)	790, 1625, 1820, 2135
BmiI	GGNNCC	6 cut(s)	15, 541, 792, 833, 891, 2272
BmrI	ACTGGG	1 cut(s)	595
BmsI	GCATC	4 cut(s)	756, 1438, 1540, 2107
BmuI	ACTGGG	1 cut(s)	595
BpiI	GAAGAC	3 cut(s)	552, 674, 1337
BplI	GAGNNNNNCTC	2 cut(s)	410, 442
BpmI	CTGGAG	1 cut(s)	1689
Bpu10I	CCTNAGC	1 cut(s)	625
Bpu14I	TTCGAA	1 cut(s)	558
BpuEI	CTTGAG	2 cut(s)	1541, 2213
BpuMI	CCSGG	4 cut(s)	265, 579, 794, 971
BsaAI	YACGTR	1 cut(s)	1751
BsaHI	GRCGYC	3 cut(s)	14, 890, 2346
BsaI	GGTCTC	4 cut(s)	499, 568, 960, 2094
BsaJI	CCNNGG	8 cut(s)	1041, 1143, 1163, 1265, 1628, 1948, 2128, 2277
BsaWI	WCCGGW	2 cut(s)	278, 741
BsaXI	ACNNNNNCTCC	6 cut(s)	125, 155, 479, 509, 1902, 1932
Bse118I	RCCGGY	2 cut(s)	439, 886
Bse1I	ACTGG	4 cut(s)	119, 601, 1610, 1624
Bse21I	CCTNAGG	1 cut(s)	1497
Bse3DI	GCAATG	1 cut(s)	393
BseBI	CCWGG	8 cut(s)	836, 1100, 1165, 1179, 1368, 1407, 1629, 1859
BseDI	CCNNGG	8 cut(s)	1041, 1143, 1163, 1265, 1628, 1948, 2128, 2277
BseGI	GGATG	6 cut(s)	645, 997, 1507, 1531, 1549, 2187
BseMI	GCAATG	1 cut(s)	393
BseMII	CTCAG	8 cut(s)	422, 484, 563, 1148, 1208, 1511, 1849, 2361
BseNI	ACTGG	4 cut(s)	119, 601, 1610, 1624
BseRI	GAGGAG	4 cut(s)	10, 49, 130, 1818
BseSI	GKGCMC	1 cut(s)	1012
BseXI	GCAGC	9 cut(s)	20, 230, 897, 1071, 1180, 1571, 1977, 2188, 2189
BseYI	CCCAGC	1 cut(s)	1080
BsgI	GTGCAG	3 cut(s)	917, 2196, 2424
Bsh1236I	CGCG	1 cut(s)	1374
Bsh1285I	CGRYCG	1 cut(s)	517
BshFI	GGCC	8 cut(s)	392, 443, 874, 1177, 1270, 1446, 1627, 1782
BshNI	GGYRCC	3 cut(s)	13, 539, 889
BsiEI	CGRYCG	1 cut(s)	517
BsiHKAI	GWGCWC	4 cut(s)	533, 719, 1012, 2256
BsiHKCI	CYCGRG	2 cut(s)	818, 1109
BsiSI	CCGG	8 cut(s)	264, 279, 440, 579, 742, 794, 887, 970
BslFI	GGGAC	3 cut(s)	397, 437, 697
BsmAI	GTCTC	4 cut(s)	499, 568, 960, 2094
BsmFI	GGGAC	3 cut(s)	397, 437, 697
BsnI	GGCC	8 cut(s)	392, 443, 874, 1177, 1270, 1446, 1627, 1782
Bso31I	GGTCTC	4 cut(s)	499, 568, 960, 2094
BsoBI	CYCGRG	2 cut(s)	818, 1109
Bsp119I	TTCGAA	1 cut(s)	558
Bsp1286I	GDGCHC	4 cut(s)	533, 719, 1012, 2256
Bsp1407I	TGTACA	1 cut(s)	1551
