Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0004162

Physical Location & Seq

Genomic Coordinates Forward (+)

55396203 .. 55401499

Gene Structure 5297 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G032670.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2436 bp

ATGTTTGCAGCGTCCACCAGCATCTATACCCCCACCACCATCGCTCTCCTCCCTCCCTCCTCCGCTGATCGCAAATTAGCTTCTTTATGCACCGCCACCACCAACAACAACACGCGCACGCGCACGCGCCGCAGTCCAATCAGCAAATGCCGCGTGTCCTCCACCTCCTCGTCCCTTTCTCTAAACCCTAAACCCTCCTCTTCCAACCACCTCCTCAAAAGCCTCGCCAAAACATTCGCCGGCGCCACCGCCGTCACCCTCATCCTAAACCTAACCAATTTTCCCGCCTTCATCGGCGGAGGCGGAGGAGGAGGTCATGGTTCCAATGGAGGCGGCGGCGGCGGCGGAGGAGGAGGGTTTTGGAGGAGGCTGTTTCTCCTGCATGCGGCGAAAGCCGACGAGCCGAACGAGTCGTGGGACTCTCACGGCTTGCCGGGCGACATTGTGGTCCAGCTCAGCAGGCTCAGTGGCTTCAAGAAGTACAAGGTCTCGGAGGTCTTGTTCTTCGACCGGAGGCGGTGGAGCACCGTCGGCGCGGACGACACCATCTCCGACATGGTGTCCCTCAGGCCCGGCGGCGTCTACACCAAGGCCGAGCTCCAGAACGAGCTCCAATCCCTGGCCAATTGCGGAATGTTCGAGAAGGTCGACTTCGAAGGCAAGACCAAGCCGGACGGCACCCTCGCCGTCACCGTCTCGTTCACGGAGAGCACGTGGCAGGCGGCGGAGAGGTTCCGGTGCATTAACGTGGGCCTGATGGCGCAGCCCAAGCCCATTGAGATGGACCCGGACATGACCGAGAGGGAGAAGATCCAGTATTTGAGGAACCAGGAGAGGGATTACAAGAGGAGGATGGAGAGGGCAAGGCCGTGTTTGCTGCCGGCTTCGGTGCAGAGGGAGGTGTTGCAGATGGTGAGAGAGCAGGGGAAGGTGAGTGCTAGGGTGTTGCAGAAGATTCAGGCCCGGATTCTCAAGTGGTACCAAGATGAGGGGTATCTGTGTGCCAATGTTGTCAACTTTGGGAATTTGAATACAAAGGAGGTTGTGTGTGAGGTGGTGGAGGGCGATATTACTCGGCTGCTTATTCAGTTCCGGGATAAGCTTGGCAATGTTGTAGAGGGGAAGACTCAGAGTCTTGTGGTCAAGAGAGAATTGCCCAAACAGCTTCGACCAGGTAATGCATTTAACATAGAAGCAGGGAAACAAGCTCTGAGGAACTTAACCTCCCTGGGATTGTTTTCAAACATTGAAGTGAATCCAGTACCAGATGAGAAGAATGAAGGAGGGATAGTTGTTGAAATAAATCTTAAAGAACAAGAGCACAAGACTGCTGAAGTAAGCACGGAATGGAACATTGTTCGCGGACATGGAGGCTATCCCACTTTGGCTTCACTGCTACCCGGTGGAACTGTTACATTTCAACATCGGAATATCAACGGATTGAATAGGTCCCTTATTGGTTCAGTAAGCAGTAGCAACCTCTACAACCTGCAGGTTCAGGATGATCTTGAGTTTAAGCTTGAGTACGTGCATCCATATCTGGATGGTGTGGACAATCCCCATGATCGCACTCTCCGGGTAGGCTGCTTTAACGGTCGAAAATTGAGTCCAGTCTTCACTGGTGGGCCAGGGGATGATGAAGTTCCCCCTGTATGGGTTGATCGCGCTGGCATGAAGGCTAATATAACAGAGAATTTGAGCCGTCGGAGCAAATTCACTTATGGTCTTGTGATGGAAGAGATAACCACACGTGATGAACAAAATCATATATGTTCAAATGGCCAAAGAGTGCTTCCCAGTGGAGGTGTCAGTGAAGATGGACCTCCAACAACTCTCAGCGGTACTGGCATTGACCATGTGGCATTTTTACAGTCAAATATCACACATGACAACACCAAGTTTGTGAATGGAACCTTAGTTGGTGAGAGGAATGTGTTTCAGGTTGACCAAGGCCTTGGCATTGGCAGCAAGTTTCCATTCTTCAACCGCCACCAGTTATCATTAACAAGATTTTTCCAGCTGAAGGAAGTGGAGGAAGGTGCTGGCAAACCACCACCACCTGTTCTTGTACTTCATGGTCACTATGGTGGCTGTGTTGGAGACCTTCCAAGTTATGATGCATTTACCCTTGGGGGTCCATATTCTGTGAGAGGTTACAACATGGGTGAGATAGGCGCCGCTAGACATATCCTCGAGCTTGCAGCTGAGCTACGGATACCGGTTAAAGGAAAAAATGTATATGCATTTGTAGAACATGGCAATGATCTTGGAAGTTCGAAGGATGTCAAGGGTAACCCAACAGAGGTATACAGGCGAGTGGGTCATGGGTCATCATATGGTGTTGGTGTCAAGTTAGGTCCAGTAAGAGTGGAATATGTTGTGGATCATAACTCTGGCACAGGTTCAACATTCTTCCGATTTGGTGACCGATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006605 