Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0004162

Physical Location & Seq

Genomic Coordinates Forward (+)

7959183 .. 7963011

Gene Structure 3829 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1095600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2442 bp

ATGTTCGCAGCGGCGCCCTCCTCCGCCTACCTCAACCCCAAGCCCACCATCCTCCCCTCTTCCTCCCGCACCCTAGCCCCGCCGCTCACAGCCGCCACTGCCTCTAACAGCCGCCGACCAGTCGTCAAATGCCACCTGTCTTCGAATTCTCCCTCTCCCCTTTCCCAAAACCCTAAACCCTCCTCCACTCCCCTCAAAACCCTCTCCAAAGCCCTTGCCGCCGCCTCCACCGTCACCATCATCTTCAATTGGCGATCTTTTGCTGGGAATGGTAATTCCGGCGGTGGCGGAGGTGGATTCGGCGGCGGAGGCGGAGGTGGAGGGGGAGATGGGTTCGGCGGCGGGGAAGATGGGTTTGGTAGGTTTTTGAAGAGGCTGTTGTTTGGGTCGCAGACGGCCATGGCGGACGAGCCACAGTCCCAAGAGTGGGATTCGCATGGGTTACCGGCCAACATTGTTGTCCAGCTCAACAAGCTCAGTGGGTTCAAGAAATACAAGGTCTCCGAGATCTTGTTCTTCGATCGGCGGCGGTGGAGCACCGTCGGCACCGAAGACTCGTTCTTCGAAATGGTATCCCTGAGACCTGGCGGCATTTACACCAAAGCCCAGTTGCAGAAGGAGCTCGAGAACTTAGCCAATTGCGGGATGTTCGAGAAGGTCGATTTGGAAGGGAAGACCAACCCAGATGGGACTCTGGGCGTGACGATTTCGTTTACAGAGAGCACATGGCAGTCGGCCGACAAGTTCCGATGCATCAATGTGGGTCTGATGGCGCAGTCGAAGCCAATCGAAATGGACCCGGATATGACCGAGAAGGAAAAGCTCGAGTATTTTCGGAACCAGGAGAAGGATTACAAGAGAAGGATTGATAGGGCGAGGCCGTGTTTGCTGCCGGCGCCTGTGCAGAGGGAGGTGTTGCTGATGTTGAGAGAACAAGGGAAGGTGAGCGCAAGGCTGTTGCAGAAGATTCGAGACCGGGTTCAGAAGTGGTACCAGGATGAAGGGTATGCTTGTGCACAGGTTGTGAATTTTGGGAATTTGAATACCAAGGAGGTGGTTTGTGAGGTTGTGGAAGGGGATATCACTCAGCTGCATATTCAGTTCCAGGACAAGCTGGGGAATTTCGTCGAAGGGAACACACAGATCCCCGTGGTGAAGAGGGAATTGCCTAGGCAGCTTCGCCCGGGTTATGTTTTCAACATAGAAGCAGGGAAACAAGCTCTGAGGAACATAAATTCCCTAGCTTTGTTTTCAAACATTGAAGTGAACCCACGGCCTGATGAGAAGAATGAAGGAGGTATAATTGTTGAAATAAAGCTTAAAGAACTAGAACAGAAGACAGCTGAACTTAACACGGAATGGAACATTGTTCCTGGACGCGGAGGTTATCCGACTCTGGCTTCGCTAGAGCCTGGTGGCACTGTTACATTTGAACATCGGAATCTCGGTGGCCTGAATAGATCTATTCTCGGGTCAATAACCACCAGCAACTTCTTGAATCCTCAGGATGATCTTGCTTTCAAGCTTGAGTATGTGCATCCATATCTGGATGGTGTGTACAATCCCCGCAATCGTGCTCTCCGTGTAAGCTGCTTCAACAGCCGAAAACTAAGTCCAGTCTTTACTGGTGGGCCAGGGGCCGATGAAGTACCACCTATTTGGGTTGATCGAGCTGGAGTTAAGGCTAATATAACAGAGAATTTCACCCGTCAGAGCAAATTCACCTATGGACTTGTAGTGGAAGAGATAACCACGCGTGATGAACGCAGTCATGTCTGTTCTAATGGCCAAAGAGTGTTGCCAAATGGAGGAGTAAGTGAAGATGGACCTCCAACAACTCTCAGTGGTACAGGCATTGACCGCGTGGCATTTTTACAATCAAATATCACTCGGGATAACACTAAATTTGTGAATGGAGCTATAGTTGGTCAGAGGAATATGTTTCAGGTTGACCAAGGTGTTGGCGTGGGCAGCAACTTTCCATTCTTCAACCGTCACCAGCTAACATTAACCAGATTTTTCCAGCTGAAGGAAGTCGAGGAAGGTGCTGGTAAACCACCACCGCCTGTCCTTGTACTTCACGGACACTATGGTGGCTGTATCGGAGACCTTCCAAGTTATGATACATTTACCCTTGGCGGTCCATATTCTGTGAGGGGTTACAACATGGGCGAGATAGGCGCAGCTAGAAACATCCTTGAGCTTGCTGCTGAGCTACGGATACCTGTGAAAGGTACACATGTATATGCATTTGCAGAACACGGAAATGACCTAGGAACGTCCAAGGAAGTCAAGGGTAACCCAACAGAGGTATACAGGCGATTGGGTCACGGGTCAGCTTATGGTGTTGGCGTAAAGTTAGGTTTAGTAAGAGCTGAGTATGCAGTCGACCATAACTCCGGCACAGGTGCATTATTCTTCCGTTTTGGCGAGAGGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006605 