Bsp143I	GATC	9 cut(s)	254, 501, 514, 1137, 1466, 1504, 1660, 2263, 2383
BspANI	GGCC	8 cut(s)	392, 443, 874, 1177, 1270, 1446, 1627, 1782
BspCNI	CTCAG	8 cut(s)	421, 483, 564, 1147, 1209, 1510, 1848, 2362
BspFNI	CGCG	1 cut(s)	1374
BspLI	GGNNCC	6 cut(s)	15, 541, 792, 833, 891, 2272
BspPI	GGATC	2 cut(s)	1132, 1474
BspT104I	TTCGAA	1 cut(s)	558
BspT107I	GGYRCC	3 cut(s)	13, 539, 889
BspTNI	GGTCTC	4 cut(s)	499, 568, 960, 2094
BsrDI	GCAATG	1 cut(s)	393
BsrFI	RCCGGY	2 cut(s)	439, 886
BsrGI	TGTACA	1 cut(s)	1551
BsrI	ACTGG	4 cut(s)	119, 601, 1610, 1624
BssAI	RCCGGY	2 cut(s)	439, 886
BssECI	CCNNGG	8 cut(s)	1041, 1143, 1163, 1265, 1628, 1948, 2128, 2277
BssMI	GATC	9 cut(s)	254, 501, 514, 1137, 1466, 1504, 1660, 2263, 2383
BssNAI	GTATAC	1 cut(s)	2308
BssNI	GRCGYC	3 cut(s)	14, 890, 2346
BssT1I	CCWWGG	4 cut(s)	1041, 1948, 2128, 2277
Bst1107I	GTATAC	1 cut(s)	2308
Bst2UI	CCWGG	8 cut(s)	836, 1100, 1165, 1179, 1368, 1407, 1629, 1859
Bst4CI	ACNGT	5 cut(s)	49, 232, 535, 1417, 1988
Bst6I	CTCTTC	3 cut(s)	359, 1145, 1731
BstACI	GRCGYC	3 cut(s)	14, 890, 2346
BstAUI	TGTACA	1 cut(s)	1551
BstBAI	YACGTR	1 cut(s)	1751
BstBI	TTCGAA	1 cut(s)	558
BstC8I	GCNNGC	3 cut(s)	67, 888, 2199
BstDSI	CCRYGG	2 cut(s)	1143, 1265
BstEII	GGTNACC	2 cut(s)	435, 2291
BstF5I	GGATG	6 cut(s)	645, 997, 1507, 1531, 1549, 2187
BstFNI	CGCG	1 cut(s)	1374
BstH2I	RGCGCY	2 cut(s)	17, 893
BstHHI	GCGC	4 cut(s)	16, 769, 892, 1761
BstKTI	GATC	9 cut(s)	257, 504, 517, 1140, 1469, 1507, 1663, 2266, 2386
BstMAI	GTCTC	4 cut(s)	499, 568, 960, 2094
BstMBI	GATC	9 cut(s)	254, 501, 514, 1137, 1466, 1504, 1660, 2263, 2383
BstMCI	CGRYCG	1 cut(s)	517
BstNI	CCWGG	8 cut(s)	836, 1100, 1165, 1179, 1368, 1407, 1629, 1859
BstNSI	RCATGY	1 cut(s)	2237
BstPI	GGTNACC	2 cut(s)	435, 2291
BstSFI	CTRYAG	2 cut(s)	1914, 2298
BstSLI	GKGCMC	1 cut(s)	1012
BstUI	CGCG	1 cut(s)	1374
BstV1I	GCAGC	9 cut(s)	20, 230, 897, 1071, 1180, 1571, 1977, 2188, 2189
BstV2I	GAAGAC	3 cut(s)	552, 674, 1337
BstX2I	RGATCY	2 cut(s)	501, 1137
BstXI	CCANNNNNNTGG	3 cut(s)	787, 1048, 1955