GO:0006810 GO:0006886 GO:0006996 GO:0007275 GO:0008104 GO:0008150 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009653 GO:0009657 GO:0009658 GO:0009662 GO:0009707 GO:0009719 GO:0009725 GO:0009735 GO:0009790 GO:0009941 GO:0009987 GO:0010006 GO:0010033 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016043 GO:0017038 GO:0019867 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031350 GO:0031351 GO:0031354 GO:0031355 GO:0031358 GO:0031359 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0042170 GO:0042221 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0048598 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0061927 GO:0065002 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072596 GO:0072598 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Bac_surface_Ag

Protein Analysis

811

Amino Acids

88.8

Weight (kDa)

8.84

Isoelectric Point (pI)

41.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
POTRA1_3_Toc75	PF25282	146 - 236	1.4e-31	Toc75-like, POTRA domain
POTRA2_Toc75	PF25280	245 - 356	9.1e-56	Toc75-like, second POTRA domain
POTRA1_3_Toc75	PF25282	357 - 438	1.9e-24	Toc75-like, POTRA domain
Omp85	PF01103	477 - 794	1.1e-17	Omp85 superfamily domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004162)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46740 AT4G09080 AT4G09080 AT4G09080
fragaria_vesca	FvH4_3g28760 FvH4_3g28760 FvH4_3g28760 FvH4_5g29410
malus_domestica	MD03G1086500.v1.1 MD11G1095600.v1.1
prunus_persica	Prupe.6G071100_v2.0.a1
pyrus_communis	pycom03g06860 pycom11g08100 pycom11g08110
rosa_chinensis	RchiOBHm_Chr5g0052291 RchiOBHm_Chr7g0222311
rosa_laevigata	RLG00000002071 RLG00000034828
rosa_multiflora	Rmu_sc0007069.1_g000009 Rmu_sc0028377.1_g000001 Rmu_sc0032875.1_g000001 Rmu_sc0032876.1_g000001
rosa_roxburghii	Rroxscaffold_1G00028120 Rroxscaffold_3G00237120
rosa_rugosa	Rorug05G0273100 Rorug07G0207600
rosa_samantha	Rh5CG382000 Rh5DG371900 Rh7AG349900 Rh7DG346100
rosa_wichuraiana	Rw5G032630 Rw5G032670 Rw7G029530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	446
Acc36I	ACCTGC	2 cut(s)	1483, 1497
Acc65I	GGTACC	1 cut(s)	978