GO:0006810 GO:0006886 GO:0006996 GO:0007275 GO:0008104 GO:0008150 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009653 GO:0009657 GO:0009658 GO:0009662 GO:0009707 GO:0009719 GO:0009725 GO:0009735 GO:0009790 GO:0009941 GO:0009987 GO:0010006 GO:0010033 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016043 GO:0017038 GO:0019867 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031350 GO:0031351 GO:0031354 GO:0031355 GO:0031358 GO:0031359 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0042170 GO:0042221 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0048598 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0061927 GO:0065002 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072596 GO:0072598 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Bac_surface_Ag

Protein Analysis

814

Amino Acids

89.0

Weight (kDa)

8.78

Isoelectric Point (pI)

44.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
POTRA1_3_Toc75	PF25282	149 - 240	2e-40	Toc75-like, POTRA domain
POTRA2_Toc75	PF25280	249 - 360	9.5e-59	Toc75-like, second POTRA domain
POTRA1_3_Toc75	PF25282	361 - 442	2.7e-29	Toc75-like, POTRA domain
Omp85	PF01103	481 - 813	4.2e-20	Omp85 superfamily domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004162)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46740 AT4G09080 AT4G09080 AT4G09080
fragaria_vesca	FvH4_3g28760 FvH4_3g28760 FvH4_3g28760 FvH4_5g29410
malus_domestica	MD03G1086500.v1.1 MD11G1095600.v1.1
prunus_persica	Prupe.6G071100_v2.0.a1
pyrus_communis	pycom03g06860 pycom11g08100 pycom11g08110
rosa_chinensis	RchiOBHm_Chr5g0052291 RchiOBHm_Chr7g0222311
rosa_laevigata	RLG00000002071 RLG00000034828
rosa_multiflora	Rmu_sc0007069.1_g000009 Rmu_sc0028377.1_g000001 Rmu_sc0032875.1_g000001 Rmu_sc0032876.1_g000001
rosa_roxburghii	Rroxscaffold_1G00028120 Rroxscaffold_3G00237120
rosa_rugosa	Rorug05G0273100 Rorug07G0207600
rosa_samantha	Rh5CG382000 Rh5DG371900 Rh7AG349900 Rh7DG346100
rosa_wichuraiana	Rw5G032630 Rw5G032670 Rw7G029530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	990
AccB1I	GGYRCC	4 cut(s)	13, 545, 895, 990
AccBSI	CCGCTC	1 cut(s)	85
AccI	GTMKAC	2 cut(s)	2313, 2388
AccII	CGCG	3 cut(s)	1380, 1756, 1863