BstYI	RGATCY	2 cut(s)	501, 1137
BstZ17I	GTATAC	1 cut(s)	2308
Bsu36I	CCTNAGG	1 cut(s)	1497
BsuI	GTATCC	2 cut(s)	577, 2208
BsuRI	GGCC	8 cut(s)	392, 443, 874, 1177, 1270, 1446, 1627, 1782
BtgI	CCRYGG	2 cut(s)	1143, 1265
BtsCI	GGATG	6 cut(s)	645, 997, 1507, 1531, 1549, 2187
BtsIMutI	CAGTG	2 cut(s)	478, 1816
Cac8I	GCNNGC	3 cut(s)	67, 888, 2199
CaiI	CAGNNNCTG	2 cut(s)	125, 1634
CfoI	GCGC	4 cut(s)	16, 769, 892, 1761
Cfr10I	RCCGGY	2 cut(s)	439, 886
Cfr13I	GGNCC	4 cut(s)	790, 1625, 1820, 2135
CseI	GACGC	2 cut(s)	1380, 2335
Csp6I	GTAC	5 cut(s)	1552, 1569, 1842, 2069, 2229
CspCI	CAANNNNNGTGG	2 cut(s)	454, 489
CviAII	CATG	6 cut(s)	431, 720, 799, 1766, 2161, 2234
CviQI	GTAC	5 cut(s)	1552, 1569, 1842, 2069, 2229
DinI	GGCGCC	2 cut(s)	15, 891
DpnI	GATC	9 cut(s)	256, 503, 516, 1139, 1468, 1506, 1662, 2265, 2385
DpnII	GATC	9 cut(s)	254, 501, 514, 1137, 1466, 1504, 1660, 2263, 2383
EaeI	YGGCCR	3 cut(s)	390, 441, 1780
Eam1104I	CTCTTC	3 cut(s)	359, 1145, 1731
EarI	CTCTTC	3 cut(s)	359, 1145, 1731
EciI	GGCGGA	4 cut(s)	13, 52, 303, 321
Eco130I	CCWWGG	4 cut(s)	1041, 1948, 2128, 2277
Eco147I	AGGCCT	1 cut(s)	874
Eco31I	GGTCTC	4 cut(s)	499, 568, 960, 2094
Eco32I	GATATC	1 cut(s)	1075
Eco47I	GGWCC	3 cut(s)	790, 1820, 2135
Eco57I	CTGAAG	1 cut(s)	2042
Eco72I	CACGTG	1 cut(s)	1751
Eco81I	CCTNAGG	1 cut(s)	1497
Eco88I	CYCGRG	2 cut(s)	818, 1109
Eco91I	GGTNACC	2 cut(s)	435, 2291
EcoO65I	GGTNACC	2 cut(s)	435, 2291
EcoRII	CCWGG	8 cut(s)	834, 1098, 1163, 1177, 1366, 1405, 1627, 1857
EcoRV	GATATC	1 cut(s)	1075
EcoT14I	CCWWGG	4 cut(s)	1041, 1948, 2128, 2277
EcoT22I	ATGCAT	2 cut(s)	2122, 2245
EgeI	GGCGCC	2 cut(s)	15, 891
EheI	GGCGCC	2 cut(s)	15, 891
ErhI	CCWWGG	4 cut(s)	1041, 1948, 2128, 2277
FaeI	CATG	6 cut(s)	434, 723, 802, 1769, 2164, 2237
FaqI	GGGAC	3 cut(s)	397, 437, 697
FatI	CATG	6 cut(s)	430, 719, 798, 1765, 2160, 2233
FauI	CCCGC	4 cut(s)	74, 87, 629, 1568
FauNDI	CATATG	1 cut(s)	2335
FblI	GTMKAC	2 cut(s)	115, 2307
FokI	GGATG	6 cut(s)	652, 1004, 1514, 1518, 1556, 2174
FspBI	CTAG	6 cut(s)	689, 1235, 1322, 1400, 2181, 2267