AccB1I	GGYRCC	4 cut(s)	242, 677, 978, 2174
AccB7I	CCANNNNNTGG	1 cut(s)	619
AccI	GTMKAC	3 cut(s)	582, 648, 2307
AccII	CGCG	7 cut(s)	115, 121, 127, 153, 536, 1362, 1665
AclWI	GGATC	2 cut(s)	805, 2391
AcoI	YGGCCR	2 cut(s)	621, 1780
AcsI	RAATTY	3 cut(s)	1024, 1693, 1712
AcuI	CTGAAG	2 cut(s)	1353, 2042
AcvI	CACGTG	2 cut(s)	714, 1751
AcyI	GRCGYC	3 cut(s)	243, 579, 2175
AfaI	GTAC	6 cut(s)	482, 980, 1263, 1526, 1843, 2070
AflIII	ACRYGT	1 cut(s)	1748
AgeI	ACCGGT	1 cut(s)	2218
AhdI	GACNNNNNGTC	2 cut(s)	559, 1131
AjnI	CCWGG	5 cut(s)	618, 828, 1171, 1227, 1627
AjuI	GAANNNNNNNTTGG	2 cut(s)	1776, 1808
AleI	CACNNNNGTG	1 cut(s)	2085
AloI	GAACNNNNNNTCC	4 cut(s)	485, 517, 1208, 1240
Alw21I	GWGCWC	5 cut(s)	527, 600, 612, 713, 1323
Alw26I	GTCTC	3 cut(s)	493, 700, 2094
AlwI	GGATC	2 cut(s)	805, 2391
Ama87I	CYCGRG	1 cut(s)	2192
AoxI	GGCC	9 cut(s)	569, 591, 621, 751, 866, 960, 1625, 1780, 1951
ApeKI	GCWGC	7 cut(s)	8, 763, 877, 1078, 1584, 1965, 2201
ApoI	RAATTY	3 cut(s)	1024, 1693, 1712
ArsI	GACNNNNNNTTYG	2 cut(s)	1591, 1623
AsiGI	ACCGGT	1 cut(s)	2218
Asp718I	GGTACC	1 cut(s)	978
AspLEI	GCGC	8 cut(s)	117, 123, 129, 245, 536, 763, 1667, 2177
AsuC2I	CCSGG	7 cut(s)	435, 573, 788, 964, 1094, 1401, 1577
AsuHPI	GGTGA	7 cut(s)	247, 682, 925, 943, 1934, 2177, 2435
AsuII	TTCGAA	2 cut(s)	654, 2276
AvaI	CYCGRG	1 cut(s)	2192
AvaII	GGWCC	6 cut(s)	448, 784, 1449, 1820, 2135, 2357
AxyI	CCTNAGG	1 cut(s)	566
BalI	TGGCCA	2 cut(s)	623, 1782
BanI	GGYRCC	4 cut(s)	242, 677, 978, 2174
BanII	GRGCYC	2 cut(s)	600, 612
BbrPI	CACGTG	2 cut(s)	714, 1751
BbsI	GAAGAC	2 cut(s)	1130, 1606
Bbv12I	GWGCWC	5 cut(s)	527, 600, 612, 713, 1323
BbvI	GCAGC	7 cut(s)	20, 775, 864, 1065, 1571, 1977, 2213
BccI	CCATC	9 cut(s)	47, 554, 751, 775, 847, 904, 1538, 1726, 1811
BceAI	ACGGC	6 cut(s)	236, 442, 671, 691, 853, 1686
BciT130I	CCWGG	5 cut(s)	620, 830, 1173, 1229, 1629
BciVI	GTATCC	1 cut(s)	2208
BcnI	CCSGG	7 cut(s)	435, 573, 788, 964, 1094, 1401, 1577
BcoDI	GTCTC	3 cut(s)	493, 700, 2094
BfaI	CTAG	2 cut(s)	939, 2181
BfmI	CTRYAG	1 cut(s)	1490
BfoI	RGCGCY	2 cut(s)	246, 2178
BfuAI	ACCTGC	2 cut(s)	1483, 1497
BfuI	GTATCC	1 cut(s)	2208
BlpI	GCTNAGC	2 cut(s)	455, 2205
Bme18I	GGWCC	6 cut(s)	448, 784, 1449, 1820, 2135, 2357
BmeRI	GACNNNNNGTC	2 cut(s)	559, 1131
BmeT110I	CYCGRG	1 cut(s)	2192
BmrI	ACTGGG	1 cut(s)	1791
BmsI	GCATC	3 cut(s)	30, 1540, 2107
BmuI	ACTGGG	1 cut(s)	1791
BpiI	GAAGAC	2 cut(s)	1130, 1606
BpmI	CTGGAG	1 cut(s)	584
Bpu1102I	GCTNAGC	2 cut(s)	455, 2205
Bpu14I	TTCGAA	2 cut(s)	654, 2276