AclWI	GGATC	1 cut(s)	1138
AcoI	YGGCCR	4 cut(s)	396, 447, 735, 1786
AcsI	RAATTY	8 cut(s)	145, 1027, 1036, 1120, 1234, 1699, 1718, 1904
AcuI	CTGAAG	1 cut(s)	2048
AcyI	GRCGYC	2 cut(s)	14, 896
AfaI	GTAC	6 cut(s)	992, 1559, 1650, 1849, 2076, 2236
AfiI	CCNNNNNNNGG	9 cut(s)	426, 427, 642, 847, 1379, 1808, 2029, 2231, 2308
AflIII	ACRYGT	2 cut(s)	1754, 2239
AhdI	GACNNNNNGTC	1 cut(s)	122
AjnI	CCWGG	7 cut(s)	583, 840, 993, 1104, 1372, 1411, 1633
AjuI	GAANNNNNNNTTGG	4 cut(s)	339, 371, 647, 679
AleI	CACNNNNGTG	1 cut(s)	2091
AloI	GAACNNNNNNTCC	2 cut(s)	1220, 1252
Alw21I	GWGCWC	5 cut(s)	539, 624, 725, 1018, 1579
Alw26I	GTCTC	4 cut(s)	505, 574, 966, 2100
Alw44I	GTGCAC	1 cut(s)	1014
AlwI	GGATC	1 cut(s)	1138
Ama87I	CYCGRG	5 cut(s)	623, 824, 1183, 1469, 1890
AoxI	GGCC	9 cut(s)	396, 447, 735, 878, 1274, 1450, 1631, 1638, 1786
ApaLI	GTGCAC	1 cut(s)	1014
ApeKI	GCWGC	8 cut(s)	8, 889, 1090, 1174, 1590, 1971, 2183, 2207
ApoI	RAATTY	8 cut(s)	145, 1027, 1036, 1120, 1234, 1699, 1718, 1904
ArsI	GACNNNNNNTTYG	6 cut(s)	545, 577, 1384, 1416, 1597, 1629
Asp718I	GGTACC	1 cut(s)	990
AspA2I	CCTAGG	2 cut(s)	1169, 2272
AspLEI	GCGC	5 cut(s)	16, 775, 898, 950, 2183
AspS9I	GGNCC	5 cut(s)	796, 1631, 1638, 1826, 2141
AsuC2I	CCSGG	4 cut(s)	800, 977, 1184, 1185
AsuHPI	GGTGA	6 cut(s)	226, 955, 1165, 1696, 1714, 1988
AsuII	TTCGAA	2 cut(s)	143, 564
AvaI	CYCGRG	5 cut(s)	623, 824, 1183, 1469, 1890
AvaII	GGWCC	3 cut(s)	796, 1826, 2141
AvrII	CCTAGG	2 cut(s)	1169, 2272
AxyI	CCTNAGG	1 cut(s)	1503
BaeGI	GKGCMC	1 cut(s)	1018
BaeI	ACNNNNGTAYC	2 cut(s)	2226, 2259
BalI	TGGCCA	1 cut(s)	1788
BanI	GGYRCC	4 cut(s)	13, 545, 895, 990
BanII	GRGCYC	1 cut(s)	624
BbsI	GAAGAC	4 cut(s)	132, 558, 680, 1343
Bbv12I	GWGCWC	5 cut(s)	539, 624, 725, 1018, 1579
BbvI	GCAGC	8 cut(s)	20, 876, 1077, 1186, 1577, 1983, 2194, 2195
BccI	CCATC	8 cut(s)	56, 245, 323, 344, 680, 763, 1544, 1817
BceAI	ACGGC	3 cut(s)	411, 865, 1289
BcgI	CGANNNNNNTGC	4 cut(s)	950, 984, 2027, 2061
BciT130I	CCWGG	7 cut(s)	585, 842, 995, 1106, 1374, 1413, 1635
BciVI	GTATCC	2 cut(s)	583, 2214
BcnI	CCSGG	4 cut(s)	800, 977, 1184, 1185
BcoDI	GTCTC	4 cut(s)	505, 574, 966, 2100
BfaI	CTAG	7 cut(s)	74, 1170, 1241, 1328, 1406, 2187, 2273
BfmI	CTRYAG	1 cut(s)	1920
BfoI	RGCGCY	2 cut(s)	17, 899
BfuI	GTATCC	2 cut(s)	583, 2214
BglII	AGATCT	2 cut(s)	507, 1460
BlnI	CCTAGG	2 cut(s)	1169, 2272
BlpI	GCTNAGC	1 cut(s)	2211
Bme18I	GGWCC	3 cut(s)	796, 1826, 2141
BmeRI	GACNNNNNGTC	1 cut(s)	122
BmeT110I	CYCGRG	5 cut(s)	623, 824, 1183, 1469, 1890
BmgT120I	GGNCC	5 cut(s)	796, 1631, 1638, 1826, 2141