GlaI	GCGC	4 cut(s)	15, 768, 891, 1760
GsaI	CCCAGC	1 cut(s)	1084
GsuI	CTGGAG	1 cut(s)	1689
HaeII	RGCGCY	2 cut(s)	17, 893
HaeIII	GGCC	8 cut(s)	392, 443, 874, 1177, 1270, 1446, 1627, 1782
HapII	CCGG	8 cut(s)	264, 279, 440, 579, 742, 794, 887, 970
HgaI	GACGC	2 cut(s)	1380, 2335
HhaI	GCGC	4 cut(s)	16, 769, 892, 1761
Hin1I	GRCGYC	3 cut(s)	14, 890, 2346
Hin1II	CATG	6 cut(s)	434, 723, 802, 1769, 2164, 2237
Hin6I	GCGC	4 cut(s)	14, 767, 890, 1759
HinP1I	GCGC	4 cut(s)	14, 767, 890, 1759
HincII	GTYRAC	2 cut(s)	116, 1945
HindII	GTYRAC	2 cut(s)	116, 1945
HindIII	AAGCTT	1 cut(s)	1517
HinfI	GANTC	8 cut(s)	425, 548, 685, 961, 1387, 1435, 1492, 1806
HpaII	CCGG	8 cut(s)	264, 279, 440, 579, 742, 794, 887, 970
HphI	GGTGA	8 cut(s)	125, 226, 949, 1069, 1159, 1690, 1873, 1982
Hpy188III	TCNNGA	8 cut(s)	481, 646, 965, 989, 1111, 1489, 1499, 1541
Hpy99I	CGWCG	3 cut(s)	117, 539, 2384
HpyCH4III	ACNGT	5 cut(s)	49, 232, 535, 1417, 1988
HpyCH4IV	ACGT	1 cut(s)	1750
HpySE526I	ACGT	1 cut(s)	1750
Hsp92I	GRCGYC	3 cut(s)	14, 890, 2346
Hsp92II	CATG	6 cut(s)	434, 723, 802, 1769, 2164, 2237
HspAI	GCGC	4 cut(s)	14, 767, 890, 1759
KasI	GGCGCC	2 cut(s)	13, 889
KroI	GCCGGC	1 cut(s)	886
KroNI	GCCGGC	1 cut(s)	888
Kzo9I	GATC	9 cut(s)	254, 501, 514, 1137, 1466, 1504, 1660, 2263, 2383
LmnI	GCTCC	3 cut(s)	528, 613, 1910
Lsp1109I	GCAGC	9 cut(s)	20, 230, 897, 1071, 1180, 1571, 1977, 2188, 2189
LweI	GCATC	4 cut(s)	756, 1438, 1540, 2107
MaeI	CTAG	6 cut(s)	689, 1235, 1322, 1400, 2181, 2267
MaeII	ACGT	1 cut(s)	1750
MalI	GATC	9 cut(s)	256, 503, 516, 1139, 1468, 1506, 1662, 2265, 2385
MboI	GATC	9 cut(s)	254, 501, 514, 1137, 1466, 1504, 1660, 2263, 2383
MflI	RGATCY	2 cut(s)	501, 1137
MhlI	GDGCHC	4 cut(s)	533, 719, 1012, 2256
MlsI	TGGCCA	1 cut(s)	1782
MluCI	AATT	9 cut(s)	247, 274, 1021, 1030, 1157, 1228, 1296, 1693, 1712
MluNI	TGGCCA	1 cut(s)	1782
Mly113I	GGCGCC	2 cut(s)	14, 890
MlyI	GAGTC	5 cut(s)	419, 542, 679, 1381, 1815
MmeI	TCCRAC	2 cut(s)	1409, 1850
Mox20I	TGGCCA	1 cut(s)	1782
Mph1103I	ATGCAT	2 cut(s)	2122, 2245