BpuEI	CTTGAG	3 cut(s)	956, 1529, 1541
BpuMI	CCSGG	7 cut(s)	435, 573, 788, 964, 1094, 1401, 1577
BsaAI	YACGTR	3 cut(s)	714, 1528, 1751
BsaHI	GRCGYC	3 cut(s)	243, 579, 2175
BsaI	GGTCTC	2 cut(s)	493, 2094
BsaJI	CCNNGG	8 cut(s)	588, 618, 1227, 1228, 1628, 1948, 1954, 2128
BsaWI	WCCGGW	3 cut(s)	510, 735, 2218
Bse118I	RCCGGY	3 cut(s)	239, 880, 2218
Bse1I	ACTGG	8 cut(s)	814, 1259, 1610, 1624, 1797, 1849, 1993, 2360
Bse21I	CCTNAGG	1 cut(s)	566
Bse3DI	GCAATG	2 cut(s)	1114, 2266
BseBI	CCWGG	5 cut(s)	620, 830, 1173, 1229, 1629
BseDI	CCNNGG	8 cut(s)	588, 618, 1227, 1228, 1628, 1948, 1954, 2128
BseGI	GGATG	7 cut(s)	261, 858, 1507, 1531, 1549, 1639, 2287
BseMI	GCAATG	2 cut(s)	1114, 2266
BseMII	CTCAG	7 cut(s)	469, 478, 580, 1142, 1202, 1849, 2196
BseNI	ACTGG	8 cut(s)	814, 1259, 1610, 1624, 1797, 1849, 1993, 2360
BseXI	GCAGC	7 cut(s)	20, 775, 864, 1065, 1571, 1977, 2213
BsgI	GTGCAG	1 cut(s)	911
Bsh1236I	CGCG	7 cut(s)	115, 121, 127, 153, 536, 1362, 1665
Bsh1285I	CGRYCG	2 cut(s)	511, 1597
BshFI	GGCC	9 cut(s)	571, 593, 623, 753, 868, 962, 1627, 1782, 1953
BshNI	GGYRCC	4 cut(s)	242, 677, 978, 2174
BshTI	ACCGGT	1 cut(s)	2218
BsiEI	CGRYCG	2 cut(s)	511, 1597
BsiHKAI	GWGCWC	5 cut(s)	527, 600, 612, 713, 1323
BsiHKCI	CYCGRG	1 cut(s)	2192
BslFI	GGGAC	4 cut(s)	157, 431, 547, 1435
BsmAI	GTCTC	3 cut(s)	493, 700, 2094
BsmBI	CGTCTC	1 cut(s)	700
BsmFI	GGGAC	4 cut(s)	157, 431, 547, 1435
BsnI	GGCC	9 cut(s)	571, 593, 623, 753, 868, 962, 1627, 1782, 1953
Bso31I	GGTCTC	2 cut(s)	493, 2094
BsoBI	CYCGRG	1 cut(s)	2192
Bsp119I	TTCGAA	2 cut(s)	654, 2276
Bsp1286I	GDGCHC	5 cut(s)	527, 600, 612, 713, 1323
Bsp143I	GATC	7 cut(s)	67, 810, 1504, 1564, 1660, 2263, 2383
Bsp1720I	GCTNAGC	2 cut(s)	455, 2205
BspANI	GGCC	9 cut(s)	571, 593, 623, 753, 868, 962, 1627, 1782, 1953
BspCNI	CTCAG	7 cut(s)	468, 477, 579, 1141, 1203, 1848, 2197
BspFNI	CGCG	7 cut(s)	115, 121, 127, 153, 536, 1362, 1665
BspMAI	CTGCAG	1 cut(s)	1494
BspMI	ACCTGC	2 cut(s)	1483, 1497
BspPI	GGATC	2 cut(s)	805, 2391
BspT104I	TTCGAA	2 cut(s)	654, 2276
BspT107I	GGYRCC	4 cut(s)	242, 677, 978, 2174
BspTNI	GGTCTC	2 cut(s)	493, 2094
BsrDI	GCAATG	2 cut(s)	1114, 2266
BsrFI	RCCGGY	3 cut(s)	239, 880, 2218
BsrI	ACTGG	8 cut(s)	814, 1259, 1610, 1624, 1797, 1849, 1993, 2360
BssAI	RCCGGY	3 cut(s)	239, 880, 2218
BssECI	CCNNGG	8 cut(s)	588, 618, 1227, 1228, 1628, 1948, 1954, 2128
BssMI	GATC	7 cut(s)	67, 810, 1504, 1564, 1660, 2263, 2383
BssNAI	GTATAC	1 cut(s)	2308
BssNI	GRCGYC	3 cut(s)	243, 579, 2175