BmiI	GGNNCC	7 cut(s)	15, 547, 798, 839, 897, 992, 1639
BmrI	ACTGGG	1 cut(s)	601
BmsI	GCATC	3 cut(s)	740, 762, 1546
BmuI	ACTGGG	1 cut(s)	601
BpiI	GAAGAC	4 cut(s)	132, 558, 680, 1343
BpmI	CTGGAG	1 cut(s)	1695
Bpu1102I	GCTNAGC	1 cut(s)	2211
Bpu14I	TTCGAA	2 cut(s)	143, 564
BpuEI	CTTGAG	2 cut(s)	1547, 2219
BpuMI	CCSGG	4 cut(s)	800, 977, 1184, 1185
BsaHI	GRCGYC	2 cut(s)	14, 896
BsaI	GGTCTC	4 cut(s)	505, 574, 966, 2100
BsaXI	ACNNNNNCTCC	6 cut(s)	485, 515, 1908, 1938, 2381, 2411
Bsc4I	CCNNNNNNNGG	9 cut(s)	426, 427, 642, 847, 1379, 1808, 2029, 2231, 2308
Bse118I	RCCGGY	2 cut(s)	445, 892
Bse1I	ACTGG	4 cut(s)	119, 607, 1616, 1630
Bse21I	CCTNAGG	1 cut(s)	1503
BseBI	CCWGG	7 cut(s)	585, 842, 995, 1106, 1374, 1413, 1635
BseGI	GGATG	7 cut(s)	48, 651, 1003, 1513, 1537, 1555, 2193
BseLI	CCNNNNNNNGG	9 cut(s)	426, 427, 642, 847, 1379, 1808, 2029, 2231, 2308
BseMII	CTCAG	8 cut(s)	490, 569, 1100, 1214, 1517, 1855, 2202, 2367
BseNI	ACTGG	4 cut(s)	119, 607, 1616, 1630
BseRI	GAGGAG	3 cut(s)	10, 172, 1824
BseSI	GKGCMC	1 cut(s)	1018
BseX3I	CGGCCG	1 cut(s)	735
BseXI	GCAGC	8 cut(s)	20, 876, 1077, 1186, 1577, 1983, 2194, 2195
BseYI	CCCAGC	2 cut(s)	263, 1114
BsgI	GTGCAG	1 cut(s)	923
Bsh1236I	CGCG	3 cut(s)	1380, 1756, 1863
Bsh1285I	CGRYCG	2 cut(s)	523, 738
BshFI	GGCC	9 cut(s)	398, 449, 737, 880, 1276, 1452, 1633, 1640, 1788
BshNI	GGYRCC	4 cut(s)	13, 545, 895, 990
BsiEI	CGRYCG	2 cut(s)	523, 738
BsiHKAI	GWGCWC	5 cut(s)	539, 624, 725, 1018, 1579
BsiHKCI	CYCGRG	5 cut(s)	623, 824, 1183, 1469, 1890
BsiSI	CCGG	7 cut(s)	279, 446, 800, 893, 976, 1184, 2400
BslFI	GGGAC	2 cut(s)	403, 703
BslI	CCNNNNNNNGG	9 cut(s)	426, 427, 642, 847, 1379, 1808, 2029, 2231, 2308
BsmAI	GTCTC	4 cut(s)	505, 574, 966, 2100
BsmFI	GGGAC	2 cut(s)	403, 703
BsnI	GGCC	9 cut(s)	398, 449, 737, 880, 1276, 1452, 1633, 1640, 1788
Bso31I	GGTCTC	4 cut(s)	505, 574, 966, 2100
BsoBI	CYCGRG	5 cut(s)	623, 824, 1183, 1469, 1890
Bsp119I	TTCGAA	2 cut(s)	143, 564
Bsp1286I	GDGCHC	5 cut(s)	539, 624, 725, 1018, 1579
Bsp1407I	TGTACA	1 cut(s)	1557
Bsp143I	GATC	7 cut(s)	254, 507, 520, 1143, 1460, 1510, 1666
Bsp1720I	GCTNAGC	1 cut(s)	2211
Bsp19I	CCATGG	1 cut(s)	399
BspANI	GGCC	9 cut(s)	398, 449, 737, 880, 1276, 1452, 1633, 1640, 1788
BspCNI	CTCAG	8 cut(s)	489, 570, 1099, 1215, 1516, 1854, 2203, 2368
BspFNI	CGCG	3 cut(s)	1380, 1756, 1863
BspLI	GGNNCC	7 cut(s)	15, 547, 798, 839, 897, 992, 1639
BspPI	GGATC	1 cut(s)	1138
BspT104I	TTCGAA	2 cut(s)	143, 564
BspT107I	GGYRCC	4 cut(s)	13, 545, 895, 990
BspTNI	GGTCTC	4 cut(s)	505, 574, 966, 2100