MroNI	GCCGGC	1 cut(s)	886
MscI	TGGCCA	1 cut(s)	1782
MseI	TTAA	7 cut(s)	245, 1191, 1344, 1515, 1674, 1983, 2003
MslI	CAYNNNNRTG	6 cut(s)	752, 785, 1256, 2085, 2238, 2259
Msp20I	TGGCCA	1 cut(s)	1782
MspA1I	CMGCKG	6 cut(s)	11, 731, 1084, 1337, 1839, 2020
MspI	CCGG	8 cut(s)	264, 279, 440, 579, 742, 794, 887, 970
MvaI	CCWGG	8 cut(s)	836, 1100, 1165, 1179, 1368, 1407, 1629, 1859
MvnI	CGCG	1 cut(s)	1374
NaeI	GCCGGC	1 cut(s)	888
NarI	GGCGCC	2 cut(s)	14, 890
NciI	CCSGG	4 cut(s)	265, 579, 794, 971
NdeI	CATATG	1 cut(s)	2335
NdeII	GATC	9 cut(s)	254, 501, 514, 1137, 1466, 1504, 1660, 2263, 2383
NgoMIV	GCCGGC	1 cut(s)	886
NlaIII	CATG	6 cut(s)	434, 723, 802, 1769, 2164, 2237
NlaIV	GGNNCC	6 cut(s)	15, 541, 792, 833, 891, 2272
NmuCI	GTSAC	5 cut(s)	131, 232, 381, 1988, 2321
NsiI	ATGCAT	2 cut(s)	2122, 2245
NspI	RCATGY	1 cut(s)	2237
NspV	TTCGAA	1 cut(s)	558
OliI	CACNNNNGTG	1 cut(s)	2085
PaeR7I	CTCGAG	1 cut(s)	818
PceI	AGGCCT	1 cut(s)	874
PciI	ACATGT	1 cut(s)	2233
PcsI	WCGNNNNNNNCGW	4 cut(s)	336, 651, 1116, 1573
PdiI	GCCGGC	1 cut(s)	888
PfeI	GAWTC	3 cut(s)	961, 1435, 1492
PfoI	TCCNGGA	3 cut(s)	263, 1098, 1366
Ple19I	CGATCG	1 cut(s)	517
PleI	GAGTC	5 cut(s)	419, 542, 679, 1381, 1814
PluTI	GGCGCC	2 cut(s)	17, 893
PmaCI	CACGTG	1 cut(s)	1751
PmlI	CACGTG	1 cut(s)	1751
PpsI	GAGTC	5 cut(s)	419, 542, 679, 1381, 1814
Ppu21I	YACGTR	1 cut(s)	1751
PscI	ACATGT	1 cut(s)	2233
Psp6I	CCWGG	8 cut(s)	834, 1098, 1163, 1177, 1366, 1405, 1627, 1857
PspCI	CACGTG	1 cut(s)	1751
PspEI	GGTNACC	2 cut(s)	435, 2291
PspFI	CCCAGC	1 cut(s)	1080
PspGI	CCWGG	8 cut(s)	834, 1098, 1163, 1177, 1366, 1405, 1627, 1857
PspN4I	GGNNCC	6 cut(s)	15, 541, 792, 833, 891, 2272
PspPI	GGNCC	4 cut(s)	790, 1625, 1820, 2135
PspXI	VCTCGAGB	1 cut(s)	818
PsrI	GAACNNNNNNTAC	2 cut(s)	2199, 2231
PstNI	CAGNNNCTG	2 cut(s)	125, 1634
PsuI	RGATCY	2 cut(s)	501, 1137
PvuI	CGATCG	1 cut(s)	517
PvuII	CAGCTG	3 cut(s)	1084, 1337, 2020
RsaI	GTAC	5 cut(s)	1553, 1570, 1843, 2070, 2230
RsaNI	GTAC	5 cut(s)	1552, 1569, 1842, 2069, 2229