BssT1I	CCWWGG	4 cut(s)	588, 1948, 1954, 2128
Bst1107I	GTATAC	1 cut(s)	2308
Bst2UI	CCWGG	5 cut(s)	620, 830, 1173, 1229, 1629
Bst4CI	ACNGT	5 cut(s)	529, 694, 1411, 1595, 1872
Bst6I	CTCTTC	2 cut(s)	205, 1731
BstACI	GRCGYC	3 cut(s)	243, 579, 2175
BstBAI	YACGTR	3 cut(s)	714, 1528, 1751
BstBI	TTCGAA	2 cut(s)	654, 2276
BstDEI	CTNAG	8 cut(s)	455, 464, 566, 1128, 1211, 1835, 1915, 2205
BstEII	GGTNACC	2 cut(s)	2291, 2423
BstF5I	GGATG	7 cut(s)	261, 858, 1507, 1531, 1549, 1639, 2287
BstFNI	CGCG	7 cut(s)	115, 121, 127, 153, 536, 1362, 1665
BstH2I	RGCGCY	2 cut(s)	246, 2178
BstHHI	GCGC	8 cut(s)	117, 123, 129, 245, 536, 763, 1667, 2177
BstKTI	GATC	7 cut(s)	70, 813, 1507, 1567, 1663, 2266, 2386
BstMAI	GTCTC	3 cut(s)	493, 700, 2094
BstMBI	GATC	7 cut(s)	67, 810, 1504, 1564, 1660, 2263, 2383
BstMCI	CGRYCG	2 cut(s)	511, 1597
BstNI	CCWGG	5 cut(s)	620, 830, 1173, 1229, 1629
BstNSI	RCATGY	1 cut(s)	386
BstPI	GGTNACC	2 cut(s)	2291, 2423
BstSFI	CTRYAG	1 cut(s)	1490
BstUI	CGCG	7 cut(s)	115, 121, 127, 153, 536, 1362, 1665
BstV1I	GCAGC	7 cut(s)	20, 775, 864, 1065, 1571, 1977, 2213
BstV2I	GAAGAC	2 cut(s)	1130, 1606
BstX2I	RGATCY	1 cut(s)	810
BstXI	CCANNNNNNTGG	2 cut(s)	781, 1955
BstYI	RGATCY	1 cut(s)	810
BstZ17I	GTATAC	1 cut(s)	2308
Bsu36I	CCTNAGG	1 cut(s)	566
BsuI	GTATCC	1 cut(s)	2208
BsuRI	GGCC	9 cut(s)	571, 593, 623, 753, 868, 962, 1627, 1782, 1953
BtgZI	GCGATG	1 cut(s)	25
BtsCI	GGATG	7 cut(s)	261, 858, 1507, 1531, 1549, 1639, 2287
BtsI	GCAGTG	1 cut(s)	1391
BtsIMutI	CAGTG	5 cut(s)	472, 1391, 1617, 1804, 1816
BveI	ACCTGC	2 cut(s)	1483, 1497
CfoI	GCGC	8 cut(s)	117, 123, 129, 245, 536, 763, 1667, 2177
Cfr10I	RCCGGY	3 cut(s)	239, 880, 2218
CseI	GACGC	1 cut(s)	568
CsiI	ACCWGGT	1 cut(s)	1171
Csp6I	GTAC	6 cut(s)	481, 979, 1262, 1525, 1842, 2069
CspAI	ACCGGT	1 cut(s)	2218
CviQI	GTAC	6 cut(s)	481, 979, 1262, 1525, 1842, 2069
DdeI	CTNAG	8 cut(s)	455, 464, 566, 1128, 1211, 1835, 1915, 2205
DinI	GGCGCC	2 cut(s)	244, 2176
DpnI	GATC	7 cut(s)	69, 812, 1506, 1566, 1662, 2265, 2385
DpnII	GATC	7 cut(s)	67, 810, 1504, 1564, 1660, 2263, 2383
DrdI	GACNNNNNNGTC	1 cut(s)	446
DriI	GACNNNNNGTC	2 cut(s)	559, 1131
DseDI	GACNNNNNNGTC	1 cut(s)	446
EaeI	YGGCCR	2 cut(s)	621, 1780
Eam1104I	CTCTTC	2 cut(s)	205, 1731
Eam1105I	GACNNNNNGTC	2 cut(s)	559, 1131
EarI	CTCTTC	2 cut(s)	205, 1731
EciI	GGCGGA	4 cut(s)	312, 318, 360, 740
Ecl136II	GAGCTC	2 cut(s)	598, 610
Eco130I	CCWWGG	4 cut(s)	588, 1948, 1954, 2128
Eco147I	AGGCCT	1 cut(s)	1953
Eco24I	GRGCYC	2 cut(s)	600, 612
Eco31I	GGTCTC	2 cut(s)	493, 2094