BsrBI	CCGCTC	1 cut(s)	85
BsrFI	RCCGGY	2 cut(s)	445, 892
BsrGI	TGTACA	1 cut(s)	1557
BsrI	ACTGG	4 cut(s)	119, 607, 1616, 1630
BssAI	RCCGGY	2 cut(s)	445, 892
BssMI	GATC	7 cut(s)	254, 507, 520, 1143, 1460, 1510, 1666
BssNAI	GTATAC	1 cut(s)	2314
BssNI	GRCGYC	2 cut(s)	14, 896
BssT1I	CCWWGG	7 cut(s)	399, 1047, 1169, 1954, 2134, 2272, 2283
Bst1107I	GTATAC	1 cut(s)	2314
Bst2UI	CCWGG	7 cut(s)	585, 842, 995, 1106, 1374, 1413, 1635
Bst4CI	ACNGT	5 cut(s)	232, 417, 541, 1423, 1994
Bst6I	CTCTTC	4 cut(s)	64, 365, 1151, 1737
BstACI	GRCGYC	2 cut(s)	14, 896
BstAUI	TGTACA	1 cut(s)	1557
BstBI	TTCGAA	2 cut(s)	143, 564
BstC8I	GCNNGC	2 cut(s)	894, 2205
BstDSI	CCRYGG	3 cut(s)	399, 1149, 1271
BstEII	GGTNACC	2 cut(s)	441, 2297
BstENI	CCTNNNNNAGG	1 cut(s)	2229
BstF5I	GGATG	7 cut(s)	48, 651, 1003, 1513, 1537, 1555, 2193
BstFNI	CGCG	3 cut(s)	1380, 1756, 1863
BstH2I	RGCGCY	2 cut(s)	17, 899
BstHHI	GCGC	5 cut(s)	16, 775, 898, 950, 2183
BstKTI	GATC	7 cut(s)	257, 510, 523, 1146, 1463, 1513, 1669
BstMAI	GTCTC	4 cut(s)	505, 574, 966, 2100
BstMBI	GATC	7 cut(s)	254, 507, 520, 1143, 1460, 1510, 1666
BstMCI	CGRYCG	2 cut(s)	523, 738
BstNI	CCWGG	7 cut(s)	585, 842, 995, 1106, 1374, 1413, 1635
BstNSI	RCATGY	1 cut(s)	2243
BstPI	GGTNACC	2 cut(s)	441, 2297
BstSFI	CTRYAG	1 cut(s)	1920
BstSLI	GKGCMC	1 cut(s)	1018
BstUI	CGCG	3 cut(s)	1380, 1756, 1863
BstV1I	GCAGC	8 cut(s)	20, 876, 1077, 1186, 1577, 1983, 2194, 2195
BstV2I	GAAGAC	4 cut(s)	132, 558, 680, 1343
BstX2I	RGATCY	3 cut(s)	507, 1143, 1460
BstXI	CCANNNNNNTGG	3 cut(s)	1054, 1659, 1961
BstYI	RGATCY	3 cut(s)	507, 1143, 1460
BstZ17I	GTATAC	1 cut(s)	2314
BstZI	CGGCCG	1 cut(s)	735
Bsu36I	CCTNAGG	1 cut(s)	1503
BsuI	GTATCC	2 cut(s)	583, 2214
BsuRI	GGCC	9 cut(s)	398, 449, 737, 880, 1276, 1452, 1633, 1640, 1788
BtgI	CCRYGG	3 cut(s)	399, 1149, 1271
BtsCI	GGATG	7 cut(s)	48, 651, 1003, 1513, 1537, 1555, 2193
BtsI	GCAGTG	1 cut(s)	96
BtsIMutI	CAGTG	4 cut(s)	96, 484, 1419, 1849
Cac8I	GCNNGC	2 cut(s)	894, 2205
CfoI	GCGC	5 cut(s)	16, 775, 898, 950, 2183
Cfr10I	RCCGGY	2 cut(s)	445, 892
Cfr13I	GGNCC	5 cut(s)	796, 1631, 1638, 1826, 2141
Cfr9I	CCCGGG	1 cut(s)	1183
CseI	GACGC	1 cut(s)	1386
Csp6I	GTAC	6 cut(s)	991, 1558, 1649, 1848, 2075, 2235
CspCI	CAANNNNNGTGG	2 cut(s)	460, 495
CviAII	CATG	6 cut(s)	400, 437, 726, 1772, 2167, 2240
CviQI	GTAC	6 cut(s)	991, 1558, 1649, 1848, 2075, 2235
DinI	GGCGCC	2 cut(s)	15, 897
DpnI	GATC	7 cut(s)	256, 509, 522, 1145, 1462, 1512, 1668
DpnII	GATC	7 cut(s)	254, 507, 520, 1143, 1460, 1510, 1666