RseI	CAYNNNNRTG	6 cut(s)	752, 785, 1256, 2085, 2238, 2259
SalI	GTCGAC	1 cut(s)	114
SaqAI	TTAA	7 cut(s)	245, 1191, 1344, 1515, 1674, 1983, 2003
Sau3AI	GATC	9 cut(s)	254, 501, 514, 1137, 1466, 1504, 1660, 2263, 2383
Sau96I	GGNCC	4 cut(s)	790, 1625, 1820, 2135
SchI	GAGTC	5 cut(s)	419, 542, 679, 1381, 1815
SduI	GDGCHC	4 cut(s)	533, 719, 1012, 2256
SfaNI	GCATC	4 cut(s)	756, 1438, 1540, 2107
SfcI	CTRYAG	2 cut(s)	1914, 2298
SfoI	GGCGCC	2 cut(s)	15, 891
Sfr274I	CTCGAG	1 cut(s)	818
SfuI	TTCGAA	1 cut(s)	558
SinI	GGWCC	3 cut(s)	790, 1820, 2135
SlaI	CTCGAG	1 cut(s)	818
SmiMI	CAYNNNNRTG	6 cut(s)	752, 785, 1256, 2085, 2238, 2259
SmlI	CTYRAG	3 cut(s)	818, 1520, 2192
SmoI	CTYRAG	3 cut(s)	818, 1520, 2192
Sse9I	AATT	9 cut(s)	247, 274, 1021, 1030, 1157, 1228, 1296, 1693, 1712
SseBI	AGGCCT	1 cut(s)	874
SspDI	GGCGCC	2 cut(s)	13, 889
SspMI	CTAG	6 cut(s)	689, 1235, 1322, 1400, 2181, 2267
StuI	AGGCCT	1 cut(s)	874
StyI	CCWWGG	4 cut(s)	1041, 1948, 2128, 2277
TaaI	ACNGT	5 cut(s)	49, 232, 535, 1417, 1988
TaiI	ACGT	1 cut(s)	1753
TaqII	GACCGA	1 cut(s)	818
TasI	AATT	9 cut(s)	247, 274, 1021, 1030, 1157, 1228, 1296, 1693, 1712
TatI	WGTACW	2 cut(s)	1551, 2068
TauI	GCSGC	8 cut(s)	14, 98, 101, 224, 336, 339, 523, 585
TfiI	GAWTC	3 cut(s)	961, 1435, 1492
Tru1I	TTAA	7 cut(s)	245, 1191, 1344, 1515, 1674, 1983, 2003
Tru9I	TTAA	7 cut(s)	245, 1191, 1344, 1515, 1674, 1983, 2003
TscAI	CASTG	2 cut(s)	478, 1816
TseFI	GTSAC	5 cut(s)	131, 232, 381, 1988, 2321
TseI	GCWGC	9 cut(s)	8, 218, 910, 1084, 1168, 1584, 1965, 2177, 2201
Tsp45I	GTSAC	5 cut(s)	131, 232, 381, 1988, 2321
TspDTI	ATGAA	2 cut(s)	1008, 1653
TspGWI	ACGGA	7 cut(s)	285, 1364, 1565, 2091, 2227, 2271, 2405
TspRI	CASTG	2 cut(s)	478, 1816
VneI	GTGCAC	1 cut(s)	1008
VpaK11BI	GGWCC	3 cut(s)	790, 1820, 2135
XapI	RAATTY	7 cut(s)	247, 274, 1021, 1030, 1228, 1693, 1712
XceI	RCATGY	1 cut(s)	2237
XhoI	CTCGAG	1 cut(s)	818
XmiI	GTMKAC	2 cut(s)	115, 2307
XspI	CTAG	6 cut(s)	689, 1235, 1322, 1400, 2181, 2267
Zsp2I	ATGCAT	2 cut(s)	2122, 2245

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.