Eco47I	GGWCC	6 cut(s)	448, 784, 1449, 1820, 2135, 2357
Eco53kI	GAGCTC	2 cut(s)	598, 610
Eco57I	CTGAAG	2 cut(s)	1353, 2042
Eco72I	CACGTG	2 cut(s)	714, 1751
Eco81I	CCTNAGG	1 cut(s)	566
Eco88I	CYCGRG	1 cut(s)	2192
Eco91I	GGTNACC	2 cut(s)	2291, 2423
EcoICRI	GAGCTC	2 cut(s)	598, 610
EcoO109I	RGGNCCY	1 cut(s)	1449
EcoO65I	GGTNACC	2 cut(s)	2291, 2423
EcoRII	CCWGG	5 cut(s)	618, 828, 1171, 1227, 1627
EcoT14I	CCWWGG	4 cut(s)	588, 1948, 1954, 2128
EcoT22I	ATGCAT	3 cut(s)	1183, 2122, 2245
EcoT38I	GRGCYC	2 cut(s)	600, 612
EgeI	GGCGCC	2 cut(s)	244, 2176
EheI	GGCGCC	2 cut(s)	244, 2176
ErhI	CCWWGG	4 cut(s)	588, 1948, 1954, 2128
Esp3I	CGTCTC	1 cut(s)	700
FalI	AAGNNNNNCTT	2 cut(s)	635, 667
FaqI	GGGAC	4 cut(s)	157, 431, 547, 1435
FauI	CCCGC	1 cut(s)	292
FauNDI	CATATG	1 cut(s)	2335
FblI	GTMKAC	3 cut(s)	582, 648, 2307
FokI	GGATG	7 cut(s)	248, 865, 1514, 1518, 1556, 1646, 2294
FriOI	GRGCYC	2 cut(s)	600, 612
FspBI	CTAG	2 cut(s)	939, 2181
GlaI	GCGC	8 cut(s)	116, 122, 128, 244, 535, 762, 1666, 2176
GsuI	CTGGAG	1 cut(s)	584
HaeII	RGCGCY	2 cut(s)	246, 2178
HaeIII	GGCC	9 cut(s)	571, 593, 623, 753, 868, 962, 1627, 1782, 1953
HgaI	GACGC	1 cut(s)	568
HhaI	GCGC	8 cut(s)	117, 123, 129, 245, 536, 763, 1667, 2177
Hin1I	GRCGYC	3 cut(s)	243, 579, 2175
Hin6I	GCGC	8 cut(s)	115, 121, 127, 243, 534, 761, 1665, 2175
HinP1I	GCGC	8 cut(s)	115, 121, 127, 243, 534, 761, 1665, 2175
HincII	GTYRAC	3 cut(s)	649, 1015, 1945
HindII	GTYRAC	3 cut(s)	649, 1015, 1945
HindIII	AAGCTT	2 cut(s)	1100, 1517
HinfI	GANTC	8 cut(s)	410, 419, 955, 967, 1126, 1132, 1255, 1606
HphI	GGTGA	7 cut(s)	247, 682, 925, 943, 1934, 2177, 2435
Hpy166II	GTNNAC	8 cut(s)	15, 583, 649, 702, 1015, 1552, 1945, 2308
Hpy188I	TCNGA	7 cut(s)	493, 553, 1131, 1212, 1428, 1707, 2417
Hpy188III	TCNNGA	7 cut(s)	475, 601, 640, 1144, 1499, 1508, 1541
Hpy8I	GTNNAC	8 cut(s)	15, 583, 649, 702, 1015, 1552, 1945, 2308
Hpy99I	CGWCG	3 cut(s)	401, 533, 1707
HpyCH4III	ACNGT	5 cut(s)	529, 694, 1411, 1595, 1872
HpyCH4IV	ACGT	4 cut(s)	713, 747, 1527, 1750
HpyF3I	CTNAG	8 cut(s)	455, 464, 566, 1128, 1211, 1835, 1915, 2205
HpySE526I	ACGT	4 cut(s)	713, 747, 1527, 1750
Hsp92I	GRCGYC	3 cut(s)	243, 579, 2175
HspAI	GCGC	8 cut(s)	115, 121, 127, 243, 534, 761, 1665, 2175
KasI	GGCGCC	2 cut(s)	242, 2174
KpnI	GGTACC	1 cut(s)	982
KroI	GCCGGC	2 cut(s)	239, 880
KroNI	GCCGGC	2 cut(s)	241, 882
Kzo9I	GATC	7 cut(s)	67, 810, 1504, 1564, 1660, 2263, 2383
LmnI	GCTCC	4 cut(s)	522, 603, 615, 1707
Lsp1109I	GCAGC	7 cut(s)	20, 775, 864, 1065, 1571, 1977, 2213