DriI	GACNNNNNGTC	1 cut(s)	122
EaeI	YGGCCR	4 cut(s)	396, 447, 735, 1786
EagI	CGGCCG	1 cut(s)	735
Eam1104I	CTCTTC	4 cut(s)	64, 365, 1151, 1737
Eam1105I	GACNNNNNGTC	1 cut(s)	122
EarI	CTCTTC	4 cut(s)	64, 365, 1151, 1737
EciI	GGCGGA	5 cut(s)	13, 303, 321, 327, 419
Ecl136II	GAGCTC	1 cut(s)	622
EclXI	CGGCCG	1 cut(s)	735
Eco130I	CCWWGG	7 cut(s)	399, 1047, 1169, 1954, 2134, 2272, 2283
Eco24I	GRGCYC	1 cut(s)	624
Eco31I	GGTCTC	4 cut(s)	505, 574, 966, 2100
Eco32I	GATATC	1 cut(s)	1081
Eco47I	GGWCC	3 cut(s)	796, 1826, 2141
Eco52I	CGGCCG	1 cut(s)	735
Eco53kI	GAGCTC	1 cut(s)	622
Eco57I	CTGAAG	1 cut(s)	2048
Eco81I	CCTNAGG	1 cut(s)	1503
Eco88I	CYCGRG	5 cut(s)	623, 824, 1183, 1469, 1890
Eco91I	GGTNACC	2 cut(s)	441, 2297
EcoICRI	GAGCTC	1 cut(s)	622
EcoNI	CCTNNNNNAGG	1 cut(s)	2229
EcoO65I	GGTNACC	2 cut(s)	441, 2297
EcoRI	GAATTC	1 cut(s)	145
EcoRII	CCWGG	7 cut(s)	583, 840, 993, 1104, 1372, 1411, 1633
EcoRV	GATATC	1 cut(s)	1081
EcoT14I	CCWWGG	7 cut(s)	399, 1047, 1169, 1954, 2134, 2272, 2283
EcoT22I	ATGCAT	2 cut(s)	755, 2251
EcoT38I	GRGCYC	1 cut(s)	624
EgeI	GGCGCC	2 cut(s)	15, 897
EheI	GGCGCC	2 cut(s)	15, 897
ErhI	CCWWGG	7 cut(s)	399, 1047, 1169, 1954, 2134, 2272, 2283
FaeI	CATG	6 cut(s)	403, 440, 729, 1775, 2170, 2243
FaqI	GGGAC	2 cut(s)	403, 703
FatI	CATG	6 cut(s)	399, 436, 725, 1771, 2166, 2239
FauI	CCCGC	5 cut(s)	74, 87, 335, 635, 1574
FblI	GTMKAC	2 cut(s)	2313, 2388
FokI	GGATG	7 cut(s)	35, 658, 1010, 1520, 1524, 1562, 2180
FriOI	GRGCYC	1 cut(s)	624
FspBI	CTAG	7 cut(s)	74, 1170, 1241, 1328, 1406, 2187, 2273
GlaI	GCGC	5 cut(s)	15, 774, 897, 949, 2182
GsaI	CCCAGC	2 cut(s)	267, 1118
GsuI	CTGGAG	1 cut(s)	1695
HaeII	RGCGCY	2 cut(s)	17, 899
HaeIII	GGCC	9 cut(s)	398, 449, 737, 880, 1276, 1452, 1633, 1640, 1788
HapII	CCGG	7 cut(s)	279, 446, 800, 893, 976, 1184, 2400
HgaI	GACGC	1 cut(s)	1386
HhaI	GCGC	5 cut(s)	16, 775, 898, 950, 2183
Hin1I	GRCGYC	2 cut(s)	14, 896
Hin1II	CATG	6 cut(s)	403, 440, 729, 1775, 2170, 2243
Hin6I	GCGC	5 cut(s)	14, 773, 896, 948, 2181
HinP1I	GCGC	5 cut(s)	14, 773, 896, 948, 2181
HincII	GTYRAC	2 cut(s)	1951, 2389
HindII	GTYRAC	2 cut(s)	1951, 2389
HindIII	AAGCTT	2 cut(s)	1316, 1523
HinfI	GANTC	8 cut(s)	297, 431, 554, 691, 967, 1393, 1441, 1498
HpaII	CCGG	7 cut(s)	279, 446, 800, 893, 976, 1184, 2400
HphI	GGTGA	6 cut(s)	226, 955, 1165, 1696, 1714, 1988
Hpy166II	GTNNAC	9 cut(s)	714, 1016, 1267, 1558, 1951, 2054, 2237, 2314, 2389
Hpy188III	TCNNGA	8 cut(s)	487, 625, 652, 971, 1495, 1505, 1547, 1892
Hpy8I	GTNNAC	9 cut(s)	714, 1016, 1267, 1558, 1951, 2054, 2237, 2314, 2389
Hpy99I	CGWCG	2 cut(s)	545, 1130
HpyCH4III	ACNGT	5 cut(s)	232, 417, 541, 1423, 1994
HpyCH4IV	ACGT	1 cut(s)	2279
HpySE526I	ACGT	1 cut(s)	2279
Hsp92I	GRCGYC	2 cut(s)	14, 896
Hsp92II	CATG	6 cut(s)	403, 440, 729, 1775, 2170, 2243
HspAI	GCGC	5 cut(s)	14, 773, 896, 948, 2181
KasI	GGCGCC	2 cut(s)	13, 895
KpnI	GGTACC	1 cut(s)	994
KroI	GCCGGC	1 cut(s)	892
KroNI	GCCGGC	1 cut(s)	894
Kzo9I	GATC	7 cut(s)	254, 507, 520, 1143, 1460, 1510, 1666
LmnI	GCTCC	3 cut(s)	534, 619, 1916
Lsp1109I	GCAGC	8 cut(s)	20, 876, 1077, 1186, 1577, 1983, 2194, 2195
LweI	GCATC	3 cut(s)	740, 762, 1546
MaeI	CTAG	7 cut(s)	74, 1170, 1241, 1328, 1406, 2187, 2273
MaeII	ACGT	1 cut(s)	2279
MaeIII	GTNAC	8 cut(s)	232, 441, 700, 1423, 1994, 2159, 2297, 2327
MalI	GATC	7 cut(s)	256, 509, 522, 1145, 1462, 1512, 1668
MbiI	CCGCTC	1 cut(s)	85
MboI	GATC	7 cut(s)	254, 507, 520, 1143, 1460, 1510, 1666
MfeI	CAATTG	2 cut(s)	247, 637
MflI	RGATCY	3 cut(s)	507, 1143, 1460
MhlI	GDGCHC	5 cut(s)	539, 624, 725, 1018, 1579
MlsI	TGGCCA	1 cut(s)	1788
MluI	ACGCGT	1 cut(s)	1754
MluNI	TGGCCA	1 cut(s)	1788
Mly113I	GGCGCC	2 cut(s)	14, 896
MlyI	GAGTC	3 cut(s)	548, 685, 1387
MmeI	TCCRAC	2 cut(s)	1415, 1856
Mox20I	TGGCCA	1 cut(s)	1788
Mph1103I	ATGCAT	2 cut(s)	755, 2251
MroNI	GCCGGC	1 cut(s)	892
MscI	TGGCCA	1 cut(s)	1788
MseI	TTAA	4 cut(s)	1320, 1350, 1680, 2009
MslI	CAYNNNNRTG	5 cut(s)	758, 1262, 2091, 2244, 2265
Msp20I	TGGCCA	1 cut(s)	1788
MspA1I	CMGCKG	4 cut(s)	11, 1090, 1343, 2026
MspI	CCGG	7 cut(s)	279, 446, 800, 893, 976, 1184, 2400
MunI	CAATTG	2 cut(s)	247, 637
MvaI	CCWGG	7 cut(s)	585, 842, 995, 1106, 1374, 1413, 1635
MvnI	CGCG	3 cut(s)	1380, 1756, 1863
NaeI	GCCGGC	1 cut(s)	894
NarI	GGCGCC	2 cut(s)	14, 896
NciI	CCSGG	4 cut(s)	800, 977, 1184, 1185
NcoI	CCATGG	1 cut(s)	399
NdeII	GATC	7 cut(s)	254, 507, 520, 1143, 1460, 1510, 1666
NgoMIV	GCCGGC	1 cut(s)	892
NlaIII	CATG	6 cut(s)	403, 440, 729, 1775, 2170, 2243
NlaIV	GGNNCC	7 cut(s)	15, 547, 798, 839, 897, 992, 1639
NmuCI	GTSAC	4 cut(s)	232, 700, 1994, 2327
NsiI	ATGCAT	2 cut(s)	755, 2251
NspI	RCATGY	1 cut(s)	2243
NspV	TTCGAA	2 cut(s)	143, 564
OliI	CACNNNNGTG	1 cut(s)	2091
PaeR7I	CTCGAG	2 cut(s)	623, 824
PciI	ACATGT	1 cut(s)	2239
PcsI	WCGNNNNNNNCGW	2 cut(s)	657, 1579
PdiI	GCCGGC	1 cut(s)	894
PfeI	GAWTC	5 cut(s)	297, 431, 967, 1441, 1498
PfoI	TCCNGGA	2 cut(s)	1104, 1372
Ple19I	CGATCG	1 cut(s)	523
PleI	GAGTC	3 cut(s)	548, 685, 1387
PluTI	GGCGCC	2 cut(s)	17, 899
PpsI	GAGTC	3 cut(s)	548, 685, 1387
PscI	ACATGT	1 cut(s)	2239
Psp124BI	GAGCTC	1 cut(s)	624