LweI	GCATC	3 cut(s)	30, 1540, 2107
MabI	ACCWGGT	1 cut(s)	1171
MaeI	CTAG	2 cut(s)	939, 2181
MaeII	ACGT	4 cut(s)	713, 747, 1527, 1750
MaeIII	GTNAC	7 cut(s)	253, 688, 1411, 2078, 2153, 2291, 2423
MalI	GATC	7 cut(s)	69, 812, 1506, 1566, 1662, 2265, 2385
MboI	GATC	7 cut(s)	67, 810, 1504, 1564, 1660, 2263, 2383
MfeI	CAATTG	1 cut(s)	625
MflI	RGATCY	1 cut(s)	810
MhlI	GDGCHC	5 cut(s)	527, 600, 612, 713, 1323
MlsI	TGGCCA	2 cut(s)	623, 1782
MluCI	AATT	8 cut(s)	74, 277, 625, 1024, 1151, 1601, 1693, 1712
MluNI	TGGCCA	2 cut(s)	623, 1782
Mly113I	GGCGCC	2 cut(s)	243, 2175
MlyI	GAGTC	5 cut(s)	413, 419, 1120, 1141, 1615
MmeI	TCCRAC	5 cut(s)	228, 576, 1685, 1850, 2077
Mox20I	TGGCCA	2 cut(s)	623, 1782
Mph1103I	ATGCAT	3 cut(s)	1183, 2122, 2245
MreI	CGCCGGCG	1 cut(s)	239
MroNI	GCCGGC	2 cut(s)	239, 880
MscI	TGGCCA	2 cut(s)	623, 1782
MseI	TTAA	8 cut(s)	744, 1185, 1220, 1308, 1515, 1590, 2003, 2223
MslI	CAYNNNNRTG	5 cut(s)	746, 779, 1250, 2085, 2259
Msp20I	TGGCCA	2 cut(s)	623, 1782
MspA1I	CMGCKG	4 cut(s)	65, 1839, 2020, 2204
MunI	CAATTG	1 cut(s)	625
MvaI	CCWGG	5 cut(s)	620, 830, 1173, 1229, 1629
MvnI	CGCG	7 cut(s)	115, 121, 127, 153, 536, 1362, 1665
NaeI	GCCGGC	2 cut(s)	241, 882
NarI	GGCGCC	2 cut(s)	243, 2175
NciI	CCSGG	7 cut(s)	435, 573, 788, 964, 1094, 1401, 1577
NdeI	CATATG	1 cut(s)	2335
NdeII	GATC	7 cut(s)	67, 810, 1504, 1564, 1660, 2263, 2383
NgoMIV	GCCGGC	2 cut(s)	239, 880
NmeAIII	GCCGAG	2 cut(s)	619, 1054
NmuCI	GTSAC	4 cut(s)	253, 688, 2078, 2423
NsiI	ATGCAT	3 cut(s)	1183, 2122, 2245
NspI	RCATGY	1 cut(s)	386
NspV	TTCGAA	2 cut(s)	654, 2276
OliI	CACNNNNGTG	1 cut(s)	2085
PaeI	GCATGC	1 cut(s)	386
PaeR7I	CTCGAG	1 cut(s)	2192
PasI	CCCWGGG	1 cut(s)	1228
PceI	AGGCCT	1 cut(s)	1953
PcsI	WCGNNNNNNNCGW	5 cut(s)	405, 537, 645, 690, 710
PdiI	GCCGGC	2 cut(s)	241, 882
PfeI	GAWTC	3 cut(s)	955, 967, 1255
PflMI	CCANNNNNTGG	1 cut(s)	619
PfoI	TCCNGGA	1 cut(s)	1092
PinAI	ACCGGT	1 cut(s)	2218
PleI	GAGTC	5 cut(s)	413, 418, 1120, 1140, 1614
PluTI	GGCGCC	2 cut(s)	246, 2178
PmaCI	CACGTG	2 cut(s)	714, 1751
PmlI	CACGTG	2 cut(s)	714, 1751
PpsI	GAGTC	5 cut(s)	413, 418, 1120, 1140, 1614
Ppu21I	YACGTR	3 cut(s)	714, 1528, 1751
PpuMI	RGGWCCY	1 cut(s)	1449
Psp124BI	GAGCTC	2 cut(s)	600, 612
Psp5II	RGGWCCY	1 cut(s)	1449
Psp6I	CCWGG	5 cut(s)	618, 828, 1171, 1227, 1627
PspCI	CACGTG	2 cut(s)	714, 1751
PspEI	GGTNACC	2 cut(s)	2291, 2423
PspGI	CCWGG	5 cut(s)	618, 828, 1171, 1227, 1627
PspPPI	RGGWCCY	1 cut(s)	1449
PspXI	VCTCGAGB	1 cut(s)	2192
PstI	CTGCAG	1 cut(s)	1494
PsuI	RGATCY	1 cut(s)	810
PvuII	CAGCTG	2 cut(s)	2020, 2204
RsaI	GTAC	6 cut(s)	482, 980, 1263, 1526, 1843, 2070
RsaNI	GTAC	6 cut(s)	481, 979, 1262, 1525, 1842, 2069
RseI	CAYNNNNRTG	5 cut(s)	746, 779, 1250, 2085, 2259
SacI	GAGCTC	2 cut(s)	600, 612
SalI	GTCGAC	1 cut(s)	647
SaqAI	TTAA	8 cut(s)	744, 1185, 1220, 1308, 1515, 1590, 2003, 2223
Sau3AI	GATC	7 cut(s)	67, 810, 1504, 1564, 1660, 2263, 2383
SbfI	CCTGCAGG	1 cut(s)	1494
SchI	GAGTC	5 cut(s)	413, 419, 1120, 1141, 1615
SdaI	CCTGCAGG	1 cut(s)	1494
SduI	GDGCHC	5 cut(s)	527, 600, 612, 713, 1323
SexAI	ACCWGGT	1 cut(s)	1171
SfaNI	GCATC	3 cut(s)	30, 1540, 2107
SfcI	CTRYAG	1 cut(s)	1490
SfoI	GGCGCC	2 cut(s)	244, 2176
Sfr274I	CTCGAG	1 cut(s)	2192
SfuI	TTCGAA	2 cut(s)	654, 2276
SgrAI	CRCCGGYG	1 cut(s)	239
SinI	GGWCC	6 cut(s)	448, 784, 1449, 1820, 2135, 2357
SlaI	CTCGAG	1 cut(s)	2192
SmiMI	CAYNNNNRTG	5 cut(s)	746, 779, 1250, 2085, 2259
SmlI	CTYRAG	4 cut(s)	971, 1508, 1520, 2192
SmoI	CTYRAG	4 cut(s)	971, 1508, 1520, 2192
SphI	GCATGC	1 cut(s)	386
Sse8387I	CCTGCAGG	1 cut(s)	1494
Sse9I	AATT	8 cut(s)	74, 277, 625, 1024, 1151, 1601, 1693, 1712
SseBI	AGGCCT	1 cut(s)	1953
SspDI	GGCGCC	2 cut(s)	242, 2174
SspMI	CTAG	2 cut(s)	939, 2181
SstI	GAGCTC	2 cut(s)	600, 612
StuI	AGGCCT	1 cut(s)	1953
StyI	CCWWGG	4 cut(s)	588, 1948, 1954, 2128
TaaI	ACNGT	5 cut(s)	529, 694, 1411, 1595, 1872
TaiI	ACGT	4 cut(s)	716, 750, 1530, 1753
TaqI	TCGA	8 cut(s)	507, 639, 648, 654, 1168, 1597, 2193, 2276
TaqII	GACCGA	1 cut(s)	812
TasI	AATT	8 cut(s)	74, 277, 625, 1024, 1151, 1601, 1693, 1712
TatI	WGTACW	2 cut(s)	480, 2068
TfiI	GAWTC	3 cut(s)	955, 967, 1255
Tru1I	TTAA	8 cut(s)	744, 1185, 1220, 1308, 1515, 1590, 2003, 2223
Tru9I	TTAA	8 cut(s)	744, 1185, 1220, 1308, 1515, 1590, 2003, 2223
TscAI	CASTG	5 cut(s)	472, 1398, 1624, 1804, 1816
TseFI	GTSAC	4 cut(s)	253, 688, 2078, 2423
TseI	GCWGC	7 cut(s)	8, 763, 877, 1078, 1584, 1965, 2201
Tsp45I	GTSAC	4 cut(s)	253, 688, 2078, 2423
TspDTI	ATGAA	6 cut(s)	280, 1293, 1653, 1688, 1770, 2063
TspGWI	ACGGA	4 cut(s)	719, 1358, 1452, 2227
TspRI	CASTG	5 cut(s)	472, 1398, 1624, 1804, 1816
Van91I	CCANNNNNTGG	1 cut(s)	619
VpaK11BI	GGWCC	6 cut(s)	448, 784, 1449, 1820, 2135, 2357
XapI	RAATTY	3 cut(s)	1024, 1693, 1712
XceI	RCATGY	1 cut(s)	386
XcmI	CCANNNNNNNNNTGG	1 cut(s)	553
XhoI	CTCGAG	1 cut(s)	2192
XmiI	GTMKAC	3 cut(s)	582, 648, 2307
XspI	CTAG	2 cut(s)	939, 2181
Zsp2I	ATGCAT	3 cut(s)	1183, 2122, 2245

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.