Psp6I	CCWGG	7 cut(s)	583, 840, 993, 1104, 1372, 1411, 1633
PspEI	GGTNACC	2 cut(s)	441, 2297
PspFI	CCCAGC	2 cut(s)	263, 1114
PspGI	CCWGG	7 cut(s)	583, 840, 993, 1104, 1372, 1411, 1633
PspN4I	GGNNCC	7 cut(s)	15, 547, 798, 839, 897, 992, 1639
PspPI	GGNCC	5 cut(s)	796, 1631, 1638, 1826, 2141
PspXI	VCTCGAGB	1 cut(s)	824
PsuI	RGATCY	3 cut(s)	507, 1143, 1460
PvuI	CGATCG	1 cut(s)	523
PvuII	CAGCTG	3 cut(s)	1090, 1343, 2026
RsaI	GTAC	6 cut(s)	992, 1559, 1650, 1849, 2076, 2236
RsaNI	GTAC	6 cut(s)	991, 1558, 1649, 1848, 2075, 2235
RseI	CAYNNNNRTG	5 cut(s)	758, 1262, 2091, 2244, 2265
SacI	GAGCTC	1 cut(s)	624
SalI	GTCGAC	1 cut(s)	2387
SaqAI	TTAA	4 cut(s)	1320, 1350, 1680, 2009
Sau3AI	GATC	7 cut(s)	254, 507, 520, 1143, 1460, 1510, 1666
Sau96I	GGNCC	5 cut(s)	796, 1631, 1638, 1826, 2141
SchI	GAGTC	3 cut(s)	548, 685, 1387
SduI	GDGCHC	5 cut(s)	539, 624, 725, 1018, 1579
SfaNI	GCATC	3 cut(s)	740, 762, 1546
SfcI	CTRYAG	1 cut(s)	1920
SfoI	GGCGCC	2 cut(s)	15, 897
Sfr274I	CTCGAG	2 cut(s)	623, 824
SfuI	TTCGAA	2 cut(s)	143, 564
SinI	GGWCC	3 cut(s)	796, 1826, 2141
SlaI	CTCGAG	2 cut(s)	623, 824
SmaI	CCCGGG	1 cut(s)	1185
SmiMI	CAYNNNNRTG	5 cut(s)	758, 1262, 2091, 2244, 2265
SmlI	CTYRAG	4 cut(s)	623, 824, 1526, 2198
SmoI	CTYRAG	4 cut(s)	623, 824, 1526, 2198
SspDI	GGCGCC	2 cut(s)	13, 895
SspMI	CTAG	7 cut(s)	74, 1170, 1241, 1328, 1406, 2187, 2273
SstI	GAGCTC	1 cut(s)	624
StyI	CCWWGG	7 cut(s)	399, 1047, 1169, 1954, 2134, 2272, 2283
TaaI	ACNGT	5 cut(s)	232, 417, 541, 1423, 1994
TaiI	ACGT	1 cut(s)	2282
TaqII	GACCGA	1 cut(s)	824
TatI	WGTACW	2 cut(s)	1557, 2074
TfiI	GAWTC	5 cut(s)	297, 431, 967, 1441, 1498
Tru1I	TTAA	4 cut(s)	1320, 1350, 1680, 2009
Tru9I	TTAA	4 cut(s)	1320, 1350, 1680, 2009
TscAI	CASTG	4 cut(s)	103, 484, 1426, 1849
TseFI	GTSAC	4 cut(s)	232, 700, 1994, 2327
TseI	GCWGC	8 cut(s)	8, 889, 1090, 1174, 1590, 1971, 2183, 2207
Tsp45I	GTSAC	4 cut(s)	232, 700, 1994, 2327
TspDTI	ATGAA	4 cut(s)	1014, 1305, 1659, 1776
TspGWI	ACGGA	6 cut(s)	1370, 1571, 2097, 2233, 2277, 2411
TspMI	CCCGGG	1 cut(s)	1183
TspRI	CASTG	4 cut(s)	103, 484, 1426, 1849
VneI	GTGCAC	1 cut(s)	1014
VpaK11BI	GGWCC	3 cut(s)	796, 1826, 2141
XagI	CCTNNNNNAGG	1 cut(s)	2229
XapI	RAATTY	8 cut(s)	145, 1027, 1036, 1120, 1234, 1699, 1718, 1904
XceI	RCATGY	1 cut(s)	2243
XhoI	CTCGAG	2 cut(s)	623, 824
XmaI	CCCGGG	1 cut(s)	1183
XmaJI	CCTAGG	2 cut(s)	1169, 2272
XmiI	GTMKAC	2 cut(s)	2313, 2388
XspI	CTAG	7 cut(s)	74, 1170, 1241, 1328, 1406, 2187, 2273
Zsp2I	ATGCAT	2 cut(s)	755, 2251

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.